Search

Your search keyword '"Fracchiolla, Giuseppe"' showing total 33 results
Start Over Author "Fracchiolla, Giuseppe" Search Limiters Available in Library Collection
33 results on '"Fracchiolla, Giuseppe"'

2. Dopamine- and Grape-Seed-Extract-Loaded Solid Lipid Nanoparticles: Interaction Studies between Particles and Differentiated SH-SY5Y Neuronal Cell Model of Parkinson’s Disease

Author

Mallamaci, Rosanna, primary, Musarò, Debora, additional, Greco, Marco, additional, Caponio, Antonello, additional, Castellani, Stefano, additional, Munir, Anas, additional, Guerra, Lorenzo, additional, Damato, Marina, additional, Fracchiolla, Giuseppe, additional, Coppola, Chiara, additional, Cardone, Rosa Angela, additional, Rashidi, Mehdi, additional, Tardugno, Roberta, additional, Sergio, Sara, additional, Trapani, Adriana, additional, and Maffia, Michele, additional

Published
2024
Full Text
View/download PDF

3. In Vitro Virucidal Activity of Different Essential Oils against Bovine Viral Diarrhea Virus Used as Surrogate of Human Hepatitis C Virus.

Author

Lanave, Gianvito, Pellegrini, Francesco, Triggiano, Francesco, De Giglio, Osvalda, Lucente, Maria Stella, Diakoudi, Georgia, Catella, Cristiana, Gentile, Arturo, Tardugno, Roberta, Fracchiolla, Giuseppe, Martella, Vito, and Camero, Michele

Subjects
BOVINE viral diarrhea virus, HEPATITIS C virus, ESSENTIAL oils, LEMON balm, SAGE
Abstract

The hepatitis C virus (HCV) is a major hepatotropic virus that affects humans with increased risk of developing hepatocellular carcinoma. The bovine viral diarrhea virus (BVDV) causes abortion, calf mortality and poor reproductive performance in cattle. Due the difficulties of in vitro cultivation for HCV, BVDV has been used as surrogate for in vitro assessment of the efficacy of antivirals. Essential oils (EOs) display antiviral and virucidal activity on several viral pathogens. In this study, the virucidal activity of five EOs, Salvia officinalis L. EO (SEO), Melissa officinalis L. EO (MEO), Citrus lemon EO (LEO), Rosmarinus officinalis L. EO (REO) and Thymus vulgaris L. EO (TEO) against BVDV was evaluated in vitro at different concentrations for several time contacts. MEO and LEO were able to considerably inactivate BVDV with a time- and dose-dependent fashion. MEO and LEO at the highest concentrations decreased viral titer by 2.00 and 2.25 log10 TCID50/50 μL at 8 h contact time, respectively. SEO, REO and TEO displayed mild virucidal activity at the highest concentrations for 8 h contact times. In this study, the virucidal efficacies of MEO and LEO against BVDV were observed regardless of compound concentration and contact time. Further studies are needed to confirm the potential use of MEO and LEO as surface disinfectants. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

6. Solid Lipid Nanoparticles Containing Dopamine and Grape Seed Extract: Freeze-Drying with Cryoprotection as a Formulation Strategy to Achieve Nasal Powders

Author

De Giglio, Elvira, primary, Bakowsky, Udo, additional, Engelhardt, Konrad, additional, Caponio, Antonello, additional, La Pietra, Matteo, additional, Cometa, Stefania, additional, Castellani, Stefano, additional, Guerra, Lorenzo, additional, Fracchiolla, Giuseppe, additional, Poeta, Maria Luana, additional, Mallamaci, Rosanna, additional, Cardone, Rosa Angela, additional, Bellucci, Stefano, additional, and Trapani, Adriana, additional

Published
2023
Full Text
View/download PDF

7. Combined Dopamine and Grape Seed Extract-Loaded Solid Lipid Nanoparticles: Nasal Mucosa Permeation, and Uptake by Olfactory Ensheathing Cells and Neuronal SH-SY5Y Cells

Author

Trapani, Adriana, primary, Castellani, Stefano, additional, Guerra, Lorenzo, additional, De Giglio, Elvira, additional, Fracchiolla, Giuseppe, additional, Corbo, Filomena, additional, Cioffi, Nicola, additional, Passantino, Giuseppe, additional, Poeta, Maria Luana, additional, Montemurro, Pasqualina, additional, Mallamaci, Rosanna, additional, Cardone, Rosa Angela, additional, and Conese, Massimo, additional

