123 results on '"Christie V"'
Search Results
2. Pippi: Practical Protocol Instantiation
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Christie V, Samuel H., Chopra, Amit K., and Singh, Munindar P.
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Computer Science - Multiagent Systems - Abstract
A protocol specifies interactions between roles, which together constitute a multiagent system (MAS). Enacting a protocol presupposes that agents are bound to the its roles. Existing protocol-based approaches, however, do not adequately treat the practical aspects of how roles bindings come about. Pippi addresses this problem of MAS instantiation. It proposes the notion of a metaprotocol, enacting which instantiates a MAS suitable for enacting a given protocol. Pippi demonstrates the subtleties involved in instantiating MAS arising from protocol composition, correlation, and decentralization. To address these subtleties and further support practical application patterns, we introduce an enhanced protocol language, with support for parameter types (including role and protocol typed parameters, for metaprotocols), interface flexibility, and binding constraints. We discuss the realization of our approach through an extended agent architecture, including the novel concept of a MAS adapter for contact management. We evaluate Pippi's expressiveness by demonstrating common patterns for agent discovery., Comment: 8 pages, to be published in Proc. of the 21st International Conference on Autonomous Agents and Multiagent Systems (AAMAS 2022), Online
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- 2022
3. Coreografías
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Jacques Derrida, Christie V. McDonald, Joana Masó, and Javier Bassas Vila (trad.)
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Jacques Derrida ,filosofía ,diferancia ,género ,feminismos ,Women. Feminism ,HQ1101-2030.7 - Abstract
Traducción al castellano del intercambio de correspondencia entre Jacques Derrida y Christie V. McDonald, en el otoño de 1981. A partir de las preguntas de McDonald, el filósofo reflexiona en torno a temas como el género, la diferencia sexual, los movimientos feministas, los feminismos, el lugar de la mujer, y el aporte que una perspectiva como la deconstrucción ofrece a su abordaje.
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- 2010
4. Hercule: Representing and Reasoning about Norms as a Foundation for Declarative Contracts over Blockchain
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Christie V, Samuel H., Chopra, Amit K., and Singh, Munindar P.
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Computer Science - Multiagent Systems - Abstract
Current blockchain approaches for business contracts are based on smart contracts, namely, software programs placed on a blockchain that are automatically executed to realize a contract. However, smart contracts lack flexibility and interfere with the autonomy of the parties concerned. We propose Hercule, an approach for declaratively specifying blockchain applications in a manner that reflects business contracts. Hercule represents a contract via regulatory norms that capture the involved parties' expectations of one another. It computes the states of norms (hence, of contracts) from events in the blockchain. Hercule's novelty and significance lie in that it operationalizes declarative contracts over semistructured databases, the underlying representation for practical blockchain such as Hyperledger Fabric and Ethereum. Specifically, it exploits the map-reduce capabilities of such stores to compute norm states. We demonstrate that our implementation over Hyperledger Fabric can process thousands of events per second, sufficient for many applications.
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- 2021
5. An Evaluation of Communication Protocol Languages for Engineering Multiagent Systems
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Chopra, Amit K., Christie V, Samuel H., and Singh, Munindar P.
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Computer Science - Software Engineering ,D.3.0 ,D.2.11 ,I.2.11 - Abstract
Communication protocols are central to engineering decentralized multiagent systems. Modern protocol languages are typically formal and address aspects of decentralization, such as asynchrony. However, modern languages differ in important ways in their basic abstractions and operational assumptions. This diversity makes a comparative evaluation of protocol languages a challenging task. We contribute a rich evaluation of modern protocol languages based on diverse approaches. Among the selected languages, Scribble is based on session types; Trace-C and Trace-F on trace expressions; HAPN on hierarchical state machines, and BSPL on information causality. Our contribution is four-fold. One, we contribute important criteria for evaluating protocol languages. Two, for each criterion, we compare the languages on the basis of whether they are able to specify elementary protocols that go to the heart of the criterion. Three, for each language, we map our findings to a canonical architecture style for multiagent systems, highlighting where the languages depart from the architecture. Four, we identify a few design principles for protocol languages as guidance for future research.
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- 2019
6. Mandrake: multiagent systems as a basis for programming fault-tolerant decentralized applications
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Christie, V, Samuel H., Chopra, Amit K., and Singh, Munindar P.
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- 2022
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7. “Coreografias” : entrevista com Jacques Derrida
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Derrida, Jacques, McDonald, Christie V., Rodrigues, Carla, and Grenha, Tatiana
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- 2019
8. Pippi: Practical Protocol Instantiation
- Author
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H. Christie V, Samuel H., Chopra, Amit K., Singh, Munindar P., H. Christie V, Samuel H., Chopra, Amit K., and Singh, Munindar P.
- Abstract
A protocol specifies interactions between roles, which together constitute a multiagent system (MAS). Enacting a protocol presupposes that agents are bound to the its roles. Existing protocol-based approaches, however, do not adequately treat the practical aspects of how roles bindings come about. Pippi addresses this problem of MAS instantiation. It proposes the notion of a metaprotocol, enacting which instantiates a MAS suitable for enacting a given protocol. Pippi demonstrates the subtleties involved in instantiating MAS arising from protocol composition, correlation, and decentralization. To address these subtleties and further support practical application patterns, we introduce an enhanced protocol language, with support for parameter types (including role and protocol typed parameters, for metaprotocols), interface flexibility, and binding constraints. We discuss the realization of our approach through an extended agent architecture, including the novel concept of a MAS adapter for contact management. We evaluate Pippi's expressiveness by demonstrating common patterns for agent discovery.
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- 2022
9. 'Choreographies': Interview with Jacques Derrida/'Coreografias': entrevista com Jacques Derrida
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McDonald, Christie V., Rodrigues, Carla, and Grenha, Tatiana
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- 2019
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10. Structure of the Cooperative Xis: DNA Complex Reveals a Micronucleoprotein Filament That Regulates Phage Lambda Intasome Assembly
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Abbani, Mohamad A., Papagiannis, Christie V., Sam, My D., Cascio, Duilio, Johnson, Reid C., and Clubb, Robert T.
