128,773 results on '"Carcinogenesis"'
Search Results
2. Improvements in Thyroid Tumor Surgery and the Prognosis, Diagnosis, Recurrence and Metastasis of Patients
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- 2024
3. Changes Associated With H. Pylori and Gastric Carcinogenesis (IIT H pylori)
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- 2024
4. Role of Sodium-glucose Linked Transporter 2 (SGLT2) and Its Inhibitor Over CARdiotoxicity Induced by Anthracyclines and Breast Cancer Tumorigenesis SCARA-B (SCARA-B)
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- 2024
5. An Integrative Multi-Omic Characterization of Head and Neck Carcinogenesis, Progression and Recurrence
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National Cancer Institute (NCI)
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- 2024
6. Evaluating Obesity-Mediated Mechanisms of Pancreatic Carcinogenesis in Minority Populations
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United States Department of Defense
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- 2024
7. AF1q is a universal marker of neuroblastoma that sustains N-Myc expression and drives tumorigenesis.
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Lee, Joanna, Asgharzadeh, Shahab, Khan, Ranjha, Zhang, Meng, Weisbrod, Julia, Choi, Youn-Jeong, Puri, Latika, Aguilar, Ana, Zhao, Piming, Saba, Julie, and Oskouian, Babak
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Child ,Humans ,N-Myc Proto-Oncogene Protein ,Neuroblastoma ,Oncogene Proteins ,Cell Transformation ,Neoplastic ,Transcription Factors ,Carcinogenesis ,Cell Line ,Tumor ,Gene Expression Regulation ,Neoplastic - Abstract
Neuroblastoma is the most common extracranial malignant tumor of childhood, accounting for 15% of all pediatric cancer deaths. Despite significant advances in our understanding of neuroblastoma biology, five-year survival rates for high-risk disease remain less than 50%, highlighting the importance of identifying novel therapeutic targets to combat the disease. MYCN amplification is the most frequent and predictive molecular aberration correlating with poor outcome in neuroblastoma. N-Myc is a short-lived protein primarily due to its rapid proteasomal degradation, a potentially exploitable vulnerability in neuroblastoma. AF1q is an oncoprotein with established roles in leukemia and solid tumor progression. It is normally expressed in brain and sympathetic neurons and has been postulated to play a part in neural differentiation. However, no role for AF1q in tumors of neural origin has been reported. In this study, we found AF1q to be a universal marker of neuroblastoma tumors. Silencing AF1q in neuroblastoma cells caused proteasomal degradation of N-Myc through Ras/ERK and AKT/GSK3β pathways, activated p53 and blocked cell cycle progression, culminating in cell death via the intrinsic apoptotic pathway. Moreover, silencing AF1q attenuated neuroblastoma tumorigenicity in vivo signifying AF1qs importance in neuroblastoma oncogenesis. Our findings reveal AF1q to be a novel regulator of N-Myc and potential therapeutic target in neuroblastoma.
