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Made available in DSpace on 2022-04-29T08:29:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Summary In vitro production of embryos has gained prominence as a tool for use in wildlife conservation programmes in situ and ex situ. However, the development of this technique depends on steps that include ovarian stimulation, collection and oocyte maturation. The purpose of this study was to assess the feasibility of an ovarian stimulation protocol for follicular aspiration, the efficiency of videolaparoscopy for follicular aspiration and test a medium for in vitro oocyte maturation for the species Mazama gouazoubira. Five females were submitted to repeated ovarian stimulation (hormone protocol using controlled internal drug release), and estradiol benzoate on D0 and eight injections of follicle-stimulating hormone, once every 12 h, from D4 onwards at 30-day intervals. Fourteen surgical procedures were performed in superstimulated females, resulting in the collection of 94 oocytes and an average of 17.1 ± 9.1 follicles observed, 13.5 ± 6.6 follicles aspirated and 7.2 ± 3.7 oocytes collected per surgery. After collection, the oocytes were submitted to in vitro maturation for 24 h and stained with Hoechst 33342 dye to evaluate their nuclear status; 64.5% of the oocytes reached MII and 16.1% were spontaneously activated by parthenogenesis. The nuclear status of oocytes that did not undergo in vitro maturation was evaluated; 80.9% were found to be immature. NUPECCe - Núcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Departamento de Zootecnia Centro de Ciências Agrárias Universidade Federal da Paraíba Departamento de Medicina Veterinária Faculdade de Zootecnia e Engenharia de Alimentos Universidade de São Paulo NUPECCe - Núcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista

Made available in DSpace on 2018-12-11T17:04:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-12-01 The Amazonian brown brocket deer (Mazama nemorivaga) is an endemic species of the Amazon rainforest region, which has suffered constant threats due to hunting and increasing deforestation. Artificial insemination (AI), combined with genomic banks, is considered an important tool for maintaining conservation programs of endangered species; however, the number of live offspring born from AI in non-domesticated cervids is extremely low. Thus, studies designed to develop, adapt, or enhance AI techniques are of fundamental importance. This report describes a successful transposition of the cervix with semen deposition in the uterine lumen of a M. nemorivaga female, based on the transcervical AI technique used in sheep, as well as using specific tools develop for IA in small ruminants, which have resulted in the birth of a healthy male fawn. Department of Preventive Veterinary Medicine and Animal Reproduction College of Agricultural and Veterinary Sciences (FCAV) São Paulo State University (UNESP) Deer Research and Conservation Center (NUPECCE) Department of Animal Science FCAV-UNESP Department of Clinical and Veterinary Surgery FCAV-UNESP Department of Preventive Veterinary Medicine and Animal Reproduction College of Agricultural and Veterinary Sciences (FCAV) São Paulo State University (UNESP) Deer Research and Conservation Center (NUPECCE) Department of Animal Science FCAV-UNESP Department of Clinical and Veterinary Surgery FCAV-UNESP

Made available in DSpace on 2018-11-26T17:15:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01 Graduate Program in Zoology of the Federal University of Parana Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The ovarian cycle in howler monkeys (genus Alouatta) has beean investigated through several biological parameters (ranging between 16.3 and 29.5 days); however, no data exist concerning the ovarian activity of the southern brown howler monkey (Alouatta guariba clamitans). This study aimed to describe the ovarian cycle of A. g. clamitans by profiling fecal progestin concentrations. Over 20 weeks, fecal samples of eight captive adult females of A. g. clamitans were collected. The collections were made at