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Constituents of cigarette smoke are known to be carcinogens. Additionally, there is mounting evidence that the liver is an organ susceptible to tobacco carcinogenicity. Nicotine, the primary constituent of tobacco, plays a role in cancer progression. In our previous study, it was found that nicotine enhances the proliferation of a human normal fetal hepatic (WRL68) cell due to the activation of p53 mutation at Ser249 (p53-RS)/STAT1/CCND1 signaling pathway. Here, we further elucidated the mechanism of regulating this pathway. Firstly, dose-dependent increase of SETDB1 protein level in WRL68 cells upon exposure to nicotine (1.25, 2.5, and 5 μM), significantly enhanced cellular proliferation. In addition, the upregulation of SETDB1 protein was necessary for the nuclear translocation of p53-RS to establish a ternary complex with STAT1 and SETDB1, which facilitated p53-RS di-methylation at K370 (p53-RS/K370me2). After that, the activation of CCND1/PI3K/AKT pathway was initiated when STAT1 stability was enhanced by p53-RS/K370me2, ultimately resulting in cell proliferation. Altogether, the study revealed that the increase in SETDB1 expression could potentially have a significant impact on the activation of CCND1/PI3K/AKT pathway through p53-RS/K370me2, leading to the proliferation of WRL68 cells induced by nicotine, which could contribute to hepatocellular carcinoma for smokers. Besides, the results of this study provided a foundation for the development of anticancer therapies for cancers associated with tobacco use.

The rock mass of coal seam floor plays a crucial role in blocking the uplift and runoff of the underlying confined water in the course of mining under water pressure. Accurately assessing its water resistance performance, which is influenced by numerous factors, is important for preventing accidents caused by inrushing water. This study focuses on a coal mine in the Ningwu coalfield in North China. We establish an evaluation system for the water resistance performance of coal seam floor rock mass, using indices including rock mass thickness, lithological combination characteristics, rock mass quality, structure complexity and bedding plane count. A variable weight–normal cloud (VW–NC) model is formulated to quantitatively evaluate the water resistance performance of the coal seam floor rock mass. Comparative analysis with actual mining conditions and other models reveals that the VW-NC model provides the most precise and accurate results. The study finds that the primary influencing factors on the water resistance performance are weak structural planes, including faults, joints, and bedding planes. The proportions of strong, relatively strong, relatively weak, and weak zones in research area are 20.41%, 59.88%, 12.38%, and 7.33%, respectively.

Developing an OER electrocatalyst that balances high performance with low cost is crucial for widely adopting PEM water electrolyzers. Ru-based catalysts are gaining attention for their cost-effectiveness and high activity, positioning them as promising alternatives to Ir-based catalysts. However, Ru-based catalysts can be prone to oxidation at high potentials, compromising their durability. In this study, we utilize a simple synthesis method to synthesize a SnO2, Nb2O5, and RuO2 composite catalyst (SnO2/Nb2O5@RuO2) with multiple interfaces and abundant oxygen vacancies. The large surface area and numerous active sites of the SnO2/Nb2O5@RuO2 catalyst lead to outstanding acidic oxygen evolution reaction (OER) performance, achieving current densities of 10, 50, and 200 mA cm−2 at ultralow overpotentials of 287, 359, and 534 mV, respectively, significantly surpassing commercial IrO2. Moreover, incorporating Nb2O5 into the SnO2/Nb2O5@RuO2 alters the electronic structure at the interfaces and generates a high density of oxygen vacancies, markedly enhancing durability. Consequently, the membrane electrode composed of SnO2/Nb2O5@RuO2 and commercial Pt/C demonstrated stable operation in the PEM cell for 25 days at an industrial current density of 1 A cm−2. This research presents a convenient approach for developing a highly efficient and durable Ru-based electrocatalyst, underscoring its potential for proton exchange membrane water electrolysis.

The 20 μg (0–1–6 month) hepatitis B virus (HBV) vaccination is widely recommended for HBV vaccine-naïve immune adults in China. However, suboptimal protective responses may occur due to dose-series incompletion. We aim to investigate the immunogenicity of a 60 μg HB vaccine with a 0–2 month series among HBV vaccine-naïve immune adults aged 25–55 to assess potential alternative approaches for HB immunization. A two-center randomized controlled trial was carried out. Participants were randomly allocated to either the 20 μg (0–1–6 month) or the 60 μg (0–2 month) regimen. Blood samples were collected eight weeks after the final injection to measure the antibodies. A total of 583 adults (289 in the 20 μg regimen and 294 in the 60 μg regimen) were included. The seroprotection rates (SPRs) were 97.23% and 93.54% in the 20 μg and 60 μg regimens, respectively (p = 0.0261), and the geometric mean concentrations were 600.76 mIU/mL and 265.68 mIU/mL, respectively (p < 0.0001). The immunogenicity of the 60 μg regimen decreased significantly with age, particularly in adults aged 40 and older. The 60 μg regimen may be beneficial for adults under 40, especially those with poor compliance or in urgent need of immunization.

Severe acne is a chronic inflammatory skin condition that is affected by both genetic and environmental factors. DNA methylation is associated with a variety of inflammatory skin diseases, but its role in severe acne is unclear. In this study, we conducted a two-stage epigenome correlation study using 88 blood samples to identify disease-related differential methylation sites. We found close associations between the DNA methylation at 23 differentially methylated sites (DMSs) and severe acne, including PDGFD, ARHGEF10, etc. Further analysis revealed that differentially methylated genes (PARP8 and MAPKAPK2) were also expressed differently between severe acne and health control groups. These findings lead us to speculation that epigenetic mechanisms may play an important role in the pathogenesis of severe acne.

