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2. SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesothelioma

Author

Salaroglio, Iris Chiara, Belisario, Dimas Carolina, Bironzo, Paolo, Ananthanarayanan, Preeta, Ricci, Luisa, Digiovanni, Sabrina, Fontana, Simona, Napoli, Francesca, Sandri, Alberto, Facolmatà, Chiara, Libener, Roberta, Comunanza, Valentina, Grosso, Federica, Gazzano, Elena, Leo, Francesco, Taulli, Riccardo, Bussolino, Federico, Righi, Luisella, Papotti, Mauro Giulio, Novello, Silvia, Scagliotti, Giorgio Vittorio, Riganti, Chiara, and Kopecka, Joanna

Published
2022
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3. Changes of physico-chemical properties of nano-biomaterials by digestion fluids affect the physiological properties of epithelial intestinal cells and barrier models

Author

Antonello, Giulia, Marucco, Arianna, Gazzano, Elena, Kainourgios, Panagiotis, Ravagli, Costanza, Gonzalez-Paredes, Ana, Sprio, Simone, Padín-González, Esperanza, Soliman, Mahmoud G., Beal, David, Barbero, Francesco, Gasco, Paolo, Baldi, Giovanni, Carriere, Marie, Monopoli, Marco P., Charitidis, Costas A., Bergamaschi, Enrico, Fenoglio, Ivana, and Riganti, Chiara

Published
2022
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6. "Nitric Oxide Donors for Biomedical Applications: A Themed Issue Dedicated to Professor Alberto Gasco": Special Issue Editorial Overview.

Author

Sodano, Federica, Gazzano, Elena, and Fruttero, Roberta

Subjects
*NITRIC oxide, *COLLEGE teachers, *BACTERIAL diseases, *SMALL molecules, *PRODRUGS
Abstract

This document is a special issue editorial overview of the journal "Molecules" titled "Nitric Oxide Donors for Biomedical Applications: A Themed Issue Dedicated to Professor Alberto Gasco." The special issue focuses on the use of nitric oxide (NO) donors in biomedical applications. NO is a small molecule that plays a role in various physiological and pathophysiological processes in the body. The development of NO donors has shown therapeutic potential in cardiovascular pathologies, tumors, bacterial and microbial infections, and maintaining homeostasis in the gastrointestinal and respiratory tracts. The special issue includes contributions that cover a range of topics related to NO research, including the synthesis of NO donor prodrugs, the role of NO in various diseases, and reviews of NO-based therapies. The contributions offer interesting perspectives and highlight the roles of NO in health and disease. The special issue is dedicated to Professor Alberto Gasco for his contributions to NO prodrug research. [Extracted from the article]

Published
2023
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7. Corrigendum to ‘Glabratephrin reverses doxorubicin resistance in triple negative breast cancer by inhibiting P-glycoprotein’ [Pharmacol. Res. 175 (2022) 105975]

Author

Abd-ellatef, Gamal Eldein Fathy, primary, Gazzano, Elena, additional, El-Desoky, Ahmed H., additional, Hamed, Ahmed R., additional, Kopecka, Joanna, additional, Belisario, Dimas Carolina, additional, Costamagna, Costanzo, additional, Marie, Mohamed Assem S., additional, Fahmy, Sohair R., additional, Abdel-Hamid, Abdel-Hamid Z., additional, and Riganti, Chiara, additional

Published
2023
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9. Cytotoxicity of fibrous antigorite from New Caledonia

Author

Gazzano, Elena, primary, Petriglieri, Jasmine Rita, additional, Aldieri, Elisabetta, additional, Fubini, Bice, additional, Laporte-Magoni, Christine, additional, Pavan, Cristina, additional, Tomatis, Maura, additional, and Turci, Francesco, additional

Published
2022
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10. Efficacy, biocompatibility and degradability of carbon nanoparticles for photothermal therapy of lung cancer

Author

Kokalari, Ida, Keshavan, Sandeep, Rahman, Mizanur, Gazzano, Elena, Barzan, Giulia, Mandrile, Luisa, Giovannozzi, Andrea, Ponti, Jessica, Antonello, Giulia, Monopoli, Marco, Perrone, Guido, Bergamaschi, Enrico, Riganti, Chiara, Fadeel, Bengt, and Fenoglio, Ivana

