22 results on '"Fidalgo, Luis Eusebio"'
Search Results
2. Mapping the risk of exposure to Crimean-Congo haemorrhagic fever virus in the Iberian Peninsula using Eurasian wild boar (Sus scrofa) as a model
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Junta de Comunidades de Castilla-La Mancha, European Commission, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Instituto de Salud Carlos III, Universidad de Castilla La Mancha, Universidad de Córdoba (España), Universidad de Extremadura, Eusko Jaurlaritza, Baz-Flores, Sara, Herraiz, Cesar, Peralbo-Moreno, Alfonso, Barral, Marta, Arnal, Maria Cruz, Balseiro, Ana, Cano-Terriza, David, Castro-Scholten, Sabrina, Cevidanes, Aitor, Conde-Lizarralde, Alazne, Cuadrado-Matías, Raúl, Escribano, Fernando, Fernández de Luco, Daniel, Fidalgo, Luis Eusebio, Hermoso-de Mendoza, Javier, Fandos, Paulino, Gómez-Guillamón, Félix, Granados, José E., Jiménez-Martín, Débora, López-Olvera, Jorge R., Martín, Inés, Martínez, Remigio, Mentaberre, Gregorio, García-Bocanegra, Ignacio, Ruiz-Fons, Francisco, Junta de Comunidades de Castilla-La Mancha, European Commission, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Instituto de Salud Carlos III, Universidad de Castilla La Mancha, Universidad de Córdoba (España), Universidad de Extremadura, Eusko Jaurlaritza, Baz-Flores, Sara, Herraiz, Cesar, Peralbo-Moreno, Alfonso, Barral, Marta, Arnal, Maria Cruz, Balseiro, Ana, Cano-Terriza, David, Castro-Scholten, Sabrina, Cevidanes, Aitor, Conde-Lizarralde, Alazne, Cuadrado-Matías, Raúl, Escribano, Fernando, Fernández de Luco, Daniel, Fidalgo, Luis Eusebio, Hermoso-de Mendoza, Javier, Fandos, Paulino, Gómez-Guillamón, Félix, Granados, José E., Jiménez-Martín, Débora, López-Olvera, Jorge R., Martín, Inés, Martínez, Remigio, Mentaberre, Gregorio, García-Bocanegra, Ignacio, and Ruiz-Fons, Francisco
- Abstract
Crimean-Congo haemorrhagic fever (CCHF) virus (CCHFV) is a tick-borne zoonotic pathogen that can cause a lethal haemorrhagic disease in humans. Although the virus appears to be endemically established in the Iberian Peninsula, CCHF is an emerging disease in Spain. Clinical signs of CCHFV infection are mainly manifested in humans, but the virus replicates in several animal species. Understanding the determinants of CCHFV exposure risk from animal models is essential to predicting high-risk exposure hotspots for public health action. With this objective in mind, we designed a cross-sectional study of Eurasian wild boar (Sus scrofa) in Spain and Portugal. The study analysed 5,291 sera collected between 2006 and 2022 from 90 wild boar populations with a specific double-antigen ELISA to estimate CCHFV serum prevalence and identify the main determinants of exposure probability. To do so, we statistically modelled exposure risk with host- and environment-related predictors and spatially projected it at a 10 × 10 km square resolution at the scale of the Iberian Peninsula to map foci of infection risk. Fifty-seven (63.3 %) of the 90 populations had at least one seropositive animal, with seroprevalence ranging from 0.0 to 88.2 %. Anti-CCHFV antibodies were found in 1,026 of 5,291 wild boar (19.4 %; 95 % confidence interval: 18.3–20.5 %), with highest exposure rates in southwestern Iberia. The most relevant predictors of virus exposure risk were wild boar abundance, local rainfall regime, shrub cover, winter air temperature and soil temperature variation. The spatial projection of the best-fit model identified high-risk foci as occurring in most of western and southwestern Iberia and identified recently confirmed risk foci in eastern Spain. The results of the study demonstrate that serological surveys of CCHFV vector hosts are a powerful, robust and highly informative tool for public health authorities to take action to prevent human cases of CCHF in enzootic and emergency set
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- 2024
3. Biochemical Effects of Heavy Metals and Organochlorine Compounds Accumulated in Different Tissues of Yellow-Legged Gulls (Larus Michahellis)