dawn, 5 days a week, and the samples were frozen immediately following collection. Next, they were dried, pulverized and hormonal metabolites were extracted to determine progestin concentrations by enzyme immunoassay. Of the 758 samples tested, the mean concentration of fecal progestins was 2866.40 +/- 470.03 ng/g of dry feces, while the mean concentration at baseline was 814.47 +/- 164.36 ng/g of dry feces. Among the eight females, one showed no ovarian cyclicity and three presented periods of probable absence of cyclicity and low progestin concentrations. A mean duration of 16 +/- 0.52 days was observed for the 35 cycles studied. The interluteal phase lasted 4 +/- 0.37 days on average, with a mean concentration of fecal progestins of 467.98 +/- 29.12 ng/g of dry feces, while the luteal phase lasted 11 +/- 0.50 days, with a mean concentration of 4283.27 +/- 193.31 ng/g of dry feces. Besides describing the characteristics of the ovarian cycle, possible causes for the low concentrations of fecal progestins and periods of absence of cyclicity are also discussed. Univ Fed Parana, Dept Zool, Programa Posgrad Zool, Curitiba, Parana, Brazil Ctr Pesquisas Biol Indaial, Indaial, Brazil Univ Estadual Paulista, Dept Zootecnia, Nucleo Pesquisa & Conservacao Cervideos, Jaboticabal, Brazil Univ Reg Blumenau, Dept Ciencias Nat, Blumenau, Brazil Univ Estadual Paulista, Dept Zootecnia, Nucleo Pesquisa & Conservacao Cervideos, Jaboticabal, Brazil CNPq: 303757/2012-4 CNPq: 131196/2010-3

Made available in DSpace on 2018-11-26T17:44:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-25 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The marsh deer is an endangered species from the marshlands of central South America. This study aimed to characterize certain aspects of the reproductive physiology of marsh deer hinds, including the duration and fecal progestins profile of the estrous cycle, pregnancy and post-partum periods, and evaluate the effect of cloprostenol administration on this species. The experimental group consisted of six females and one fertile male marsh deer. During monitoring of the estrous cycle, the fresh fecal samples were collected daily and, during pregnancy, they were collected twice weekly. The hormonal profile obtained from daily fecal samples indicated that the mean duration of the estrous cycle was 21.3 +/- 1.3 days (6.4 days inter-luteal phase and 14.8 days luteal phase; n = 16 estrous cycles). The mean concentration of fecal progestins in the inter-luteal phase was 834 +/- 311 ng g(-1), in the luteal phase was 3979 +/- 1611 ng g(-1), value between them was 1457 ng g(-1). No significant difference in fecal estrogen concentrations was determined during the estrous cycle. The corpora luteum was not responsive to cloprostenol until Day 6 of the estrous cycle, the period previously described as the inter-luteal phase. Half the females became pregnant following treatment with cloprostenol and two others were fertilized in their natural estrous cycle. Four females delivered fawns, and the mean duration of pregnancy was 253 +/- 4 days. Fecal progestin concentrations were similar to those of the estrous cycle during the first 11 weeks of pregnancy and increased significantly (> 15250 ng g(-1)) thereafter, providing a presumptive diagnosis guideline. Within 60 days of post-partum analyses, 75% of the deer exhibited behavioural estrus and/or ovarian activity. This study generated a broader understanding of the marsh deer species concerning the production of consistent data related to its reproduction. This knowledge can be used to assist the reproductive management of this species and, consequently, to promote its conservation. Univ Estadual Paulista, Fac Ciencias Agr & Vet, NUPECCE Nucleo Pesquisa & Conservacao Cervideos, Deer Res & Conservat Ctr,Dept Zootecnia, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil Univ Estadual Paulista, Fac Ciencias Agr & Vet, NUPECCE Nucleo Pesquisa & Conservacao Cervideos, Deer Res & Conservat Ctr,Dept Zootecnia, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil

Lay summary: This study aimed to characterize some aspects of the reproductive physiology of female marsh deer, such as length and fecal progestins profile of estrous cycle, pregnancy and post parturition periods, information that we consider an important and decisive factor for the elaboration of conservation actions of Neotropical deer species., The marsh deer is an endangered species from the marshlands of central South America. This study aimed to characterize certain aspects of the reproductive physiology of marsh deer hinds, including the duration and fecal progestins profile of the estrous cycle, pregnancy and post-partum periods, and evaluate the effect of cloprostenol administration on this species. The experimental group consisted of six females and one fertile male marsh deer. During monitoring of the estrous cycle, the fresh fecal samples were collected daily and, during pregnancy, they were collected twice weekly. The hormonal profile obtained from daily fecal samples indicated that the mean duration of the estrous cycle was 21.3 ± 1.3 days (6.4 days inter-luteal phase and 14.8 days luteal phase; n = 16 estrous cycles). The mean concentration of fecal progestins in the inter-luteal phase was 834 ± 311 ng g−1, in the luteal phase was 3979 ± 1611 ng g−1, value between them was 1457 ng g−1. No significant difference in fecal estrogen concentrations was determined during the estrous cycle. The corpora luteum was not responsive to cloprostenol until Day 6 of the estrous cycle, the period previously described as the inter-luteal phase. Half the females became pregnant following treatment with cloprostenol and two others were fertilized in their natural estrous cycle. Four females delivered fawns, and the mean duration of pregnancy was 253 ± 4 days. Fecal progestin concentrations were similar to those of the estrous cycle during the first 11 weeks of pregnancy and increased significantly ( > 15250 ng g−1) thereafter, providing a presumptive diagnosis guideline. Within 60 days of post-partum analyses, 75% of the deer exhibited behavioural estrus and/or ovarian activity. This study generated a broader understanding of the marsh deer species concerning the production of consistent data related to its reproduction. This knowledge can be used to assist the reproductive management of this species and, consequently, to promote its conservation.

Made available in DSpace on 2018-11-26T15:44:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-06-01 The red brocket (Mazama americana) is a South American deer with a wide geographical distribution that presents different chromosomal variants depending on their location. At least six different cytotypes belonging to two distinct evolutionary lineages have been described. This study aimed to verify the existence of postzygotic reproductive isolation between cytotypes of M. americana by comparative evaluation of pure and hybrid males. Seven 18-month-old bucks were submitted to seminal collection and evaluation and testicle histological evaluations. The pure males showed normal parameters for sperm quality and testicular histology. Hybrids from the same evolutionary lineage (= 10 chromosomes different from progenitors) presented azoospermia and evidence of testicular degeneration. Despite the striking morphological similarities, we can conclude that populations with more distinct karyotypes possess an effective reproductive barrier; moreover, there is evidence that reproductive isolation mechanisms exist between some closer karyotypes, corroborating the hypothesis that M. americana is best characterized as a superspecies. Thus, the future description of several new species for this taxon is expected, since the tendency is to establish efficient mechanisms of postzygotic reproductive isolation, preventing the introgression and fusion of genomes from different populations through chromosome variation. Summary Sentence Differences equal to or greater than seven chromosomes produce an effective reproductive isolation between red brocket deer cytotypes and a depression in fertility occurs among karyotypes with at least two chromosome diferences. Univ Fed Piaui, Lab Histol & Embriol, Teresina, PI, Brazil Univ Estadual Paulista, Nucleo Pesquisa & Conservacao Cervideos, Jaboticabal, SP, Brazil Univ Fed Mato Grosso, Inst Ciencias Biol & Saude, Campus Univ Araguaia, Pontal Do Araguaia, MT, Brazil Univ Estadual Paulista, Nucleo Pesquisa & Conservacao Cervideos, Jaboticabal, SP, Brazil

