37 results on '"Cardoso, Rebeca"'
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2. Intestinal helminth infection transforms the CD4+ T cell composition of the skin
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Classon, Cajsa H., Li, Muzhen, Clavero, Ada Lerma, Ma, Junjie, Feng, Xiaogang, Tibbitt, Christopher A., Stark, Julian M., Cardoso, Rebeca, Ringqvist, Emma, Boon, Louis, Villablanca, Eduardo J., Rothfuchs, Antonio Gigliotti, Eidsmo, Liv, Coquet, Jonathan M., and Nylén, Susanne
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- 2022
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3. Liver X receptor regulates Th17 and RORγt+ Treg cells by distinct mechanisms
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Parigi, Sara M., Das, Srustidhar, Frede, Annika, Cardoso, Rebeca F., Tripathi, Kumar Parijat, Doñas, Cristian, Hu, Yue O. O., Antonson, Per, Engstrand, Lars, Gustafsson, Jan-Åke, and Villablanca, Eduardo J.
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- 2021
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4. Intestinal damage is required for the pro-inflammatory differentiation of commensal CBir1-specific T cells
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Sorini, Chiara, primary, Cardoso, Rebeca F., additional, Tripathi, Kumar P., additional, Mold, Jeff E., additional, Diaz, Oscar E., additional, Holender, Yael, additional, Kern, Bianca C., additional, Czarnewski, Paulo, additional, Gagliani, Nicola, additional, and Villablanca, Eduardo J., additional
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- 2023
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5. PROCESSO TRANSEXUALIZADOR NO ESTADO DO CEARÁ
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De Oliveira Silva, Felipe, primary, Cortês Barcelos, Suzyane, additional, Almeida Melo, Ana Luísa, additional, José Soares Pontes, Ricardo, additional, and De Oliveira Cardoso, Rebeca, additional
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- 2022
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6. Beyond Th1 and Treg : intestinal T helper cells in disease and tolerance
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Cardoso, Rebeca Franco and Cardoso, Rebeca Franco
- Abstract
The human intestinal tract is not only one of the largest organs by surface area in our bodies but also harbors the greatest number of immune cells. These cells are crucial for maintaining tolerance towards the various food and microbial antigens and mounting protective inflammatory responses towards invading pathogens. Inasmuch, a delicate balance exists between anti- and pro-inflammatory signals which are influenced by several factors and must remain in check to ensure appropriate responses towards innocuous and harmful stimuli. To this end, CD4+ T helper (Th) cells play a crucial role in orchestrating both tolerogenic and proinflammatory responses. This thesis sought to better understand the factors controlling Th cells in the context of different intestinal challenges. In Studies I and II, we investigated how dietary cholesterol sensing can influence Th phenotypes during homeostasis. In Study I, we found that the liver X receptors (LXRs)— which senses oxidized forms of cholesterol—influence the balance of Foxp3+RORgt+ regulatory T cells (RORgt+ Tregs) and Foxp3-RORgt+ Th17 cells in the gut-draining mesenteric lymph nodes (MLNs). Differences in the microbiota could explain the changes in Th17 frequencies but not RORgt+ Tregs. Instead, we found that LXR signaling in CD11c+ cells altered the RORgt+ Treg population. In Study II, we found that ingestion of the LXR agonist GW3965 led to changes in the frequencies in the IL-10+ Foxp3+ Treg populations in the small intestine and spleen. Interestingly, while GW3965 led to a decrease in IL-10+ Tregs the small intestine, it decreased their frequency in the spleen. Intriguingly, mice lacking LXR signaling in CD11c+ cells failed to develop oral tolerance. Together, these studies demonstrate the importance of cholesterol sensing during steady state. In Study III, we tracked the activation of naïve Th cells recognizing the commensal CBir1 antigen (CBir1 cells). We found that naïve CBir1 cells could be activated in the MLNs fol
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- 2022
7. Performance supplementation and ingestive behavior of sheep finished in continuous pasture in the period of water restriction
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Cardoso, Rebeca Rocha, primary, Sousa, Luciano Fernandes, additional, Holanda, Ana Cristina Ferreira, additional, and Bentes, Glenda Neves, additional
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- 2022
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8. Análisis de la eficacia de los sorbentes lignocelulósicos naturales en la remediación de hidrocarburos vertidos en aguas marinas utilizando fibra de sisal
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Cardoso, Rebeca da Paixão Gomes, Cavalcante, Bárbara Milla Nogueira, Brioude, Michel de Meireles, and Moreira, Ícaro Thiago Andrade
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Adsorção ,Derrame de petróleo ,Adsorption ,Sisal fiber ,Derramamento de petróleo ,Adsorción ,Fibra de sisal ,Oil spill - Abstract
Oil production, applied in various sectors of the economy, can trigger environmental impacts from marine spills and spills in exploration, transportation and storage, capable of destroying biomes. To remedy impacted areas, containment and cleaning barriers in a marine environment are used. Lignocellulosic fibers have a high capacity to absorb oil, and can easily serve as barriers in a spill process, preventing the oil stain from spreading, in addition to providing remediation of the impacted site. Sisal fiber is a lignocellulosic material and is found on a large scale in nature as a by-product of agricultural production in addition to being materials from renewable sources as well as biodegradable. The present work aims to carry out a study involving tests carried out in the laboratory in order to verify the efficiency of fresh sisal fiber in the sorption of oil in the Campos Basin. The morphological analysis of the fiber, through the Scanning Electron Microscope (SEM), allowed access to enlarged images of the surface area of the fiber, composed of pores responsible for the oil adsorption process. To assess its efficiency, offshore spill tests were simulated, under different periods (5, 20, 40, 60, 90 and 120 minutes), using the reciprocating table (126 rpm), which simulates marine hydrodynamics. The data collected suggested the need for a minimum time of 5 minutes of contact with oil in eventual marine spills. Fiber recycling was analyzed over six subsequent cycles. The adsorptive potential of the last cycle dropped approximately 12% from the first sorption, characterizing good fiber reuse after successive applications. La producción de petróleo, aplicada en varios sectores de la economía, puede desencadenar impactos ambientales por derrames marinos y derrames en exploración, transporte y almacenamiento, capaces de destruir biomas. Para remediar las áreas impactadas, se utilizan barreras de contención y limpieza en un ambiente marino. Las fibras lignocelulósicas tienen una alta capacidad para absorber aceite y pueden servir fácilmente como barreras en un proceso de derrame, evitando que la mancha de aceite se propague, además de proporcionar remediación del sitio impactado. La fibra de sisal es un material lignocelulósico y se encuentra a gran escala en la naturaleza como subproducto de la producción agrícola, además de ser materiales de fuentes renovables y biodegradables. El presente trabajo tiene como objetivo realizar un estudio de ensayos realizados en laboratorio para verificar la eficiencia de la fibra fresca de sisal en la sorción de aceite en la Cuenca de Campos. El análisis morfológico de la fibra, a través del Microscopio Electrónico de Barrido (SEM), permitió acceder a imágenes ampliadas de la superficie de la fibra, compuesta por poros responsables del proceso de adsorción del aceite. Para evaluar su eficiencia, se simularon pruebas de derrames costa afuera, en diferentes períodos (5, 20, 40, 60, 90 y 120 minutos), utilizando la mesa recíproca (126 rpm), que simula la hidrodinámica marina. Los datos recopilados sugirieron la necesidad de un tiempo mínimo de 5 minutos de contacto con el petróleo en eventuales derrames marinos. El reciclaje de fibra se analizó durante seis ciclos posteriores. El potencial adsortivo del último ciclo disminuyó aproximadamente un 12% desde la primera sorción, caracterizando una buena reutilización de la fibra luego de sucesivas aplicaciones. A produção do petróleo, aplicados em diversos setores da economia, pode desencadear impactos ambientais provenientes de derrames e vazamentos marinhos na exploração, transporte e armazenamento, capazes de destruir biomas. Para remediar áreas impactadas, são utilizadas barreiras de contenção e limpeza em ambiente marinho. As fibras lignocelulósicas apresentam uma alta capacidade de sorver o óleo, podendo facilmente servir como barreiras em um processo de derramamentos, evitando que a mancha de óleo se alastre, além de proporcionar a remediação do local impactado. A fibra de sisal é um material lignocelulósico e é encontrada em larga escala na natureza como subproduto de produções agrícolas além de serem materiais de fontes renováveis bem como biodegradáveis. O presente trabalho apresenta, como objetivo, realizar um estudo envolvendo testes realizados em laboratório a fim de verificar a eficiência da fibra de sisal in natura na sorção do petróleo da Bacia de Campos. As análises morfológicas da fibra, através do Microscópio Eletrônico de Varredura (MEV), permitiram o acesso a imagens ampliadas da área superficial da fibra, compostas por poros responsáveis pelo processo de adsorção do óleo. Para avaliar a sua eficiência, ensaios de derramamentos offshore foram simulados, sob períodos distintos (5, 20, 40, 60, 90 e 120min), utilizando a mesa reciprocante (126 rpm), que simula a hidrodinâmica marinha. Os dados colhidos sugeriram a necessidade de um tempo mínimo de 5 minutos de contato com óleo em eventuais derramamentos marinhos. A reciclagem da fibra foi analisada ao longo de seis ciclos subsequentes. O potencial adsortivo do último ciclo decaiu aproximadamente 12% da primeira sorção, caracterizando uma boa reutilização da fibra após sucessivas aplicabilidades.