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Junta de Extremadura, Vizuete, Jorge [0009-0004-6859-1801], Pérez-López, Marcos [0000-0003-2823-1671], Fidalgo, Luis Eusebio [0000-0001-9184-0942], Míguez-Santiyán, María Prado [0000-0002-7700-6713], Hernández Moreno, David [0000-0002-1189-9299], Vizuete, Jorge, Pérez-López, Marcos, López-Beceiro, Ana, Fidalgo, Luis Eusebio, Soler, Francisco, Míguez-Santiyán, María Prado, Hernández Moreno, David, Junta de Extremadura, Vizuete, Jorge [0009-0004-6859-1801], Pérez-López, Marcos [0000-0003-2823-1671], Fidalgo, Luis Eusebio [0000-0001-9184-0942], Míguez-Santiyán, María Prado [0000-0002-7700-6713], Hernández Moreno, David [0000-0002-1189-9299], Vizuete, Jorge, Pérez-López, Marcos, López-Beceiro, Ana, Fidalgo, Luis Eusebio, Soler, Francisco, Míguez-Santiyán, María Prado, and Hernández Moreno, David
- Abstract
In the present study, livers, kidneys and adipose tissue of Yellow-legged Gull (Larus michahellis) were collected. Samples were used to determine relationships between heavy metals/metalloids in liver and kidneys (Hg, Cd, Pb, Se and As) or persistent organic pollutants in adipose tissue (7 PCBs and 11 organochlorine pesticides) with biomarkers of oxidative stress (CAT, GPx, GR, GSH, GST, MDA) analysed in both internal organs. Three possible influencing variables have been studied: age, sex and sampling area. As a result, statistically significant differences (P < 0.05, P < 0.01) were only found according to the sampling area, with differences among the three studied areas found in both organs. Significant positive correlations (P < 0.01) were found in liver (Hg vs. GST; Se vs. MDA) and in kidney (As vs. GR; As vs. GPx; PCB52 vs. CAT; PCB138 vs. CAT). The scarcity in correlations suggests that the levels of pollutants found in animals were not high enough to trigger an effect at the oxidative level.
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- 2023
4. Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation
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Fundación Biodiversidad, Ministerio para la Transición Ecológica y el Reto Demográfico (España), Castaño, Cristina [0000-0003-1134-1436], Toledano Díaz, Adolfo [0000-0001-6679-485X], Caamaño, José Néstor [0000-0001-6449-5784], Fidalgo, Luis Eusebio [0000-0001-9184-0942], Esteso, Milagros C. [0000-0002-8963-5736], García-Casado, Pedro [0000-0002-1242-1035], Lukaszewicz, Ewa [0000-0002-9505-0056], Santiago Moreno, Julián [0000-0001-5551-8120], Hidalgo, Carlos Olegario [0000-0002-9810-1517], O'Brien, Emma, Castaño, Cristina, Toledano-Díaz, A., Caamaño, J. N., Hidalgo, Carlos Olegario, Fidalgo, Luis Eusebio, López-Beceiro, Ana, Esteso, Milagros C., Balsera, Ramón, García-Casado, Pedro, Lukaszewicz, Ewa, Santiago Moreno, Julián, Fundación Biodiversidad, Ministerio para la Transición Ecológica y el Reto Demográfico (España), Castaño, Cristina [0000-0003-1134-1436], Toledano Díaz, Adolfo [0000-0001-6679-485X], Caamaño, José Néstor [0000-0001-6449-5784], Fidalgo, Luis Eusebio [0000-0001-9184-0942], Esteso, Milagros C. [0000-0002-8963-5736], García-Casado, Pedro [0000-0002-1242-1035], Lukaszewicz, Ewa [0000-0002-9505-0056], Santiago Moreno, Julián [0000-0001-5551-8120], Hidalgo, Carlos Olegario [0000-0002-9810-1517], O'Brien, Emma, Castaño, Cristina, Toledano-Díaz, A., Caamaño, J. N., Hidalgo, Carlos Olegario, Fidalgo, Luis Eusebio, López-Beceiro, Ana, Esteso, Milagros C., Balsera, Ramón, García-Casado, Pedro, Lukaszewicz, Ewa, and Santiago Moreno, Julián
- Abstract
The Cantabrian capercaillie (Tetrao urogallus cantabricus) is critically endangered. This subspecies has the lowest genetic variability and it is in regression. It belongs to Phasianidae family; therefore, the domestic chicken (Gallus gallus domesticus) could be a good model for developing reproductive technologies for use in capercaillie populations with low availability of animals.
