28 results on '"Catalani S"'
Search Results
2. Linee guida per il monitoraggio biologico
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Mutti, A, De Palma, G, Andreoli, R, Bonini, S, Bollati, V, Carrieri, M, Catalani, S, Coggiola, M, Fustinoni, S, Gobba, Fabriziomaria, Goldoni, M, Manno, M, Perbellini, L, Scapellato, Ml, and Apostoli, P.
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monitoraggio biologico - Published
- 2013
3. SELECTED ORAL COMMUNICATION SESSION, SESSION 70: ANDROLOGY AND SEMINAL FACTORS Wednesday 6 July 2011 14:00 - 15:45
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Caballero Peregrin, P., primary, Nunez-Calonge, R., additional, Guijarro, J. A., additional, Ortega, L., additional, Cortes, S., additional, Gosalvez, J., additional, Palini, S., additional, De Stefani, S., additional, Scala, V., additional, Benedetti, S., additional, Tagliamonte, M. C., additional, Catalani, S., additional, Primiterra, M. A., additional, Polli, V., additional, Rocchi, P., additional, Tiezzi, A., additional, Donati, L., additional, Pelosi, E., additional, Canestrari, F., additional, Bulletti, C., additional, Garcia-Herrero, S., additional, Meseguer, M., additional, Martinez-Conejero, J. A., additional, Romany, L., additional, Ruiz, M., additional, Horcajadas, J. A., additional, Pellicer, A., additional, Garrido, N., additional, Ramon, O., additional, Corcostegui, B., additional, Crisol, L., additional, Exposito, A., additional, Mugica, J., additional, Matorras, R., additional, Kyurkchiev, S., additional, Dyulgerova-Nikolova, D., additional, Milachich, T., additional, Shterev, A., additional, Pons Mallol, I., additional, Cercas Duque, R., additional, Villas Martin, C., additional, Brana Pelayo, C., additional, Fernandez Shaw, S., additional, Arts, E. G. J. M., additional, Wester, N. E., additional, Groen, H., additional, van Echten-Arends, J., additional, and Land, J. A., additional
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- 2011
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4. Development of a New Sequential Extraction Procedure of Nickel Species on Workplace Airborne Particulate Matter: Assessing the Occupational Exposure to Carcinogenic Metal Species
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Catalani Simona, Fostinelli Jacopo, Gilberti Maria Enrica, Orlandi Francesca, Magarini Riccardo, Paganelli Matteo, Madeo Egidio, and De Palma Giuseppe
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Analytical chemistry ,QD71-142 - Abstract
Nickel (Ni) compounds and metallic Ni have many industrial and commercial applications, including their use in the manufacturing of stainless steel. Due to the specific toxicological properties of the different Ni species, there is a growing interest about the availability of analytical methods that allow specific risk assessment, particularly related to exposure to the Ni species classified as carcinogenic. In this paper, we described a speciation method of inorganic Ni compounds in airborne particulate matter, based on selective sequential extractions. The analytical method described in this paper allows the determination of soluble, sulfidic, metallic, and oxide Ni by a simple sequential extraction procedure and determination by Atomic Absorption Spectroscopy using small volumes of solutions and without long evaporation phases. The method has been initially set up on standard laboratory mixtures of known concentrations of different Ni salts. Then it has then been tested on airborne particulate matter (powder and filters) collected in different workstations of a large stainless steel production facility. The method has occurred effectively in the comparison of the obtained results with occupational exposure limit values set by the main international scientific and regulatory agencies for occupational safety and health, in order to prevent both toxic and carcinogenic effects in humans.
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- 2018
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5. The role of albumin in human toxicology of cobalt: contribution from a clinical case.
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Catalani S, Leone R, Rizzetti MC, Padovani A, and Apostoli P
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The distribution and adverse effects, especially to optic and acoustic nerves, of cobalt released from a hip arthroplasty and its association with albumin were studied. The analysis of cobalt was performed in plasma, whole blood, urine, and cerebrospinal fluid by inductively coupled plasma mass spectrometry (ICP-MS). The fraction of albumin binding the metal was determined by colorimetric assay using dithiothreitol (DTT). In all the biological matrices very high levels of cobalt were measured, but contrary to expected, a higher concentration in whole blood than in plasma was observed. The determination of altered albumin confirmed this hypothesis. This evidence might indicate an alteration in the binding of cobalt to albumin and a consequent increase in the concentration of the diffusible (free) fraction of the metal. This appears an interesting starting point for further investigations for identifying and better understanding cobalt neurotoxicity, apparently not so frequent in occupational medicine and clinical practice. [ABSTRACT FROM AUTHOR]
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- 2011
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6. Cushion bearings versus large diameter head metal-on-metal bearings in total hip arthroplasty: a short-term metal ion study
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Martha Hoque, Antonio Moroni, Elena Diremigio, Simona Catalani, Dario Buffoli, Francesco Cantù, Pietro Apostoli, Emanuele Nocco, Moroni A, Nocco E, Hoque M, Diremigio E, Buffoli D, Cantù F, Catalani S, Apostoli P, Moroni, Antonio, Nocco, E, Hoque, M, Diremigio, E, Buffoli, D, Cantù, F, Catalani, S, and Apostoli, P.