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- 2007
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11. The Reading and Writing of Utopia in Denis Diderot's "Supplément au voyage de Bougainville"
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McDonald, Christie V.
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- 1976
12. Interview: Choreographies: Jacques Derrida and Christie V. McDonald
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McDonald, Christie V. and Derrida, Jacques
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- 1982
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13. The Model of Reading in Rousseau's Dialogues
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McDonald, Christie V.
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- 1978
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14. Fractured Readers
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McDonald, Christie V.
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- 1982
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15. The Sophistry of Heuristics
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McDonald, Christie V.
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- 1985
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16. Literary History: Interpretation inside out?
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McDonald, Christie V.
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- 1981
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17. Coreografías
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Derrida, Jacques and McDonald, Christie V.
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Jacques Derrida ,Feminismes ,Diferancia ,Filosofía ,Género ,Filosofia ,Gènere ,Feminismos - Abstract
Traducción al castellano del intercambio de correspondencia entre Jacques Derrida y Christie V. McDonald, en el otoño de 1981. A partir de las preguntas de McDonald, el filósofo reflexiona en torno a temas como el género, la diferencia sexual, los movimientos feministas, los feminismos, el lugar de la mujer, y el aporte que una perspectiva como la deconstrucción ofrece a su abordaje.
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- 2021
18. Pippi: Practical Protocol Instantiation
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H. Christie V, Samuel H., Chopra, Amit K., Singh, Munindar P., H. Christie V, Samuel H., Chopra, Amit K., and Singh, Munindar P.
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- 2021
19. Deserv:Decentralized Serverless Computing
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Christie V, Samuel H., Chopra, Amit K., Singh, Munindar P., Christie V, Samuel H., Chopra, Amit K., and Singh, Munindar P.
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A decentralized application involves multiple autonomous principals, e.g., humans and organizations. Autonomy motivates (i) specifying a decentralized application via a protocol that captures the interactions between the principals, and (ii) a programming model that enables each principal to independently (from other principals) construct its own protocol-compliant agent. An agent encodes its principal's decision making and represents it in the application. We contribute Deserv, the first protocol-based programming model for decentralized applications that is suited to the cloud. Specifically, Deserv demonstrates how to leverage function-as-a-service (FaaS), a popular serverless programming model, to implement agents. A notable feature of Deserv is the use declarative protocols to specify interactions. Declarative protocols support implementing stateful agents in a manner that naturally exploits the concurrency and autoscaling benefits offered by serverless computing.
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- 2021
20. Deserv : Decentralized Serverless Computing
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Christie V, Samuel H., Chopra, Amit K., Singh, Munindar P., Christie V, Samuel H., Chopra, Amit K., and Singh, Munindar P.
- Abstract
A decentralized application involves multiple autonomous principals, e.g., humans and organizations. Autonomy motivates (i) specifying a decentralized application via a protocol that captures the interactions between the principals, and (ii) a programming model that enables each principal to independently (from other principals) construct its own protocol-compliant agent. An agent encodes its principal's decision making and represents it in the application. We contribute Deserv, the first protocol-based programming model for decentralized applications that is suited to the cloud. Specifically, Deserv demonstrates how to leverage function-as-a-service (FaaS), a popular serverless programming model, to implement agents. A notable feature of Deserv is the use declarative protocols to specify interactions. Declarative protocols support implementing stateful agents in a manner that naturally exploits the concurrency and autoscaling benefits offered by serverless computing.
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- 2021
21. 2042P Improving breast cancer outcomes for indigenous women
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Christie, V., Green, D., Riley, L., and Gwynne, K.
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- 2023
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22. A Dominant Suppressor Mutation of the met30 Cell Cycle Defect Suggests Regulation of the Saccharomyces cerevisiae Met4-Cbf1 Transcription Complex by Met32
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Su, Ning-Yuan, Ouni, Ikram, Papagiannis, Christie V., and Kaiser, Peter
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- 2008
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23. Bungie: Improving Fault Tolerance via Extensible Application-Level Protocols
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Christie V, Samuel H, primary, Chopra, Amit Khushwant, additional, and Singh, Munindar P., additional
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- 2021
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24. Inverted slot-mode slow-wave structures for traveling-wave tubes
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Christie, V. Latha, Kumar, Lalit, and Balakrishnan, N.
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Computer-generated environments -- Usage ,Computer simulation -- Usage ,Dispersion -- Analysis ,Wave frequency -- Analysis ,Plasma physics -- Analysis ,Business ,Computers ,Electronics ,Electronics and electrical industries - Abstract
The dispersion and impedance characteristics of an inverted slot-mode (ISM) slow-wave structure computed by three different techniques, i.e., an analytical model based on a periodic quasi-TEM approach, an equivalent-circuit model, and 3-D electromagnetic simulation are obtained and compared. The comparison was carried out for three different slot-mode structures at S-, C-, and X-bands. The approach was also validated with experimental measurements on a practical X-band ISM traveling-wave tube. The design of ferruleless ISM slow-wave structures, both in circular and rectangular formats, has also been proposed and the predicted dispersion characteristics for these two geometries are compared with 3-D simulation and cold-test measurements. The impedance characteristics for all three designs are also compared. Index Terms--Dispersion, equivalent circuits, measurement, simulation, slow-wave structures, traveling-wave tubes (TWTs).