The deformation behavior of a high-strength Mg-Al-Sn-Zn alloy under loading reversals has been thoroughly examined through a combination of experimental measurements and crystal plasticity modeling. We focused on an age-treated alloy fortified by distributed Mg2Sn particles and Mg17Al12 precipitates, which underwent two distinct loading cycles: tension-compression-tension (TCT) and compression-tension-compression (CTC), aligned with the extrusion direction (ED). The initial and deformed microstructures of the alloy were analyzed using the electron backscattering diffraction (EBSD) technique. Notably, the alloy displays tensile and compressive yield strengths (YS) of 215 MPa and 160 MPa, respectively, with pronounced anelastic behavior observed during unloading and reverse loading phases. Utilizing the elasto-viscoplastic self-consistent model incorporating a twinning–detwinning scheme (EVPSC-TDT), the cyclic stress–strain responses and resultant textures of the alloy were accurately captured. The predicted alternation between various slip and twinning modes during plastic deformation was used to interpret the observed behaviors. It was found that prismatic slip plays an important role during the plastic deformation of the studied alloy, and its relative activity in tensile loading processes accounts for up to ~66% and ~67% in the TCT and CTC cases, respectively. Moreover, it was discerned that detwinning and twinning behaviors are predominantly governed by stresses within the parent grain, and they can concurrently manifest during the reverse tensile loading phase in the TCT case. After cyclic deformation, the area fractions of residual twins were determined to be 7.51% and 0.93% in the TCT and CTC cases, respectively, which is a result of the varied twinning–detwinning behavior of the alloy in different loading paths.

In this study, we investigated the micromechanical deformation and damage behavior of commercially extruded and additively manufactured 316L stainless steels (AMed SS316L) by combining experimental examinations and crystal plasticity modeling. The AMed alloy was fabricated using the laser powder bed fusion (LPBF) technique with an orthogonal scanning strategy to control the directionality of the as-fabricated material. Optical microscopy and electron backscatter diffraction measurements revealed distinct grain morphologies and crystallographic textures in the two alloys. Uniaxial tensile test results suggested that the LPBFed alloy exhibited an increased yield strength, reduced elongation, and comparable ultimate tensile strength in comparison to those of the extruded alloy. A microstructure-based crystal plasticity model was developed to simulate the micromechanical deformation behavior of the alloys using representative volume elements based on realistic microstructures. A ductile fracture criterion based on the microscopically dissipated plastic energy on a slip system was adopted to predict the microscopic damage accumulation of the alloys during plastic deformation. The developed model could accurately predict the stress–strain behavior and evolution of the crystallographic textures in both the alloys. We reveal that the increased yield strength in the LPBFed alloy, compared to that in the extruded alloy, is attributed to the higher as-manufactured dislocation density and the cellular subgrain structure, resulting in a reduced elongation. The presence of annealing twins and favorable texture in the extruded alloy contributed to its excellent elongation, along with a higher hardening rate owing to twin–dislocation interactions during plastic deformation. Moreover, the grain morphology and defect state (e.g., dislocations and twins) in the initial state can significantly affect strain localization and damage accumulation in alloys.

Different ageing treatments have been developed to achieve targeted properties in aluminum alloys through altering microstructures. However, there is a lack of understanding regarding the effect of ageing treatments on the formability of these alloys. In this study, we employed crystal plasticity finite element (CPFE) modeling, in conjunction with the Marciniak-Kuczynski (M-K) approach, to investigate the effects of ageing treatments on the mechanical properties and formability of AA6061 aluminum alloy. The as-received sheet was in the T6 heat treatment state, which was subjected to artificial ageing and pre-ageing, respectively, to achieve two age-hardened alloys with modified precipitation states. The microstructures and crystallographic textures of the three alloys were measured using the electron backscattering diffraction (EBSD) technique, and uniaxial tensile tests were performed along the rolling direction (RD), transverse direction (TD), and diagonal direction (DD, 45° to the RD) for each alloy. The forming limit curve (FLC) of the as-received alloy was determined using the Nakazima test. The dependence of mechanical strength, tensile ductility, and work-hardening behavior on the ageing treatments was clarified. Then, the tensile test results were utilized to calibrate the modeling parameters used in the CPFE model, whereas the FLC predictability of the developed model was validated with the experimental one. In the formability analysis, the effects of the ageing treatment on the FLC exhibit a notable dependency on loading paths, and the pre-aged alloy exhibits better formability than the other two at the plane strain tension state, thanks to its high work-hardening levels. In addition, the deformed textures along the different loading paths and the effects of the initial texture on the FLC are also discussed.

The ship-generated wave causes erosive damage to the slopes of inland waterways and confined waters such as the coastal zone. This critical issue is essentially associated with the navigational safety and sustainable development of coastlines, so it is vital to predicting the maximum wave height of the ship-generated wave (Hm) in the coastline of confined waters. The prediction equations of prior research works are mostly based on measured data and multiple regression analysis; however, this paper aims to propose a novel methodology to predict the maximum wave height of ship-generated waves in confined waters. The maximum wave height caused by a self-propelled ship under various conditions with confined water is measured by employing a water flume. Furthermore, the relationships of functions in the prediction model equation are appropriately derived by dimensional analysis, and the prediction equation model is then solved via the particle swarm optimization algorithm (PSOA). The experimental results reveal that the maximum wave height of the ship-generated wave is seriously affected by the water depth of the channel (hc), the navigation speed of the ship (Vc), and the distance from the forecast point to the navigation line of the ship (Sc). In addition, the maximum wave height grows and then lessens, which touches its peak point in the region close to Frh = 1. The dimensionless analysis indicates that the large wave height of the ship-generated wave can be expressed as a function of the channel depth (hc), ship speed (Vc), and the distance from the measurement point to the ship’s navigation line (Sc) as; HmSc=1Frh2f(gScVc 2). Subsequently, the specific prediction equation is determined by a regression model according to the measured data under various working conditions. By adopting the model expression equation, namely, HmFrh2Sc=k1(gScVc 2)k2, the coefficients k1 and k2 are evaluated via the PSOA, and the relationship between the water depth of the channel and the coefficients is suitably outlined to obtain the maximum wave height prediction model equations for ship-generated waves.