Subjects
Programmed cell death, Lung Neoplasms, photothermal therapy, Biocompatibility, DNA damage, biodegradation, carbon-based nanomaterials, immune cells, lung cancer, Biomedical Engineering, Medicine (miscellaneous), Bioengineering, 02 engineering and technology, Development, 03 medical and health sciences, Immune system, Cell Line, Tumor, Heat shock protein, medicine, Humans, General Materials Science, Lung cancer, 030304 developmental biology, 0303 health sciences, Chemistry, Photothermal therapy, 021001 nanoscience & nanotechnology, medicine.disease, Carbon, 3. Good health, A549 Cells, Cancer research, Nanoparticles, 0210 nano-technology, Phototoxicity
Abstract

Aim: To investigate near infrared-induced phototoxicity toward lung cancer cells, and the biodegradability and effect on immune cells of glucose-derived carbon nanoparticles (CNPs). Methods: The human A549 lung adenocarcinoma cell line was used as a model to study the phototoxicity of CNPs. The biodegradability and the effect on immune cells was demonstrated in primary human neutrophils and macrophages. Results: Near infrared-activated CNPs elicited rapid cell death, characterized by the elevation of heat shock proteins and the induction of DNA damage. CNPs were found to be noncytotoxic toward primary human macrophages and were susceptible to biodegradation when cocultured with human neutrophils. Conclusions: Our results identify CNPs as promising platforms for photothermal therapy of lung cancer.

Published
2021
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12. Additional file 1 of Changes of physico-chemical properties of nano-biomaterials by digestion fluids affect the physiological properties of epithelial intestinal cells and barrier models

Author

Antonello, Giulia, Marucco, Arianna, Gazzano, Elena, Kainourgios, Panagiotis, Ravagli, Costanza, Gonzalez-Paredes, Ana, Sprio, Simone, Padín-González, Esperanza, Soliman, Mahmoud G., Beal, David, Barbero, Francesco, Gasco, Paolo, Baldi, Giovanni, Carriere, Marie, Monopoli, Marco P., Charitidis, Costas A., Bergamaschi, Enrico, Fenoglio, Ivana, and Riganti, Chiara

Abstract

Additional file 1. Figure S1. Optical appearance of the colloidal suspensions before A) and after B) treatment with the SHDS. Figure S2. Size distribution of the samples directly incubated in SIF or after complete SHDS-treatment of A) LSNPs; B) CNPs, C) FNPs; D) HNPs. Figure S3. HNPs dissolution. Figure S4. Degradation of LSNPs by lipase. Table S1. Mean dH and PDI of NMs in DMEM+10% FBS (100 mg/ml), 24 h incubation. Figure S5. Aspect of the suspension of the (*) untreated and (**) SHDS-treated A) LSNPs, B) CNPs, C) FNPs and D) HNPs in cell medium. Figure S6. SDS-PAGE analysis showing the hard corona of (*) SHDS-treated and (**) untreated A) LSNPs, B) CNPs, C) FNPs and D) HNPs in cell medium and obtained by centrifugation, and E) ctrl without NBMs. Figure S7. LFQ intensity for different proteases inhibitor for FNPs untreated and SHDS-treated. Figure S8. Cells viability of A) Caco-2 cells and B) HCoEpiC cells after 24 h of incubation with SHDS fluids. Figure S9. Genotoxicity of HCT116 cells after 24 h of incubation with untreated and SHDS-treated NMs. Table S2. Tran-Epithelial Electrical Resistance (TEER) values of Caco-2 barrier model after 24 h of incubation. Figure S10. Scheme of representative SDHS treatment.

Published
2022
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13. Additional file 1 of SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesothelioma

Author

Salaroglio, Iris Chiara, Belisario, Dimas Carolina, Bironzo, Paolo, Ananthanarayanan, Preeta, Ricci, Luisa, Digiovanni, Sabrina, Fontana, Simona, Napoli, Francesca, Sandri, Alberto, Facolmat��, Chiara, Libener, Roberta, Comunanza, Valentina, Grosso, Federica, Gazzano, Elena, Leo, Francesco, Taulli, Riccardo, Bussolino, Federico, Righi, Luisella, Papotti, Mauro Giulio, Novello, Silvia, Scagliotti, Giorgio Vittorio, Riganti, Chiara, and Kopecka, Joanna

Subjects
Data_FILES
Abstract

Additional file 1.