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- 2024
8. Targeted inhibition of SCFSKP2 confers anti-tumor activities resulting in a survival benefit in osteosarcoma.
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Wang, Jichuan, Ferrena, Alexander, Zhang, Ranxin, Singh, Swapnil, Viscarret, Valentina, Al-Harden, Waleed, Aldahamsheh, Osama, Borjihan, Hasibagan, Singla, Amit, Yaguare, Simon, Tingling, Janet, Lo, Yungtai, Gorlick, Richard, Schwartz, Edward, Zhao, Hongling, Yang, Rui, Geller, David, Zheng, Deyou, Hoang, Bang, and Zi, Xiaolin
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Animals ,Humans ,Mice ,Bone Neoplasms ,Carcinogenesis ,Cyclin-Dependent Kinase Inhibitor p27 ,Mice ,Knockout ,Osteosarcoma ,S-Phase Kinase-Associated Proteins ,Tumor Microenvironment - Abstract
Osteosarcoma(OS) is a highly aggressive bone cancer for which treatment has remained essentially unchanged for decades. Although OS is characterized by extensive genomic heterogeneity and instability, RB1 and TP53 have been shown to be the most commonly inactivated tumor suppressors in OS. We previously generated a mouse model with a double knockout (DKO) of Rb1 and Trp53 within cells of the osteoblastic lineage, which largely recapitulates human OS with nearly complete penetrance. SKP2 is a repression target of pRb and serves as a substrate recruiting subunit of the SCFSKP2 complex. In addition, SKP2 plays a central role in regulating the cell cycle by ubiquitinating and promoting the degradation of p27. We previously reported the DKOAA transgenic model, which harbored a knock-in mutation in p27 that impaired its binding to SKP2. Here, we generated a novel p53-Rb1-SKP2 triple-knockout model (TKO) to examine SKP2 function and its potential as a therapeutic target in OS. First, we observed that OS tumorigenesis was significantly delayed in TKO mice and their overall survival was markedly improved. In addition, the loss of SKP2 also promoted an apoptotic microenvironment and reduced the stemness of DKO tumors. Furthermore, we found that small-molecule inhibitors of SKP2 exhibited anti-tumor activities in vivo and in OS organoids as well as synergistic effects when combined with a standard chemotherapeutic agent. Taken together, our results suggest that SKP2 inhibitors may reduce the stemness plasticity of OS and should be leveraged as next-generation adjuvants in this cancer.
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- 2024
9. Nuclear to cytoplasmic transport is a druggable dependency in MYC-driven hepatocellular carcinoma.
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Deutzmann, Anja, Sullivan, Delaney, Dhanasekaran, Renumathy, Li, Wei, Chen, Xinyu, Tong, Ling, Mahauad-Fernandez, Wadie, Bell, John, Mosley, Adriane, Koehler, Angela, Li, Yulin, and Felsher, Dean
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Humans ,Mice ,Animals ,Carcinoma ,Hepatocellular ,Liver Neoplasms ,Proto-Oncogene Proteins c-myc ,Genes ,myc ,Cell Transformation ,Neoplastic ,Carcinogenesis ,Cell Line ,Tumor ,Gene Expression Regulation ,Neoplastic - Abstract
The MYC oncogene is often dysregulated in human cancer, including hepatocellular carcinoma (HCC). MYC is considered undruggable to date. Here, we comprehensively identify genes essential for survival of MYChigh but not MYClow cells by a CRISPR/Cas9 genome-wide screen in a MYC-conditional HCC model. Our screen uncovers novel MYC synthetic lethal (MYC-SL) interactions and identifies most MYC-SL genes described previously. In particular, the screen reveals nucleocytoplasmic transport to be a MYC-SL interaction. We show that the majority of MYC-SL nucleocytoplasmic transport genes are upregulated in MYChigh murine HCC and are associated with poor survival in HCC patients. Inhibiting Exportin-1 (XPO1) in vivo induces marked tumor regression in an autochthonous MYC-transgenic HCC model and inhibits tumor growth in HCC patient-derived xenografts. XPO1 expression is associated with poor prognosis only in HCC patients with high MYC activity. We infer that MYC may generally regulate and require altered expression of nucleocytoplasmic transport genes for tumorigenesis.
