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1. Inorganic phosphate modifies stationary phase fitness and metabolic pathways in Lactiplantibacillus paraplantarum CRL 1905

Author: Mario Araoz, Mariana Grillo-Puertas, Alejandra de Moreno de LeBlanc, Elvira María Hebert, Josefina María Villegas, and Viviana Andrea Rapisarda
Subjects: lactic acid bacteria, stationary phase, polyphosphate, proteomics, biofilm, Microbiology, QR1-502
Abstract: Inorganic phosphate (Pi) concentration modulates polyphosphate (polyP) levels in diverse bacteria, affecting their physiology and survival. Lactiplantibacillus paraplantarum CRL 1905 is a lactic acid bacterium isolated from quinoa sourdough with biotechnological potential as starter, for initiating fermentation processes in food, and as antimicrobial-producing organism. The aim of this work was to evaluate the influence of the environmental Pi concentration on different physiological and molecular aspects of the CRL 1905 strain. Cells grown in a chemically defined medium containing high Pi (CDM + P) maintained elevated polyP levels up to late stationary phase and showed an enhanced bacterial survival and tolerance to oxidative stress. In Pi sufficiency condition (CDM-P), cells were ~ 25% longer than those grown in CDM + P, presented membrane vesicles and a ~ 3-fold higher capacity to form biofilm. Proteomic analysis indicated that proteins involved in the “carbohydrate transport and metabolism” and “energy production and conversion” categories were up-regulated in high Pi stationary phase cells, implying an active metabolism in this condition. On the other hand, stress-related chaperones and enzymes involved in cell surface modification were up-regulated in the CDM-P medium. Our results provide new insights to understand the CRL 1905 adaptations in response to differential Pi conditions. The adjustment of environmental Pi concentration constitutes a simple strategy to improve the cellular fitness of L. paraplantarum CRL 1905, which would benefit its potential as a microbial cell factory.
Published: 2024
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2. Oral administration of a probiotic Lactobacillus modulates cytokine production and TLR expression improving the immune response against Salmonella enterica serovar Typhimurium infection in mice

Author: Perdigón Gabriela, Castillo Natalia A, and de Moreno de LeBlanc Alejandra
Subjects: Microbiology, QR1-502
Abstract: Abstract Background Diarrheal infections caused by Salmonella, are one of the major causes of childhood morbidity and mortality in developing countries. Salmonella causes various diseases that range from mild gastroenteritis to enteric fever, depending on the serovar involved, infective dose, species, age and immune status of the host. Probiotics are proposed as an attractive alternative possibility in the prevention against this pathogen infection. Previously we demonstrated that continuous Lactobacillus casei CRL 431 administration to BALB/c mice before and after challenge with Salmonella enterica serovar Typhimurium (S. Typhimurium) decreased the severity of Salmonella infection. The aim of the present work was to deep into the knowledge about how this probiotic bacterium exerts its effect, by assessing its impact on the expression and secretion of pro-inflammatory (TNFα, IFNγ) and anti-inflammatory (IL-10) cytokines in the inductor and effector sites of the gut immune response, and analyzing toll-like receptor (TLR2, TLR4, TLR5 and TLR9) expressions in both healthy and infected mice. Results Probiotic administration to healthy mice increased the expression of TLR2, TLR4 and TLR9 and improved the production and secretion of TNFα, IFNγ and IL-10 in the inductor sites of the gut immune response (Peyer's patches). Post infection, the continuous probiotic administration, before and after Salmonella challenge, protected the host by modulating the inflammatory response, mainly in the immune effector site of the gut, decreasing TNFα and increasing IFNγ, IL-6 and IL-10 production in the lamina propria of the small intestine. Conclusions The oral administration of L. casei CRL 431 induces variations in the cytokine profile and in the TLRs expression previous and also after the challenge with S. Typhimurium. These changes show some of the immune mechanisms implicated in the protective effect of this probiotic strain against S. Typhimurium, providing an alternative way to reduce the severity of the infection.
Published: 2011
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3. Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice

Author: Carmuega Esteban, de Moreno de LeBlanc Alejandra, Núñez Ivanna, Galdeano Carolina, Weill Ricardo, and Perdigón Gabriela
Subjects: Diseases of the digestive system. Gastroenterology, RC799-869
Abstract: Abstract Background Malnutrition affects the immune response, causing a decrease of defence mechanisms and making the host more susceptible to infections. Probiotics can reconstitute the intestinal mucosa and stimulate local and systemic immunity. The aim of this work was evaluate the effects of a probiotic fermented milk as a complement of a re-nutrition diet, on the recovery of the intestinal barrier, and mucosal and systemic immune functions in a murine model of non-severe protein-energy-malnutrition. Its potential protection against Salmonella enterica serovar Typhimurium (S. Typhimurium) infection was also analyzed. Methods Mice were undernourished and divided into 3 groups according to the dietary supplement received during re-nutrition (milk, probiotic fermented milk or its bacterial free supernatant) and compared to well-nourished and malnourished mice. They were sacrificed previous to the re-nutrition and 5 days post re-nutrition. The phagocytic activity of macrophages from spleen and peritoneum and the changes in the intestinal histology and microbiota were evaluated. Different immune cell populations and cytokine productions were analyzed in the small intestine tissues. The effect of the re-nutrition supplements on the systemic immunity using OVA antigen and against an infection with S. Typhimurium was also studied. Results Probiotic fermented milk was the most effective re-nutrition diet that improved the intestinal microbiota. Its administration also increased the number of IgA+ cells, macrophages and dendritic cells. The production of different cytokine (IFN-γ, TNF-α, IL-12) by these cells and the phagocytic activity in peritoneum and spleen was also increased. This re-nutrition diet also stimulated the systemic immune response against OVA antigen which was diminished after the malnutrition period and also improved the host response against S. Typhimurium, decreasing the spread of pathogenic bacteria to the liver and the spleen. The importance of the metabolites released during milk fermentation was also demonstrated through the analysis of the bacterial free supernatant obtained from the probiotic fermented milk, but the whole product showed the best effects in the parameters evaluated in this study. Conclusions The administration of probiotic fermented milk as a dietary supplement during the re-nutrition process in a murine immunodeficiency model by malnutrition could be a good adjuvant diet to improve the gut and systemic immune response for the protection against Salmonella infection.
Published: 2011
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4. Effect of the administration of a fermented milk containing Lactobacillus casei DN-114001 on intestinal microbiota and gut associated immune cells of nursing mice and after weaning until immune maturity

Author: Carmuega Esteban, Galdeano Carolina, Dogi Cecilia A, de Moreno de LeBlanc Alejandra, Weill Ricardo, and Perdigón Gabriela
Subjects: Immunologic diseases. Allergy, RC581-607
Abstract: Abstract Background Microbial colonization of the intestine after birth is an important step for the development of the gut immune system. The acquisition of passive immunity through breast-feeding may influence the pattern of bacterial colonization in the newborn. The aim of this work was to evaluate the effect of the administration of a probiotic fermented milk (PFM) containing yogurt starter cultures and the probiotic bacteria strain Lactobacillus casei DN-114001 to mothers during nursing or their offspring, on the intestinal bacterial population and on parameters of the gut immune system. Results Fifteen mice of each group were sacrificed at ages 12, 21, 28 and 45 days. Large intestines were taken for determination of intestinal microbiota, and small intestines for the study of secretory-IgA (S-IgA) in fluid and the study of IgA+ cells, macrophages, dendritic cells and goblet cells on tissue samples. The consumption of the PFM either by the mother during nursing or by the offspring after weaning modified the development of bifidobacteria population in the large intestine of the mice. These modifications were accompanied with a decrease of enterobacteria population. The administration of this PFM to the mothers improved their own immune system and this also affected their offspring. Offspring from mice that received PFM increased S-IgA in intestinal fluids, which mainly originated from their mother's immune system. A decrease in the number of macrophages, dendritic cells and IgA+ cells during the suckling period in offspring fed with PFM was observed; this could be related with the improvement of the immunity of the mothers, which passively protect their babies. At day 45, the mice reach maturity of their own immune system and the effects of the PFM was the stimulation of their mucosal immunity. Conclusion The present work shows the beneficial effect of the administration of a PFM not only to the mothers during the suckling period but also to their offspring after weaning and until adulthood. This effect positively improved the intestinal microbiota that are related with a modulation of the gut immune response, which was demonstrated with the stimulation of the IgA + cells, macrophages and dendritic cells.
Published: 2008
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5. Streptomyces genus as a source of probiotics and its potential for its use in health

Author: Cuozzo, S., de Moreno de LeBlanc, A., LeBlanc, J.G., Hoffmann, N., and Tortella, G.R.
Published: 2023
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6. Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex

Author: Célia Chamignon, Geoffroy Mallaret, Julie Rivière, Marthe Vilotte, Sead Chadi, Alejandra de Moreno de LeBlanc, Jean Guy LeBlanc, Frédéric Antonio Carvalho, Marco Pane, Pierre-Yves Mousset, Philippe Langella, Sophie Lafay, and Luis G. Bermúdez-Humarán
Subjects: leaky gut, chronic diseases, barrier functions, probiotics, Lactobacillus, Microbiology, QR1-502
Abstract: Intestinal barrier integrity is essential in order to maintain the homeostasis of mucosal functions and efficient defensive reactions against chemical and microbial challenges. An impairment of the intestinal barrier has been observed in several chronic diseases. The gut microbiota and its impact on intestinal homeostasis is well described and numerous studies suggest the ability of some probiotic strains to protect the intestinal epithelial integrity and host homeostasis. In this work, we aimed to assess the beneficial effects of three Lactobacillus strains (Lacticaseibacillus rhamnosus LR04, Lacticaseibacillus casei LC03, and Lactiplantibacillus plantarum CNCM I-4459) and their mechanism of action in low-grade inflammation or neonatal maternal separation models in mice. We compared the impact of these strains to that of the well-known probiotic Lacticaseibacillus rhamnosus GG. Our results demonstrated that the three strains have the potential to restore the barrier functions by (i) increasing mucus production, (ii) restoring normal permeability, and (iii) modulating colonic hypersensitivity. Moreover, gene expression analysis of junctional proteins revealed the implication of Claudin 2 and Cingulin in the mechanisms that underlie the interactions between the strains and the host. Taken together, our data suggest that LR04, CNCM I-4459, and LC03 restore the functions of an impaired intestinal barrier.
Published: 2023
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7. Inorganic phosphate modifies stationary phase fitness and metabolic pathways in Lactiplantibacillus paraplantarum CRL 1905

Author: Araoz, Mario, primary, Grillo-Puertas, Mariana, additional, de Moreno de LeBlanc, Alejandra, additional, Hebert, Elvira María, additional, Villegas, Josefina María, additional, and Rapisarda, Viviana Andrea, additional
Published: 2024
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8. Maternal dietary omega-3 deficiency worsens the deleterious effects of prenatal inflammation on the gut-brain axis in the offspring across lifetime