Published
2022
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14. Ketogal Safety Profile in Human Primary Colonic Epithelial Cells and in Mice

Author

Sodano, Federica, primary, Avallone, Bice, additional, Tizzano, Monica, additional, Fogliano, Chiara, additional, Rolando, Barbara, additional, Gazzano, Elena, additional, Riganti, Chiara, additional, Magliocca, Salvatore, additional, Cuozzo, Mariarosaria, additional, Albrizio, Stefania, additional, Calignano, Antonio, additional, Cristiano, Claudia, additional, Russo, Roberto, additional, and Rimoli, Maria Grazia, additional

Published
2021
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15. The heme synthesis-export system regulates the tricarboxylic acid cycle flux and oxidative phosphorylation

Author

Fiorito, Veronica, primary, Allocco, Anna Lucia, additional, Petrillo, Sara, additional, Gazzano, Elena, additional, Torretta, Simone, additional, Marchi, Saverio, additional, Destefanis, Francesca, additional, Pacelli, Consiglia, additional, Audrito, Valentina, additional, Provero, Paolo, additional, Medico, Enzo, additional, Chiabrando, Deborah, additional, Porporato, Paolo Ettore, additional, Cancelliere, Carlotta, additional, Bardelli, Alberto, additional, Trusolino, Livio, additional, Capitanio, Nazzareno, additional, Deaglio, Silvia, additional, Altruda, Fiorella, additional, Pinton, Paolo, additional, Cardaci, Simone, additional, Riganti, Chiara, additional, and Tolosano, Emanuela, additional

Published
2021
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17. Improvement of conventional anti-cancer drugs as new tools against multidrug resistant tumors

Author

Dallavalle, Sabrina, Dallavalle, Sabrina, Dobričić, Vladimir, Lazzarato, Loretta, Gazzano, Elena, Machuqueiro, Miguel, Pajeva, Ilza, Tsakovska, Ivanka, Zidar, Nace, Fruttero, Roberta, Dallavalle, Sabrina, Dallavalle, Sabrina, Dobričić, Vladimir, Lazzarato, Loretta, Gazzano, Elena, Machuqueiro, Miguel, Pajeva, Ilza, Tsakovska, Ivanka, Zidar, Nace, and Fruttero, Roberta

Abstract

Multidrug resistance (MDR) is the dominant cause of the failure of cancer chemotherapy. The design of antitumor drugs that are able to evade MDR is rapidly evolving, showing that this area of biomedical research attracts great interest in the scientific community. The current review explores promising recent approaches that have been developed with the aim of circumventing or overcoming MDR. Encouraging results have been obtained in the investigation of the MDR-modulating properties of various classes of natural compounds and their analogues. Inhibition of P-gp or downregulation of its expression have proven to be the main mechanisms by which MDR can be surmounted. The use of hybrid molecules that are able to simultaneously interact with two or more cancer cell targets is currently being explored as a means to circumvent drug resistance. This strategy is based on the design of hybrid compounds that are obtained either by merging the structural features of separate drugs, or by conjugating two drugs or pharmacophores via cleavable/non-cleavable linkers. The approach is highly promising due to the pharmacokinetic and pharmacodynamic advantages that can be achieved over the independent administration of the two individual components. However, it should be stressed that the task of obtaining successful multivalent drugs is a very challenging one. The conjugation of anticancer agents with nitric oxide (NO) donors has recently been developed, creating a particular class of hybrid that can combat tumor drug resistance. Appropriate NO donors have been shown to reverse drug resistance via nitration of ABC transporters and by interfering with a number of metabolic enzymes and signaling pathways. In fact, hybrid compounds that are produced by covalently attaching NO-donors and antitumor drugs have been shown to elicit a synergistic cytotoxic effect in a variety of drug resistant cancer cell lines. Another strategy to circumvent MDR is based on nanocarrier-mediated transport an

