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More than 50% of solar-mass stars form in multiple systems. It is therefore crucial to investigate how multiplicity affects the star and planet formation processes at the protostellar stage. We report continuum and C$^{18}$O (2-1) observations of the VLA 1623-2417 protostellar system at 50 au angular resolution as part of the ALMA Large Program FAUST. The 1.3 mm continuum probes the disks of VLA 1623A, B, and W, and the circumbinary disk of the A1+A2 binary. The C$^{18}$O emission reveals, for the first time, the gas in the disk-envelope of VLA 1623W. We estimate the dynamical mass of VLA 1623W, $M_{\rm dyn}=0.45\pm0.08$ M$_{\odot}$, and the mass of its disk, $M_{\rm disk}\sim6\times10^{-3}$ M$_{\odot}$. C$^{18}$O also reveals streamers that extend up to 1000 au, spatially and kinematically connecting the envelope and outflow cavities of the A1+A2+B system with the disk of VLA 1623W. The presence of the streamers, as well as the spatial ($\sim$1300 au) and velocity ($\sim$2.2 km/s) offset of VLA 1623W suggest that either sources W and A+B formed in different cores, interacting between them, or that source W has been ejected from the VLA 1623 multiple system during its formation. In the latter case, the streamers may funnel material from the envelope and cavities of VLA 1623AB onto VLA 1623W, thus concurring to set its final mass and chemical content., Comment: 10 pages, 5 figures, accepted by MNRAS

4 Pág., Cetacean morbillivirus (CeMV) causes illness and death in cetaceans worldwide; the CeMV strains circulating in the Southern Hemisphere are poorly known. We detected a pilot whale CeMV strain in 3 short-finned pilot whales (Globicephala macrorhynchus) stranded in Brazil during July-October 2020. Our results confirm this virus circulates in this species., The Coordination for the Improvement of Higher Education Personnel (CAPES), National Council for Technological and Scientific Development (CNPq), and São Paulo Research Foundation (FAPESP) provided financial support. S.C.S. and A.C.E. received PhD fellowships by FAPESP (process nos. 2020/12434-9 and 2016/20956-0). L.B.K. received financial support from FAPESP (no. 2020/12434-9). J.L.C.-D., L.B.K. and M.J.C. are recipients of research productivity fellowships from CNPq (nos.304999-18, 315619/2021-0, and 313577/2020-0, respectively),C.S. is a recipient of a Juan de la Cierva incorporación fellowship (no. IJC2020-046019-I) and received a postdoctoral grant by FAPESP (no. 2018/25069-7)

One possible consequence of dentoalveolar trauma is the development of external inflammatory root resorption (EIRR), which represents an anatomic and microbiologic challenge for clinicians. This case report describes different strategies implemented for successful endodontic management of teeth with multiple EIRR lesions, highlighting the orthograde root canal filling using a tricalcium silicate-based material (Biodentine, Septodont, Saint-Maur-des-Fossés, France). A 17-year-old female patient presented with severe pain in the anterior maxillary teeth and a history of trauma. Two- and three-dimensional radiographic exams confirmed EIRR in three teeth, with a total of 11 EIRR lesions, three exhibiting communication with the root canal. Therefore, chemo-mechanical preparation complemented by ultrasonic activation of irrigants and some changes of intra-canal dressing with calcium hydroxide were performed to reduce the microbiologic load of the affected teeth as much as possible. Then, the canals were entirely filled with Biodentine to interrupt the root resorption process and strengthen the remaining root structure. A 60-month follow-up showed the disappearance of bone rarefactions and the complete repair of the 11 EIRR lesions. The favorable long-term response indicates the feasibility of using tricalcium silicate-based putty as part of orthograde endodontic treatment of teeth with EIRR and root perforations.