Abstract Background The alcohol consumption pattern, alcoholic liver disease (ALD) prevalence and related risk factors among alcohol drinkers in Beijing haven’t been fully elucidated. Hence, a cross-sectional study was conducted to investigate potential link among these factors. Methods A two-stage stratified cluster sampling was carried out in Beijing. All participants were 25 years of age or older, possessed with medical insurance, and lived in Beijing for over 6 months. As part for this investigation, participants were asked to answer a questionnaire and undergo physical examination. The questionnaire included demographic information, alcohol intake, and medical history. The physical examination included physical and Fibrotouch tests. Moreover, 10 ml blood sample was collected from each subject to examine liver functions, perform routine blood, Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV). Results Overall, 74,988 residents participated in our study. The proportion of current drinkers among all participants was 46.10%. The differences in gender, region, age group, education, annual household income, and occupation among lifetime abstainers, former drinkers, non-weekly and weekly drinkers were significantly different (P

Abstract Background The incidence of hepatitis A virus (HAV) infection is low in Beijing, China, but the risk of outbreaks still exists. It is difficult to identify possible sources of infection among sporadic cases based on a routine surveillance system. Therefore, a more effective surveillance system needs to be established. Methods The epidemiological data of hepatitis A were obtained from a routine surveillance system. Patients with HAV confirmed at the local hospitals were asked to complete a questionnaire that included additional case information and possible sources of infection. Serum and fecal specimens were also collected for testing HAV RNA by polymerase chain reaction. In addition, the 321-nucleotide segment of the VP1/2A junction region was sequenced to determine the HAV genotype. Results In 2019, 110 HAV cases were reported in Beijing, with an incidence rate of 0.51/100,000. 61(55.5%) of these patients were male. The greatest proportion of these patients were aged from 30 to 60 years. The rate was lower in suburban and rural areas compared to urban areas. Contaminated food consumption, particularly seafood consumption, was the primary potential source of infection. Among the 16 specimens of confirmed HAV cases that could be sequenced, 93.8% were HAV IA, and 6.3% were HAV IB. In addition, the samples collected from all HAV sequences in this investigation showed 89.4–100% nucleotide homology. Two groups (each with three sporadic cases) showed 100% nucleotide homology. The three sporadic cases in one group had the same possible source of infection: contaminated salad with raw vegetables and seafood. In the other group, the three sporadic cases did not have an epidemiological connection. Conclusions In a low HAV prevalent area, such as in Beijing, incorporating molecular epidemiology into the routine surveillance system could help inform possible clusters of outbreaks and provide support for earlier control of HAV transmission. Nevertheless, increased sampling from detected cases and improved specimen quality are needed to implement such a system.

Extensive studies have been conducted in regards to estimating the useful life of power electronic converters in order to prevent failure related downtime. The state-of-the-art of converter reliability is based on the physics-of-failure approach, which involves some concerns such as the uncertainty introduced by extrapolating the accelerated test results to other usage conditions, which results in failure probability distributions inhibiting a variance of several years. In order to overcome this issue, the state-of-the-art is currently seeing an increase in research output, which are in favour of basing the reliability studies on physics-of-degradation based methods. The existing methods are restricted to only consider a single component and due to the impracticability of monitoring each single component, there is an essential need for methods, which can monitor the health state of the entire converter. This paper proposes a method which uses the converter operating efficiency to monitor parameter shifts of multiple components, which can be used to gain precision when stating the converter lifetime. A supervised classifier is used to detect the end-of-life while simultaneously coping with the fundamental issue of the loading influencing the efficiency. The method proves to have high accuracy even when measuring inaccuracies are taken into account.

Objective: This study investigated effects and underlying mechanisms of Qingda granules (QDG) on cardiac inflammation in spontaneously hypertensive rats (SHRs). Methods: Twelve SHRs (17 weeks old) were randomly divided into SHR and SHR + QDG groups, with six rats in each group. We also used six 17-week-old Wistar Kyoto (WKY) rats as the WKY group. While rats in the SHR + QDG group were administered QDG (0.8 g/kg/d) for eight weeks, those in the WKY and SHR groups were administered an equal volume of normal saline. Blood pressure was then monitored weekly. Subsequently, hematoxylin and eosin (HE) staining was used to detect pathological changes in the cardiac tissue. Besides, enzyme-linked immunosorbent assay (ELISA) was used to detect the serum content of inflammatory cytokines. Subsequently, real-time quantitative polymerase chain reaction and immunohistochemical (IHC) staining were conducted to determine the expression of inflammatory cytokines and inflammatory cell infiltration, including the activation of the MCP-1/CCR2 signaling pathway. Results: As observed, QDG inhibited the elevation of systolic blood pressure, diastolic blood pressure, and mean arterial pressure in the understudied SHRs. HE staining showed that this drug attenuated pathological changes and inflammatory cell infiltration of cardiac tissue samples in the SHRs. However, ELISA and IHC confirmed a reduction in the expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the serum and cardiac tissue of SHRs with QDG treatment. Additionally, QDG treatment significantly attenuated protein levels of interferon-γ, CD3, and Mac-2 in the cardiac tissue samples of SHRs. Moreover, mRNA and protein expressions of monocyte chemoattractant protein 1 (MCP-1) and chemotactic cytokine receptor 2 (CCR2), which were upregulated in cardiac tissue samples of SHRs, became downregulated through treatment with QDG. Conclusion: Therefore, by decreasing the levels of inflammatory cytokines and inflammatory cell infiltration through suppression of the MCP-1/CCR2 signaling pathway, QDG treatment attenuated blood pressure and cardiac inflammatory changes in SHRs.