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- 2021
9. Health perspectives of women with breast cancer
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Leite Cardoso, Rebeca, primary, Baumgratz de Paula, Patrícia Aparecida, additional, and Teixeira Vaz, Camila, additional
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- 2021
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10. A committed tissue-resident memory T cell precursor within the circulating CD8+ effector T cell pool
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Kok, Lianne, primary, Dijkgraaf, Feline E., additional, Urbanus, Jos, additional, Bresser, Kaspar, additional, Vredevoogd, David W., additional, Cardoso, Rebeca F., additional, Perié, Leïla, additional, Beltman, Joost B., additional, and Schumacher, Ton N., additional
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- 2020
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11. Intestinal helminth infection transforms the CD4+ T cell composition of the skin.
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Classon, Cajsa H., Li, Muzhen, Clavero, Ada Lerma, Ma, Junjie, Feng, Xiaogang, Tibbitt, Christopher A., Stark, Julian M., Cardoso, Rebeca, Ringqvist, Emma, Boon, Louis, Villablanca, Eduardo J., Rothfuchs, Antonio Gigliotti, Eidsmo, Liv, Coquet, Jonathan M., and Nylén, Susanne
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- 2022
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12. Performance supplementation and ingestive behavior of sheep finished in continuous pasture in the period of water restriction.
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Rocha Cardoso, Rebeca, Fernandes Sousa, Luciano, Ferreira Holanda, Ana Cristina, and Neves Bentes, Glenda
- Abstract
The objective was to evaluate the performance and ingestive behavior of grazing sheep in the finishing stage, with supplementation in the period of water restriction. Fifteen male crossbred sheep were used in continuous pasture in Massai grass and supplied supplement formulated with 18% of crude protein in three levels (0.0; 0.3 and 0.6% of body weight), individually. Bromatological and fodder production analyses were performed, as well as foliar mass production, stem mass and senescent material mass. The performance and ingestive behavior of the animals were evaluated. The design was in four randomized blocks for the variables measured in the fodder. For the biometry, weight gain and behavior variables measured in the animals, the design was entirely randomized with five repetitions. The total forage mass production was 5512.41 kg ha-1, with 6.58% of crude protein, 79 38% of neutral detergent fiber and with 65% of foliar mass. The total weight gain and daily weight gain were higher in animals that received a supplement of 0.6% of body weight. In general, the animals grazed more in the morning period and the supplemented ones destined more time for rumination and leisure than the ones not supplemented. [ABSTRACT FROM AUTHOR]
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- 2022
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13. Ingestive behavior of sheep on Massai grass under different pre-grazing heights under rotational stocking
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CARDOSO, Rebeca Rocha, SOUSA, Luciano Fernandes de, FERREIRA, Ana Cristina Holanda, NEIVA, José Neuman Miranda, FERREIRA, Deborah Alves, and SILVA, André Guimarães Maciel e
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ruminants ,Megathyrsus maximus ,ruminantes ,Panicum maximum - Abstract
SUMMARY This studyaimed to assess the influence of three pre-grazing heights of Massai grass on the ingestive behavior of sheep under rotational stocking. Eighteen Dorper x nondescript crossbred sheep were used under rotational stocking on Massai grass at pre-grazing heights. The animal stocking was estimated to ensure a reduction of approximately 50% of the initial height. Behavior assessments were performed from 6 to 18 h at the entrance and exit of animals from paddocks. The longest activity was grazing, followed by rumination and other activities, and the period from 6 to 10 h showed the longest grazing.In the other periods, the animals in the 35-cm treatment grazed less when compared to those ofthe other treatments. Rumination in all treatments was more significant in the period from 14 to 18 h, but the animals in the 45-cm treatment spent more time doing this activity, which gave them a higher time of rumination of the food bolus, as well as ruminating chews. The bite rate was higher for the 25-cm treatment, followed by the 45 and 35-cm treatments, respectively. RESUMO O objetivo foi avaliar a influência de três alturas pré-pastejo do capim Massai sobre o comportamento ingestivo de ovinos sob lotação rotativa. Foram utilizados 18 ovinos mestiços Dorper x sem padrão racial definido (SPRD) sob lotação rotativa em capim Massai nas alturas pré-pastejo de 45, 35 e 25 cm. A lotação animal foi estimada para garantir a redução de aproximadamente 50% da altura inicial. As avaliações de comportamento foram feitas das 6 às 18 h na entrada e na saída dos animais dos piquetes. A maior atividade de forma geral realizada foi a de pastejo, seguida de ruminação e outras atividades, sendo o período de 6 às 10 h o que apresentou maior atividade de pastejo. Nos demais períodos, os animais no tratamento com 35 cm pastejaram menos que os animais dos outros tratamentos. A ruminação, em todos os tratamentos, foi mais significativa no período das 14 às 18 h, mas os animais no tratamento com 45 cm foram os que passaram maior tempo realizando esta atividade. Os animais no tratamento com 45 cm apresentaram maior tempo de ruminação do bolo alimentar e maior número de mastigações merícicas. A taxa de bocados foi superior para o tratamento com 25 cm, seguido do com 45 e 35 cm respectivamente.