Purpose Primary Health Care (PHC) is the coordinator of health care in Brazil and needs to be strengthened in the diagnostic field to increase health care quality. Aiming to improve the diagnostic tools currently available in PHC, this work describes the process of development and validation of two point-of-care biomedical devices for screening patients with syphilis or different kinds of cancer. Methods The development of these devices followed nine stages of action based on the requirements established by the Ministry of Health. During development, both systems followed the stages of circuit planning, software simulation to verify the components used, cost assessment for the acquisition of features, simulation in contact matrix, development of the embedded system, and planning of the printed circuit board and storage box. Results Both devices underwent preliminary functionality tests to assess their quality. The performance tests applied on the device to diagnose syphilis performed 8,733,194 requests, with a flow of 2426 requests/second, reaching the desired parameters of robustness, integrity, durability, and stability. In addition, functioning tests on the cancer-screening device indicated the ability to detect standard fluorescence in a minimal (150 uL) sample volume. Conclusions Together, the methodology used for developing the devices resulted in promising equipment to improve the diagnosis and meet the requirements for executing technologies for testing and triaging patients in PHC.

Radiography is a common imaging method for non-destructive object inspection. Processing radiographs to detect defects is, however, challenging, especially if the defect type and location are unknown. In other fields, autoencoders (AE) have been largely studied for anomaly detection. While their performance for defect detection has shown promise, many solutions are not demonstrated to be defect-independent. In this work, we introduce a radiography-based anomaly detection method that first computes the AE prediction error from an input radiograph to enhance the detectability of defects. Subsequently, the probability of the imaged object being defective is predicted by a convolutional classification network. Results on simulated data demonstrate that this method succeeds in distinguishing between defective and nondefective objects, with accuracy (which calculates how often the predictions are equal to the labels) >94% and precision (which is the ratio between the true positives and the total predicted positive) >90%, even for defects unseen during the training stage. Our classification network showed high robustness to noise (with signal-to-noise-ratio (SNR) of 20 dB), and accuracy on noisy data >90% and precision >86%. Since our network workflow does not require image registration for defect detection, the proposed solution is therefore independent of the orientation and position of the object during the scan.

Objective: To assess the centralization and dentin thickness of mesial root canals by microcomputed tomography (micro-CT). Methods: Ninety-nine mandibular molars Vertucci’s type IV mesial canals were scanned by micro-CT. The mesiodistal deviation; and the centroid were calculated for mesiobuccal (MB) and mesiolingual (ML) canals (apical 4 mm and full canal). Results: The centroid was deviated in MB and ML canals in full canal (MB=0.83 mm, 0.02-2.30 mm, and ML=0.83 mm, 0.05-3.99 mm), and in 4mm (MB=0.18mm,0.01-1.01 mm and ML=0.21 mm, 0.01-1.01 mm). In the full canal, both MV (69%) e ML (57%) deviated to mesial (M). In 4 mm, the deviation was M in 51% for MB canals and 52% of ML to distal (D). The dentin thickness was similar for MB and ML canals and their walls. Distal wall was lower for MB canal (0.07 mm) and M wall the higher in the MB canal (2.46 mm). Conclusions: There is no centrality of mesial canals in mandibular first molars. The M deviation was frequent in the full canal. Dentin thickness were similar between mesial canals.

Objective The effects of brushing on shaping with different instruments was assessed in oval canals. Design Mandibular incisors were assigned to 6 groups (n = 12/group) according to the system, each one with or without brushing: Reciproc Blue, VDW.Rotate, and Race EVO. Micro-computed tomography was performed before and after preparation. Results Brushing strokes caused no increase in canal volume, surface area, and structure model index independently of the system (p > 0.05), except RaCe in full canal surface area (p 0.05), except for Reciproc in apical canal (p

