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Angiogenesis is a key event in the progression of gliomas, and emerging evidence suggests that exosomes are signaling extracellular organelles that modulate the tumor microenvironment and promote angiogenesis and tumor progression. This study aimed to explore the mechanism by which glioma-derived exosomes affect angiogenesis.qRT-PCR was used to determine the expression level of linc-POU3F3 in glioma tissue as well as glioma cell lines. Ultrafiltration combined with a purification method was used to isolate exosomes derived from A172 cells (A172-Exo) and linc-POU3F3 shRNA-treated A172 cells (shA172-Exo). Transmission electron microscopy, Western blot and tunable resistive pulse sensing (TRPS) were used to identify exosomes. In vitro migration, proliferation, and tube formation experiments, as well as in vivo CAM assays, were used to analyze the pro-angiogenesis ability of exosomes. qRT-PCR and Western blot were used to identify expression levels of angiogenesis-related genes and proteins in human brain microvascular endothelial cells (HBMECs) after being cultured with exosomes.The levels of linc-POU3F3 were upregulated in glioma tissue and significantly correlated with the advanced tumor stage. A172 cells exhibited the highest expression level. A172-Exo was similar to shA172-Exo (50-100 nm in diameter) and expressed Alix, Tsg101 and CD9, while the expression level of linc-POU3F3 in A172-Exo was significantly higher than that in shA172-Exo. HBMECs rapidly internalized A172-Exo and shA172-Exo, and the linc-POU3F3 expression level in HBMECs treated with A172-Exo was significantly higher than the level in HBMECs treated with shA172-Exo. A172-Exo exhibited better function in promoting HBMECs migration, proliferation, tubular-like structure formation in vitro and arteriole formation in vivo. The gene and protein expression level of bFGF, bFGFR, VEGFA, and Angio in HBMECs treated with A172-Exo was much higher than that of HBMECs treated with shA172-Exo.These results indicated that gliomas can induce angiogenesis by secreting exosomes enriched in linc-POU3F3. Exosomes and lncRNA-POU3F3 may, therefore, function as a putative therapeutic target in glioma.

Colorectal cancer (CRC), one of the most common human malignancies, is a major public health problem in the developed world. Capsaicin, widely used as a food additive and as an analgesic agent, is a major pungent ingredient of red pepper. Though capsaicin-induced apoptosis was previously reported in cancer cells, relatively little is known about the impact of capsaicin on other aspect of cancer cell behavior. In this study, we demonstrated that treatment with high-concentration of capsaicin (≥ 200 µM for SW480 and CT-26 cell lines; ≥ 25 µM for HCT116 cell line) inhibited CRC cell proliferation in a dose-dependent manner. In spite of no anti-proliferative effect, notably, low-concentration of capsaicin (100 µM for SW480 and CT-26 cell lines; 12.5 µM for HCT116 cell line) enhanced both migratory and invasive capability of these cells, which was validated by both in vitro and in vivo model. Further, we showed that 100 µM capsaicin induced epithelial-to-mesenchymal (EMT), up-regulated expression of MMP-2 and MMP-9, and activated Akt/mTOR and STAT-3 pathways in SW480 cells. Finally, we showed that capsaicin-induced metastasis of CRC cells was mediated by modulating reactive oxygen species (ROS) production. Our findings are considered a significant step toward a better understanding of capsaicin-associated regulatory network on CRC cells.

To investigate efficacy and safety of artery chemoembolization combined with radiofrequency ablation for advanced non-small cell lung cancer.A total of 138 cases of Sichuan Cancer Hospital of patients with advanced non-small cell lung cancer were retrospectively analyzed from January 2011 to November 2014.All cases were divided into three groups, 61 cases were treated by artery chemoembolization (ACB group), 43 cases were treated by artery chemoembolization combined with radiofrequency ablation (Joint group), 34 cases were treated by radiofrequency ablation (RFA group). Recent efficiency, overall survival time, adverse reactions and complications of three groups were analyzed. The risk factors related to survival time of 138 patients were analyzed.The recent efficiency of ACB group, Joint group and RFA group were 73.8%(45/61), 93.0%(40/43), 85.3%(29/34), respectively, and the difference was statistically significant (χ(2)=18.499, P=0.005). 1, 2, 3-year survival rates of Joint group were 90.7%(39/43), 58.1%(25/43), 20.9%(9/43), respectively that were better than ACB group(57.4%(35/61), 24.6%(15/61), 11.5%(7/61)) and RFA group(58.8%(20/34), 32.4%(11/34), 11.8%(4/34)), (χ(2) value were respectively 8.242 and 4.934, P values were 0.004 and 0.026). Chemotherapy side effects and complications of ablation were of no significant difference (P0.05). Chemotherapy side effects and complications of ablation were of no significant difference (P0.05). The number of tumors, tumor size, TNM staging, eastern cooperative oncology group (ECOG) score were independent risk factors which impact on survival time for non-small cell lung cancer. The artery chemoembolization combined with radiofrequency ablation treatment can improve survival time of patients with advanced non-small cell lung cancer.Artery chemoembolization combined with radiofrequency ablation is a safe, effective, minimally-invasive treatment for advanced non-small cell lung cancer.

This paper introduces an innovative technology—grouted jetted precast concrete piling—that increases the efficiency of piling operations in coastal regions. The technology includes the following steps: (i) casting concrete piles factory-designed especially for jetting and grouting; (ii) jetting to drive the concrete piles with a crane on a floating ship or platform into soil; and (iii) grouting to enhance the sheet pile connections and to increase the pile bearing capacity. This technology was applied to a number of piling construction projects at the mouth of the Yellow River Delta in China, and this experience demonstrated that it is a robust, fast track, cost-effective, and environmentally friendly piling method.Key words: piling technique, sheet pile, jetting, retaining walls, coastal infrastructure, Yellow River.

