27 results on '"Hideaki Ikeda"'
Search Results
2. Cryopreservation of Primarily Isolated Porcine Hepatocytes with UW Solution
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Takemi Kunieda, Masanobu Maruyama, Teru Okitsu, Norikuni Shibata, Michihiko Takesue, Toshinori Totsugawa, Yoshikazu Kosaka, Takashi Arata, Kazuya Kobayashi, Hideaki Ikeda, Mizuko Oshita, Shuhei Nakaji, Kenji Ohmoto, Shinichiro Yamamoto, Yuzuru Kurabayashi, Makoto Kodama, Noriaki Tanaka, and Naoya Kobayashi M.D., PH.D.
- Subjects
Medicine - Abstract
Development of liver-targeted cell therapies, such as hepatocyte transplantation and bioartificial livers, requires a large amount of functional hepatocytes as needed. To achieve this development, establishing an excellent cryopreservation method of hepatocytes is an extremely important issue. Therefore, we performed a comparative review of cryoprotective effects of various cryopreservation solutions using primarily isolated porcine hepatocytes. Porcine hepatocytes were isolated with a four-step dispase and collagenase perfusion method. The obtained hepatocytes with the initial viabilities of 76%, 84%, and 96% were assigned to the following four groups for cryopreservation at −80°C: Dulbecco's modified Eagle's medium (DMEM) + 10% fetal bovine serum (FBS) + 12% dimethyl sulfoxide (DMSO) (group A), University of Wisconsin (UW) solution + 12% DMSO (group B), Cell Banker 1 (group C), and Cell Banker 2 (group D). The hepatocytes in each group were thawed at 3 days, 10 days, and 5 months of cryopreservation and subjected to comparative analyses, including viability, plating efficiency, LDH release, ammonia removal test, and lentiviral gene transfer. These parameters were the most favorable in the hepatocytes cryopreserved with UW solution. Approximately 5% of thawed cryopreserved porcine hepatocytes expressed LacZ activity after lentiviral transduction. Intrasplenic transplantation of UW solution-cryopreserved hepatocytes improved the survival of rats treated with D-galactosamine. UW solution maintained the functions of cryopreserved porcine hepatocytes.
- Published
- 2003
- Full Text
- View/download PDF
3. Maintenance of Cold-Preserved Porcine Hepatocyte Function with UW Solution and Ascorbic Acid-2 Glucoside
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Michihiko Takesue, Masanobu Maruyama, Norikuni Shibata, Takemi Kunieda, Teru Okitsu, Masakiyo Sakaguchi, Toshinori Totsugawa, Yoshikazu Kosaka, Akira Arata, Hideaki Ikeda, Junji Matsuoka, Toshie Oyama, Makoto Kodama, Kenji Ohmoto, Shinichiro Yamamoto, Yuzuru Kurabayashi, Itaru Yamamoto, Noriaki Tanaka, and Naoya Kobayashi M.D., PH.D.
- Subjects
Medicine - Abstract
Normal human hepatocytes are an ideal source of liver-targeted cell therapies, such as hepatocyte transplantation and bioartificial livers, but availability of human donor livers for liver cell isolation is severely limited. To effectively utilize scarce donor organs for cell therapies, it is of extreme importance to establish an efficient isolation technique and an effective cold preservation solution for transportation of isolated cells. A lateral segment of the liver was surgically resected from pigs weighing 10 kg and a four-step collagenase and dispase digestion was conducted. Isolated hepatocytes were subjected to 8-h cold storage on ice. The following preservation solutions were tested: 1) University of Wisconsin (UW) solution, 2) UW with 100 μg/ml of ascorbic acid-2 glucoside (AA2G), 3) 100% fetal bovine serum (FBS), and 4) Dulbecco's modified Eagle's medium (DMEM) supplemented with 100% FBS. The mean viability of porcine hepatocytes was 95.5 ± 2.5% when isolated in three independent experiments. Viability, plating efficiency, membrane stability, and ammonia metabolic capacity of cold-preserved hepatocytes were significantly better maintained by the use of UW solution. When AA2G (100 μg/ml) was combined with UW solution, such parameters were further improved. It was explained by inhibition of caspase-3 activation and retention of ATP at high levels of hepatocytes preserved with UW solution containing AA2G. The present work demonstrates that a combination of UW solution with AA2G (100 μg/ml) would be a useful cold preservation means for the development of cell therapies.
