19 results on '"Gallo, Marina"'
Search Results
2. Neurofilament light chain (Nf-L) in cerebrospinal fluid and serum as a potential biomarker in the differential diagnosis of neurological diseases in cattle.
- Author
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Di Muro, Giorgia, Tessarolo, Carlotta, Cagnotti, Giulia, Favole, Alessandra, Ferrini, Sara, Ala, Ugo, Bellino, Claudio, Borriello, Giuliano, Gallo, Marina, Iamone, Giulia, Iulini, Barbara, Pezzolato, Marzia, Casalone, Cristina, Caramelli, Maria, Capucci, Lorenzo, Cavadini, Patrizia, Corona, Cristiano, and D'Angelo, Antonio
- Abstract
Neurofilament light chain (Nf-L) is a biomarker for axonal damage in human neurology but is understudied in cattle. With this study we wanted to determine Nf-L stability at two different storage temperatures and Nf-L levels in healthy cattle and the relationship with age, evaluate whether Nf-L holds diagnostic potential for neurological disorders, and whether an association exists between Nf-L in serum and in cerebrospinal fluid (CSF). To do this, we measured Nf-L levels in CSF and serum samples from 49 healthy and 75 sick cattle. Storage at −80 °C or −20 °C had no impact on Nf-L concentration. Physiological median Nf-L levels were 6.3 pg/mL (serum) and 414 pg/mL (CSF) in calves and 5.5 pg/mL (serum) and 828 pg/mL (CSF) in adult cattle. There was no association between Nf-L levels in CSF and calf age (r
2 0.07, p = 0.13), while a weak association was found for Nf-L in serum (r2 0.26, p = 0.01), and a significant association in adult cattle (CSF, r2 0.69, p = 0.0001; serum, r2 0.68, p = 0.0003). CSF Nf-L levels were higher in samples from animals with degenerative (median Nf-L 49971 pg/mL) and infectious central nervous system (CNS) disorders (median Nf-L, age < 2 months 8863 pg/mL; age 2–12 months 17474 pg/mL; age 1–6 years 3546 pg/mL), CNS anomalies and metabolic/toxic disorders. There was a significant association between CSF Nf-L and serum Nf-L in cattle with neurological disorders (r2 0.2, p = 0.009). Taken together, these findings suggest the potential of Nf-L as a diagnostic tool in cattle neurology. [ABSTRACT FROM AUTHOR]- Published
- 2025
- Full Text
- View/download PDF
3. RT-QuIC detection of pathological prion protein in subclinical goats following experimental oral transmission of L-type BSE
- Author
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Favole, Alessandra, Mazza, Maria, D’Angelo, Antonio, Lombardi, Guerino, Palmitessa, Claudia, Dell’Atti, Luana, Cagnotti, Giulia, Berrone, Elena, Gallo, Marina, Avanzato, Tiziana, Messana, Erika, Masoero, Loretta, Acutis, Pier Luigi, Meloni, Daniela, Cardone, Franco, Caramelli, Maria, Casalone, Cristina, and Corona, Cristiano
- Published
- 2021
- Full Text
- View/download PDF
4. Resultados clínicos y funcionales de la artroplastia total de rodilla Low Contact Stress (LCS®) con un mínimo de 5 años de seguimiento
- Author
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Carbó-Laso, Esther, Sanz-Ruiz, Pablo, Quevedo-Narciso, Tania, Quiroga-Montes, Luis, Benito-Gallo, Marina, and Vaquero-Martín, Javier
- Published
- 2016
- Full Text
- View/download PDF
5. Early and Pre-Clinical Detection of Prion Seeding Activity in Cerebrospinal Fluid of Goats using Real-Time Quaking-Induced Conversion Assay
- Author
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Favole, Alessandra, Mazza, Maria, Vallino Costassa, Elena, D’Angelo, Antonio, Lombardi, Guerino, Marconi, Paola, Crociara, Paola, Berrone, Elena, Gallo, Marina, Palmitessa, Claudia, Orrù, Christina D., Caughey, Byron, Acutis, Pier L., Caramelli, Maria, Casalone, Cristina, and Corona, Cristiano
- Published
- 2019
- Full Text
- View/download PDF
6. Brain-Derived Neurotrophic Factor, Kynurenine Pathway, and Lipid-Profiling Alterations as Potential Animal Welfare Indicators in Dairy Cattle