Published
2023
Full Text
View/download PDF

8. Pilot Study on the Action of Thymus vulgaris Essential Oil in Treating the Most Common Bacterial Contaminants and Salmonella enterica subsp. enterica Serovar Derby in Poultry Litter

Author

Galgano, Michela, primary, Pellegrini, Francesco, additional, Fracchiolla, Giuseppe, additional, Mrenoshki, Daniela, additional, Zarea, Aya Attia Koraney, additional, Bianco, Angelica, additional, Del Sambro, Laura, additional, Capozzi, Loredana, additional, Schiavone, Antonella, additional, Saleh, Medhat S., additional, Camero, Michele, additional, Tempesta, Maria, additional, Cirone, Francesco, additional, Buonavoglia, Domenico, additional, Pratelli, Annamaria, additional, and Buonavoglia, Alessio, additional

Published
2023
Full Text
View/download PDF

9. Virucidal Activity of Lemon Essential Oil against Feline Calicivirus Used as Surrogate for Norovirus

Author

Pellegrini, Francesco, primary, Camero, Michele, additional, Catella, Cristiana, additional, Fracchiolla, Giuseppe, additional, Sblano, Sabina, additional, Patruno, Giovanni, additional, Trombetta, Claudia Maria, additional, Galgano, Michela, additional, Pratelli, Annamaria, additional, Tempesta, Maria, additional, Martella, Vito, additional, and Lanave, Gianvito, additional

Published
2023
Full Text
View/download PDF

11. Antimicrobial Activity of Essential Oils Evaluated In Vitro against Escherichia coli and Staphylococcus aureus

Author

Galgano, Michela, primary, Capozza, Paolo, additional, Pellegrini, Francesco, additional, Cordisco, Marco, additional, Sposato, Alessio, additional, Sblano, Sabina, additional, Camero, Michele, additional, Lanave, Gianvito, additional, Fracchiolla, Giuseppe, additional, Corrente, Marialaura, additional, Cirone, Francesco, additional, Trotta, Adriana, additional, Tempesta, Maria, additional, Buonavoglia, Domenico, additional, and Pratelli, Annamaria, additional

Published
2022
Full Text
View/download PDF

23. Kidney CLC-K Chloride Channels Show Differential Pharmacological Profiles Depending on the Heterologous Expression System

Author

Liantonio, Antonella, primary, Imbrici, Paola, additional, Gramegna, Gianluca, additional, Gradogna, Antonella, additional, Zifarelli, Giovanni, additional, Laghezza, Antonio, additional, Fracchiolla, Giuseppe, additional, Carbonara, Giuseppe, additional, Loiodice, Fulvio, additional, Pusch, Michael, additional, and Conte Camerino, Diana, additional

Published
2013
Full Text
View/download PDF

25. Molecular switch for CLC-K Cl − channel block/activation: Optimal pharmacophoric requirements towards high-affinity ligands

Author

Liantonio, Antonella, primary, Picollo, Alessandra, additional, Carbonara, Giuseppe, additional, Fracchiolla, Giuseppe, additional, Tortorella, Paolo, additional, Loiodice, Fulvio, additional, Laghezza, Antonio, additional, Babini, Elena, additional, Zifarelli, Giovanni, additional, Pusch, Michael, additional, and Camerino, Diana Conte, additional

Published
2008
Full Text
View/download PDF

26. Insights into the Mechanism of Partial Agonism

Author

Pochetti, Giorgio, primary, Godio, Cristina, additional, Mitro, Nico, additional, Caruso, Donatella, additional, Galmozzi, Andrea, additional, Scurati, Samuele, additional, Loiodice, Fulvio, additional, Fracchiolla, Giuseppe, additional, Tortorella, Paolo, additional, Laghezza, Antonio, additional, Lavecchia, Antonio, additional, Novellino, Ettore, additional, Mazza, Fernando, additional, and Crestani, Maurizio, additional

Published
2007
Full Text
View/download PDF

28. Structural requisites of 2-(p -chlorophenoxy)propionic acid analogues for activity on native rat skeletal muscle chloride conductance and on heterologously expressed CLC-1

Author

Liantonio, Antonella, primary, De Luca, Annamaria, additional, Pierno, Sabata, additional, Didonna, Maria Paola, additional, Loiodice, Fulvio, additional, Fracchiolla, Giuseppe, additional, Tortorella, Paolo, additional, Laghezza, Antonio, additional, Bonerba, Elisabetta, additional, Traverso, Sonia, additional, Elia, Laura, additional, Picollo, Alessandra, additional, Pusch, Michael, additional, and Camerino, Diana Conte, additional

Published
2003
Full Text
View/download PDF

29. Molecular switch for CLC-K Cl- channel block/activation: Optimal pharmacophoric requirements towards high-affinity ligands.

