9 results on '"Emmie Dumont"'
Search Results
2. Multimodal biomarker discovery for active Onchocerca volvulus infection.
- Author
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Ole Lagatie, Emmanuel Njumbe Ediage, Dirk Van Roosbroeck, Stijn Van Asten, Ann Verheyen, Linda Batsa Debrah, Alex Debrah, Maurice R Odiere, Ruben T'Kindt, Emmie Dumont, Koen Sandra, Lieve Dillen, Tom Verhaeghe, Rob Vreeken, Filip Cuyckens, and Lieven J Stuyver
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Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
The neglected tropical disease onchocerciasis, or river blindness, is caused by infection with the filarial nematode Onchocerca volvulus. Current estimates indicate that 17 million people are infected worldwide, the majority of them living in Africa. Today there are no non-invasive tests available that can detect ongoing infection, and that can be used for effective monitoring of elimination programs. In addition, to enable pharmacodynamic studies with novel macrofilaricide drug candidates, surrogate endpoints and efficacy biomarkers are needed but are non-existent. We describe the use of a multimodal untargeted mass spectrometry-based approach (metabolomics and lipidomics) to identify onchocerciasis-associated metabolites in urine and plasma, and of specific lipid features in plasma of infected individuals (O. volvulus infected cases: 68 individuals with palpable nodules; lymphatic filariasis cases: 8 individuals; non-endemic controls: 20 individuals). This work resulted in the identification of elevated concentrations of the plasma metabolites inosine and hypoxanthine as biomarkers for filarial infection, and of the urine metabolite cis-cinnamoylglycine (CCG) as biomarker for O. volvulus. During the targeted validation study, metabolite-specific cutoffs were determined (inosine: 34.2 ng/ml; hypoxanthine: 1380 ng/ml; CCG: 29.7 ng/ml) and sensitivity and specificity profiles were established. Subsequent evaluation of these biomarkers in a non-endemic population from a different geographical region invalidated the urine metabolite CCG as biomarker for O. volvulus. The plasma metabolites inosine and hypoxanthine were confirmed as biomarkers for filarial infection. With the availability of targeted LC-MS procedures, the full potential of these 2 biomarkers in macrofilaricide clinical trials, MDA efficacy surveys, and epidemiological transmission studies can be investigated.
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- 2021
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3. Multimodal biomarker discovery for active Onchocerca volvulus infection
- Author
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Ann Verheyen, Emmie Dumont, Emmanuel Njumbe Ediage, Dirk Van Roosbroeck, Maurice R. Odiere, Lieven J. Stuyver, Ole Lagatie, Alexander Yaw Debrah, Filip Cuyckens, Lieve Dillen, Ruben T'Kindt, Tom Verhaeghe, Koen Sandra, Linda Batsa Debrah, Stijn Van Asten, and Rob J. Vreeken
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Male ,Nematoda ,Physiology ,Metabolite ,RC955-962 ,Glycobiology ,Urine ,Onchocerciasis ,Biochemistry ,Mass Spectrometry ,Plasma ,chemistry.chemical_compound ,Medical Conditions ,Arctic medicine. Tropical medicine ,Medicine and Health Sciences ,Metabolites ,Biomarker discovery ,Lymphatic filariasis ,education.field_of_study ,biology ,Eukaryota ,Nucleosides ,Lipids ,Glycosylamines ,Body Fluids ,Infectious Diseases ,Helminth Infections ,Biomarker (medicine) ,Female ,Onchocerca ,Anatomy ,Public aspects of medicine ,RA1-1270 ,Research Article ,Neglected Tropical Diseases ,Population ,Macrofilaricide ,Helminths ,Parasitic Diseases ,medicine ,Animals ,Metabolomics ,Humans ,education ,business.industry ,Organisms ,Public Health, Environmental and Occupational Health ,Biology and Life Sciences ,Tropical Diseases ,Lipid Metabolism ,biology.organism_classification ,medicine.disease ,Invertebrates ,Onchocerca volvulus ,Inosine ,Metabolism ,chemistry ,Immunology ,business ,Zoology ,Biomarkers ,Chromatography, Liquid - Abstract