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- 2007
25. An Evaluation of Communication Protocol Languages for Engineering Multiagent Systems
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Chopra, Amit K, primary, Christie V, Samuel H, additional, and Singh, Munindar P., additional
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- 2020
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26. Protocols Over Things: A Decentralized Programming Model for the Internet of Things
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Christie V, Samuel H., primary, Smirnova, Daria, additional, Chopra, Amit K., additional, and Singh, Munindar P., additional
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- 2020
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27. Allogeneic Serum Eye Drops: A Randomized Clinical Trial to Evaluate the Clinical Effectiveness of Two Drop Sizes
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Christie Vermeulen, Lars L. J. van der Burg, Nan van Geloven, Catharina A. Eggink, Yanny Y. Y. Cheng, Rudy M. M. A. Nuijts, Robert P. L. Wisse, Chantal M. van Luijk, Carla Nieuwendaal, Lies Remeijer, Pieter F. van der Meer, Dirk de Korte, and Thomas R. L. Klei
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Allogeneic serum eye drops ,Application system ,Drop size ,Dry eye disease (DED) ,Naïve patients with DED ,Ocular Surface Disease Index (OSDI) ,Ophthalmology ,RE1-994 - Abstract
Abstract Introduction Allogeneic serum from blood donors is starting to be used to treat patients with dry eye disease (DED). However, the optimal dose is not known. We therefore aimed to evaluate the clinical efficaciousness and user-friendliness of micro-sized versus conventional-sized allogeneic serum eye drops (SEDs). Methods In a randomized trial, patients with DED first receive micro-sized SEDs (7 µl/unit) for 1 month, followed by a 1-month washout, before receiving conventional-sized SEDs (50 µl/unit) for 1 month; or vice versa. The primary endpoint was the Ocular Surface Disease Index (OSDI) score. Secondary endpoints were tear break-up time (TBT), tear production (TP), and presence of corneal punctate lesions (CP). The user-friendliness of both application systems was also compared. A linear mixed model for cross-over design was applied to compare both treatments. Results Forty-nine patients completed the trial. The mean OSDI score significantly improved from 52 ± 3 to 41 ± 3 for micro-sized SEDs, and from 54 ± 3 to 45 ± 3 for conventional-sized SEDs. Non-inferiority (margin = 6) of micro-sized SEDs was established. We demonstrate a significant improvement for TBT in case of conventional-sized SEDs and for CP in both treatment groups. TP trended towards an improvement in both treatment groups. The user-friendliness of the conventional drop system was significantly higher. Conclusions For the first time, non-inferiority of micro-sized allogeneic SEDs was established. The beneficial effect of both SED volumes was similar as measured by the OSDI score. Although user-friendliness of the micro drop system was significantly lower, it is an attractive alternative as it saves valuable donor serum. Trial Registration ClinicalTrials.gov (NCT03539159).
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- 2023
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28. Sensitivity to Neutralizing Antibodies and Resistance to Type I Interferons in SARS-CoV-2 R.1 Lineage Variants, Canada
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Rajesh Abraham Jacob, Ali Zhang, Hannah O. Ajoge, Michael R. D'Agostino, Kuganya Nirmalarajah, Altynay Shigayeva, Wael L. Demian, Sheridan J.C. Baker, Hooman Derakhshani, Laura Rossi, Jalees A. Nasir, Emily M. Panousis, Ahmed N. Draia, Christie Vermeiren, Jodi Gilchrist, Nicole Smieja, David Bulir, Marek Smieja, Michael G. Surette, Andrew G. McArthur, Allison J. McGeer, Samira Mubareka, Arinjay Banerjee, Matthew S. Miller, and Karen Mossman
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COVID-19 ,SARS-CoV-2 ,coronavirus disease ,severe acute respiratory syndrome coronavirus 2 ,viruses ,respiratory infections ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Isolating and characterizing emerging SARS-CoV-2 variants is key to understanding virus pathogenesis. In this study, we isolated samples of the SARS-CoV-2 R.1 lineage, categorized as a variant under monitoring by the World Health Organization, and evaluated their sensitivity to neutralizing antibodies and type I interferons. We used convalescent serum samples from persons in Canada infected either with ancestral virus (wave 1) or the B.1.1.7 (Alpha) variant of concern (wave 3) for testing neutralization sensitivity. The R.1 isolates were potently neutralized by both the wave 1 and wave 3 convalescent serum samples, unlike the B.1.351 (Beta) variant of concern. Of note, the R.1 variant was significantly more resistant to type I interferons (IFN-α/β) than was the ancestral isolate. Our study demonstrates that the R.1 variant retained sensitivity to neutralizing antibodies but evolved resistance to type I interferons. This critical driving force will influence the trajectory of the pandemic.
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- 2023
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29. Utility of hybrid whole genome sequencing in assessing potential nosocomial VIM transmission
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David Burke James Mahoney, Xena Li, Patryk Aftanas, Christie Vermeiren, Robert Kozak, Kevin Katz, and Finlay Maguire
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Infectious and parasitic diseases ,RC109-216 ,Public aspects of medicine ,RA1-1270 - Abstract
Hybrid whole genome sequencing was used to investigate if nosocomial Verona integron-encoded metallo-β-lactamase (VIM) carbapenemase transmission occurred between two patients without epidemiological links or common pathogens. Challenges in genomic methodology and appropriate analytical depth for mobile carbapenemase outbreaks are described including how inappropriate choices can mislead results and impact infection control practices.
