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This study evaluates proximate, antinutrient, and antioxidant activities of raw and processed quinoa flour (RQF and PQF). Product development and sensory evaluation were also addressed to provide prospects for human consumption. Methods: Quinoa was processed by two methods - soaking and roasting. Some nutrients like moisture, ash, protein, carbohydrate, crude fibre, fat, iron, calcium, vitamin C, phytic acid, saponins, total phenol, and 2,2-diphenylpicrylhydrazyl (DPPH) were analyzed. The recipes were made and conduct acceptability evaluation by a 9-point hedonic scale. The mean, standard deviation, and One-Way Analysis of Variance (proximate, antinutrient, antioxidant) were utilized as the statistical approach for the analysis of data. Results: The study revealed that some nutrient composition of RQF was found to be impressive with moisture (11%), ash (2.1%), protein (16.6%), crude fibre (2%), iron (11.6%), and calcium (76.1%) as compared to PQF; however, carbohydrate (69.9%), fat (9.6%), and vitamin C (9.1%) was increased in PQF, and phytic acid and saponins were decreased in all the PQF. DPPH’ free antioxidant potential was observed that RQF lowered free radical scavenging activity. In all PQF, the total phenol content was both raised and reduced. Also, proximate and antioxidant activity showed significant differences (P0.05). The two recipes were prepared as upma (soaked for 48 h), and chakli (roasted at 145 °C). Sample B of upma (50% semolina with 50% soaked quinoa flour) and sample A of chakli (75% refined flour with 25% roasted quinoa flour) were more acceptable. Conclusion: This study concluded that the use of domestic processing of quinoa seeds decreases some nutrient value compared with RQF and also infers the dietary importance of quinoa.

A 34-day-old, male Bully pup was presented in veterinary clinics with an uncertain anamnestic history of anorexia, lethargy, abdominal pain, and discomfort. On clinical examination, the pup had pale conjunctiva with a pot-bellied appearance and expelled cream-colored roundworms in feces. Blood and fecal samples were collected and analyzed. Hemoglobin and PCV values were lower, depicting anemia. A qualitative and quantitative examination of the fecal sample was carried out and results showed infection with Toxocara canis with fecal egg count (FEC) of 3200/gram of feces. Gross and light microscopic examination revealed the presence of adults of Toxocara canis with distinctive morphological features. Apropos, the pup was treated with a combination of pyrantel pamoate and fenbendazole orally @10mg/kg body weight, repeated after 14 days. The pup recovered successfully, as evidenced by decreased FECs, increased weight gain, and a high hematocrit score.

Novel N-(4-alkyl-4-oxo-1,3-thiazolidin-3-yl)-2-(5-nitro-1H-benzimidazole-1-yl)acetamide derivatives were evaluated as M. tuberculosis transcription inhibitors using protein 3Q3S, by performing molecular docking and molecular dynamics studies. Twelve promising candidates exhibiting good binding interactions in the form of hydrogen bonds, pi-cation interactions, pi-pi stacking and low binding energies (-7.576 kcal/mol to -5.038 kcal/mol) were selected for wet lab synthesis. Their invitro anti-tubercular activity tests using Microplate Alamar Blue Assay were compared with insilico studies. Compounds 4a, 4b, and 4g have exhibited good activity with MIC values of 1.6 μg/ml, while the other compounds exhibited activity at MIC values of 3.125-6.25 μg/ml. The results show that the presence of an additional H-bonding group at the ortho position in the substituted aryl group attached to the thiazolidine ring is leading to an increase in the activity owing to an increase in the binding interaction of the molecule to the substrate.

In the rapidly evolving landscape of continuous electrocardiogram (ECG) monitoring systems, there is a heightened demand for non-invasive sensors capable of measuring ECGs and detecting heart rate variability (HRV) in diverse populations, ranging from cardiovascular patients to sports enthusiasts. Challenges like device accuracy, patient privacy, signal noise, and long-term safety impede the use of wearable devices in clinical practice. This scoping review aims to assess the performance and safety of novel multi-channel, sensor-based biopotential wearable devices in adults. A comprehensive search strategy was employed on four databases, resulting in 143 records and the inclusion of 12 relevant studies. Most studies focused on healthy adult subjects (n = 6), with some examining controlled groups with atrial fibrillation (AF) (n = 3), long QT syndrome (n = 1), and sleep apnea (n = 1). The investigated bio-sensor devices included chest-worn belts (n = 2), wrist bands (n = 2), adhesive chest strips (n = 2), and wearable textile smart clothes (n = 4). The primary objective of the included studies was to evaluate device performance in terms of accuracy, signal quality, comparability, and visual assessment of ECGs. Safety findings, reported in five articles, indicated no major side effects for long-term/continuous monitoring, with only minor instances of skin irritation. Looking forward, there are ample opportunities to enhance and test these technologies across various physical activity intensities and clinical conditions.

