1. O13.2 Molecular epidemiology in relation to azithromycin resistance inneisseria gonorrhoeaeisolates from amsterdam, the netherlands, between 2008 and 2015 – a case-control study
- Author
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Mirjam Dierdorp, Caroline Wind, Maarten F. Schim van der Loeff, Sylvia M. Bruisten, Henry J. C. de Vries, and Alje P. van Dam
- Subjects
Molecular epidemiology ,Biology ,Azithromycin ,medicine.disease_cause ,Virology ,Men who have sex with men ,Microbiology ,Minimum inhibitory concentration ,23S ribosomal RNA ,Ceftriaxone ,medicine ,Neisseria gonorrhoeae ,Typing ,medicine.drug - Abstract
Neisseria gonorrhoeae resistance to ceftriaxone and azithromycin increases, which threatens the recommended dual therapy based on these antimicrobials. We used molecular epidemiology to identify N. gonorrhoeae clusters, and associations with azithromycin resistance in Amsterdam, the Netherlands. N. gonorrhoeae isolates were selected from patients visiting the Amsterdam Sexually Transmitted Infections Clinic, from January 2008 through September 2015. We included all azithromycin resistant isolates (minimum inhibitory concentration [MIC] ≥2.0 mg/L), and frequency matched susceptible controls (MIC ≤0.25 mg/L). All isolates were tested using 23S rRNA sequencing, N. gonorrhoeae multiantigen sequence typing (NG-MAST), and multilocus variable-number of tandem repeat analysis (NG-MLVA). A hierarchical cluster analysis of NG-MLVA related to resistance and epidemiological characteristics was performed. We analysed 143 isolates (69 resistant and 74 susceptible); 81% was from men who have sex with men (MSM). Azithromycin resistant isolates had significantly more often C2611T mutations of 23S rRNA (n=62, 89.9%, p
- Published
- 2017
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