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Adult ,Chromium ,Male ,Reoperation ,medicine.medical_specialty ,Arthroplasty, Replacement, Hip ,BEARING COUPLES ,Prosthesis Design ,Osteoarthritis, Hip ,law.invention ,Clinical study ,Femoral head ,law ,medicine ,Humans ,Orthopedics and Sports Medicine ,Prospective Studies ,Large diameter ,Hip Dislocation, Congenital ,Aged ,Polycarboxylate Cement ,Bearing (mechanical) ,business.industry ,Cobalt ,General Medicine ,Middle Aged ,Surgery ,Treatment Outcome ,medicine.anatomical_structure ,Polycarbonate urethane ,Orthopedic surgery ,Cushion ,Female ,Hip Prosthesis ,business ,Follow-Up Studies ,Total hip arthroplasty - Abstract
INTRODUCTION: Metal-on-metal total hip arthroplasty (MOM THA) has the advantage of replicating the femoral head size, but the postoperative elevation of serum metal ion levels is a cause for concern. Metal-on-polycarbonate-urethane is a new cushion bearing featuring a large diameter metal head coupled with a polycarbonate-urethane liner. AIM: The aim of this study was to assess and compare serum cobalt (Co) and chromium (Cr) levels in a group of 15 patients treated with a cushion bearing THA system (Group A) and a group of 15 patients treated with a MOM THA system (Group B) at short-term. At a mean follow-up of 27.3 months (18-35 months), in Group A the median Cr and Co serum levels were significantly lower than in Group B, measuring 0.24 μg/L (0.1-2.1 μg/L) and 0.6 μg/L (0.29-2.3 μg/L) compared to 1.3 μg/L (0.1-9 μg/L, p < 0.001) and 2.9 μg/L (0.85-13.8 μg/L, p < 0.001) respectively. RESULTS: All patients demonstrated an excellent clinical result, as shown by the Harris and Oxford hip scores. The cushion bearing THA studied in this paper showed clinical outcomes similar to the MOM THA bearing, with the advantage of no significant metal ion elevation in the serum. CONCLUSION: These findings warrant the continued clinical study of compliant bearing options.
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- 2012
7. Occupational exposure to formaldehyde and risk of non hodgkin lymphoma: A meta-Analysis
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Egidio Madeo, Francesca De' Donato, Giuseppe De Palma, Kenneth A. Mundt, Enrico Pira, Simona Catalani, Pietro Apostoli, Paolo Boffetta, Catalani S., Donato F., Madeo E., Apostoli P., De Palma G., Pira E., Mundt K.A., and Boffetta P.
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Oncology ,Meta-Analysi ,Cancer Research ,medicine.medical_specialty ,Formaldehyde ,010501 environmental sciences ,Risk Assessment ,lcsh:RC254-282 ,01 natural sciences ,Cancer ,Meta-Analysis ,Non-Hodgkin lymphoma ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Risk Factors ,Occupational Exposure ,Internal medicine ,Genetics ,medicine ,Humans ,0105 earth and related environmental sciences ,business.industry ,Lymphoma, Non-Hodgkin ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Random effects model ,medicine.disease ,030210 environmental & occupational health ,Confidence interval ,Lymphoma ,Occupational Diseases ,chemistry ,Relative risk ,Meta-analysis ,Population study ,Occupational exposure ,business ,Disinfectants ,Research Article - Abstract
Background Formaldehyde, a widely used chemical, is considered a human carcinogen. We report the results of a meta-analyses of studies on the relationship between occupational exposure to formaldehyde and risk of non-Hodgkin lymphoma (NHL). Methods We performed a systematic review and meta-analysis according to international guidelines and we identified 12 reports of occupational populations exposed to formaldehyde. We evaluated inter-study heterogeneity and we applied a random effects model. We conducted a cumulative meta-analysis and a meta-analysis according to estimated average exposure of each study population. Results The meta-analysis resulted in a summary relative risk (RR) for NHL of 0.93 (95% confidence interval 0.83–1.04). The cumulative meta-analysis suggests that higher RRs were detected in studies published before 1986, while studies available after 1986 did not show an association. No differences were found between different levels of occupational exposure. Conclusions Notwithstanding some limitations, the results of this meta-analysis do not support the hypothesis of an association between occupational exposure to formaldehyde and risk of NHL.
- Published
- 2019
8. In vivo response of heme-oxygenase-1 to metal ions released from metal-on-metal hip prostheses
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Alina Beraudi, Aldo Toni, Simona Catalani, Federica Facchin, Silvia Canaider, Susanna Stea, Eva Bianconi, Pietro Apostoli, Dalila De Pasquale, Barbara Bordini, Beraudi, A, Bianconi, E, Catalani, S, Canaider, S, De Pasquale, D, Apostoli, P, Bordini, B, Stea, S, Toni, A, and Facchin, F
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0301 basic medicine ,Chromium ,Male ,Cancer Research ,Antioxidant ,medicine.medical_treatment ,Metal ions in aqueous solution ,Gene Expression ,Reductase ,Biochemistry ,Heme-oxygenase-1 ,Superoxide dismutase ,Metal-on-metal hip prosthesi ,03 medical and health sciences ,0302 clinical medicine ,Gene expression ,Genetics ,medicine ,Humans ,Molecular Biology ,Aged ,chemistry.chemical_classification ,Ions ,030222 orthopedics ,biology ,Glutathione peroxidase ,Cobalt ,Middle Aged ,Heme oxygenase ,030104 developmental biology ,Enzyme ,Oncology ,chemistry ,Oxidative stress ,Metals ,biology.protein ,Metal-on-Metal Joint Prostheses ,Molecular Medicine ,Female ,Hip Prosthesis ,Heme Oxygenase-1 - Abstract
Metal ion release and accumulation is considered to be a factor responsible for the high failure rates of metal-on-metal (MoM) hip implants. Numerous studies have associated the presence of these ions, besides other factors, including a hypoxia‑like response and changes in pH due to metal corrosion leading to the induction of the oxidative stress response. The aim of the present study was to verify whether, in patients with a MoM hip prosthesis, mRNA and protein expression of HMOX‑1 was modulated by the presence of metal ions and whether patients without prostheses exhibit a different expression pattern of this enzyme. The study was conducted on 22 matched pairs of patients with and without prostheses, for a total of 44 samples. Ion dosage was determined using inductively coupled plasma mass spectrometry equipped with dynamic cell reaction. HMOX‑1 gene expression was quantified by reverse transcription-quantitative polymerase chain reaction and HMOX‑1 protein expression was analyzed using an enzyme-linked immunosorbent assay. The results demonstrated that although there were significant differences in the metallic ion concentrations amongst the two groups of patients, there was no correlation between circulating levels of cobalt (Co) and chromium (Cr), and HMOX‑1 gene and protein expression. Additionally, there was no significant difference in the protein expression levels of HMOX‑1 between the two groups. In conclusion, it was demonstrated that circulating Co and Cr ions released by articular prosthetics do not induce an increase in HMOX‑1 mRNA and protein expression at least 3.5 years after the implant insertion. The present study suggests that involvement of HMOX‑1 may be excluded from future studies and suggests that other antioxidant enzymes, including superoxide dismutase, glutathione peroxidase and reductase should be investigated.