The neglected tropical disease onchocerciasis, or river blindness, is caused by infection with the filarial nematode Onchocerca volvulus. Current estimates indicate that 17 million people are infected worldwide, the majority of them living in Africa. Today there are no non-invasive tests available that can detect ongoing infection, and that can be used for effective monitoring of elimination programs. In addition, to enable pharmacodynamic studies with novel macrofilaricide drug candidates, surrogate endpoints and efficacy biomarkers are needed but are non-existent. We describe the use of a multimodal untargeted mass spectrometry-based approach (metabolomics and lipidomics) to identify onchocerciasis-associated metabolites in urine and plasma, and of specific lipid features in plasma of infected individuals (O. volvulus infected cases: 68 individuals with palpable nodules; lymphatic filariasis cases: 8 individuals; non-endemic controls: 20 individuals). This work resulted in the identification of elevated concentrations of the plasma metabolites inosine and hypoxanthine as biomarkers for filarial infection, and of the urine metabolite cis-cinnamoylglycine (CCG) as biomarker for O. volvulus. During the targeted validation study, metabolite-specific cutoffs were determined (inosine: 34.2 ng/ml; hypoxanthine: 1380 ng/ml; CCG: 29.7 ng/ml) and sensitivity and specificity profiles were established. Subsequent evaluation of these biomarkers in a non-endemic population from a different geographical region invalidated the urine metabolite CCG as biomarker for O. volvulus. The plasma metabolites inosine and hypoxanthine were confirmed as biomarkers for filarial infection. With the availability of targeted LC-MS procedures, the full potential of these 2 biomarkers in macrofilaricide clinical trials, MDA efficacy surveys, and epidemiological transmission studies can be investigated., Author summary Today’s diagnosis of infection with the filarial parasite Onchocerca volvulus mainly depends on the microscopic analysis of skin biopsies and serological testing. The work presented here describes the use of multiple mass spectrometry-based screening methods (metabolomics and lipidomics) to search for biomarkers indicative of infection with Onchocerca volvulus. This resulted in the identification of elevated concentrations of the plasma metabolites inosine and hypoxanthine as biomarkers for filarial infection, and of the urine metabolite cis-cinnamoylglycine as biomarker for O. volvulus. Further evaluation of these biomarkers in a geographically distinct non-endemic population however invalidated the use of urine cis-cinnamoylglycine. These findings are of utmost importance as it not only opens new avenues in the development of non-invasive diagnostic tools for filarial infections, but also emphasizes the need for evaluation and validation of newly discovered biomarkers in different populations from different geographies.
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- 2021
4. Cov-MS
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Dan Lane, Sigrid Verhelst, Maarten Dhaenens, Amy C. Harms, Griet Debyser, Nicolas Drouin, Johannes P. C. Vissers, Lize Cuypers, Katleen Van Uytfanghe, Dieter Deforce, Stuart A. Oehrle, Catherine S. Lane, Jan Claereboudt, Péter Judák, Nathan Debunne, Sally Hannam, Lennart Martens, Pathmanaban Ramasamy, Robbin Bouwmeester, Andrea Bhangu-Uhlmann, N. Leigh Anderson, Laurence Van Oudenhove, Nick Morrice, Sven Degroeve, Laura Corveleyn, Marc Cherlet, Peter Van Eenoo, Morteza Razavi, Tim Van Den Bossche, Evelien Wynendaele, Ruben t’Kindt, Said El Ouadi, Emmie Dumont, Nikunj Tanna, Bart De Spiegeleer, Laura De Clerck, Katrien Lagrou, Surya Gupta, Tim Reyns, Thomas Hankemeier, Pankaj Gupta, Christophe P. Stove, Bart Van Puyvelde, Donald J. L. Jones, Florian C. Sigloch, Simon Daled, Sander Willems, Olivier Tytgat, Ralf Gabriels, Jean-Baptiste Vincendet, Laurie De Wilde, Geert A. Martens, Steve Silvester, K. Roels, Koen Sandra, Department of Bio-engineering Sciences, Faculty of Sciences and Bioengineering Sciences, Pathology/molecular and cellular medicine, and Diabetes Pathology & Therapy