Author: Leyrolle, Q., Decoeur, F., Briere, G., Amadieu, C., Quadros, A. R. A. A., Voytyuk, I., Lacabanne, C., Benmamar-Badel, A., Bourel, J., Aubert, A., Sere, A., Chain, F., Schwendimann, L., Matrot, B., Bourgeois, T., Grégoire, S., Leblanc, J. G., De Moreno De Leblanc, A., Langella, P., Fernandes, G. R., Bretillon, L., Joffre, C., Uricaru, R., Thebault, P., Gressens, P., Chatel, J. M., Layé, S., and Nadjar, A.
Published: 2021
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9. Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex

Author: Chamignon, Célia, primary, Mallaret, Geoffroy, additional, Rivière, Julie, additional, Vilotte, Marthe, additional, Chadi, Sead, additional, de Moreno de LeBlanc, Alejandra, additional, LeBlanc, Jean Guy, additional, Carvalho, Frédéric Antonio, additional, Pane, Marco, additional, Mousset, Pierre-Yves, additional, Langella, Philippe, additional, Lafay, Sophie, additional, and Bermúdez-Humarán, Luis G., additional
Published: 2023
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10. Oral administration of milk fermented by Lactobacillus casei CRL431 was able to decrease metastasis from breast cancer in a murine model by modulating immune response locally in the lungs

Author: Virginia Emilce Mendez Utz, Gabriela Perdigón, and Alejandra de Moreno de LeBlanc
Subjects: Probiotic, Lung metastasis, Breast cancer, Immune response, Nutrition. Foods and food supply, TX341-641
Abstract: The host immune system is an important factor to combat metastasis. In this work, it was evaluated the immunomodulation exerted by milk fermented with the probiotic strain Lactobacillus casei CRL431 (PFM) when was administered to mice in the metastatic stage of breast cancer. Mice received PFM were compared to animals given milk. PFM administration diminished metastasis in the lungs and increased the survival of the animals. Systemically, PFM administration decreased pro-inflammatory cytokines and locally in the lungs (metastatic organs) decreased F4/80+ cells, principally IL-10/F4/80+ cells. The highest percentages for CD4+ and CD4+CD8+ cells were observed in PFM group. Finally, immune cells from lungs of mice given PFM showed lower production of most cytokines evaluated when they were re-stimulated in vitro with tumour cells. The results obtained suggest that modulation of immune cells in the lungs by PFM could be a strategy to fight against tumour cells in the metastatic sites.
Published: 2019
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11. Tropical fruit by-products water extracts as sources of soluble fibres and phenolic compounds with potential antioxidant, anti-inflammatory, and functional properties

Author: Marcela Albuquerque Cavalcanti de Albuquerque, Romina Levit, Carolina Beres, Raquel Bedani, Alejandra de Moreno de LeBlanc, Susana Marta Isay Saad, and Jean Guy LeBlanc
Subjects: Probiotic, Fruit by-products, Dietary fibre, Phenolic compounds, Anti-inflammatory effect, Folate, Nutrition. Foods and food supply, TX341-641
Abstract: Fibre content, phenolic content, antioxidant and anti-inflammatory activities were evaluated for water extracts of fruit by-products (passion fruit, orange, acerola, and mango). The impact of these extracts on microbial growth, folate production, and adhesion ability of Streptococcus thermophilus TH-4 and Lactobacillus rhamnosus LGG was investigated. Mango water extract (MWE) presented the highest phenolic content and antioxidant activity. Orange water extract (OWE) and MWE showed the best anti-inflammatory potential by decreasing the highest nitric oxide levels. When TH-4 and LGG were grown together, folate production was only stimulated by MWE. Passion fruit water extract and OWE increased the TH-4 adhesion whereas acerola water extracts and MWE improved LGG adhesion when strains were used individually. These results showed that fruit by-product water extracts (FWE), especially from mango, presented potential beneficial biological and functional properties. These FWE could be used to develop new functional antioxidant foods and natural pharmaceutical ingredients.
Published: 2019
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12. The Ability of Riboflavin-Overproducing Lactiplantibacillus plantarum Strains to Survive Under Gastrointestinal Conditions

Author: Annel M. Hernández-Alcántara, Sandra Pardo, Mari Luz Mohedano, Graciela M. Vignolo, Alejandra de Moreno de LeBlanc, Jean Guy LeBlanc, Rosa Aznar, and Paloma López
Subjects: lactic acid bacteria (LAB), riboflavin, protein mCherry, Lactiplantibacillus plantarum, tolerance to gastrointestinal stress of Lactiplantibacillus, Microbiology, QR1-502
Abstract: Riboflavin, vitamin B2, is essential for humans and has to be obtained from the diet. Some lactic acid bacteria (LAB) produce this vitamin, and they can be used for in-situ fortification of foods. This could be an alternative to supplementation with chemically synthesized vitamin, to palliate riboflavin deficiencies in specific groups of people. Moreover, if the producing LAB could survive in the gastrointestinal stress (GIT) they could be added as probiotics in this environment. In the present study we tested two riboflavin-overproducing Lactiplantibacillus plantarum strains (M5MA1-B2 and M9MG6-B2), spontaneous mutants of LAB isolated from chicha, a traditional Andean beverage. These two LAB, and also their isogenic strains M5MA1-B2[pRCR12] and M9MG6-B2[pRCR12], expressing the mCherry protein from the pRCR12 plasmid, were evaluated in vitro under simulated GIT conditions. Among other, specifically developed protein fluorescence assays were used. The four LAB showed similar levels of adhesion (>6.0%) to Caco-2 cells, higher than that of the probiotic Lacticaseibacillus rhamnosus GG strain (4.51%). Thus, LAB biofilm formation was assessed in the labeled cells by intracellular mCherry fluorescence and in the unlabeled parental strains by crystal violet staining. Both methods detected the formation of consistent biofilms by the L. plantarum strains. The quantification of mCherry fluorescence was also used to analyze LAB auto-aggregation properties. High levels of auto-aggregation were detected for both M5MA1-B2[pRCR12] and M9MG6-B2[pRCR12]. Survival of LAB included in a commercial cereal-based food matrix (Incaparina) under GIT conditions was also evaluated. The four LAB were resistant in vitro to the stomach and intestinal stresses, and proliferated in this environment, indicating a protective and nutritional effect of the Incaparina on the bacteria. Also, M9MG6-B2 survival in the presence or absence of Incaparina was evaluated in vivo in a BALB/c mouse model. The administration of the M9MG6-B2 strain alone or together with Incaparina had no adverse effect on the health, growth and/or well-being of the rodents. In addition, an increment in the villus length/crypt depth ratio was observed. The overall results obtained indicate that the LAB studied have probiotic characteristics of interest for the development of functional foods.
Published: 2020
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13. Evaluation of the bioavailability and intestinal effects of milk fermented by folate producing lactic acid bacteria in a depletion/repletion mice model

Author: Ana Clara C. Cucick, Katia Gianni, Svetoslav D. Todorov, Alejandra de Moreno de LeBlanc, JeanGuy LeBlanc, and Bernadette D.G.M. Franco
Subjects: Folate, Vitamin deficiency, Lactic acid bacteria, Bioavailability, Fermented milk, Nutrition. Foods and food supply, TX341-641
Abstract: This study aimed to evaluate the bioavailability and effects on the intestinal mucosa of a bioenriched fermented milk (BFM) prepared with folate-producing lactic acid bacteria, using a depletion/repletion BALB/c mice model. Five Streptococcus thermophilus strains were combined with Lactobacillus plantarum 16cv and the combination that resulted in the best folate production was used for preparation of BFM. The folate content in BFM, prepared under controlled conditions (pH 6.0, 42 °C, 70 rpm, 24 h), was 321.1 ± 14.1 ng/mL. Results for mice fed with BFM were compared to those fed with other diets. The BFM group presented an increase in hemoglobin, hematocrit and red blood cells, and the villi height/crypt depth ratio was similar to that of animals receiving milk supplemented with commercial folic acid. The strains were sensitive to most tested antibiotics and lacked virulence genes, indicating that consumption of BFM may be a promising alternative to increase intake of folate.
Published: 2020
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14. Probiotics and Trained Immunity

Author: Naima G. Cortes-Perez, Alejandra de Moreno de LeBlanc, Jorge G. Gomez-Gutierrez, Jean Guy LeBlanc, and Luis G. Bermúdez-Humarán
Subjects: probiotics, trained immunity, immune response, human health, Microbiology, QR1-502
Abstract: The characteristics of innate immunity have recently been investigated in depth in several research articles, and original findings suggest that innate immunity also has a memory capacity, which has been named “trained immunity”. This notion has revolutionized our knowledge of the innate immune response. Thus, stimulation of trained immunity represents a therapeutic alternative that is worth exploring. In this context, probiotics, live microorganisms which when administered in adequate amounts confer a health benefit on the host, represent attractive candidates for the stimulation of trained immunity; however, although numerous studies have documented the beneficial proprieties of these microorganisms, their mechanisms of action are not yet fully understood. In this review, we propose to explore the putative connection between probiotics and stimulation of trained immunity.
Published: 2021
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15. Use of genetically modified lactic acid bacteria and bifidobacteria as live delivery vectors for human and animal health

Author: Romina Levit, Naima G. Cortes-Perez, Alejandra de Moreno de Leblanc, Jade Loiseau, Anne Aucouturier, Philippe Langella, Jean Guy LeBlanc, and Luis G. Bermúdez-Humarán
Subjects: Microbiology (medical), Vaccines, Infectious Diseases, Lactobacillales, Genetic Vectors, Gastroenterology, Animals, Humans, Bifidobacterium, Microbiology, Gastrointestinal Microbiome
Abstract: There is now strong evidence to support the interest in using lactic acid bacteria (LAB)in particular, strains of lactococci and lactobacilli, as well as bifidobacteria, for the development of new live vectors for human and animal health purposes. LAB are Gram-positive bacteria that have been used for millennia in the production of fermented foods. In addition, numerous studies have shown that genetically modified LAB and bifodobacteria can induce a systemic and mucosal immune response against certain antigens when administered mucosally. They are therefore good candidates for the development of new mucosal delivery strategies and are attractive alternatives to vaccines based on attenuated pathogenic bacteria whose use presents health risks. This article reviews the most recent research and advances in the use of LAB and bifidobacteria as live delivery vectors for human and animal health.
Published: 2022

16. Use of genetically modified lactic acid bacteria and bifidobacteria as live delivery vectors for human and animal health

Author: Levit, Romina, primary, Cortes-Perez, Naima G., additional, de Moreno de Leblanc, Alejandra, additional, Loiseau, Jade, additional, Aucouturier, Anne, additional, Langella, Philippe, additional, LeBlanc, Jean Guy, additional, and Bermúdez-Humarán, Luis G., additional
Published: 2022
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17. Streptomyces genus as a source of probiotics and its potential for its use in health