- Published
- 2015
9. Correction: CELLFOOD™ induces apoptosis in human mesothelioma and colorectal cancer cells by modulating p53, c-myc and pAkt signaling pathways.
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Nuvoli B, Santoro R, Catalani S, Battistelli S, Benedetti S, Canestrari F, and Galati R
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- 2022
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10. A microplate-based DCFH-DA assay for the evaluation of oxidative stress in whole semen.
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Benedetti S, Catalani S, De Stefani S, Primiterra M, Fraternale A, Palma F, and Palini S
- Abstract
Aims: The well-documented relationship between sperm oxidation and male infertility strongly encourages the development of assays for reactive oxygen species detection in semen samples. The present study aims to apply the microplate-based 2',7'-dichlorofluorescein diacetate assay to the evaluation of oxidative stress in unprocessed whole semen, thus avoiding sample centrifugations and other manipulations that may cause significant reactive oxygen species increments., Main Methods: The fluorescence assay consisted in the quantification of both intracellular and extracellular reactive oxygen species levels in unwashed semen specimens by using the probe 2',7'-dichlorofluorescein diacetate into a 96-well plate. The method was useful for the preliminary assessment of the oxidation levels of whole semen samples from men undergoing standard sperm analysis as well as to evaluate the effect of some pro-glutathione molecules on semen oxidative status., Key Findings: The 2',7'-dichlorofluorescein diacetate assay was successfully adapted to the evaluation of oxidative stress in whole semen, effectively revealing the perturbation of the redox homeostasis of the sample. Accordingly, specimens with abnormal sperm parameters (n = 10) presented oxidation indexes significantly higher than those with normospermia (n = 10) [7729 (range 3407-12769) vs. 1356 (range 470-2711), p < 0.001]; in addition, semen oxidation indexes negatively correlated to sperm motility and morphology. Noteworthy, whole semen exposure to pro-glutathione compounds led to reduced semen oxidation levels and sperm protection against oxidative damage., Significance: Based on our pilot experimental data, the microplate-based 2',7'-dichlorofluorescein diacetate assay appears to be a convenient method for the detection of reactive oxygen species levels in whole semen samples, avoiding artifacts due to semen centrifugation steps. At the same time, the test could be a helpful tool for the basic and quick screening of antioxidant molecules able to preserve semen quality., Competing Interests: The authors declare no conflict of interest., (© 2022 The Author(s).)
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- 2022
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11. Systematic review and meta-analysis of epidemiological studies on the association of occupational exposure to free crystalline silica and systemic lupus erythematosus.
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Morotti A, Sollaku I, Catalani S, Franceschini F, Cavazzana I, Fredi M, Sala E, and De Palma G
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- Epidemiologic Studies, Humans, Lupus Erythematosus, Systemic chemically induced, Occupational Exposure adverse effects, Silicon Dioxide adverse effects
- Abstract
Objectives: Some evidence suggests that exposure to free crystalline silica may contribute to the risk of developing SLE. A systematic search was carried out for all published epidemiological studies concerning this association. A meta-analysis was conducted on relevant studies., Methods: We searched PubMed and EMBASE databases for original articles published from 1960 to November 2019 in any language. In addition, we also searched the reference lists of included studies manually for additional relevant articles. Finally, seven studies were included in the systematic review and six studies in the meta-analysis (four case-control and two cohort studies). The odds ratio and 95% CI were calculated using a random effect meta-analysis., Results: The meta-analysis of the studies, applying a random effect model, yielded an overall odds ratio of 3.49 (95% CI, 1.24, 9.83), with I2 = 92.36% (pronounced heterogeneity). We also stratified the meta-analysis by study design; case-control studies: odds ratio 1.85 (95% CI, 0.96, 3.59) with I2 = 75.92%; and cohort studies (cases with silicosis): odds ratio 9.71 (95% CI, 1.13, 83.58) with I2 = 72.65%., Conclusions: The obtained results support the hypothesis of a possible association between occupational exposure to free crystalline silica and SLE, in particular at higher exposure levels, known to induce silicosis. The studies that have investigated this association are still scarce and the heterogeneity between the studies remains high. New studies are deemed necessary to confirm the association., (© The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
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- 2021
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12. Health effects of living near an incinerator: A systematic review of epidemiological studies, with focus on last generation plants.
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Negri E, Bravi F, Catalani S, Guercio V, Metruccio F, Moretto A, La Vecchia C, and Apostoli P
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- Environmental Exposure, Epidemiologic Studies, Female, Humans, Pregnancy, Air Pollutants toxicity, Incineration, Neoplasms chemically induced, Neoplasms epidemiology, Respiration Disorders epidemiology
- Abstract
Huge reductions in incinerators' emissions occurred over time, and results of older studies cannot be directly generalized to modern plants. We conducted a systematic review of the epidemiologic evidence of the health effects of incinerators, classifying plants in three generations, according to emission limits. A systematic search identified 63 epidemiologic studies, published in English, investigating health effects of incinerators on humans. We focused on cancer, cardio-cerebrovascular diseases (CVD) and respiratory diseases, pregnancy outcomes and congenital anomalies. Only six studies in the general population were on third generation incinerators providing data on pregnancy outcomes and congenital anomalies. Given the heterogeneity of methods, the abundance of ecological/semi-ecological studies and the lack of reliable quantitative measures of exposure in several studies we did not perform any meta-analysis. No excesses emerged concerning all cancers and lung cancer. An excess of non-Hodgkin lymphoma was reported in some earlier studies, but not for second generation plants. Possible excesses of soft tissue sarcomas were confined to earlier incinerators and the areas closer to the plants. No clear association emerged for CVD and diseases of the respiratory system. Several different pregnancy outcomes were considered, and no consistent association emerged, in spite of a few positive results. Studies were negative for congenital anomalies as a whole. Sporadic excesses were reported in a few studies for specific types of anomalies, but no consistent pattern emerged. Evaluation of the evidence was hindered by heterogeneity in reporting and classification of outcomes across studies. Direct evidence from third generation plants is scarce. Methodological issues in study design (mainly related to exposure assessment, confounding and ecological design) and analysis make interpretation of results complex. In spite of this, the overall evidence suggests that, if there were any excesses at all for older incinerators, they were modest at most. Additional monitoring of third generation plants needs to overcome methodological weaknesses., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020. Published by Elsevier Inc.)
