467 results on '"Corcelli A"'
Search Results
2. Correction to Vibrational Spectroscopic Map, Vibrational Spectroscopy, and Intermolecular Interaction
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Baiz, Carlos R, Błasiak, Bartosz, Bredenbeck, Jens, Cho, Minhaeng, Choi, Jun-Ho, Corcelli, Steven A, Dijkstra, Arend G, Feng, Chi-Jui, Garrett-Roe, Sean, Ge, Nien-Hui, Hanson-Heine, Magnus WD, Hirst, Jonathan D, Jansen, Thomas LC, Kwac, Kijeong, Kubarych, Kevin J, Londergan, Casey H, Maekawa, Hiroaki, Reppert, Mike, Saito, Shinji, Roy, Santanu, Skinner, James L, Stock, Gerhard, Straub, John E, Thielges, Megan C, Tominaga, Keisuke, Tokmakoff, Andrei, Torii, Hajime, Wang, Lu, Webb, Lauren J, and Zanni, Martin T
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Chemical Sciences ,General Chemistry - Abstract
The authors make the following additions and corrections to the paper, C. Baiz et al., Vibrational Spectroscopic Map, Vibrational Spectroscopy, and Intermolecular Interaction. Chem. Rev. 2020, 120, 7152?7218. 1. The below funding sources should be added to the Acknowledgments section on pages 7204 and 7205: NHG acknowledges support from the National Science Foundation (CHE-1905395). AT thanks the Advanced Materials for Energy-Water Systems (AMEWS) Center (DE-AC02-06CH11357) for support. Thus, the Acknowledgments should be as follows: This work was supported by IBS-R023-D1 (MC). BB wishes to thank the European Union’s Horizon 2020 under the Marie Sk?odowska-Curie Grant Agreement No. 665778, as well as the National Science Centre, Poland (grant no. 2016/23/P/ST4/01720). CRB acknowledges generous support from the National Science Foundation (CHE-847199, BIO-1815354 ) , the National Ins t i t u tes o f Hea l t h (R35GM133359), and the Welch Foundation (F-1891). NHG acknowledges support from the National Science Foundation (CHE-1905395). MWDH-H and JDH thank the University of Nottingham, Green Chemicals Beacon for funding toward this research. AT thanks the Advanced Materials for Energy-Water Systems (AMEWS) Center (DEAC02-06CH11357) for support. MCT acknowledges support from Department of Energy (DE-SC0018983), National Science Foundation (1552996), and National Institutes of Health (GM114500). AGD would like to thank Prof. Andrei Tokmakoff for discussing amide modes when he was a Ph.D. student. LW acknowledges the support from the National Institutes of Health through Award R01GM130697. SAC acknowledges support from National Science Foundation (CHE-1565471). 2. The affiliations of Minhaeng Cho and Kijeong Kwak on page 7201 should be corrected as follows: Minhaeng Cho ? Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science (IBS), Seoul 02841, Republic of Korea; Department of Chemistry, Korea University, Seoul 02841, Republic of Korea Kijeong Kwac ? Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science (IBS), Seoul 02841, Republic of Korea 3. Reference 329 on page 7214 should be replaced with the following paper: (329) Sul, S; Karaiskaj, D.; Jiang Y.; Ge, N.-H. Conformations of N-Acetyl-L-Prolinamide by Two-Dimensional Infrared Spectroscopy. J. Phys. Chem. B 2006, 110, 19891?19905. 4. A correction in the biography of Magnus Hanson-Heine on page 7203 should be made. MH-H received his Ph.D. in 2014, not 2010.
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- 2021
3. A CRISPR/Cas9-generated mutation in the zebrafish orthologue of PPP2R3B causes idiopathic scoliosis
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Marian Seda, Berta Crespo, Michelangelo Corcelli, Daniel P. Osborn, and Dagan Jenkins
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Medicine ,Science - Abstract
Abstract Idiopathic scoliosis (IS) is the deformation and/or abnormal curvature of the spine that develops progressively after birth. It is a very common condition, affecting approximately 4% of the general population, yet the genetic and mechanistic causes of IS are poorly understood. Here, we focus on PPP2R3B, which encodes a protein phosphatase 2A regulatory subunit. We found that PPP2R3B is expressed at sites of chondrogenesis within human foetuses, including the vertebrae. We also demonstrated prominent expression in myotome and muscle fibres in human foetuses, and zebrafish embryos and adolescents. As there is no rodent orthologue of PPP2R3B, we used CRIPSR/Cas9-mediated gene-editing to generate a series of frameshift mutations in zebrafish ppp2r3b. Adolescent zebrafish that were homozygous for this mutation exhibited a fully penetrant kyphoscoliosis phenotype which became progressively worse over time, mirroring IS in humans. These defects were associated with reduced mineralisation of vertebrae, resembling osteoporosis. Electron microscopy demonstrated abnormal mitochondria adjacent to muscle fibres. In summary, we report a novel zebrafish model of IS and reduced bone mineral density. In future, it will be necessary to delineate the aetiology of these defects in relation to bone, muscle, neuronal and ependymal cilia function.
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- 2023
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4. Provimento da direção escolar nas Etecs do Centro Paula Souza
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Edmeire Ferreira Corcelli and Paulo Roberto Prado Constantino
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direção escolar ,gestão e administração escolar ,educação professional ,políticas públicas ,Industrial engineering. Management engineering ,T55.4-60.8 ,Management. Industrial management ,HD28-70 - Abstract
Neste artigo introduzimos a temática do provimento da função de direção nas escolas técnicas estaduais [Etecs] do Centro Estadual de Educação Tecnológica Paula Souza (CEETEPS ou Centro Paula Souza), refletindo sobre os princípios democráticos da gestão escolar aplicados ao contexto da educação profissional técnica de nível médio. Como parte de uma pesquisa em desenvolvimento no Programa de Mestrado Profissional em Gestão e Desenvolvimento da Educação Profissional do CEETEPS, o objetivo fundamental é investigar o processo de qualificação e eleição dos dirigentes das Etecs, discutindo-o à luz dos preceitos da democratização das instituições públicas de ensino.
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- 2023
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5. Case report: Variability in clinical features as a potential pitfall for the diagnosis of Barth syndrome
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Nicola Tovaglieri, Silvia Russo, Emanuele Micaglio, Angela Corcelli, and Simona Lobasso
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rare X-linked disease ,TAFAZZIN ,cardiolipin remodeling ,cardiomyopathy ,neutropenia ,Pediatrics ,RJ1-570 - Abstract
BackgroundBarth syndrome is a rare genetic disease characterized by cardiomyopathy, skeletal muscle weakness, neutropenia, growth retardation and organic aciduria. This variable phenotype is caused by pathogenic hemizygous variants of the TAFAZZIN gene on the X chromosome, which impair metabolism of the mitochondrial phospholipid cardiolipin. Although most patients are usually diagnosed in the first years of life, the extremely variable clinical picture and the wide range of clinical presentations may both delay diagnosis. This is the case reported here of a man affected with severe neutropenia, who was not diagnosed with Barth syndrome until adulthood.Case presentationWe describe herein a family case, specifically two Caucasian male cousins sharing the same mutation in the TAFAZZIN gene with a wide phenotypic variability: an infant who was early diagnosed with Barth syndrome due to heart failure, and his maternal cousin with milder and extremely different clinical features who has received the same diagnosis only at 33 years of age.ConclusionsOur report supports the underestimation of the prevalence of Barth syndrome, which should be always considered in the differential diagnosis of male patients with recurrent neutropenia with or without signs and symptoms of cardiomyopathy.