Crude sophorolipids (SLs) have been proven to perform varying degrees of inhibitory effects on different pathogenic bacteria. However, systematic comparative studies of pure lactonic sophorolipid (LSL) among different types of bacteria are few. In this study, the antibacterial effects and mechanisms of LSL on pathogenic bacteria of Staphylococcus aureus, Lactobacillus sp., Pseudomonas aeruginosa, and Escherichia coli were investigated. Bacteriostatic circle, antibacterial rate, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of LSL on different pathogenic bacteria were measured. Then, the antibacterial mechanisms of LSL on S. aureus and P. aeruginosa were explored using ultrastructural observation, cell membrane permeability analysis, intracellular ATP content determination, and extracellular UV absorption detection. With the minimum MIC and MBC values of 0.05 and 0.20 mg/ml, LSL exhibited the best inhibitory effect against S. aureus, followed by P. aeruginosa. LSL showed no significant inhibitory effect on E. coli and Lactobacillus sp. For both S. aureus and P. aeruginosa, LSL achieved bacteriostatic and bactericidal effects by destroying the cell wall, increasing the permeability of the cell membrane and leading to the flow out of intracellular contents. However, the action mode and action intensity of LSL on the cell wall and membrane of these two bacteria were significantly different. LSL had a greater influence on the cell membrane of S. aureus by “leaking,” while it exhibited a stronger effect on the cell wall of P. aeruginosa by “blasting.” These results contributed to a better understanding of the relationship between LSL and different bacterial cell structures, further suggesting the conclusion that LSL might be used for the targeted treatment of special pathogenic bacteria.

Objectives: To assess the prevalence of hepatitis B virus (HBV) infection among married individuals and to analyze the associated risk factors of HBV infection in vaccinated and unvaccinated groups in 2006 and 2014. Methods: Information of married individuals aged over 16 y with a clear HBV vaccination status was extracted from the database constructed by the Beijing Center for Disease Prevention and Control from population-based investigation. A structured questionnaire was employed to collect demographic information, vaccinated history, and other related influence information of HBV of participants. Pearson chi-square test, Fisher's test, and logistic regression were used to explore the risk factors of HBV infection. Results: A total of 2874 individuals in 2006 and 1622 individuals in 2014 were enrolled in our study. The mean age of individuals was 49.30 and 46.68 y in 2006 and 2014, respectively. The overall positive rates of HBsAg, anti-HBc, and single anti-HBs were 4.80%, 43.01%, and 5.78% in 2006, which were 4.69%, 38.22%, and 14.18% in 2014, respectively. For vaccinated individuals, age was significantly correlated with anti-HBc in 2014 (40 < age ≤50 versus age ≤30, relative risk (RR) = 3.03, 95% confidence interval (95%CI) = 1.04–8.84). Gender [male versus female, RR = 0.60, 95%CI = 0.36–1.00 (2006); RR = 0.71, 95%CI = 0.52–0.97 (2014)] and age (in 2006) were found to be significantly associated with single anti-HBs. For unvaccinated individuals, HBsAg positivity was statistically significant correlated with gender [RR = 1.47, 95%CI = 1.04–2.06, in 2006], residence (urban versus rural, RR = 0.40, 95%CI = 0.24–0.67, in 2006; RR = 0.58, 95%CI = 0.34–0.99, in 2014), sharing syringes [RR = 3.75, 95%CI = 1.33–10.63 (in 2006); RR = 2.07, 95%CI = 1.26–3.41 (in 2014)], infected wives (RR = 1.97, 95%CI = 1.28–3.05, in 2006), and infected husbands (RR = 2.19, 95%CI = 1.25–3.82, in 2006). Anti-HBc positivity was significantly associated with gender [RR = 1.19, 95%CI = 1.10–1.30 (in 2006); RR = 1.24, 95%CI = 1.09–1.40 (in 2014)], age (in 2006 and 2014), endoscopic medicine treatment [RR = 1.16, 95%CI = 1.03–1.32 (in 2006), RR = 1.21, 95%CI = 1.01–1.45 (in 2014)], sharing syringes (RR = 1.43, 95%CI = 1.25–1.64, in 2014), body piercing (RR = 0.84, 95%CI = 0.75–0.93, in 2006), infected wives (RR = 1.32, 95%CI = 1.18–1.47, in 2006), and infected husbands (RR = 1.39, 95%CI = 1.22–1.59, in 2006). Anti-HBs positivity was associated with age (in 2006 and 2014). Conclusions: Prevalence of HBV infection was lower in 2014 than in 2006 according to HBsAg and anti-HBc positivity. Unvaccinated individuals faced much more risk of HBV infection than those of vaccinated.

With the increasing penetration of renewable power, its reliability and cost-effective production are becoming more important. A filter is inserted between the grid-connected inverter and the power grid to reduce the PWM switching harmonics, which may become a fragile part seen from the power electronics converter perspective. As the grid-connected inverter is typically designed with additional reactive power capability, this paper tries to investigate the additional stresses of the filter capacitor introduced by the reactive power injection. According to an electro-thermal stress evaluation, the time-to-failure distribution of a single LCL filter capacitor is investigated in detail. Moreover, the increasing equivalent series resistance along with the operational period is taken into account. Aiming towards a system-level reliability analysis, a Weibull distribution based reliability of an individual capacitor can be linked to the reliability of a capacitor bank by using a reliability block diagram. A case study of a 2 MW wind power converter shows that the lifetime is significantly reduced from the individual capacitor to the capacitor bank. Besides, over-excited reactive power injection further reduces the lifetime of the LCL filter capacitors.