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- 2020
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13. Occupational exposure to formaldehyde and risk of non hodgkin lymphoma: a meta-analysis.
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Catalani S, Donato F, Madeo E, Apostoli P, De Palma G, Pira E, Mundt KA, and Boffetta P
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- Humans, Lymphoma, Non-Hodgkin chemically induced, Occupational Diseases chemically induced, Occupational Exposure adverse effects, Risk Assessment methods, Risk Factors, Disinfectants poisoning, Formaldehyde poisoning, Lymphoma, Non-Hodgkin epidemiology, Occupational Diseases epidemiology, Occupational Exposure statistics & numerical data
- Abstract
Background: Formaldehyde, a widely used chemical, is considered a human carcinogen. We report the results of a meta-analyses of studies on the relationship between occupational exposure to formaldehyde and risk of non-Hodgkin lymphoma (NHL)., Methods: We performed a systematic review and meta-analysis according to international guidelines and we identified 12 reports of occupational populations exposed to formaldehyde. We evaluated inter-study heterogeneity and we applied a random effects model. We conducted a cumulative meta-analysis and a meta-analysis according to estimated average exposure of each study population., Results: The meta-analysis resulted in a summary relative risk (RR) for NHL of 0.93 (95% confidence interval 0.83-1.04). The cumulative meta-analysis suggests that higher RRs were detected in studies published before 1986, while studies available after 1986 did not show an association. No differences were found between different levels of occupational exposure. Conclusions Notwithstanding some limitations, the results of this meta-analysis do not support the hypothesis of an association between occupational exposure to formaldehyde and risk of NHL.
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- 2019
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14. Erratum to "Development of a New Sequential Extraction Procedure of Nickel Species on Workplace Airborne Particulate Matter: Assessing the Occupational Exposure to Carcinogenic Metal Species".
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Catalani S, Fostinelli J, Gilberti ME, Orlandi F, Magarini R, Paganelli M, Madeo E, and De Palma G
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[This corrects the article DOI: 10.1155/2018/3812795.].
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- 2019
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15. Composition of Metallic Elements and Size Distribution of Fine and Ultrafine Particles in a Steelmaking Factory.
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Marcias G, Fostinelli J, Catalani S, Uras M, Sanna AM, Avataneo G, De Palma G, Fabbri D, Paganelli M, Lecca LI, Buonanno G, and Campagna M
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- Aerosols analysis, Humans, Italy, Metallurgy, Metals analysis, Occupational Exposure standards, Air Pollutants, Occupational analysis, Air Pollutants, Occupational chemistry, Environmental Monitoring, Metals chemistry, Occupational Exposure analysis, Particle Size, Particulate Matter analysis, Particulate Matter chemistry
- Abstract
Background: The characteristics of aerosol, in particular particle size and chemical composition, can have an impact on human health. Particle size distribution and chemical composition is a necessary parameter in occupational exposure assessment conducted in order to understand possible health effects. The aim of this study was to characterize workplace airborne particulate matter in a metallurgical setting by synergistically using two different approaches; Methodology: Analysis of inhalable fraction concentrations through traditional sampling equipment and ultrafine particles (UFP) concentrations and size distribution was conducted by an Electric Low-Pressure Impactor (ELPI+™). The determination of metallic elements (ME) in particles was carried out by inductively coupled plasma mass spectrometry; Results: Inhalable fraction and ME concentrations were below the limits set by Italian legislation and the American Conference of Governmental Industrial Hygienists (ACGIH, 2017). The median of UFP was between 4.00 × 10⁴ and 2.92 × 10⁵ particles/cm³. ME concentrations determined in the particles collected by ELPI show differences in size range distribution; Conclusions: The adopted synergistic approach enabled a qualitative and quantitative assessment of the particles in steelmaking factories. The results could lead to a better knowledge of occupational exposure characterization, in turn affording a better understanding of occupational health issues due to metal fumes exposure.
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- 2018
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16. Impact of the 24-h ultramarathon race on homocysteine, oxidized low-density lipoprotein, and paraoxonase 1 levels in professional runners.
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Benedetti S, Catalani S, Peda F, Luchetti F, Citarella R, and Battistelli S
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- Adult, Female, Humans, Italy, Male, Middle Aged, Aryldialkylphosphatase blood, Homocysteine blood, Lipoproteins, LDL blood, Running
- Abstract
The impact of the 24-h ultramarathon race on homocysteine (Hcy) and oxidized low-density lipoprotein (oxLDL) levels, two well-recognized cardiovascular risk factors, has not been deeply investigated. Similarly, no information exists on paraoxonase 1 (PON1), an antioxidant enzyme associated with high-density lipoproteins, which may detoxify oxLDL and Hcy-thiolactone, hence preventing their proatherogenic action. Taking this into account, a competitive 24-h ultramarathon race was organized in Reggio-Emilia (Italy) recruiting professional runners (n = 14) from the Italian Ultramarathon and Trail Association. Blood samples were collected from each participant before, during (14 h), and immediately after (24 h) the competition, thus to monitor the serum changes in Hcy, oxLDL, and PON1 levels, as well as other oxidative stress-related parameters, namely reactive oxygen metabolites (ROM) and total antioxidant capacity (PAT). As a result, a significant PON1 increase was recorded after 14 h of racing that persisted until the end of the performance. The same trend was observed for PAT values, which positively correlated to PON1 levels (R = 0.643, P<0.001). Hcy, oxLDL, and ROM remained almost unchanged throughout the competition. In conclusion, the present study suggested a protective role of PON1 in sustaining the antioxidant defense system and contrasting lipoprotein oxidative modifications over the 24-h race, and did not specifically evidence either Hcy or oxLDL accumulation in such challenging sporting events.