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- 2024
10. Overexpression of TBX3 suppresses tumorigenesis in experimental and human cholangiocarcinoma
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Deng, Shanshan, Lu, Xinjun, Wang, Xue, Liang, Binyong, Xu, Hongwei, Yang, Doris, Cui, Guofei, Yonemura, Andrew, Paine, Honor, Zhou, Yi, Zhang, Yi, Simile, Maria Maddalena, Urigo, Francesco, Evert, Matthias, Calvisi, Diego F, Green, Benjamin L, and Chen, Xin
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Biological Sciences ,Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Cancer ,Digestive Diseases ,Rare Diseases ,Human Genome ,Genetics ,Liver Disease ,Liver Cancer ,Digestive Diseases - (Gallbladder) ,2.1 Biological and endogenous factors ,Aetiology ,Cholangiocarcinoma ,T-Box Domain Proteins ,Humans ,Animals ,Mice ,Bile Duct Neoplasms ,Carcinogenesis ,Cell Line ,Tumor ,Gene Expression Regulation ,Neoplastic ,Cell Proliferation ,Biochemistry and Cell Biology ,Biochemistry and cell biology ,Oncology and carcinogenesis - Abstract
TBX3 behaves as a tumor suppressor or oncoprotein across cancer. However, TBX3 function remains undetermined in intrahepatic cholangiocarcinoma (iCCA), a deadly primary liver malignancy with few systemic treatment options. This study sought to investigate the impact of TBX3 on iCCA. We found that overexpression of TBX3 strongly inhibited human iCCA cell growth. In the Akt/FBXW7ΔF mouse iCCA model, overexpression of Tbx3 reduced cholangiocarcinogenesis in vivo, while inducible genetic knockout of Tbx3 accelerated iCCA growth. RNA-seq identified MAD2L1 as a downregulated gene in TBX3-overexpressing cells, and ChIP confirmed that TBX3 binds to the MAD2L1 promoter. CRISPR-mediated knockdown of Mad2l1 significantly reduced the growth of two iCCA models in vivo. Finally, we found that TBX3 expression is upregulated in ~20% of human iCCA samples, and its high expression is associated with less proliferation and better survival. MAD2L1 expression is upregulated in most human iCCA samples and negatively correlated with TBX3 expression. Altogether, our findings suggest that overexpression of TBX3 suppresses CCA progression via repressing MAD2L1 expression.
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- 2024
11. Switching to Potential Reduced Exposure Products in Adult Smokers (ZYN)
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- 2024
12. Chemoprevention of Gastric Carcinogenesis
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National Cancer Institute (NCI), Cancer Prevention Pharmaceuticals, Inc., and Douglas Morgan, Director, Latin America sites, Vanderbilt Institute for Global Health
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- 2024
13. HPV Immunological Markers of Cervical Persistent Infection and Oncogenesis (HPVImmuno)
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Daniele Lilleri, Principal Investigator
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- 2024
14. Inhibition of Oral Tumorigenesis by Antitumor B
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Stuart Wong, Professor
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- 2024
15. Study on the Probiotics Regulating miRNA in H. Pylori-induced Wnt/β-catenin Gastric Carcinogenesis.
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Yao-Jong Yang, professor
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- 2024
16. Abnormal Food Timing and Circadian Dyssynchrony in Alcohol Induced Colon Carcinogenesis (AFT)
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Faraz Bishehsari, MD, PhD, Assistan Professor
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- 2024
17. Microplastics: an often-overlooked issue in the transition from chronic inflammation to cancer.
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Cheng, Yicong, Yang, Yang, Bai, Ling, and Cui, Jiuwei
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CARCINOGENESIS , *TUMOR microenvironment , *MICROPLASTICS , *INFLAMMATION , *CANCER invasiveness - Abstract
The presence of microplastics within the human body has raised significant concerns about their potential health implications. Numerous studies have supported the hypothesis that the accumulation of microplastics can trigger inflammatory responses, disrupt the microbiome, and provoke immune reactions due to their physicochemical properties. Chronic inflammation, characterized by tissue damage, angiogenesis, and fibrosis, plays a crucial role in cancer development. It influences cancer progression by altering the tumor microenvironment and impairing immune surveillance, thus promoting tumorigenesis and metastasis. This review explores the fundamental properties and bioaccumulation of microplastics, as well as their potential role in the transition from chronic inflammation to carcinogenesis. Additionally, it provides a comprehensive overview of the associated alterations in signaling pathways, microbiota disturbances, and immune responses. Despite this, the current understanding of the toxicity and biological impacts of microplastics remains limited. To mitigate their harmful effects on human health, there is an urgent need to improve the detection and removal methods for microplastics, necessitating further research and elucidation. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Environment and gynaecologic cancers.
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Chandra, Rudrika and Kumari, Sarita
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GYNECOLOGIC cancer , *BREAST cancer , *LUNG cancer , *CARCINOGENESIS , *INFECTION - Abstract
In the current era, environmental factors are well established as major causative agents for all cancers especially lung and breast cancer. We sought to review the current available literature on the topic pertaining to gynaecologic cancers. Although a few factors are well established in literature, others need more research to conclude. [ABSTRACT FROM AUTHOR]
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- 2024
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19. Understanding bladder cancer risk: Mendelian randomization analysis of immune cell and inflammatory factor influence.