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- 2023
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6. Vibrational Spectroscopic Map, Vibrational Spectroscopy, and Intermolecular Interaction
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Baiz, Carlos R, Błasiak, Bartosz, Bredenbeck, Jens, Cho, Minhaeng, Choi, Jun-Ho, Corcelli, Steven A, Dijkstra, Arend G, Feng, Chi-Jui, Garrett-Roe, Sean, Ge, Nien-Hui, Hanson-Heine, Magnus WD, Hirst, Jonathan D, Jansen, Thomas LC, Kwac, Kijeong, Kubarych, Kevin J, Londergan, Casey H, Maekawa, Hiroaki, Reppert, Mike, Saito, Shinji, Roy, Santanu, Skinner, James L, Stock, Gerhard, Straub, John E, Thielges, Megan C, Tominaga, Keisuke, Tokmakoff, Andrei, Torii, Hajime, Wang, Lu, Webb, Lauren J, and Zanni, Martin T
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Bioengineering ,Humans ,Models ,Chemical ,Proteins ,Spectrum Analysis ,Spectrum Analysis ,Raman ,Static Electricity ,Vibration ,Chemical Sciences ,General Chemistry - Abstract
Vibrational spectroscopy is an essential tool in chemical analyses, biological assays, and studies of functional materials. Over the past decade, various coherent nonlinear vibrational spectroscopic techniques have been developed and enabled researchers to study time-correlations of the fluctuating frequencies that are directly related to solute-solvent dynamics, dynamical changes in molecular conformations and local electrostatic environments, chemical and biochemical reactions, protein structural dynamics and functions, characteristic processes of functional materials, and so on. In order to gain incisive and quantitative information on the local electrostatic environment, molecular conformation, protein structure and interprotein contacts, ligand binding kinetics, and electric and optical properties of functional materials, a variety of vibrational probes have been developed and site-specifically incorporated into molecular, biological, and material systems for time-resolved vibrational spectroscopic investigation. However, still, an all-encompassing theory that describes the vibrational solvatochromism, electrochromism, and dynamic fluctuation of vibrational frequencies has not been completely established mainly due to the intrinsic complexity of intermolecular interactions in condensed phases. In particular, the amount of data obtained from the linear and nonlinear vibrational spectroscopic experiments has been rapidly increasing, but the lack of a quantitative method to interpret these measurements has been one major obstacle in broadening the applications of these methods. Among various theoretical models, one of the most successful approaches is a semiempirical model generally referred to as the vibrational spectroscopic map that is based on a rigorous theory of intermolecular interactions. Recently, genetic algorithm, neural network, and machine learning approaches have been applied to the development of vibrational solvatochromism theory. In this review, we provide comprehensive descriptions of the theoretical foundation and various examples showing its extraordinary successes in the interpretations of experimental observations. In addition, a brief introduction to a newly created repository Web site (http://frequencymap.org) for vibrational spectroscopic maps is presented. We anticipate that a combination of the vibrational frequency map approach and state-of-the-art multidimensional vibrational spectroscopy will be one of the most fruitful ways to study the structure and dynamics of chemical, biological, and functional molecular systems in the future.
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- 2020
7. Beneficial effects of SS-31 peptide on cardiac mitochondrial dysfunction in tafazzin knockdown mice
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Silvia Russo, Domenico De Rasmo, Anna Signorile, Angela Corcelli, and Simona Lobasso
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Medicine ,Science - Abstract
Abstract Barth Syndrome (BTHS), a genetic disease associated with early-onset cardioskeletal myopathy, is caused by loss-of-function mutations of the TAFAZZIN gene, which is responsible for remodeling the mitochondrial phospholipid cardiolipin (CL). Deregulation of CL biosynthesis and maturation in BTHS mitochondria result in a dramatically increased monolysocardiolipin (MLCL)/CL ratio associated with bioenergetic dysfunction. One of the most promising therapeutic approaches for BTHS includes the mitochondria-targeted tetrapeptide SS-31, which interacts with CL. Here, we used TAFAZZIN knockdown (TazKD) mice to investigate for the first time whether in vivo administration of SS-31 could affect phospholipid profiles and mitochondrial dysfunction. The CL fingerprinting of TazKD cardiac mitochondria obtained by MALDI-TOF/MS revealed the typical lipid changes associated with BTHS. TazKD mitochondria showed lower respiratory rates in state 3 and 4 together with a decreased in maximal respiratory rates. Treatment of TazKD mice with SS-31 improved mitochondrial respiratory capacity and promoted supercomplex organization, without affecting the MLCL/CL ratio. We hypothesize that SS-31 exerts its effect by influencing the function of the respiratory chain rather than affecting CL directly. In conclusion, our results indicate that SS-31 have beneficial effects on improving cardiac mitochondrial dysfunction in a BTHS animal model, suggesting the peptide as future pharmacologic agent for therapy.
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- 2022
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8. BONE ANABOLIC POTENTIAL OF EXTRACELLULAR VESICLES SECRETED BY HUMAN INDUCED PLURIPOTENT STEM CELL-DERIVED MESENCHYMAL STEM CELLS.
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Guillot, P.V., primary, Petzendorfer, E., additional, HASAN, M.M., additional, and CORCELLI, M., additional
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- 2024
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9. Beneficial effects of SS-31 peptide on cardiac mitochondrial dysfunction in tafazzin knockdown mice
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Russo, Silvia, De Rasmo, Domenico, Signorile, Anna, Corcelli, Angela, and Lobasso, Simona
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- 2022
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10. A Lipidomic Approach to Identify Potential Biomarkers in Exosomes From Melanoma Cells With Different Metastatic Potential
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Simona Lobasso, Paola Tanzarella, Francesco Mannavola, Marco Tucci, Francesco Silvestris, Claudia Felici, Chiara Ingrosso, Angela Corcelli, and Patrizia Lopalco
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melanoma ,membrane vesicles ,osteotropism ,lipids ,MALDI-TOF/MS ,Physiology ,QP1-981 - Abstract
Melanoma, one of the most lethal cutaneous cancers, is characterized by its ability to metastasize to other distant sites, such as the bone. Melanoma cells revealed a variable in vitro propensity to be attracted toward bone fragments, and melanoma-derived exosomes play a role in regulating the osteotropism of these cells. We have here investigated the lipid profiles of melanoma cell lines (LCP and SK-Mel28) characterized by different metastatic propensities to colonize the bone. We have purified exosomes from cell supernatants by ultracentrifugation, and their lipid composition has been compared to identify potential lipid biomarkers for different migration and invasiveness of melanoma cells. Matrix-assisted laser desorption ionization-time-of-flight/mass spectrometry (MALDI-TOF/MS) lipid analysis has been performed on very small amounts of intact parental cells and exosomes by skipping lipid extraction and separation steps. Statistical analysis has been applied to MALDI mass spectra in order to discover significant differences in lipid profiles. Our results clearly show more saturated and shorter fatty acid tails in poorly metastatic (LCP) cells compared with highly metastatic (SK-Mel28) cells, particularly for some species of phosphatidylinositol. Sphingomyelin, lysophosphatidylcholine, and phosphatidic acid were enriched in exosome membranes compared to parental cells. In addition, we have clearly detected a peculiar phospholipid bis(monoacylglycero)phosphate as a specific lipid marker of exosomes. MALDI-TOF/MS lipid profiles of exosomes derived from the poorly and highly metastatic cells were not significantly different.
