1. Nephelometric determination of carbohydrate deficient transferrin
- Author
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J. Y. Benard, Jacques Weill, M Martin, François Schellenberg, and E Cacès
- Subjects
Adult ,Male ,Adolescent ,Clinical Biochemistry ,Carbohydrate deficient transferrin ,Alcohol ,Alcohol treatment ,Sensitivity and Specificity ,chemistry.chemical_compound ,Nephelometry and Turbidimetry ,Humans ,chemistry.chemical_classification ,Chromatography ,Isoelectric focusing ,Biochemistry (medical) ,Transferrin ,Middle Aged ,Chromatography, Ion Exchange ,Alcoholism ,Investigation methods ,Isoelectric point ,chemistry ,Regression Analysis ,Isoelectric Focusing ,Nephelometry - Abstract
We describe a technique for measuring carbohydrate-deficient transferrin (CDT) in serum. Serum transferrin fractions are separated by anion-exchange chromatography on microcolumns. Sialic acid-deficient transferrin fractions are collected in the eluate, and transferrin is then quantified by a rate-nephelometric technique. Imprecision (CV) was 4-5% within-run and 7-9% between runs (n = 15). Comparison with an isoelectric focusing-immunofixation method for transferrin index (x) yielded y = 761x + 7, Sy/x = 39 mg/L. Assay of sera from 90 abstainers or moderate consumers of alcohol showed that 81 (90%) had CDT concentrations between 30 and 70 mg/L. Among 74 alcoholics admitted to an alcohol treatment center, 54 (73%) had CDT > 70 mg/L, i.e., the diagnostic sensitivity was 73% at a specificity of 90% (area under receiver-operator characteristic curve = 0.891).
- Published
- 1996