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- 2022
5. The role of healthy dog carriers of Babesia microti-like piroplasms
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Checa, Rocío, Fidalgo, Luis Eusebio, Montoya, Ana, López, Ana María, Barrera, Juan Pedro, Gálvez, Rosa, Sánchez de la Nieta, Sara, Marino, Valentina, Sarquis, Juliana, and Miró, Guadalupe
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- 2019
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6. The olfactory limbus of the red fox (Vulpes vulpes). New insights regarding a noncanonical olfactory bulb pathway
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Ortiz-Leal, Irene, primary, Torres, Mateo V., additional, Vargas-Barroso, Víctor, additional, Fidalgo, Luis Eusebio, additional, López-Beceiro, Ana María, additional, Larriva-Sahd, Jorge A., additional, and Sánchez-Quinteiro, Pablo, additional
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- 2023
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7. Heavy Metals Content in Great Shearwater (Ardenna Gravis): Accumulation, Distribution and Biomarkers of Effect in Different Tissues
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Universidad de Santiago de Compostela, Hernández Moreno, David [0000-0002-1189-9299], Ramos, Atocha [0000-0001-8356-241X], Romay, Cosme Damián [0000-0002-2735-2239], Fidalgo, Luis Eusebio [0000-0001-9184-0942], Menozzi, Alessandro [0000-0003-2957-0018], Hernández Moreno, David, Ramos, Atocha, Romay, Cosme Damián, Fidalgo, Luis Eusebio, Menozzi, Alessandro, Bertini, Simonea, Universidad de Santiago de Compostela, Hernández Moreno, David [0000-0002-1189-9299], Ramos, Atocha [0000-0001-8356-241X], Romay, Cosme Damián [0000-0002-2735-2239], Fidalgo, Luis Eusebio [0000-0001-9184-0942], Menozzi, Alessandro [0000-0003-2957-0018], Hernández Moreno, David, Ramos, Atocha, Romay, Cosme Damián, Fidalgo, Luis Eusebio, Menozzi, Alessandro, and Bertini, Simonea
- Abstract
The purpose of this study was to explore the usefulness of Great Shearwater (Ardenna gravis) as a bioindicator for biomonitoring programs for metal pollution. Three different metals were analysed in liver, kidney, and feathers, including cadmium, lead, and zinc. Glutathione-S-transferase, malondialdehyde, reduced glutathione, and catalase were assessed as oxidative stress biomarkers. Sex-related trends in metal accumulation also were evaluated. In liver and kidney, the mean concentrations of Zn (146.1 ± 5.14 and 108 ± 2.70 mg/kg, respectively) and Pb (0.19 ± 0.01 and 0.13 ± 0.01 mg/kg, respectively) in A. gravis were generally comparable to values reported in other studies. However, animals presented slightly higher concentrations of Cd (9.67 ± 0.65 in liver and 17.41 ± 0.84 mg/kg in kidney) than those reported in the same species sampled in Southern Atlantic waters. The slightly higher levels of Cd found in this study compared with other studies are probably affected by the location in Northern Atlantic waters (with different diet intake). In feathers, levels of Zn (70.70 ± 1.76 mg/kg) were lower than in other Ardenna shearwaters, whereas higher levels were found for Cd (0.16 ± 0.01 mg/kg) and Pb (0.84 ± 0.06 mg/kg). The lack of differences found between males and females could be influenced by the migration status, because both sexes stay in similar physiological conditions, with no laying eggs. Levels found in the feathers of the present study were related to concentrations in internal tissues below those which cause adverse effects in birds. Thus, feathers would appear as a potential noninvasive tool for metals biomonitoring in seabirds, because it is possible to quantify them. Baseline data of oxidative stress levels have been reported, both in liver and kidney, presenting no correlations with the levels of metals in these tissues. The low internal metal levels and the lack of correlations between oxidative stress metrics suggest a low risk of the environmental