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- 2003
- Full Text
- View/download PDF
4. 腹腔鏡内視鏡合同手術(Laparoscopy and Endoscopy Cooperative Surgery;LECS) を施行したS状結腸膀胱瘻の1例
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Yutaka Yamashita, Takushi Kurashige, Masahiro Oishi, Daisuke Nishikawa, Yuma Tani, Hideaki Ikeda, Kenji Mizuno, Masahito Kodera, Hiroshi Kato, Shunji Hayata, Masao Yamamura, and Yasuhiro Nishiyama
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medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,General surgery ,Fistula ,腹腔鏡内視鏡合同手術(laparoscopic and endoscopic cooperative surgery(LECS)) ,General Medicine ,Endoscopic submucosal dissection ,Diverticulitis ,medicine.disease ,Cystectomy ,Sigmoidectomy ,結腸膀胱瘻(sigmoidovesical fistula) ,Invasive surgery ,medicine ,Partial colectomy ,sense organs ,business ,Abdominal surgery - Abstract
We performed laparoscopic and endoscopic cooperative surgery (LECS) for partial colectomy with partial cystectomy in an 80-year-old woman with sigmoidovesical fistula secondary to sigmoid diverticulitis. LECS was designed for local resection of the stomach for gastric submucosal tumors using the endoscopic submucosal dissection (ESD) technique. While conventional open abdominal surgery is very invasive, LECS has enabled surgeons to perform minimally invasive surgery. Although there have been no reported cases in which the bladder was laparoscopically resected after sigmoidovesical fistula division, we were able to safely resect the fistula and preserve the urethral opening by performing laparoscopic and cystoscopic cooperative surgery. There are no previously reported cases in which LECS was performed for partial sigmoidectomy and partial cystectomy ; therefore, we report this as a valuable case, with a review of the literature. We hope that further studies involving more patients will lead to the establishment of this procedure.
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- 2015
5. A Case of Giant Bulla with Spontaneous Pneumothorax which Required Lobectomy of the Lung
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Masahiro Oishi, Hiroshi Katoh, Yutaka Yamashita, Masao Yamamura, Hideaki Ikeda, and Kenji Mizuno
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Pathology ,medicine.medical_specialty ,Lung ,medicine.anatomical_structure ,Pneumothorax ,business.industry ,Giant bulla ,medicine ,medicine.disease ,business ,Surgery - Published
- 2015
6. A Case of Double Cancer Involving the Thyroid and Lung in which cN3 Lung Cancer Could be Denied Preoperatively
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Kenji Mizuno, Hideaki Ikeda, Masahiro Oishi, Yutaka Yamashita, Masao Yamamura, and Hiroshi Katoh
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Oncology ,medicine.medical_specialty ,Lung ,business.industry ,Thyroid ,Cancer ,medicine.disease ,medicine.anatomical_structure ,Internal medicine ,medicine ,Radiology ,Double cancer ,business ,Lung cancer - Published
- 2015
7. Laparoscopic total colectomy with lymph node dissection for familial adenomatous polyposis with multiple colorectal cancers
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Hideaki Ikeda, Kenji Mizuno, Masahito Kodera, Hiroshi Kato, Masahiro Oishi, Masao Yamamura, and Yutaka Yamashita
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medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Rectum ,Sigmoid colon ,medicine.disease ,Inferior mesenteric artery ,Surgery ,Familial adenomatous polyposis ,medicine.anatomical_structure ,medicine.artery ,medicine ,Superior mesenteric artery ,Laparoscopy ,business ,Lymph node ,Abdominal surgery - Abstract
A 49-year-old Japanese man visited our hospital with chief complaints of difficulty with and bleeding during defecation. After a detailed examination, he was diagnosed with familial adenomatous polyposis (FAP) with multiple (five) colorectal cancers. The tumors were located in the right-sided, left-sided, and sigmoid colon, and the lower rectum. Regional lymph node involvement was observed, but no metastasis to other organs was detected. We, therefore, performed a laparoscopic total colectomy with superior mesenteric artery (SMA) and inferior mesenteric artery (IMA) lymph node dissection. We were able to perform minimally invasive and cosmetically acceptable surgery using laparoscopy instead of highly-invasive open abdominal surgery. Our search of the literature revealed no reported cases of laparoscopic total colectomy with lymph node dissection for FAP with multiple colorectal cancers, making the present case the first to be reported in the literature.