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- 2021
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11. Terrestrial transport modalities in China concerning monetary, energy and environmental costs
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Huang, Shupei, An, Haizhong, Viglia, Silvio, Fiorentino, Gabriella, Corcelli, Fabiana, Fang, Wei, and Ulgiati, Sergio
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- 2018
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12. Cross-species complementation of bacterial- and eukaryotic-type cardiolipin synthases
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Petra Gottier, Mauro Serricchio, Rita Vitale, Angela Corcelli, and Peter Bütikofer
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cardiolipin synthase ,cardiolipin ,Trypanosoma brucei ,Saccharomyces cerevisiae ,phospholipids ,mitochondria ,Biology (General) ,QH301-705.5 - Abstract
The glycerophospholipid cardiolipin is a unique constituent of bacterial and mitochondrial membranes. It is involved in forming and stabilizing high molecular mass membrane protein complexes and in maintaining membrane architecture. Absence of cardiolipin leads to reduced efficiency of the electron transport chain, decreased membrane potential, and, ultimately, impaired respiratory metabolism. For the protozoan parasite Trypanosoma brucei cardiolipin synthesis is essential for survival, indicating that the enzymes involved in cardiolipin production represent potential drug targets. T. brucei cardiolipin synthase (TbCLS) is unique as it belongs to the family of phospholipases D (PLD), harboring a prokaryotic-type cardiolipin synthase (CLS) active site domain. In contrast, most other eukaryotic CLS, including the yeast ortholog ScCrd1, are members of the CDP-alcohol phosphatidyl transferase family. To study if these mechanistically distinct CLS enzymes are able to catalyze cardiolipin production in a cell that normally expresses a different type of CLS, we expressed TbCLS and ScCrd1 in CLS-deficient yeast and trypanosome strains, respectively. Our results show that TbCLS complemented cardiolipin production in CRD1 knockout yeast and partly restored wild-type colony forming capability under stress conditions. Remarkably, CL remodeling appeared to be impaired in the transgenic construct, suggesting that CL production and remodeling are tightly coupled processes that may require a clustering of the involved proteins into specific CL-synthesizing domains. In contrast, no complementation was observed by heterologous expression of ScCrd1 in conditional TbCLS knockout trypanosomes, despite proper mitochondrial targeting of the protein.
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- 2017
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13. End-of-life treatment of crystalline silicon photovoltaic panels. An emergy-based case study
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Corcelli, F., Ripa, M., and Ulgiati, S.
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- 2017
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14. DNA minor-groove binder Hoechst 33258 destabilizes base-pairing adjacent to its binding site
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Zhang, Xin-Xing, Brantley, Shelby L., Corcelli, Steven A., and Tokmakoff, Andrei
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- 2020
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15. Boundary Integral Methods for the Poisson Equation of Continuum Dielectric Solvation Models
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Pratt, L. R., Tawa, G. J., Hummer, G., Garcia, A. E., and Corcelli, S. A.
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Physics - Chemical Physics - Abstract
This paper tests a dielectric model for variation of hydration free energy with geometry of complex solutes in water. It works out some basic aspects of the theory of boundary integral methods for these problems. One aspect of the algorithmic discussion lays the basis for multigrid methods of solution, methods that are likely to be necessary for similarly accurate numerical solution of these models for much larger solutes. Other aspects of the algorithmic work show how macroscopic surfaces such as solution interfaces and membranes may be incorporated and also show how these methods can be transferred directly to periodic boundary conditions. This dielectric model is found to give interesting and helpful results for the variation in solvation free energy with solute geometry. However, it typically significantly over-stabilizes classic attractive ion-pairing configurations. On the basis of the examples and algorithmic considerations, we make some observations about extension of this continuum model incrementally to reintroduce molecular detail of the solvation structure., Comment: presented at the Martin Karplus birthday symposium, 3/95; revtex with jpc.sty, 46 pages, 9 figures
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- 1995
16. Mixed Direct-Iterative Methods for Boundary Integral Formulations of Dielectric Solvation Models
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Corcelli, S. A., Kress, J. D., Pratt, L. R., and Tawa, G. J.
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Physics - Chemical Physics - Abstract
This paper describes a mixed direct-iterative method for boundary integral formulations of dielectric solvation models. We give an example for which a direct solution at thermal accuracy is nontrivial and for which Gauss-Seidel iteration diverges in rare but reproducible cases. This difficulty is analyzed by obtaining the eigenvalues and the spectral radius of the iteration matrix. This establishes that the nonconvergence is due to inaccuracies of the asymptotic approximations for the matrix elements for accidentally close boundary element pairs on different spheres. This difficulty is cured by checking for boundary element pairs closer than the typical spatial extent of the boundary elements and for those pairs performing an `in-line' Monte Carlo integration to evaluate the required matrix elements. This difficulty are not expected and have not been observed when only a direct solution is sought. Finally, we give an example application of these methods to deprotonation of monosilicic acid in water., Comment: to be published in proceeding of the Pacific Symposium on Biomcomputing; latex with sprocl.sty, 17 pages with 3 figures included with psfig
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- 1995
17. Non-intuitive clustering of 9,10-phenanthrenequinone on Au(111)
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Ryan D. Brown, Rebecca C. Quardokus, Natalie A. Wasio, Jacob P. Petersen, Angela M. Silski, Steven A. Corcelli, and S. Alex Kandel
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metastable clusters ,9,10-phenanthrenequinone ,scanning tunneling microscopy ,self-assembly ,Technology ,Chemical technology ,TP1-1185 ,Science ,Physics ,QC1-999 - Abstract
The direct injection of a 9,10-phenanthrenequinone in tetrahydrofuran solution on a Au(111) substrate in high vacuum results in the formation of metastable clusters with a non-intuitive structure. Metastable, rectangular tetramers of this molecule form in which the net molecular dipoles all orient toward the center of the cluster. This structure does not allow for additional hydrogen bonding and thus the origin of its metastability is not clear. We compare this feature to other structures observed on this surface, as well as those formed during the deposition of 9-fluorenone, which does not exhibit this anomalous clustering behavior.
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- 2017
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18. Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii
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Patrizia Lopalco, Julia Stahl, Cosimo Annese, Beate Averhoff, and Angela Corcelli
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Medicine ,Science - Abstract
Abstract Acidic glycerophospholipids play an important role in determining the resistance of Gram-negative bacteria to stress conditions and antibiotics. Acinetobacter baumannii, an opportunistic human pathogen which is responsible for an increasing number of nosocomial infections, exhibits broad antibiotic resistances. Here lipids of A. baumannii have been analyzed by combined MALDI-TOF/MS and TLC analyses; in addition GC-MS analyses of fatty acid methyl esters released by methanolysis of membrane phospholipids have been performed. The main glycerophospholipids are phosphatidylethanolamine, phosphatidylglycerol, acyl-phosphatidylglycerol and cardiolipin together with monolysocardiolipin, a lysophospholipid only rarely detected in bacterial membranes. The major acyl chains in the phospholipids are C16:0 and C18:1, plus minor amounts of short chain fatty acids. The structures of the cardiolipin and monolysocardiolipin have been elucidated by post source decay mass spectrometry analysis. A large variety of cardiolipin and monolysocardiolipin species were found in A. baumannii. Similar lysocardiolipin levels were found in the two clinical strains A. baumannii ATCC19606 T and AYE whereas in the nonpathogenic strain Acinetobacter baylyi ADP1 lysocardiolipin levels were highly reduced.
