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3. Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides

Author

Aitouguinane, Meriem, primary, El Alaoui-Talibi, Zainab, additional, Rchid, Halima, additional, Fendri, Imen, additional, Abdelkafi, Slim, additional, El-Hadj, Mohamed Didi Ould, additional, Boual, Zakaria, additional, Le Cerf, Didier, additional, Rihouey, Christophe, additional, Gardarin, Christine, additional, Dubessay, Pascal, additional, Michaud, Philippe, additional, Pierre, Guillaume, additional, Delattre, Cédric, additional, and El Modafar, Cherkaoui, additional

Published
2023
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4. Description of the Wild Strain Rhizobium rosettiformans DSM26376, Reclassified under Peteryoungia rosettiformans comb.nov., for Producing Glucuronan

Author

Christophe, Gwendoline, primary, Hou, Xiaoyang, additional, Petit, Emmanuel, additional, Traikia, Mounir, additional, Le Cerf, Didier, additional, Rihouey, Christophe, additional, Gardarin, Christine, additional, Delattre, Cédric, additional, Michaud, Philippe, additional, Pierre, Guillaume, additional, and Dubessay, Pascal, additional

Published
2023
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7. Exploring the Diversity of Red Microalgae for Exopolysaccharide Production

Author

Borjas Esqueda, Aldo Daniel, Gardarin, Christine, Laroche, Céline, Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), and Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA)

Subjects
Sulfates, Drug Discovery, Polysaccharides, Bacterial, Rhodophyta, Microalgae, exopolysaccharide, Pharmaceutical Science, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, microalgae, rhodophyta, Porphyridium, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), Culture Media
Abstract

International audience; Microalgae constitute a remarkable biological diversity but a limited number of them have been the object of study for their ability to produce exoplysaccharides (EPS). Among them, the red marine microalgae Porphyridium or Rhodella produce sulphated EPS, exhibiting some biological activities with potential interest in the pharmaceutical and cosmetic industries. EPS from Porphyridium and Rhodella being relatively similar in their composition, it has long been considered that all the red microalgae produced similar EPS and no attention was paid to other red microalgae. The objective of our work was then to explore the diversity of red microalgae for the production of EPS, focusing in this first step on the screening of the strains for their ability to produce EPS and preliminary structural characterization. The study was conducted with 11 microalgae strains belonging to the proteorhodophytina subphylum. All microalgae were able to produce EPS, released in the culture medium (strains belonging to Porphyridiophyceae and Rhodellophyceae classes) or remaining bound to the cells (strains from Stylonematophyceae class). The analysis of monosaccharides composition was found significantly different, with for instance high levels of glucuronic acids in the EPS from C. japonica and N. cyanea, but also strong differences in the sulphation degrees of polymers (between 1.2 and 28.7% eq. SO4).

Published
2022
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9. Biochemical Characterization of a Bifunctional Enzyme Constructed by the Fusion of a Glucuronan Lyase and a Chitinase from Trichoderma sp

Author

Baklouti, Zeineb, Delattre, Cédric, Pierre, Guillaume, Gardarin, Christine, Abdelkafi, Slim, Michaud, Philippe, Dubessay, Pascal, Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Département Génie Biologique, Université de Sfax, Unité de Biotechnologie des Algues, Ecole National d’Ingénieurs de Sfax, 3018 Sfax, Institut Universitaire de France (IUF), and Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)

Subjects
bifunctional enzyme, chitinase, polysaccharide, glucuronan lyase, lcsh:Q, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, enzymatic efficiency, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], lcsh:Science
Abstract

International audience; Bifunctional enzymes created by the fusion of a glucuronan lyase (TrGL) and a chitinase (ThCHIT42) from Trichoderma sp. have been constructed with the aim to validate a proof of concept regarding the potential of the chimera lyase/hydrolase by analyzing the functionality and the efficiency of the chimeric constructions compared to parental enzymes. All the chimeric enzymes, including or nor linker (GGGGS), were shown functional with activities equivalent or higher to native enzymes. The velocity of glucuronan lyase was considerably increased for chimeras, and may involved structural modifications at the active site. The fusion has induced a slightly decrease of the thermostability of glucuronan lyase, without modifying its catalytic activity regarding pH variations ranging from 5 to 8. The biochemical properties of chitinase seemed to be more disparate between the different fusion constructions suggesting an impact of the linkers or structural interactions with the linked glucuronan lyase. The chimeric enzymes displayed a decreased stability to temperature and pH variations, compared to parental one. Overall, TrGL-ThCHIT42 offered the better compromise in terms of biochemical stability and enhanced activity, and could be a promising candidate for further experiments in the field of fungi Cell Wall-Degrading Enzymes (CWDEs)

Published
2020
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10. Beneficial Health Potential of Algerian Polysaccharides Extracted from Plantago ciliata Desf. (Septentrional Sahara) Leaves and Seeds

Author

Addoun, Noura, primary, Boual, Zakaria, additional, Delattre, Cédric, additional, Chouana, Toufik, additional, Gardarin, Christine, additional, Dubessay, Pascal, additional, Benaoun, Fatima, additional, Addaoud, Seddik, additional, Ould El Hadj, Mohamed Didi, additional, Michaud, Philippe, additional, and Pierre, Guillaume, additional