Author: S. Cuozzo, A. de Moreno de LeBlanc, J.G. LeBlanc, N. Hoffmann, and G.R. Tortella
Subjects: Probiotics, Microbiota, Animals, Microbiology, Streptomyces, Gastrointestinal Microbiome, Anti-Bacterial Agents
Abstract: The effect of a probiotic on gut microbiota depends not only on the species of microorganism but specifically on the strain. In human beings, as in other animals, specific probiotics have been associated with numerous beneficial properties, which include weight modulation (gain or loss), immune modulation, and prevention of many disorders such as lactose intolerance, cardiovascular diseases, and antibiotic-associated diarrhoea. Streptomyces are an essential group of soil bacteria in the Actinomycetes family. They are related to producing a wide range of secondary metabolites known for their beneficial effects on human health. However, according to the human microbiome analysis, a lower prevalence of Streptomyces genus exists than in other non-human microbiomes. This difference can be associated with current lifestyles. In this article, we review the benefits associated with different compounds produced by Streptomyces, with a particular focus on the production of exopolysaccharides, antibiotics, and other secondary metabolites and the potential innovative use of Streptomyces spp. as probiotics.
Published: 2022

18. The ability of Riboflavin-overproducing Lactiplantibacillus plantarum strains to survive under gastrointestinal conditions

Author: Ministerio de Ciencia, Innovación y Universidades (España), Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo, European Cooperation in Science and Technology, Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Mohedano Bonillo, Mari Luz [0000-0001-6748-9443], Vignolo, Graciela [0000-0003-2368-9050], De Moreno de LeBlanc, A. [0000-0003-0352-374X], Leblanc, Jean Guy [0000-0002-4634-8630], Aznar, Rosa [0000-0002-8761-8887], López, Paloma [0000-0001-8755-8952], Hernández-Alcántara, Annel M., Pardo, S., Mohedano Bonillo, Mari Luz, Vignolo, Graciela, De Moreno de LeBlanc, A., Leblanc, Jean Guy, Aznar, Rosa, López García, Paloma, Ministerio de Ciencia, Innovación y Universidades (España), Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo, European Cooperation in Science and Technology, Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Mohedano Bonillo, Mari Luz [0000-0001-6748-9443], Vignolo, Graciela [0000-0003-2368-9050], De Moreno de LeBlanc, A. [0000-0003-0352-374X], Leblanc, Jean Guy [0000-0002-4634-8630], Aznar, Rosa [0000-0002-8761-8887], López, Paloma [0000-0001-8755-8952], Hernández-Alcántara, Annel M., Pardo, S., Mohedano Bonillo, Mari Luz, Vignolo, Graciela, De Moreno de LeBlanc, A., Leblanc, Jean Guy, Aznar, Rosa, and López García, Paloma
Abstract: Riboflavin, vitamin B2, is essential for humans and has to be obtained from the diet. Some lactic acid bacteria (LAB) produce this vitamin, and they can be used for in-situ fortification of foods. This could be an alternative to supplementation with chemically synthesized vitamin, to palliate riboflavin deficiencies in specific groups of people. Moreover, if the producing LAB could survive in the gastrointestinal stress (GIT) they could be added as probiotics in this environment. In the present study we tested two riboflavin-overproducing Lactiplantibacillus plantarum strains (M5MA1-B2 and M9MG6-B2), spontaneous mutants of LAB isolated from chicha, a traditional Andean beverage. These two LAB, and also their isogenic strains M5MA1-B2[pRCR12] and M9MG6-B2[pRCR12], expressing the mCherry protein from the pRCR12 plasmid, were evaluated in vitro under simulated GIT conditions. Among other, specifically developed protein fluorescence assays were used. The four LAB showed similar levels of adhesion (>6.0%) to Caco-2 cells, higher than that of the probiotic Lacticaseibacillus rhamnosus GG strain (4.51%). Thus, LAB biofilm formation was assessed in the labeled cells by intracellular mCherry fluorescence and in the unlabeled parental strains by crystal violet staining. Both methods detected the formation of consistent biofilms by the L. plantarum strains. The quantification of mCherry fluorescence was also used to analyze LAB auto-aggregation properties. High levels of auto-aggregation were detected for both M5MA1-B2[pRCR12] and M9MG6-B2[pRCR12]. Survival of LAB included in a commercial cereal-based food matrix (Incaparina) under GIT conditions was also evaluated. The four LAB were resistant in vitro to the stomach and intestinal stresses, and proliferated in this environment, indicating a protective and nutritional effect of the Incaparina on the bacteria. Also, M9MG6-B2 survival in the presence or absence of Incaparina was evaluated in vivo in a BALB/c mouse model. The adm
Published: 2020

19. Evaluation of vitamin-producing and immunomodulatory lactic acid bacteria as a potential co-adjuvant for cancer therapy in a mouse model

Author: A. de Moreno de LeBlanc, Romina Levit, G. Savoy de Giori, and Jean Guy LeBlanc
Subjects: medicine.medical_treatment, Riboflavin, Applied Microbiology and Biotechnology, law.invention, chemistry.chemical_compound, Probiotic, Mice, law, Lactobacillales, Intestinal Mucosa, 0303 health sciences, Mice, Inbred BALB C, biology, PROBIOTIC, IMMUNOMODULATION, General Medicine, Vitamins, Lactic acid, Cytokines, Female, Fluorouracil, Adjuvant, Biotechnology, Vitamin, Mucositis, Cell Survival, Cancer therapy, Antineoplastic Agents, Breast Neoplasms, Microbiology, Cell Line, Immunomodulation, 03 medical and health sciences, Folic Acid, Adjuvants, Immunologic, medicine, INTESTINAL MUCOSITIS, Animals, Humans, 030304 developmental biology, purl.org/becyt/ford/2.11 [https], 030306 microbiology, business.industry, biology.organism_classification, BREAST TUMOUR, B vitamins, Disease Models, Animal, chemistry, purl.org/becyt/ford/2 [https], Caco-2 Cells, B VITAMINS, business, Bacteria
Abstract: Aims: To evaluate a mixture of selected lactic acid bacteria (LAB) (a riboflavin-producer, a folate-producer and an immunomodulatory strain) as co-adjuvant for 5-fluorouracil (5-FU) chemotherapy in cell culture and using a 4T1 cell animal model of breast cancer. Methods and results: The viability of Caco-2 cells exposed to 5-FU and/or LAB was analysed. Mice bearing breast tumour were treated with 5-FU and/or LAB. Tumour growth was measured. Intestinal mucositis (IM) was evaluated in small intestine; haematological parameters and plasma cytokines were determined. The bacterial mixture did not negatively affect the cytotoxic activity of 5-FU on Caco-2 cells. The LAB mixture attenuated the IM and prevented blood cell decreases associated with 5-FU treatment. Mice that received 5-FU and LAB mixture decreased tumour growth and showed modulation of systemic cytokines modified by both tumour growth and 5-FU treatment. The LAB mixture by itself delayed tumour growth. Conclusions: The mixture of selected LAB was able to reduce the side-effects associated with chemotherapy without affecting its primary anti-tumour activity. Significance and Impact of the Study: This bacterial mixture could prevent the interruption of conventional oncologic therapies by reducing undesirable side-effects. In addition, this blend would provide essential nutrients (vitamins) to oncology patients. Fil: Levit, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Published: 2021

20. Recent update on lactic acid bacteria producing riboflavin and folates: application for food fortification and treatment of intestinal inflammation

Author: Romina Levit, Jean Guy LeBlanc, A. de Moreno de LeBlanc, and G. Savoy de Giori
Subjects: Mucositis, INTESTINAL INFLAMMATION, Riboflavin, Biofortification, Shelf life, Applied Microbiology and Biotechnology, RIBOFLAVIN, Antioxidants, purl.org/becyt/ford/1 [https], 03 medical and health sciences, chemistry.chemical_compound, Folic Acid, Lactobacillales, LACTIC ACID BACTERIA, Animals, Humans, Food science, purl.org/becyt/ford/1.6 [https], Fermentation in food processing, 030304 developmental biology, 0303 health sciences, biology, 030306 microbiology, Food fortification, food and beverages, Vitamins, General Medicine, Inflammatory Bowel Diseases, biology.organism_classification, CANCER, Lactic acid, chemistry, Fermentation, Food, Fortified, FOLATES, Fermented Foods, Bacteria, Biotechnology
Abstract: Lactic acid bacteria (LAB), widely used as starter cultures for the fermentation of a large variety of food, can improve the safety, shelf life, nutritional value and overall quality of the fermented products. In this regard, the selection of strains delivering health-promoting compounds is now the main objective of many researchers. Although most LAB are auxotrophic for several vitamins, it is known that certain strains have the capability to synthesize B-group vitamins. This is an important property since humans cannot synthesize most vitamins, and these could be obtained by consuming LAB fermented foods. This review discusses the use of LAB as an alternative to fortification by the chemical synthesis to increase riboflavin and folate concentrations in food. Moreover, it provides an overview of the recent applications of vitamin-producing LAB with anti-inflammatory/antioxidant activities against gastrointestinal tract inflammation. This review shows the potential uses of riboflavin and folates producing LAB for the biofortification of food, as therapeutics against intestinal pathologies and to complement anti-inflammatory/anti-neoplastic treatments. Fil: Levit, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Published: 2021

21. Probiotics and Trained Immunity

Author: Cortes-Perez, Naima G., primary, de Moreno de LeBlanc, Alejandra, additional, Gomez-Gutierrez, Jorge G., additional, LeBlanc, Jean Guy, additional, and Bermúdez-Humarán, Luis G., additional
Published: 2021
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22. Tropical fruit by-products water extracts as sources of soluble fibres and phenolic compounds with potential antioxidant, anti-inflammatory, and functional properties