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Hiocheng Un, Wumier Wusimanjiang, Wenhao Zhan, Xinxin Zhang, Minghao Li, Jiahao Lei, Renxuan Lin, Yuliang Zhang, Junxing Chen, and Zongren Wang
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GENOME-wide association studies ,DISEASE risk factors ,T cells ,CARCINOGENESIS ,INVERSE relationships (Mathematics) ,BLADDER cancer - Abstract
Introduction: The intricate roles of immune cells and inflammatory factors in cancer, particularly their association with the risk of bladder cancer, are not well understood. Methods: This study aimed to clarify potential causal relationships between these elements and the development of bladder cancer using genome-wide association study (GWAS) summary statistics for 731 immune cell phenotypes and 91 circulating inflammatory factors (cases=2,053; controls=287,137). The primary analytical approach was Inverse Variance Weighting (IVW), supplemented by MR-Egger regression, weighted median, and weighted mode analyses. Sensitivity analyses included Cochran Q test, MR-Egger intercept test, and Leave-one-out test. Results: The findings indicated that monocytes are positively correlated with an increased risk of bladder cancer. On the contrary, double-negative (DN) T cells, HLA DR+CD8br, and CD28 on CD28+CD45RA+CD8br T cells exhibited an inverse correlation, suggesting a possible protective effect. Furthermore, inflammatory factors IL-20, IL-22RA1, and Eotaxin were significantly associated with an increased risk of bladder cancer. Discussion: These results suggest that certain immune cell phenotypes and inflammatory factors may play a role in the development of bladder cancer and could serve as potential biomarkers for assessing tumor risk. The findings also offer new insights into the pathogenesis of bladder cancer, indicating a need for further investigation. [ABSTRACT FROM AUTHOR]
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- 2024
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20. Venous thromboembolism and ovarian cancer risk: a Mendelian randomized study.
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Liu, Xiaolin, Wang, Shan, Lv, Hongwei, Chen, Enli, and Yu, Jing
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THROMBOEMBOLISM ,GENOME-wide association studies ,CARCINOGENESIS ,DISEASE risk factors ,LIPID metabolism ,OVARIAN cancer - Abstract
Introduction: A potential link between venous thromboembolism and the risk of ovarian cancer has been identified in clinical practice. However, it is unclear whether there is a causal relationship between the two. In this study, we applied a univariate two-sample Mendelian randomization method to explain the possible link between venous thromboembolism and ovarian cancer pathogenesis at the genetic level, and pointed out that lipid metabolism and ovarian cancer pathogenesis have innovative basic experimental directions. Objective: This study explored the causal effect between a history of venous thromboembolism and the risk of ovarian cancer. Methods: Genome-Wide Association Study (GWAS) data of venous thromboembolism patients (n = 9176) of the same ethnicity were selected as study exposures, and GWAS data of ovarian cancer patients (n = 1218) of the same ethnicity were selected as study exposures. In this study, univariate two-sample Mendelian randomization analysis (UVMR) was performed separately using inverse variance weighted (IVW), MR-Egger regression, and weighted median (WM) to assess causal effects. In this study, Cochran's Q test, MR-Egger regression intercept term, MR-PRESSO, and leave-one-out method were used for sensitivity analysis to assess the stability and reliability of the results. Results: The GWAS data screened in this study were all European ethnicity data. In this study, we found that genetically predicted history of venous thromboembolism was associated with an upward trend in ovarian cancer incidence, and the results of Weighted median, Simple mode, Weighted mode, and MR Egger showed a similar trend (OR = 1.0006, 95% CI: 1.00007–1.0013, p < 0.05). There was no heterogeneity of results (p = 0.18) and no horizontal pleiotropy (p = 0.77). The instrumental variables selected for venous thromboembolism in this study were all strong instrumental variables (F = 669.7). The results of the sensitivity analysis remained consistent. Conclusion: The results of this study indicate that patients with a history of venous thromboembolism are at increased risk of developing ovarian cancer and point to possible associations between lipid metabolism genes, such as CYP4V2, and the development of ovarian cancer, which provide interesting directions for further basic research. [ABSTRACT FROM AUTHOR]
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- 2024
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21. Emerging roles of cancer-associated histone mutations in genomic instabilities.