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- 2018
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17. Oxidative stress and apoptosis induction in human thyroid carcinoma cells exposed to the essential oil from Pistacia lentiscus aerial parts.
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Catalani S, Palma F, Battistelli S, and Benedetti S
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- Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic isolation & purification, Caco-2 Cells, Hep G2 Cells, Humans, Oils, Volatile chemistry, Oils, Volatile isolation & purification, Thyroid Neoplasms metabolism, Thyroid Neoplasms pathology, Antineoplastic Agents, Phytogenic pharmacology, Apoptosis drug effects, Oils, Volatile pharmacology, Oxidative Stress drug effects, Pistacia chemistry, Plant Components, Aerial chemistry, Thyroid Neoplasms drug therapy
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Background: Essential oils from the aerial parts (leaves, twigs and berries) of Pistacia lentiscus (PLEO) have been well characterized for their antibacterial and anti-inflammatory properties; however, poor information exists on their potential anticancer activity., Methods: Increasing concentrations of PLEO (0.01-0.1% v/v, 80-800 μg/ml) were administered to a wide variety of cultured cancer cells from breast, cervix, colon, liver, lung, prostate, and thyroid carcinomas. Fibroblasts were also included as healthy control cells. Cell viability was monitored by WST-8 assay up to 72 hours after PLEO administration. The intracellular formation of reactive oxygen species (ROS), the induction of apoptosis, and the enhancement of chemotherapeutic drug cytotoxicity by PLEO were further investigated in the most responsive cancer cell line., Results: A dose-dependent reduction of tumor cell viability was observed upon PLEO exposure; while no cytotoxic effect was revealed in healthy fibroblasts. FTC-133 thyroid cancer cells were found to be the most sensitive cells to PLEO treatment; accordingly, an intracellular accumulation of ROS and an activation of both the extrinsic and intrinsic apoptotic pathways were evidenced in FTC-133 cells after PLEO administration. Furthermore, the cytotoxic effect of the antineoplastic drugs cisplatin, 5-fluorouracil and etoposide was enhanced in PLEO-exposed FTC-133 cells., Conclusion: Taking into account its mode of action, PLEO might be considered as a promising source of natural antitumor agents which might have therapeutic potential in integrated oncology., Competing Interests: The authors have declared that no competing interests exist.
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- 2017
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18. Inhibitory effects of Aphanizomenon flos-aquae constituents on human UDP-glucose dehydrogenase activity.
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Scoglio S, Lo Curcio V, Catalani S, Palma F, Battistelli S, and Benedetti S
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- Cell Line, Tumor, Cell Proliferation drug effects, Drug Screening Assays, Antitumor, Humans, Male, Aphanizomenon chemistry, Enzyme Inhibitors pharmacology, Uridine Diphosphate Glucose Dehydrogenase antagonists & inhibitors
- Abstract
Objective: The purpose of this study was to investigate the in vitro inhibitory effects of the edible microalga Aphanizomenon flos-aquae (AFA) on human UDP-α-d-glucose 6-dehydrogenase (UGDH) activity, a cytosolic enzyme involved both in tumor progression and in phytochemical bioavailability., Methods: Both the hydrophilic and ethanolic AFA extracts as well as the constitutive active principles phycocyanin (PC), phycocyanobilin (PCB) and mycosporine-like amino acids (MAAs) were tested., Results: Among AFA components, PCB presented the strongest inhibitory effect on UGDH activity, acting as a competitive inhibitor with respect to UDP-glucose and a non-competitive inhibitor with respect to NAD(+). In preliminary experiments, AFA PCB was also effective in reducing the colony formation capacity of PC-3 prostate cancer cells and FTC-133 thyroid cancer cells., Conclusions: Overall, these findings confirmed that AFA and its active principles are natural compounds with high biological activity. Further studies evaluating the effects of AFA PCB in reducing tumor cell growth and phytochemical glucuronidation are encouraged.
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- 2016
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19. Reactive oxygen species a double-edged sword for mesothelioma.
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Benedetti S, Nuvoli B, Catalani S, and Galati R
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- Cell Transformation, Neoplastic metabolism, Humans, Mesothelioma etiology, Mesothelioma pathology, Nanotubes, Carbon adverse effects, Oxidative Stress physiology, Reactive Oxygen Species adverse effects
- Abstract
It is well known that oxidative stress can lead to chronic inflammation which, in turn, could mediate most chronic diseases including cancer. Oxidants have been implicated in the activity of crocidolite and amosite, the most powerful types of asbestos associated to the occurrence of mesothelioma. Currently rates of mesothelioma are rising and estimates indicate that the incidence of mesothelioma will peak within the next 10-15 years in the western world, while in Japan the peak is predicted not to occur until 40 years from now. Although the use of asbestos has been banned in many countries around the world, production of and the potentially hazardous exposure to asbestos is still present with locally high incidences of mesothelioma. Today a new man-made material, carbon nanotubes, has arisen as a concern; carbon nanotubes may display 'asbestos-like' pathogenicity with mesothelioma induction potential. Carbon nanotubes resulted in the greatest reactive oxygen species generation. How oxidative stress activates inflammatory pathways leading to the transformation of a normal cell to a tumor cell, to tumor cell survival, proliferation, invasion, angiogenesis, chemoresistance, and radioresistance, is the aim of this review.