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- 2017
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19. DNA’s Chiral Spine of Hydration
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M. Luke McDermott, Heather Vanselous, Steven A. Corcelli, and Poul B. Petersen
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Chemistry ,QD1-999 - Published
- 2017
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20. Provimento da direção escolar nas Etecs do Centro Paula Souza: aproximações sobre a gestão democrática na educação profissional
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Corcelli, Edmeire Ferreira, Constantino, Paulo Roberto Prado, Corcelli, Edmeire Ferreira, and Constantino, Paulo Roberto Prado
- Abstract
Neste artigo introduzimos a temática do provimento da função de direção nas escolas técnicas estaduais [Etecs] do Centro Estadual de Educação Tecnológica Paula Souza (CEETEPS ou Centro Paula Souza), refletindo sobre os princípios democráticos da gestão escolar aplicados ao contexto da educação profissional técnica de nível médio. Como parte de uma pesquisa em desenvolvimento no Programa de Mestrado Profissional em Gestão e Desenvolvimento da Educação Profissional do CEETEPS, o objetivo fundamental é investigar o processo de qualificação e eleição dos dirigentes das Etecs, discutindo-o à luz dos preceitos da democratização das instituições públicas de ensino.
- Published
- 2023
21. Alteration of Cholesterol Sulfate/Seminolipid Ratio in Semen Lipid Profile of Men With Oligoasthenozoospermia
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Patrizia Lopalco, Rita Vitale, Yoon Sung Cho, Pasquale Totaro, Angela Corcelli, and Simona Lobasso
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oligoasthenozoospermia ,cholesterol sulfate ,lipids ,MALDI-TOF/MS ,sperm ,Physiology ,QP1-981 - Abstract
The reduction of sperm motility and count, or oligoasthenozoospermia, is one of the major causes of reduced fertility or infertility in men. Lipid composition of spermatozoa is important in determining their functional characteristics, in particular on motility, acrosomal exocytosis or fusogenic properties of the sperm. Here we investigated the levels of semen lipids in 11 infertile patients with severe oligoasthenozoospermia and 9 normozoospermic subjects with normal motility values. Sperm polar and neutral lipids were analyzed by thin-layer chromatography (TLC) and matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Semen of patients with oligoasthenozoospermia showed a reduction of the degree of fatty acid unsaturation in the phospholipids chains that might affect the membrane fluidity. Furthermore, a significant higher cholesterol sulfate/seminolipid ratio was found in semen of oligoasthenozoospermic patients than in subjects with normal motility values, suggesting a critical role of sulfolipids in semen quality. The results may facilitate the understanding of the role of lipids on male fertility and offer interesting perspectives to find innovative treatments for oligoasthenozoospermia.
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- 2019
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22. Dynamically Driven Allostery in MHC Proteins: Peptide-Dependent Tuning of Class I MHC Global Flexibility
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Cory M. Ayres, Esam T. Abualrous, Alistair Bailey, Christian Abraham, Lance M. Hellman, Steven A. Corcelli, Frank Noé, Tim Elliott, and Brian M. Baker
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class I MHC molecules ,peptides ,dynamics ,motion ,allostery ,structure ,Immunologic diseases. Allergy ,RC581-607 - Abstract
T cell receptor (TCR) recognition of antigenic peptides bound and presented by class I major histocompatibility complex (MHC) proteins underlies the cytotoxic immune response to diseased cells. Crystallographic structures of TCR-peptide/MHC complexes have demonstrated how TCRs simultaneously interact with both the peptide and the MHC protein. However, it is increasingly recognized that, beyond serving as a static platform for peptide presentation, the physical properties of class I MHC proteins are tuned by different peptides in ways that are not always structurally visible. These include MHC protein motions, or dynamics, which are believed to influence interactions with a variety of MHC-binding proteins, including not only TCRs, but other activating and inhibitory receptors as well as components of the peptide loading machinery. Here, we investigated the mechanisms by which peptides tune the dynamics of the common class I MHC protein HLA-A2. By examining more than 50 lengthy molecular dynamics simulations of HLA-A2 presenting different peptides, we identified regions susceptible to dynamic tuning, including regions in the peptide binding domain as well as the distal α3 domain. Further analyses of the simulations illuminated mechanisms by which the influences of different peptides are communicated throughout the protein, and involve regions of the peptide binding groove, the β2-microglobulin subunit, and the α3 domain. Overall, our results demonstrate that the class I MHC protein is a highly tunable peptide sensor whose physical properties vary considerably with bound peptide. Our data provides insight into the underlying principles and suggest a role for dynamically driven allostery in the immunological function of MHC proteins.
- Published
- 2019
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23. Lipid Profile Changes During the Development of Artemia franciscana, From Cysts to the First Two Naupliar Stages
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Patrizia Lopalco, Simona Lobasso, Ruy Miguel Alfama Lopes-dos-Santos, Gilbert Van Stappen, and Angela Corcelli
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Artemia franciscana ,phospholipids ,lysophospholipids ,neutral lipids ,MALDI-TOF/MS ,TLC ,Physiology ,QP1-981 - Abstract
The brine shrimp Artemia is an interesting experimental system for studies of developmental processes. Hatching of dormant cysts gives rise to shrimp larvae called nauplii, characterized by numerous naupliar stages representing the first forms of brine shrimp life cycle. Here combined Thin Layer Chromatography (TLC) and Matrix-Assisted Laser Desorption Ionization-Time-of-Flight/Mass Spectrometry (MALDI-TOF/MS) analyses have been performed to gain information on the lipid profiles of cysts and two naupliar stages. Lipid bands isolated after preparative TLC of the lipid extracts have been analyzed to detect various species of each lipid class; in addition Post-Source Decay (PSD) analyses allowed the identification of phospholipid chains. We compared the relative abundance of various polar and neutral lipid species in the lipid extracts, proving for the first time that during the development of nauplii there is an increase of cardiolipin (CL) and lysophospholipid levels; in parallel, the amount of phosphatidylcholine (PC) decreases. In addition, as regards neutral lipids, we found an increase of diacylglycerols (DAGs) in correspondence of the decrease of triacylglycerols (TAGs). Data reflect the fact that naupliar stages, being an active form of life, are more metabolically active and offer a platform to develop further studies on the importance of lipid metabolic pathways and bioactive lipids during the development.