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- 2019
14. Altura pré-pastejo para o capim massai sob lotação rotativa com ovinos no trópico úmido brasileiro no período chuvoso
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Cardoso, Rebeca Rocha, Sousa, Luciano Fernandes, and Ferreira, Ana Cristina Holanda
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Megathyrsus maximus ,Panicum maximum ,Ruminantes ,Morfogênese ,Bromatológica ,Comportamento ,Ruminants ,Morphogenesis ,Bromatological ,Behavior ,CIENCIAS AGRARIAS::ZOOTECNIA [CNPQ] - Abstract
O objetivo foi avaliar altura de entrada dos animais adequada à fisiológia da planta e ao desempenho de ovinos de corte em pastagem de capim Massai. Foram utilizados 18 ovinos mestiços Dorper x sem padrão racial definido (SPRD) pastejando em três alturas pré-pastejo em capim Massai, sendo as alturas de 45 cm, 35 cm e 25 cm. Foi adotado o método de lotação rotativa com período fixo de 12 e 4 dias de descanso e de ocupação respectivamente. Antes da entrada dos animais na área foi estimada a lotação animal necessária para garantir a redução de aproximadamente 50% da altura inicial. Foram obtidos o valor de altura real, a produtividade do capim Massai e a composição estrutural da planta e, por fim, tais variáveis foram correlacionadas. Avaliou-se as características morfogênicas, a composição bromatológica da forragem e o desempenho dos animais. As observações de comportamento ingestivo foram feitas das 6 h às 18 h no segundo e no terceiro ciclo de avaliação, na entrada e na saída dos animais dos piquetes, somando quatro dias de observação. Também foram observados os tempos de pastejo, de ruminação e de outras atividades. Nesse quesito, essas observações foram agrupadas e analisadas em três períodos do dia, 6 h às 10 h, 10 h às 14 h e 14 h às 18 h. Foram contabilizados o tempo para realizar bocados, o tempo de ruminação do bolo alimentar, o número de mastigações merícicas e a taxa de bocados e agrupados nos períodos de entrada e saída dos animais da área. O tratamento com 35 cm apresentou maior densidade de forragem, maiores produções de massa seca total, de lâmina foliar e de forragem morta, maior quantidade de forragem disponível, maior oferta de forragem e maior taxa de acúmulo diária. O tratamento com 25 cm apresentou maior relação folha:colmo e maior porcentagem de folhas na estrutura da forrageira. Por sua vez, o tratamento com 45 cm apresentou maiores porcentagens de colmo e de forragem morta, acarretando uma menor apreensão de folhas pelos animais. A taxa de aparecimento de folhas, a severidade de desfolhação e o número de folhas vivas foram maiores para o tratamento com 25 cm, não diferindo do tratamento com 35 cm nesta última variável. A altura de 25 cm reduziu o alongamento de colmo, o filocrono e o comprimento da bainha. Os maiores teores de proteína e nutrientes digestíveis totais foram observados no tratamento com 35 cm seguido do tratamento com 25 cm. O maior teor de fibra em detergente ácido foi observado no tratamento com 45 cm. O maior consumo foi obtido pelo tratamento com 35 cm, assim como a maior taxa de lotação que desencadeou o maior ganho de peso por área. A maior atividade de comportamento realizada foi a de pastejo, seguida de ruminação e outras atividades, sendo o período de 6 h às 10 h o que apresentou maior atividade de pastejo. Nos demais períodos do dia os animais no tratamento com 35 cm pastejaram menos que os animais dos outros tratamentos. A ruminação, em todos os tratamentos, foi mais significativa no período das 14 h às 18 h, mas os animais no tratamento com 45 cm foram os que passaram maior tempo realizando esta atividade e tiveram menos tempo para outras atividades. Os animais no tratamento com 45 cm apresentaram maior tempo de ruminação do bolo alimentar e maior número de mastigações merícicas. A taxa de bocados foi superior para o tratamento com 25 cm, seguido do com 45 cm e 35 cm respectivamente, o inverso aconteceu para o tempo necessário para efetuar os bocados. Com base nos resultados apresentados, a altura de 35 cm, entre as alturas de pré-pastejo avaliadas, é a mais indicada para o capim Massai utilizado na criação de ovinos de corte a pasto, por apresentar maior disponibilidade de alimento, com uma boa qualidade da forragem e por possibilitar maior ganho de peso dos animais por área. The objective was to evaluate the height of entry of the animals adequate to the physiology of the plant and to the performance of sheep in the pasture of Massai grass. We used 18 Dorper x crossbred sheep with no defined racial pattern (SPRD) grazing at three pre-grazing heights in Massai grass, being heights of 45 cm, 35 cm and 25 cm. The rotational grazing method was adopted with a fixed period of 12 and 4 days of rest and occupation, respectively. Prior to entry of the animals into the area, the animal stocking rate was estimated to be approximately 50% of the initial height. The values of real height, the productivity of the Massai grass and the structural composition of the plant were obtained and, finally, these variables were correlated. The morphogenic characteristics, the forage composition and the performance of the animals were evaluated. Ingestive behavior observations were made from 6 h to 18 h in the second and third evaluation cycles, at the entrance and exit of the animals from the pickets, adding four days of observation. Grazing times, rumination times and other activities were also observed. In that question, these observations were grouped and analyzed in three periods of the day, from 6 h am to 10 h a.m., 10 h a.m. to 2 h p.m. and 14 h a.m. to 6 h p.m. The time to perform bites, the time of rumination of the food bolus, the number of merciful chews and the bit rate and grouped in the periods of entry and exit of the animals of the area were counted. The treatment with 35 cm showed higher forage density, higher yields of total dry mass, leaf blade and dead fodder, greater amount of forage available, greater forage supply and higher rate of daily accumulation. The treatment with 25 cm showed a higher leaf: stem ratio and a higher percentage of leaves in the forage structure. On the other hand, the treatment with 45 cm presented higher percentages of stalk and dead forage, resulting in less seizure of leaves by the animals. Leaf appearance rate, defoliation severity and number of live leaves were higher for the 25 cm treatment, not differing from the 35 cm treatment in the latter variable. The height of 25 cm reduced stem elongation, phyllochron, and sheath length. The highest levels of protein and total digestible nutrients were observed in the treatment with 35 cm followed by treatment with 25 cm. The highest acid detergent fiber content was observed in the 45 cm treatment. The highest consumption was obtained by treatment with 35 cm, as well as the higher stocking rate that triggered the highest weight gain per area. The highest behavioral activity was grazing, followed by rumination and other activities, and the period from 6 h a.m. to 10 h a.m. showed the highest grazing activity. At the other periods of the day the animals in the treatment with 35 cm grazed less than the animals of the other treatments. Rumination in all treatments was more significant in the period from 2 h p.m. to 6 h p.m., but the animals in the treatment with 45 cm were the ones that spent more time doing this activity and had less time for other activities. The animals in the treatment with 45 cm presented greater time of rumination of the food bolus and more number of mericics chews. The bite rate was higher for the 25 cm treatment, followed by the 45 cm and 35 cm treatment respectively, the inverse occurred for the time needed to make the bites. Based on the results presented, the height of 35 cm, among the pre-grazing heights evaluated, is the best indicated for the Massai grass used in the rearing of sheep from grass to pasture, due to the greater availability of food, with a good quality and for allowing greater weight gain of the animals per area.