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Yadav, Priyanka, Jain, Ronit, and Yadav, Rajesh Kumar
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DNA repair ,CELLULAR evolution ,POST-translational modification ,DEOXYRIBOZYMES ,CARCINOGENESIS ,EPIGENOMICS - Abstract
Epigenetic mechanisms often fuel the quick evolution of cancer cells from normal cells. Mutations or aberrant expressions in the enzymes of DNA methylation, histone post-translational modifications, and chromatin remodellers have been extensively investigated in cancer pathogenesis; however, cancer-associated histone mutants have gained momentum in recent decades. Next-generation sequencing of cancer cells has identified somatic recurrent mutations in all the histones (H3, H4, H2A, H2B, and H1) with different frequencies for various tumour types. Importantly, the wellcharacterised H3K27M, H3G34R/V, and H3K36M mutations are termed as oncohistone mutants because of their wide roles, from defects in cellular differentiation, transcriptional dysregulation, and perturbed epigenomic profiles to genomic instabilities. Mechanistically, these histone mutants impart their effects on histone modifications and/or on irregular distributions of chromatin complexes. Recent studies have identified the crucial roles of the H3K27M and H3G34R/V mutants in the DNA damage response pathway, but their impacts on chemotherapy and tumour progression remain elusive. In this review, we summarise the recent developments in their functions toward genomic instabilities and tumour progression. Finally, we discuss how such a mechanistic understanding can be harnessed toward the potential treatment of tumours harbouring the H3K27M, H3G34R/V, and H3K36M mutations. [ABSTRACT FROM AUTHOR]
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- 2024
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22. Ultraviolet (UV) radiation: a double-edged sword in cancer development and therapy.
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Yu, Zhen-wei, Zheng, Min, Fan, Hua-yang, Liang, Xin-hua, and Tang, Ya-ling
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MERKEL cell carcinoma ,CANCER cell growth ,CONTROLLED release drugs ,ULTRAVIOLET radiation ,BASAL cell carcinoma ,SKIN cancer - Abstract
It has long been widely acknowledged that ultraviolet (UV) light is an environment risk factor that can lead to cancer, particularly skin cancer. However, it is worth noting that UV radiation holds potential for cancer treatment as a relatively high-energy electromagnetic wave. With the help of nanomaterials, the role of UV radiation has caught increasing attention in cancer treatment. In this review, we briefly summarized types of UV-induced cancers, including malignant melanoma, squamous cell carcinoma, basal cell carcinoma, Merkel cell carcinoma. Importantly, we discussed the primary mechanisms underlying UV carcinogenesis, including mutations by DNA damage, immunosuppression, inflammation and epigenetic alterations. Historically limited by its shallow penetration depth, the introduction of nanomaterials has dramatically transformed the utilization of UV light in cancer treatment. The direct effect of UV light itself generally leads to the suppression of cancer cell growth and the initiation of apoptosis and ferroptosis. It can also be utilized to activate photosensitizers for reactive oxygen species (ROS) production, sensitize radiotherapy and achieve controlled drug release. Finally, we comprehensively weigh the significant risks and limitations associated with the therapeutic use of UV radiation. And the contradictory effect of UV exposure in promoting and inhibiting tumor has been discussed. This review provides clues for potential clinical therapy as well as future study directions in the UV radiation field. The precise delivery and control of UV light or nanomaterials and the wavelength as well as dose effects of UV light are needed for a thorough understanding of UV radiation. [ABSTRACT FROM AUTHOR]
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- 2024
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23. RABIF promotes hepatocellular carcinoma progression through regulation of mitophagy and glycolysis.