Birds, cartilaginous and teleost fish, reptiles, and some amphibians have intrascleral cartilage and/or bone; however, these are rarely reported in therian mammals. This study aimed to investigate and characterize a nonpathological formation of cartilage in the posterior sclera of sheep macroscopically, histologically, and by immunohistochemical exam (IHC). Ninety eyes from 45 domestic sheep were collected, underwent gross examination, fixed in formalin, and embedded in paraffin for the microscopical assessment. Sections with histological shreds of cartilage were selected to perform IHC to confirm the presence of cartilage. Intrascleral cartilage was detected in 60 eyeballs (66.66%) from 37 sheep (82.22%). A slight whitish thickening was grossly seen in the posterior sclera. The histologic exam revealed a few scattered, isolated chondrocytes to larger aggregates of cartilaginous islands in the posterior sclera. Eighteen (30%) of 60 eyeballs revealed marked anti-collagen type II immunolabeling. The development of cartilaginous structures in the eyes is considered rare in mammalian animals. The high occurrence of intrascleral cartilage in the examined sheep eyes suggests that this finding corresponds to an anatomical component of sheep sclera, despite the age, breed, or body condition. RESUMO: Aves, peixes cartilaginosos e teleósteos e répteis, bem como alguns anfíbios possuem cartilagem e/ou osso intraescleral, contudo, isso é raramente descrito em marsupiais. O objetivo deste trabalho foi investigar e caracterizar a formação não patológica de cartilagem hialina na esclera posterior de ovinos, por exame macroscópico, histológico e imuno-histoquímico. Olhos de 45 ovinos foram coletados, submetidos a exame macroscópico, fixados em formalina a 10% tamponada e incluídos em parafina para o exame histológico. Amostras com evidência de formação cartilaginosa ao exame histológico foram selecionadas para a realização da técnica de imuno-histoquímica para confirmação. Cartilagem intraescleral foi detectada em 60 amostras oculares (66,66%) de 37 ovinos (82,22%), caracterizada na avaliação macroscópica como discretos espessamentos esbranquiçados na esclera posterior. O exame histológico revelou formações que variaram de condrócitos isolados e dispersos a grandes agregados, formando ilhas cartilaginosas na esclera. Dezoito (30%) de 60 bulbos oculares mostraram imunomarcação anti-colágeno tipo II. O desenvolvimento de estruturas cartilaginosas nos bulbos oculares é considerado raro em mamíferos. A identificação de cartilagem intraescleral em 66% dos olhos ovinos avaliados no estudo indica que a formação de cartilagem corresponde a um componente anatômico normal na esclera de ovinos, independente de idade, raça ou condição corporal.

ObjectiveTo evaluate the effectiveness of two technology-enhanced interventions for diabetes prevention among adults at risk for developing diabetes in a primary care setting.MethodsThe DiaBEAT-it study employed a hybrid 2-group preference (Choice) and 3-group randomized controlled (RCT) design. This paper presents weight related primary outcomes of the RCT arm. Patients from Southwest Virginia were identified through the Carilion Clinic electronic health records. Eligible participants (18 and older, BMI ≥ 25, no Type 2 Diabetes) were randomized to either Choice (n = 264) or RCT (n = 334). RCT individuals were further randomized to one of three groups: (1) a 2-h small group class to help patients develop a personal action plan to prevent diabetes (SC, n = 117); (2) a 2-h small group class plus automated telephone calls using an interactive voice response system (IVR) to help participants initiate weight loss through a healthful diet and regular physical activity (Class/IVR, n = 110); or (3) a DVD with same content as the class plus the same IVR calls over a period of 12 months (DVD/IVR, n = 107).ResultsOf the 334 participants that were randomized, 232 (69%) had study measured weights at 6 months, 221 (66%) at 12 months, and 208 (62%) at 18 months. Class/IVR participants were less likely to complete weight measures than SC or DVD/IVR. Intention to treat analyses, controlling for gender, race, age and baseline BMI, showed that DVD/IVR and Class/IVR led to reductions in BMI at 6 (DVD/IVR −0.94, p < 0.001; Class/IVR −0.70, p < 0.01), 12 (DVD/IVR −0.88, p < 0.001; Class/IVR-0.82, p < 0.001) and 18 (DVD/IVR −0.78, p < 0.001; Class/IVR −0.58, p < 0.01) months. All three groups showed a significant number of participants losing at least 5% of their body weight at 12 months (DVD/IVR 26.87%; Class/IVR 21.62%; SC 16.85%). When comparing groups, DVD/IVR were significantly more likely to decrease BMI at 6 months (p < 0.05) and maintain the reduction at 18 months (p < 0.05) when compared to SC. There were no differences between the other groups.ConclusionsThe DiaBEAT-it interventions show promise in responding to the need for scalable, effective methods to manage obesity and prevent diabetes in primary care settings that do not over burden primary care clinics and providers.Registrationhttps://clinicaltrials.gov/ct2/show/NCT02162901, identifier: NCT02162901.