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- 2018
15. ERROS EM RECEITAS DE CONTROLE ESPECIAL E NOTIFICAÇÕES DE RECEITA PRESCRITAS POR PROFISSIONAIS NÃO MÉDICOS
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Cardoso, Rebeca Santos, Mota, Ionara Vieira Rocha da, Lemos, Lucas Brasileiro, Almeida, Paulo Henrique Ribeiro Fernandes, Rosa, Mário Borges, and Lemos, Gisele da Silveira
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medication errors ,odontólogos ,Drug prescription ,erros de medicação ,veterinarians ,dentists ,Prescrição de medicamentos ,médicos-veterinários - Abstract
Introduction: Dental surgeons and veterinary doctors are legally entitled to prescribe drugs under special control with indication within their areas of practice. However, there is a gap in studies that describe the completeness of prescriptions performed by non-medical professionals according to laws and health and ethical standards established in Brazil. Objective: To evaluate errors in prescription notifications and white prescriptions of drugs subject to special control by Portaria 344/1998, which have been performed by non-medical professionals, dental surgeons, and veterinarians. Methodology: A cross-sectional study, based on documentary analysis of prescriptions that were filed in a community pharmacy from a municipality in the state of Bahia, with the application of a standardized form. The EpiData 3.1 and SPSS 21.0 software helped to tabulate and analyze the data, described in absolute and relative frequency and with mean and standard deviation of significance level p
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- 2018
16. Short-term evaluation of Massai grass forage yield and agronomic characteristics and sheep performance under rotational grazing with different pre-grazing canopy heights
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Cardoso, Rebeca Rocha, primary, Sousa, Luciano Fernandes, additional, Ferreira, Ana Cristina Holanda, additional, Neiva, José Neuman Miranda, additional, Ferreira, Deborah Alves, additional, Silva, André Guimarães Maciel e, additional, Nóbrega, Elcivan Bento da, additional, and Silva, Thais Valéria Souza, additional
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- 2019
- Full Text
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17. Ingestive behavior of sheep on Massai grass under different pre-grazing heights under rotational stocking
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CARDOSO, Rebeca Rocha, primary, SOUSA, Luciano Fernandes de, additional, FERREIRA, Ana Cristina Holanda, additional, NEIVA, José Neuman Miranda, additional, FERREIRA, Deborah Alves, additional, and SILVA, André Guimarães Maciel e, additional
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- 2019
- Full Text
- View/download PDF
18. Intestinal helminth infection transforms the CD4+T cell composition of the skin
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Classon, Cajsa H., Li, Muzhen, Clavero, Ada Lerma, Ma, Junjie, Feng, Xiaogang, Tibbitt, Christopher A., Stark, Julian M., Cardoso, Rebeca, Ringqvist, Emma, Boon, Louis, Villablanca, Eduardo J., Rothfuchs, Antonio Gigliotti, Eidsmo, Liv, Coquet, Jonathan M., and Nylén, Susanne
- Abstract
Intestinal helminth parasites can alter immune responses to vaccines, other infections, allergens and autoantigens, implying effects on host immune responses in distal barrier tissues. We herein show that the skin of C57BL/6 mice infected with the strictly intestinal nematode Heligmosomoides polygyruscontain higher numbers of CD4+T cells compared to the skin of uninfected controls. Accumulated CD4+T cells were H. polygyrus-specific TH2 cells that skewed the skin CD4+T cell composition towards a higher TH2/TH1 ratio which persisted after worm expulsion. Accumulation of TH2 cells in the skin was associated with increased expression of the skin-homing chemokine receptors CCR4 and CCR10 on CD4+T cells in the blood and mesenteric lymph nodes draining the infected intestine and was abolished by FTY720 treatment during infection, indicating gut-to-skin trafficking of cells. Remarkably, skin TH2 accumulation was associated with impaired capacity to initiate IFN-γrecall responses and develop skin-resident memory cells to mycobacterial antigens, both during infection and months after deworming therapy. In conclusion, we show that infection by a strictly intestinal helminth has long-term effects on immune cell composition and local immune responses to unrelated antigens in the skin, revealing a novel process for T cell colonisation and worm-mediated immunosuppression in this organ.
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- 2022
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19. Avaliação de sorventes naturais para remediação de petróleo derramado em águas marinhas costeiras: o estado da arte e um estudo de caso aplicado
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Cardoso, Célia Karina Maia, Cardoso, Rebeca da Paixão Gomes, and Moreira, Ícaro Thiago Andrade
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Hidrocarbonetos ,Impactos ,Fibra de coco ,Petróleo ,Fibra capilar ,Remediação ,Sorventes ,Limpeza - Abstract
Submitted by Gisele Mara Hadlich (gisele@ufba.br) on 2019-01-08T17:28:16Z No. of bitstreams: 1 5006-20056-1-PB.pdf: 2058053 bytes, checksum: 09d17dffecd502007f1d9a8d9808ebc7 (MD5) Approved for entry into archive by Rafael Nunes (rafaeln@ufba.br) on 2019-02-21T17:12:13Z (GMT) No. of bitstreams: 1 5006-20056-1-PB.pdf: 2058053 bytes, checksum: 09d17dffecd502007f1d9a8d9808ebc7 (MD5) Made available in DSpace on 2019-02-21T17:12:13Z (GMT). No. of bitstreams: 1 5006-20056-1-PB.pdf: 2058053 bytes, checksum: 09d17dffecd502007f1d9a8d9808ebc7 (MD5) Previous issue date: 2017 A utilização do petróleo traz inúmeros benefícios à sociedade, porém sua produção, transporte e armazenamento podem ocasionar diversos impactos ao meio ambiente. Atualmente alguns métodos são aplicados na tentativa de solucionar ou amenizar os impactos gerados por este contaminante. Entretanto o uso destas técnicas pode ser nocivo ao meio ambiente. Este artigo traz como objetivo, sintetizar o uso de técnicas remediadoras ressaltando a utilização de sorventes através de um estado da arte. Destaca-se ainda, a avaliação e análise da fibra de coco e capilar como um artifício alternativo de limpeza das águas costeiras contaminadas pelo óleo. Seminário Estudantil de Produção Acadêmica
- Published
- 2017
20. Commensal Bacteria-Specific CD4+ T Cell Responses in Health and Disease
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Sorini, Chiara, primary, Cardoso, Rebeca F., additional, Gagliani, Nicola, additional, and Villablanca, Eduardo J., additional
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- 2018
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21. Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8(+) T Cells during Bacterial Infection
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Platteel, Anouk C M, Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H, Cardoso, Rebeca, Kloetzel, Peter M, Mishto, Michele, Sijts, Alice J A M, Platteel, Anouk C M, Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H, Cardoso, Rebeca, Kloetzel, Peter M, Mishto, Michele, and Sijts, Alice J A M
- Abstract
Proteasome-catalyzed peptide splicing (PCPS) generates peptides that are presented by MHC class I molecules, but because their identification is challenging, the immunological relevance of spliced peptides remains unclear. Here, we developed a reverse immunology-based multi-level approach to identify proteasome-generated spliced epitopes. Applying this strategy to a murine Listeria monocytogenes infection model, we identified two spliced epitopes within the secreted bacterial phospholipase PlcB that primed antigen-specific CD8(+) T cells in L. monocytogenes-infected mice. While reacting to the spliced epitopes, these CD8(+) T cells failed to recognize the non-spliced peptide parts in the context of their natural flanking sequences. Thus, we here show that PCPS expands the CD8(+) T cell response against L. monocytogenes by exposing spliced epitopes on the cell surface. Moreover, our multi-level strategy opens up opportunities to systematically investigate proteins for spliced epitope candidates and thus strategies for immunotherapies or vaccine design.