Author: Raquel Bedani, Alejandra de Moreno de LeBlanc, Romina Levit, Jean Guy LeBlanc, Carolina Beres, Susana Marta Isay Saad, and Marcela Albuquerque Cavalcanti de Albuquerque
Subjects: 0301 basic medicine, Folate, Streptococcus thermophilus, Antioxidant, medicine.drug_class, medicine.medical_treatment, Medicine (miscellaneous), Orange (colour), Bacterial growth, Probiotic, Tropical fruit, Anti-inflammatory, law.invention, 03 medical and health sciences, 0404 agricultural biotechnology, Lactobacillus rhamnosus, law, medicine, TX341-641, Fruit by-products, Food science, 030109 nutrition & dietetics, Nutrition and Dietetics, biology, Nutrition. Foods and food supply, Chemistry, Anti-inflammatory effect, Dietary fibre, food and beverages, 04 agricultural and veterinary sciences, biology.organism_classification, 040401 food science, Phenolic compounds, COMPOSTOS FENÓLICOS, Food Science
Abstract: Fibre content, phenolic content, antioxidant and anti-inflammatory activities were evaluated for water extracts of fruit by-products (passion fruit, orange, acerola, and mango). The impact of these extracts on microbial growth, folate production, and adhesion ability of Streptococcus thermophilus TH-4 and Lactobacillus rhamnosus LGG was investigated. Mango water extract (MWE) presented the highest phenolic content and antioxidant activity. Orange water extract (OWE) and MWE showed the best anti-inflammatory potential by decreasing the highest nitric oxide levels. When TH-4 and LGG were grown together, folate production was only stimulated by MWE. Passion fruit water extract and OWE increased the TH-4 adhesion whereas acerola water extracts and MWE improved LGG adhesion when strains were used individually. These results showed that fruit by-product water extracts (FWE), especially from mango, presented potential beneficial biological and functional properties. These FWE could be used to develop new functional antioxidant foods and natural pharmaceutical ingredients.
Published: 2019

23. Evaluation of a Streptococcus thermophilus strain with innate anti-inflammatory properties as a vehicle for IL-10 cDNA delivery in an acute colitis model

Author: del Carmen, Silvina, Miyoshi, Anderson, Azevedo, Vasco, de Moreno de LeBlanc, Alejandra, and LeBlanc, Jean Guy
Published: 2015
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24. Maternal dietary omega-3 deficiency worsens the deleterious effects of prenatal inflammation on the gut-brain axis in the offspring across lifetime

Author: Patricia Thebault, Agnès Aubert, Philippe Langella, Boris Matrot, J. Bourel, Lionel Bretillon, A. Sere, Sophie Layé, Galadriel Brière, Quentin Leyrolle, Agnès Nadjar, Corinne Joffre, A. de Moreno de LeBlanc, Thomas Bourgeois, F. Decoeur, Gabriel Fernandes, Jean Guy LeBlanc, A. Benmamar-Badel, Jean-Marc Chatel, I Voytyuk, Raluca Uricaru, Florian Chain, Camille Amadieu, Stéphane Grégoire, Pierre Gressens, Leslie Schwendimann, Chloé Lacabanne, A R A A Quadros, Nutrition et Neurobiologie intégrée (NutriNeuro), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Maladies neurodéveloppementales et neurovasculaires (NeuroDiderot (UMR_S_1141 / U1141)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Bourgogne Franche-Comté [COMUE] (UBFC), Ctr Referencia Lactobacilos CERELA CONICET, Partenaires INRAE, Fiocruz Minas - René Rachou Research Center / Instituto René Rachou [Belo Horizonte, Brésil], Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire Bordelais de Recherche en Informatique (LaBRI), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB), The Institut National pour la Recherche Agronomique, l'Alimentation et l'Environnement (INRAE), the Bordeaux Univ, the New Aquitania Region, NeurATRIS (Paris, France), the International Laboratory OptiNutriBrain, the Foundation for Medical Research (FRM) (DEQ20170336724), the French Foundation (FDF, #00070700)., ANR-11-INBS-0011,NeurATRIS,Infrastructure de Recherche Translationnelle pour les Biothérapies en Neurosciences(2011), ANR-10-LABX-0043,BRAIN,Bordeaux Region Aquitaine Initiative for Neuroscience(2010), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), BRETILLON, LIONEL, Infrastructures - Infrastructure de Recherche Translationnelle pour les Biothérapies en Neurosciences - - NeurATRIS2011 - ANR-11-INBS-0011 - INBS - VALID, Bordeaux Region Aquitaine Initiative for Neuroscience - - BRAIN2010 - ANR-10-LABX-0043 - LABX - VALID, Développment d'une infrastructure française distribuée coordonnée - - France-BioImaging2010 - ANR-10-INBS-0004 - INBS - VALID, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, CERELA-CONICET [Tucuman, Argentina], Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Lyon (ENS Lyon), Nutrition et Neurobiologie intégrée (NutriNeur0), Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Physiopathologie, conséquences fonctionnelles et neuroprotection des atteintes du cerveau en développement, Université Paris Diderot - Paris 7 (UPD7)-IFR2-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Science du Sol, Institut National de la Recherche Agronomique (INRA), Histoire naturelle de l'Homme préhistorique (HNHP), Muséum national d'Histoire naturelle (MNHN)-Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Centro de Referencia para Lactobacilos [Tucumán] (CERELA), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Observatoire de Paris - Site de Meudon (OBSPM), Observatoire de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique
Subjects: Offspring, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Gut–brain axis, Physiology, Gut flora, Article, 03 medical and health sciences, 0302 clinical medicine, Pregnancy, Fatty Acids, Omega-3, Medicine, Animals, Humans, Pharmacology, chemistry.chemical_classification, Inflammation, Prenatal inflammation, Human studies, biology, Behavior, Animal, business.industry, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Brain, biology.organism_classification, 030227 psychiatry, Psychiatry and Mental health, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, chemistry, Prenatal Exposure Delayed Effects, Etiology, Female, Microglia, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery, Immune activation, Polyunsaturated fatty acid
Abstract: International audience; Maternal immune activation (MIA) and poor maternal nutritional habits are risk factors for the occurrence of neurodevelopmental disorders (NDD). Human studies show the deleterious impact of prenatal inflammation and low n-3 polyunsaturated fatty acid (PUFA) intake on neurodevelopment with long-lasting consequences on behavior. However, the mechanisms linking maternal nutritional status to MIA are still unclear, despite their relevance to the etiology of NDD. We demonstrate here that low maternal n-3 PUFA intake worsens MIA-induced early gut dysfunction, including modification of gut microbiota composition and higher local inflammatory reactivity. These deficits correlate with alterations of microglia-neuron crosstalk pathways and have long-lasting effects, both at transcriptional and behavioral levels. This work highlights the perinatal period as a critical time window, especially regarding the role of the gut-brain axis in neurodevelopment, elucidating the link between MIA, poor nutritional habits, and NDD. Fig. 1 EFFECT OF N-3 PUFA DEFICIENCY ON MIA-INDUCED BEHAVIORAL DEFICITS IN NEONATES AND IN ADULT OFFSPRING.: All graphs show Means ± SEM. a Experimental setup. b Average time spent by pups to achieve the Fox battery tests (negative geotaxis and righting reflex; 3 trials per day from PND4 to PND6). N = 14-19. Two-way ANOVA: MIA effect, F(1,62) = 11.67, p = 0.0011. c Average vocalization time (15-min sessions at PND7-8). N = 14-19. Kruskal-Wallis test followed by Mann-Whitney comparison; n-3 sufficient-Saline vs n-3 sufficient-LPS, **p
Published: 2021

25. Neuroprotective effects associated with immune modulation by selected lactic acid bacteria in a Parkinson's disease model

Author: Jean Guy LeBlanc, Alejandra de Moreno de LeBlanc, Daiana E. Pérez Visñuk, and Graciela Savoy de Giori
Subjects: 0301 basic medicine, Parkinson's disease, Endocrinology, Diabetes and Metabolism, 030209 endocrinology & metabolism, Inflammation, ANIMAL MODEL, Neuroprotection, law.invention, Microbiology, Mice, 03 medical and health sciences, Probiotic, chemistry.chemical_compound, 0302 clinical medicine, Animal model, INFLAMMATION, Lactobacillales, law, LACTIC ACID BACTERIA, medicine, Animals, Streptococcus thermophilus, MOTOR BEHAVIOR, purl.org/becyt/ford/3.4 [https], 030109 nutrition & dietetics, Nutrition and Dietetics, biology, Probiotics, PROBIOTIC, Parkinson Disease, Immune modulation, medicine.disease, biology.organism_classification, Lactic acid, Neuroprotective Agents, chemistry, purl.org/becyt/ford/3 [https], medicine.symptom, PARKINSON'S DISEASE, Bacteria
Abstract: Objectives: Parkinson's disease (PD) is a neurodegenerative process that affects the motor function and involves an inflammatory response and B vitamin deficiencies. The aim of this study was to evaluate the effect of B-group vitamin-producing and immunomodulatory lactic acid bacteria (LAB) in a murine model of PD. Methods: The effect of Lactobacillus plantarum CRL 2130 (a riboflavin producer), Streptococcus thermophilus CRL 807 (an immunomodulatory strain), and Streptococcus thermophilus CRL 808 (a folate producer) were evaluated individually and as a mixture in mice injected with 1-methyl-4-fenil-1,2,3,6-tetrahidropiridina. Motor capacity, tyrosine hydrolase in the brain, and cytokine concentrations in serum and brain tissues were evaluated in 1-methyl-4-fenil-1,2,3,6-tetrahidropiridina-treated mice after bacterial supplementation. Results: The mice receiving the selected LAB showed significantly improved motor skills compared with those that did not receive bacterial supplementation. When given the mixture of all 3 strains together, the animals had higher brain tyrosine hydrolase-positive cell counts, decreased inflammatory cytokines interleukin 6 and tumor necrosis factor alpha in serum, and increased antiinflammatory cytokine interleukin 10 in serum and brain tissues compared with animals that did not receive LAB supplementation. Conclusions: The results showed the potential of a selected LAB mixture to improve motor behavior and neuroinflammation in PD. This probiotic mixture could be used as an adjunct treatment in the control of PD. Fil: Pérez Visñuk, Daiana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Published: 2020

26. Análisis de las propiedades probióticas de cepas sobreproductoras de riboflavina derivadas de bacterias aisladas de chicha Argentina