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- 2021
8. A High Prevalence of Cardiopulmonary Worms Detected in the Iberian Wolf (Canis lupus): A Threat for Wild and Domestic Canids
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Estévez-Sánchez, Efrén, primary, Checa, Rocío, additional, Montoya, Ana, additional, Barrera, Juan Pedro, additional, López-Beceiro, Ana María, additional, Fidalgo, Luis Eusebio, additional, and Miró, Guadalupe, additional
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- 2022
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9. A High Prevalence of Cardiopulmonary Worms Detected in the Iberian Wolf (Canis lupus): A Threat for Wild and Domestic Canids
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Estévez Sánchez, Efrén, Checa Herráiz, Rocío, Montoya Matute, Ana, Barrera, Juan Pedro, López Beceiro, Ana María, Fidalgo, Luis Eusebio, Miró Corrales, Guadalupe, Estévez Sánchez, Efrén, Checa Herráiz, Rocío, Montoya Matute, Ana, Barrera, Juan Pedro, López Beceiro, Ana María, Fidalgo, Luis Eusebio, and Miró Corrales, Guadalupe
- Abstract
Cardiopulmonary nematodes are highly pathogenic parasites affecting domestic and wild canids. As the result of conservation programs, the Iberian wolf (Canis lupus signatus) population has recently expanded, and its distribution range covers lands from where it had long disappeared. However, the exact epidemiological role of the wolf in the life cycle of zoonotic parasites causing diseases transmissible to pets and/or humans is largely unknown. This study sought to determine the diversity of cardiopulmonary nematode parasite species that affect wolves inhabiting northwestern areas of the Iberian Peninsula, and to estimate their prevalence and the relationship between these parasites and several epidemiological variables. For this purpose, we examined the cardiopulmonary systems of 57 wolves from Galicia (from the provinces A Coruña n = 15, Lugo n = 21, Ourense n =15 and Pontevedra n = 6) using techniques of dissection and cup sedimentation. Collected worms were then identified under a light microscope according to their morphological features. Three species of nematodes were detected: Angiostrongylus vasorum (the “French-heartworm”), Crenosoma vulpis and Eucoleus aerophilus, the latter being of zoonotic interest. The prevalence was 24.5% (14/57; 95% CI 13.3–35.6%) overall, 19.3% for A. vasorum (11/57; 95% CI 8.8–29.2%), 7% for C. vulpis (4/57; 95% CI 0.4–13.6%) and 3.5% for E. aerophilus (2/57; CI −1.1–9.1%). A significant relationship (p = 0.002) was found between age and the presence of C. vulpis, which was only found in juvenile animals. Furthermore, a higher prevalence of A. vasorum and/or C. vulpis was observed in wolves with a lower body condition score (40% and 20%, respectively), though the difference was not significant (p = 0.221 and p = 0.444, respectively). Our findings indicate a high “French-heartworm” and lungworm burden in the wolf population of northern Spain, and they identify a need for studies designed to elucidate the epidemiological role played, Depto. de Sanidad Animal, Fac. de Veterinaria, TRUE, pub
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- 2022
10. Heavy metals and metalloid levels in the tissues of yellow-legged gulls (Larus michahellis) from Spain: sex, age, and geographical location differences
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Hernández Moreno, David [0000-0002-1189-9299], Vizuete, Jorge, Hernández Moreno, David, López-Beceiro, Ana, Fidalgo, Luis Eusebio, Soler, Francisco, Pérez-López, Marcos, Míguez-Santiyán, María Prado, Hernández Moreno, David [0000-0002-1189-9299], Vizuete, Jorge, Hernández Moreno, David, López-Beceiro, Ana, Fidalgo, Luis Eusebio, Soler, Francisco, Pérez-López, Marcos, and Míguez-Santiyán, María Prado