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Proteomics ,Coronavirus disease 2019 (COVID-19) ,Computer science ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Chemistry, Multidisciplinary ,Economic shortage ,Spreading ,Computational biology ,Rising population density ,infectious diseases ,Protein detection ,Article ,Mass Spectrometry ,reverse transcription polymerase chain reaction ,03 medical and health sciences ,Viral Proteins ,Medicine and Health Sciences ,Global mobility ,QD1-999 ,Diagnostics ,030304 developmental biology ,Community based ,0303 health sciences ,Science & Technology ,Pandemic ,Biochemistry, Genetics and Molecular Biology(all) ,SARS-CoV-2 ,030302 biochemistry & molecular biology ,COVID-19 ,Diagnostic test ,global mobility ,QUANTIFICATION ,3. Good health ,Chemistry ,Physical Sciences ,MRM - Abstract
Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, the virus that causes COVID-19, spread so rapidly across the globe. The policy response to such pandemics will always have to include accurate monitoring of the spread, as this provides one of the few alternatives to total lockdown. However, COVID-19 diagnosis is currently performed almost exclusively by reverse transcription polymerase chain reaction (RT-PCR). Although this is efficient, automatable, and acceptably cheap, reliance on one type of technology comes with serious caveats, as illustrated by recurring reagent and test shortages. We therefore developed an alternative diagnostic test that detects proteolytically digested SARS-CoV-2 proteins using mass spectrometry (MS). We established the Cov-MS consortium, consisting of 15 academic laboratories and several industrial partners to increase applicability, accessibility, sensitivity, and robustness of this kind of SARS-CoV-2 detection. This, in turn, gave rise to the Cov-MS Digital Incubator that allows other laboratories to join the effort, navigate, and share their optimizations and translate the assay into their clinic. As this test relies on viral proteins instead of RNA, it provides an orthogonal and complementary approach to RT-PCR using other reagents that are relatively inexpensive and widely available, as well as orthogonally skilled personnel and different instruments. Data are available via ProteomeXchange with identifier PXD022550. ispartof: JACS AU vol:1 issue:6 pages:750-765 ispartof: location:United States status: published
- Published
- 2021
5. LC fractionation followed by pyrolysis GC–MS for the in-depth study of aroma compounds formed during tobacco combustion
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Nobuo Ochiai, Hirotoshi Tamura, Kazuhisa Mitsui, Frank David, Pat Sandra, and Emmie Dumont
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Chromatography ,biology ,Chemistry ,Pyrolysis gc ms ,food and beverages ,Fraction (chemistry) ,Fractionation ,Mass spectrometry ,Combustion ,biology.organism_classification ,Analytical Chemistry ,Fuel Technology ,Organic chemistry ,Tobacco leaf extract ,Pyrolysis ,Aroma - Abstract
Analytical pyrolysis combined with gas chromatography–mass spectrometry (Py-GC–MS) is an effective technique for studying combustion processes such as cigarette smoking. Direct pyrolysis of tobacco leaf results in a very complex mixture of organic compounds containing volatile, semi-volatile and non-volatile compounds. Consequently, detecting and identifying the main tobacco aroma compounds and their formation pathways are very difficult. An innovative workflow that can elucidate the aroma compounds formed during the combustion of tobacco is presented. The workflow consists of LC fractionation of a tobacco leaf extract, recombination of fractions in a fraction omission approach and Py-GC–MS. Using multivariate statistical data analysis, the fractions that contribute most to the formation of typical cigarette smoke aromas could be identified. In addition, GC–MS analysis of derivatized LC fractions could be used to identify the precursors of these aroma compounds. The analytical approach is suitable to correlate the effect of different classes of compounds in tobacco to the typical aroma compounds in cigarette smoke.