Author

Liantonio, Antonella, Picollo, Alessandra, Carbonara, Giuseppe, Fracchiolla, Giuseppe, Tortorella, Paolo, Loiodice, Fulvio, Laghezza, Antonio, Babini, Elena, Zifarelli, Giovanni, Pusch, Michael, and Camerino, Diana Conte

Subjects
LIGANDS (Biochemistry), IMMOBILIZED ligands (Biochemistry), BIOCHEMISTRY, TARGETED drug delivery, DRUG development, PROPIONIC acid
Abstract

CIC-Ka and CIC-Kb Cl- channels are pivotal for renal salt reabsorption and water balance. There is growing interest in identifying ligands that allow pharmacological interventions aimed to modulate their activity. Starting from available ligands, we followed a rational chemical strategy, accompanied by computational modeling and electrophysiological techniques, to identify the molecular requisites for binding to a blocking or to an activating binding site on CIC-Ka. The major molecular determinant that distinguishes activators from blockers is the level of planarity of the aromatic portions of the molecules: only molecules with perfectly coplanar aromatic groups display potentiating activity. Combining several molecular features of various CLC-K ligands. we discovered that phenyl-benzofuran carboxylic acid derivatives yield the most potent CIC-Ka inhibitors so far described (affinity <10 μM). The increase in affinity compared with 3-phenyl-2-p-chlorophenoxy-propionic acid (3-phenyl-CPP) stems primarily from the conformational constraint provided by the phenyl-benzofuran ring. Several other key structural elements for high blocking potency were identified through a detailed structure-activity relationship study. Surprisingly, some benzofuran-based drugs inhibit did-Kb with a similar affinity of <10 μM, thus representing the first inhibitors for this CLC-K isoform identified so far. Based on our data, we established a pharmacophore model that will be useful for the development of drugs targeting CLC-K channels. [ABSTRACT FROM AUTHOR]

Published
2008
Full Text
View/download PDF

30. Structural requisites of 2-(p-chlorophenoxy) propionic acid analogues for activity on native rat skeletal muscle chloride conductance and on heterologously expressed CLC-1.

Author

Liantonio, Antonella, De Luca, Annamaria, Pierno, Sabata, Didonna, Maria Paola, Loiodice, Fulvio, Fracchiolla, Giuseppe, Tortorella, Paolo, Laghezza, Antonio, Bonerba, Elisabetta, Traverso, Sonia, Elia, Laura, Picollo, Alessandra, Pusch, Michael, and Camerino, Diana Conte

Subjects
PROPIONIC acid, CHLORIDE channels, MUSCLES
Abstract

Discusses the structure of propionic 2-(p-chlorophenoxy)propionic acid (CPP) analogues for activity in the skeletal muscle chloride conductance of rats. Independent modification of CCP structure leading to a decrease of chloride blocking activity; Identification of four molecular determinants pivotal to the interaction with the binding site on muscle CLC-1 channels.

Published
2003
Full Text
View/download PDF

31. Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture

Author

Adriana Trapani, María Ángeles Esteban, Francesca Curci, Daniela Erminia Manno, Antonio Serra, Giuseppe Fracchiolla, Cristóbal Espinosa-Ruiz, Stefano Castellani, Massimo Conese, Trapani, Adriana, Ángeles Esteban, María, Curci, Francesca, Manno, Daniela Erminia, Serra, Antonio, Fracchiolla, Giuseppe, Espinosa-Ruiz, Cristóbal, Castellani, Stefano, and Conese, Massimo

Subjects
Fish Proteins, Polyphenol, solid lipid nanoparticles, grape seed–derived extract, drug delivery, X-ray diffraction, antioxidant activity, fish cells, Pharmaceutical Science, Organic chemistry, solid lipid nanoparticle, Aquaculture, Article, Antioxidants, Analytical Chemistry, QD241-441, Nanoparticle, Drug Discovery, Animals, Vitis, Physical and Theoretical Chemistry, Grape Seed Extract, Animal, Fishes, Polyphenols, fish cell, Oxidative Stre, Liposome, Oxidative Stress, Gene Expression Regulation, Chemistry (miscellaneous), Fish Protein, Liposomes, Molecular Medicine, Nanoparticles, Antioxidant, Fishe
Abstract