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- 2015
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20. CELLFOOD™ induces apoptosis in human mesothelioma and colorectal cancer cells by modulating p53, c-myc and pAkt signaling pathways.
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Nuvoli B, Santoro R, Catalani S, Battistelli S, Benedetti S, Canestrari F, and Galati R
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- Cell Line, Tumor, Cell Survival drug effects, Colorectal Neoplasms, Cyclin-Dependent Kinase Inhibitor p21 metabolism, Cyclin-Dependent Kinase Inhibitor p27 metabolism, Humans, Mesothelioma, Signal Transduction drug effects, Amino Acids pharmacology, Apoptosis drug effects, Enzymes pharmacology, Minerals pharmacology, Proto-Oncogene Proteins c-akt metabolism, Proto-Oncogene Proteins c-myc metabolism, Sulfates pharmacology, Tumor Suppressor Protein p53 metabolism
- Abstract
Background: CELLFOOD™ (CF) is a nutraceutical non-addictive, non-invasive, and completely non-toxic unique proprietary colloidal-ionic formula. Little is known about its effect on cancer cells in solid tumors. The aim of this study was to evaluate the effect that CF has on different cancer cell lines and the mechanism by which the nutraceutical works., Methods: The effect of CF on HFF (normal fibroblasts), Met5A (mesothelium), MSTO-211H, NCI-2452, Ist-Mes1, MPP89, Ist-Mes2 (mesothelioma), M14 (melanoma), H1650, H1975 (lung cancer), SKRB3 (breast cancer), and HCT-116 (colorectal cancer) cell growth was tested by cell proliferation and clonogenic assay. Among all of them, MSTO-211 and HCT-116 were analyzed for cell cycle by flow cytometry and western blot., Results: All human cancer lines were suppressed on cell growth upon 1:200 CF treatment for 24 and 48 hours. Death was not observed in HFF and Met5A cell lines. Cell cycle analysis showed an increased sub-G1 with reduction of G1 in MSTO-211 and a cell cycle arrest of in G1 in HCT116. Activation of caspase-3 and cleavage of PARP confirmed an apoptotic death for both cell lines. Increased expression levels of p53, p21, and p27, downregulation of c-myc and Bcl-2, and inhibition of Akt activation were also found in CF-treated MSTO-211 and HCT-116 cells., Conclusions: These findings ascertained an interaction between p53, c-myc, p21, p27, Bcl-2, PI3K/Akt pathway, and CF-induced apoptosis in MSTO-211H and HCT-116 cells, suggesting that CF acts as an important regulator of cell growth in human cancer cell lines. CF could be a useful nutraceutical intervention for prevention in colon cancer and mesothelioma.
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- 2014
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21. Metal-on-metal hip prostheses: correlation between debris in the synovial fluid and levels of cobalt and chromium ions in the bloodstream.
- Author
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De Pasquale D, Stea S, Squarzoni S, Bordini B, Amabile M, Catalani S, Apostoli P, and Toni A
- Subjects
- Arthroplasty, Replacement, Hip instrumentation, Female, Follow-Up Studies, Humans, Ions blood, Male, Microscopy, Electron, Scanning, Middle Aged, Prosthesis Failure, Retrospective Studies, Spectrometry, X-Ray Emission, Chromium blood, Cobalt blood, Hip Prosthesis, Metals, Synovial Fluid
- Abstract
Purpose: Hip prostheses with metal-on-metal (MoM) coupling can release cobalt-chromium particles and ions. The aim of this work is to verify the correlation between particles in the synovial fluid and circulating ions., Methods: Forty patients were enrolled; particles from synovial fluid were analysed by SEM–EDX (Scanning Electron Microscopy-Energy Dispersion X-rays analysis) and levels of circulating Co and Cr were assayed by ICP-MS (inductively-coupled plasma mass spectrometry)., Results: In 16 cases we did not find any particles in the synovial fluid and the Co level in whole blood was 0.05–4.42 ppb; in seven with few particles the blood level was 2.2–15.6 ppb; in six cases with several particles the level was 5.0–54.3 ppb; finally, in 11 cases we isolated not only Co-Cr particles, but also Cr particles with low or absent Co and in these patients the circulating level of Co was 23.8–109.6 ppb. Co in serumand Cr level both whole blood and serum have shown a similar trend to Co; the correlation between all these values and the corresponding particles is statistically significant in all cases., Conclusion: Co and Cr both in serum and whole blood represents a systemic representation of the particle release at local level and can therefore be used to confirm a diagnosis and monitor the wear process of MoM articular prostheses.
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- 2014
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22. Metabolism modifications and apoptosis induction after Cellfood™ administration to leukemia cell lines.
- Author
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Catalani S, Carbonaro V, Palma F, Arshakyan M, Galati R, Nuvoli B, Battistelli S, Canestrari F, and Benedetti S
- Subjects
- Amino Acids pharmacology, Apoptosis drug effects, Cell Growth Processes physiology, Cell Hypoxia physiology, Cell Line, Tumor, Enzymes pharmacology, Humans, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Jurkat Cells, K562 Cells, Leukemia pathology, Minerals pharmacology, Rhodophyta chemistry, Sulfates pharmacology, U937 Cells, Dietary Supplements, Leukemia drug therapy, Leukemia metabolism, Plant Extracts pharmacology
- Abstract
Background: Cellfood™ (CF) is a nutritional supplement containing deuterium sulphate, minerals, amino acids, and enzymes, with well documented antioxidant properties. Its organic and inorganic components are extracted from the red algae Lithothamnion calcareum, whose mineral extract has shown growth-inhibitory effect both on in vitro and in vivo models. The purpose of this study was to evaluate the antiproliferative effects of CF on leukemic cells. In fact, according to its capacity to modulate O2 availability and to improve mitochondrial respiratory metabolism, we wondered if CF could affect cancer cell metabolism making cells susceptible to apoptosis., Methods: Three leukemic cell lines, Jurkat, U937, and K562, were treated with CF 5 μl/ml up to 72 hours. Cell viability, apoptosis (i.e. caspase-3 activity and DNA fragmentation), hypoxia inducible factor 1 alpha (HIF-1α) concentration, glucose transporter 1 (GLUT-1) expression, lactate dehydrogenase (LDH) activity and lactate release in the culture medium were detected and compared with untreated cells., Results: CF significantly inhibited leukemic cell viability by promoting cell apoptosis, as revealed by caspase-3 activation and DNA laddering. In particular, CF treated cells showed lower HIF-1α levels and lower GLUT-1 expression as compared to untreated cells. At the same time, CF was able to reduce LDH activity and, consequently, the amount of lactate released in the extracellular environment., Conclusions: We supplied evidence for an antiproliferative effect of CF on leukemia cell lines by inducing cell death through an apoptotic mechanism and by altering cancer cell metabolism through HIF-1α and GLUT-1 regulation. Thanks to its antioxidative and proapoptotic properties, CF might be a good candidate for cancer prevention.