- Abstract
In the present study, mercury (Hg), cadmium (Cd), lead (Pb), selenium (Se), and arsenic (As) were measured in liver, kidney, and feathers of adult, juvenile, and chick seagulls (Larus michahellis) collected from the northwest of Spain. Age, sex, and the geographical location of samples were considered variables that can infuence metal bioaccumulation, for which concentrations were determined by means of ICP-MS. The mean concentrations (dry weight) found in seagulls were 7.01±0.37 mg Hg/kg, 22.82±2.83 mg Cd/kg, 7.36±1.36 mg Pb/kg, 18.64±0.63 mg Se/kg, and 10.64±0.59 mg As/kg. Regarding the diferent factors analyzed, Hg was the only metal showing sex-related diferences, being signifcantly higher (p<0.05) the concentrations found in feathers of males (1.26±0.12 mg/kg) than those in females (0.99±0.11 mg/kg). A highly signifcant (p<0.01) increase in levels of some metals was found in liver related to the increase of age: Hg (adults (A) 3.33±0.22 mg/kg vs chicks (C) 1.76±0.28 mg/kg), Cd (A 4.74±0.62 mg/kg vs C 1.79±0.2), Pb (A 0.65±0.12 mg/kg vs juveniles 0.4±0.11 mg/kg), and Se (A 7.56±0.43 mg/kg vs C 5.24±0.53 mg/kg). Positive correlations between Cd-Hg and Se–Hg were found in liver (p<0.001), kidney (p<0.001), and feathers (p<0.05 and p<0.001, respectively). The associa tions found may refect antagonistic interactions between Se and Cd on Hg toxicity. The results suggest that L. michahellis can reveal local contamination around the foraging and breeding sites and can be a very useful monitoring instrument for assessing heavy metal contamination and sentinel species of environmental health
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- 2022
11. Comparative Neuroanatomical Study of the Main Olfactory Bulb in Domestic and Wild Canids: Dog, Wolf and Red Fox
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Ortiz-Leal, Irene, primary, Torres, Mateo V., additional, López-Callejo, Linda Noa, additional, Fidalgo, Luis Eusebio, additional, López-Beceiro, Ana, additional, and Sanchez-Quinteiro, Pablo, additional
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- 2022
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12. Use of native chicken breeds ( Gallus gallus domesticus ) for the development of suitable methods of Cantabrian capercaillie ( Tetrao urogallus cantabricus ) semen cryopreservation
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O'Brien, Emma, primary, Castaño, Cristina, additional, Toledano‐Díaz, Adolfo, additional, Caamaño, José Néstor, additional, Hidalgo, Carlos, additional, Fidalgo, Luis Eusebio, additional, López‐Beceiro, Ana María, additional, Esteso, Milagros Cristina, additional, Balsera, Ramón, additional, García‐Casado, Pedro, additional, Łukaszewicz, Ewa, additional, and Santiago‐Moreno, Julián, additional
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- 2022
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13. High Prevalence and Diversity of Cephalosporin-Resistant Enterobacteriaceae Including Extraintestinal Pathogenic E. coli CC648 Lineage in Rural and Urban Dogs in Northwest Spain
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Abreu-Salinas, Fátima, primary, Díaz-Jiménez, Dafne, additional, García-Meniño, Isidro, additional, Lumbreras, Pilar, additional, López-Beceiro, Ana María, additional, Fidalgo, Luis Eusebio, additional, Rodicio, María Rosario, additional, Mora, Azucena, additional, and Fernández, Javier, additional
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- 2020
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14. Concentrations of chlorinated pollutants in adipose tissue of yellow-legged gulls (Larus michahellis) from Spain Role of gender and age