Author: Mohedano Bonillo, Mari Luz, Hernández-Alcántara, Annel M., Pardo, S., Requena, Teresa, De Moreno de LeBlanc, A., Leblanc, Jean Guy, Aznar, Rosa, and López, Paloma
Abstract: Trabajo presentado al XI Workshop Sociedad Española de Microbiota, Probióticos y Prebióticos, celebrado en Granada del 12 al 14 de febrero de 2020., [Introducción/Objetivos]: Algunas bacterias ácido lácticas (BAL) producen riboflavina, vitamina B2 esencial para los seres humanos. Por ello pueden ser utilizadas para la elaboración de alimentos funcionales. En este trabajo se pretendió caracterizar in vitro e in vivo tres cepas de Lactobacillus plantarum sobreproductoras de riboflavina, derivadas de BAL aisladas de chicha argentina., [Metodología]: Las BAL fueron marcadas por transferencia del plásmido pRCR12, que codifica la proteína fluorescente mCherry. Los niveles de riboflavina y de mCherry se determinaron fluorimetricamente. Se analizó la adhesión de las BAL a células epiteliales Caco-2. La formación de biopelículas se evaluó por tinción con cristal violeta y por la fluorescencia de mCherry, que también se utilizó para analizar la autoagregación de las BAL. Se determinó la supervivencia de las BAL bajo estrés gastrointestinal en presencia de una matriz alimentaria comercial (Incaparina). La supervivencia in vivo de las BAL libres o en conjunción con Incaparina se evaluó en un modelo murino con ratones convencionales BALB/c., [Resultados]: El marcaje de las BAL con pRCR12 no afectó de forma significativa su crecimiento o su producción de riboflavina. Las tres cepas mostraron niveles semejantes de adhesión a los enterocitos (4-6%) y similares a los del probiótico Lactobacillus rhamnosus LGG (4%). Las tres cepas formaron biopelículas consistentes. M9MG6B2 y M5MA1B2 mostraron una agregación superior (30-40%) a la de M9MM1B2 (18%). Se detectó in vitro un efecto protector de la Incaparina y una resistencia satisfactoria de las tres cepas al estrés gastrointestinal. Únicamente M9MM1B2 mostró cierta susceptibilidad a pH 2,0 (supervivencia del 63%). La administración de las BAL solas o junto con Incaparina a roedores, no tuvo efecto adverso sobre la salud, el crecimiento y/o el bienestar de los roedores., [Conclusiones]: Los resultados obtenidos indican que las tres BAL estudiadasposeen características probióticas de interés para el desarrollo de alimentos funcionales.
Published: 2020

27. Effect of riboflavin-producing bacteria against chemically induced colitis in mice

Author: Jean Guy LeBlanc, G. Savoy de Giori, Romina Levit, and A. de Moreno de LeBlanc
Subjects: 0301 basic medicine, Vitamin, Streptococcus thermophilus, Otras Ciencias Biológicas, Riboflavin, Probiotic, Applied Microbiology and Biotechnology, law.invention, Microbiology, Proinflammatory cytokine, Ciencias Biológicas, purl.org/becyt/ford/1 [https], Feces, Mice, Lactic Acid Bacteria, 03 medical and health sciences, chemistry.chemical_compound, law, Lactobacillus, medicine, Animals, Humans, Colitis, purl.org/becyt/ford/1.6 [https], Mice, Inbred BALB C, biology, Probiotics, food and beverages, General Medicine, biology.organism_classification, medicine.disease, Interleukin-10, Lactic acid, Intestines, 030104 developmental biology, Trinitrobenzenesulfonic Acid, chemistry, Female, CIENCIAS NATURALES Y EXACTAS, Anti-Inflammatory Effect, Biotechnology
Abstract: Aim: To assess the anti-inflammatory effect associated with individual probiotic suspensions of riboflavin-producing lactic acid bacteria (LAB) in a colitis murine model. Methods and Results: Mice intrarectally inoculated with trinitrobenzene sulfonic acid (TNBS) were orally administered with individual suspensions of riboflavin-producing strains: Lactobacillus (Lact.) plantarum CRL2130, Lact. paracasei CRL76, Lact. bulgaricus CRL871 and Streptococcus thermophilus CRL803; and a nonriboflavin-producing strain or commercial riboflavin. The extent of colonic damage and inflammation and microbial translocation to liver were evaluated. iNOs enzyme was analysed in the intestinal tissues and cytokine concentrations in the intestinal fluids. Animals given either one of the four riboflavin-producing strains showed lower macroscopic and histologic damage scores, lower microbial translocation to liver, significant decreases of iNOs+ cells in their large intestines and decreased proinflammatory cytokines, compared with mice without treatment. The administration of pure riboflavin showed similar benefits. Lact. paracasei CRL76 accompanied its anti-inflammatory effect with increased IL-10 levels demonstrating other beneficial properties in addition to the vitamin production. Conclusion: Administration of riboflavin-producing strains prevented the intestinal damage induced by TNBS in mice. Significance and Impact of the Study: Riboflavin-producing phenotype in LAB represents a potent tool to select them for preventing/treating IBD. Fil: Levit, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Published: 2017

28. Role of yoghurt in the prevention of colon cancer

Author: Perdigón, G, de Moreno de LeBlanc, A, Valdez, J, and Rachid, M
Published: 2002
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29. Probiotics and Trained Immunity

Author: Luis G. Bermúdez-Humarán, Jorge G. Gomez-Gutierrez, Naima G. Cortes-Perez, Alejandra de Moreno de LeBlanc, Jean Guy LeBlanc, Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centro de Referencia para Lactobacilos [Tucumán] (CERELA), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Department of Child Health, School of Medicine, University of Missouri, Columbia, MO 65201, USA, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas, project PIP 0697, ANPCyT (Agencia Nacional de Promoción Científica y Tecnológica, projects PICT2016-0301and PICT2019-0942), Argentina, and NCI (National Cancer Institute, project R21CA210202, J.G.G.-G.), USA
Subjects: HUMAN HEALTH, [SDV]Life Sciences [q-bio], animal diseases, education, chemical and pharmacologic phenomena, Context (language use), Review, Biology, Health benefits, human health, Microbiology, Biochemistry, immune response, Epigenesis, Genetic, trained immunity, 03 medical and health sciences, Human health, 0302 clinical medicine, Immune system, Immunity, IMMUNE RESPONSE, Humans, Molecular Biology, purl.org/becyt/ford/2.11 [https], 030304 developmental biology, 0303 health sciences, Innate immune system, TRAINED IMMUNITY, biochemical phenomena, metabolism, and nutrition, PROBIOTICS, Immunity, Innate, QR1-502, 3. Good health, purl.org/becyt/ford/2 [https], probiotics, bacteria, Neuroscience, 030215 immunology
Abstract: The characteristics of innate immunity have recently been investigated in depth in several research articles, and original findings suggest that innate immunity also has a memory capacity, which has been named “trained immunity”. This notion has revolutionized our knowledge of the innate immune response. Thus, stimulation of trained immunity represents a therapeutic alternative that is worth exploring. In this context, probiotics, live microorganisms which when administered in adequate amounts confer a health benefit on the host, represent attractive candidates for the stimulation of trained immunity; however, although numerous studies have documented the beneficial proprieties of these microorganisms, their mechanisms of action are not yet fully understood. In this review, we propose to explore the putative connection between probiotics and stimulation of trained immunity. Fil: Cortes Peres, Naima G.. Institut National de la Recherche Agronomique; Francia Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Gomez Gutierrez, Jorge G.. University of Missouri; Estados Unidos Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Bermúdez Humarán, Luis G.. Institut National de la Recherche Agronomique; Francia
Published: 2021

30. Study of cytokines involved in the prevention of a murine experimental breast cancer by kefir

Author: de Moreno de LeBlanc, A., Matar, C., Farnworth, E., and Perdigon, G.
Published: 2006
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31. The Ability of Riboflavin-Overproducing Lactiplantibacillus plantarum Strains to Survive Under Gastrointestinal Conditions

Author: Hernández-Alcántara, Annel M., primary, Pardo, Sandra, additional, Mohedano, Mari Luz, additional, Vignolo, Graciela M., additional, de Moreno de LeBlanc, Alejandra, additional, LeBlanc, Jean Guy, additional, Aznar, Rosa, additional, and López, Paloma, additional
Published: 2020
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32. Maternal dietary omega-3 deficiency worsens the deleterious effects of prenatal inflammation on the gut-brain axis in the offspring across lifetime

Author: Leyrolle, Q., primary, Decoeur, F., additional, Briere, G., additional, Amadieu, C., additional, Quadros, A. R. A. A., additional, Voytyuk, I., additional, Lacabanne, C., additional, Benmamar-Badel, A., additional, Bourel, J., additional, Aubert, A., additional, Sere, A., additional, Chain, F., additional, Schwendimann, L., additional, Matrot, B., additional, Bourgeois, T., additional, Grégoire, S., additional, Leblanc, J. G., additional, De Moreno De Leblanc, A., additional, Langella, P., additional, Fernandes, G. R., additional, Bretillon, L., additional, Joffre, C., additional, Uricaru, R., additional, Thebault, P., additional, Gressens, P., additional, Chatel, J. M., additional, Layé, S., additional, and Nadjar, A., additional
Published: 2020
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33. Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice

Author: de Moreno de LeBlanc, Alejandra, LeBlanc, Jean Guy, Perdigón, Gabriela, Miyoshi, Anderson, Langella, Philippe, Azevedo, Vasco, and Sesma, Fernando
Published: 2008
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34. Oral administration of milk fermented by Lactobacillus casei CRL431 was able to decrease metastasis from breast cancer in a murine model by modulating immune response locally in the lungs

Author: Alejandra de Moreno de LeBlanc, Virginia Emilce Mendez Utz, and Gabriela Perdigón
Subjects: 0301 basic medicine, Lactobacillus casei, Otras Ciencias Biológicas, Medicine (miscellaneous), Probiotic, law.invention, Metastasis, Ciencias Biológicas, purl.org/becyt/ford/1 [https], 03 medical and health sciences, 0404 agricultural biotechnology, Breast cancer, Immune system, Oral administration, law, BREAST CANCER, IMMUNE RESPONSE, medicine, TX341-641, Immune response, purl.org/becyt/ford/1.6 [https], 030109 nutrition & dietetics, Nutrition and Dietetics, biology, business.industry, Nutrition. Foods and food supply, PROBIOTIC, 04 agricultural and veterinary sciences, medicine.disease, biology.organism_classification, 040401 food science, In vitro, Lung metastasis, Cancer research, LUNG METASTASIS, business, CIENCIAS NATURALES Y EXACTAS, CD8, Food Science
Abstract: The host immune system is an important factor to combat metastasis. In this work, it was evaluated the immunomodulation exerted by milk fermented with the probiotic strain Lactobacillus casei CRL431 (PFM) when was administered to mice in the metastatic stage of breast cancer. Mice received PFM were compared to animals given milk. PFM administration diminished metastasis in the lungs and increased the survival of the animals. Systemically, PFM administration decreased pro-inflammatory cytokines and locally in the lungs (metastatic organs) decreased F4/80+ cells, principally IL-10/F4/80+ cells. The highest percentages for CD4+ and CD4+CD8+ cells were observed in PFM group. Finally, immune cells from lungs of mice given PFM showed lower production of most cytokines evaluated when they were re-stimulated in vitro with tumour cells. The results obtained suggest that modulation of immune cells in the lungs by PFM could be a strategy to fight against tumour cells in the metastatic sites. Fil: Méndez Utz, Virginia Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Perdigon, Gabriela del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Published: 2019

35. Development of a potential probiotic yoghurt using selected anti-inflammatory lactic acid bacteria for prevention of colitis and carcinogenesis in mice