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- 2015
8. Improvement of mechanical durability on organic TFT with printed electrodes prepared from nanoparticle ink
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Daisuke Kumaki, Kenjiro Fukuda, Akifumi Kosakai, Shizuo Tokito, Hideaki Ikeda, and Tomohito Sekine
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chemistry.chemical_classification ,Materials science ,Scanning electron microscope ,General Physics and Astronomy ,Sintering ,Nanoparticle ,Surfaces and Interfaces ,General Chemistry ,Polymer ,Condensed Matter Physics ,Surfaces, Coatings and Films ,chemistry ,Thin-film transistor ,Electrode ,Composite material ,Glass transition ,Layer (electronics) - Abstract
We investigated a mechanism of improving adhesiveness depending on sintering temperature for printed silver (Ag) electrodes prepared from nanoparticle ink on an insulating polymer layer as the under layer. The adhesion strength significantly improved by sintering the Ag electrodes at a temperature above the glass transition temperature ( T g ) of the polymer layer. In the sample with improved adhesiveness, the interfacial fusion between the printed Ag electrodes and polymer layer was observed in a cross sectional scanning electron microscope image. Based on this mechanism, we have successfully demonstrated the improvement in mechanical durability of organic thin-film transistors (TFTs) with printed Ag electrodes. The degradation of the organic TFTs after applying bending stress was significantly suppressed by interfacial fusion.
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- 2014
9. A case of asymptomatic right-sided adult Bochdalek hernia presented incidentally with rectal tumor
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Kenji Mizuno, Masao Yamamura, Kazunori Suzuki, Masahiro Oishi, Hideaki Ikeda, Yutaka Yamashita, Masahito Kodera, and Hiroshi Kato
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Laparoscopic surgery ,medicine.medical_specialty ,business.industry ,General surgery ,medicine.medical_treatment ,medicine.disease ,Asymptomatic ,digestive system diseases ,Surgery ,Bochdalek hernia ,surgical procedures, operative ,成人Bochdalek孔ヘルニア(adult Bochdalek hernia) ,Medicine ,腹腔鏡下手術(laparoscopic surgery) ,medicine.symptom ,business - Abstract
We encountered a patient with an adult Bochdalek hernia discovered asymptomatically. A 77-year-old Japanese woman visited a local clinic with chief complaints of melena and difficulty in defecation. Based on the results of the detailed examination in our hospital, she was diagnosed with a rectal gastrointestinal stromal tumor (GIST) with a concurrent asymptomatic adult right-sided Bochdalek hernia. Because the tumor was large, laparoscopic abdominoperineal rectal amputation was performed after systemic imatinib therapy. During the surgery, we found a right diaphragmatic defect more than 13cm in long dia., through which the right hepatic lobe, colon, and greater omentum had prolapsed into the right thoracic cavity. No visceral adhesions were noted. No hernia sac was observed. Adult Bochdalek hernia is a relatively rare condition, and only three (incidentally discovered) cases of asymptomatic Bochdalek hernia, including the present case, have been reported in Japan. Here we provide a case report for the patient, who was followed-up without hernia surgery, plus a review of the literature.
- Published
- 2014
10. A CASE OF INTRACTABLE SUBAREOLAR ABSCESS THAT WAS CURED BY EXCISION OF FISTULA WITH A THREAD GUIDE
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Masahiro Ohishi, Hideaki Ikeda, Yutaka Yamashita, and Masahito Kodera
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Subareolar abscess ,medicine.medical_specialty ,business.industry ,Fistula ,medicine ,medicine.disease ,business ,Surgery - Abstract
症例は25歳,女性,未婚.2004年12月,左乳輪内側にしこり生じるも,自然消失.しかし,生理とともに再燃した.近医を受診したが,様子をみようといわれ不安になり,2月当院初診となった.触診,エコーで左乳輪下に膿瘍と思われるspaceあり,穿刺にて排膿なく,細胞診でも悪性所見なし.乳頭陥凹を認めず.発赤も軽く,切開せず経過観察となった.3月始め生理とともに発赤増悪し再来.エコーでspace拡大あり.切開排膿施行した.その後治癒と再燃を繰り返した.7月乳頭より圧迫にて排膿あり.3-0ナイロン糸を瘻孔開口部から挿入すると,乳頭より出てきた.そのまま糸をループ状に結紮,留置した.9月全身麻酔下に手術施行.通したナイロン糸をガイドに可及的に瘻孔を切除,乳頭内の責任乳管の開放と掻爬を行った.その後3カ月経過するも再燃を認めていない.