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- 2024
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30. “Coreografias”: entrevista com Jacques Derrida
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McDonald, Christie V., primary, Rodrigues, Carla, additional, and Grenha, Tatiana, additional
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- 2019
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31. Leadership behaviours in interprofessional student teamwork
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Christie van Diggele, Chris Roberts, and Stuart Lane
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Interprofessional ,Leadership ,Teamwork ,Health professions ,Education ,Peer review ,Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract Background Effective leaders support high-quality patient care and improve patient safety by embodying a collective leadership style. Training in leadership skills needs to be integrated longitudinally throughout a clinician’s career. Models of leadership drawn from organisational theories can provide a conceptual framework for cultivating student leadership qualities during teamwork and the evaluation of emergent outcomes. Using the conceptual framework of Situational Leadership Theory, we sought to explore the leadership qualities identified by students of their team members, during a large scale interprofessional learning activity. Methods In 2018, 1674 students from 11 health disciplines were required to participate in the “Health Collaboration Challenge” (HCC). The HCC required students to work in small interprofessional teams of five or six students. Following team activities, students were required to provide constructive written feedback to their team members. Peer feedback data were coded and categorised into themes using the conceptual framework of Situational Leadership Theory. Data were then quantified within each theme. Results A total of 1282 comments were analysed. The most frequent comments related to ‘delegating’ (456/1282, 36%) and ‘supporting’ (402/1282, 31%). This was followed by comments categorised as ‘directing’ (244/1282, 19%), and ‘coaching’ (180/1282, 14%) leadership styles. Notably, a total of 1112/2597 (43%) of comments were unconstructive. A total of 298 comments provided by students informed their peers of areas for self-improvement. The most frequent comments were recommendations relating to ‘active team member contribution’ (111/298; 37%), followed by ‘communication’ (83/298; 28%), ‘interprofessional practice’ (77/298; 26%), and ‘disciplinary knowledge’ (27/298; 9%). Conclusion Although most students demonstrated a reasonable ability to display leadership behaviours appropriate to teamwork, further development is needed through training. Leadership skills are an expectation of health professional graduates, and should be explicitly taught and vertically integrated within interprofessional education curricula. Further research is warranted in how students contribute to and understand the requirements of leadership within interprofessional teams.
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- 2022
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32. Student Interprofessional Facilitator Training (SIFT) program: building capacity in clinical education leadership
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Christie van Diggele, Stuart Lane, and Chris Roberts
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Interprofessional education ,Leadership ,Health professional ,Teacher training ,Peer assisted learning ,Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract Background While there are many teacher training programs for health professional students, few are interprofessional, and few integrate assessment and feedback prior to participation as peer teachers. In 2021, The Student Interprofessional Facilitator Training (SIFT) program was developed to allow senior students, already trained in peer teaching, to revise, build on, and practice their newly acquired skills in an interprofessional context. The aim of this study was to explore participant perception and performance, and the contextual factors that influence student aspirations as clinical teachers. Methods Alumni of the 2021 Peer Teacher Training program (n = 74) were invited to participate in the SIFT program. Those who participated were invited to attend individual semi-structured interviews. Thematic analysis was used to code and categorise data into themes, using Communities of Practice as a conceptual framework. Skills in interprofessional facilitation were observed, assessed and students were provided with individual feedback. Assessment data were analysed using descriptive statistics. Results Sixteen students from six disciplines joined the SIFT program, and 13/16 (81%) completed. Students were from medicine, nursing, diagnostic radiography, medical imaging, dentistry and speech pathology. Students reported an increased recognition of teaching as a learned skill, development of clinician identity formation as educators, development of interprofessional communication skills, increased awareness of the roles of other health professions, and an increased understanding of leadership. Participants expressed a desire for additional opportunities for interprofessional networking and peer teaching. A good level of competence in facilitation skills was reached by participants. Conclusion The SIFT program provided a sustainable framework for health professional students to develop and evidence their teaching and leadership skills in an interprofessional context. This study highlighted the important role of observation, assessment and feedback in student teacher training programs. The process of clear assessment guidelines, direct observation with feedback from supervisors provided a way to ensure quality improvement in peer teaching. The SIFT program will help to build capacity of interprofessional programs where large numbers of teachers are required for small group teaching. The next step will be to ensure a variety of opportunities within interprofessional contexts, and with face-to-face engagement.
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- 2022
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33. THE UTOPIA OF THE TEXT: DIDEROT'S "ENCYCLOPEDIE"
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McDonald, Christie V.
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- 1980
34. JACQUES DERRIDA'S READING OF ROUSSEAU
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McDonald, Christie V.
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- 1979
35. S187: IMMUNOLOGICAL AGING HAS A NEGATIVE IMPACT ON CLINICAL OUTCOME IN ELDERLY MULTIPLE MYELOMA PATIENTS
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Wassilis Bruins, Carolien Duetz, Kaz Groen, Charlotte Korst, Vera de Jonge, Christie Verkleij, Rosa Rentenaar, Meliha Cosovic, Niels W.C.J. van de Donk, Sonja Zweegman, and Tuna Mutis
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Diseases of the blood and blood-forming organs ,RC633-647.5 - Published
- 2023
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36. P1506: TECLISTAMAB REDUCES POLYCLONAL IMMUNOGLOBULIN LEVELS AND IMPAIRS VACCINATION RESPONSES IN HEAVILY PRETREATED MM PATIENTS