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Vizuete, Jorge [0009-0004-6859-1801], Vizuete, Jorge, Hernández Moreno, David, Fidalgo, Luis Eusebio, Bertini, Simonea, Andreini, R., Soler, Francisco, Míguez-Santiyán, María Prado, López-Beceiro, Ana, Pérez-López, Marcos, Vizuete, Jorge [0009-0004-6859-1801], Vizuete, Jorge, Hernández Moreno, David, Fidalgo, Luis Eusebio, Bertini, Simonea, Andreini, R., Soler, Francisco, Míguez-Santiyán, María Prado, López-Beceiro, Ana, and Pérez-López, Marcos
- Abstract
Concentrations of 7 different polychlorinated biphenyl (PCB) congeners, and eleven organochlorine pesticides (OCPs) and metabolites, including DDTs (dichlorodiphenyltrichloroethane), HCHs (hexachlorocyclohexane isomers), Endosulfan, Endosulfan sulfate, Endrin, Dieldrin and HCB (hexachlorobenzene), were determined in adipose tissue of 57 yellow-legged gulls collected from NW and N Spain. Furthermore, the possible differences due to two endogenous factors, age and gender, were determined. All the analyzed PCBs were detected in over 66% of the samples, with levels of 291.9 (PCB 180), 34.5 (PCB 118), 0.7 (PCB 28), 432.6 (PCB 153), 225.5 (PCB 138), 1.3 (PCB 101) and 0.4 (PCB 52) µg/kg of adipose tissue. With respect to the OCPs and metabolites, only 4,4'-DDE and HCB were detected in more than 50% of the samples, with means of 360.6 and 2.5 µg/kg of adipose tissue, respectively. From all the considered contaminants, only 4,4'-DDE levels presented significant differences depending on the gender, with females showing higher values than males (p < 0.01). Significant differences (p < 0.001) were also found related to age for the levels of PCBs 180, 138, 101, 28 and 153, as well as 4,4'-DDE, with adult levels being higher than those in young birds. The results of the present study constitute a baseline to better assess the environmental impacts of PCB and OCP contamination at other coastal sites for future biomonitoring studies, with particular emphasis on gender- and age-related differences.
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- 2018
15. Does gender influence the levels of heavy metals in liver of wild boar?
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Neila, C., Hernández Moreno, David, Fidalgo, Luis Eusebio, López-Beceiro, Ana, Soler, Francisco, Pérez-López, Marcos, Neila, C., Hernández Moreno, David, Fidalgo, Luis Eusebio, López-Beceiro, Ana, Soler, Francisco, and Pérez-López, Marcos
- Abstract
The aim of this study was to determine heavy metal reference levels for risk assessment studies. For this purpose, the levels of lead, cadmium, copper and zinc were determined in liver tissues of wild boars sampled in NW Spain. The mean values were 0.383, 0.326, 23.50 and 56.86 mg/kg dried weight, respectively. In general, the levels detected were similar to or lower than the levels reported in literature. This study not only provides a useful baseline for biomonitoring the levels of the analyzed contaminants in wildlife in NW Spain, it also helps to understand the effects of gender on the levels of these elements. Similar to studies performed in other geographical regions, no significant gender-related differences could be detected. Although differences were not significant, the levels of zinc, cadmium and lead were modestly higher in males (55.78, 0.346 and 0.424 mg/kg, respectively) compared to females (45.25, 0.305 and 0.341 mg/kg). Our results indicate that, although gender did not significantly affect heavy metal uptake and toxicokinetics of contaminants in wild boars, these effects could vary between species, populations, organs, and elements. It is therefore essential to investigate gender_x0002_related differences for each species.