Author: Jean Guy LeBlanc, A. de Moreno de LeBlanc, and S. del Carmen
Subjects: Carcinogenesis, Colorectal cancer, medicine.disease_cause, Applied Microbiology and Biotechnology, law.invention, Mice, STREPTOCOCCUS THERMOPHILUS, Probiotic, chemistry.chemical_compound, 0302 clinical medicine, LACTIC ACID BACTERIA, law, Mice, Inbred BALB C, CARCINOGENESIS, food and beverages, General Medicine, Colitis, Yogurt, Lactic acid, Intestines, Lactobacillaceae, 030220 oncology & carcinogenesis, Colonic Neoplasms, Female, 030211 gastroenterology & hepatology, medicine.symptom, COLITIS, Biotechnology, CIENCIAS MÉDICAS Y DE LA SALUD, medicine.drug_class, Inflammation, Biology, Anti-inflammatory, Biotecnología de la Salud, Microbiology, 03 medical and health sciences, Immune system, medicine, Animals, Humans, Lactic Acid, Probiotics, LACTOBACILLUS BULGARICUS, medicine.disease, chemistry, Fermentation, Immunology, Otras Biotecnologías de la Salud
Abstract: Aims: To evaluate the beneficial properties of a potentially probiotic yoghurt obtained by the fermentation of two selected anti-inflammatory bacterial strains using in vivo mouse models of intestinal inflammation and colon carcinogenesis. Methods and Results: Yoghurt was administered to mice suffering chemically induced intestinal inflammation or colon carcinogenesis. It was shown that this novel yoghurt was able to prevent local inflammation in the intestines of mice through a regulation of the immune response, prevent macroscopic and histological damages, and prevent colon carcinogenesis through an anti-inflammatory response. Conclusions: The developed yoghurt showed in vivo anti-inflammatory properties by modulation of the host immune response for the prevention of colon inflammation and carcinogenesis. Significance and Impact of the Study: This new yoghurt could thus be considered a probiotic food and be useful as a complement to current treatment protocols for inflammatory bowel diseases and colon cancer, a first since there are no current functional foods specifically oriented for these patients. Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Published: 2016

36. Retraction Note to: Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer

Author: Jean Guy LeBlanc, Daniel Lozano-Ojalvo, Florian Chain, Alejandra de Moreno de LeBlanc, Luis G. Bermúdez-Humarán, Naima G. Cortes-Perez, Marion Lenoir, Philippe Langella, Silvina del Carmen, and Diego Muñoz-Provencio
Subjects: medicine.medical_specialty, Lactobacillus casei, biology, Colorectal cancer, business.industry, T cell, Gastroenterology, Hepatology, medicine.disease, biology.organism_classification, Colorectal surgery, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, Surgical oncology, 030220 oncology & carcinogenesis, Internal medicine, medicine, 030211 gastroenterology & hepatology, business, Abdominal surgery
Abstract: This article has been retracted. Please see the retraction notice for more detail: https://doi.org/10.1007/s00535-015-1158-9 .
Published: 2020

37. Evaluation of the bioavailability and intestinal effects of milk fermented by folate producing lactic acid bacteria in a depletion/repletion mice model

Author: Katia Gianni, Bernadette Dora Gombossy de Melo Franco, Ana Clara C. Cucick, Alejandra de Moreno de LeBlanc, Jean Guy LeBlanc, and Svetoslav Dimitrov Todorov
Subjects: 0301 basic medicine, Folate, Streptococcus thermophilus, Bioavailability, Otras Ingenierías y Tecnologías, BIOAVAILABILITY, Medicine (miscellaneous), INGENIERÍAS Y TECNOLOGÍAS, Hematocrit, Alimentos y Bebidas, VITAMIN DEFICIENCY, 03 medical and health sciences, chemistry.chemical_compound, 0404 agricultural biotechnology, Intestinal mucosa, LACTIC ACID BACTERIA, Lactic acid bacteria, medicine, Vitamin deficiency, TX341-641, Food science, purl.org/becyt/ford/2.11 [https], 030109 nutrition & dietetics, Nutrition and Dietetics, biology, medicine.diagnostic_test, Nutrition. Foods and food supply, food and beverages, Fermented milk, 04 agricultural and veterinary sciences, FERMENTED MILK, biology.organism_classification, 040401 food science, Lactic acid, purl.org/becyt/ford/2 [https], chemistry, Fermentation, CAMUNDONGOS, Lactobacillus plantarum, Bacteria, FOLATE, Food Science
Abstract: This study aimed to evaluate the bioavailability and effects on the intestinal mucosa of a bioenriched fermented milk (BFM) prepared with folate-producing lactic acid bacteria, using a depletion/repletion BALB/c mice model. Five Streptococcus thermophilus strains were combined with Lactobacillus plantarum 16cv and the combination that resulted in the best folate production was used for preparation of BFM. The folate content in BFM, prepared under controlled conditions (pH 6.0, 42 °C, 70 rpm, 24 h), was 321.1 ± 14.1 ng/mL. Results for mice fed with BFM were compared to those fed with other diets. The BFM group presented an increase in hemoglobin, hematocrit and red blood cells, and the villi height/crypt depth ratio was similar to that of animals receiving milk supplemented with commercial folic acid. The strains were sensitive to most tested antibiotics and lacked virulence genes, indicating that consumption of BFM may be a promising alternative to increase intake of folate. Fil: Cucick, Ana Clara C.. Universidade de Sao Paulo; Brasil Fil: Gianni de Carvalho, Katia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Todorov, Svetoslav D.. Universidade de Sao Paulo; Brasil Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Franco, Bernadette D. G. M.. Universidade de Sao Paulo; Brasil
Published: 2020

38. Effect of Yoghurt on the Cytokine Profile using a Murine Model of Intestinal Inflammation

Author: Silvina Chaves, A. de Moreno de LeBlanc, and Gabriela Perdigón
Subjects: 0301 basic medicine, CIENCIAS MÉDICAS Y DE LA SALUD, Cytokine profile, Immunology, Inmunología, lcsh:Medicine, Inflammation, Disease, Inflammatory bowel disease, 03 medical and health sciences, 0302 clinical medicine, Intestinal inflammation, medicine, Immunology and Allergy, business.industry, lcsh:R, food and beverages, purl.org/becyt/ford/3.1 [https], medicine.disease, Ulcerative colitis, digestive system diseases, Medicina Básica, 030104 developmental biology, Murine model, Yoghurt, 030220 oncology & carcinogenesis, Intestinal Microbiota, Etiology, Cytokines, purl.org/becyt/ford/3 [https], medicine.symptom, business
Abstract: Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, are important problems in industrialized countries. The complete aetiology of both diseases is still unknown but likely involves genetic, environmental and immunological factors. The aim of this work is to study the anti-inflammatory mechanisms reported for yoghurt in a colon cancer model in order to evaluate its usefulness in the treatment of intestinal inflammation such as Crohn's disease. A trinitrobenzenesulfonic acid (TNBS)-induced colitis model was used. The influence of yoghurt feeding was studied before and after TNBS inoculation. The effect on the intestinal microbiota and on the host immune response was evaluated. IgA-producing cells and cells positive for specific cytokines (IL-12, IL-17, IFNγ and IL-10) were analyzed. Yoghurt administration diminished the severity of inflammation in the TNBS-inoculated mice. This effect occurred mainly through IL-10, which was increased in the intestinal tissues throughout the study, and by the decrease observed in IL-17 and IL-12 levels. In addition to this immunomodulatory capacity, another mechanism by which yoghurt could exert the anti-inflammatory activity observed in our model would be through beneficial changes in the intestinal microbiota (increases in the bifidobacteria and lactobacilli populations). These changes in the intestinal microbiota could also be considered one of the causes of the intestinal inflammation reduction. These results show that yoghurt administration modulated the immune response, inducing down regulation of the inflammatory cytokines produced by the immune cells involved in the inflammatory process. The protective effect. Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Chaves, Analia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Published: 2017

39. Evaluation of the biosafety of recombinant lactic acid bacteria designed to prevent and to treat colitis

Author: Philippe Langella, Luis G. Bermúdez-Humarán, Alejandra de Moreno de LeBlanc, Silvina del Carmen, Vasco Azevedo, Jean-Marc Chatel, Jean Guy LeBlanc, de Moreno de LeBlanc, Alejandra, Del Carmen, Silvina, Centro de Referencia para Lactobacilos [Tucumán] (CERELA), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, and Federal University of Minas Gerais
Subjects: Animal Experimentation, 0301 basic medicine, Microbiology (medical), Streptococcus thermophilus, Drug-Related Side Effects and Adverse Reactions, [SDV]Life Sciences [q-bio], 030106 microbiology, Biology, Inflammatory bowel diseases, Microbiology, law.invention, 03 medical and health sciences, chemistry.chemical_compound, LACTIC ACID BACTERIA, law, genetically modified microorganisms, medicine, Animals, Colitis, bacteria, Mice, Inbred BALB C, Gastrointestinal tract, lactic acid, risk assessment, GENETICALLY MODIFIED MICROORGANISMS, Probiotics, Lactococcus lactis, food and beverages, General Medicine, medicine.disease, biology.organism_classification, 3. Good health, Genetically modified organism, Lactic acid, Biological Therapy, 030104 developmental biology, chemistry, SAFETY, Immunology, Recombinant DNA, Female, Bacteria, COLITIS
Abstract: Inflammatory bowel diseases (IBDs) affect the gastrointestinal tract and are characterized by recurrent inflammation that requires lifelong therapies. Probiotics such as lactic acid bacteria (LAB) have been proposed to complement current treatment protocols for these patients; however, their characteristics are strain dependent. In this regard, certain novel characteristics are only possible through the genetic modification of these beneficial micro-organisms. Different delivery systems, such as protein delivery of anti-oxidant enzymes and anti-inflammatory cytokines, have been shown to be effective in preventing and treating IBD in animal models. In this study, the safety of the recombinant LAB (recLAB) Streptococcus thermophilus CRL807: CAT, S. thermophilus CRL807: SOD, Lactococcus lactis NCDO2118 pXILCYT: IL-10, L. lactis MG1363 pValac: IL-10 and L. lactis MG1363 pGroESL: IL-10 with proven beneficial effects was compared to their progenitor strains S. thermophilus CRL807, L. lactis NCDO2118 or L. lactis MG1363. The prolonged administration of these genetically modified strains showed that they were just as safe as the native strains from which they derive, as demonstrated by normal animal growth and relative organ weights, absence of microbial translocation from the gastrointestinal tract, normal blood parameters and intestinal histology. The results show the potential use of these recLAB in future therapeutic formulations; however, the use of modern bio-containment systems is required for the future acceptance of these recLAB by the medical community and patients with IBD. Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Chatel, Jean Marc. INRA and AgroParisTech; Francia Fil: Azevedo, Vasco. Universidade Federal do Minas Gerais; Brasil Fil: Langella, Philippe. INRA and AgroParisTech; Francia Fil: Bermudez Humaran, Luis. INRA and AgroParisTech; Francia Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Published: 2016