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- 2019
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24. Serotonin Is Involved in Autoimmune Arthritis through Th17 Immunity and Bone Resorption
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Chabbi-Achengli, Yasmine, Coman, Tereza, Collet, Corinne, Callebert, Jacques, Corcelli, Michelangelo, Lin, Hilène, Rignault, Rachel, Dy, Michel, de Vernejoul, Marie-Christine, and Côté, Francine
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- 2016
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25. A CRISPR/Cas9-generated mutation in the zebrafish orthologue of PPP2R3B causes idiopathic scoliosis
- Author
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Marian Seda, Berta Crespo, Michelangelo Corcelli, Daniel P. Osborn, and Dagan Jenkins
- Subjects
Multidisciplinary - Abstract
Idiopathic scoliosis (IS) is the deformation and/or abnormal curvature of the spine that develops progressively after birth. It is a very common condition, affecting approximately 4% of the general population, yet the genetic and mechanistic causes of IS are poorly understood. Here, we focus on PPP2R3B, which encodes a protein phosphatase 2A regulatory subunit. We found that PPP2R3B is expressed at sites of chondrogenesis within human foetuses, including the vertebrae. We also demonstrated prominent expression in myotome and muscle fibres in human foetuses, and zebrafish embryos and adolescents. As there is no rodent orthologue of PPP2R3B, we used CRIPSR/Cas9-mediated gene-editing to generate a series of frameshift mutations in zebrafish ppp2r3b. Adolescent zebrafish that were homozygous for this mutation exhibited a fully penetrant kyphoscoliosis phenotype which became progressively worse over time, mirroring IS in humans. These defects were associated with reduced mineralisation of vertebrae, resembling osteoporosis. Electron microscopy demonstrated abnormal mitochondria adjacent to muscle fibres. In summary, we report a novel zebrafish model of IS and reduced bone mineral density. In future, it will be necessary to delineate the aetiology of these defects in relation to bone, muscle, neuronal and ependymal cilia function.
- Published
- 2022
26. Structural and Functional Maturation of Rat Primary Motor Cortex Layer V Neurons
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Bruno Benedetti, Dominik Dannehl, Jan Maximilian Janssen, Corinna Corcelli, Sébastien Couillard-Després, and Maren Engelhardt
- Subjects
axon initial segment (AIS) ,maturation ,motor cortex ,development ,patch clamp ,motor neurons ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Rodent neocortical neurons undergo prominent postnatal development and maturation. The process is associated with structural and functional maturation of the axon initial segment (AIS), the site of action potential initiation. In this regard, cell size and optimal AIS length are interconnected. In sensory cortices, developmental onset of sensory input and consequent changes in network activity cause phasic AIS plasticity that can also control functional output. In non-sensory cortices, network input driving phasic events should be less prominent. We, therefore, explored the relationship between postnatal functional maturation and AIS maturation in principal neurons of the primary motor cortex layer V (M1LV), a non-sensory area of the rat brain. We hypothesized that a rather continuous process of AIS maturation and elongation would reflect cell growth, accompanied by progressive refinement of functional output properties. We found that, in the first two postnatal weeks, cell growth prompted substantial decline of neuronal input resistance, such that older neurons needed larger input current to reach rheobase and fire action potentials. In the same period, we observed the most prominent AIS elongation and significant maturation of functional output properties. Alternating phases of AIS plasticity did not occur, and changes in functional output properties were largely justified by AIS elongation. From the third postnatal week up to five months of age, cell growth, AIS elongation, and functional output maturation were marginal. Thus, AIS maturation in M1LV is a continuous process that attunes the functional output of pyramidal neurons and associates with early postnatal development to counterbalance increasing electrical leakage due to cell growth.
- Published
- 2020
- Full Text
- View/download PDF
27. Understanding Activation Patterns in Shared Circuits: Toward a Value Driven Model
- Author
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Lisa Aziz-Zadeh, Emily Kilroy, and Giorgio Corcelli
- Subjects
mirror neuron system ,motor learning ,social cognition ,shared neural networks ,value-based decision making ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Over the past decade many studies indicate that we utilize our own motor system to understand the actions of other people. This mirror neuron system (MNS) has been proposed to be involved in social cognition and motor learning. However, conflicting findings regarding the underlying mechanisms that drive these shared circuits make it difficult to decipher a common model of their function. Here we propose adapting a “value-driven” model to explain discrepancies in the human mirror system literature and to incorporate this model with existing models. We will use this model to explain discrepant activation patterns in multiple shared circuits in the human data, such that a unified model may explain reported activation patterns from previous studies as a function of value.
- Published
- 2018
- Full Text
- View/download PDF
28. Heterogeneity of the Axon Initial Segment in Interneurons and Pyramidal Cells of Rodent Visual Cortex
- Author
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Felix Höfflin, Alexander Jack, Christian Riedel, Julia Mack-Bucher, Johannes Roos, Corinna Corcelli, Christian Schultz, Petra Wahle, and Maren Engelhardt
- Subjects
axon initial segment ,interneuron ,pyramidal neuron ,βIV-spectrin ,axon-carrying dendrite cell ,basket cell ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
The microdomain that orchestrates action potential initiation in neurons is the axon initial segment (AIS). It has long been considered to be a rather homogeneous domain at the very proximal axon hillock with relatively stable length, particularly in cortical pyramidal cells. However, studies in other brain regions paint a different picture. In hippocampal CA1, up to 50% of axons emerge from basal dendrites. Further, in about 30% of thick-tufted layer V pyramidal neurons in rat somatosensory cortex, axons have a dendritic origin. Consequently, the AIS is separated from the soma. Recent in vitro and in vivo studies have shown that cellular excitability is a function of AIS length/position and somatodendritic morphology, undermining a potentially significant impact of AIS heterogeneity for neuronal function. We therefore investigated neocortical axon morphology and AIS composition, hypothesizing that the initial observation of seemingly homogeneous AIS is inadequate and needs to take into account neuronal cell types. Here, we biolistically transfected cortical neurons in organotypic cultures to visualize the entire neuron and classify cell types in combination with immunolabeling against AIS markers. Using confocal microscopy and morphometric analysis, we investigated axon origin, AIS position, length, diameter as well as distance to the soma. We find a substantial AIS heterogeneity in visual cortical neurons, classified into three groups: (I) axons with somatic origin with proximal AIS at the axon hillock; (II) axons with somatic origin with distal AIS, with a discernible gap between the AIS and the soma; and (III) axons with dendritic origin (axon-carrying dendrite cell, AcD cell) and an AIS either starting directly at the axon origin or more distal to that point. Pyramidal cells have significantly longer AIS than interneurons. Interneurons with vertical columnar axonal projections have significantly more distal AIS locations than all other cells with their prevailing phenotype as an AcD cell. In contrast, neurons with perisomatic terminations display most often an axon originating from the soma. Our data contribute to the emerging understanding that AIS morphology is highly variable, and potentially a function of the cell type.
- Published
- 2017
- Full Text
- View/download PDF
29. Cardiolipin fingerprinting of leukocytes by MALDI-TOF/MS as a screening tool for Barth syndrome[S]
- Author
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Roberto Angelini, Simona Lobasso, Ruggiero Gorgoglione, Ann Bowron, Colin G. Steward, and Angela Corcelli
- Subjects
cardiomyopathy ,lysophospholipids ,mass spectrometry ,matrix-assisted laser desorption/ionization ,mitochondria ,phospholipids ,Biochemistry ,QD415-436 - Abstract
Barth syndrome (BTHS), an X-linked disease associated with cardioskeletal myopathy, neutropenia, and organic aciduria, is characterized by abnormalities of cardiolipin (CL) species in mitochondria. Diagnosis of the disease is often compromised by lack of rapid and widely available diagnostic laboratory tests. The present study describes a new method for BTHS screening based on MALDI-TOF/MS analysis of leukocyte lipids. This generates a “CL fingerprint” and allows quick and simple assay of the relative levels of CL and monolysocardiolipin species in leukocyte total lipid profiles. To validate the method, we used vector algebra to analyze the difference in lipid composition between controls (24 healthy donors) and patients (8 boys affected by BTHS) in the high-mass phospholipid range. The method of lipid analysis described represents an important additional tool for the diagnosis of BTHS and potentially enables therapeutic monitoring of drug targets, which have been shown to ameliorate abnormal CL profiles in cells.