- Author
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Favole, Alessandra, primary, Testori, Camilla, additional, Bergagna, Stefania, additional, Gennero, Maria Silvia, additional, Ingravalle, Francesco, additional, Costa, Barbara, additional, Barresi, Sara, additional, Curti, Piercarlo, additional, Barberis, Francesco, additional, Ganio, Sandra, additional, Orusa, Riccardo, additional, Vallino Costassa, Elena, additional, Berrone, Elena, additional, Vernè, Marco, additional, Scaglia, Massimo, additional, Palmitessa, Claudia, additional, Gallo, Marina, additional, Tessarolo, Carlotta, additional, Pederiva, Sabina, additional, Ferrari, Alessio, additional, Lorenzi, Valentina, additional, Fusi, Francesca, additional, Brunelli, Laura, additional, Pastorelli, Roberta, additional, Cagnotti, Giulia, additional, Casalone, Cristina, additional, Caramelli, Maria, additional, and Corona, Cristiano, additional
- Published
- 2023
- Full Text
- View/download PDF
7. SOMAscan Proteomics Identifies Novel Plasma Proteins in Amyotrophic Lateral Sclerosis Patients
- Author
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Berrone, Elena, primary, Chiorino, Giovanna, additional, Guana, Francesca, additional, Benedetti, Valerio, additional, Palmitessa, Claudia, additional, Gallo, Marina, additional, Calvo, Andrea, additional, Casale, Federico, additional, Manera, Umberto, additional, Favole, Alessandra, additional, Crociara, Paola, additional, Testori, Camilla, additional, Carta, Valerio, additional, Tessarolo, Carlotta, additional, D’Angelo, Antonio, additional, De Marco, Giovanni, additional, Caramelli, Maria, additional, Chiò, Adriano, additional, Casalone, Cristina, additional, and Corona, Cristiano, additional
- Published
- 2023
- Full Text
- View/download PDF
8. Pro-Inflammatory and Cytotoxic Effects of Polystyrene Microplastics on Human and Murine Intestinal Cell Lines
- Author
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Mattioda, Virginia, primary, Benedetti, Valerio, additional, Tessarolo, Carlotta, additional, Oberto, Francesca, additional, Favole, Alessandra, additional, Gallo, Marina, additional, Martelli, Walter, additional, Crescio, Maria Ines, additional, Berio, Enrica, additional, Masoero, Loretta, additional, Benedetto, Alessandro, additional, Pezzolato, Marzia, additional, Bozzetta, Elena, additional, Grattarola, Carla, additional, Casalone, Cristina, additional, Corona, Cristiano, additional, and Giorda, Federica, additional
- Published
- 2023
- Full Text
- View/download PDF
9. Spinal fusion achieves similar two-year improvement in HRQoL as total hip and total knee replacement. A prospective, multicentric and observational study
- Author
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Cervera Irimia Javier, Tomé-Bermejo Félix, Piñera-Parrilla Angel R., Benito Gallo Marina, Bisaccia Michele, Fernández-González Manuel, Villar-Pérez Julio, Fernández-Carreira Jose Manuel, Orovio de Elizaga Javier, Areta-Jiménez Francisco Javier, Álvarez Galovich Luis, Rollo Giuseppe, Caruso Luigi, and Meccariello Luigi
- Subjects
Hip arthroplasty ,Knee arthroplasty ,Spinal surgery ,Quality of life ,Functional assessment ,Orthopedic surgery ,RD701-811 - Abstract
Introduction: Total hip and knee arthroplasty (THA/TKA) are surgical procedures with proven benefits. Although the literature reports outcomes of fusion of the lumbar spine comparable to those of THA/TKA in general health-related quality-of-life (HRQoL) questionnaires, functional assessment is nevertheless needed for these results to be of use in clinical practice and management. Aim of our study was to prove that lumbar spinal fusion has similar if not better outcomes than THA/TKA using intervention-specific HRQoL questionnaires and functional assessment questionnaires. Materials and methods: Observational, ambispective, multicentre study of three cohorts undergoing lumbar spinal fusion (n = 115), THA (n = 119) and TKA (n = 253). Patients were evaluated using the Short-Form-12 (SF-12), Harris–Hip-Score, Hospital for Special Surgery Scale (HSS) and Oswestry Low Back Pain Disability questionnaires. A minimum follow-up of two years was conducted. Results: The SF-12 showed significant improvement in all groups. The SF-12 physical component summary score indicated a more severe pre-operative status (p = 0.031) in the THA cohort. The mental component summary score indicated a less severe pre-operative status in the TKA cohort (p = 0.008) and greater post-operative improvement in the TKA and THA cohorts across follow-up (six months p = 0.021; one year p = 0.012; two years p = 0.042). Functional assessment indicated greater pre-operative disability in the THA group. At two years of follow-up, functional improvement according to the Harris, HSS and Oswestry questionnaires were 152.01%, 50.07% and 41.14% respectively. Conclusions: This study demonstrates that lumbar spinal fusion and total knee and hip arthroplasty are comparable in terms of functional improvement when thoroughly studied with health, quality-of-life and functional assessment questionnaires.
- Published
- 2019
- Full Text
- View/download PDF
10. Cool as ice: standout bags and bling--mixed, matched, and layered to the max--give your wardrobe a major wake-up call
- Author
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Gallo, Marina
- Subjects
Chanel S.A. ,Tiffany & Co. ,Clothing industry ,Specialty stores ,Fashion and beauty ,Women's issues/gender studies - Abstract
TINSEL TOWN A metallic-silver tote signals fearless glamour. DRESS, $3,350, CALVIN KLEIN COLLECTION; PANTS, $995, MICHAEL KORS COLLECTION; EARRINGS, PRICE UPON REQUEST, DIOR FINE JEWELRY; NECKLACE, PRICE UPON REQUEST, CHANEL [...]
- Published
- 2016
11. Evaluación del hábitat físico para la especie Lefua echigonia mediante simulación en el río Yagawa, Japón
- Author
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Miguel Gallo, Marina de
- Subjects
Ecohidráulica ,Urban River ,Máster Universitario en Ingeniería Hidráulica y Medio Ambiente-Màster Universitari en Enginyeria Hidràulica i Medi Ambient ,Biodiversity ,Urban river ,Simulación de Hábitat ,Especie en Peligro de Extinción ,Biodiversidad ,Sostenibilidad ,Endangered species ,Sustainability ,Río Urbano ,Ecohydraulics ,Lefua echigonia ,Habitat simulation ,TECNOLOGIA DEL MEDIO AMBIENTE - Abstract
El rápido desarrollo que ha tenido lugar en los últimos años, como consecuencia del incremento poblacional y la aparición de distintos avances tecnológicos, tiene como consecuencia directa una degradación de los ecosistemas naturales, y por tanto una pérdida de la biodiversidad. Las actividades humanas, tales como la urbanización o la abstracción de agua resultan en presiones ejercidas sobre los sistemas naturales, sobre los que cabe destacar los ecosistemas acuáticos. Estas presiones pueden presentarse en diversas formas, alterando el ecosistema y dificultando a las especies que habitan en él el completar sus ciclos vitales y perpetuarse en el tiempo, conllevando así una pérdida de la biodiversidad, y por tanto de la resiliencia del sistema. En este contexto, Tokio adquiere un gran importancia, por ser una de las ciudades con mayor extensión del mundomás de 2000km2), representando por tanto un problema de gran envergadura en Japón. Dada la enorme extensión que ocupa, afectando a una gran proporción del terreno japonés, y dado que la tendencia es creciente, es de vital importancia recuperar y mantener su biodiversidad, realizando actuaciones que ayuden a mejorar las condiciones de estos ecosistemas profundamente alterados. Por eso, este estudio se centra en un pequeño río urbano, en el que habita Lefua echigonia, una