Introduction: Angiostrongylus vasorum is a nematode that parasitizes molluscs, dogs, and even man., Methods: The objective was to evaluate the predatory activity of the conidia of two fungal isolates of Duddingtonia flagrans (AC001 and CG722) on first-stage larvae (L1) of A. vasorum in laboratory conditions., Results: At the end of the experiment, there were significant reductions (p<0.01) of 74.5% and 63.2%, on average, in the A. vasorum L1 recovered in the AC001 and CG722 treatment conditions, respectively., Conclusions: The two isolates of fungi were efficient in the capture and destruction of A. vasorum L1.