Stellaria media Linn., a member of the family Caryophyllaceae, is generally known by the name of Chickweed. This plant is extensively cultivated globally and is inherent to Africa, Asia, China, Europe, and North America. It is a well-known medicinal plant with immense therapeutic uses. Nutritional studies have revealed the presence of protein, especially 16 amino acids, vitamins, and minerals such as calcium, iron, phosphorus, and zinc. Phytochemicals, mainly flavonoids, isoflavonoids, saponins, tannins, alkaloids, phenolic acids, triterpenoids, phenolic compounds, and anthraquinone are present in chickweed. It has multiple therapeutic potentials like anti-obesity, anti-diabetic, anti-fungal, anti-bacterial, anti-inflammatory, anti-leishmanial, anti-anxiety, and toxicity profiles. The crude extracts and their metabolites did not show any toxicity in the experimental animal. This review summarizes the nutritional, phytochemical, pharmacological, and toxicity studies on this plant concerning its future use in pharmacological drugs.

Abstract

Antimony (Sb) is introduced into soils, sediments, and aquatic environments from various sources such as weathering of sulfide ores, leaching of mining wastes, and anthropogenic activities. High Sb concentrations are toxic to ecosystems and potentially to public health via the accumulation in food chain. Although Sb is poisonous and carcinogenic to humans, the exact mechanisms causing toxicity still remain unclear. Most studies concerning the remediation of soils and aquatic environments contaminated with Sb have evaluated various amendments that reduce Sb bioavailability and toxicity. However, there is no comprehensive review on the biogeochemistry and transformation of Sb related to its remediation. Therefore, the present review summarizes: (1) the sources of Sb and its geochemical distribution and speciation in soils and aquatic environments, (2) the biogeochemical processes that govern Sb mobilization, bioavailability, toxicity in soils and aquatic environments, and possible threats to human and ecosystem health, and (3) the approaches used to remediate Sb-contaminated soils and water and mitigate potential environmental and health risks. Knowledge gaps and future research needs also are discussed. The review presents up-to-date knowledge about the fate of Sb in soils and aquatic environments and contributes to an important insight into the environmental hazards of Sb. The findings from the review should help to develop innovative and appropriate technologies for controlling Sb bioavailability and toxicity and sustainably managing Sb-polluted soils and water, subsequently minimizing its environmental and human health risks.

Proboscis lateralis (PL) is a rare congenital malformation of the craniofacial structure with varied clinical associations. None of the studies documented a discrete review of ophthalmic presentations in PL. The principal aim of the present study is to explore the ophthalmic manifestations of PL. The ancillary goal is to derive a relationship between congenital deformity in PL and various ophthalmic anomalies. Databases were searched in order to obtain articles related to PL. A qualitative systematic analysis of 100 subjects was performed. In PL, eyelid coloboma (32.6%) is the most common ocular feature, followed by hypertelorism (25.3%), iris coloboma (22.4%), lacrimal system abnormality (20.7%), malpositioned eyebrow (14.4%) and retinochoroidal coloboma (12.9%). Sinonasal deformity is the most common systemic abnormality, detected in 87.9% of cases of PL, as compared with central nervous system involvement (56.2%) and other anomalies. The analysis showed a strong significant association between brain abnormalities and hypertelorism (p=0.000) and between brain abnormalities and micro-ophthalmia/anophthalmia (p=0.000). Statistically significant association was noted between cumulative ocular abnormalities and cumulative systemic abnormalities (p=0.001). The present study on PL reviewed the salient features of this rare congenital disorder. The study outcome provides a new aspect to concomitant ocular abnormalities. This study supports the view that other congenital anomalies in cases of PL had significant influence on certain ophthalmic anomalies.