especie nativa de Japón en peligro de extinción, con el objetivo de simular su hábitat, y proponer alternativas de mejora que ayuden a proteger esta especie, y mantener la biodiversidad. Para ello, se ha elaborado un modelo de idoneidad de hábitat, basado en curvas de idoneidad para Lefua echigonia, y se ha evaluado el hábitat a lo largo de un periodo temporal de 24 meses. Este estudio aporta por tanto una información muy valiosa en aras de mantener y recuperar la biodiversidad en un entorno tan grande como el de Tokio, la urbe más grande del planeta; dado que es uno de los primeros estudios de idoneidad de microhábitat realizados en Japón, al margen de los salmónidos, muy estudiados en todo el mundo, centrándose en una especie endémica en peligro de extinción, de gran importancia para la sostenibilidad de los ecosistemas; y siendo al mismo tiempo fundamental en la realización de estudios de caudales ecológicos posteriores. Por otro lado, al haberse realizado en un río urbano, adquiere una gran importancia, al estudiar así un tipo de río muy presente en esta gran metrópolis, que a pesar de haber sido normalmente ignorados en este tipo de estudios, son de vital importancia en el mantenimiento de ecosistemas clave para ciertas especies que en ellos habitan. Al mismo tiempo, las medidas de restauración propuestas para el caso de estudio pueden ser extrapolables a otros ríos cercanos que presenten la misma problemática., The rapid development that has taken place during the last decades, as a result of the population increase and the appearance of different technological advances, is a direct cause of the natural ecosystem degradation. Human activities, such as urbanization or water abstraction, result in pressures exerted on natural systems, among which aquatic ecosystems can be highlighted. These pressures affect the ecosystems in different ways, altering them or making it difficult for the inhabiting species to complete their life cycles, and last in time; leading to a loss of biodiversity and therefore to the resilience of the system. In this context, Tokyo acquires a great importance, being one of the largest cities in the world (over 2000 km2), turning into a big problem in Japan. Due to the enormous area that it occupies, affecting a large proportion of the Japanese land, and given the growing trend, it is essential to recover and maintain its biodiversity, performing actions that help improving the habitat conditions of this deeply disturbed ecosystems. This study focuses on a small urban river, inhabited by Lefua echigonia, an endangered native species from Japan, in order to simulate its habitat, and proposing different alternatives for improvement, to protect this species and maintain biodiversity. To this aim, a habitat suitability model was developed, based on suitability curves, and the habitat was evaluated for a 24-months-period. Therefore, this study provides valuable information in order to maintain and recover biodiversity in an environment as large as the one of Tokyo, along with the fact that it is one of the first microhabitat suitability studies carried in Japan (apart from the salmonids ones, very studied around the world), focusing on an endemic endangered species with a great importance for the ecosystems sustainability. The information obtained is essential while realizing later ecological flows studies accomplishment. As it was carried in an urban river, it acquires a great importance when studying similar rivers, with a great presence in this metropolis, which, in spite of having been often ignored in this kind of studies, show a vital importance in the maintenance of the key ecosystems for certain species. At the same time, the proposed restoration measures for the case of study may be extrapolated to other nearby rivers presenting the same problem.