Power devices are among the reliability-critical components in the Photovoltaic (PV) inverter, whose failures are normally related to the thermal stress. Therefore, thermal modeling is required for estimating the thermal stress of the power devices under long-term operating conditions of the PV inverter, i.e., mission profile. Unfortunately, most of the thermal models developed for the power device are not suitable for a long-term thermal stress analysis (e.g., days to months), and there is usually a trade-off between the model accuracy and the computational efficiency. To address this challenge, a reduced-order thermal model for PV inverters is proposed in this paper, where the model simplification is based on the thermal impedance characteristic and the mission profile dynamics. The modeling accuracy is evaluated by comparing the estimated thermal stress with the experimental results from a PV inverter test-bench, where daily mission profiles with various dynamics are tested. According to the results, the proposed method offers a relatively high model accuracy (similar to the full-order thermal model) while the computational efficiency is improved significantly, making it suitable for long-term thermal stress modeling applications.

Abstract Background Hepatitis B virus (HBV) infection is a major public health problem in China. Over a decade has passed since the last National Hepatitis Seroepidemiological Survey was conducted in 2006. The lack of updated data on hepatitis B in China makes assessing the current prevalence and burden of the disease inadequate. In response to the above situation, a systematic review and meta-analysis was conducted to provide a better understanding of hepatitis B epidemiology in the general population of China. Methods A systematic search was conducted in international databases (Medline through PubMed, EMBASE, Cochrane, Web of Science) and national databases (CBM, CNKI, WanFang Data) to retrieve primary studies published between January 1, 2013 and December 31, 2017. The pooled prevalence of HBV infection and 95% confidence intervals were calculated. Quality assessment, heterogeneity testing and publication bias assessment were also performed. Results Of the 27 studies included in the meta-analysis, the pooled estimated prevalence of HBV infection in the general population of China from 2013 to 2017 was 6.89% (95% CI:5.84–7.95%), which could be extrapolated to an estimated population of 84 million living with HBsAg in 2018. The prevalence of HBV infection in males was higher than that in females (5.88% vs 5.05%), and rural areas had a higher prevalence than urban areas (5.86% vs 3.29%). The highest prevalence of HBV infection was reported in Western provinces (8.92, 95% CI: 7.19–10.64%). In adults older than 20 years, the prevalence of HBV infection was approximately 7%, which was higher than that in children. Conclusion The prevalence of HBV infection in the general population of China was classified as higher intermediate prevalence (5–7.99%), of which more than 90% of the HBV infection population included adults older than 20 years. The blocking of mother-to-infant hepatitis B transmission and plans involving timely birth dose of hepatitis B vaccine within 24 h should be implemented. Additionally, improving the quality of life and survival rate of the infected population through antiviral therapy and high-risk adult vaccination will be the priority of our future work. Moreover, various control measures should be implemented in different provinces across China.

Backgroud: Beijing was hyper-endemic for hepatitis A until the 1990s and has been vaccinating against hepatitis A since 1994. The objective is to study the epidemiology and changes of antibody level of hepatitis A from 1990 to 2017. Methods: A multistage randomized cluster sampling serological cross-sectional study was conducted in individuals over one year old in 1992, 2006 and 2014 in Beijing. Venous blood samples were collected to test anti-HAV antibody. The incidence data of hepatitis A were obtained from National Notifiable Disease Reporting System (NNDRS) and CDC statistics in Beijing. The vaccination data of hepatitis A immunization were acquired from Beijing Immune Information System. Results: From 1990 to 2017, the reported incidence rate of HAV in Beijing declined from 59.41/100,000 in 1990 to 0.80/100,000 in 2017. The average age of HAV infection was postponed from individuals under 20 years old to individuals over 20 years old. After hepatitis A vaccine was introduced to Beijing, the outbreak of hepatitis A decreased sharply. Adjusted anti-HAV positive rate in general population was 68.23%, 81.73% and 82.47% respectively in 1992, 2006 and 2014. Due to hepatitis A vaccination conducted in children, the anti-HAV positive rate in individuals under 20 years old increased from 1992 to 2014, while in individuals over 20, this rate was barely changed. The coverage rate in target population was higher than 99% after hepatitis A vaccine was integrated into Expanded Program on Immunization (EPI). Conclusion: Incidence rate of hepatitis A in Beijing has decreased dramatically from 1990 to 2017. Hepatitis A vaccine plays an important role in protecting individuals under 20 years old. A higher proportion of adults will be susceptible to hepatitis A virus due to the decay of antibodies as they grow up from childhood to adulthood, which may result in possible outbreak of hepatitis A.

Electrically assisted manufacturing (EAM) is a promising and rapidly developing metal processing method. The power supply is a key sub-system for EAM, which needs to be designed properly. This paper proposes the model-based design of a low-voltage high-current pulse power supply used for EAM based on converter-level electro-thermal modeling. The thermal stress of key components is obtained by converter-level finite element simulations. A simplified thermal modeling method is proposed to reduce the computation burden of the FEM simulation to obtain the dynamic thermal profile under pulse current operation. The impact of the duration of the current pulse on the maximum temperature and temperature variations of MOSFETs is investigated based on the thermal model. A case study of a 10V/500A pulse power supply is presented to demonstrate the theoretical analyses and verification. The outcomes contribute to the design optimization and virtual prototyping of pulse power supplies for EAM applications.

To learn discriminative features, hyperspectral image (HSI), containing 3-D cube data, is a preferable means of capturing multi-head self-attention from both spatial and spectral domains if the burden in model optimization and computation is low. In this paper, we design a dual multi-head contextual self-attention (DMuCA) network for HSI classification with the fewest possible parameters and lower computation costs. To effectively capture rich contextual dependencies from both domains, we decouple the spatial and spectral contextual attention into two sub-blocks, SaMCA and SeMCA, where depth-wise convolution is employed to contextualize the input keys in the pure dimension. Thereafter, multi-head local attentions are implemented as group processing when the keys are alternately concatenated with the queries. In particular, in the SeMCA block, we group the spatial pixels by evenly sampling and create multi-head channel attention on each sampling set, to reduce the number of the training parameters and avoid the storage increase. In addition, the static contextual keys are fused with the dynamic attentional features in each block to strengthen the capacity of the model in data representation. Finally, the decoupled sub-blocks are weighted and summed together for 3-D attention perception of HSI. The DMuCA module is then plugged into a ResNet to perform HSI classification. Extensive experiments demonstrate that our proposed DMuCA achieves excellent results over several state-of-the-art attention mechanisms with the same backbone.