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- 2017
16. A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle
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Verdes, José Manuel, primary, Márquez, Mercedes, additional, Calliari, Aldo, additional, Battes, Daniel, additional, Moraña, José Antonio, additional, Gimeno, Eduardo Juan, additional, Odriozola, Ernesto, additional, Giannitti, Federico, additional, Guerrero, Florentina, additional, Fidalgo, Luis Eusebio, additional, and Pumarola, Martí, additional
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- 2015
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17. Mercury and lead hepatic content in shag (Phalacrocorax aristotelis) and gannet (Morus bassanus) from the Galician coast (Spain)
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Soler, Francisco [0000-0002-4925-1092], Carbonell, G., Fernández-Torija, Carlos, López-Beceiro, Ana, Fidalgo, Luis Eusebio, Hernández Moreno, David, Soler, Francisco, Pérez-López, Marcos, Soler, Francisco [0000-0002-4925-1092], Carbonell, G., Fernández-Torija, Carlos, López-Beceiro, Ana, Fidalgo, Luis Eusebio, Hernández Moreno, David, Soler, Francisco, and Pérez-López, Marcos
- Abstract
At the present work, heavy metal (Hg and Pb) concentrations in hepatic tissue from two seabird species (shag, Phalacrocorax aristotelis, and gannet, Morus bassanus) have been analyzed, by using collected animals which died or were dying along the Galician coast (NW Spain). The considered animals were divided in two groups, considering if they had been affected or not by the oil spill caused by the "Prestige" in November 2002, and in order to determine if such spill had modified the heavy metal content of such seabird species. After sample wet digestion, heavy metal content was determined using anodic stripping voltammetry (Pb) and direct mercury analyzer (DMA) (Hg). When considering inter-specific differences for total Hg, hepatic concentrations from shag samples (mean 4.2-7.6 ppm) were higher than those from gannet (mean 1.6-1.8 ppm), with a maximum value reaching up to 20 ppm (wet weight), corresponding to an animal affected by the oil spill. With regards to Pb content, results were different, with slightly higher levels from gannet samples than those from shag, but never exceeding 0.01 ppm (wet weight). When considering both established groups (affected or not by the "Prestige" accident), it could be established that the oil spill did not affect on a statistically significant manner the levels of both metallic contaminants on the hepatic tissue of the considered animals.
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- 2007
18. LEAD TOXICITY IN CAPTIVE AND WILD MALLARDS (ANAS PLATYRHYNCHOS) IN SPAIN
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Rodríguez, Juan José, primary, Oliveira, Paula A., additional, Fidalgo, Luis Eusebio, additional, Ginja, Mário M. D., additional, Silvestre, António M., additional, Ordoñez, Cesar, additional, Serantes, Alicia Ester, additional, Gonzalo-Orden, José Manuel, additional, and Orden, María Asunción, additional
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- 2010
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19. Cerebellar Degeneration in Cattle Grazing Solanum Bonariense (“Naranjillo”) in Western Uruguay
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Verdes, José Manuel, primary, Moraña, Antonio, additional, Gutiérrez, Fernando, additional, Battes, Daniel, additional, Fidalgo, Luis Eusebio, additional, and Guerrero, Florentina, additional
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- 2006
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20. A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariensein cattle
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Verdes, José Manuel, Márquez, Mercedes, Calliari, Aldo, Battes, Daniel, Moraña, José Antonio, Gimeno, Eduardo Juan, Odriozola, Ernesto, Giannitti, Federico, Guerrero, Florentina, Fidalgo, Luis Eusebio, and Pumarola, Martí
- Abstract
Intoxication with Solanum bonariensein cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti–glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver–Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death.