- Published
- 2009
11. Noise-robust recognition of wide-field motion direction and the underlying neural mechanisms in Drosophila melanogaster
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Toru Aonishi, Yoshinori Suzuki, Takuya Miyamoto, Takako Morimoto, Hideaki Ikeda, Hiroyoshi Miyakawa, and Yoichi Seki
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Patch-Clamp Techniques ,Motion Perception ,Environment ,Article ,Sensation ,Animals ,Computer vision ,Computer Simulation ,Motion perception ,Patch clamp ,Motion detector ,Neurons ,Multidisciplinary ,biology ,Spatial filter ,Behavior, Animal ,Noise (signal processing) ,business.industry ,Brain ,biology.organism_classification ,Drosophila melanogaster ,Flight, Animal ,Optomotor response ,Artificial intelligence ,Biological system ,business ,Noise - Abstract
Appropriate and robust behavioral control in a noisy environment is important for the survival of most organisms. Understanding such robust behavioral control has been an attractive subject in neuroscience research. Here, we investigated the processing of wide-field motion with random dot noise at both the behavioral and neuronal level in Drosophila melanogaster. We measured the head yaw optomotor response (OMR) and the activity of motion-sensitive neurons, horizontal system (HS) cells, with in vivo whole-cell patch clamp recordings at various levels of noise intensity. We found that flies had a robust sensation of motion direction under noisy conditions, while membrane potential changes of HS cells were not correlated with behavioral responses. By applying signal classification theory to the distributions of HS cell responses, however, we found that motion direction under noise can be clearly discriminated by HS cells and that this discrimination performance was quantitatively similar to that of OMR. Furthermore, we successfully reproduced HS cell activity in response to noisy motion stimuli with a local motion detector model including a spatial filter and threshold function. This study provides evidence for the physiological basis of noise-robust behavior in a tiny insect brain.
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- 2015
- Full Text
- View/download PDF
12. Maintenance of glucose-sensitive insulin secretion of cryopreserved human islets with University of Wisconsin solution and ascorbic acid-2 glucoside
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Junji Matsuoka, Yoshikazu Kosaka, Teru Okitsu, Naoya Kobayashi, Chen Yong, Jonathan R. T. Lakey, Itaru Yamamoto, Kazuya Kobayashi, Takashi Arata, Takuya Fukazawa, Michiki Narushima, Hideaki Ikeda, and Noriaki Tanaka
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endocrine system ,Adenosine ,endocrine system diseases ,University of Wisconsin solution ,Cell Survival ,Cell Transplantation ,Allopurinol ,Organ Preservation Solutions ,Transplantation, Heterologous ,Biomedical Engineering ,Medicine (miscellaneous) ,Heterologous ,Mice, Nude ,Bioengineering ,Ascorbic Acid ,Biology ,cryopreservation ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Cryopreservation ,Biomaterials ,Andrology ,Islets of Langerhans ,Mice ,Raffinose ,In vivo ,Insulin Secretion ,Animals ,Humans ,Insulin ,Viaspan ,stimulation index ,Cells, Cultured ,Proinsulin ,islets ,geography ,geography.geographical_feature_category ,ascorbic acid-2 glucoside ,Gene Transfer Techniques ,General Medicine ,DNA ,Islet ,Glutathione ,Transplantation ,Disease Models, Animal ,Biochemistry ,RNA ,Tissue Preservation ,Fetal bovine serum - Abstract
Normal human islet cells are an ideal source for pancreas-targeted cell therapies, but the availability of human donor pancreata for islet isolation is severely limited. To effectively utilize such scarce donor organs for cell therapies, it is crucial to develop an excellent isolation, effective cryopreservation, and efficient gene transfer techniques for the transportation of isolated cells. In the present study, we investigate the effect of University of Wisconsin (UW) solution and ascorbic acid-2 glucoside (AA2G) on the cryopreservation of human islets. We also evaluate the gene transfer efficiency of a lentiviral vector expressing the E. coli LacZ gene, Lt-NLS/LacZ, in human islets. Human islets were isolated with a standard digestion method at the University of Alberta. Isolated islets were transported to Japan for 40 h and then subjected to cryopreservation experiments. The following preservation solutions were tested: UW solution with 100 micro g/mL of AA2G, UW solution, 100% fetal bovine serum (FBS), and CMRL supplemented with 10% FBS. Following three months of cryopreservation, the islets were thawed and analyzed for viability, glucose-sensitive insulin secretion, proinsulin gene expression profile, and in vivo engraftment. The islets were also subjected to monolayer formation with 804G-cell-line-derived extracellular matrix (ECM), followed by Lt-NLS/LacZ transduction. The viability, morphology, glucose-sensitive insulin secretion, proinsulin gene expression, and monolayer formation efficiency of the thawed cryopreserved islets are significantly better maintained by the use of UW solution. When AA2G (100 microg/mL) is combined with UW, such parameters are further improved. The adequate engraftment of UW + AA2G-cryopreserved human islets is achieved in the liver of nude mice. Efficient Lt-NLS/LacZ transduction is identified in monolayered islets cryopreserved with UW solution with AA2G. The present work demonstrates that the combination of UW solution with AA2G (100 microg/mL) would be a useful cryopreservation means for human islets. Human islets monolayer-cultured with 804G-derived ECM are efficiently transduced with a lentiviral vector Lt-NLS/LacZ.