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Kristine Frerichs, Christie Verkleij, Maria-Victoria Mateos, Sonja Zweegman, Kaz Groen, Ilse Kuipers, Tom Martin, Cesar Rodriguez, Ajay Nooka, Sandy Kruijswijk, Sheri Skerget, Margaret Doyle, Arnob Banerjee, Katherine Chastain, Alfredo Perales-Puchalt, Tara Stephenson, Clarissa Uhlar, Rachel Kobos, Raluca Verona, and Niels W.C.J. van de Donk
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Diseases of the blood and blood-forming organs ,RC633-647.5 - Published
- 2023
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37. High PrEP uptake, adherence, persistence and effectiveness outcomes among young Thai men and transgender women who sell sex in Bangkok and Pattaya, Thailand: findings from the open-label combination HIV prevention effectiveness (COPE) studyResearch in context
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Brian W. Weir, Andrea L. Wirtz, Tareerat Chemnasiri, Stefan D. Baral, Michele Decker, Chen Dun, Sandra Hsu Hnin Mon, Chaiwat Ungsedhapand, Eileen F. Dunne, Joseph Woodring, Sarika Pattanasin, Wichuda Sukwicha, Michael C. Thigpen, Anchalee Varangrat, Anchalee Warapornmongkholkul, Siobhan O'Connor, Julie P. Ngo, Noor Qaragholi, Haley I. Sisel, Jasmine M. Truong, Surang Janyam, Danai Linjongrat, Somchai Sriplienchan, Pachara Sirivongrangson, James F. Rooney, Patrick Sullivan, Boosbun Chua-Intra, Andrew C. Hickey, Chris Beyrer, Andrea Wirtz, Brian Weir, Stefan Baral, James Case, Jasmine Truong, Julie Ngo, Haley Sisel, Anupong Chitwarakorn, Wasin Matsee, Pratakpong Wongkiti, Chidanan Krasan, Anchana Chainuwong, Nauwarat Imlimtharn, Potcharawan Reansoi, Teeraparp Watanatanyaporn, Jarupa Nuamlert, Supannikar Namwong, Jutarat Phetnark, Wachirawit Supasa, Siriporn Sueayot, Andrew Hickey, Michael Thigpen, Eileen Dunne, Christie Vu, Patrick Flaherty, Timothy Holtz, Anekpong Chanthaweesirirat, Warunee Thienkrua, Pitthaya Disprayoon, Kanjana Kamkong, Dararat Worrajittanon, Supawadee Na-Pompet, Chonlanot Sariwatta, Patnaree Oungprasertgul, Phanurassamee Sittidech, Jirawat Suksamosorn, Kesinee Sujina, Wannee Chonwattana, Nichnawee Kamchaithep, Nongkran Tatakham, Pikunchai Luechai, Philip Mock, Betsy Cadwell, Ram Shrestha, Baranee Balmongkol, Boonyos Raengsakulrach, Wanna Leelawiwat, Wanna Suwannaphan, Achara Sriinsut, Punneeporn Wasinrapee, Pornchanok Chanathalay, Nutthawoot Promda, Santi Winaitham, Oranuch Kongpechsatit, Kusuma Auethavornanan, Jaray Tongtoyai, Pairote Tararut, Atitaya McNamara, Famui Mueanpai, Natthaga Sakulploy, Kanokpan Pancharoen, Chariya Utenpitak, Caroline Fukuda, Thitima Cherdtrakulkiat, Tanyawarin Janthiraj, Anuwat Sriporn, Natee Prathummart, Patsaraporn Khongsom, Navakarn Navanuch, Rinda Wongbenchaporn, Chanya Peerapatdit, Pechpailin Khlaimanee, Patcharat Niyamakom, Narongritt Tippanont, Somsak Yafant, Tatchai Ruanpang, Siripak Pongthai, Kamolnetr Okanurak, Aronrag Meeyai, Phubet Panpet, Orawan Fungfoosri, Prisana Boonyawan, Theeranat Sangprasert, Natthawirojn Inthanin, Teppanan Sangiamjit, Somporn Saiwaew, Konlawat Pawong, Chamrong Phaengnongyang, Atachai Phunkron, Denchai Srikrongthong, Thanaphat Dokrak, Phathranis Meekrua, Saman Sumalu, Cawee Kanlose, Prasopsuk Thapwong, Kritsanapol Kaewboonta, Pornpichit Brutrat, Waris Watthanayeam, Apichat Udomjirasirichot, Midnight Poonkasetwattana, Silapakhon Kongsakul, Michael Badorrek, Andrey Tran, Ryan Figueiredo, Safir Soeparna, Wattana Keiangpa, Apiwit Tibamrung, Sunadda Samana, Hidayah Syahputra, Worapon Rattanawarawong, Rachel Valencia, Usha Sharma, Adeola Adeyeye, James Rooney, Pojjana Hunchangsith, Tanyaporn Wansom, Thomas Guadamuz, and Annette Sohn
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HIV ,Prevention ,Pre-exposure prophylaxis ,Men who have sex with men ,Transgender persons ,Sex work ,Public aspects of medicine ,RA1-1270 - Abstract
Summary: Background: Daily oral pre-exposure prophylaxis (PrEP) is effective in preventing HIV infection, but no study has evaluated combination prevention interventions with PrEP for transgender women (TGW) and men who have sex with men (MSM) who sell sex. Methods: The Combination Prevention Effectiveness (COPE) study was a community-based, non-randomized implementation study in Bangkok and Pattaya, Thailand. Participants were HIV-negative MSM and TGW aged 18–26 years who reported exchanging sex with men in the prior 12 months and who met 2014 U.S. Public Health Service PrEP eligibility criteria. The intervention included quarterly HIV testing, semiannual testing for sexually transmitted infections, provision of condoms with lubricant, and the opportunity to initiate or end daily oral PrEP use at any time during study participation. Participants taking PrEP received monthly adherence counseling and short message service reminders. The primary outcome was HIV incidence rate ratio (IRR) on PrEP vs. not on PrEP. Secondary outcomes were PrEP initiation, PrEP use at 12 months, and PrEP adherence. Findings: From October 2017 to August 2019, 846 participants were enrolled: 531 (62.8%) immediately initiated PrEP; 104 (12.3%) subsequently initiated PrEP, and 211 (24.9%) never initiated PrEP. Among those initiating PrEP within 30 days of enrollment; 85.9% were on PrEP at the 12-months. When taking PrEP, participants reported adherent PrEP use at 94.2% of quarterly assessments. Ten HIV seroconversions occurred without PrEP use (incidence rate [IR] = 3.42 per 100 person-years [PY]; 95% CI = 1.64–6.30), while zero cases occurred with PrEP use (IR = 0.0 per 100PY; 95% CI = 0.0–0.62), with IRR = 0.0 (95% CI = 0.0–0.22; p
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- 2023
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38. Signal-Induced Disassembly of the SCF Ubiquitin Ligase Complex by Cdc48/p97
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James L. Yen, Karin Flick, Peter K. Kaiser, Ikram Ouni, Christie V. Papagiannis, Lan Huang, An Tyrrell, Radhika Mathur, and Robyn M. Kaake
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Saccharomyces cerevisiae Proteins ,Cell Cycle Proteins ,Saccharomyces cerevisiae ,F-box protein ,Article ,Substrate Specificity ,SCF complex ,Adenosine Triphosphate ,SKP Cullin F-Box Protein Ligases ,Valosin Containing Protein ,Skp1 ,Cell division control protein 4 ,Molecular Biology ,chemistry.chemical_classification ,Adenosine Triphosphatases ,DNA ligase ,biology ,F-Box Proteins ,Hydrolysis ,Ubiquitination ,Cell Biology ,Ubiquitin ligase ,Cell biology ,chemistry ,Multiprotein Complexes ,biology.protein ,SCF ubiquitin ligase complex ,Cadmium ,Protein Binding ,Signal Transduction - Abstract
Summary A large group of E3 ubiquitin ligases is formed by the multisubunit SCF complex, whose core complex (Rbx1/Cul1-Cdc53/Skp1) binds one of many substrate recruiting F-box proteins to form an array of SCF ligases with diverse substrate specificities. It has long been thought that ubiquitylation by SCF ligases is regulated at the level of substrate binding. Here we describe an alternative mechanism of SCF regulation by active dissociation of the F-box subunit. We show that cadmium stress induces selective recruitment of the AAA + ATPase Cdc48/p97 to catalyze dissociation of the F-box subunit from the yeast SCF Met30 ligase to block substrate ubiquitylation and trigger downstream events. Our results not only provide an additional layer of ubiquitin ligase regulation but also suggest that targeted, signal-dependent dissociation of multisubunit enzyme complexes is an important mechanism in control of enzyme function.