- Published
- 2015
6. Biomarkers of human exposure to personal care products : results from the Flemish Environment and Health Study (FLEHS 20072011)
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Adrian Covaci, Ilse Loots, Emmie Dumont, Greet Schoeters, Vera Nelen, Tinne Geens, Elly Den Hond, Bert Morrens, Liesbeth Bruckers, Willy Baeyens, Melissa Paulussen, Benoit Nemery de Bellevaux, Frank David, and Nicolas Van Larebeke
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Adult ,Male ,Hydroxybenzoic acid ,Environmental Engineering ,Adolescent ,Tetrahydronaphthalenes ,Urinary system ,Population ,Parabens ,Cosmetics ,Soaps ,chemistry.chemical_compound ,Young Adult ,Belgium ,Environmental health ,Biomonitoring ,Environmental Chemistry ,Medicine ,Humans ,Benzopyrans ,Galaxolide ,education ,Waste Management and Disposal ,Biology ,education.field_of_study ,business.industry ,Pharmacology. Therapy ,Environmental Exposure ,Pollution ,language.human_language ,Triclosan ,Flemish ,Chemistry ,chemistry ,Human exposure ,language ,Female ,business ,Biomarkers - Abstract
Personal care products (PCPs), such as soaps, perfumes, cosmetics, lotions, etc., contain a variety of chemicals that have been described as potentially hormone disrupting chemicals. Therefore, it is important to assess the internal exposure of these chemicals in humans. Within the 2nd Flemish Environment and Health Study (FLEHS II, 2007–2011), the human exposure to three classes of pollutants that are present in a wide variety of PCPs – i.e. polycyclic musks (galaxolide, HHCB and tonalide, AHTN in blood), parabens (urinary para -hydroxybenzoic acid, HBA) and triclosan (urinary TCS) – was assessed in 210 Flemish adolescents (14–15 years) and in 204 adults (20–40 years) randomly selected from the general population according to a stratified two stage clustered study design. The aim of this study was to define average levels of exposure in the general Flemish population and to identify determinants of exposure. Average levels (GM (95% CI)) in the Flemish adolescents were 0.717 (0.682–0.753) μg/L for blood HHCB; 0.118 (0.108–0.128) μg/L for blood AHTN; 1022 (723–1436) μg/L for urinary HBA and 2.19 (1.64–2.92) μg/L for urinary TCS. In the adults, levels of HBA were on average 634 (471–970) μg/L. Inter-individual variability was small for HHCB and AHTN, intermediate for HBA, and large for TCS. All biomarkers were positively associated with the use of PCPs. Additionally, levels of HHCB and AHTN increased with higher educational level of the adolescents. Both in adults and adolescents, urinary HBA levels were negatively correlated with BMI. We define here Flemish exposure values for biomarkers of PCPs, which can serve as baseline exposure levels to identify exposure trends in future biomonitoring campaigns.
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- 2013
7. Selenium speciation from food source to metabolites: a critical review
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Rita Cornelis, Emmie Dumont, and Frank Vanhaecke
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Ecology ,chemistry.chemical_element ,Biology ,Biochemistry ,food.food ,Food Analysis ,Analytical Chemistry ,Body Fluids ,Selenium ,food ,Metabolism ,chemistry ,Genetic algorithm ,Humans ,Identification (biology) ,Tissue Distribution ,Tissue distribution ,Literature study ,Brazil nut - Abstract
Especially in the last decade, a vast number of papers on Se and its role in health issues have been published. This review gives a brief, critical overview of the main analytical findings reported in these papers. Of particular interest is the Se content in different food sources worldwide and the extent to which their consumption is reflected in the Se content of human tissues and body fluids. Several food sources, both natural (Brazil nuts, garlic, Brassica juncea) and Se-enriched (yeast-based supplements), are discussed as to origin, characteristics, Se metabolism and impact of their consumption on the human body. The continuous development of new and improvement of existing analytical techniques has provided different powerful tools to unravel the Se species and their function. An up-to-date literature study on Se speciation analysis is given, illustrating how analytical chemistry in its different facets aids in the identification of Se compounds and provides insight into the complete metabolic pathway of Se throughout the human body. This review includes a detailed image of the current state-of-the-art of Se speciation analysis in these food sources and in human tissues and body fluids.