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- 2004
13. Cryopreservation of Primarily Isolated Porcine Hepatocytes with UW Solution
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Teru Okitsu, Hideaki Ikeda, Noriaki Tanaka, Shinichiro Yamamoto, N Kobayashi, Masanobu Maruyama, Toshinori Totsugawa, Takemi Kunieda, Kazuya Kobayashi, Makoto Kodama, Takashi Arata, Norikuni Shibata, Mizuko Oshita, Shuhei Nakaji, Kenji Ohmoto, Yoshikazu Kosaka, Yuzuru Kurabayashi, and Michihiko Takesue
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Male ,0301 basic medicine ,Adenosine ,Plating efficiency ,Cell Survival ,Cell Transplantation ,Allopurinol ,Genetic Vectors ,Organ Preservation Solutions ,Sus scrofa ,Transplantation, Heterologous ,Cell Culture Techniques ,Biomedical Engineering ,lcsh:Medicine ,Cell Separation ,Cryopreservation ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,Cryoprotective Agents ,Raffinose ,0302 clinical medicine ,Ammonia ,Transduction, Genetic ,Dispase ,medicine ,Animals ,Insulin ,Viaspan ,Rats, Wistar ,Transplantation ,L-Lactate Dehydrogenase ,Chemistry ,Dimethyl sulfoxide ,Liver Diseases ,lcsh:R ,Cell Biology ,Glutathione ,Liver Transplantation ,Rats ,030104 developmental biology ,medicine.anatomical_structure ,Lac Operon ,Hepatocyte ,Hepatocytes ,030217 neurology & neurosurgery ,Fetal bovine serum - Abstract
Development of liver-targeted cell therapies, such as hepatocyte transplantation and bioartificial livers, requires a large amount of functional hepatocytes as needed. To achieve this development, establishing an excellent cryopreservation method of hepatocytes is an extremely important issue. Therefore, we performed a comparative review of cryoprotective effects of various cryopreservation solutions using primarily isolated porcine hepatocytes. Porcine hepatocytes were isolated with a four-step dispase and collagenase perfusion method. The obtained hepatocytes with the initial viabilities of 76%, 84%, and 96% were assigned to the following four groups for cryopreservation at −80°C: Dulbecco's modified Eagle's medium (DMEM) + 10% fetal bovine serum (FBS) + 12% dimethyl sulfoxide (DMSO) (group A), University of Wisconsin (UW) solution + 12% DMSO (group B), Cell Banker 1 (group C), and Cell Banker 2 (group D). The hepatocytes in each group were thawed at 3 days, 10 days, and 5 months of cryopreservation and subjected to comparative analyses, including viability, plating efficiency, LDH release, ammonia removal test, and lentiviral gene transfer. These parameters were the most favorable in the hepatocytes cryopreserved with UW solution. Approximately 5% of thawed cryopreserved porcine hepatocytes expressed LacZ activity after lentiviral transduction. Intrasplenic transplantation of UW solution-cryopreserved hepatocytes improved the survival of rats treated with D-galactosamine. UW solution maintained the functions of cryopreserved porcine hepatocytes.
