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Zebra finch song behavior is sexually dimorphic: males sing and females do not. The neuralsystem underlying this behavior is sexually dimorphic, and this sex difference is easy to quantify.During development, the zebra finch song system can be altered by steroid hormones,specifically estradiol, which actually masculinizes it. Because of the ease of quantification andexperimental manipulation, the zebra finch song system has great potential for use inundergraduate labs.Unfortunately, the underlying costs prohibit use of this system in undergraduate labs. Further, thetime required to perform a developmental study renders such undertakings unrealistic within asingle academic term.We have overcome these barriers by creating digital tools, including an image library of songnuclei from zebra finch brains. Students using this library replicate and extend a publishedexperiment examining the dose of estradiol required to masculinize the female zebra finch brain.We have used this library for several terms, and students not only obtain significant experimentalresults but also make gains in understanding content, experimental controls, and inferentialstatistics (ANOVA and post-hoc tests). We have provided free access to these digital tools athttp://mdcune.psych.ucla.edu/modules/birdsong.

Zebra finch song behavior is sexually dimorphic: males sing and females do not. The neural system underlying this behavior is sexually dimorphic, and this sex difference is easy to quantify. During development, the zebra finch song system can be altered by steroid hormones, specifically estradiol, which actually masculinizes it. Because of the ease of quantification and experimental manipulation, the zebra finch song system has great potential for use in undergraduate labs. Unfortunately, the underlying costs prohibit use of this system in undergraduate labs. Further, the time required to perform a developmental study renders such undertakings unrealistic within a single academic term. We have overcome these barriers by creating digital tools, including an image library of song nuclei from zebra finch brains. Students using this library replicate and extend a published experiment examining the dose of estradiol required to masculinize the female zebra finch brain. We have used this library for several terms, and students not only obtain significant experimental results but also make gains in understanding content, experimental controls, and inferential statistics (analysis of variance and post hoc tests). We have provided free access to these digital tools at the following website: http://mdcune.psych.ucla.edu/modules/birdsong. (Contains 6 figures and 1 table.)