Influences of chemical dilution and the number of weld layers on residual stresses in a multi-pass low-transformation-temperature (LTT) weld were investigated by finite element modelling and neutron diffraction. A coupled thermal-metallurgical-mechanical (TMM) model that took into account the chemical dilution effect was developed to simulate the complex LTT welding phenomena. The model was strictly validated by comparing the predictions with experimental measurements and the results found good agreement between them. The results showed that a transformation strain caused by LTT martensitic transformation introduced large compressive residual stresses (−500 MPa) into the weld zone and the quantities were closely related to the chemical dilution between welding layers. Simulation results revealed that the chemical dilution helped mitigate the large localized tensile residual stresses by modulating the phase transformation process and a single LTT layer was sufficient to generate high compression near the weld surface (~2.5 mm), which suggests a great importance for repair welding. Keywords: Finite element analysis, Thermal-metallurgical-mechanical model, Martensitic transformation, Residual stress, Chemical dilution, Multi-pass weld

According to the two-dimensional aperture principle, directivity and gain are expected to be proportional to the aperture area of an antenna or antenna array. Our simulations and measurements of an end-fire radiation antenna array overturn this conventional wisdom. The gain of an end-fire antenna array is found to be much higher than that calculated by the two-dimensional aperture principle. We introduce a length component into the two-dimensional aperture principle and propose a three-dimensional aperture principle. A four-element end-fire antenna array is designed and measured to verify the correctness of this new principle. The three-dimensional aperture principle opens a pathway to novel types of aperture utilization.

Qingda granules (QDG) are derived from QingXuanJiangYa Decoction (QXJYD) a traditional Chinese medication that has been used to treat hypertension for more than 60 years. QXJYD has been shown to be effective in rat models of hypertension. However, the effects of QDG on hypertension remain largely unknown. In the current study, baicalin was identified as one of the main components of QDG using Ultra Performance Liquid Chromatography (UPLC) analysis. We investigated the effects of QDG on blood pressure, cardiac remodeling, and cardiac inflammation. QDG (0.8 g/kg/day) treatment attenuated the elevated blood pressure in spontaneously hypertensive rats (SHRs). Moreover, QDG treatment reduced the degree of myocardial fiber disarray, degeneration and necrosis of myocardial cells, expression of ANP and BNP, as well as collagen content of SHRs. Moreover, we further assessed the effect of QDG treatment on cardiac inflammation and found that QDG treatment reduced CD68 protein expression, decreased levels of IL-6 and TNF-α in both serum and cardiac tissues, as well as suppressed activation of NF-κB pathway in cardiac tissues of SHRs. Differential expressed metabolites (DEMs) analysis identified 41 increased and 51 decreased metabolites in the cardiac tissues of SHRs after QDG treatment. In summary, QDG treatment of SHRs attenuated the elevated blood pressure and ameliorated cardiac remodeling and inflammation, in part, through suppression of NF-κB pathway and DEMs, which provide a basis for other therapeutic uses of this TCM.

Abstract Along with the increasing penetration of distributed generation with voltage-source converters (VSCs), there are extensive concerns over the potential virtual rotor angle stability, which is characterized by oscillations of power and frequency during the dynamic process of synchronization in the grid. Several control strategies have been developed for VSCs to emulate rotating inertia as well as damping of oscillations. This paper classifies these strategies and provides a small-signal modeling framework including all kinds of VSCs in different applications for virtual rotor angle stability. A unified perspective based on the famous Phillips–Heffron model is established for various VSCs. Thus, the concepts of equivalent inertia and the synchronizing and damping coefficients in different VSCs are highlighted, based on the similarities with the synchronous generator (SG) system in both physical mechanisms and mathematical models. It revealed the potentiality of various VSCs to achieve equivalence with the SG. This study helps promote the unity of VSCs and traditional SGs in both theories and methods for analyzing the dynamic behavior and enhancing the stability. Finally, future research needs and new perspectives are addressed.

Hyperspectral images (HSIs), acquired as a 3D data set, contain spectral and spatial information that is important for ground–object recognition. A 3D convolutional neural network (3DCNN) could therefore be more suitable than a 2D one for extracting multiscale neighborhood information in the spectral and spatial domains simultaneously, if it is not restrained by mass parameters and computation cost. In this paper, we propose a novel lightweight multilevel feature fusion network (LMFN) that can achieve satisfactory HSI classification with fewer parameters and a lower computational burden. The LMFN decouples spectral–spatial feature extraction into two modules: point-wise 3D convolution to learn correlations between adjacent bands with no spatial perception, and depth-wise convolution to obtain local texture features while the spectral receptive field remains unchanged. Then, a target-guided fusion mechanism (TFM) is introduced to achieve multilevel spectral–spatial feature fusion between the two modules. More specifically, multiscale spectral features are endowed with spatial long-range dependency, which is quantified by central target pixel-guided similarity measurement. Subsequently, the results obtained from shallow to deep layers are added, respectively, to the spatial modules, in an orderly manner. The TFM block can enhance adjacent spectral correction and focus on pixels that actively boost the target classification accuracy, while performing multiscale feature fusion. Experimental results across three benchmark HSI data sets indicate that our proposed LMFN has competitive advantages, in terms of both classification accuracy and lightweight deep network architecture engineering. More importantly, compared to state-of-the-art methods, the LMFN presents better robustness and generalization.