Published
2021
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11. Optimization of Exopolysaccharides Production by Porphyridium sordidum and Their Potential to Induce Defense Responses in Arabidopsis thaliana against Fusarium oxysporum

Author

Drira, Marwa, primary, Elleuch, Jihen, additional, Ben Hlima, Hajer, additional, Hentati, Faiez, additional, Gardarin, Christine, additional, Rihouey, Christophe, additional, Le Cerf, Didier, additional, Michaud, Philippe, additional, Abdelkafi, Slim, additional, and Fendri, Imen, additional

Published
2021
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12. Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer

Author

Gargouch, Nesrine, primary, Elleuch, Fatma, additional, Karkouch, Ines, additional, Tabbene, Olfa, additional, Pichon, Chantal, additional, Gardarin, Christine, additional, Rihouey, Christophe, additional, Picton, Luc, additional, Abdelkafi, Slim, additional, Fendri, Imen, additional, and Laroche, Céline, additional

Published
2021
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13. Induction of Natural Defenses in Tomato Seedlings by Using Alginate and Oligoalginates Derivatives Extracted from Moroccan Brown Algae

Author

Aitouguinane, Meriem, primary, Bouissil, Soukaina, additional, Mouhoub, Anouar, additional, Rchid, Halima, additional, Fendri, Imen, additional, Abdelkafi, Slim, additional, Ould El-Hadj, Mohamed Didi, additional, Boual, Zakaria, additional, Dubessay, Pascal, additional, Gardarin, Christine, additional, Michaud, Philippe, additional, El Alaoui-Talibi, Zainab, additional, El Modafar, Cherkaoui, additional, Pierre, Guillaume, additional, and Delattre, Cédric, additional

Published
2020
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14. Prebiotic Activity of Poly- and Oligosaccharides Obtained from Plantago major L. Leaves

Author

Lukova, Paolina, primary, Nikolova, Mariana, additional, Petit, Emmanuel, additional, Elboutachfaiti, Redouan, additional, Vasileva, Tonka, additional, Katsarov, Plamen, additional, Manev, Hristo, additional, Gardarin, Christine, additional, Pierre, Guillaume, additional, Michaud, Philippe, additional, Iliev, Ilia, additional, and Delattre, Cédric, additional

Published
2020
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16. Research on the origin and the side effects of chitosan stabilizing properties in wine

Author

Dols, Marguerite, Paulin, Margot, Miot-Sertier, Cecile, Claisse, Olivier, Ballestra, Patricia, Albertin, Warren, Marchal, Axel, Masneuf, Isabelle, Delattre, Cédric, Pierre, Guillaume, Dubessay, Pascal, Gardarin, Christine, Michaud, Philippe, Doco, Thierry, Coulon, Joana, Massot, Arnaud, Vallet-Courbin, Amélie, Maupeu, Julie, Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Institut Pascal - Clermont Auvergne (IP), Sigma CLERMONT (Sigma CLERMONT)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Bio-Laffort, Microflora, Institut des Sciences de la Vigne et du Vin, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), and Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Vegetal Biology, vin, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, chitosan, wine, chitosane, Biologie végétale, Sciences agricoles, Agricultural sciences
Abstract

Research on the origin and the side effects of chitosan stabilizing properties in wine. Applied Natural Products : From Discoveries To Innovations

Published
2018

18. Characterization of a new exopolysaccharide produced by Halorubrumsp. TBZ112 and evaluation of its anti-proliferative effect on gastric cancer cells

Author

Hamidi, Masoud, Mirzaei, Rasool, Delattre, Cédric, Khanaki, Korosh, Pierre, Guillaume, Gardarin, Christine, Petit, Emmanuel, Karimitabar, Fatemeh, and Faezi, Sobhan

Abstract

In the present study, we aimed to extract, purify, analyze monosaccharide composition of  exopolysaccharide (EPS) produced by Halorubrumsp. TBZ112 (KCTC 4203 and IBRC-M 10773) and also to evaluate its possible antiproliferative activity against human gastric cancer (MKN-45) cell line and its biocompatibility effect on normal cells using human dermal fibroblast (HDF) cell line. Average molecular weight and monosaccharide composition were determined by high-pressure size exclusion chromatography (HPSEC) with multi-angle laser light scattering (MALLS) and high-pressure anion exchange chromatography (HPAEC), respectively. Fourier transform infrared (FTIR) spectroscopy was used for the partial characterization of the EPS. The EPS effect on the cell proliferation and viability of MKN-45 and HDF cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue dye exclusion, respectively. Strain TBZ112 excreted 480 mg.l−1of the EPS under optimal growth conditions. The EPS had a molecular weight of 5.052 kDa and was a heteropolysaccharide containing ten moieties mainly composed of mannose (19.95%), glucosamine (15.55%), galacturonic acid (15.43%), arabinose (12.24%), and glucuronic acid (12.05%). No significant difference of the EPS treatments on the proliferation activity of MKN-45 and HDF cells were observed (P> 0.05). For the first time, the EPS from Halorubrumsp. TBZ112, an extremely halophilic archaeon related to Halorubrumgenus, was isolated and chemically characterized. The EPS from Halorubrumsp. TBZ112 possesses a relatively low molecular weight and might be applied as a biocompatible compound. More investigations are needed to determine other biological activities of the EPS along with further details of its chemical structure.

Published
2019
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