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- 2012
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39. A Dominant Suppressor Mutation of the met30 Cell Cycle Defect Suggests Regulation of the Saccharomyces cerevisiae Met4-Cbf1 Transcription Complex by Met32
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Ning-Yuan Su, Christie V. Papagiannis, Ikram Ouni, and Peter K. Kaiser
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Saccharomyces cerevisiae Proteins ,Transcription, Genetic ,Saccharomyces cerevisiae ,Repressor ,Models, Biological ,Biochemistry ,F-box protein ,Gene Expression Regulation, Fungal ,Transcription, Chromatin, and Epigenetics ,Molecular Biology ,Transcription factor ,Genes, Dominant ,Models, Genetic ,biology ,Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ,F-Box Proteins ,Cell Cycle ,Ubiquitin-Protein Ligase Complexes ,Promoter ,Cell Biology ,Cell cycle ,biology.organism_classification ,Molecular biology ,Protein Structure, Tertiary ,Ubiquitin ligase ,Cell biology ,DNA-Binding Proteins ,Repressor Proteins ,Basic-Leucine Zipper Transcription Factors ,Gene Expression Regulation ,Mutation ,Transcription preinitiation complex ,biology.protein ,Transcription Factors - Abstract
Met30 is the substrate recognition subunit of the essential ubiquitin ligase SCF(Met30). The essential function of Met30 is the inactivation of the Saccharomyces cerevisiae transcription factor Met4, because fully activated Met4 induces a cell cycle arrest. Met4 regulates expression of genes involved in the sulfur assimilation pathway and coordinates the transcriptional program and cell cycle progression in response to cadmium and arsenic stress. Met4 lacks DNA binding activity and requires either Cbf1 or one of the two homologous proteins Met31 and Met32 for promoter association. Accordingly, met4 mutants, cbf1 mutants, and met31 met32 double mutants are methionine auxotroph. We isolated a truncated version of Met32 (Met32(Delta145-192)) as a dominant suppressor of the cell cycle defect of met30 mutants. Expression of Met32(Delta145-192) significantly reduced induction of Met4-regulated genes. Interestingly, both Cbf1- and Met31/32-dependent genes were affected by Met32(Delta145-192). Mechanistically, Met32(Delta145-192) prevented recruitment of Met4 to both Cbf1 and Met31/32-dependent promoters. We further demonstrated that Met32 is part of the Cbf1-Met4 complex bound to Cbf1-recruiting promoter elements and that Met31/32 are required for formation of a stable Met4-Cbf1 transcription complex. These results suggest a regulatory role of Met32 as part of the Cbf1-Met4 complex and provide molecular insight into coordination of cell cycle response and modulation of gene expression programs.
- Published
- 2008
40. Fis Targets Assembly of the Xis Nucleoprotein Filament to Promote Excisive Recombination by Phage Lambda
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Daniel Yoo, Robert T. Clubb, Mohamad A. Abbani, Christie V. Papagiannis, Reid C. Johnson, Duilio Cascio, and My D. Sam
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Protein Conformation ,Molecular Sequence Data ,Plasma protein binding ,Protomer ,Crystallography, X-Ray ,medicine.disease_cause ,DNA-binding protein ,Article ,Viral Proteins ,Protein structure ,Bacterial Proteins ,Structural Biology ,Factor For Inversion Stimulation Protein ,medicine ,Binding site ,Promoter Regions, Genetic ,Molecular Biology ,Recombination, Genetic ,Genetics ,Mutation ,Binding Sites ,Base Sequence ,biology ,Escherichia coli Proteins ,Membrane Proteins ,Lambda phage ,biology.organism_classification ,Bacteriophage lambda ,Nucleoproteins ,DNA Nucleotidyltransferases ,Biophysics ,Virus Activation ,Dimerization ,Protein Binding ,Transcription Factors - Abstract
The phage-encoded Xis protein is the major determinant controlling the direction of recombination in phage lambda. Xis is a winged-helix DNA binding protein that cooperatively binds to the attR recombination site to generate a curved microfilament, which promotes assembly of the excisive intasome but inhibits formation of an integrative intasome. We find that lambda synthesizes surprisingly high levels of Xis immediately upon prophage induction when excision rates are maximal. However, because of its low sequence-specific binding activity, exemplified by a 1.9 Å co-crystal structure of a nonspecifically bound DNA complex, Xis is relatively ineffective at promoting excision in vivo in the absence of the host Fis protein. Fis binds to a segment in attR that almost entirely overlaps one of the Xis binding sites. Instead of sterically excluding Xis binding from this site, as has been previously believed, we show that Fis enhances binding of all three Xis protomers to generate the microfilament. A specific Fis-Xis interface is supported by the effects of mutations within each protein, and relaxed, but not completely sequence-neutral, binding by the central Xis protomer is supported by the effects of DNA mutations. We present a structural model for the 50 bp curved Fis-Xis cooperative complex that is assembled between the arm and Holliday junction Int binding sites whose trajectory places constraints on models for the excisive intasome structure.