Published
2020

18. Identification and Preliminary Toxicological Assessment of a Non-regulated Mineral Fiber: Fibrous Antigorite from New Caledonia

Author

Petriglieri, J, Laporte-Magoni, C, Salvioli-Mariani, E, Tomatis, M, Gazzano, E, Turci, F, Cavallo, A, Fubini, B, Petriglieri, Jasmine Rita, Laporte-Magoni, Christine, Salvioli-Mariani, Emma, Tomatis, Maura, Gazzano, Elena, Turci, Francesco, Cavallo, Alessandro, Fubini, Bice, Petriglieri, J, Laporte-Magoni, C, Salvioli-Mariani, E, Tomatis, M, Gazzano, E, Turci, F, Cavallo, A, Fubini, B, Petriglieri, Jasmine Rita, Laporte-Magoni, Christine, Salvioli-Mariani, Emma, Tomatis, Maura, Gazzano, Elena, Turci, Francesco, Cavallo, Alessandro, and Fubini, Bice

Abstract

The rising awareness about the risk due to asbestos environmental exposure has led to a new interest in the investigation of non-regulated mineral fibers. Evidence of chronic diseases has been described in individuals exposed to naturally occurring asbestiform (NOA) minerals in Turkey (erionite), Italy (fluoro-edenite), and the United States (winchite/rictherite). In New Caledonia, an increased incidence of asbestos-related diseases was correlated with the natural occurrence of fibrous serpentines chrysotile and fibro-lamellar antigorite in outcrops, roadways, and soils. A minor amount of tremolite asbestos was also observed, increasing the health hazard. By adopting a precautionary principle, New Caledonia legislation classified antigorite as regulated asbestos, even if limited toxicity assessment is available. Caledonian antigorite exhibits a wide range of natural shapes, morphologies, and degrees of alteration as a result of pedogenic alteration induced by subtropical conditions. As the alteration increases, lamellar antigorite gradually cleaves into fibrous-like particles, assuming a fibro-lamellar habit. An increase in the emission of inhalable (potentially asbestiform) fibers in air was observed. To understand this mechanism, a multidisciplinary mineralogical and geochemical investigation was carried out. Additionally, several in vitro tests have been performed on three antigorite samples, subjected to different levels of alteration, to collect preliminary information on antigorite toxicity. Alteration modifies the surface reactivity of antigorite. The circulation of fluids induces a mechanical stress and an elemental exchange at mineral/water interface, promoting the loss of cohesion of the mineral structure and affecting the surface chemistry and toxicity of fibrous (asbestiform) antigorite.

Published
2020

21. Curcumin-Loaded Solid Lipid Nanoparticles Bypass P-Glycoprotein Mediated Doxorubicin Resistance in Triple Negative Breast Cancer Cells

Author

Fathy Abd-Ellatef, Gamal-Eldein, primary, Gazzano, Elena, additional, Chirio, Daniela, additional, Ragab Hamed, Ahmed, additional, Belisario, Dimas Carolina, additional, Zuddas, Carlo, additional, Peira, Elena, additional, Rolando, Barbara, additional, Kopecka, Joanna, additional, Assem Said Marie, Mohamed, additional, Sapino, Simona, additional, Ramadan Fahmy, Sohair, additional, Gallarate, Marina, additional, Zaki Abdel-Hamid, Abdel-Hamid, additional, and Riganti, Chiara, additional

Published
2020
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24. Additional file 4: of Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer

Author

Salaroglio, Iris, Gazzano, Elena, Abdullrahman, Ahmad, Mungo, Eleonora, Castella, Barbara, Abd-Elrahman, Gamal, Massaia, Massimo, Donadelli, Massimo, Rubinstein, Menachem, Riganti, Chiara, and Kopecka, Joanna

Abstract

Figure S3. Chloroquine and bortezomib increase nitrite levels. (DOCX 721â kb)

Published
2018
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25. Additional file 2: of Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer

Author

Salaroglio, Iris, Gazzano, Elena, Abdullrahman, Ahmad, Mungo, Eleonora, Castella, Barbara, Abd-Elrahman, Gamal, Massaia, Massimo, Donadelli, Massimo, Rubinstein, Menachem, Riganti, Chiara, and Kopecka, Joanna

Subjects
immune system diseases, hemic and lymphatic diseases
Abstract

Figure S1. Dose-dependence cell viability upon treatment with chloroquine and bortezomib. (DOCX 2931â kb)

Published
2018
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26. Additional file 1: of Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer

Author

Salaroglio, Iris, Gazzano, Elena, Abdullrahman, Ahmad, Mungo, Eleonora, Castella, Barbara, Abd-Elrahman, Gamal, Massaia, Massimo, Donadelli, Massimo, Rubinstein, Menachem, Riganti, Chiara, and Kopecka, Joanna

Subjects
polycyclic compounds
Abstract

Table S1 IC50 to doxorubicin in the cell lines analyzed. (DOCX 14â kb)

Published
2018
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27. Additional file 11: of Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer

Author

Salaroglio, Iris, Gazzano, Elena, Abdullrahman, Ahmad, Mungo, Eleonora, Castella, Barbara, Abd-Elrahman, Gamal, Massaia, Massimo, Donadelli, Massimo, Rubinstein, Menachem, Riganti, Chiara, and Kopecka, Joanna

Subjects
stomatognathic diseases, hemic and lymphatic diseases
Abstract

Table S2 Hematochemical parameters of animals treated with doxorubicin, chloroquine and bortezomib, in the presence of intratumorally induced C/EBP-β LIP. (DOCX 16 kb)

Published
2018
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28. Additional file 7: of Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer

Author

Salaroglio, Iris, Gazzano, Elena, Abdullrahman, Ahmad, Mungo, Eleonora, Castella, Barbara, Abd-Elrahman, Gamal, Massaia, Massimo, Donadelli, Massimo, Rubinstein, Menachem, Riganti, Chiara, and Kopecka, Joanna

Subjects
polycyclic compounds
Abstract

Figure S6. Set up and validation of an inducible C/EBP-β LIP expression system in Pgp-positive/doxorubicin-resistant JC cells. (DOCX 1224 kb)

Published
2018
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29. Additional file 9: of Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer

Author

Salaroglio, Iris, Gazzano, Elena, Abdullrahman, Ahmad, Mungo, Eleonora, Castella, Barbara, Abd-Elrahman, Gamal, Massaia, Massimo, Donadelli, Massimo, Rubinstein, Menachem, Riganti, Chiara, and Kopecka, Joanna

Subjects
integumentary system, polycyclic compounds
Abstract

Figure S8. Effects of CHOP silencing on nitric oxide production, Pgp expression and activity, calreticulin expression. (DOCX 3230â kb)

Published
2018
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30. Additional file 6: of Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer

Author

Salaroglio, Iris, Gazzano, Elena, Abdullrahman, Ahmad, Mungo, Eleonora, Castella, Barbara, Abd-Elrahman, Gamal, Massaia, Massimo, Donadelli, Massimo, Rubinstein, Menachem, Riganti, Chiara, and Kopecka, Joanna

Abstract

Figure S5. Chloroquine and bortezomib reversion of doxorubicin resistance in 3D-cultures. (DOCX 3366â kb)

Published
2018
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31. Additional file 5: of Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer

Author

Salaroglio, Iris, Gazzano, Elena, Abdullrahman, Ahmad, Mungo, Eleonora, Castella, Barbara, Abd-Elrahman, Gamal, Massaia, Massimo, Donadelli, Massimo, Rubinstein, Menachem, Riganti, Chiara, and Kopecka, Joanna

Subjects
immune system diseases, hemic and lymphatic diseases
Abstract

Figure S4. Effect of chloroquine, bortezomib, sodium nitroprusside and carboxy-PTIO on C/EBP-β LIP/CHOP/TRB3/caspase 3 axis in murine JC cells. (DOCX 799 kb)

Published
2018
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32. Additional file 3: of Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer

Author

Salaroglio, Iris, Gazzano, Elena, Abdullrahman, Ahmad, Mungo, Eleonora, Castella, Barbara, Abd-Elrahman, Gamal, Massaia, Massimo, Donadelli, Massimo, Rubinstein, Menachem, Riganti, Chiara, and Kopecka, Joanna