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- 2006
8. Liquid chromatography-mass spectrometry (LC-MS): a powerful combination for selenium speciation in garlic (Allium sativum)
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Frank Vanhaecke, Kazuo Suzuki, Yasumitsu Ogra, Emmie Dumont, and Rita Cornelis
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Electrospray ,Chemical ionization ,Chromatography ,Time Factors ,Chemistry ,Ion chromatography ,Reversed-phase chromatography ,Tandem mass spectrometry ,Mass spectrometry ,Biochemistry ,Sensitivity and Specificity ,Mass Spectrometry ,Analytical Chemistry ,Selenium ,Liquid chromatography–mass spectrometry ,Organoselenium Compounds ,Sample preparation ,Garlic ,Chromatography, Liquid - Abstract
Liquid chromatography (LC) hyphenated with both elemental and molecular mass spectrometry has been used for Se speciation in Se-enriched garlic. Different species were separated by ion-pair liquid chromatography-inductively coupled plasma mass spectrometry (LC-ICP-MS) after hot-water extraction. They were identified by on-line reversed-phase liquid chromatography-electrospray ionization tandem mass spectrometry (RPLC-ESI-MS-MS). Se-methionine and Se-methylselenocysteine were determined by monitoring their product ions. Another compound, gamma-glutamyl-Se-methylselenocysteine, shown to be the most abundant form of Se in the garlic, was determined without any additional sample pre-treatment after extraction and without the need for a synthesized standard. Product ions for this dipeptide were detected by LC-ESI-MS-MS for three isotopes of Se-78 Se, 80Se: and 82Se. The method was extended to the species extracted during in-vitro gastrointestinal digestion. Because both Se-methylselenocysteine and gamma-glutamyl-Se-methylselenocysteine have anticarcinogenic properties, their extractability and stability during human digestion are very important. Garlic was also treated with saliva, to enable detection and analysis of species extracted during mastication. Detailed information on the extractability of selenium species by both simulated gastric and intestinal fluid are given, and variation of the distribution of Se among the different species with time is discussed. Although the main species in garlic is the dipeptide gamma-glutamyl-Se-methylselenocysteine, Se-methylselenocysteine is the main compound present in the extracts after treatment with gastrointestinal fluids. Two more, so far unknown compounds were observed in the chromatogram. The extracted species and their transformations were analysed by combining LC-ICP-MS and LC-ESI-MS-MS. In both the simulated gastric and intestinal digests, Se-methionine, Se-methylselenocysteine, and gamma-glutamyl-Se-methylselenocysteine could be determined by LC-ESI-MS-MS by measuring their typical product ions.
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- 2005
9. Hyphenated techniques for speciation of Se in in vitro gastrointestinal digests of Saccharomyces cerevisiae
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Emmie Dumont, Rita Cornelis, and Frank Vanhaecke
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Electrospray ,Chromatography ,Gastric Juice ,Intestinal Secretions ,Chemistry ,Spectrophotometry, Atomic ,Proteolytic enzymes ,Saccharomyces cerevisiae ,Mass spectrometry ,Tandem mass spectrometry ,Biochemistry ,High-performance liquid chromatography ,Yeast ,Mass Spectrometry ,Analytical Chemistry ,Selenocysteine ,Selenium ,Dietary Supplements ,Sample preparation ,Digestion ,Selenomethionine ,Inductively coupled plasma mass spectrometry ,Chromatography, High Pressure Liquid - Abstract
A method was developed allowing the separation, detection and identification of Se species extracted from yeast supplements during simulated digestion processes. The in vitro gastric and intestinal digests were studied for their Se compounds by successive high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) and high-performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ES-MS-MS) analyses. The conditions for the separation were chosen as to be compatible with both ICP-MS and ES-MS-MS detection. HPLC-ICP-MS was used to screen the extracts for their Se content. By means of HPLC-ES-MS-MS, the compounds extracted were identified on-line according to their retention time, m/ z of the molecular ion and the presence of typical product ions. From these results, it was clear that the main compound extracted by both gastric and intestinal fluid was Se-methionine, which was also the main Se compound extracted by proteolytic digestion from the yeast supplements. Two other minor compounds could be identified as Se-cystine and Se(O)-methionine, a degradation product of Se-methionine.
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- 2004
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