- Published
- 2007
41. Structure of the cooperative Xis–DNA complex reveals a micronucleoprotein filament that regulates phage lambda intasome assembly
- Author
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Duilio Cascio, Robert T. Clubb, Reid C. Johnson, Mohamad A. Abbani, Christie V. Papagiannis, and My D. Sam
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Virus Integration ,Protomer ,Regulatory Sequences, Nucleic Acid ,Crystallography, X-Ray ,law.invention ,Viral Proteins ,chemistry.chemical_compound ,law ,Escherichia coli ,Directionality ,Recombination, Genetic ,Genetics ,Binding Sites ,Multidisciplinary ,Base Sequence ,Integrases ,biology ,Nucleic acid sequence ,Cooperative binding ,DNA ,Biological Sciences ,Chromosomes, Bacterial ,Lambda phage ,biology.organism_classification ,Bacteriophage lambda ,Integrase ,Nucleoproteins ,chemistry ,Multiprotein Complexes ,DNA Nucleotidyltransferases ,Biophysics ,biology.protein ,Recombinant DNA ,Nucleic Acid Conformation ,Protein Binding - Abstract
The DNA architectural protein Xis regulates the construction of higher-order nucleoprotein intasomes that integrate and excise the genome of phage lambda from the Escherichia coli chromosome. Xis modulates the directionality of site-specific recombination by stimulating phage excision 10 6 -fold, while simultaneously inhibiting phage reintegration. Control is exerted by cooperatively assembling onto a ≈35-bp DNA regulatory element, which it distorts to preferentially stabilize an excisive intasome. Here, we report the 2.6-Å crystal structure of the complex between three cooperatively bound Xis proteins and a 33-bp DNA containing the regulatory element. Xis binds DNA in a head-to-tail orientation to generate a micronucleoprotein filament. Although each protomer is anchored to the duplex by a similar set of nonbase specific contacts, malleable protein–DNA interactions enable binding to sites that differ in nucleotide sequence. Proteins at the ends of the duplex sequence specifically recognize similar binding sites and participate in cooperative binding via protein–protein interactions with a bridging Xis protomer that is bound in a less specific manner. Formation of this polymer introduces ≈72° of curvature into the DNA with slight positive writhe, which functions to connect disparate segments of DNA bridged by integrase within the excisive intasome.
- Published
- 2007
42. Choreographies
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Derrida, Jacques, McDonald, Christie V., Masó, Joana, and Bassas Vila (trad.), Javier
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Jacques Derrida ,filosofía ,diferancia ,género ,feminismos - Abstract
Traducción al castellano del intercambio de correspondencia entre Jacques Derrida y Christie V. McDonald, en el otoño de 1981. A partir de las preguntas de McDonald, el filósofo reflexiona en torno a temas como el género, la diferencia sexual, los movimientos feministas, los feminismos, el lugar de la mujer, y el aporte que una perspectiva como la deconstrucción ofrece a su abordaje., Translation to Spanish of the correspondence between Jacques Derrida and Christie V. McDonald during the autumn of 1981. Considering McDonald’s inquiries, the philosopher reflects on subjects such as gender, sexual difference, feminist movements, feminisms, the woman’s place, and the contribution that a perspective such as deconstruction offers to these matters.
- Published
- 2010
43. Optimising student-led interprofessional learning across eleven health disciplines
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Christie van Diggele, Chris Roberts, and Inam Haq
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Interprofessional learning ,Interdisciplinary ,Health ,Large-scale ,Assessment ,Education ,Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract Background Provision of effective Interprofessional learning (IPL) opportunities plays a vital role in preparing healthcare students for future collaborative practice. There is an identified need for universities to better prepare students for interprofessional teamwork, however, few large-scale IPL activities have been reported. Additionally, little has been reported on disciplinary differences in student learning experience. The Health Collaboration Challenge (HCC) is a large-scale IPL activity held annually at the University of Sydney. This study sought to explore students’ experience of early participation in an interprofessional case-based learning activity, and the similarities and differences in the perceived value of interprofessional (social) learning for each discipline. Methods In 2018, 1674 students from 11 disciplines (dentistry, oral health, nursing, pharmacy, medicine, occupational therapy, speech pathology, physiotherapy, dietetics, diagnostic radiography, exercise physiology) participated in the HCC. Students worked in teams to produce a video and patient management plan based on a patient case. Participants completed a questionnaire, including closed and open-ended items. Quantitative data were analysed using descriptive statistics. Thematic analysis was used to code and categorise qualitative data into themes. These themes were then applied and quantified at a disciplinary level to measure prevalence. Results In total, 584/1674 (35%) of participants responded to the questionnaire. Overall, students perceived their experience to be largely beneficial to their learning and interprofessional skill development. Positive aspects included opportunities for peer learning, collaboration, networking, and understanding the different roles and responsibilities of other health professions. Negative aspects included the video form of assessment, inequity in assessment weighting across disciplines, the discipline mix within teams and lack of case relevance. Conclusion The learning activity provided a framework for students to practice and develop their skills in interprofessional teamwork, as they prepare for increased clinical placements. Overall, students perceived their experience as beneficial to their learning and professional development early in their degree. However, they expressed dissatisfaction with the inequity of assessment weighting across the disciplines; lack of relevance of the case across disciplines; and the activity of producing a video. Further research is needed regarding the ideal number of disciplines to include in teamwork specific to a patient case.
- Published
- 2021
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44. Coreografías
- Author
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Derrida, Jacques and McDonald, Christie V.
