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Single-cell genomics is a powerful tool for studying heterogeneous tissues such as the brain. Yet little is understood about how genetic variants influence cell-level gene expression. Addressing this, we uniformly processed single-nuclei, multiomics datasets into a resource comprising >2.8 million nuclei from the prefrontal cortex across 388 individuals. For 28 cell types, we assessed population-level variation in expression and chromatin across gene families and drug targets. We identified >550,000 cell type-specific regulatory elements and >1.4 million single-cell expression quantitative trait loci, which we used to build cell-type regulatory and cell-to-cell communication networks. These networks manifest cellular changes in aging and neuropsychiatric disorders. We further constructed an integrative model accurately imputing single-cell expression and simulating perturbations; the model prioritized ~250 disease-risk genes and drug targets with associated cell types.

Rural residents are at a disadvantage with regard to health status and access to health promotion activities. In many rural communities, public libraries offer support through health information provision; there are also opportunities for engagement in broader community health efforts. In a collaborative effort between an academic researcher and a rural public library director in North Carolina, pedometers and an individualized online health self-assessment program were made available to library users. The activities were adopted enthusiastically. Feedback included encouragement for more such opportunities; some claimed the activities improved awareness and supported healthy behavior change. With minimal investment, rural public libraries can support healthy lifestyle activities. They also afford a unique environment where library staff can collaborate with researchers to introduce, assess and evaluate community health initiatives. As we train future practitioners, we should embrace the opportunities that rural public libraries can provide for collaborative initiatives to invoke positive community change.

To better understand health information provision in the public library setting, two cooperative library systems that serve primarily rural populations in upstate New York were studied. The central library in one of those systems established a consumer health information center (CHIC) in 1999. In the other system, the central library does not have such a center. Visits were made to ten randomly selected libraries in each of the two systems (n = 20), and the query "Do vaccines cause autism?" was posed to library staff. Print resources were provided more often than online resources in both systems. Utilization of the CHIC as a health information resource was inconsistent among libraries. Follow-up phone interviews found none of the twenty libraries had reference policies. Reported practice matched actual practice 60 percent of the time in the system without the CHIC and 40 percent of the time in the system with the CHIC. (Contains 5 tables.)

Byline: Ramnath. Ramanathan, Gayathri. Sreedher, Konark. Malhotra, Zain. Guduru, Deeksha. Agarwal, Mary. Flaherty, Timothy. Leichliter, Sandeep. Rana Stroke-like migraine attacks after radiation therapy (SMART) syndrome is a rare delayed complication [...]