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- 2013
- Full Text
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23. The antioxidant protection of CELLFOOD against oxidative damage in vitro.
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Benedetti S, Catalani S, Palma F, and Canestrari F
- Subjects
- DNA metabolism, Erythrocytes metabolism, Glutathione metabolism, Humans, Lymphocytes metabolism, Antioxidants pharmacology, Dietary Supplements, Oxidative Stress
- Abstract
CELLFOOD (CF) is an innovative nutritional supplement containing 78 ionic/colloidal trace elements and minerals combined with 34 enzymes and 17 amino acids, all suspended in a solution of deuterium sulfate. The aim of this study was to investigate, for the first time, the antioxidant properties of CF in vitro in different model systems. Three pathophysiologically relevant oxidants were chosen to evaluate CF protection against oxidative stress: hydrogen peroxide, peroxyl radicals, and hypochlorous acid. Both biomolecules (GSH and plasmid DNA) and circulating cells (erythrocytes and lymphocytes) were used as targets of oxidation. CF protected, in a dose-dependent manner, both GSH and DNA from oxidation by preserving reduced GSH thiol groups and supercoiled DNA integrity, respectively. At the same time, CF protected erythrocytes from oxidative damage by reducing cell lysis and GSH intracellular depletion after exposure to the oxidant agents. In lymphocytes, CF reduced the intracellular oxidative stress induced by the three oxidants in a dose-dependent manner. The overall in vitro protection of biomolecules and cells against free radical attacks suggests that CF might be a valuable coadjuvant in the prevention and treatment of various physiological and pathological conditions related to oxidative stress, from aging to atherosclerosis, from neurodegeneration to cancer., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2011
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24. Multielemental analysis of tissues from Cangrande della Scala, Prince of Verona, in the 14th century.
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Apostoli P, De Palma G, Catalani S, Bortolotti F, and Tagliaro F
- Subjects
- Adult, Autopsy, Hair chemistry, History, Medieval, Humans, Italy, Liver chemistry, Mass Spectrometry, Arsenic Poisoning diagnosis, Arsenic Poisoning history, Elements, Metals analysis
- Abstract
Cangrande della Scala, Prince of Verona (Italy), died suddenly shortly after his triumph in the battle of Treviso (July 18, 1329). Thus, in the frame of a multidisciplinary paleo-pathological study, we carried out a multielemental analysis on the Prince's tissue specimens, including hair, liver, muscle, and bone, in order to characterize a multitissue profile of metallic elements in a nobleman of the 14th century. Biological specimens were analyzed by inductively coupled plasma-mass spectrometry. We were able to rule out arsenic poisoning as the primary cause of death. High levels of gold and silver in both hair and liver samples were probably due to prolonged contact of the mummy with precious metals in the funeral garments. High lead concentrations in both liver and bone tissue can be traced back to the ingestion of contaminated food and alcoholic beverages. Most of the essential elements were in the normal range of values for contemporary living people. The low arsenic and chromium levels in the Prince's tissues as compared to modern people would be suggestive of raised concentrations of both the elements in the present era, which are likely due to industrial pollution.
- Published
- 2009
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25. Metallic elements in lung tissues: results of a meta-analysis.
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Catalani S, De Palma G, Mangili A, and Apostoli P
- Subjects
- Humans, Lung chemistry, Lung Neoplasms chemistry, Metals analysis
- Abstract
Several studies have investigated the levels of metallic elements in the pulmonary tissues of healthy subjects, patients with lung diseases and occupationally exposed subjects. The present meta-analysis was aimed at both assessing the possible contribution of metal exposure to the development of lung diseases, including lung cancer, and evaluating systematically the role and the weight of variability factors affecting the results of such studies. A literature research covering the period 1980-2007 was conducted using the public database PubMed. A standard scoring method was elaborated with a minimum score of 5 for inclusion and evaluation. Selected papers underwent a meta-analytical assessment. Fifty-eight papers were retrieved, but 21 of them could not be admitted to further analysis, due to failure to achieve the minimum score. The main limitations of individual studies included: limited sample sizes, poor control of smoking habits and differences in subjects' ages, lung tissue topography, sampling methods, storage procedures and data analysis. Copper and zinc were the most represented elements (121.96 +/- 0.74 and 12.98 +/- 0.07 microg/g dry weight, respectively). Among toxic metals, the highest concentrations were observed for chromium and lead (2.42 +/- 0.12 and 2.14 +/- 0.04 microg/g, respectively). Tissue concentrations were similar in unaffected tissues from both controls and lung cancer patients, whereas they were lower in lung tumor samples. A considerable intra- and inter-individual variability was noted. Such a variability of measures, combined with the very low metal concentrations calls for the definition and use of standardized procedures of sample collection, storage, and analysis.