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- 2015
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21. Cerebellar Degeneration in Cattle Grazing Solanum Bonariense(“Naranjillo”) in Western Uruguay
- Author
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Verdes, José Manuel, Moraña, Antonio, Gutiérrez, Fernando, Battes, Daniel, Fidalgo, Luis Eusebio, and Guerrero, Florentina
- Abstract
Cattle in western Uruguay that were eating Solanum bonariensedeveloped periodic episodes of ataxia, hypermetria, hyperesthesia, head and thoracic limb extension, opisthotonus, nystagmus, and falling to the side or backward. Similar clinical signs were experimentally reproduced in cattle by administration of S. bonariensevia rumen cannula at a dose of 1,024 g/kg body mass. No significant gross lesions were observed in field cases or experimentally induced cases. Spontaneous and induced histologic lesions were similar and included vacuolation, degeneration, and loss of Purkinje cells. Axonal spheroids, microcavitations, and other changes of wallerian-type degeneration in cerebellar white matter were also observed. Ultrastructural changes included increased number of electron-dense residual storage bodies in membrane-bound vesicles in affected Purkinje cells, and similar vesicles and mitochondria in axonal spheroids. No histologic lesions were detected in the other examined tissues. The Purkinje-cell swelling and vacuolation with subsequent cerebellar degeneration are suggestive of Purkinje-cell specific toxin that produces abnormal lysosome function and cell specific axonal transport. This is the first report of S. bonariensetoxicity.
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- 2006
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22. Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation
- Author
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Emma O'Brien, Cristina Castaño, Adolfo Toledano‐Díaz, José Néstor Caamaño, Carlos Hidalgo, Luis Eusebio Fidalgo, Ana María López‐Beceiro, Milagros Cristina Esteso, Ramón Balsera, Pedro García‐Casado, Ewa Łukaszewicz, Julián Santiago‐Moreno, Fundación Biodiversidad, Ministerio para la Transición Ecológica y el Reto Demográfico (España), Castaño, Cristina, Toledano-Díaz, Adolfo, Caamaño, José Néstor, Fidalgo, Luis Eusebio, Esteso, Milagros Cristina, García-Casado, Pedro, Łukaszewicz, Ewa, Santiago-Moreno, Julián, Castaño, Cristina [0000-0003-1134-1436], Toledano-Díaz, Adolfo [0000-0001-6679-485X], Caamaño, José Néstor [0000-0001-6449-5784], Fidalgo, Luis Eusebio [0000-0001-9184-0942], Esteso, Milagros Cristina [0000-0002-8963-5736], García-Casado, Pedro [0000-0002-1242-1035], Łukaszewicz, Ewa [0000-0002-9505-0056], and Santiago-Moreno, Julián [0000-0001-5551-8120]
- Subjects
Cryopreservation ,Glycerol ,Male ,General Veterinary ,Pilot Projects ,Cryoprotectant ,Semen Analysis ,Plant Breeding ,Cryoprotective Agents ,Extender ,Sperm freezing ,Seeds ,Sperm Motility ,Animals ,Capercaillie ,Chickens ,Semen Preservation - Abstract
8 Pág., The Cantabrian capercaillie (Tetrao urogallus cantabricus) is critically endangered. This subspecies has the lowest genetic variability and it is in regression. It belongs to Phasianidae family; therefore, the domestic chicken (Gallus gallus domesticus) could be a good model for developing reproductive technologies for use in capercaillie populations with low availability of animals., Fundación Biodiversidad-Ministerio para la Transición Ecológica y el Reto Demográfico (Ref-DOC-2017458); Zoitechlab S.L.-INIA, Grant/Award Number: CON18-141
- Published
- 2022
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