- Subjects
Jacques Derrida ,Feminismes ,Diferancia ,Filosofía ,Género ,Filosofia ,Gènere ,Feminismos - Abstract
Traducción al castellano del intercambio de correspondencia entre Jacques Derrida y Christie V. McDonald, en el otoño de 1981. A partir de las preguntas de McDonald, el filósofo reflexiona en torno a temas como el género, la diferencia sexual, los movimientos feministas, los feminismos, el lugar de la mujer, y el aporte que una perspectiva como la deconstrucción ofrece a su abordaje.
- Published
- 2008
45. Interprofessional education: tips for design and implementation
- Author
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Christie van Diggele, Chris Roberts, Annette Burgess, and Craig Mellis
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Interprofessional education ,Multidisciplinary ,Facilitation ,Teamwork ,Collaboration ,Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract Interprofessional education (IPE) is a critical approach for preparing students to enter the health workforce, where teamwork and collaboration are important competencies. IPE has been promoted by a number of international health organisations, as part of a redesign of healthcare systems to promote interprofessional teamwork, to enhance the quality of patient care, and improve health outcomes. In response, universities are beginning to create and sustain authentic and inclusive IPE activities, with which students can engage. A growing number of health professionals are expected to support and facilitate interprofessional student groups. Designing interprofessional learning activities, and facilitating interprofessional groups of students requires an additional layer of skills compared with uniprofessional student groups. This article outlines the key points for planning and practicing interprofessional facilitation within the classroom and clinical setting.
- Published
- 2020
- Full Text
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46. Introduction to the Peer Teacher Training in health professional education supplement series
- Author
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Annette Burgess, Christie van Diggele, Chris Roberts, and Craig Mellis
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Peer teacher training ,Clinical teacher training ,Interprofessional ,Feedback ,Professional skills ,Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract Skills in supervision, teaching, facilitation, assessment and feedback, leadership and interprofessional teamwork are required graduate attributes for health professionals. Despite this, the opportunity for learning these skills is rarely embedded within undergraduate and postgraduate health professional training curricula. Additionally, there are limited examples of interprofessional delivery of teaching programs. Since teaching skills can be learned, healthcare faculties play an important role in improving the teaching abilities of their students. At the University of Sydney, we developed and implemented interprofessional, blended learning teacher training programs for health professional students, and junior health professionals: The Peer Teacher Training (PTT) program, and the Clinical Teacher Training (CTT) program. Based on our successful programs, this paper provides an introduction to our Peer Teacher Training supplement. Namely, 11 articles designed to assist those who work and teach in a clinical context; address key challenges; and provide practical tips and frameworks to assist in teaching, assessment, and feedback.
- Published
- 2020
- Full Text
- View/download PDF
47. Facilitating small group learning in the health professions
- Author
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Annette Burgess, Christie van Diggele, Chris Roberts, and Craig Mellis
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Small group learning ,Team building skills ,Facilitation ,Peer assisted learning ,Health professional students ,Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract There is now good evidence that small group teaching provides a fruitful academic environment, which optimises learning, particularly in the healthcare setting, and especially when compared to lectures. An individual student’s understanding of knowledge is increased when they are able to actively compare and build on their own understanding in conjunction with their peers. Small group teaching provides opportunities for learners to work collaboratively, and promotes team-building skills – skills that are essential to work within healthcare settings. The aim of this paper is to provide health professional students and early career health professionals involved in peer and near peer teaching, with an overview of approaches and tips to improve learner engagement when facilitating small groups.
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- 2020
- Full Text
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48. Planning peer assisted learning (PAL) activities in clinical schools
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Annette Burgess, Christie van Diggele, Chris Roberts, and Craig Mellis
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Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract Peer Assisted Learning (PAL) is well accepted as an educational method within health professional education, involving a process of socialisation among students. PAL activities provide a framework whereby students are permitted to practice and develop their healthcare and teaching skills. However, the success of PAL activities is dependent upon two key factors: the “agency” of the individual students, that is, their willingness to participate; and importantly, the “affordance” of the activity, that is, the invitational quality provided by the clinical school. The purpose of this paper is to assist healthcare educators and administrators responsible for curriculum design, course co-ordination, and educational research, in developing their own PAL activities. Health professional students and junior health professionals leading or participating in PAL activities may also find the paper useful. Based on the authors’ collective experience, and relevant literature, we provide practical tips for the design, implementation and evaluation of PAL activities.
- Published
- 2020
- Full Text
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49. Tips for teaching procedural skills
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Annette Burgess, Christie van Diggele, Chris Roberts, and Craig Mellis
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Procedural skills teaching ,Peyton’s four-step approach ,Determining competency ,Provision of feedback ,Deliberate practice ,Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract The teaching of procedural skills required for clinical practice remains an ongoing challenge in healthcare education. Health professionals must be competent to perform a wide range of clinical skills, and are also regularly required to teach these clinical skills to their peers, junior staff, and students. Teaching of procedural skills through the use of frameworks, observation and provision of feedback, with opportunities for repeated practice assists in the learners’ acquisition and retention of skills. With a focus on the teaching of non-complex skills, this paper explores how skills are learned; ways to improve skill performance; determining competency; and the provision of effective feedback.
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- 2020
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50. Feedback in the clinical setting
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Annette Burgess, Christie van Diggele, Chris Roberts, and Craig Mellis
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Feedback ,Peer teaching ,Clinical teaching ,Student peer-to-peer feedback ,Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract Provision of feedback forms an integral part of the learning process. Receipt of feedback enriches the learning experience, and helps to narrow the gap between actual and desired performance. Effective feedback helps to reinforce good practice, motivating the learner towards the desired outcome. However, a common complaint from learners is that the receipt of feedback is infrequent and inadequate. This paper briefly explores the role of feedback within the learning process, the barriers to the feedback process, and practical guidelines for facilitating feedback.
- Published
- 2020
- Full Text
- View/download PDF
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