In this paper, the Hamiltonian structure of the bosonized chiral Schwinger model (BCSM) is analyzed. From the consistency condition of the constraints obtained from the Dirac method, we can observe that this model presents, for certain values of the $\alpha$ parameter, two second-class constraints, which means that this system does not possess gauge invariance. However, we know that it is possible to disclose gauge symmetries in such a system by converting the original second-class system into a first-class one. This procedure can be done through the gauge unfixing (GU) formalism by acting with a projection operator directly on the original second-class Hamiltonian, without adding any extra degrees of freedom in the phase space. One of the constraints becomes the gauge symmetry generator of the theory and the other one is disregarded. At the end, we have a first-class Hamiltonian satisfying a first-class algebra. Here, our goal is to apply a new scheme of embedding second-class constrained systems based on the GU formalism, named improved GU formalism, in the BCSM. The original second-class variables are directly converted into gauge invariant variables, called GU variables. We have verified that the Poisson brackets involving the GU variables are equal to the Dirac brackets between the original second-class variables. Finally, we have found that our improved GU variables coincide with those obtained from an improved BFT method after a particular choice for the Wess-Zumino terms., Comment: 13 pages

In recent years, the use of Public Service Obligations (PSOs) in air transport policy has substantially increased. In remote regions, where air transport services are not profitable, the application of this subsidy program is crucial for the economic and social development of these regions. However, there is a great diversity in the provision of PSO services among different regions in Europe. Therefore, it is essential to assess the performances of the air carriers operating in different PSO routes. The present study uses Data Envelopment Analysis (DEA) to assess the efficiency of PSO routes within the European Union and compares them with the performances of the operating airlines, both when operating under this subsidy program and when operating their regular flights. First, DEA was applied to the annual data of several European airlines, and then applied to the data of their PSO routes, so that a comparison can be made between the performance obtained on regular flights and flights under the PSO regime. The results identify which airlines had the best and worst performances, as well as the routes and respective regions of Europe where the airlines tend to score higher and lower in terms of efficiency. Results suggest that the Nordic European countries and the United Kingdom were the regions where air carriers obtained the lowest results, and thus, this empirical evidence should support the public authorities to consider revisiting their PSO contracts, in order to improve their operation. info:eu-repo/semantics/acceptedVersion

Made available in DSpace on 2022-05-01T08:45:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 We investigate several notions related to pseudotrajectories, including chain recurrence and shadowing properties, for a special class of diffeomorphisms on euclidean spheres, known as spherical linear transformations, and for bounded linear operators on Banach spaces. Our main results are complete characterizations of chain recurrence for spherical linear transformations on euclidean spheres and for weighted shifts on the classical Banach sequence spaces c and ℓp. Another main result is a characterization of hyperbolicity for invertible operators on Banach spaces by means of average expansivity and the average shadowing property. Instituto Federal de Educação Ciência e Tecnologia de São Paulo Campus Presidente Epitácio, Rua José Ramos Junior, 27-50 Departamento de Matemática Aplicada Instituto de Matemática Universidade Federal do Rio de Janeiro, Caixa Postal 68530 Departamento de Matemática Universidade Estadual Paulista, Rua Cristóvão Colombo, 2265, Jardim Nazareth Departamento de Matemática Universidade Estadual Paulista, Rua Cristóvão Colombo, 2265, Jardim Nazareth

Introduction The WHO Prison Health Framework was developed to assess prison health system performance and support Member States (MS) in improving their prison health systems. Moreover, it shall enhance MS capacity to evaluate: the impact of changes in governance models, progress in service provision and improvements of the health status of people in prison (PiP). Methods The framework informed the 2021 data collection round of the Health In Prisons European Database Survey. Invitations were sent to all 53 MS of the WHO European Region. Those MS nominating a focal point and providing valid answers were included in the analysis. Results Answers were obtained from 36 MS, representing a total of 613,497 PiP. Access to immunization was very good across all MS, with the highest for COVID-19 (90% of MS provide it in all prisons). Vaccination against HBV was only available in all prisons of 25 MS. Access in all prisons to HIV post and PrEP were reported, respectively, by 78% and 58% of MS. Screening for diseases at entrance was common for HIV, HCV and HBV. In all prisons of 35 MS soap was provided for free, while needles & syringes and lubricants were only provided free of charge, respectively, in 3 and 4 MS. 5 MS did not have therapeutic spaces to tackle drug problems in any prison, in 73% of those having, accessibility was restricted to some prisons. HIV prevalence ranged from 0-16% and treatment was accessible to 55-100% of those diagnosed. Prevalence of HCV ranged from 0-34%, with access to treatment ranging from 0-91%. The most common format of health records in European prisons was paper based (44%). Conclusions Prison-based data collection systems resulted in limited capacity for extraction so that some countries were unable to provide any data on disease prevalence or treatments offered. Given the scarcity of data on this topic obtained from real-world and not from ad-hoc studies, this snapshot provides an important contribution to public health.