- Published
- 2018
12. Clinical, pathological, and molecular features of classical and L-type atypical-BSE in goats
- Author
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Vallino Costassa, Elena, primary, D’Angelo, Antonio, additional, Mazza, Maria, additional, Meloni, Daniela, additional, Baioni, Elisa, additional, Maurella, Cristiana, additional, Colussi, Silvia, additional, Martinelli, Nicola, additional, Lo Faro, Monica, additional, Berrone, Elena, additional, Favole, Alessandra, additional, Crociara, Paola, additional, Grifoni, Silvia, additional, Gallo, Marina, additional, Lombardi, Guerino, additional, Iulini, Barbara, additional, Casalone, Cristina, additional, and Corona, Cristiano, additional
- Published
- 2018
- Full Text
- View/download PDF
13. Evaluación del hábitat físico para la especie Lefua echigonia mediante simulación en el río Yagawa, Japón
- Author
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Martínez Capel, Francisco, Fukuda, Shinji, Kajisa, Takamitsu, Universitat Politècnica de València. Departamento de Ingeniería Hidráulica y Medio Ambiente - Departament d'Enginyeria Hidràulica i Medi Ambient, Miguel Gallo, Marina de, Martínez Capel, Francisco, Fukuda, Shinji, Kajisa, Takamitsu, Universitat Politècnica de València. Departamento de Ingeniería Hidráulica y Medio Ambiente - Departament d'Enginyeria Hidràulica i Medi Ambient, and Miguel Gallo, Marina de
- Abstract
El rápido desarrollo que ha tenido lugar en los últimos años, como consecuencia del incremento poblacional y la aparición de distintos avances tecnológicos, tiene como consecuencia directa una degradación de los ecosistemas naturales, y por tanto una pérdida de la biodiversidad. Las actividades humanas, tales como la urbanización o la abstracción de agua resultan en presiones ejercidas sobre los sistemas naturales, sobre los que cabe destacar los ecosistemas acuáticos. Estas presiones pueden presentarse en diversas formas, alterando el ecosistema y dificultando a las especies que habitan en él el completar sus ciclos vitales y perpetuarse en el tiempo, conllevando así una pérdida de la biodiversidad, y por tanto de la resiliencia del sistema. En este contexto, Tokio adquiere un gran importancia, por ser una de las ciudades con mayor extensión del mundomás de 2000km2), representando por tanto un problema de gran envergadura en Japón. Dada la enorme extensión que ocupa, afectando a una gran proporción del terreno japonés, y dado que la tendencia es creciente, es de vital importancia recuperar y mantener su biodiversidad, realizando actuaciones que ayuden a mejorar las condiciones de estos ecosistemas profundamente alterados. Por eso, este estudio se centra en un pequeño río urbano, en el que habita Lefua echigonia, una especie nativa de Japón en peligro de extinción, con el objetivo de simular su hábitat, y proponer alternativas de mejora que ayuden a proteger esta especie, y mantener la biodiversidad. Para ello, se ha elaborado un modelo de idoneidad de hábitat, basado en curvas de idoneidad para Lefua echigonia, y se ha evaluado el hábitat a lo largo de un periodo temporal de 24 meses. Este estudio aporta por tanto una información muy valiosa en aras de mantener y recuperar la biodiversidad en un entorno tan grande como el de Tokio, la urbe más grande del planeta; dado que es uno de los primeros estudios de idoneidad de microhábitat realizados en Japón, al margen de, The rapid development that has taken place during the last decades, as a result of the population increase and the appearance of different technological advances, is a direct cause of the natural ecosystem degradation. Human activities, such as urbanization or water abstraction, result in pressures exerted on natural systems, among which aquatic ecosystems can be highlighted. These pressures affect the ecosystems in different ways, altering them or making it difficult for the inhabiting species to complete their life cycles, and last in time; leading to a loss of biodiversity and therefore to the resilience of the system. In this context, Tokyo acquires a great importance, being one of the largest cities in the world (over 2000 km2), turning into a big problem in Japan. Due to the enormous area that it occupies, affecting a large proportion of the Japanese land, and given the growing trend, it is essential to recover and maintain its biodiversity, performing actions that help improving the habitat conditions of this deeply disturbed ecosystems. This study focuses on a small urban river, inhabited by Lefua echigonia, an endangered native species from Japan, in order to simulate its habitat, and proposing different alternatives for improvement, to protect this species and maintain biodiversity. To this aim, a habitat suitability model was developed, based on suitability curves, and the habitat was evaluated for a 24-months-period. Therefore, this study provides valuable information in order to maintain and recover biodiversity in an environment as large as the one of Tokyo, along with the fact that it is one of the first microhabitat suitability studies carried in Japan (apart from the salmonids ones, very studied around the world), focusing on an endemic endangered species with a great importance for the ecosystems sustainability. The information obtained is essential while realizing later ecological flows studies accomplishment. As it was carried in an urban river