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- 2017
22. Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8(+) T Cells during Bacterial Infection
- Author
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dI&I RA-I&I I&I, Platteel, Anouk C M, Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H, Cardoso, Rebeca, Kloetzel, Peter M, Mishto, Michele, Sijts, Alice J A M, dI&I RA-I&I I&I, Platteel, Anouk C M, Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H, Cardoso, Rebeca, Kloetzel, Peter M, Mishto, Michele, and Sijts, Alice J A M
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- 2017
23. Development of pharmaceutical techniques to obtain the drug plant from the leaves of lemon balm (Lippia alba (Mill.) N.E Brown) chemotipo II
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Cardoso, Rebeca Sales and Bandeira, Mary Anne Medeiros
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Ciências Farmacêuticas ,Preparações Farmacêuticas ,Lippia - Abstract
Lippia alba, which belongs to the Verbenaceae family, is found in practically all regions of Brazil. Its popular names are lemon balm, false-melissa, with soothing and anxiolytic action. There are three chemotypes in the Northeast. Chemotype I, rich in the myrcene and citral (general and geranial) monoterpenes; Chemotype II (limonene and citral) and chemotype III (limonene and carvone). L. alba, chemootype II, was selected by the State Phytotherapy Committee for use in the Living Pharmacies of the State of Ceará, being part of REPLAME. It has the same active chemical marker (citral) as the chemootype I and the species Melissa officinalis and Cymbopogon citratus, and it is necessary to determine the chemical analytical marker to differentiate these species for quality control purposes, according to RDC nº 26/2014. The objective of this work was to conduct a pharmaceutical study of the leaves of L. alba, chemotype II, with viability of techniques for preparation of the plant drug with monitoring of the active marker (citral) and identification of the analytical marker. For this, a phytochemical approach was performed, evidencing the presence of steroids, free phenols, flavonoids, triterpenoids, saponins and condensed tannins, followed by the morphological and anatomical characterization of the leaves, presenting unicellular epidermis, paliçadic and lacunar parenchyma and oil secreting glandular trichomes Essential, with soft texture and citrus aroma. Subsequently, the preliminary tests were preceded, where the quality specifications were obtained, such as the determination of the loss by desiccation and the ash content. The chemical composition of the essential oil was evaluated by GC / MS during the period of May / 1988 to March / 2016, in which an increase in the oil content was observed, influenced by the number of cuts undergone, highlighting as major components, geranium and limonene . Then, the essential oil of the fresh and dried leaves, in the shade at room temperature, and in an oven at 40ºC, by coagulation and water vapor entrainment was analyzed by GC / MS. The 20% ethanolic extract and the essential oil were compared by CCD, evidencing spots with Rfs and similar behaviors. The 20% ethanolic extract was separated by CCA, isolated a substance by CEM and analyzed by 1H NMR, being called ECII of analytical chemical marker of L. alba species. The technique of steam trapping and desiccation of leaves at room temperature for 5 days proved to be the method of obtaining the essential oil with higher yield, more viable and lower cost. Lippia alba, pertecente à família Verbenaceae, é encontrada em praticamente todas as regiões do Brasil. Seus nomes populares são erva-cidreira, falsa-melissa, com ação calmante e ansiolítica. Existem no Nordeste três quimiotipos. Quimiotipo I, rico nos monoterpenos mirceno e citral (neral e geranial); quimiotipo II (limoneno e citral) e o quimiotipo III (limoneno e carvona). L. alba, quimiotipo II, foi selecionada pelo Comitê Estadual de Fitoterapia para uso nas Farmácias Vivas do Estado do Ceará, fazendo parte da REPLAME. Apresenta mesmo marcador químico ativo (citral) que o quimiotipo I e as espécies Melissa officinalis e Cymbopogon citratus, havendo a necessidade de determinar o marcador químico analítico para diferenciar essas espécies, com fins de controle de qualidade, conforme RDC nº 26/2014. O presente trabalho tem como objetivo realizar oestudo farmacêutico das folhas de L. alba, quimiotipo II, com viabilização de técnicas para preparação da droga vegetal com monitoramento do marcador ativo (citral) e a identificação do marcador analítico. Para isso, foi realizada, previamente uma abordagem fitoquímica, evidenciando a presença de esteróides, fenóis livres, flavonóides, triterpenóides, saponinas e taninos condensados, seguida da caracterização morfoanatômica das folhas, apresentando epidermes unicelulares, parênquima paliçádico e lacunoso e tricomas glandulares secretores de óleo essencial, com textura macia e aroma cítrico. Posteriormente, precedeu-se aos ensaios preliminares, onde foram obtidas as especificações de qualidade, como a determinação da perda por dessecação e do teor de cinzas. A composição química do óleo essencial foi avaliada por CG/EM durante o período de maio/1988 a março/2016 em que foi visto um aumento no teor do óleo, influenciado pelo número de cortes sofridos, destacando como componentes majoritários neral, geranial e limoneno. Em seguida, o óleo essencial das folhas frescas e dessecadas, à sombra em temperatura ambiente, e em estufa a 40ºC, por coobação e arraste à vapor d’água foi analisado por CG/EM. O extrato etanólico 20% e o óleo essencial foramcomparados por CCD, evidenciando manchas com Rfs e comportamentos semelhantes. O extrato etanólico 20% foi separado por CCA, isolada uma substância por CEM e analisada por RMN 1H, sendo denominada ECII de marcador químico analítico da espécie L. alba. A técnica de arraste à vapor d’água e a dessecação de folhas em temperatura ambiente por 5 dias, demonstrou ser o método de obtenção do óleo essencial com maior rendimento, mais viável e de menor custo.
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- 2016
24. Liver X receptor regulates Th17 and RORγt+Treg cells by distinct mechanisms
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Parigi, Sara M., Das, Srustidhar, Frede, Annika, Cardoso, Rebeca F., Tripathi, Kumar Parijat, Doñas, Cristian, Hu, Yue O. O., Antonson, Per, Engstrand, Lars, Gustafsson, Jan-Åke, and Villablanca, Eduardo J.
- Abstract
The gastrointestinal microenvironment, dominated by dietary compounds and the commensal bacteria, is a major driver of intestinal CD4+T helper (Th) cell differentiation. Dietary compounds can be sensed by nuclear receptors (NRs) that consequently exert pleiotropic effects including immune modulation. Here, we found that under homeostatic conditions the NR Liver X receptor (LXR), a sensor of cholesterol metabolites, regulates RORγt+CD4 T cells in the intestine draining mesenteric lymph node (MLN). While LXR activation led to a decrease, LXR-deficiency resulted in an increase in MLN Th17 and RORγt+Tregs. Mechanistically, LXR signaling in CD11c+myeloid cells was required to control RORγt+Treg. By contrast, modulation of MLN Th17 was independent of LXR signaling in either immune or epithelial cells. Of note, horizontal transfer of microbiota between LXRα−/−and WT mice was sufficient to only partially increase MLN Th17 in WT mice. Despite LXRα deficiency resulted in an increased abundance of Ruminococcaceae and Lachnospiraceae bacterial families compared to littermate controls, microbiota ablation (including SFB) was not sufficient to dampen LXRα-mediated expansion of MLN Th17. Altogether, our results suggest that LXR modulates RORγt+Treg and Th17 cells in the MLN through distinct mechanisms.