Given the potential of acid-activated clays in adsorptive and catalytic processes, several studies focusing on acid matrices have grown over the years, making it possible to study vermiculite, a mineral composed of a type 2:1 unit cell with tetrahedral and octahedral sheets. Therefore, this work aimed to study vermiculite treated with acids for the production of matrices. In this work, the treatment of natural vermiculite-hydrobiotite, with nitric acid in concentrations of 0.25, 0.50, 0.75, and 1 mol.L-1, followed by the dealumination process with sulfuric acid 25% v/v, aiming the production of acid matrices for applications as support for biocatalysts, chemical adsorbents, among others. The produced materials were characterized using different techniques, including X-ray fluorescence spectroscopy, X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and isotherm adsorption using the BET method. The results showed that after acid leaching the vermiculite showed a high SiO2 content, high Brønsted and Lewis surface acidity, good adsorption/desorption performance, and significant growth of the specific surface area, qualifying the vermiculite as a promising material for future applications in catalytic and biocatalytic processes. The acid concentration had a considerable influence on the compositional and textural modification of vermiculite-hydrobiotite.

The 3D musculoskeletal motion of animals is of interest for various biological studies and can be derived from X-ray fluoroscopy acquisitions by means of image matching or manual landmark annotation and mapping. While the image matching method requires a robust similarity measure (intensity-based) or an expensive computation (tomographic reconstruction-based), the manual annotation method depends on the experience of operators. In this paper, we tackle these challenges by a strategic approach that consists of two building blocks: an automated 3D landmark extraction technique and a deep neural network for 2D landmarks detection. For 3D landmark extraction, we propose a technique based on the shortest voxel coordinate variance to extract the 3D landmarks from the 3D tomographic reconstruction of an object. For 2D landmark detection, we propose a customized ResNet18-based neural network, BoneNet, to automatically detect geometrical landmarks on X-ray fluoroscopy images. With a deeper network architecture in comparison to the original ResNet18 model, BoneNet can extract and propagate feature vectors for accurate 2D landmark inference. The 3D poses of the animal are then reconstructed by aligning the extracted 2D landmarks from X-ray radiographs and the corresponding 3D landmarks in a 3D object reference model. Our proposed method is validated on X-ray images, simulated from a real piglet hindlimb 3D computed tomography scan and does not require manual annotation of landmark positions. The simulation results show that BoneNet is able to accurately detect the 2D landmarks in simulated, noisy 2D X-ray images, resulting in promising rigid and articulated parameter estimations.

Evaluating the epigenetic landscape in the stem cell compartment at the single-cell level is essential to assess the cells’ heterogeneity and predict their fate. Here, using a genome-wide transcriptomics approach in vivo, we evaluated the allelic expression imbalance in the progeny of single hematopoietic cells (HSCs) as a read-out of epigenetic marking. After 4 months of extensive proliferation and differentiation, we found that X-chromosome inactivation (XCI) is tightly maintained in all single-HSC derived hematopoietic cells. In contrast, the vast majority of the autosomal genes did not show clonal patterns of random monoallelic expression (RME). However, a persistent allele-specific autosomal transcription in HSCs and their progeny was found in a rare number of cases, none of which has been previously reported. These data show that: 1) XCI and RME in the autosomal chromosomes are driven by different mechanisms; 2) the previously reported high frequency of genes under RME in clones expanded in vitro (up to 15%) is not found in clones undergoing multiple differentiation steps in vivo; 3) prior to differentiation, HSCs have stable patterns of autosomal RME. We propose that most RME patterns in autosomal chromosomes are erased and established de novo during cell lineage differentiation.

© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attri- bution 4.0 International License, which permits use, sharing, adapta- tion, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/., Purpose: There is limited data concerning neuroimaging findings and longitudinal evaluation of familial cerebral cavernous malformations (FCCM) in children. Our aim was to study the natural history of pediatric FCCM, with an emphasis on symptomatic hemorrhagic events and associated clinical and imaging risk factors. Methods: We retrospectively reviewed all children diagnosed with FCCM in four tertiary pediatric hospitals between January 2010 and March 2022. Subjects with first available brain MRI and [Formula: see text] 3 months of clinical follow-up were included. Neuroimaging studies were reviewed, and clinical data collected. Annual symptomatic hemorrhage risk rates and cumulative risks were calculated using survival analysis and predictors of symptomatic hemorrhagic identified using regression analysis. Results: Forty-one children (53.7% males) were included, of whom 15 (36.3%) presenting with symptomatic hemorrhage. Seven symptomatic hemorrhages occurred during 140.5 person-years of follow-up, yielding a 5-year annual hemorrhage rate of 5.0% per person-year. The 1-, 2-, and 5-year cumulative risks of symptomatic hemorrhage were 7.3%, 14.6%, and 17.1%, respectively. The latter was higher in children with prior symptomatic hemorrhage (33.3%), CCM2 genotype (33.3%), and positive family history (20.7%). Number of brainstem (adjusted hazard ratio [HR] = 1.37, P = 0.005) and posterior fossa (adjusted HR = 1.64, P = 0.004) CCM at first brain MRI were significant independent predictors of prospective symptomatic hemorrhage. Conclusion: The 5-year annual and cumulative symptomatic hemorrhagic risk in our pediatric FCCM cohort equals the overall risk described in children and adults with all types of CCM. Imaging features at first brain MRI may help to predict potential symptomatic hemorrhage at 5-year follow-up.

The invasion of Ukraine by Russia in February 2022 has resulted in destruction of healthcare infrastructure and triggered the largest wave of internally displaced populations and refugees since World War Two. Conflicts in transitioned countries such as Ukraine create new non-communicable disease (NCD) challenges, especially for cancer care for refugees and humanitarian assistance in host countries. In the early days, rapid attempts were made to model possible impacts. By evaluating open source intelligence used in the first three months of the conflict through snowball search methods, we aimed to address: (i) burden of cancer in Ukrainian population, specifically considering translating to the refugees population, and its cancer care capacity; ii) baseline capacity/strengths of cancer systems in initial host countries. Moreover, using a baseline scenario based on crude cancer incidence in Ukraine, and considering data from UNHCR, we estimated how cancer cases would be distributed across host countries. Finally, a surveillance assessment instrument was created, intersecting health system's capacity and influx of internally displaced populations and refugees. The total new cancer patients per month in pre-conflict Ukraine was estimated as 13,106, of which < 1 % are paediatric cases. The estimated cancer cases in the refugee population (combining prevalent and incident), assuming 7.5 million refugees by July 2022 and a female:male ratio of 9:1, was 33,121 individuals (Poland: 19284; Hungary: 3484; Moldova: 2651; Slovakia: 2421; Romania: 5281). According to our assessments, Poland is the only neighbouring country classified as green/yellow for cancer capacity, i.e. sufficient ablility to absorb additional burden into national health system; Slovakia we graded as yellow, Hungary and Romania as yellow/red and Moldova as red.