- Published
- 2018
14. Francesca Woodman e o lugar de onde eu me olho
- Author
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GALLO, Marina Didier Nunes and COUTINHO, Marcelo Farias
- Subjects
Fotógrafos ,Arte ,Fotografia ,Woodman, Francesca - Crítica e interpretação ,Feminismo e Arte ,Identidade de gênero - Abstract
CAPES A partir da leitura das obras da fotógrafa americana Francesca Woodman, passo a tecer diálogos com a temática das categorizações sociais femininas, da identidade e da singularidade, com o intuito de refletir sobre o modo como a produção de subjetividade encontra, contemporaneamente, seus obstáculos, e como as instituições tendem a dificultar tal produção. Ao percorrer as imagens de Francesca, nas quais ela questiona e confronta padrões femininos estabelecidos dentro do seu contexto histórico, falo da representação fotográfica como meio de construção das identidades e da arte como um dos lugares no qual certas construções naturalizadas podem ser quebradas e confrontadas, e onde a singularidade pode ser expressada. Entre o ensaio, as fotografias e os trechos de diários, meu e dela, reflito sobre as influências desses ordenamentos sociais nas produções das imagens que as mulheres fazem de si mesmas. By taking as a guideline tread the works of the American photographer Francesca Woodman, I weave dialogues with issues such as women's social categorizations, identity and uniqueness, in order to reflect on how the production of subjectivity faces, nowadays, its obstacles, and how institutions tend to hinder such production. By scrolling through the pictures of Francesca, in which she questions and confronts female standards set within her historical context, I speak of photographic representation as a means to construction of identities and art as one of the places in which certain naturalized constructions can be broken and confronted, and where the uniqueness can be expressed. Among the essay, the photographs and journal entries, hers and mine, I raise considerations regarding the influences of these social standards and stereotyping upon the images produced by female artists.
- Published
- 2015
15. Recursos televisivos en la Educación Infantil. Propuesta de intervención
- Author
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Torinos Gallo, Marina, Rodríguez García, Luis Carlos, and Universidad de Valladolid. Facultad de Educación y Trabajo Social
- Subjects
[Pendiente de asignar] - Abstract
En el presente Proyecto se recogen una serie de reflexiones e iniciativas con el fin de favorecer la integración curricular de la programación infantil en los procesos de enseñanza aprendizaje que tienen lugar en esta etapa, empleándolo como material didáctico que mejore la práctica pedagógica. Se trata pues, de utilizar los recursos televisivos como motivación educativa, inculcando conocimientos e ideas a través del entorno de dispersión y esparcimiento del niño/a. Con este fin, expondremos inicialmente las opiniones de los autores más representativos en este terreno. Hablaremos además sobre la importancia de las Nuevas Tecnologías en la Educación, destacando el papel que debe ejercer el docente. Finalmente, señalaremos posibles actividades y experiencias para enriquecer el aprendizaje de los alumnos/as, dando a conocer las diversas aplicaciones de la TV en la enseñanza, y plantearemos una intervención educativa basada en la inclusión de los recursos televisivos en la práctica docente, centrándonos en una serie actual en particular., Grado en Educación Infantil
- Published
- 2014
16. Olfactory System Involvement in Natural Scrapie Disease
- Author
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Corona, Cristiano, primary, Porcario, Chiara, additional, Martucci, Francesca, additional, Iulini, Barbara, additional, Manea, Barbara, additional, Gallo, Marina, additional, Palmitessa, Claudia, additional, Maurella, Cristiana, additional, Mazza, Maria, additional, Pezzolato, Marzia, additional, Acutis, Pierluigi, additional, and Casalone, Cristina, additional