- Published
- 2015
- Full Text
- View/download PDF
30. Haloferax volcanii, as a Novel Tool for Producing Mammalian Olfactory Receptors Embedded in Archaeal Lipid Bilayer
- Author
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Simona Lobasso, Rita Vitale, Patrizia Lopalco, and Angela Corcelli
- Subjects
extremely halophilic microorganisms ,archaeal lipids ,archaeonanosomes ,olfactory receptors ,biosensors ,Science - Abstract
The aim of this study was to explore the possibility of using an archaeal microorganism as a host system for expressing mammalian olfactory receptors (ORs). We have selected the archaeon Haloferax volcanii as a cell host system and one of the most extensively investigated OR, namely I7-OR, whose preferred ligands are short-chain aldehydes, such as octanal, heptanal, nonanal. A novel plasmid has been constructed to express the rat I7-OR, fused with a hexahistidine-tag for protein immunodetection. The presence of the recombinant receptor at a membrane level was demonstrated by immunoblot of the membranes isolated from the transgenic archaeal strain. In addition, the lipid composition of archaeonanosomes containing ORs has been characterized in detail by High-Performance Thin-Layer Chromatography (HPTLC) in combination with Matrix-Assisted Laser Desorption Ionization—Time-Of-Flight/Mass Spectrometry (MALDI-TOF/MS) analysis.
- Published
- 2015
- Full Text
- View/download PDF
31. Local Electric Fields in Aqueous Electrolytes
- Author
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Arnaldo L. Serrano, Chad I. Drexler, Paul S. Cremer, Olivia M. Cracchiolo, Halil I. Okur, Steven A. Corcelli, Ryan L. Myers, and Okur, Halil İbrahim
- Subjects
Ions ,Anions ,chemistry.chemical_classification ,Aqueous solution ,Hofmeister series ,Water ,Infrared spectroscopy ,Salt (chemistry) ,Ketones ,Molecular Dynamics Simulation ,Chromophore ,Photochemistry ,Surfaces, Coatings and Films ,Ion ,Electrolytes ,symbols.namesake ,Stark effect ,chemistry ,Cations ,Electric field ,Materials Chemistry ,symbols ,Salts ,Probes ,Physical and Theoretical Chemistry - Abstract
Vibrational Stark shifts were explored in aqueous solutions of organic molecules with carbonyl- and nitrile-containing constituents. In many cases, the vibrational resonances from these moieties shifted toward lower frequency as salt was introduced into solution. This is in contrast to the blue-shift that would be expected based upon Onsager’s reaction field theory. Salts containing well-hydrated cations like Mg2+ or Li+ led to the most pronounced Stark shift for the carbonyl group, while poorly hydrated cations like Cs+ had the greatest impact on nitriles. Moreover, salts containing I– gave rise to larger Stark shifts than those containing Cl–. Molecular dynamics simulations indicated that cations and anions both accumulate around the probe in an ion- and probe-dependent manner. An electric field was generated by the ion pair, which pointed from the cation to the anion through the vibrational chromophore. This resulted from solvent-shared binding of the ions to the probes, consistent with their positions in the Hofmeister series. The “anti-Onsager” Stark shifts occur in both vibrational spectroscopy and fluorescence measurements.
- Published
- 2021
32. Regulation of bone remodeling by vasopressin explains the bone loss in hyponatremia
- Author
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Tamma, Roberto, Li Sun, Cuscito, Concetta, Lu, Ping, Corcelli, Michelangelo, Li, Jianhua, Colaianni, Graziana, Moonga, Surinder S., Di Benedetto, Adriana, Grano, Maria, Colucci, Silvia, Yuen, Tony, New, Maria I., Zallone, Alberta, and Zaidi, Mone
- Published
- 2013
33. Peptide and Peptide-Dependent Motions in MHC Proteins: Immunological Implications and Biophysical Underpinnings
- Author
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Cory M. Ayres, Steven A. Corcelli, and Brian M. Baker
- Subjects
MHC ,peptide ,flexibility ,dynamics ,antigenicity ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Structural biology of peptides presented by class I and class II MHC proteins has transformed immunology, impacting our understanding of fundamental immune mechanisms and allowing researchers to rationalize immunogenicity and design novel vaccines. However, proteins are not static structures as often inferred from crystallographic structures. Their components move and breathe individually and collectively over a range of timescales. Peptides bound within MHC peptide-binding grooves are no exception and their motions have been shown to impact recognition by T cell and other receptors in ways that influence function. Furthermore, peptides tune the motions of MHC proteins themselves, which impacts recognition of peptide/MHC complexes by other proteins. Here, we review the motional properties of peptides in MHC binding grooves and discuss how peptide properties can influence MHC motions. We briefly review theoretical concepts about protein motion and highlight key data that illustrate immunological consequences. We focus primarily on class I systems due to greater availability of data, but segue into class II systems as the concepts and consequences overlap. We suggest that characterization of the dynamic “energy landscapes” of peptide/MHC complexes and the resulting functional consequences is one of the next frontiers in structural immunology.
- Published
- 2017
- Full Text
- View/download PDF
34. Assessing olfactory functions in patients with Barth syndrome.
- Author
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Michele Dibattista, Simona Lobasso, Sebastiano Stramaglia, and Angela Corcelli
- Subjects
Medicine ,Science - Abstract
Barth syndrome is a rare X-linked disease affecting less than 200 individuals worldwide. Several comorbidities have been associated with the pathology and, among those, cardiac myopathy and neutropenia are the most life threatening. The appropriate nutritive support is important to sustain the everyday life of Barth syndrome patients given the chronic fatigue they experience. Since they often prefer salty and fried food, and avoid vegetables and fruits, their eating habit and food preferences do not always provide the proper amount of vitamins and amino acids. It has been indeed reported that Barth syndrome patients have altered taste sensitivity. As olfaction also contributes to food consumption and flavor perception, we decided to investigate their olfactory abilities using the "Sniffin' sticks' extended test". We found no significant difference in any of the tested olfactory abilities between the group of Barth syndrome patients and the healthy controls. In summary, altered food preference of Barth boys could not be easily explained with an altered olfactory perception.
- Published
- 2017
- Full Text
- View/download PDF
35. Lack of effect of adenosine on the function of rodent osteoblasts and osteoclasts in vitro
- Author
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Hajjawi, Mark O. R., Patel, Jessal J., Corcelli, Michelangelo, Arnett, Timothy R., and Orriss, Isabel R.
- Published
- 2016
- Full Text
- View/download PDF
36. A Life Cycle Assessment of Biomethane Production from Waste Feedstock Through Different Upgrading Technologies
- Author
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Ciro Florio, Gabriella Fiorentino, Fabiana Corcelli, Sergio Ulgiati, Stefano Dumontet, Joshua Güsewell, and Ludger Eltrop
- Subjects
life cycle assessment ,biogas upgrading ,cogeneration of electricity and heat from biogas ,environmental assessment of biomethane production ,Technology - Abstract
Upgrading consists of a range of purification processes aimed at increasing the methane content of biogas to reach specifications similar to natural gas. In this perspective, an environmental assessment, based on the Life Cycle Assessment (LCA) method, of different upgrading technologies is helpful to identify the environmental characteristics of biomethane and the critical steps for improvement. The aim of this work is to conduct an LCA of biomethane production from waste feedstock, using the SimaPro software. The study focuses on the comparison of several upgrading technologies (namely, membrane separation, cryogenic separation, pressure swing adsorption, chemical scrubbing, high pressure water scrubbing) and the on-site cogeneration of electricity and heat, including the environmental benefits deriving from the substitution of fossil-based products. The results show a better environmental performance of the cogeneration option in most of the impact categories. The Fossil resource scarcity is the impact category which is mainly benefited by the avoided production of natural gas, with savings of about 0.5 kg oil eq/m3 of biogas for all the investigated technologies, with an average improvement of about 76% compared to conventional cogeneration. The results show that the membrane upgrading technology is slightly more environmentally convenient than the other upgrading technologies.