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:51:03Z No. of bitstreams: 1 S0100-736X2012000700013.pdf: 438296 bytes, checksum: 686160eff988620c71bc07dd94aeb21c (MD5) Made available in DSpace on 2013-08-22T18:51:03Z (GMT). No. of bitstreams: 1 S0100-736X2012000700013.pdf: 438296 bytes, checksum: 686160eff988620c71bc07dd94aeb21c (MD5) Previous issue date: 2012-07-01 Made available in DSpace on 2013-09-30T18:02:51Z (GMT). No. of bitstreams: 2 S0100-736X2012000700013.pdf: 438296 bytes, checksum: 686160eff988620c71bc07dd94aeb21c (MD5) S0100-736X2012000700013.pdf.txt: 31316 bytes, checksum: 4bb554588b20d6bee2b4edd9f4072fec (MD5) Previous issue date: 2012-07-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:18:58Z No. of bitstreams: 2 S0100-736X2012000700013.pdf: 438296 bytes, checksum: 686160eff988620c71bc07dd94aeb21c (MD5) S0100-736X2012000700013.pdf.txt: 31316 bytes, checksum: 4bb554588b20d6bee2b4edd9f4072fec (MD5) Made available in DSpace on 2014-05-20T13:18:58Z (GMT). No. of bitstreams: 2 S0100-736X2012000700013.pdf: 438296 bytes, checksum: 686160eff988620c71bc07dd94aeb21c (MD5) S0100-736X2012000700013.pdf.txt: 31316 bytes, checksum: 4bb554588b20d6bee2b4edd9f4072fec (MD5) Previous issue date: 2012-07-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) O desenvolvimento de técnicas não invasivas para a obtenção de sêmen de cervídeos facilita a criação de bancos genômicos, que são importantes instrumentos para a conservação ex situ e in situ. Este trabalho teve como objetivo criar uma metodologia não-invasiva de coleta de sêmen e comparar duas técnicas de coleta em quatro espécies do gênero Mazama: M. americana, M. gouazoubira, M. nana e M. nemorivaga. Para tanto, foram utilizados seis machos (M) e duas fêmeas (F) da espécie M. americana, 3M e 2F de M. gouazoubira, 1M e 1F de M. nana e 2M e 1F de M. nemorivaga. Para cada técnica testada, foi realizado um período de habituação dos animais ao manejo. em seguida, duas técnicas de condicionamento e coleta foram avaliadas. Na primeira delas foi utilizada uma fêmea em estro com desvio lateral do pênis para vagina artificial (FEDL), obtendo-se a coleta de 50% dos indivíduos (100% dos machos de M. gouazoubira e 50% dos machos de M. americana), não obtendo ejaculados das demais espécies. Na segunda técnica, utilizando um manequim taxidermizado com urina de fêmea em estro (MUFE) não foi possível a coleta de nenhum ejaculado. em todas as fases foi observado o comportamento do macho quanto ao tempo de interesse e aproximação, reflexo de Flehmen, ato de cheirar ou lamber, exposição do pênis, ereção, número de falsas montas, tentativas de cópula e ocorrência de agressividade entre os animais. The development of noninvasive techniques for obtaining semen from deer facilitates the creation of genome banks, which are important tools for ex situ and in situ conservation. This study aimed to establish a noninvasive method of semen collection and compare two techniques of collection in four species of the genus Mazama: M. americana, M. gouazoubira, M. nana and M. nemorivaga. To achieve this, 6 males (M) and 2 females (F) of the species M. Americana, 3M and 2F of M. gouazoubira, 1M and 1F of M. nana and 2M and 1F of M. nemorivaga were used. For each technique tested, a period of habituation to animal handling was conducted; then, the two conditioning techniques and collection were evaluated. In the first, a female in estrus was used with lateral deviation of the penis to an artificial vagina (FEDL), yielding collection from 50% of the males (100% from M. gouazoubira and 50% from M. americana), with no ejaculate from the remaining species. In the second technique, using a taxidermized dummy with urine from females in estrus (MUFE), no semen collection was possible. During all stages, male behavior was observed regarding the time of interest and approximation, the Flehmen response, the act of sniffing or licking, exposure of the penis, erection, number of false mounts, attempts at copulation and the occurrence of aggression between the deer. Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Zootecnia Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Zootecnia FAPESP: 03/07904-0 FAPESP: 07/02593-8

