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6 results on '"Fuller, Troy E"'

1. Characterization of Actinobacillus pleuropneumoniae riboflavin biosynthesis genes

Author

Fuller, Troy E. and Mulks, Martha H.

Subjects
Escherichia coli -- Analysis, Biosynthesis -- Research, Bacterial genetics -- Research, Vitamin B2 -- Research, Nucleotide sequence -- Analysis, Biological sciences
Abstract

Genetic identification, cloning and nucleotide sequence studies are performed on four genes of Actinobacillus pleuropneumoniae which played a crucial role in riboflavin biosynthesis. Results show that the genes are capable of specifying the production of large amounts of riboflavin in Escherichia coli and complementing several defined genetic mutations in riboflavin biosynthesis in the same bacterium.

Published
1995

2. The development and maintenance of the mononuclear phagocyte system of the chick is controlled by signals from the macrophage colony-stimulating factor (CSF1) receptor

Author

Garceau, Valerie, Balic, Adam, Garcia-Morales, Carla, Sauter, Kristin A, McGrew, Mike J, Smith, Jacqueline, Vervelde, Lonneke, Sherman, Adrian, Fuller, Troy E, Oliphant, Theodore, Shelley, John A, Tiwari, Raksha, Wilson, Thomas L, Chintoan-Uta, Cosmin, Burt, Dave W, Stevens, Mark P, Sang, Helen M, and Hume, David A

Subjects
embryonic structures
Abstract

BACKGROUND: Macrophages have many functions in development and homeostasis as well as innate immunity. Recent studies in mammals suggest that cells arising in the yolk sac give rise to self-renewing macrophage populations that persist in adult tissues. Macrophage proliferation and differentiation is controlled by macrophage colony-stimulating factor (CSF1) and interleukin 34 (IL34), both agonists of the CSF1 receptor (CSF1R). In the current manuscript we describe the origin, function and regulation of macrophages, and the role of CSF1R signalling during embryonic development, using the chick as a model.RESULTS: Based upon RNAseq comparison to bone marrow-derived macrophages (BMDM) grown in CSF1, we show that embryonic macrophages contribute around 2% of the total embryo RNA in day 7 chick embryos, and have similar gene expression profiles to BMDM. To explore the origins of embryonic and adult macrophages, we injected HH16 chick embryos with either yolk-sac derived blood cells, or bone marrow cells from EGFP(+) donors. In both cases, the transferred cells gave rise to large numbers of EGFP(+) tissue macrophages in the embryo. In the case of the yolk sac, these cells were not retained in hatched birds. Conversely, bone marrow EGFP(+) cells gave rise to tissue macrophages in all organs of adult birds, and regenerated CSF1-responsive marrow macrophage progenitors. Surprisingly, they did not contribute to any other hematopoietic lineage. To explore the role of CSF1 further, we injected embryonic or hatchling CSF1R-reporter transgenic birds with a novel chicken CSF1-Fc conjugate. In both cases, the treatment produced a large increase in macrophage numbers in all tissues examined. There were no apparent adverse effects of chCSF1-Fc on embryonic or post-hatch development, but there was an unexpected increase in bone density in the treated hatchlings.CONCLUSIONS: The data indicate that the yolk sac is not the major source of macrophages in adult birds, and that there is a macrophage-restricted, self-renewing progenitor cell in bone marrow. CSF1R is demonstrated to be limiting for macrophage development during development in ovo and post hatch. The chicken provides a novel and tractable model to study the development of the mononuclear phagocyte system and CSF1R signalling.

Published
2015
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3. The development and maintenance of the mononuclear phagocyte system of the chick is controlled by signals from the macrophage colony-stimulating factor receptor

Author

Garceau, Valerie, primary, Balic, Adam, additional, Garcia-Morales, Carla, additional, Sauter, Kristin A, additional, McGrew, Mike J, additional, Smith, Jacqueline, additional, Vervelde, Lonneke, additional, Sherman, Adrian, additional, Fuller, Troy E, additional, Oliphant, Theodore, additional, Shelley, John A, additional, Tiwari, Raksha, additional, Wilson, Thomas L, additional, Chintoan-Uta, Cosmin, additional, Burt, Dave W, additional, Stevens, Mark P, additional, Sang, Helen M, additional, and Hume, David A, additional

Published
2015
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5. Correlation of lactulose-to-mannitol ratios in plasma and urine for intestinal permeability assessment in pigs.

Author

Hu, Steven X., Benner, Charles P., and Fuller, Troy E.

Subjects
*ORAL drug administration, *LIQUID chromatography-mass spectrometry, *PERMEABILITY, *STATISTICAL correlation, *LACTULOSE, *URINE
Abstract

OBJECTIVE The lactulose-to-mannitol ratio test is a test to assess the disorders associated with gut permeability. The test requires an oral administration of the mixture of lactulose and mannitol and urine collection. The urinary ratio of lactulose to mannitol is an indicator of intestinal permeability. Due to the complexity of urine collection in animal studies, plasma exposure ratios of lactulose to mannitol compared to their urinary concentration ratios were evaluated following an oral administration of the sugar mixture in pigs. ANIMALS 10 pigs were orally dosed with a solution of lactulose and mannitol mixture. PROCEDURES Plasma samples were collected at predose, 10 and 30 minutes and 2, 4, and 6 hours postdosing, and cumulated urinary samples were collected at 6 hours for liquid chromatography-mass spectrometry analysis. The ratios of pharmacokinetic parameters of lactulose to mannitol and the plasma sugar ratios at a single time point or the mean values of several time points were compared to their urinary sugar ratios. RESULTS The results revealed that the lactulose-to-mannitol ratios of AUC0-6h, AUCextrap, and Cmax were correlated to the urinary sugar ratios, and the plasma sugar ratios of a single time point at 2, 4, or 6 hours and the mean values of those time points were also appropriate to replace their urinary ratios in pigs. CLINICAL RELEVANCE Following an oral administration of lactulose and mannitol mixture, blood collection, and assay can be an option for assessing intestinal permeability, especially in animal studies. [ABSTRACT FROM AUTHOR]

Published
2023
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6. The development and maintenance of the mononuclear phagocyte system of the chick is controlled by signals from the macrophage colony-stimulating factor receptor

Author

Garceau, Valerie, Balic, Adam, Garcia-Morales, Carla, Sauter, Kristin A, McGrew, Mike J, Smith, Jacqueline, Vervelde, Lonneke, Sherman, Adrian, Fuller, Troy E, Oliphant, Theodore, Shelley, John A, Tiwari, Raksha, Wilson, Thomas L, Chintoan-Uta, Cosmin, Burt, Dave W, Stevens, Mark P, Sang, Helen M, and Hume, David A

Subjects
animal structures, Agricultural and Biological Sciences(all), Biochemistry, Genetics and Molecular Biology(all), embryonic structures
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