- Published
- 2019
- Full Text
- View/download PDF
37. PROVIMENTO DA DIREÇÃO ESCOLAR NAS ETECS DO CENTRO PAULA SOUZA: APROXIMAÇÕES SOBRE A GESTÃO DEMOCRÁTICA NA EDUCAÇÃO PROFISSIONAL.
- Author
-
Ferreira Corcelli, Edmeire and Prado Constantino, Paulo Roberto
- Subjects
VOCATIONAL guidance ,VOCATIONAL high schools ,SECONDARY education ,VOCATIONAL education ,VOCATIONAL schools - Abstract
Copyright of Revista Fatec Zona Sul (REFAS) is the property of Revista Fatec Zona Sul (REFAS) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
38. Elucidating the binding mechanism of Hoechst 33258 to a DNA duplex
- Author
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Corcelli, Steven A., primary and Brossard, Erin, additional
- Published
- 2022
- Full Text
- View/download PDF
39. Simulating the binding of a T cell receptor to a peptide-bound major histocompatibility complex
- Author
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Brossard, Erin, primary and Corcelli, Steven A., additional
- Published
- 2022
- Full Text
- View/download PDF
40. DNA minor-groove binder Hoechst 33258 destabilizes base-pairing adjacent to its binding site
- Author
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Andrei Tokmakoff, Shelby L. Brantley, Steven A. Corcelli, and Xin-Xing Zhang
- Subjects
Base pair ,Medicine (miscellaneous) ,Nucleic Acid Denaturation ,010402 general chemistry ,01 natural sciences ,Article ,General Biochemistry, Genetics and Molecular Biology ,Dissociation (chemistry) ,chemistry.chemical_compound ,Biophysical chemistry ,Spectroscopy, Fourier Transform Infrared ,Binding site ,Spectroscopy ,lcsh:QH301-705.5 ,Binding Sites ,010405 organic chemistry ,Ligand ,Hydrogen bond ,DNA Breaks ,Hydrogen Bonding ,DNA ,0104 chemical sciences ,Thymine ,Crystallography ,chemistry ,lcsh:Biology (General) ,Bisbenzimidazole ,Thermodynamics ,General Agricultural and Biological Sciences - Abstract
Understanding the dynamic interactions of ligands to DNA is important in DNA-based nanotechnologies. By structurally tracking the dissociation of Hoechst 33258-bound DNA (d(CGCAAATTTGCG)2) complex (H-DNA) with T-jump 2D-IR spectroscopy, the ligand is found to strongly disturb the stability of the three C:G base pairs adjacent to A:T the binding site, with the broken base pairs being more than triple at 100 ns. The strong stabilization effect of the ligand on DNA duplex makes this observation quite striking, which dramatically increases the melting temperature and dissociation time. MD simulations demonstrate an important role of hydration water and counter cations in maintaining the separation of terminal base pairs. The hydrogen bonds between the ligand and thymine carbonyls are crucial in stabilizing H-DNA, whose breaking signal appearing prior to the complete dissociation. Thermodynamic analysis informs us that H-DNA association is a concerted process, where H cooperates with DNA single strands in forming H-DNA., Using T-jump 2D-IR spectroscopy, Zhang et al. show that DNA minor-groove binder Hoechst 33258 strongly destabilizes its complex with DNA (H-DNA). This study suggests that the association of H-DNA is a concerted process, where Hoechst 33258 cooperates with DNA single strands in forming H-DNA.
- Published
- 2020
41. Hydrogen Bond Exchange and Ca2+ Binding of Aqueous N-Methylacetamide Revealed by 2DIR Spectroscopy
- Author
-
Danielle K. Geremia, Olivia M. Cracchiolo, Steven A. Corcelli, and Arnaldo L. Serrano
- Subjects
Aqueous solution ,010304 chemical physics ,Infrared ,Chemistry ,Hydrogen bond ,Astrophysics::Cosmology and Extragalactic Astrophysics ,010402 general chemistry ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,Crystallography ,Molecular dynamics ,Mathematics::Category Theory ,0103 physical sciences ,N-methylacetamide ,Materials Chemistry ,Astrophysics::Solar and Stellar Astrophysics ,Physical and Theoretical Chemistry ,Ca2 binding ,Spectroscopy ,Astrophysics::Galaxy Astrophysics - Abstract
Cation effects on proteins have been a challenge to understand. Herein, we present two-dimensional infrared (2DIR) spectroscopic measurements, coupled with molecular dynamics and spectroscopic calc...
- Published
- 2020
42. Lipidomics of intact mitochondria by MALDI-TOF/MS
- Author
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Roberto Angelini, Rita Vitale, Vinay A. Patil, Tiziana Cocco, Bernd Ludwig, Miriam L. Greenberg, and Angela Corcelli
- Subjects
cardiolipin ,heart ,Paracoccus denitrificans ,yeast ,9-aminoacridine ,matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ,Biochemistry ,QD415-436 - Abstract
A simple and fast method of lipid analysis of isolated intact mitochondria by means of MALDI-TOF mass spectrometry is described. Mitochondria isolated from bovine heart and yeast have been employed to set up and validate the new method of lipid analysis. The mitochondrial suspension is directly applied over the target and, after drying, covered by a thin layer of the 9-aminoacridine matrix solution. The lipid profiles acquired with this procedure contain all peaks previously obtained by analyzing the lipid extracts of isolated mitochondria by TLC and/or mass spectrometry. The novel procedure allows the quick, simple, precise, and accurate analysis of membrane lipids, utilizing only a tiny amount of isolated organelle; it has also been tested with intact membranes of the bacterium Paracoccus denitrificans for its evolutionary link to present-day mitochondria. The method is of general validity for the lipid analysis of other cell fractions and isolated organelles.
- Published
- 2012
- Full Text
- View/download PDF
43. Allergen risk: how to manage it? What food industries could make and which methods can be used to control the products and the processes.
- Author
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N. Iseppi, G. Corcelli, T. Malisano, G. Rossetti, and Elisabetta Genta
- Subjects
Allergen ,Meat ,HACCP ,Food. ,Food processing and manufacture ,TP368-456 - Abstract
For food companies manage the risk of allergens in their processes has become increasingly important, both to fulfill the laws requirements and for all the criteria of customers and consumers more aware and sensitive about this matter. The experience of this practical contribution is related to meat processing companies such as producers of meat preparations and meat products (products of salumeria). The goal is to explain what are the approaches used to manage the risk, what are the pratical and analytical methods designed to control and verify the right management related to cross contamination, and provide some data on the checks carried out in self-control plans.