Collisions of Au + Au have been studied at beam kinetic energies of 6.0, 8.0 and 10.8 GeV/nucleon at the AGS facility at Brookhaven National Laboratory. Particles emitted from the collisions were momentum analysed and identified in a magnetic spectrometer. Proton rapidity distributions are compared with those expected for isotropic emission from a stopped source and it is concluded that there is a substantial amount of transparency at all three beam energies even for the most central collisions. Strangeness production were studied via measurements of K, and spectra. All three indicate a strong enhancement of strangeness production relative to pp-collisions at similar centre-of-mass energies.

The effects of baryon stopping and its resulting energy deposition on the dynamics of Au + Au collisions at 6, 8 and 10.8 GeV/nucleon are explored with recent results from the AGS experiment E917. Current analyses of stopping, collective flow signals and HBT parameters are presented. Strangeness and anti-baryon production is examined using the yields of anti-lambdas and anti-protons.

The E866 experimental setup has been upgraded by addition of a forward spectrometer to handle the large multiplicities in central Au+Au collisions at AGS. Furthermore new global detectors have been added to gain insight into the reaction dynamics and enable studies of flow effects. The report presents preliminary rapidity distributions for protons and produced mesons in central collisions. The shape of m{sub t}-spectra of pions is discussed. Examples of the measurements which can be done with a new multiplicity array are presented.

The fabrication process, compressive strength and biocompatibility of porous beta-tricalcium phosphate (beta-TCP) ceramic scaffolds reinforced with 45P(2)O(5)-22CaO-25Na(2)O-8MgO bioglass (beta-TCP/BG) were investigated for their suitability as bone engineering materials. Porous beta-TCP/BG scaffolds with macropore sizes of 200-500 muicrom were prepared by coating porous polyurethane template with beta-TCP/BG slurry. The beta-TCP/BG scaffolds showed interconnected porous structures and exhibited enhanced mechanical properties to those pure beta-TCP scaffolds. In order to assess the effects of chemical composition of this bioglass on the behavior of osteoblasts cultured in vitro, porous scaffolds were immersed in simulated body fluid (SBF) for 2 weeks, and original specimens (without soaked in SBF) seeded with MC3T3-E1 were cultured for the same period. The ability of inducing apatite crystals in simulated body fluid and the attachment of osteoblasts were examined. Results suggest that apatite agglomerates are formed on the surface of the beta-TCP/BG scaffolds and its Ca/P molar ratio is approximately 1.42. Controlling the crystallization from the beta-TCP/BG matrix could influence the releasing speed of inorganic ions and further adjust the microenvironment of the solution around the beta-TCP/BG, which could improve the interaction between osteoblasts and the scaffolds.

Two-pion correlation functions are analyzed at mid-rapidity for three systems (14.6 A-GeV Si+Al, Si+Au, and 11.6 A-GeV Au+Au), seven distinct centrality conditions, and different kT bins in the range 0.1--0.5 GeV/c. Source reference frames are determined from fits to the Yano-Koonin source parameterization. Bertsch-Pratt radius parameters are shown to scale linearly with both number of projectile and total participants as obtained from a Glauber model calculation. A finite emission duration that increases linearly with system/centrality is also reported. The mT dependence of the Bertsch-Pratt radii is measured for the central Si+Au and Au+Au systems. The system/centrality dependence is investigated separately for both high and low mT regions.

An excitation function of proton rapidity distributions for different centralities is reported from AGS Experiment E917 for Au+Au collisions at 6, 8, and 10.8 GeV/nucleon. The rapidity distributions from peripheral collisions have a valley at midrapidity which smoothly change to distributions that peak at midrapidity for central collisions. The mean rapidity loss increases with increasing beam energy, whereas the fraction of protons consistent with isotropic emission from a thermal source at midrapidity decreases with increasing beam energy., Comment: 9 pages, 3 figures, 1 table replaced version has figures rotated

Charged kaon production has been measured in Si+Al and Si+Au collisions at 14.6 A GeV/c, and Au+Au collisions at 11.1 A GeV/c by Experiments 859 and 866 (the E--802 Collaboration) at the BNL AGS. Invariant transverse mass spectra and rapidity distributions for both K+ and K- are presented. The centrality dependence of rapidity-integrated kaon yields is studied. Strangeness enhancement is observed as an increase in the slope of the kaon yield with the total number of participants as well as the yield per participant. The enhancement starts with peripheral Si+Al and Si+Au collisions (relative to N+N) and appears to saturate for a moderate number of participating nucleons in Si+Au collisions. It is also observed to increase slowly with centrality in Au+Au collisions, to a level in the most central Au+Au collisions that is greater than that found in central Si+A collisions. The enhancement factor for $K^+$ production are 3.0 +-0.2(stat.) +-0.4(syst.) and 4.0 +-0.3(stat.) +-0.5(syst.), respectively, for the most central 7% Si+Au collisions and the most central 4% Au+Au collisions relative to N+N at the correponding beam energy., Comment: RevTeX style, 18 pages, 9 figures, 1 table. Minor changes. Phys. Rev. C in press

A cycle C in G is said to be locally geodesic at a vertex ν if for each vertex u on C, the distance between ν and u in C coincides with that in G. It will be shown that a self-centered graph of radius 2 contains a cycle of length 4 or 5 which is locally geodesic at each vertex and conversely that if the longest one among such cycles for each vertex of a block has length 4 then the block is self-centered and has radius 2.