40. Effect of a probiotic fermented milk on the thymus in Balb/c mice under non-severe protein–energy malnutrition

Author: Ivanna Novotny Núñez, Alejandra de Moreno de LeBlanc, Carolina Maldonado Galdeano, Ricardo Weill, Gabriela Perdigón, and Esteban Carmuega
Subjects: Male, CIENCIAS MÉDICAS Y DE LA SALUD, Protein–energy malnutrition, Ciencias de la Salud, Medicine (miscellaneous), Physiology, Apoptosis, Thymus Gland, Kangai-1 Protein, Protein-Energy Malnutrition, Severity of Illness Index, BALB/c, law.invention, Mice, Probiotic, Immune system, law, Animals, Medicine, THYMUS, Mice, Inbred BALB C, Thymocytes, Nutrition and Dietetics, biology, business.industry, Probiotics, Histology, NON-SEVERE-PROTEIN ENERGY MALNUTRITION, MOUSE MODEL, FERMENTED MILK, biology.organism_classification, medicine.disease, PROBIOTICS, Otras Ciencias de la Salud, Malnutrition, Milk, CD4 Antigens, Dietary Supplements, Fermentation, Immunology, Food Microbiology, Cytokines, Female, business
Abstract: Protein–energy malnutrition (PEM) causes a significant impairment of the immune system, the thymus being one of the most affected organs. It has been demonstrated that the administration of probiotic fermented milk (PFM) recovered the intestinal barrier, histological alterations and mucosal and systemic immune functions in a non-severe malnutrition model using BALB/c mice. The aim of the present study was to evaluate, in the same model of malnutrition, the effect of a PFM added to a re-nutrition diet on the recovery of the thymus, analysing histological and functional alterations caused by malnutrition. Mice were undernourished and divided into three groups according to the dietary supplement received during re-nutrition: milk, PFM or its bacterial-free supernatant (BFS). They were compared with well-nourished and malnourished mice. PFM was the most effective re-nutrition supplement to improve the histology of the thymus, decreasing cellular apoptosis in this organ and recovering the percentage of CD4þ/CD82 single-positive thymocytes. Immature doublepositive thymocytes were increased in the malnourished control (MC). The production of different cytokines in the thymus was increased in mice given PFM, compared with the mice that received other dietary supplements and MC. Mice given the BFS presented an improvement in the thymus similar to those that received milk. We demonstrated the importance of the whole PFM supplementation on the histological and functional recovery of the thymus in a non-severe PEM model. Fil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina; Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina; Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina; Fil: Carmuega, Esteban. Centro de Estudios en Nutrición Infantil; Argentina; Fil: Weill, Ricardo. DANONE. Departamento de investigación y Desarrollo; Argentina; Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina; Fil: Perdigón, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina; Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Published: 2013

41. Mechanisms Involved in the Anti-Inflammatory Properties of Native and Genetically Engineered Lactic Acid Bacteria

Author: Daniela Santos Pontes, Jean Guy LeBlanc, Anderson Miyoshi, Alejandra de Moreno de LeBlanc, Meritxell Zurita Turk, Fernanda Alvarenga Lima, Vasco Azevedo, and Silvina del Carmen
Subjects: Antioxidant, biology, medicine.drug_class, Genetically engineered, medicine.medical_treatment, General Medicine, biology.organism_classification, Anti-inflammatory, Lactic acid, Superoxide dismutase, chemistry.chemical_compound, Interleukin 10, Biochemistry, chemistry, Catalase, medicine, biology.protein, Bacteria
Published: 2012

42. Soyamilk fermented with riboflavin-producing Lactobacillus plantarum CRL 2130 reverts and prevents ariboflavinosis in murine models

Author: G. Savoy de Giori, Jonathan Emiliano Laiño, A. de Moreno de LeBlanc, M. Juarez del Valle, and Jean Guy LeBlanc
Subjects: 0301 basic medicine, Riboflavin, Otras Ciencias Biológicas, Medicine (miscellaneous), Biology, RIBOFLAVIN, VITAMIN DEFICIENCY, Ciencias Biológicas, Mice, 03 medical and health sciences, Riboflavin Deficiency, LACTIC ACID BACTERIA, Animals, heterocyclic compounds, Food science, BIO-ENRICHED FOODS, SOYAMILK, Nutritional deficiency, Mice, Inbred BALB C, Nutrition and Dietetics, Mandatory fortification, Prevention model, digestive, oral, and skin physiology, food and beverages, biology.organism_classification, Diet, Soy Milk, Riboflavin deficiency, 030104 developmental biology, Fermentation, Female, Ariboflavinosis, Lactobacillus plantarum, CIENCIAS NATURALES Y EXACTAS
Abstract: It has been previously shown that Lactobacillus plantarum CRL 2130 is able to produce riboflavin in soyamilk. The aim of the present study was to evaluate the efficiency of this riboflavin-bio-enriched soyamilk to revert and/or prevent the nutritional deficiency of riboflavin using different animal models. When used to supplement the diets of previously depleted animals, it was shown that the growth, riboflavin status and morphology of the small intestines reverted to normal parameters and were similar to animals supplemented with commercial riboflavin. In the prevention model, the same tendency was observed, where animals that received soyamilk fermented with L. plantarum CRL 2130 did not show signs of riboflavin deficiency. This new bio-fortified soya-based product could be used as part of normal diets to provide a more natural alternative to mandatory fortification with riboflavin for the prevention of its deficiency. Fil: Juarez del Valle, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Laiño, Jonathan Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Published: 2016

43. Potential Application of Probiotics in the Prevention and Treatment of Inflammatory Bowel Diseases

Author: Silvina del Carmen, Alejandra de Moreno de LeBlanc, Clarissa Santos Rocha, Jean Guy LeBlanc, Vasco Azevedo, and Anderson Miyoshi
Subjects: Antioxidant, Otras Ciencias Biológicas, medicine.medical_treatment, Biology, Gut flora, medicine.disease_cause, Inflammatory bowel disease, law.invention, purl.org/becyt/ford/1 [https], Ciencias Biológicas, Superoxide dismutase, Probiotic, Immune system, LACTIC ACID BACTERIA, law, ANTIOXIDANT, medicine, purl.org/becyt/ford/1.6 [https], INFLAMMATORY BOWEL DISEASE, Inflammatory Bowel Diseases, biology.organism_classification, medicine.disease, PROBIOTICS, Immunology, biology.protein, CIENCIAS NATURALES Y EXACTAS, Oxidative stress
Abstract: Lactic acid bacteria (LAB) represent a heterogeneous group of microorganisms that are naturally present in many foods and possess a wide range of therapeutic properties. The aim of this paper is to present an overview of the current expanding knowledge of the mechanisms by which LAB and other probiotic microorganisms participate in the prevention and treatment of inflammatory bowel diseases. These include changes in the gut microbiota, stimulation of the host immune responses, and reduction of the oxidative stress due to their antioxidant properties. A brief overview of the uses of genetically engineered LAB that produce either antioxidant enzymes (such as catalase and superoxide dismutase) or anti-inflammatory cytokines (such as IL-10) will also be discussed. This paper will show that probiotics should be considered in treatment protocols of IBD since they provide many beneficial effects and can enhance the effectiveness of traditional used medicines. Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; Brasil Fil: Santos Rocha, Clarissa. Universidade Federal de Minas Gerais; Brasil Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Published: 2011

44. Use of superoxide dismutase and catalase producing lactic acid bacteria in TNBS induced Crohn's disease in mice

Author: Alejandra de Moreno de LeBlanc, Anderson Miyoshi, Philippe Langella, Fernando Sesma, Luis G. Bermúdez-Humarán, Laurie Watterlot, Silvina del Carmen, Jean Guy LeBlanc, Vasco Azevedo, Gabriela Perdigón, Centro de Referencia para Lactobacilos [Tucumán] (CERELA), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Inst Microbiol, Federal University of Minas Gerais, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Inst Microbiol, Fac Bioquim Quim & Farm, Catedra Inmunol, Univ Nacl Tucuman, Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT), Consejo de Investigaciones de la Universidad Nacional de Tucuman (CIUNT), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), and Centro Argentino Brasileno de Biotecnologia
Subjects: LACTOBACILLUS-CASEI, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Antioxidant, medicine.medical_treatment, medicine.disease_cause, Applied Microbiology and Biotechnology, Mice, chemistry.chemical_compound, Crohn Disease, Lactic acid bacteria, PROTECTION, OXIDATIVE STRESS, 2. Zero hunger, chemistry.chemical_classification, Mice, Inbred BALB C, INDUCED COLITIS, 0303 health sciences, Histocytochemistry, General Medicine, Catalase, CANCER, 3. Good health, Lactic acid, Lacticaseibacillus casei, Liver, Cytokines, Female, Genetic Engineering, Biotechnology, Lactobacillus casei, INTESTINAL INFLAMMATION, Colon, Bioengineering, Superoxide dismutase, Biology, Microbiology, 03 medical and health sciences, medicine, Animals, Intestine, Large, 030304 developmental biology, Inflammation, Reactive oxygen species, 030306 microbiology, Probiotics, Body Weight, biology.organism_classification, MODEL, Disease Models, Animal, Enzyme, Trinitrobenzenesulfonic Acid, chemistry, biology.protein, Oxidative stress, INFLAMMATORY-BOWEL-DISEASE
Abstract: Reactive oxygen species are involved in various aspects of intestinal inflammation and tumor development. Decreasing their levels using antioxidant enzymes, such as catalase (CAT) or superoxide dismutase (SOD) could therefore be useful in the prevention of certain diseases. Lactic acid bacteria (LAB) are ideal candidates to deliver these enzymes in the gut. In this study, the anti-inflammatory effects of CAT or SOD producing Lwere evaluated using a trinitrobenzenesulfonic acid (TNBS) induced Crohn's disease murine model. Engineered Lactobacillus casei BL23 strains producing either CAT or SOD, or the native strain were given to mice before and after intrarectal administration of TNBS. Animal survival, live weight, intestinal morphology and histology, enzymatic activities, microbial translocation to the liver and cytokines released in the intestinal fluid were evaluated. The mice that received CAT or SOD-producing Lshowed a faster recovery of initial weight loss, increased enzymatic activities in the gut and lesser extent of intestinal inflammation compared to animals that received the wild-type strain or those that did not receive bacterial supplementation. Our findings suggest that genetically engineered Lthat produce antioxidant enzymes could be used to prevent or decrease the severity of certain intestinal pathologies. (C) 2010 Elsevier B.V. All rights reserved.
Published: 2011