- Published
- 2012
- Full Text
- View/download PDF
44. Correction to Vibrational Spectroscopic Map, Vibrational Spectroscopy, and Intermolecular Interaction
- Author
-
Bartosz Błasiak, Jens Bredenbeck, Carlos R. Baiz, Jun Ho Choi, Lauren J. Webb, Jonathan D. Hirst, Gerhard Stock, Andrei Tokmakoff, Lu Wang, Keisuke Tominaga, Nien-Hui Ge, Kijeong Kwac, Mike Reppert, Hiroaki Maekawa, Hajime Torii, John E. Straub, James L. Skinner, Shinji Saito, Steven A. Corcelli, Thomas L. C. Jansen, Martin T. Zanni, Chi-Jui Feng, Sean Garrett-Roe, Megan C. Thielges, Casey H. Londergan, Arend G. Dijkstra, Minhaeng Cho, Santanu Roy, Kevin J. Kubarych, and Magnus W. D. Hanson-Heine
- Subjects
Crystallography ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Intermolecular interaction ,Chemistry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Chemical Sciences ,Infrared spectroscopy ,General Chemistry - Published
- 2021
45. MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine
- Author
-
Roberto Angelini, Francesco Babudri, Simona Lobasso, and Angela Corcelli
- Subjects
glycerolipids (diphytanylglycerol ether analogs) ,glycerophospholipids (diphytanylglycerolphosphate ether analogs) ,Halobacterium salinarum ,purple membrane ,matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ,Biochemistry ,QD415-436 - Abstract
A method of direct lipid analysis by MALDI mass spectrometry in intact membranes, without prior extraction/separation steps, is described. The purple membrane isolated from the extremely halophilic archaeon Halobacterium salinarum was selected as model membrane. Lyophilized purple membrane were grinded with 9-aminoacridine (9-AA) as dry matrix, and the powder mixture was crushed in a mechanical die press to form a thin pellet. Small pieces of the pellet were then attached to the MALDI target and directly analyzed. In parallel, individual archaebacterial phospholipids and glycolipids, together with the total lipid extract of the purple membrane, were analyzed by MALDI-TOF/MS using 9-AA as the matrix in solution. Results show that 9-AA represents a suitable matrix for the conventional MALDI-TOF/MS analysis of lipid extracts from archaeal microorganisms, as well as for fast and reliable direct dry lipid analysis of lyophilized archaebacterial membranes. This method might be of general application, offering the advantage of quickly gaining information about lipid components without disrupting or altering the membrane matrix.
- Published
- 2010
- Full Text
- View/download PDF
46. The acylhalocapnines of halophilic bacteria: structural details of unusual sulfonate sphingoids
- Author
-
Maristella Baronio, Veronica M.T. Lattanzio, Natalie Vaisman, Aharon Oren, and Angela Corcelli
- Subjects
sulfonolipids ,capnine ,halocapnine ,Bacteroidetes ,Biochemistry ,QD415-436 - Abstract
Sulfonate sphingoids or sulfonolipids are bioactive unusual compounds found in members of the Bacteroidetes family. The present report describes the structures of sulfonolipids of halophilic bacteria, sharing structural similarity with compounds of fungal origin inhibiting the serine palmitoyl transferase and with capnines, known as antagonists of von Willebrandt factor. Two sulfonolipids (SL1 and SL2) were isolated from the lipid extract of the halophile Salisaeta longa and analyzed by ESI-MS/MS. SL1 and SL2 structures have in common the long chain aminosulfonate 2-carboxy-2-amino-3,4-hydroxy-17 methyloctadec-5-ene-1-sulfonic for which the common name of halocapnine is suggested. The hydroxyl group on carbon 3 of aminosulfonate moiety is acylated: iso C15 and iso hydroxy C15 chains are present in SL1 and SL2, respectively. The levels of the two different sulfonolipids in the bacterium were found to be modulated by the proportion of sodium and magnesium ions in the environment.
- Published
- 2010
- Full Text
- View/download PDF
47. IL-17 Expression by Tubular Epithelial Cells in Renal Transplant Recipients with Acute Antibody-Mediated Rejection
- Author
-
Loverre, A., Tataranni, T., Castellano, G., Divella, C., Battaglia, M., Ditonno, P., Corcelli, M., Mangino, M., Gesualdo, L., Schena, F.P., and Grandaliano, G.
- Published
- 2011
- Full Text
- View/download PDF
48. Cardiolipin increases in chromatophores isolated from Rhodobacter sphaeroides after osmotic stress: structural and functional roles
- Author
-
Vincenzo De Leo, Lucia Catucci, Andrea Ventrella, Francesco Milano, Angela Agostiano, and Angela Corcelli
- Subjects
lipid-protein interactions ,reaction center ,thermal denaturation ,photoxidative damage ,flash photolysis ,Biochemistry ,QD415-436 - Abstract
Chromatophores isolated from cells of Rhodobacter sphaeroides exposed to hypertonic solutions were enriched in cardiolipin (CL). Because CL levels are raised by increasing the incubation time of R. sphaeroides in hypertonic solutions, it was possible to isolate chromatophores containing different CL amounts by starting from cells incubated in hypertonic solutions for different times. The functionality and stability of the photosynthetic proteins in chromatophore membranes having different CL levels were investigated. Reaction center (RC) stabilization with respect to thermal denaturation and photoxidative damage was observed by flash photolysis and fluorescence emission experiments in CL-enriched chromatophores. To gain detailed information about the structures of endogenous CLs, this lipid family was isolated and purified by preparative TLC, and characterized by high-resolution mass spectrometry. We conclude that osmotic shock can be used as a tool to modulate CL levels in isolated chromatophores and to change the composition of the RC lipid annulus, avoiding membrane artifacts introduced by the use of detergents.
- Published
- 2009
- Full Text
- View/download PDF
49. A Lipidomic Approach to Identify Potential Biomarkers in Exosomes From Melanoma Cells With Different Metastatic Potential
- Author
-
Lobasso, Simona, primary, Tanzarella, Paola, additional, Mannavola, Francesco, additional, Tucci, Marco, additional, Silvestris, Francesco, additional, Felici, Claudia, additional, Ingrosso, Chiara, additional, Corcelli, Angela, additional, and Lopalco, Patrizia, additional
- Published
- 2021
- Full Text
- View/download PDF
50. Lipids of the ultra-thin square halophilic archaeon Haloquadratum walsbyi
- Author
-
Simona LoBasso, Patrizia LoPalco, Giuseppe Mascolo, and Angela Corcelli
- Subjects
Microbiology ,QR1-502 - Abstract
The lipid composition of the extremely halophilic archaeon Haloquadratum walsbyi was investigated by thin-layer chromatography and electrospray ionization-mass spectrometry. The analysis of neutral lipids showed the presence of vitamin MK-8, squalene, carotene, bacterioruberin and several retinal isomers. The major polar lipids were phosphatidylglycerophosphate methyl ester, phosphatidylglycerosulfate, phosphatidylglycerol and sulfated diglycosyl diether lipid. Among cardiolipins, the tetra-phytanyl or dimeric phospholipids, only traces of bisphosphatidylglycerol were detected. When the cells were exposed to hypotonic medium, no changes in the membrane lipid composition occurred. Distinguishing it from other extreme halophiles of the Halobacteriaceae family, the osmotic stress did not induce the neo-synthesis of cardiolipins in H. walsbyi. The difference may depend on the three-laminar structure of the cell wall, which differs significantly from that of other Haloarchaea.
- Published
- 2008
- Full Text
- View/download PDF
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