Subjects
immune system diseases, hemic and lymphatic diseases, cardiovascular diseases, neoplasms
Abstract

Figure S2. Chloroquine and bortezomib inhibition of lysosome and proteasome activity. (DOCX 1074â kb)

Published
2018
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33. Combination of PDT and NOPDT with a Tailored BODIPY Derivative

Author

Lazzarato, Loretta, primary, Gazzano, Elena, additional, Blangetti, Marco, additional, Fraix, Aurore, additional, Sodano, Federica, additional, Picone, Giulia Maria, additional, Fruttero, Roberta, additional, Gasco, Alberto, additional, Riganti, Chiara, additional, and Sortino, Salvatore, additional

Published
2019
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36. Correction to “Light-Regulated NO Release as a Novel Strategy To Overcome Doxorubicin Multidrug Resistance”

Author

Chegaev, Konstantin, primary, Fraix, Aurore, additional, Gazzano, Elena, additional, Abd-Ellatef, Gamal Eldein F., additional, Blangetti, Marco, additional, Rolando, Barbara, additional, Conoci, Sabrina, additional, Riganti, Chiara, additional, Fruttero, Roberta, additional, Gasco, Alberto, additional, and Sortino, Salvatore, additional

Published
2019
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38. iNOS activity is necessary for the cytotoxic and immunogenic effects of doxorubicin in human colon cancer cells

Author

Ghigo Dario, Matera Lina, Gazzano Elena, Brusa Davide, Kopecka Joanna, De Boo Sara, Bosia Amalia, and Riganti Chiara

Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background Doxorubicin is one of the few chemotherapeutic drugs able to exert both cytotoxic and pro-immunogenic effects against cancer cells. Following the drug administration, the intracellular protein calreticulin is translocated with an unknown mechanism onto the plasma membrane, where it triggers the phagocytosis of tumour cells by dendritic cells. Moreover doxorubicin up-regulates the inducible nitric oxide (NO) synthase (iNOS) gene in cancer cells, leading to huge amounts of NO, which in turn acts as a mediator of the drug toxicity and as a chemosensitizer agent in colon cancer. Indeed by nitrating tyrosine on the multidrug resistance related protein 3, NO decreases the doxorubicin efflux from tumour cells and enhances the drug toxicity. It is not clear if NO, beside playing a role in chemosensitivity, may also play a role in doxorubicin pro-immunogenic effects. To clarify this issue, we compared the doxorubicin-sensitive human colon cancer HT29 cells with the drug-resistant HT29-dx cells and the HT29 cells silenced for iNOS (HT29 iNOS-). Results In both HT29-dx and HT29 iNOS- cells, doxorubicin did not induce NO synthesis, had a lower intracellular accumulation and a lower toxicity. Moreover the drug failed to promote the translocation of calreticulin and the phagocytosis of HT29-dx and HT29 iNOS-cells, which resulted both chemoresistant and immunoresistant. However, if NO levels were exogenously increased by sodium nitroprusside, the chemosensitivity to doxorubicin was restored in HT29 iNOS-cells. In parallel the NO donor per se was sufficient to induce the exposure of calreticulin and to increase the phagocytosis of HT29 iNOS- cells by DCs and their functional maturation, thus mimicking the pro-immunogenic effects exerted by doxorubicin in the parental drug-sensitive HT29 cells. Conclusion Our data suggest that chemo- and immuno-resistance to anthracyclines are associated in colon cancer cells and rely on a common mechanism, that is the inability of doxorubicin to induce iNOS. Therefore NO donors might represent a promising strategy to restore both chemosensitivity and immunosensitivity to doxorubicin in resistant cells.