This paper proposes an intelligent seamless transition controller for smooth transition between grid-connected (GC) and standalone modes of distributed generation (DG) units in the grid. The development of this seamless controller contributes to two main processes in the transition modes: the synchronization process and an islanding process. For the synchronization process, the stationary reference frame phase-locked loop (SRF-PLL) associated with the voltage source inverter (VSI) is modified using the frequency, voltage deviation, and phase angle information. Furthermore, the islanding process is classified as intentional and unintentional islanding scenarios for achieving efficient transition control. Here, the intentional islanding process is achieved with the information that is available in the system due to the planned disconnection. For the unintentional islanding process, a fuzzy inference system (FIS) is used to modify the conventional droop control using the information of change in active power, voltage, and frequency. To identify the action of the proposed approach during the transition process, numerical simulations are conducted with the hardware-in-loop (HIL) simulator by developing a 10kWp three-phase grid-connected DG system. The results identified the efficient control of the VSI for both islanding and grid connection processes. In the islanding conditions, the proposed controller provides advantage with less detection and disconnection time, and during synchronization, it instantly minimizes the phase-angle deviation to achieve efficient control.

The protective role of alkaloids from Nelumbinis Plumula (AFNP) on the aorta during hypertension is not yet fully understood. We hypothesize that AFNP exerts protective effects against Ang II-induced hypertension by mediating RhoA/ROCK pathway and phenotypic switching during hypertension. In the present study, we evaluated the effect of AFNP on angiotensin II (Ang II)-induced actin cytoskeleton reorganization and aorta remodeling, as well as the involvement of RhoA/Rho-associated coiled kinase (ROCK) pathway in protecting against hypertension. We used rat aortic tissues to investigate the vasodilatation effect of AFNP on Ang II-induced constriction. AFNP was shown to significantly relax the endothelium-intact arteries induced by Ang II. We further investigated the vasodilation effect of AFNP in endothelium denuded arteries, which showed that the action of AFNP was endothelial independent. Male SHR rats were treated with saline or AFNP and morphological changes were examined following 8 weeks. AFNP treatment normalized the effects of hypertension in SHRs. HE staining showed that AFNP treatment improved the tunica media and wall thickness and ratio of MT/LD and MA/LA. Western blotting showed that AFNP treatment markedly decreased the Ang II-induced expression of collagen I and increased α-SMA in aorta. Furthermore, MTT assay showed that AFNP inhibited the proliferation of Ang II treated VSMCs in a concentration-dependent manner. AFNP treatment also ameliorated F-actin cytoskeleton remodeling in Ang II treated VSMCs, as visualized under fluorescence microscopy. Western blot analysis showed that RhoA transposition and ROCK activation and phosphorylation of MYPT1 was increased following Ang II treatment but were inhibited by AFNP treatment, showing that the cardio-protective effect of AFNP is likely mediated by the RhoA/ROCK signaling pathway. The anti-hypertension and aortic protection effects of AFNP are due to non-endothelial dependent inhibition of the VSMC cytoskeleton remodeling and regulation of RhoA/ROCK pathway.

Interleaved power factor correction (PFC) is widely used circuit topology due to good efficiency and power density for single-switch boost PFC. As the differential mode (DM) electromagnetic interference (EMI) noise magnitude depends upon the input current ripple, this research details a comprehensive study of DM EMI filter design for interleaved boost PFC with the aim of minimizing the component size. It is also demonstrated that the different numbers of interleaved stages and switching frequency influence the filter attenuation requirement and, thus, the EMI filter size. First, an analytical model is derived on the basis of the Norton equivalent circuit model for the differential mode noises of interleaved boost PFC within the frequency range of 9–500 kHz. The derived model can help identify the proper phase shifting among the interleaved boost converters in order to minimize the considered differential mode noises at the filter design frequency. So, a novel phase-shift method is developed to get a minimized attenuation required by a filter in Band B. Further, a volume optimization of the required DM filter was introduced based on the calculated filter attenuation and volumetric component parameters. Based on the obtained results, unconventional and conventional phase shifts have demonstrated a good performance in decreasing the EMI filter volume in Band B and Band A, respectively. A 2-kW interleaved PFC case study is presented to verify the theoretical analyses and the impact of phase-shifting on EMI filter size.

A high end-fire gain, low-profile surface wave antenna with capacitive feed structure is presented in this paper. The proposed dielectric-metal surface wave antenna is composed of a dielectric slab that is mounted on a metal carrier and a low-profile feed structure. The feed structure is composed of a monopole radiation pin that is loaded with a circular metal plate and a grounding pin. The profile height of the antenna is only one-tenth of the operating wavelength. With a good end-fire performance and low profile, the antenna is very suitable to be conformally mounted on the surface of flight vehicles. The proposed antenna was designed and manufactured at the center frequency of 6 GHz. Measured results demonstrated that the proposed antenna had a bandwidth of 7.33%, ranging from 5.89 to 6.33 GHz, and the antenna reached a high gain of 9.76 dBi with a length of 122.96 mm (2.45 λ) in the end-fire direction.

In power electronic converters, reliability of DC-link capacitors is one of the critical issues. The estimation of their health status as an application of condition monitoring have been an attractive subject for industrial field and hence for the academic research filed as well. More reliable solutions are required to be adopted by the industry applications in which usage of extra hardware, increased cost, and low estimation accuracy are the main challenges. Therefore, development of new condition monitoring methods based on software solutions could be the new era that covers the aforementioned challenges. A capacitance estimation method based on Artificial Neural Network (ANN) algorithm is therefore proposed in this paper. The implemented ANN estimated the capacitance of the DC-link capacitor in a back-toback converter. Analysis of the error of the capacitance estimation is also given. The presented method enables a pure software based approach with high parameter estimation accuracy.