Single nucleotide polymorphism (SNP) at codon 167, 198 and 200 of β-tubulin isotype 1 gene accounts for benzimidazole resistance in Haemonchus contortus. To assess the anthelmintic resistance to benzimidazole group, allele specific polymerase chain reaction (AS-PCR) was employed on 50 adults of local abattoir of Ludhiana and 138 harvested larvae of H. contortus from faecal samples collected from field flocks of 6 districts of western zone of Punjab. The adults were found to be homozygous resistant (rr) showing 100% prevalence of resistant allele (r). AS-PCR on 138 larvae collected from different districts of Punjab showed 49.28% (69) homozygous resistant (rr), 3.62% (5) homozygous susceptible (SS) and 46.37% (64) heterozygous (rS) population. Species was confirmed as H. contortus by PCR-RFLP from the three fragments of size 462 bp, 211 bp and 147 bp. The pioneer study on the molecular diagnosis of benzimidazole resistance in H. contortus showed an overall allele frequency for resistant and susceptible population to be 0.72 (TTC) and 0.28 (TAC), espectively indicating development of high level of resistance in sheep.

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disorder with synovitis and articular pathology as its primary expositions. Leflunomide (Lfd) is an anti-rheumatic drug that is effective in the treatment of RA, but displays severe side effects upon prolonged systemic administration. Local therapy might represent a promising strategy to treat rheumatoid arthritis without eliciting systemic adverse effects. In this study, leflunomide-loaded poly(ε-caprolactone) nanoparticles (Lfd-NPs) were prepared and assessed as a local drug delivery system capable of alleviating RA-associated inflammation. Lfd-NPs were optimized using the Quality by Design (QbD) approach, applying a 32 full factorial design. In vitro drug release from NPs was examined in simulated synovial fluid. In addition, the in vivo efficacy of Lfd-NPs was evaluated in the Adjuvant Induced Arthritis (AIA) rodent model. Sustained drug release in simulated synovial fluid was observed for up to 168 h. A gradual reduction in paw volume and knee diameter was observed over the course of treatment, indicating the regression of the disease. In addition, significant reductions in serum proinflammatory markers and cytokines, including the C-reactive protein (CRP), rheumatoid factor (RF), TNF-α, IL1-β, and IL-6, were verified upon treatment with Lfd-NPs, suggesting the modulation of immune responses at the pathological site. Most importantly, no remarkable signs of toxicity were observed in Lfd-NP-treated animals. Collectively, intra-articularly administered Lfd-NPs might represent a potential therapeutic alternative to systemically administered drugs for the treatment of rheumatoid arthritis, without eliciting systemic adverse effects.

Retinal cavernous hemangiomas is a rare vascular tumor, isolated and non-progressive. This tumor is incidentally diagnosed on fundus examination and visual impairment can be rare presentation from vitreous hemorrhage, pre-retinal traction, hyphema, or macular scarring. The tumor has typical appearance of grape-like clusters of dilated vascular sacs with variable surface gliosis. Characteristic imaging findings aids in diagnosis. Newly emerged imaging tool is optical coherence tomography angiography (OCT A) which obtains high-resolution visualization of retinal vasculature in non-invasive fashion. In the present case report, we analyzed various diagnostic tool available for retinal cavernous hemangiomas; illustrating on the OCT A features.

The paradigm shift in our understanding of the dynamic, multifactorial nature of dental caries and the resultant change in caries preventive and treatment strategies necessitates that caries risk assessment (CRA) should be an integral part of any caries management protocol. This review discusses the rationale for CRA and the role various risk indicators play in the fluctuating demineralization-remineralization cycle of dental caries. It also provides an overview of different CRA methods and a risk-based clinical protocol for dental caries management in infants and children.

In certain clinical scenarios, using an intact natural tooth which is in good clinical condition as pontic for interim duration could offer benefits such as excellent color, shape, and size match, positive psychological value, minimal cost, and minimum chairside time with no laboratory procedure involved. Natural tooth pontic suitably modified and bonded to adjacent teeth enables proper healing in the area without compromising the anterior esthetic demands of the patient. In the present study, a clinical technique for immediate interim tooth replacement is being presented, utilizing the clinical crown to assist the clinician in providing an esthetically acceptable treatment option.