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:50:45Z No. of bitstreams: 1 S0100-736X2008001100003.pdf: 155818 bytes, checksum: 5e8e8c1ae3fb50d221c6dea1eadff8d5 (MD5) Made available in DSpace on 2013-08-22T18:50:45Z (GMT). No. of bitstreams: 1 S0100-736X2008001100003.pdf: 155818 bytes, checksum: 5e8e8c1ae3fb50d221c6dea1eadff8d5 (MD5) Previous issue date: 2008-11-01 Made available in DSpace on 2013-09-30T18:02:02Z (GMT). No. of bitstreams: 2 S0100-736X2008001100003.pdf: 155818 bytes, checksum: 5e8e8c1ae3fb50d221c6dea1eadff8d5 (MD5) S0100-736X2008001100003.pdf.txt: 31814 bytes, checksum: 76ab2ec19dbbedcc0126052e8748b91e (MD5) Previous issue date: 2008-11-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:14:13Z No. of bitstreams: 2 S0100-736X2008001100003.pdf: 155818 bytes, checksum: 5e8e8c1ae3fb50d221c6dea1eadff8d5 (MD5) S0100-736X2008001100003.pdf.txt: 31814 bytes, checksum: 76ab2ec19dbbedcc0126052e8748b91e (MD5) Made available in DSpace on 2014-05-20T13:14:13Z (GMT). No. of bitstreams: 2 S0100-736X2008001100003.pdf: 155818 bytes, checksum: 5e8e8c1ae3fb50d221c6dea1eadff8d5 (MD5) S0100-736X2008001100003.pdf.txt: 31814 bytes, checksum: 76ab2ec19dbbedcc0126052e8748b91e (MD5) Previous issue date: 2008-11-01 Item merged in doublecheck by Felipe Arakaki (fe.arakaki@gmail.com) on 2015-11-18T10:48:10Z Item was identical to item(s): 1650, 1660 at handle(s): http://hdl.handle.net/11449/1744, http://hdl.handle.net/11449/1754 A laparoscopia ainda é pouco utilizada como ferramenta para técnicas de reprodução assistida em cervídeos sul-americanos, não havendo informações sobre seus efeitos e protocolos anestésicos seguros para sua realização. Objetivaramse avaliar as possíveis alterações na freqüência cardíaca (FC), respiratória (FR), saturação de oxihemoglobina (SpO2) e temperatura retal (TR) durante a laparoscopia para visualização dos órgãos reprodutivos de seis fêmeas de veado-catingueiro (Mazama gouazoubira) anestesiadas com a associação cetamina (5mg/kg), xilazina (0,3mg/kg), midazolam (0,5mg/kg) e isofluorano. Cada animal, após anestesiado, foi posicionado em decúbito dorsal para realização de duas laparoscopias com insuflação abdominal de CO2 (14,2 ± 2,39mmHg; M ± EPM) com intervalo de 40 dias. Para avaliar os principais eventos da laparoscopia, esta foi dividida em três períodos: animal sem insuflação abdominal (P1), com insuflação abdominal (P2) e insuflação abdominal com os quadris elevados a 45º (P3). O controle foi realizado após 40 dias da última laparoscopia, para isto, cada animal foi novamente anestesiado e mantido em decúbito dorsal por um período de tempo igual ao tempo médio de duração das anestesias realizadas nas laparoscopias, sem que o procedimento laparoscópico fosse realizado. O tempo de anestesia dos controles foi também dividido em P1, P2 e P3, respeitando o tempo médio de duração de cada um destes períodos das laparoscopias. Para análise dos dados foi usado o teste de análise de variância (ANOVA) seguido do teste de Tukey e valores de P

Made available in DSpace on 2014-12-03T13:11:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-03-04Bitstream added on 2014-12-03T13:22:36Z : No. of bitstreams: 1 WOS000334458900002.pdf: 1785302 bytes, checksum: 67376de2e4b2cb31a6e4a38aec22f56a (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Background: The red brocket deer, Mazama americana, has at least six distinct karyotypes in different regions of South America that suggest the existence of various species that are today all referred to as M. americana. From an evolutionary perspective, the red brockets are a relatively recent clade that has gone through intense diversification. This study sought to prove the existence of post-zygotic reproductive isolation in deer offspring between distinct chromosome lineages. To achieve