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- 2003
14. Contribution of Reactor Physics in Past and Future ; Is Reactor Physics Useful?
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Koichi Sakurada, Toshikazu Takeda, Junichi Koyama, Katsuhisa Sengoku, Hiroshi Sekimoto, Seiji Shiroya, Ken Nakajima, Masahiro Tatsumi, Tomohiko Iwasaki, Shinya Kosaka, Ida Shunichi, Hiroyuki Oigawa, Yoshihiro Yamane, Shigeaki Okajima, Yoichiro Shimazu, Shigeaki Aoki, Hideaki Ikeda, Akiko Yamamoto, Hiromi Maruyama, and Etsuro Saji
- Subjects
Physics ,Nuclear Energy and Engineering ,Nuclear engineering - Published
- 2003
15. Development and Verification of an Efficient Spatial Neutron Kinetics Method for Reactivity-Initiated Event Analyses
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Hideaki Ikeda and Toshikazu Takeda
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Predictor–corrector method ,Nuclear and High Energy Physics ,Computer science ,Astrophysics::High Energy Astrophysical Phenomena ,Computation ,Hardware_PERFORMANCEANDRELIABILITY ,Nuclear reactor ,law.invention ,Theoretical physics ,Nuclear Energy and Engineering ,Nuclear reactor core ,law ,Neutron flux ,Neutron ,Transient (oscillation) ,Diffusion (business) ,Algorithm - Abstract
A space/time nodal diffusion code based on the nodal expansion method (NEM), EPISODE, was developed in order to evaluate transient neutron behavior in light water reactor cores. The present code employs the improved quasi-static (IQS) method for spatial neutron kinetics, and neutron flux distribution is numerically obtained by solving the neutron diffusion equation with the nonlinear iteration scheme to achieve fast computation. A predictor-corrector (PC) method developed in the present study enabled to apply a coarse time mesh to the transient spatial neutron calculation than that applicable in the conventional IQS model, which improved computational efficiency further. Its computational advantage was demonstrated by applying to the numerical benchmark problems that simulate reactivity-initiated events, showing reduction of computational times up to a factor of three than the conventional IQS. The thermohydraulics model was also incorporated in EPISODE, and the capability of realistic reactivity event an...
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- 2001
16. Calculation of Doppler Reactivity Worth in Transient with Space Dependence of Self-shielding in Fuel Pellet
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Tadafumi Sano, Hideaki Ikeda, and Toshikazu Takeda
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symbols.namesake ,Nuclear Energy and Engineering ,Chemistry ,Pellet ,Analytical chemistry ,symbols ,Reactivity (chemistry) ,Transient (oscillation) ,Space (mathematics) ,Doppler effect ,Self shielding ,Computational physics - Published
- 1999
17. LIFE-CYCLE ASSESSMENT OF URBAN SEWERAGE SYSTEMS: A CASE STUDY USING 'LCE' AS AN INDICATOR
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Hidefumi Imura, Tetsuya Kusuda, Kanetoshi Morishita, Hideaki Ikeda, and Kenji Zenitani
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Engineering ,Work (electrical) ,business.industry ,Input–output model ,Sewerage ,Environmental engineering ,Sewage ,Environmental impact assessment ,Sanitary sewer ,Drainage ,business ,Life-cycle assessment ,Civil engineering - Abstract
The purpose of this study is to investigate the life-cycle environmental assessment of urban sewage systems. LCE (life-cycle energy) is chosen as an indicator, and energy input to construction and maintenance of water treatment plants and drainage networks is estimated. Energy required for the production of input materials, machines and equipments, and for the construction of buildings is calculated based on a standard method of input-output analysis. Direct energy input to construction works and plant operation is estimated by using the standard construction work budget estimation manual of the Ministry of Construction and the actual plant operation data for the sewers in Fukuoka City. Although the calculation does not cover the entire life-cycle and the functions of sewage systems, it reveals that about 83% of the estimated total LCE is required for the operation of treatment plants and the remaining 17% for the construction of treatment plants and drains.
- Published
- 1995
18. Comparative Study on the Environment Impacts Pertinent to Cnfrastructure Provision in Japan, China and South Korea
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Hidefumi Imura and Hideaki Ikeda
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Input–output model ,Natural resource economics ,business.industry ,Environmental protection ,Global warming ,Production (economics) ,Distribution (economics) ,East Asia ,Energy consumption ,business ,China ,Unit (housing) - Abstract
The energy consumption and the associated carbon dioxide emission induced by construction are calculated and compared among three east Asian countries including Japan, China and South Korea, where rapid economic development is taking place. Economic development is accompanied by improvement of various social infrastructures such as buildings and transportation systems. This induces the increasing energy demand for the production and distribution of materials such as iron and cement which are used for construction, and thus causes increasing impacts on the environment. Based on the standard input-output model, the energy consumption induced by unit final demand of construction sectors are calculated for the base year 1990. The results reveal that the construction sector as a whole plays an important role for energy consumption in each of the three countries. The share of the energy demand induced by construction sector in the total national energy consumption is 18% in Japan, while those in China and South Korea are 31% and 16% respectively.