A study was conducted to determine the diagnostic role of p63 in breast FNAC sample for proper identification of myoepithelial cells. The sample comprised of 51 patients with breast lesions who have undergone FNAC and subsequently biopsy. Following conventional smear preparations, the entire materials fixed and processed for histopathology staining. The cell blocks were embedded in paraffin, sectioned and processed for Immunostaining. As p63 is a reliable nuclear marker of myoepithelial cells in the breast, it was used to distinguish these cells from their mimics in FNABs. Benign lesions usually contained p63 positive myoepithelial cells, and it was demonstrated that it may be a useful marker for highlighting these cells. p63 positive cells were also observed frequently in samples of DCIS and IDC, although, based on careful cytomorphologic evaluation, they may have been classified correctly as malignant cells. Hence, based on previously published data and on our findings, we advocate that anti-p63 antibodies may be used to identify myoepithelial cells as well as to overcome the cytomorphologic distortion of myoepithelial cells in FNABs of the breast.

Objectives: To evaluate the effect of Mifepristone (25 mg) on symptomatic myoma in perimenopausal women. Study Design: Open label clinical trial. Materials and Methods: Ninety three perimenopausal women of age 35-50 years having symptomatic myoma were selected from Gynecology OPD and given 25 mg Mifepristone once daily continuously for three months. Variables as; baseline uterine size, uterine volume, myoma size, volume, their number, position, characteristics, hemoglobin and blood parameters, were taken and followed monthly for six months. Bleeding and pain scores were checked on monthly visits. Changes in above parameters were tabulated during the first three months treatment phase and then next three post-treatment phase for analysis. Statistical Analysis: Was done by calculating mean, standard deviation, standard error and percentage distribution of variables. Results: Menorrhagia was the most common symptom which led patients to report to hospital. Mean uterine volume reduced to 63.69% of baseline, Mean dominant Myoma volume reduced to 53.62% and hemoglobin level raised to 137% after complete three months of treatment. Changes persisted in next three months post-treatment follow-up, while hysterectomy was required in 10 (12.2%) cases. Conclusion: Three months treatment of 25 mg Mifepristone effectively controls bleeding, reduces the uterine and myoma volume and thus can avoid blood transfusion and hysterectomy in a lot of symptomatic myoma cases.

Aim: To assess the effectiveness of outreach program in the 12 years school going children during a three year follow up period of Lucknow city. Materials and Methods: A random sample of 298 school going children of age 12 years was drawn from adopted schools of Lucknow. Three years later the same population was reexamined with a population of 242 children of 15 y age group. The oral health status of these children was accessed by using WHO 1997 profoma. Results: Significantly (p

Background: This study evaluates the proximate, antinutrient, and antioxidant activities of raw and processed quinoa flour (RQF and PQF). Product development and sensory evaluation were also addressed to provide prospects for human consumption. Methods: Quinoa was processed by two methods - soaking and roasting. Some nutrients like moisture, ash, protein, carbohydrate, crude fibre, fat, iron, calcium, vitamin C, phytic acid, saponins, total phenol, and 2,2-diphenylpicrylhydrazyl (DPPH) were analyzed. The recipes were made and conduct acceptability evaluation by a 9-point hedonic scale. The mean, standard deviation, and One-Way Analysis of Variance were utilized as the statistical approach for the analysis of data. Results: The study revealed that some nutrient composition of RQF was found to be impressive with moisture (11%), ash (2.1%), protein (16.6%), crude fibre (2%), iron (11.6%), and calcium (76.1%) as compared to PQF; however, carbohydrate (69.9%), fat (9.6%), and vitamin C (9.1%) was increased in PQF, and phytic acid and saponins were decreased in all the PQF. DPPH free antioxidant potential was observed that RQF lowered free radical scavenging activity. In all PQF, the total phenol content was both raised and reduced. Also, proximate and antioxidant activity showed significant differences (P<0.05), and antinutrient analysis showed no significant differences (P>0.05). The two recipes were prepared as upma (soaked for 48 h), and chakli (roasted at 145 °C). Sample B of upma (50% semolina with 50% soaked quinoa flour) and sample A of chakli (75% refined flour with 25% roasted quinoa flour) were more acceptable. Conclusion: This study concluded that the use of domestic processing of quinoa seeds decreases some nutrient value compared with RQF and also infers the dietary importance of quinoa. [ABSTRACT FROM AUTHOR]