C. Codella et al., The ALMA (Atacama Large Millimeter Array) interferometer, with its unprecedented combination of high sensitivity and high angular resolution, allows for (sub-)mm wavelength mapping of protostellar systems at Solar system scales. Astrochemistry has benefitted from imaging interstellar complex organic molecules in these jet–disc systems. Here, we report the first detection of methanol (CH3OH) and methyl formate (HCOOCH3) emission towards the triple protostellar system VLA1623−2417 A1+A2+B, obtained in the context of the ALMA Large Programme FAUST (Fifty AU STudy of the chemistry in the disc/envelope system of solar-like protostars). Compact methanol emission is detected in lines from Eu = 45 K up to 61 K and 537 K towards components A1 and B, respectively. Large velocity gradient analysis of the CH3OH lines towards VLA1623−2417 B indicates a size of 0.11–0.34 arcsec (14–45 au), a column density NCH3OH = 1016–1017 cm−2, kinetic temperature ≥ 170 K, and volume density ≥ 108 cm−3. A local thermodynamic equilibrium approach is used for VLA1623−2417 A1, given the limited Eu range, and yields Trot ≤ 135 K. The methanol emission around both VLA1623−2417 A1 and B shows velocity gradients along the main axis of each disc. Although the axial geometry of the two discs is similar, the observed velocity gradients are reversed. The CH3OH spectra from B show two broad (4–5 km s−1) peaks, which are red- and blueshifted by ∼ 6–7 km s−1 from the systemic velocity. Assuming a chemically enriched ring within the accretion disc, close to the centrifugal barrier, its radius is calculated to be 33 au. The methanol spectra towards A1 are somewhat narrower (∼ 4 km s−1), implying a radius of 12–24 au., This project has received funding from: (1) the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme, for the Project ‘The Dawn of Organic Chemistry’ (DOC), grant agreement no. 741002; (2) the PRIN-INAF 2016 The Cradle of Life – GENESIS-SKA (General Conditions in Early Planetary Systems for the rise of life with SKA); (3) a Grant-in-Aid from Japan Society for the Promotion of Sci- ence (KAKENHI: Nos. 18H05222, 20H05844, 20H05845); (4) the Spanish FEDER under project number ESP2017-86582-C4-1-R; (5) DGAPA, UNAM grants IN112417 and IN112820, and CONACyT, Mexico; (6) ANR of France under contract number ANR-16-CE31- 0013; (7) the French National Research Agency in the framework of the Investissements d’Avenir programme (ANR-15-IDEX-02), through the funding of the ‘Origin of Life’ project of the Univ. Grenoble-Alpes, (8) the European Union’s Horizon 2020 research and innovation programmes under projects ‘Astro-Chemistry Ori- gins’ (ACO), Grant No. 811312; (9) the National Research Council Canada and an NSERC Dico v ery Grant to DJ. This paper makes use of the following ALMA data: ADS/JAO.ALMA#2018.1.01205.L. ALMA is a partnership of ESO (representing its member states), NSF (USA) and NINS (Japan), together with NRC (Canada), MOST and ASIAA (Taiwan), and KASI (Republic of Korea), in cooperation with the Republic of Chile. The Joint ALMA Obser- vatory is operated by ESO, A UI/NRA O, and NAOJ. The National Radio Astronomy Observatory is a facility of the National Science Foundation operated under cooperative agreement by Associated Universities, Inc.

Funder: Agua Salud Project, Funder: U.S. Department of Energy; Id: http://dx.doi.org/10.13039/100000015, Funder: CAPES; Id: http://dx.doi.org/10.13039/501100002322, Data capturing multiple axes of tree size and shape, such as a tree's stem diameter, height and crown size, underpin a wide range of ecological research-from developing and testing theory on forest structure and dynamics, to estimating forest carbon stocks and their uncertainties, and integrating remote sensing imagery into forest monitoring programmes. However, these data can be surprisingly hard to come by, particularly for certain regions of the world and for specific taxonomic groups, posing a real barrier to progress in these fields. To overcome this challenge, we developed the Tallo database, a collection of 498,838 georeferenced and taxonomically standardized records of individual trees for which stem diameter, height and/or crown radius have been measured. These data were collected at 61,856 globally distributed sites, spanning all major forested and non-forested biomes. The majority of trees in the database are identified to species (88%), and collectively Tallo includes data for 5163 species distributed across 1453 genera and 187 plant families. The database is publicly archived under a CC-BY 4.0 licence and can be access from: https://doi.org/10.5281/zenodo.6637599. To demonstrate its value, here we present three case studies that highlight how the Tallo database can be used to address a range of theoretical and applied questions in ecology-from testing the predictions of metabolic scaling theory, to exploring the limits of tree allometric plasticity along environmental gradients and modelling global variation in maximum attainable tree height. In doing so, we provide a key resource for field ecologists, remote sensing researchers and the modelling community working together to better understand the role that trees play in regulating the terrestrial carbon cycle.