Electroslag remelting (ESR) is an effective method for removing nonmetallic inclusions from steels or alloys. The main stage of inclusion removal during ESR is the aggregation of liquid metal film (LMF) to form droplets at the consumable electrode tip. In this study, a lab-level ESR experiment was carried out. The number and size of inclusions at the characteristic position of the electrode were quantitatively counted. The number of inclusions in the center position of LMF were larger than that in other regions. To elucidate these phenomena, a two-dimensional mathematical model was established to study the migration of inclusions in LMF. The results indicate that due to the large velocity gradient in LMF, the Saffman force is strong enough to offset the buoyant force and drag the inclusions toward the slag/LMF interface (SFI), where the inclusions will be dissolved in the SFI region by the molten slag. This study demonstrates that the Saffman force plays a key role in the removal of nonmetallic inclusions in LMF during the ESR process.

Submodule (SM) capacitors are key elements in the modular multilevel converter (MMC), the design of which influences the entire system performance. In practical cases, SM capacitor sizing must consider the abnormal system operation (e.g., grid faults). In order to find a clear design boundary for SM capacitors, a practical capacitor sizing method is presented for the first time in this paper, considering the grid-fault-ride-through operation of the MMC, impact of MMC control system, and aging mechanism of capacitors. The SM capacitor rated voltage, capacitance, ESR, thermal resistance, and lifetime can be decided to ensure reliable operations of the MMC during grid faults. The effectiveness of the proposed method has been verified through experimental tests on a down-scale MMC system.

Indigenous cattle in Sichuan Province, southwestern China, provide abundant genetic resources. However, their genetic diversity and population structure remain largely unknown, especially on the genome-wide scale. In the present study, we successfully employed the restriction site-associated DNA sequencing approach (RADseq) to explore genome-wide SNPs among six breeds of Sichuan cattle. A total of 238,725 high-confidence SNPs were finally obtained with a mean distance of 11,140 bp between two adjacent sites, and 43.4% were revealed to be novel in comparison with a public reference database of genetic variants. The mean nucleotide diversity and polymorphism information content (PIC) among all six breeds were 0.1878 and 0.1555, respectively. Pingwu and Ganzi cattle showed the highest and lowest genetic diversity, respectively. The inter-breed comparisons revealed that Ganzi and Ebian cattle were obviously separate from the others. Our reference set of genome-wide SNPs specific to indigenous cattle in Sichuan is the first of its kind. Moreover, our set can be used to investigate the genetic diversity and population structure and for genome-wide association studies.

The effects of sulfur trioxide micro-thermal explosion (STEX) and enzyme loading on reducing sugars conversion of STEX-treated rice straw and enzymatic hydrolysates were researched. Important process parameters in the pretreatment of biomass were identified by a Plackett-Burman design, and parameters with significant effects were optimized using a Box-Behnken design (BBD) and response surface methodology (RSM). The optimal conditions were a temperature of 80 °C and a treatment time of 30 min when only single factors were considered. Meanwhile, glucose and xylose were primary components in the enzymatic hydrolysates. Subsequently, STEX time, liquid-solid ratio, and soaking temperature were the main factors governing the enzymatic saccharification of rice straw. The optimum pretreatment conditions were STEX time 23.3 min, liquid-solid ratio 13.3 (V/m), and soaking temperature 62.2 °C. The chemical composition analysis of straw further demonstrated that STEX collaborative dilute lye pretreatment could remove lignin and hemicellulose.

Due to the small volume and high efficiency, transformerless inverters have gained much popularity in grid-connected PV applications, where minimizing leakage current injection is mandatory. This can be achieved by either modifying the modulation schemes or adding extra power switching devices, resulting in an uneven distribution of the power losses on the switching devices. Consequently, the device thermal loading is redistributed and thus may alter the entire inverter reliability performance, especially under a long-term operation. In this consideration, this paper assesses the device reliability of three transformerless inverters under a yearly mission profile (i.e., solar irradiance and ambient temperature). The mission profile is translated to device thermal loading, which is used for lifetime prediction. Comparison results reveal the lifetime mismatches among the power switching devices operating under the same condition, which offers new thoughts for a robust design and a reliable operation of grid-connected transformerless PV inverters with high efficiency.

Background: Upper limb motor impairment is a common consequence of stroke, and the effectiveness and underlying mechanisms of rehabilitation therapy for improving upper limb function remain uncertain. Functional nearinfrared spectroscopy, a reliable wearable neuroimaging technique, holds promise for investigating brain activity during functional tasks. This study aims to explore the synchronous oxygenation characteristics of the central cortex and upper-limb flexors during a grasping task and investigate the rehabilitation mechanisms of upper limb motor function in individuals with stroke. Methods: Participants with stroke who demonstrate the ability to grasp and lift cubic wood blocks of different sizes (2.5cm3, 5cm3, and 10cm3) using their affected hand will be divided into three groups: A, B, and C. Each group will consist of twenty stroke patients, resulting in a total of sixty participants with stroke. Additionally, twenty matched healthy subjects will be enrolled as a control group. Comprehensive assessments will be conducted before and after the intervention, including blood oxygen parameter monitoring of the cerebral cortex and upper limb flexors using fNIRS during the grasping task. Other assessments will include MyotonPRO, the Modified Ashworth Scale, the upper extremity section of the Fugl-Meyer Assessment, the Action Research Arm Test, and the Modified Barthel Index. The study will be undertaken between January 2024 and September 2025. Conclusions: The results of this trial will provide an in-depth understanding of the Characteristics of central cortex and upper-limb flexors synchronous oxygenation during grasping task and how it may relate to the rehabilitation mechanism of upper limb motor function in people with stroke. [ABSTRACT FROM AUTHOR]