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- 2021
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25. Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8+ T Cells during Bacterial Infection
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Platteel, Anouk C.M., primary, Liepe, Juliane, additional, Textoris-Taube, Kathrin, additional, Keller, Christin, additional, Henklein, Petra, additional, Schalkwijk, Hanna H., additional, Cardoso, Rebeca, additional, Kloetzel, Peter M., additional, Mishto, Michele, additional, and Sijts, Alice J.A.M., additional
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- 2017
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26. Nutritional quality of massai grass fertilized with phosphorus and nitrogen and its influence on intake and weight gain of sheep under rotational grazing on quartzipsamment soil
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Silva, Thais Valéria Souza, primary, Sousa, Luciano Fernandes, additional, Santos, Antônio Clementino dos, additional, Ferreira, Ana Cristina Holanda, additional, Cardoso, Rebeca Rocha, additional, Sousa, Jhone Tallison Lira de, additional, Jardim, Wanderson Campos, additional, Ricardo, Anderson Da Silva, additional, Almeida, Josimar Santos de, additional, and Carvalho, Junior Bezerra de, additional
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- 2017
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27. Performance of Seeds Crambe Exposed to Pre-germination Treatments
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Nobre, Danúbia Aparecida Costa, Cardoso, Rebeca Rocha, David, Andréia Márcia Santos de Souza, Amaro, Hugo Tiago Ribeiro, Borghetti, Renato Antônio, and Costa, Márcia Regina
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oilseed ,dormancy ,velocidad de germinación ,QH301-705.5 ,dormência ,oleaginosa ,germination speed ,Biology (General) ,dormancia ,velocidade de germinação ,semillas oleaginosas - Abstract
Com o estímulo à produção e uso de biodiesel, sementes de crambe constituem hoje uma das melhores opções para o fornecimento de matéria-prima, sendo também excelente alternativa para cultivo de outono-inverno visando rotação de culturas. O objeto deste estudo foi avaliar o efeito da combinação de tratamentos de pré-germinação sobre o comportamento de sementes de crambe. A partir de um lote de sementes da cultivar FMS Brilhante, foi proposto o delineamento experimental inteiramente casualizado, em esquema fatorial 2 x 5. O delineamento foi baseado na combinação de duas condições estruturais: sementes com e sem pericarpo, e ambas submetidas a tratamentos pré-germinativos com e sem ácido giberélico, sendo: testemunha (sem tratamento); sementes pré-embebidas em água destilada sem adição de giberelina por 24 horas como controle; e sementes pré- embebidas em solução de ácido giberélico a 4 %, em diferentes concentrações (400; 500 e 600 mg.L -1 ). Determinou-se o teor de água, primeira contagem de germinação, porcentagem de germinação, emergência de plântulas e índice de velocidade de emergência. Diante dos resultados conclui-se que a retirada do pericarpo em sementes de crambe, cultivar FMS Brilhante, acelera a velocidade de germinação, porém, diminui a sua porcentagem final. A pré-embebição em ácido giberélico (400, 500 e 600 mg.L -1 ) por 24 horas proporcionou incrementos na germinação e no vigor das sementes de crambe com pericarpo. Encouraging the production and use of biodiesel, seeds of crambe today constitute one of the best options for the supply of raw material, is also an excellent alternative for autumn-winter crop rotation order. The aim of this study was to evaluate the efficiency of combined pre-germination treatments on the seed behavior of crambe. From a seed sample of FMS Brilhante cultivar, an experimental design completely randomized with a 2 x 5 factorial arrangement was performed. It was formed from combination of two structural conditions, seeds with or without pericarp, and treatments with or without giberelic acid, being: control (no treatment); seeds pre-soaked in distilled water for 24 hours as control; and seeds pre-soaked in gibberellic acid at 4 % at different concentrations (400, 500 and 600 mg.L -1 ). Water content, first count germination, germination, seedling emergence and emergence rate index were determined. From these results it is concluded that removal of the pericarp in seed of crambe, cultivar FMS Brilhante, accelerated the germination rate, however, decrea- sed your final percentage. The pre-soaking in gibberellic acid (400, 500 and 600 mg L -1 ) for 24 hours, increased the germination and seed vigor crambe with pericarp. Como un estímulo a la producción y uso del biodiesel, las semillas de crambe constituyen hoy una de las mejores op- ciones para el suministro de materia prima, también son una excelente alternativa en la rotación de cultivos de otoño- invierno. El objetivo de este estudio fue evaluar la eficiencia de la combinación de tratamientos pre-germinativos sobre el comportamiento de semillas de crambe. A partir de una muestra de semillas del cultivar FMS Brilhante, se propuso un diseño experimental completamente aleatorizado, con un esquema factorial 2 x 5. El diseño se basó en la combinación de dos condiciones estructurales: semillas con y sin pericar- pio, y ambos sometidos a tratamentos pre-germinativos con y sin ácido giberélico, siendo: control (sin tratamiento); se- millas pre-embebidas en agua destilada sin giberelina por 24 horas como control; y semillas pre-embebidas en solución de ácido giberélico a 4 %, en diferentes concentraciones (400; 500 e 600 mg.L -1 ). Se determinó el contenido de agua, primer contaje de germinación y porcentaje de germinación, emergen- cia de plántulas e índice de velocidad de emergencia. A partir de estos resultados se concluye que la remoción del pericarpio en semillas de crambe, cultivar FMS Brilhante, aceleró la velo- cidad de germinación, pero, disminuyó su porcentaje final. La pre-imbibición en ácido giberélico (400, 500 e 600 mg.L -1 ) por 24 horas proporcionó incrementos en la germinación y en el vigor de las semillas de crambe con pericarpio.
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- 2014
28. Desempeño de semillas de crambe sometidas a tratamientos pre-germinativos
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Cardoso, Rebeca Rocha, Costa Nobre, Danúbia Aparecida, Santos de Souza David, Andréia Márcia, Ribeiro Amaro, Hugo Tiago, Borghetti, Renato Antônio, and Costa, Márcia Regina
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oilseed ,dormancy ,dormência ,Crambe abyssinica Hochst ,Agronomia ,Ciencias agrárias ,velocidad de germinación ,germination speed.dormancia ,velocidade de germinação ,semillas oleaginosas - Abstract
El objetivo del trabajo fue evaluar la eficiencia de los tratamientos pre-germinativos en el desempeño de las semillas de crambe (Crambe abyssinica Hochst.). El diseño experimental fue completamente al azar, con arreglo factorial 2 x 5, los tratamientos están constituidos por la combinación de dos lotes de semillas y cinco tratamientos pre-germinativos, con cuatro repeticiones de 50 semillas. Los lotes fueron formados por dos condiciones físicas: con y sin pericarpio. Los tratamientos empleados fueron: control (sin tratamiento); semillas pre-embebidas en solución de ácido giberélico a 4%, por 24 horas en diferentes concentraciones (400, 500 y 600 mg.L-1), y semillas pre-embebidas en agua destilada por 24 h como control (0,0). Se determinó el contenido de agua, porcentaje de germinación, primer contaje de germinación, emergencia de plántulas e índice de velocidad de emergencia. La remoción del pericarpio en semillas de crambe acelera la velocidad de germinación, pero, disminuye su porcentaje final. La pre-imbibición en ácido giberélico (400, 500 e 600 mg.L-1) por 24 horas proporcionó incrementos en la germinación y en el vigor de las semillas de crambe con pericarpio. Encouraging the production and use of biodiesel, seeds of crambe today constitute one of the best options for the supply of raw material, is also an excellent alternative for autumn-winter crop rotation order. The aim of this study was to evaluate the efficiency of combined pre-germination treatments on the seed behavior of crambe. From a seed sample of FMS Brilhante cultivar, an experimental design completely randomized with a 2 x 5 factorial arrangement was performed. It was formed from combination of two structural conditions, seeds with or without pericarp, and treatments with or without giberelic acid, being: control (no treatment); seeds pre-soaked in distilled water for 24 hours as control; and seeds pre-soaked in gibberellic acid at 4 % at different concentrations (400, 500 and 600 mg.L-1). Water content, first count germination, germination, seedling emergence and emergence rate index were determined. From these results it is concluded that removal of the pericarp in seed of crambe, cultivar FMS Brilhante, accelerated the germination rate, however, decreased your final percentage. The pre-soaking in gibberellic acid (400, 500 and 600 mg L-1) for 24 hours, increased the germination and seed vigor crambe with pericarp.DESEMPENHO DE SEMENTES DE CRAMBE EXPOSTAS À TRATAMENTOS PRÉ-GERMINATIVOSCom o estímulo à produção e uso de biodiesel, sementes de crambe constituem hoje uma das melhores opções para o fornecimento de matéria-prima, sendo também excelente alternativa para cultivo de outono-inverno visando rotação de culturas. O objeto deste estudo foi avaliar o efeito da combinação de tratamentos de pré-germinação sobre o comportamento de sementes de crambe. A partir de um lote de sementes da cultivar FMS Brilhante, foi proposto o delineamento experimental inteiramente casualizado, em esquema fatorial 2 x 5. O delineamento foi baseado na combinação de duas condições estruturais: sementes com e sem pericarpo, e ambas submetidas a tratamentos pré-germinativos com e sem ácido giberélico, sendo: testemunha (sem tratamento); sementes pré-embebidas em água destilada sem adição de giberelina por 24 horas como controle; e sementes pré-embebidas em solução de ácido giberélico a 4 %, em diferentes concentrações (400; 500 e 600 mg.L-1). Determinou-se o teor de água, primeira contagem de germinação, porcentagem de germinação, emergência de plântulas e índice de velocidade de emergência. Diante dos resultados conclui-se que a retirada do pericarpo em sementes de crambe, cultivar FMS Brilhante, acelera a velocidade de germinação, porém, diminui a sua porcentagem final. A pré-embebição em ácido giberélico (400, 500 e 600 mg.L-1) por 24 horas proporcionou incrementos na germinação e no vigor das sementes de crambe com pericarpo. Comportamiento de semillas de crambe sometidas a tratamientos pre-germinativos Como un estímulo a la producción y uso del biodiesel las semillas de crambe constituyen hoy una de las mejores opciones para el suministro de materia prima, también son una excelente alternativa en la rotación de cultivos de otoño-invierno. El objetivo de este estudio fue evaluar la eficiencia de la combinación de tratamientos pre-germinativos sobre el comportamiento de semillas de crambe. A partir de una muestra de semillas del cultivar FMS Brilhante, se propuso un diseño experimental completamente aleatorizado, con un esquema factorial 2 x 5. El diseño se basó en la combinación de dos condiciones estructurales: semillas con y sin pericarpio, y ambos sometidos a tratamentos pré-germinativos con y sin ácido giberélico, siendo: control (sin tratamiento); semillas pre-embebidas en agua destilada sin giberelina por 24 horas como control; y semillas pré-embebidas en solución de ácido giberélico a 4 %, en diferentes concentraciones (400; 500 e 600 mg.L-1). Se determinó el contenido de agua, primer contaje de germinación y porcentaje de germinación, emergencia de plántulas e índice de velocidad de emergencia. A partir de estos resultados se concluye que la remoción del pericarpio en semillas de crambe, cultivar FMS Brilhante, aceleró la velocidad de germinación, pero, disminuyó su porcentaje final. La pre-imbibición en ácido giberélico (400, 500 e 600 mg.L-1) por 24 horas proporcionó incrementos en la germinación y en el vigor de las semillas de crambe con pericarpio.