- Published
- 1994
19. Measurement and correlation of mutual diffusion coefficients for styrene-butadiene rubber(SBR)-n-nonane systems
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Yoshio Iwai, Shinji Miyamoto, Hideaki Ikeda, Yasuhiko Arai, Shigetoshi Kobuchi, and Yuji Sano
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Polymers and Plastics ,Materials Chemistry ,General Chemistry - Published
- 1993
20. Study on the Quantification of Life Cycle Environmental Impacts Pertinent to Infrastructure Provision
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Hidefumi Imura and Hideaki Ikeda
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Estimation ,Energy conservation ,Input–output model ,business.industry ,Global warming ,Environmental resource management ,Economics ,Production (economics) ,Energy consumption ,Environmental economics ,business ,Embodied energy ,Unit (housing) - Abstract
The global warming has become a grave concern for mankind. Necessity of energy conservation in our daily life is advocated and policies for saving energy have been discussed in relation to urban environmental management. Here, growing attention has been paid to our lifestyles. In addition, however, we must consider the large amount of energy that are consumed for the improvement of urban infrastructures. Most of it is used through the input of materials such as irons and cements for construction works. From this viewpoint, the present study aims to analyze the energy consumption pertinent to urban infrastructure provision. Firstly, a method for analyzing such energy consumption is developed on the basis of the input-output model. Input of energy for a unit production expressed in monetary terms is calculated by using national input-output tables available today. It is shown that the input energy has been decreased remarkably during the 1955-1989 period in Japan. Secondly, estimation is made on the total energy value of the existing stocks of various infrastructures in the country. Thus this study aims to conduct the quantification of the life cycle environmental impacts related to the provision of various urban infrastructures.
- Published
- 1993
21. Solubilities of nonane vapor in styrene-butadiene copolymers at 100 and 130° C
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Hideaki Ikeda, Shinji Miyamoto, Yoshio Iwai, Yasuhiko Arai, and Toru Ishidao
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chemistry.chemical_classification ,Alkane ,Styrene-butadiene ,General Chemical Engineering ,General Physics and Astronomy ,Sorption ,chemistry.chemical_compound ,Hydrocarbon ,chemistry ,Copolymer ,Organic chemistry ,Physical and Theoretical Chemistry ,Nonane ,Solubility ,Aliphatic compound - Abstract
Iwai, Y., Ishidao, T., Miyamoto, S., Ikeda, H. and Arai, Y., 1991. Solubilities of nonane vapor in styrene-butadiene copolymers at 100 and 130 °C. Fluid Phase Equilibria 68: 197-205. The solubilities of nonane vapor in styrene-butadiene copolymers were measured with a sorption apparatus at 100 and 130 ° C for mass fractions of nonane ranging from 0 to 0.17. It is shown that the UNIFAC-FV model can be applied to predict the solubilities of nonane in styrene-butadiene copolymers.
- Published
- 1991
22. 3-D Neutron Transport Benchmarks
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Toshikazu TAKEDA and Hideaki IKEDA
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Nuclear and High Energy Physics ,Nuclear Energy and Engineering - Published
- 1991
23. BWR Stability Issues in Japan
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Shinya Mizokami, Hideaki Ikeda, Yuichiro Kubo, Hideo Soneda, and Takanori Fukahori
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Engineering ,Nuclear Energy and Engineering ,business.industry ,Nuclear engineering ,Code (cryptography) ,Stability (learning theory) ,Boiling water reactor ,Mathematics::Metric Geometry ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,business ,lcsh:TK1-9971 ,Physics::Geophysics - Abstract
The present paper reviews activates relevant to the boiling water reactor (BWR) stability phenomenon, which has a coupled neutronic and thermal-hydraulic nature, from the viewpoint of model and code developments and their applications to the BWR stability solution methodology in Japan.