this, inter-cytotype and intra-cytotype crosses were performed, which resulted in both F1 hybrid (n = 5) and pure offspring (n = 3) in captivity.Results: F1 females were analyzed in terms of their karyotypes, ovarian histology, estrous cycles and in vitro embryo production. Pure females presented parameters that were similar to those previously reported for M. Americana; however, the parameters for hybrid females were different. Two hybrids were determined to be sterile, while the remaining hybrids presented characteristics of subfertility.Conclusions: The results support the existence of well-established reproductive isolation among the most distant karyotype lineages and elucidates the need to define all karyotype variants and their geographical ranges in order to define the number of species of red brocket. Univ Estadual Paulista, UNESP, FCAV, Dept Zootecnia,NUPECCE Nucl Pesquisa & Conservaca, BR-14884900 Jaboticabal, SP, Brazil UNESP, FCAV, Programa Posgrad Med Vet Reproducao Anim, BR-14884900 Jaboticabal, SP, Brazil Fac Vet Med, Lab Embryol & Biotechn Reprod, BR-91501970 Porto Alegre, RS, Brazil Univ Estadual Paulista, UNESP, FCAV, Dept Zootecnia,NUPECCE Nucl Pesquisa & Conservaca, BR-14884900 Jaboticabal, SP, Brazil UNESP, FCAV, Programa Posgrad Med Vet Reproducao Anim, BR-14884900 Jaboticabal, SP, Brazil

Made available in DSpace on 2014-12-03T13:11:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-03-19Bitstream added on 2014-12-03T13:22:36Z : No. of bitstreams: 1 WOS000334703000001.pdf: 385890 bytes, checksum: 461e9e117c2e56211fe64f5764411b18 (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: Stress is a limiting factor in assisted reproduction in wild animals maintained in captivity. However, the knowledge of assisted reproduction techniques for wild animals is useful for future in situ and ex situ conservation programs. Thus, this study evaluated the ovulation rate, presence of functional corpora lutea and fecal glucocorticoid levels following treatments promoting superovulation in captive brown brocket deer.Methods: The crossover design used six hinds, allocated to two groups (n = 6): eCG Treatment, CIDR for 8 days, followed by 0.25 mg of EB on day 0, 700 IU of eCG on day 4 following device insertion and 265 mug of PGF2alfa on day 8; and FSH Treatment, CIDR for 7.5 days, followed by 0.25 mg of EB on day 0, 130 mg of FSH in 8 equal doses and 265 mug of PGF2alfa on day 7.5. Induced adrenal activity and treatment efficacy were evaluated by corpora lutea (CL) counts and fecal glucocorticoid and progestin concentration (ng/g feces) analyses for five different phases: Pre, two days before treatment; Early, first four days of treatment; Late, last four days of treatment; Total, entire treatment period; and Post, five days posttreatment.Results: eCG Treatment resulted in the highest number of CL (P lower than 0.05). There was no significant difference for fecal glucocorticoid concentrations in five different time periods between the treatments; however Pre fecal glucocorticoid concentrations (90.06+/-19.64) were significantly different from Late (200.76+/-26.39) within FSH Treatment. The mean fecal progestin concentration and mean ovulation rate were higher in eCG Treatment (4293.69+/-769.47, 7.0+/-1.8) than in FSH Treatment (1571.26+/-240.28, 2.6+/-0.8) (P lower than or equal to 0.05).Conclusions: Although the eCG Treatment induced a good superovulatory response, with the formation of functional corpora lutea, we cannot yet affirm that we have established a suitable protocol for induction of SOV in the species M. gouazoubira because approximately 65% of the deer showed premature regression of the corpora lutea. Moreover, multiple FSH applications in FSH Treatment resulted in a low ovulation rate and induced an increase in fecal glucocorticoid levels. Univ Estadual Paulista, Fac Ciencias Agra & Vet, Deer Res & Conservat Ctr, Dept Zootecnia,NUPECCE,Nuleo Pesquisa & Conservac, BR-14884900 Jaboticabal, SP, Brazil Univ Estadual Paulista, Fac Ciencias Agra & Vet, Deer Res & Conservat Ctr, Dept Zootecnia,NUPECCE,Nuleo Pesquisa & Conservac, BR-14884900 Jaboticabal, SP, Brazil