- Published
- 2014
29. Caracterização morfológica e agronômica de variedades de arroz-vermelho (Oryza sativa L.)
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CARDOSO, Rebeca Rocha, MATOS, Valderez Pontes, CARVALHO FILHO, José Luiz Sandes de, VIANA, Jeandson Silva, and ANUNCIAÇÃO FILHO, Clodoaldo José da
- Subjects
Red rice ,MELHORAMENTO VEGETAL [FITOTECNIA] ,Descritor agronômico ,Morphological descriptor ,Oryza sativa ,Genetic variability ,Variabilidade genética ,Agronomic descriptor ,Arroz-vermelho ,Descritor morfológico - Abstract
Submitted by Mario BC (mario@bc.ufrpe.br) on 2016-09-20T11:40:43Z No. of bitstreams: 1 Rebeca Rocha Cardoso.pdf: 3507705 bytes, checksum: 7b8fdfe5d7395b5f99a73f29bccf1d05 (MD5) Made available in DSpace on 2016-09-20T11:40:43Z (GMT). No. of bitstreams: 1 Rebeca Rocha Cardoso.pdf: 3507705 bytes, checksum: 7b8fdfe5d7395b5f99a73f29bccf1d05 (MD5) Previous issue date: 2013-07-26 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Red rice belongs to the same species of traditional rice (Oryza sativa L.) and was introduced in Brazil in the seventeenth century, when it became widely consumed in the country. In Brazil, the central zone of semi-arid northeastern region stands out as producer and consumer. This particular rice, rich in proteins, minerals and vitamins B1, differs from the traditional white rice, among other things, the kernel color, shape and composition, as well as longer cycle, pubescence and low productivity, the latter being aggravated by low selection due to limited cultivation. Morphological characterization and agronomic varieties of red rice and used in existing germplasm collections and seed banks is an important tool to be used in future breeding programs. Thus, as the goal of breeding is to rescue, preserve and generate genetic variability for crop areas is important to have knowledge of the characters of cultivated varieties from morphological and agronomic. Based on this importance, the present study was to characterize six varieties of red rice (Vermelho, Cáqui, MNA PB 0405, MNA PB 0728, MNA RN 0802 e MNA RN 0803) through morphological and agronomic, to obtain information to assist in the selection of rice varieties Red-promising and better use of available germplasm. There was green leaf color, pubescence average limbo aurícolas of pale green, ligules colorless, erect flag leaf, straw-yellow color in the glumes, lemma and palea hairy, absence of awns and easy shattering, tall and panicle averages in all varieties, straw color of palea and lemma and apiculus grain, caryopsis red and half-elongated grains in most varieties. The varieties MNA PB 0728, MNA RN 0802 and Cáqui belong to the class of long grain, MNA PB 0405 to medium grain, while the varieties Vermelho and MNA RN 0803 to the class of mixed grains. The seeds of all varieties have translucent and chalky endosperm. The varieties MNA PB 0405, MNA RN 0802 and MNA RN 0803 were the varieties with high germination percentage. Only varietie MNA RN 0803 show germination above the minimum limits allowed. O arroz-vermelho pertence à mesma espécie do arroz tradicional (Oryza sativa L.) e foi introduzido no Brasil no século XVII, quando passou a ser bastante consumido no país. No Brasil, a zona central do semiárido nordestino se destaca como região produtora e consumidora. Este arroz especial, rico em proteínas, sais minerais e vitaminas B1, se diferencia do arroz branco tradicional, entre outros aspectos, pela coloração dos grãos, forma e composição, além do ciclo mais longo, pubescência e baixa produtividade, agravada pela baixa seleção devido ao cultivo limitado. A caracterização morfológica e agronômica de variedades de arroz-vermelho utilizadas e existentes em coleções de germoplasma e bancos de sementes é uma ferramenta importante para ser usada em futuros programas de melhoramento genético. Assim, como o objetivo do melhoramento genético é resgatar, preservar e gerar variabilidade genética para as áreas de cultivo é importante que se tenha conhecimento dos caracteres das variedades cultivadas, a partir de caracterização morfológica e agronômica. Com base nesta importância, o presente trabalho foi realizado visando caracterizar seis variedades de arroz-vermelho (Vermelho, Cáqui, MNA PB 0405, MNA PB 0728, MNA RN 0802 e MNA RN 0803) através de descritores morfológicos e agronômicos e qualidade fisiológica de sementes, visando obter informações que auxiliem na seleção de variedades de arroz-vermelho promissoras e na melhor utilização do germoplasma disponível. Verificou-se cor da folha verde, pubescência média do limbo, aurícolas de cor verde-clara, lígulas incolores, folha bandeira de postura ereta, cor amarelo-palha nas glumelas, lema e pálea pilosa, ausência de aristas e fácil degrane, porte alto e panículas médias em todas as variedades, cor palha da lema e pálea e do apículo do grão, cor vermelha da cariopse e grãos meio-alongados na maioria das variedades. As variedades MNA PB 0728, MNA RN 0802 e Cáqui pertencem à classe de grãos longos, MNA PB 0405 a de grãos médios, enquanto as variedades Vermelho e MNA RN 0803 à classe de grãos misturados. As sementes de todas as variedades estudadas possuem endosperma translúcido e farináceo. As variedades MNA PB 0405, MNA RN 0802 e MNA 0803 foram as variedades com maiores porcentagens de germinação. Apenas a variedade MNA RN 0803 apresentou germinação acima dos limites mínimos permitidos.
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- 2013
30. Commensal Bacteria-Specific CD4+ T Cell Responses in Health and Disease.
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Sorini, Chiara, Cardoso, Rebeca F., Gagliani, Nicola, and Villablanca, Eduardo J.
