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Wheat is one of the most important crops to ensure food production globally. Understanding the mechanism of leaf senescence in wheat plays a crucial role in improving its productivity and resilience under various stress scenarios. In this study, we investigated biochemical, functional, and ultrastructural changes during leaf senescence in wheat genotypes with contrasting drought tolerance. For this, key parameters such as chlorophyll and total protein content, membrane stability, malondialdehyde level, and the activity of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, benzidine peroxidase, and catalase) were comparatively analyzed during both natural and drought-induced senescence. Additionally, the expression of superoxide dismutase isoform genes functioning in different cellular compartments was studied, alongside ultrastructural changes in flag leaves. The experiments involved genotypes of bread wheat (Triticum aestivum L.) and durum (Triticum durum Desf.) wheat. The plants were grown in controlled environment chambers under control and drought conditions using a completely randomized design. After the booting stage, irrigation was discontinued for drought-treated plants. Flag leaves were sampled at 7, 14, 21, 28, and 35 days after anthesis. Drought-tolerant genotypes exhibited slower chlorophyll degradation, lower lipid peroxidation, enhanced membrane stability, and stronger antioxidant responses, allowing them to maintain cellular function longer, whereas sensitive genotypes showed accelerated leaf senescence. Transcript levels of FeSOD increased significantly post-flowering but declined as senescence progressed, while MnSOD expression exhibited a rise towards the later stages of ontogenesis across all studied genotypes. Ultrastructural analysis revealed progressive damage to chloroplast membranes, thylakoid structures, and mesophyll cell walls under stress conditions, particularly in sensitive genotypes. These findings contribute to a deeper understanding of the physiological and molecular responses of wheat to drought stress, offering potential targets for improving crop performance in water-limited environments. [ABSTRACT FROM AUTHOR]

In this study, pure silver nanowires (Ag NWs) were initially synthesized using the polyol method. Subsequently, Ag–Ag2S NWs were synthesized through hybridization at two different concentrations (0.01 and 0.001 M). The final product, Ag–Ag2S–CdS NWs with a one-dimensional (1D) unit structure, was obtained via an ion-exchange method using a Cd2+ cation source. To characterize these NWs, various techniques were employed: X-ray Diffraction (XRD), Ultraviolet–Visible (UV–Vis) spectroscopy, Fourier-transform infrared spectroscopy (FTIR), Photoluminescence (PL) spectroscopy, and Scanning Electron Microscopy (SEM) coupled with Energy Dispersive X-ray Spectroscopy (EDX) and, Transmission Electron Microscopy (TEM). The results confirmed the successful formation of the intended compounds through the synthesis process. TEM images verified the 1D core–shell structure of the NWs, with dimensions as follows: pure Ag NWs [D = 0.001 µm (1 nm)], Ag–Ag2S NWs (D = 0.52 µm), and Ag–Ag2S–CdS NWs (D = 0.61 µm). The PL spectrum revealed that pure Ag NWs exhibit high conductivity and surface plasmon resonance in the 400–500 nm range. The incorporation of semiconducting Ag2S altered the electron transition dynamics in the Ag–Ag2S system, attributed to surface defects in Ag2S. Additionally, in the presence of CdS, a decrease in intensity around 441 nm and spectrum broadening were observed, primarily due to Interband Damping. The degradation efficiency of Methylene Blue (MB) dye using Ag–Ag2S–CdS NWs as potential nanocatalysts was tested over various time intervals (60, 120, 180, 240, and 300 min) in three different environments (pH = 3, pH = 6, and pH = 10). The optimal degradation occurred in an acidic environment (pH = 3), achieving 97.9% efficiency at 300 min. The degradation kinetics were determined to be 0.0085 (60–120 min) and 0.0016 (120–300 min) in the acidic environment, 0.0029 in the neutral environment, and 0.0071 (60–180 min) and 0.001 (180–300 min) in the alkaline environment. [ABSTRACT FROM AUTHOR]

The widespread exposure of metallic nanoparticles to the aquatic ecosystem and its adverse impact on human life is the colossal concern worldwide. In view of this, this context was investigated to analyze microscopically the bioaccumulation and localization of magnetite (Fe 3 O 4 ) nanoparticles in the cellular organelles of rainbow trout ( Oncorhynchus mykiss , Walbaum, 1792) in aquatic conditions. Initially, Fe 3 O 4 nanoparticles were absorbed on to Elodea ( Elodea canadensis ) and fed to molluscs ( Melanopsis praemorsa ). Fish were fed with the same molluscs, and then the intestines and liver were examined using light and transmission electron microscopy. Results showed that nanoparticles were present in the cytoplasm and other organelles of cells (mitochondrion and lysosome) by absorbing through microvilli of the epithelial cells of the tunica mucosa in the intestine. Further, nanoparticles passed through the vessels of the lamina propria of the tunica mucosa and reached to the sinusoids of the liver via blood circulation. It was then accumulated from the endothelium of the sinusoid to the cytoplasm of liver hepatocytes and to mitochondria and lysosome. The accumulation of nanoparticles in the epithelial cells, cytoplasm, mitochondria, and lysosome revealed the degree of transparency of the pattern with slight hesitation. In summary, this investigation contributed towards the understanding of the physiological effects of Fe 3 O 4 nanoparticles on O. mykiss , which ascertains essentiality for sustainable development of nanobiotechnology in the aquatic ecosystem., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2020 The Author(s).)