Made available in DSpace on 2021-06-25T10:56:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Animal models are widely used for studying diabetes in translational research. However, methods for induction of diabetes are conflicting with regards to their efficacy, reproducibility and cost. A comparison of outcomes between the diabetic models is still unknown, especially full-term pregnancy.To understand the comparison, we analyzed the streptozotocin (STZ)-induced diabetes at three life-different moments during the neonatal period in Sprague–Dawley female rats: at the first (D1), second (D2) and fifth (D5) day of postnatal life. At adulthood (90 days; D90), the animals were submitted to an oral glucose tolerance test (OGTT) for diabetic status confirmation. The diabetic and control rats were mated and sacrificed at full-term pregnancy for different analyses. Group D1 presented a higher mortality percentage after STZ administration than groups D2 and D5. All diabetic groups presented higher blood glucose levels as compared to those of the control group, while group D5 had higher levels of glycemia compared with other groups during OGTT. The diabetic groups showed impaired reproductive outcomes compared with the control group. Group D1 had lower percentages of mated rats and D5 showed a lower percentage of a full-term pregnancy. Besides that, these two groups also showed the highest percentages of inadequate fetal weight. In summary, although all groups fulfill the diagnosis criteria for diabetes in adult life, in our investigation diabetes induced on D5 presents lower costs and higher efficacy and reproducibility for studies involving diabetes-complicated pregnancy. Laboratory of Experimental Research on Gynecology and Obstetrics São Paulo State University (UNESP) DNA Repair Laboratory University of São Paulo (USP) Joslin Diabetes Center Harvard Medical School Department of Physiology Charles University Laboratory of System Physiology and Reproductive Toxicology Federal University of Mato Grosso (UFMT) Laboratory of Experimental Research on Gynecology and Obstetrics São Paulo State University (UNESP)

Made available in DSpace on 2021-06-25T11:51:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Instituto Federal Goiano (IFGoiano) Universidade Federal do Rio de Janeiro (UFRJ) -Brazil Background: Food proteins have benefits for human health, which justifies their production and use. In this context, the use of seeds and byproducts that would be otherwise discarded is highlighted in the present work, to produce protein extracts and hydrolyzed proteins, generating opportunities to reduce environmental impacts. Objective: This work aimed to use different extraction methods to obtain protein extracts from seeds (corn, sorghum, and sunflower) and sunflower byproduct to determine their antioxidant activity, and apply different proteolytic enzymes in the hydrolysis of sunflower byproduct. Methods: The seeds of corn, sorghum, and sunflower, and sunflower byproduct were ground to produce flour and the protein extracts were prepared using five different methods. The bioactivity of fractions was analyzed by different methods (ABTS, DPPH, and FRAP) to evaluate the antioxidant activity. Results: The most effective methods, which resulted in higher protein extraction and antioxidant activity, were those in which NH4HCO3 (5 mM, pH 8.0) and H2O/C2H6O (2:3) were used. The highest protein contents were 797.9, 303.8, and 11296.5 mu g/g, and the highest antioxidant activity was 34417.5, 9732.6, and 47473.1 mu g TE/g from Soxhlet and Bligh and Dyer defatted extractions for sunflower seed, and sunflower byproduct, respectively. Regarding enzymatic hydrolysis, sunflower byproduct was the substrate that presented the highest degree of hydrolysis (11.06%) when Neutrase (R) enzyme was used. Enzymatic hydrolysis increased antioxidant activity in the hydrolyzed proteins, approximately by 20.0%, using Neutrase (R) Neutrase and 22.3% using Flavourzyme (R) Flavourzyme treatments. Conclusion: The protein extracts and the hydrolyzed proteins exhibited high antioxidant activity, demonstrating great potential for use as natural antioxidants in food systems. Goiano Fed Inst Educ Sci & Technol, Campus Rio Verde,Rodovia Sul Goiana,Km 01, BR-75901970 Rio Verde, Go, Brazil Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP, Brazil Fed Univ Rio de Janeiro UFRJ, Sch Chem, Dept Biochem Engn, Athos da Silveira Ramos 149, BR-21941909 Rio De Janeiro, Brazil Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP, Brazil CAPES: 001 Instituto Federal Goiano (IFGoiano): 23218.001954/2019-16