The supply of nutrients, such as antioxidant agents, to fish cells still represents a challenge in aquaculture. In this context, we investigated solid lipid nanoparticles (SLN) composed of a combination of Gelucire® 50/13 and Precirol® ATO5 to administer a grape seed extract (GSE) mixture containing several antioxidant compounds. The combination of the two lipids for the SLN formation resulted in colloids exhibiting mean particle sizes in the range 139–283 nm and zeta potential values in the range +25.6–43.4 mV. Raman spectra and X-ray diffraction evidenced structural differences between the free GSE and GSE-loaded SLN, leading to the conclusion that GSE alters the structure of the lipid nanocarriers. From a biological viewpoint, cell lines from gilthead seabream and European sea bass were exposed to different concentrations of GSE-SLN for 24 h. In general, at appropriate concentrations, GSE-SLN increased the viability of the fish cells. Furthermore, regarding the gene expression in those cells, the expression of antioxidant genes was upregulated, whereas the expression of hsp70 and other genes related to the cytoskeleton was downregulated. Hence, an SLN formulation containing Gelucire® 50/13/Precirol® ATO5 and GSE may represent a compelling platform for improving the viability and antioxidant properties of fish cells.

Published
2022

32. Comparative analysis of enantioselective separation of novel PPAR agonists by HPLC on cellulose- and amylose-based chiral stationary phases.

Author

Piemontese, Luca, Faliti, Salvatore, Carbonara, Giuseppe, Fracchiolla, Giuseppe, Tortorella, Paolo, and Loiodice, Fulvio

Subjects
*CHIRALITY, *POLYCYCLIC compounds, *PROPIONIC acid, *ADIPIC acid, *ENANTIOMERS, *HIGH density lipoproteins, *TRIGLYCERIDES, *NUCLEAR receptors (Biochemistry), *CHROMATOGRAPHIC analysis
Abstract

Chiral chromatographic resolution of a series of dual PPARα/γ agonists was performed on the commercially available Chiralcel OD and Chiralpak AD columns in normal phase mode using a mobile phase consisting of a mixture of n-hexane, 2-propanol and trifluoroacetic acid at constant volume ratio. The selected CSPs resolved most compounds as underivatized acids without requiring time consuming analysis. Both electronic features and steric hindrance affected chiral interactions between the analytes and the CSPs. Additional information on the chiral recognition mechanism was deduced from the chromatographic behaviour of some selected methyl esters. [ABSTRACT FROM AUTHOR]

Published
2009

33. Exploring the Antibiofilm Effect of Sertraline in Synergy with Cinnamomum verum Essential Oil to Counteract Candida Species.

Author

Barbarossa A, Rosato A, Carrieri A, Fumarola L, Tardugno R, Corbo F, Fracchiolla G, and Carocci A

Abstract

The emergence and spread of drug-resistant pathogens, resulting in antimicrobial resistance, continue to compromise our capability to handle commonly occurring infectious diseases. The rapid global spread of multi-drug-resistant pathogens, particularly systemic fungal infections, presents a significant concern, as existing antimicrobial drugs are becoming ineffective against them. In recent decades, there has been a notable increase in systemic fungal infections, primarily caused by Candida species, which are progressively developing resistance to azoles. Moreover, Candida species biofilms are among the most common in clinical settings. In particular, they adhere to biomedical devices, growing as a resilient biofilm capable of withstanding extraordinarily high antifungal concentrations. In recent years, many research programs have concentrated on the development of novel compounds with possible antimicrobial effects to address this issue, and new sources, such as plant-derived antimicrobial compounds, have been thoroughly investigated. Essential oils (EOs), among their numerous pharmacological properties, exhibit antifungal, antibacterial, and antiviral activities and have been examined at a global scale as the possible origin of novel antimicrobial compounds. A recent work carried out by our research group concerned the synergistic antibacterial activities of commercially available and chemically characterized Cinnamomum verum L. essential oil ( C. verum EO) in association with sertraline, a selective serotonin reuptake inhibitor whose repositioning as a non-antibiotic drug has been explored over the years with encouraging results. The aim of this work was to explore the synergistic effects of C. verum EO with sertraline on both planktonic and sessile Candida species cells. Susceptibility testing and testing of the synergism of sertraline and C. verum EO against planktonic and sessile cells were performed using a broth microdilution assay and checkerboard methods. A synergistic effect was evident in both the planktonic cells and mature biofilms, with significant reductions in fungal viability. Indeed, the fractional inhibitory concentration index (FICI) was lower than 0.5 for all the associations, thus indicating significant synergism of the associations with the Candida strains examined. Moreover, the concentrations of sertraline able to inhibit Candida spp. strain growth and biofilm formation significantly decreased when it was used in combination with C. verum EO for all the strains considered, with a reduction percentage in the amount of each associated component ranging from 87.5% to 97%.

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results