- Published
- 2008
26. Genotoxic and oxidative effects induced on A549 cells by extract of PM10 collected in an electric steel plant.
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Cavallo D, Ursini CL, Maiello R, Apostoli P, Catalani S, Ciervo A, and Iavicoli S
- Subjects
- Carcinogenicity Tests, Cells, Cultured, Humans, Occupational Exposure, Steel, DNA Damage, Lung cytology, Metallurgy, Oxidative Stress, Particulate Matter toxicity, Respiratory Mucosa cytology
- Abstract
The present study was aimed at assessing the carcinogenic risk of occupational exposure to PM10 in electric steel plants. PM10 was collected on cellulose filter respectively outside (site 1) and inside (site 2) the furnace area, was measured, extracted and its metal content was analysed by ICP-MS. Cells were exposed for 30 min, 2 and 4 hours to extract of filter from each site diluted at 0.004, 0.008 and 0.02%. The direct/oxidative DNA damage caused by PM10 was evaluated on A549 cells by Fpg-modified comet assay, analysing Tail moment (TM) and comet percentage. Air samples contained 1.08 mg/m3 of PM10 in site 1 and 5.54 mg/m3in site 2 and different amounts of metals with higher levels of Zn, Al, Ni, Pb, Cd, Cr, Ba in site 2 and of Fe, Mn, Sb in site 1. In cells exposed for 2h to PM10 from both sites, an oxidative DNA damage was found concentrations of 0.008% and 0.02%. For site 2, a direct DNA damage at 0.02% was also found. After 4h a direct/oxidative DNA damage was detected at 0.02% for site 2 and an oxidative DNA damage for site 1. The results indicate a moderate DNA damage induction by used diluitions of PM10 extracts with higher extent for more polluted site 2. These findings show the suitability of this experimental model to evaluate early DNA damage induced by complex mixtures containing metals on target organ, suggesting its use to study biological effects of occupational exposure to such substances.
- Published
- 2008
27. Metallic elements in pulmonary biopsies from lung cancer and control subjects.
- Author
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De Palma G, Goldoni M, Catalani S, Carbognani P, Poli D, Mozzoni P, Acampa O, Internullo E, Rusca M, and Apostoli P
- Subjects
- Adult, Aged, Biopsy, Case-Control Studies, Female, Humans, Male, Middle Aged, Carcinoma, Non-Small-Cell Lung chemistry, Carcinoma, Non-Small-Cell Lung pathology, Lung Neoplasms chemistry, Lung Neoplasms pathology, Metals analysis
- Abstract
Occupational/environmental exposure to some metallic elements is a risk factor for the development of lung diseases, including lung cancer. We aimed at investigating the levels of arsenic, beryllium, cadmium, cobalt, chromium, nickel and lead in the lung tissue of patients affected by early stage non small cell lung cancer (NSCLC). A small number of patients without a diagnosis of lung cancer were also included as control group. Lung tissue biopsies were collected from 45 NSCLC patients (both cancerous and unaffected tissues) and 8 control subjects undergoing surgery. Patients were stratified for smoking habits, histopathology and cancer sites. Metallic elements were determined in dry tissue after digestion by means of ICP-MS. Cd, Ni and Pb levels were higher in unaffected than in control tissues (0.52 vs 0.18 microg/g dry, p < 0.05 for Cd; 4.49 vs 1.8 microg/g dry,p < 0.05 for Ni; 0.21 vs 0.06 microg/g dry, p < 0.01 for Pb). The three elements, and particularly Cd, were influenced by smoking habits; Pb levels were higher in squamocellular carcinoma than adenocarcinomas; Ni distributed in the lungs in an inhomogeneous way. This study demonstrates that the unaffected lung tissue is more representative than the cancerous tissue of the pulmonary content of metallic elements. Tobacco smoke is a main factor affecting the concentration levels of Cd, Pb, and to a lesser extent Ni in the lung tissues of NSCLC patients. The role of past environmental-occupational exposures could not be fully elucidated, due to the limited sample size and the retrospective nature of the study.
- Published
- 2008
28. Exhaled breath condensate as a suitable matrix to assess lung dose and effects in workers exposed to cobalt and tungsten.
- Author
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Goldoni M, Catalani S, De Palma G, Manini P, Acampa O, Corradi M, Bergonzi R, Apostoli P, and Mutti A
- Subjects
- Adult, Case-Control Studies, Cobalt urine, Environmental Monitoring, Female, Humans, Male, Oxidative Stress, Sensitivity and Specificity, Tungsten urine, Breath Tests, Cobalt analysis, Occupational Exposure, Tungsten analysis
- Abstract
The aim of the present study was to investigate whether exhaled breath condensate (EBC), a fluid formed by cooling exhaled air, can be used as a suitable matrix to assess target tissue dose and effects of inhaled cobalt and tungsten, using EBC malondialdehyde (MDA) as a biomarker of pulmonary oxidative stress. Thirty-three workers exposed to Co and W in workshops producing either diamond tools or hard-metal mechanical parts participated in this study. Two EBC and urinary samples were collected: one before and one at the end of the work shift. Controls were selected among nonexposed workers. Co, W, and MDA in EBC were analyzed with analytical methods based on mass spectrometric reference techniques. In the EBC from controls, Co was detectable at ultratrace levels, whereas W was undetectable. In exposed workers, EBC Co ranged from a few to several hundred nanomoles per liter. Corresponding W levels ranged from undetectable to several tens of nanomoles per liter. A parallel trend was observed for much higher urinary levels. Both Co and W in biological media were higher at the end of the work shift in comparison with preexposure values. In EBC, MDA levels were increased depending on Co concentration and were enhanced by coexposure to W. Such a correlation between EBC MDA and both Co and W levels was not observed with urinary concentration of either element. These results suggest the potential usefulness of EBC to complete and integrate biomonitoring and health surveillance procedures among workers exposed to mixtures of transition elements and hard metals. Key words: cobalt, exhaled breath condensate, hard metals, lung, malondialdehyde, oxidative stress, tungsten.
- Published
- 2004
- Full Text
- View/download PDF
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