- Abstract
Over the course of evolution, mammalian body surfaces have adapted their complex immune system to allow a harmless coexistence with the commensal microbiota. The adaptive immune response, in particular CD4
+ T cell-mediated, is crucial to maintain intestinal immune homeostasis by discriminating between harmless (e.g., dietary compounds and intestinal microbes) and harmful stimuli (e.g., pathogens). To tolerate food molecules and microbial components, CD4+ T cells establish a finely tuned crosstalk with the environment whereas breakdown of these mechanisms might lead to chronic disease associated with mucosal barriers and beyond. How commensal-specific immune responses are regulated and how these molecular and cellular mechanisms can be manipulated to treat chronic disorders is yet poorly understood. In this review, we discuss current knowledge of the regulation of commensal bacteria-specific CD4+ T cells. We place particular focus on the key role of commensal-specific CD4+ T cells in maintaining tolerance while efficiently eradicating local and systemic infections, with a focus on factors that trigger their aberrant activation. [ABSTRACT FROM AUTHOR]- Published
- 2018
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- View/download PDF
31. Corrigendum to: Characterization of the transport signals that mediate the nucleocytoplasmic traffic of low risk HPV11 E7 [Virology 443 (2013) 113–122]
- Author
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McKee, Courtney H., primary, Onder, Zeynep, additional, Ashok, Aditya, additional, Cardoso, Rebeca, additional, and Moroianu, Junona, additional
- Published
- 2015
- Full Text
- View/download PDF
32. Performance of Seeds Crambe Exposed to Pre-germination Treatments
- Author
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Cardoso, Rebeca Rocha, primary, Costa Nobre, Danúbia Aparecida, additional, Santos de Souza David, Andréia Márcia, additional, Ribeiro Amaro, Hugo Tiago, additional, Borghetti, Renato Antônio, additional, and Costa, Márcia Regina, additional
- Published
- 2014
- Full Text
- View/download PDF
33. Characterization of the transport signals that mediate the nucleocytoplasmic traffic of low risk HPV11 E7
- Author
-
McKee, Courtney H., primary, Onder, Zeynep, additional, Ashok, Aditya, additional, Cardoso, Rebeca, additional, and Moroianu, Junona, additional
- Published
- 2013
- Full Text
- View/download PDF
34. Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8+T Cells during Bacterial Infection
- Author
-
Platteel, Anouk C.M., Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H., Cardoso, Rebeca, Kloetzel, Peter M., Mishto, Michele, and Sijts, Alice J.A.M.
- Abstract
Proteasome-catalyzed peptide splicing (PCPS) generates peptides that are presented by MHC class I molecules, but because their identification is challenging, the immunological relevance of spliced peptides remains unclear. Here, we developed a reverse immunology-based multi-level approach to identify proteasome-generated spliced epitopes. Applying this strategy to a murine Listeria monocytogenesinfection model, we identified two spliced epitopes within the secreted bacterial phospholipase PlcB that primed antigen-specific CD8+T cells in L. monocytogenes-infected mice. While reacting to the spliced epitopes, these CD8+T cells failed to recognize the non-spliced peptide parts in the context of their natural flanking sequences. Thus, we here show that PCPS expands the CD8+T cell response against L. monocytogenesby exposing spliced epitopes on the cell surface. Moreover, our multi-level strategy opens up opportunities to systematically investigate proteins for spliced epitope candidates and thus strategies for immunotherapies or vaccine design.
- Published
- 2017
- Full Text
- View/download PDF
35. DESEMPENHO DE SEMENTES DE CRAMBE EXPOSTAS À TRATAMENTOS PRÉ-GERMINATIVOS.
- Author
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ROCHA CARDOSO, REBECA, COSTA NOBRE, DANÚBIA APARECIDA, SANTOS DE SOUZA DAVID, ANDRÉIA MÁRCIA, RIBEIRO AMARO, HUGO TIAGO, BORGHETTI, RENATO ANTÔNIO, and COSTA, MÁRCIA REGINA
- Subjects
- *
OILSEEDS , *GERMINATION , *DORMANCY in plants , *CROP rotation research , *CULTIVARS - Abstract
Encouraging the production and use of biodiesel, seeds of crambe today constitute one of the best options for the supply of raw material, is also an excellent alternative for autumn-winter crop rotation order. The aim of this study was to evaluate the efficiency of combined pre-germination treatments on the seed behavior of crambe. From a seed sample of FMS Brilhante cultivar, an experimental design completely randomized with a 2 x 5 factorial arrangement was performed. It was formed from combination of two structural conditions, seeds with or without pericarp, and treatments with or without giberelic acid, being: control (no treatment); seeds pre-soaked in distilled water for 24 hours as control; and seeds pre-soaked in gibberellic acid at 4 % at different concentrations (400, 500 and 600 mg.L-1). Water content, first count germination, germination, seedling emergence and emergence rate index were determined. From these results it is concluded that removal of the pericarp in seed of crambe, cultivar FMS Brilhante, accelerated the germination rate, however, decreased your final percentage. The pre-soaking in gibberellic acid (400, 500 and 600 mg L-1) for 24 hours, increased the germination and seed vigor crambe with pericarp. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
36. Commensal Bacteria-Specific CD4 + T Cell Responses in Health and Disease.
- Author
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Sorini C, Cardoso RF, Gagliani N, and Villablanca EJ
- Subjects
- Animals, Humans, Adaptive Immunity, Bacteria immunology, CD4-Positive T-Lymphocytes immunology, Gastrointestinal Microbiome immunology, Immune Tolerance
- Abstract
Over the course of evolution, mammalian body surfaces have adapted their complex immune system to allow a harmless coexistence with the commensal microbiota. The adaptive immune response, in particular CD4
+ T cell-mediated, is crucial to maintain intestinal immune homeostasis by discriminating between harmless (e.g., dietary compounds and intestinal microbes) and harmful stimuli (e.g., pathogens). To tolerate food molecules and microbial components, CD4+ T cells establish a finely tuned crosstalk with the environment whereas breakdown of these mechanisms might lead to chronic disease associated with mucosal barriers and beyond. How commensal-specific immune responses are regulated and how these molecular and cellular mechanisms can be manipulated to treat chronic disorders is yet poorly understood. In this review, we discuss current knowledge of the regulation of commensal bacteria-specific CD4+ T cells. We place particular focus on the key role of commensal-specific CD4+ T cells in maintaining tolerance while efficiently eradicating local and systemic infections, with a focus on factors that trigger their aberrant activation.- Published
- 2018
- Full Text
- View/download PDF
37. Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8 + T Cells during Bacterial Infection.
- Author
-
Platteel ACM, Liepe J, Textoris-Taube K, Keller C, Henklein P, Schalkwijk HH, Cardoso R, Kloetzel PM, Mishto M, and Sijts AJAM
- Subjects
- Animals, CD8-Positive T-Lymphocytes pathology, Epitopes, T-Lymphocyte genetics, Listeriosis genetics, Listeriosis pathology, Mice, Proteasome Endopeptidase Complex genetics, CD8-Positive T-Lymphocytes immunology, Epitopes, T-Lymphocyte immunology, Listeria monocytogenes immunology, Listeriosis immunology, Proteasome Endopeptidase Complex immunology
- Abstract
Proteasome-catalyzed peptide splicing (PCPS) generates peptides that are presented by MHC class I molecules, but because their identification is challenging, the immunological relevance of spliced peptides remains unclear. Here, we developed a reverse immunology-based multi-level approach to identify proteasome-generated spliced epitopes. Applying this strategy to a murine Listeria monocytogenes infection model, we identified two spliced epitopes within the secreted bacterial phospholipase PlcB that primed antigen-specific CD8
+ T cells in L. monocytogenes-infected mice. While reacting to the spliced epitopes, these CD8+ T cells failed to recognize the non-spliced peptide parts in the context of their natural flanking sequences. Thus, we here show that PCPS expands the CD8+ T cell response against L. monocytogenes by exposing spliced epitopes on the cell surface. Moreover, our multi-level strategy opens up opportunities to systematically investigate proteins for spliced epitope candidates and thus strategies for immunotherapies or vaccine design., (Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.)- Published
- 2017
- Full Text
- View/download PDF
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