- Published
- 2008
24. Lentivirus-based gene delivery in mouse embryonic stem cells
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Tadayoshi Ueda, Yuzuru Kurabayashi, Hideaki Ikeda, Noriaki Tanaka, Chen Yong, Kazuya Kobayashi, Masanobu Maruyama, Toshinori Totsugawa, Takashi Arata, Takuya Fukazawa, Naoya Kobayashi, and Yoshikazu Kosaka
- Subjects
Genetic Vectors ,Green Fluorescent Proteins ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Biology ,Gene delivery ,Green fluorescent protein ,Biomaterials ,Cell therapy ,Mice ,Multiplicity of infection ,Transduction, Genetic ,FuGENE ,medicine ,Animals ,Cells, Cultured ,Severe combined immunodeficiency ,Stem Cells ,Lentivirus ,Teratoma ,General Medicine ,medicine.disease ,Embryo, Mammalian ,Embryonic stem cell ,Molecular biology ,Luminescent Proteins ,Cell Transformation, Neoplastic ,Treatment Outcome ,Lac Operon ,Stem cell - Abstract
Background: Embryonic stem (ES) cells are widely used in therapeutic research as an unlimited source of cell therapy. Therefore, it is of great value to find a way to efficiently manipulate ES cells. HIV-1-derived lentiviral vectors are now considered to be an efficient vehicle for delivering genes into a variety of cells. In this study, we examined the efficacy of lentivirus-based gene delivery into mouse ES (mES) cells. Materials and methods: Recombinant HIV-I-based lentiviral vectors Lt-GFP, expressing green fluorescent protein (GFP), and Lt-LacZ, expressing E. coli LacZ gene in conjunction with neomycin resistance gene, were generated using a FuGENE 6 transduction method and used for transducing ES cells derived from 129Sv mice. Lentiviral transduction efficacy was evaluated by GFP expression assay using flow cytometry and by X-gal staining. The in vivo potential of developing teratoma of such transduced mES cells was examined in severe combined immunodeficiency (SCID) mice. Results: FuGENE 6 showed no considerable transduction-associated cytotoxicity. The expression rate of GFP and LacZ of mES cells increased on a multiplicity of infection (MOI)-dependent manner with the amount of Lt-GFP and Lt-LacZ used. Approximately 42% of mES cells were positive for GFP after infection of Lt-GFP at an MOI of 30. Notably, after G418 selection, nearly 100% of Lt-LacZ-transduced mES cells were positive for LacZ and formed teratomas in SCID mice. Conclusions: This work demonstrates that HIV-I-based lentiviral vectors are capable of transducing mES cells. Lentiviral vectors may facilitate an advance in the field of gene transfer and expression in various types of ES cells, including human ES cells.
- Published
- 2004
25. A Newly Developed Bioartificial Pancreas Successfully Controls Blood Glucose in Totally Pancreatectomized Diabetic Pigs
- Author
-
Teru Okitsu, Chen Yong, Hideaki Ikeda, Noriaki Tanaka, Michiki Narushima, Alejandro Soto-Gutierrez, Shuhei Nakaji, Koichi Tanaka, Yoshihito Tanaka, Nalu Navarro-Alvarez, Ji-Won Yoon, Jorge David Rivas-Carrillo, Shinichi Matsumoto, Hee-Sook Jun, Atsushi Miki, Kimiaki Tanaka, Hirofumi Noguchi, Mizuko Oshita, and Naoya Kobayashi
- Subjects
Blood Glucose ,Pancreas, Artificial ,medicine.medical_specialty ,animal structures ,Swine ,medicine.medical_treatment ,Diabetes Mellitus, Experimental ,Islets of Langerhans ,Mice ,In vivo ,Diabetes mellitus ,Internal medicine ,Insulin Secretion ,medicine ,Animals ,Insulin ,Cell adhesion ,geography ,geography.geographical_feature_category ,Bioartificial pancreas ,Bioartificial Organs ,Chemistry ,General Engineering ,Membranes, Artificial ,Islet ,medicine.disease ,In vitro ,medicine.anatomical_structure ,Endocrinology ,Pancreas - Abstract
Construction of a safe and functional bioartificial pancreas (BAP) that provides an adequate environment for islet cells may be an important approach to treating diabetic patients. Various types of BAP devices have been developed, but most of them involve extravascular implantation of islets in microcapsules or diffusion chambers. These devices have poor diffusive exchange between the islets and blood, and often rupture. To overcome these problems, we developed a new type of BAP composed of polyethylene-vinyl alcohol (EVAL) hollow fibers that are permeable to glucose and insulin and a poly-amino-urethane-coated, non-woven polytetrafluoroethylene (PTFE) fabric that allows cell adhesion. Porcine islets attached to the surface of the PTFE fabric, but not to the surface of the EVAL hollow fibers, allowing nutrient and oxygen exchange between blood flowing inside the fibers and cells outside. We inoculated this BAP with porcine islets and connected it to the circulation of totally pancreatectomized diabetic pigs. We found that blood glucose levels were reduced to a normal range and general health was improved, resulting in longer survival times. In addition, regulation of insulin secretion from the BAP was properly controlled in response to glucose both in vitro and in vivo. These results indicate that our newly developed BAP may be a potential therapy for the treatment of diabetes in humans.
- Published
- 2006
26. A case of atypical psychosis characterized by periodic stupor, with marked epileptic factors
- Author
-
Shigeaki Nishimura, Hideaki Ikeda, and Kiyoshi Hosokawa
- Subjects
Pediatrics ,medicine.medical_specialty ,ATYPICAL PSYCHOSIS ,business.industry ,Stupor ,medicine ,medicine.symptom ,business - Published
- 1969
27. Hiroshi Fukushima, 'People's Commune'
- Author
-
Hideaki, Ikeda
- Published
- 1968
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