45. The application of probiotics in cancer

Author: Alejandra de Moreno de LeBlanc, Gabriela Perdigón, and Chantal Matar
Subjects: CIENCIAS MÉDICAS Y DE LA SALUD, Colorectal cancer, Inmunología, Medicine (miscellaneous), Breast Neoplasms, COLON CANCER, medicine.disease_cause, law.invention, Mice, Probiotic, Lymphocytes, Tumor-Infiltrating, Immune system, Breast cancer, BREAST CANCER, law, INTESTINE, Animals, Humans, Medicine, Carcinogen, Nutrition and Dietetics, biology, business.industry, Probiotics, food and beverages, Cancer, biology.organism_classification, medicine.disease, PROBIOTICS, Disease Models, Animal, Medicina Básica, Colonic Neoplasms, Immunology, Cytokines, business, Carcinogenesis, Bacteria
Abstract: Lactic acid bacteria (LAB) are present in many foods such as yoghurt and are frequently used as probiotics to favour some biological functions in the host. Many investigators have evaluated the therapeutic effects of yoghurt and LAB commonly used in yoghurt production against diseases such as cancer, infection, and gastrointestinal disorders. The increase of immune cell activity in the prevention of cancer by LAB consumption has also been described. Another possible explanation for the preventive effect of probiotics on carcinogenesis is their effect on other bacteria in the intestine. Probiotics may suppress the growth of bacteria that convert procarcinogens into carcinogens, thereby reducing the amount of carcinogens in the intestine. The present review is focused on two types of cancer in which milk fermented by LAB may show a beneficial effect: colon cancer and breast cancer. Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Matar, Chantal. Université de Moncton; Francia Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Published: 2007

46. Study of Immune Cells Involved in the Antitumor Effect of Kefir in a Murine Breast Cancer Model

Author: A. de Moreno de LeBlanc, Chantal Matar, Gabriela Perdigón, and Edward R. Farnworth
Subjects: CD4-Positive T-Lymphocytes, medicine.medical_specialty, CIENCIAS MÉDICAS Y DE LA SALUD, KEFIR, Cultured Milk Products, Mammary gland, Cell, Inmunología, Antineoplastic Agents, Apoptosis, Breast Neoplasms, CD8-Positive T-Lymphocytes, Biology, Mice, Mammary Glands, Animal, Breast cancer, Immune system, BREAST CANCER, Cell Line, Tumor, IMMUNE RESPONSE, Internal medicine, Genetics, medicine, Animals, Mice, Inbred BALB C, Kefir, Mammary Neoplasms, Experimental, medicine.disease, Immunoglobulin A, Disease Models, Animal, Medicina Básica, medicine.anatomical_structure, Endocrinology, Proto-Oncogene Proteins c-bcl-2, Cell culture, Dietary Supplements, Cancer research, Female, Animal Science and Zoology, CD8, Food Science
Abstract: Administration of kefir and a kefir cell-free fraction (KF) to mice injected with breast tumor cells produced, locally in the mammary gland, different profiles of cells secreting cytokines. Here, the immune cell populations in mammary glands affected by the cyclic consumption of kefir or KF for 2 or 7 d were evaluated using a breast tumor model. Apoptosis was also assayed as another mechanism involved in tumor growth delay. The rate development of tumor cells, IgA(+) cells, and CD4+ and CD8+ T lymphocytes was monitored in mammary gland tissues. The number of Bcl-2(+) cells in the mammary gland was compared with the apoptosis observed in the tumor. Two-day cyclical administration of both products delayed tumor growth and increased the number of IgA(+) cells in the mammary gland. Changes in the balance between CD4+ and CD8+ cells in the mammary gland were observed in mice from the group fed KF cyclically for 2 d, such that the number of CD4+ cells increased when the number of CD8+ cells remained constant. Mice that received 2-d cyclic administration of KF showed significant increases in the number of apoptotic cells and decreases in Bcl-2(+) cells in the mammary gland, compared with the tumor control group. The present study allows a better understanding of the mechanisms (immune and nonimmune) involved in the antitumor effect observed in mice administered kefir or KF. The importance of nonmicrobial components released during milk fermentation to obtain the beneficial antitumor effects is also reported. Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Université de Moncton; Canadá. Universidad Nacional de Tucumán; Argentina Fil: Matar, Chantal. Université de Moncton; Canadá Fil: Farnworth, Edward. Food Research and Development Centre. Agriculture and Agri-Food; Canadá Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Published: 2007

47. Current Review of Genetically Modified Lactic Acid Bacteria for the Prevention and Treatment of Colitis Using Murine Models

Author: Silvina del Carmen, Anderson Miyoshi, Vasco Azevedo, Jean-Marc Chatel, Alejandra de Moreno de LeBlanc, Jean Guy LeBlanc, Philippe Langella, Luis G. Bermúdez-Humarán, Consejo Nacional de Investigaciones Científicas y Técnicas, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Universidade Federal de Minas Gerais, Consejlho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Centro Brasileiro-Argentino de Biotecnologia (CBAB), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT), and ECOS-SUD-MinCyT
Subjects: CIENCIAS MÉDICAS Y DE LA SALUD, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Inflammation, Review Article, Bioinformatics, Biotecnología de la Salud, law.invention, Probiotic, chemistry.chemical_compound, INFLAMMATION, LACTIC ACID BACTERIA, law, medicine, purl.org/becyt/ford/3.4 [https], lcsh:RC799-869, Colitis, Gastrointestinal tract, Hepatology, biology, business.industry, Gastroenterology, biology.organism_classification, medicine.disease, 3. Good health, Genetically modified organism, Lactic acid, GENETICALLY MODIFIED, Cytokine, chemistry, Immunology, purl.org/becyt/ford/3 [https], lcsh:Diseases of the digestive system. Gastroenterology, medicine.symptom, business, Bacteria, Otras Biotecnologías de la Salud
Abstract: Inflammatory Bowel Diseases (IBD) are disorders of the gastrointestinal tract characterized by recurrent inflammation that requires lifelong treatments. Probiotic microorganisms appear as an alternative for these patients; however, probiotic characteristics are strain dependent and each probiotic needs to be tested to understand the underlining mechanisms involved in their beneficial properties. Genetic modification of lactic acid bacteria (LAB) was also described as a tool for new IBD treatments.The first part of this review shows different genetically modified LAB (GM-LAB) described for IBD treatment since 2000.Then, the two principally studied strategies are discussed (i) GM-LAB producing antioxidant enzymes and (ii) GM-LAB producing the anti-inflammatory cytokine IL-10. Different delivery systems, including protein delivery and DNA delivery, will also be discussed. Studies show the efficacy of GM-LAB (using different expression systems) for the prevention and treatment of IBD, highlighting the importance of the bacterial strain selection (with anti-inflammatory innate properties) as a promising alternative. These microorganisms could be used in the near future for the development of therapeutic products with anti-inflammatory properties that can improve the quality of life of IBD patients. Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Chatel, Jean Marc. Institut National de la Recherche Agronomique; Francia Fil: Miyoshi, Anderson. Universidade Federal do Minas Gerais; Brasil Fil: Azevedo, Vasco. Universidade Federal do Minas Gerais; Brasil Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia Fil: Bermudez Humaran, Luis G.. Institut National de la Recherche Agronomique; Francia Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Published: 2015

48. Lactobacillus rhamnosus RC007 with immune-modulatory properties in an experimental model of TNBS-induced colitis in mice. Potential use as a feed additive for pig production

Author: Gisela, García, Cecilia, Dogi, Alejandra, De Moreno De LeBlanc, Lilia, Cavaglieri, and Cecilia, Greco
Subjects: Immunology, Immunology and Allergy
Published: 2015

49. Current Review of Genetically Modified Lactic Acid Bacteria for the Prevention and Treatment of Colitis Using Murine Models

Author: de Moreno de LeBlanc, Alejandra, del Carmen, Silvina, Chatel, Jean-Marc, Miyoshi, Anderson, Azevedo, Vasco, Langella, Philippe, Bermúdez-Humarán, Luis G., and LeBlanc, Jean Guy
Subjects: Article Subject
Abstract: Inflammatory Bowel Diseases (IBD) are disorders of the gastrointestinal tract characterized by recurrent inflammation that requires lifelong treatments. Probiotic microorganisms appear as an alternative for these patients; however, probiotic characteristics are strain dependent and each probiotic needs to be tested to understand the underlining mechanisms involved in their beneficial properties. Genetic modification of lactic acid bacteria (LAB) was also described as a tool for new IBD treatments. The first part of this review shows different genetically modified LAB (GM-LAB) described for IBD treatment since 2000. Then, the two principally studied strategies are discussed (i) GM-LAB producing antioxidant enzymes and (ii) GM-LAB producing the anti-inflammatory cytokine IL-10. Different delivery systems, including protein delivery and DNA delivery, will also be discussed. Studies show the efficacy of GM-LAB (using different expression systems) for the prevention and treatment of IBD, highlighting the importance of the bacterial strain selection (with anti-inflammatory innate properties) as a promising alternative. These microorganisms could be used in the near future for the development of therapeutic products with anti-inflammatory properties that can improve the quality of life of IBD patients.
Published: 2015
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50. Effect of probiotic administration on the intestinal microbiota, current knowledge and potential applications

Author: Alejandra de Moreno de LeBlanc and Jean Guy LeBlanc
Subjects: CIENCIAS MÉDICAS Y DE LA SALUD, Colorectal cancer, Ciencias de la Salud, Disease, Gut flora, Inflammatory bowel disease, law.invention, Feces, Probiotic, purl.org/becyt/ford/3.3 [https], DYSBIOSIS, law, medicine, Animals, Humans, Topic Highlight, Irritable bowel syndrome, biology, Microbiota, Probiotics, GUT MICROBIOTA, SYMBIOSIS, Gastroenterology, Ética Médica, General Medicine, TREATMENT, biology.organism_classification, medicine.disease, Obesity, PROBIOTICS, Biological Therapy, Intestines, Intestinal Diseases, Treatment Outcome, Immunology, purl.org/becyt/ford/3 [https], Dysbiosis
Abstract: Although it is now known that the human body is colonized by a wide variety of microbial populations in different parts (such as the mouth, pharynx and respiratory system, the skin, the gastro- and urogenital tracts), many effects of the complex interactions between the human host and microbial symbionts are still not completely understood. The dysbiosis of the gastrointestinal tract microbiota is considered to be one of the most important contributing factors in the development of many gastrointestinal diseases such as inflammatory bowel disease, irritable bowel syndrome and colorectal cancer, as well as systemic diseases like obesity, diabetes, atherosclerosis and non-alcoholic fatty liver disease. Fecal microbial transplantations appear to be promising therapies for dysbiosis-associated diseases; however, probiotic microorganisms have been growing in popularity due to increasing numbers of studies proving that certain strains present health promoting properties, among them the beneficial balance of the intestinal microbiota. Inflammatory bowel diseases and obesity are the pathologies in which there are more studies showing this beneficial association using animal models and even in human clinical trials. In this review, the association of the human gut microbiota and human health will be discussed along with the benefits that probiotics can confer on this symbiotic activity and on the prevention or treatment of associated diseases. Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Published: 2014

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