- Published
- 2009
- Full Text
- View/download PDF
17. P.123: Experimental transmission of classical and atypical BSE in goats.
- Author
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Costassa, Elena Vallino, D'Angelo, Antonio, Martinelli, Nicola, Lombardi, Guerino, Pintore, Maria Domenica, Iulini, Barbara, Gallo, Marina, Peletto, Simone, Acutis, Pier Luigi, Meloni, Daniela, Mazza, Maria, Casalone, Cristina, and Corona, Cristiano
- Published
- 2014
18. P.85: Transcriptome analysis by microarray and RT-qPCR of blood from BSE-infected cattle.
- Author
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Barbisin, Maura, Xerxa, Elena, Chieppa, Maria Novella, Krmac, Helena, Vatta, Paolo, Vallino Costassa, Elena, Gallo, Marina, Casalone, Cristina, Simmons, Marion M., Legname, Giuseppe, and Corona, Cristiano
- Published
- 2014
19. Characterization of amyloid-β deposits in bovine brains
- Author
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Daniela Meloni, Marcella Catania, Maria Novella Chieppa, Elena Vallino Costassa, Roberta Ghidoni, Orlando Paciello, Gianluigi Zanusso, Fabrizio Tagliavini, Pier Luigi Acutis, Cristiano Corona, Antonio D'Angelo, Elisa Tonoli, Cristina Casalone, Marina Gallo, Monica Lo Faro, Cristiana Maurella, Michele Fiorini, Elisa Baioni, Simone Peletto, Vallino Costassa, Elena, Fiorini, Michele, Zanusso, Gianluigi, Peletto, Simone, Acutis, Pierluigi, Baioni, Elisa, Maurella, Cristiana, Tagliavini, Fabrizio, Catania, Marcella, Gallo, Marina, Faro, Monica Lo, Chieppa, Maria Novella, Meloni, Daniela, D'Angelo, Antonio, Paciello, Orlando, Ghidoni, Roberta, Tonoli, Elisa, Casalone, Cristina, and Corona, Cristiano
- Subjects
0301 basic medicine ,Apolipoprotein E ,Pathology ,Amyloid beta-Peptide ,Genotyping Techniques ,Intracellular Space ,Hippocampus ,0302 clinical medicine ,Gene Frequency ,PSEN1 ,aging ,amyloid beta-protein ,cattle ,glial cells ,Neurons ,medicine.diagnostic_test ,General Neuroscience ,Brain ,General Medicine ,Human brain ,Immunohistochemistry ,Psychiatry and Mental health ,Clinical Psychology ,medicine.anatomical_structure ,Neuroglia ,Intracellular ,Research Article ,medicine.medical_specialty ,Blotting, Western ,Biology ,Presenilin ,03 medical and health sciences ,Apolipoproteins E ,Western blot ,Glial Fibrillary Acidic Protein ,Presenilin-2 ,Extracellular ,medicine ,Presenilin-1 ,Animals ,Amyloid beta-Peptides ,Polymorphism, Genetic ,Animal ,glial cell ,Neuron ,Molecular biology ,030104 developmental biology ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Geriatrics and Gerontology ,Genotyping Technique ,Extracellular Space ,030217 neurology & neurosurgery - Abstract
Amyloid-β (Aβ) deposits are seen in aged individuals of many mammalian species that possess the same aminoacid sequence as humans. This study describes Aβ deposition in 102 clinically characterized cattle brains from animals aged 0 to 20 years. Extracellular and intracellular Aβ deposition was detected with 4G8 antibody in the cortex, hippocampus, and cerebellum. X-34 staining failed to stain Aβ deposits, indicating the non β-pleated nature of these deposits. Western blot analysis and surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry revealed in Tris, Triton, and formic acid fractions the presence of different Aβ peptides, characterized mainly by C-terminally truncated forms. Exploration of the genetic variability of APOE, PSEN1, and PSEN2 genes involved in Alzheimer's disease pathogenesis revealed several previously unreported polymorphisms. This study demonstrates certain similarities between Aβ deposition patterns exhibited in cattle brains and those in the human brain in early stages of aging. Furthermore, the identification of the same Aβ peptides reported in humans, but unable to form aggregates, supports the hypothesis that cattle may be protected against amyloid plaque formation.
- Published
- 2016
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