Published
2009
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39. Endoplasmic reticulum-targeting doxorubicin: a new tool effective against doxorubicin-resistant osteosarcoma

Author

Buondonno, Ilaria, primary, Gazzano, Elena, additional, Tavanti, Elisa, additional, Chegaev, Konstantin, additional, Kopecka, Joanna, additional, Fanelli, Marilù, additional, Rolando, Barbara, additional, Fruttero, Roberta, additional, Gasco, Alberto, additional, Hattinger, Claudia, additional, Serra, Massimo, additional, and Riganti, Chiara, additional

Published
2018
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44. Fibrinogen enhances the inflammatory response of alveolar macrophages to TiO2, SiO2 and carbon nanomaterials

Author

Marucco, Arianna, Gazzano, Elena, Ghigo, Dario, Enrico, Emanuele, and Fenoglio, Ivana

Abstract

Many studies have shown that the composition of the protein corona dramatically affects the response of cells to nanomaterials (NMs). However, the role of each single protein is still largely unknown. Fibrinogen (FG), one of the most abundant plasma proteins, is believed to mediate foreign-body reactions. Since this protein is absent in cell media used in in vitro toxicological tests the possible FG-mediated effects have not yet been assessed. Here, the effect of FG on the toxicity of three different kinds of inorganic NMs (carbon, SiO2 and TiO2) on alveolar macrophages has been investigated. A set of integrated techniques (UV–vis spectroscopy, dynamic light scattering and sodium dodecyl sulphate-polyacrylamide gel electrophoresis) have been used to study the strength and the kinetics of interaction of FG with the NMs. The inflammatory response of alveolar macrophages (MH-S) exposed to the three NMs associated with FG has also been investigated. We found that FG significantly enhances the cytotoxicity (lactate dehydrogenase leakage) and the inflammatory response (increase in nitric oxide (NO) concentration and NO synthase activation) induced by SiO2, carbon and TiO2 NMs on alveolar macrophages. This effect appears related to the amount of FG interacting with the NMs. In the case of carbon NMs, the activation of fibrinolysis, likely related to the exposure of cryptic sites of FG, was also observed after 24 h. These findings underline the critical role played by FG in the toxic response to NMs.

Published
2014
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45. Mitochondria-Targeted Doxorubicin: A New Therapeutic Strategy against Doxorubicin-Resistant Osteosarcoma

Author

Buondonno, Ilaria, primary, Gazzano, Elena, additional, Jean, Sae Rin, additional, Audrito, Valentina, additional, Kopecka, Joanna, additional, Fanelli, Marilù, additional, Salaroglio, Iris C., additional, Costamagna, Costanzo, additional, Roato, Ilaria, additional, Mungo, Eleonora, additional, Hattinger, Claudia M., additional, Deaglio, Silvia, additional, Kelley, Shana O., additional, Serra, Massimo, additional, and Riganti, Chiara, additional

Published
2016
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47. Zoledronic acid-encapsulating self-assembling nanoparticles and doxorubicin: a combinatorial approach to overcome simultaneously chemoresistance and immunoresistance in breast tumors

Author

Kopecka, Joanna, primary, Porto, Stefania, additional, Lusa, Sara, additional, Gazzano, Elena, additional, Salzano, Giuseppina, additional, Pinzòn-Daza, Martha Leonor, additional, Giordano, Antonio, additional, Desiderio, Vincenzo, additional, Ghigo, Dario, additional, De Rosa, Giuseppe, additional, Caraglia, Michele, additional, and Riganti, Chiara, additional

Published
2016
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48. Multi-walled carbon nanotubes directly induce epithelial-mesenchymal transition in human bronchial epithelial cells via the TGF-β-mediated Akt/GSK-3β/SNAIL-1 signalling pathway

Author

Polimeni, Manuela, primary, Gulino, Giulia Rossana, additional, Gazzano, Elena, additional, Kopecka, Joanna, additional, Marucco, Arianna, additional, Fenoglio, Ivana, additional, Cesano, Federico, additional, Campagnolo, Luisa, additional, Magrini, Andrea, additional, Pietroiusti, Antonio, additional, Ghigo, Dario, additional, and Aldieri, Elisabetta, additional

Published
2015
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49. Doxorubicin-antioxidant multitarget drugs

Author

Chegaev, Konstantin, Riganti, Chiara, Rolando, Barbara, Lazzarato, Loretta, Gazzano, Elena, Guglielmo, Stefano, Ghigo, Dario Antonio, Fruttero, Roberta, and Gasco, Alberto

Published
2013

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