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Feng, Ning, Zhang, Rui, Wen, Xin, Wang, Wei, Zhang, Nie, Zheng, Junnian, Zhang, Longzhen, and Liu, Nianli
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GUANINE nucleotide exchange factors , *REACTIVE oxygen species , *HEPATOCELLULAR carcinoma , *CARCINOGENESIS , *CELL growth - Abstract
The RAB interacting factor (RABIF) is a putative guanine nucleotide exchange factor that also functions as a RAB-stabilizing holdase chaperone. It has been implicated in pathogenesis of several cancers. However, the functional role and molecular mechanism of RABIF in hepatocellular carcinoma (HCC) are not entirely known. Here, we demonstrate an upregulation of RABIF in patients with HCC, correlating with a poor prognosis. RABIF inhibition results in decreased HCC cell growth both in vitro and in vivo. Our study reveals that depleting RABIF attenuates the STOML2-PARL-PGAM5 axis-mediated mitophagy. Consequently, this reduction in mitophagy results in diminished mitochondrial reactive oxygen species (mitoROS) production, thereby alleviating the HIF1α-mediated downregulation of glycolytic genes HK1, HKDC1, and LDHB. Additionally, we illustrate that RABIF regulates glucose uptake by controlling RAB10 expression. Importantly, the knockout of RABIF or blockade of mitophagy sensitizes HCC cells to sorafenib. This study uncovers a previously unrecognized role of RABIF crucial for HCC growth and identifies it as a potential therapeutic target. RABIF upregulation promotes HCC growth, mitophagy, and glycolysis, and enhances sorafenib resistance, suggesting it as a potential therapeutic target. [ABSTRACT FROM AUTHOR]
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- 2024
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24. Potential Role of APC Mutations in the Prognosis and Targeted Therapy of Gastric Adenocarcinoma.
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Zhang, Cao, Qin, Jingjing, Zhou, Wenjuan, Huang, Zexuan, Ye, Jingjing, He, Yaqin, and Sahgal, Pranshu
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ADENOCARCINOMA , *STOMACH tumors , *GENOMICS , *KILLER cells , *RESEARCH funding , *T cells , *CELL proliferation , *CANCER patients , *GENE expression , *ONCOGENES , *METABOLISM , *ADENOMATOUS polyposis coli , *GENETIC mutation , *HUMAN genome , *CARCINOGENESIS , *MOLECULAR biology , *DISEASE progression , *EOSINOPHILS - Abstract
Background: Adenomatous polyposis coli (APC) gene, an oncogene, has been implicated in stomach adenocarcinoma (STAD), which is a common type of gastric cancer (GC). Although the relationship between APC gene mutations and gastric adenocarcinoma has been comprehensively studied, the potential role of these mutations in the prognosis and targeted therapy remains known. Methods: We utilized The Cancer Genome Atlas (TCGA) database to obtain gene expression matrices, clinical information, and mutation data from patients with STAD. The mutation status of the APC gene was analyzed, and its correlation with tumor mutational burden (TMB), microsatellite instability (MSI), and clinical prognosis in STAD was investigated. Gene set enrichment analysis (GSEA) was conducted to explore the pathological role of APC gene mutations in STAD metabolic pathways. Drug sensitivity analysis was conducted to identify potential targeted antitumor drugs for patients with APC gene mutations in gastric adenocarcinoma. Results: The results revealed that 88% (46/52) of STAD samples had nonsynonymous mutations. The mutation group exhibited a significantly higher TMB than the wild‐type group (p < 0.001), and the percentage of high MSI (MSI‐H) was significantly higher in the mutation group than in the wild‐type group (p < 0.001). Patients with APC mutations had a worse prognosis than those with APC wild‐type (p = 0.009). The APC gene mutation group displayed significant enrichment in amino acids, RNA, and several pathways (|NES| > 1 and nominal p value < 0.01). Compared to the wild‐type group, the mutation group exhibited a higher infiltration proportion of natural killer (NK) cells resting and eosinophils, whereas a lower infiltration proportion of monocytes and resting mast cells (p value < 0.05). AZD5991 exhibited significant sensitivity in patients with STAD carrying APC mutations (p = 0.028). Conclusion:APC gene mutations play a crucial role in the prognosis, molecular characteristics, and potential therapeutic strategies for gastric adenocarcinoma. [ABSTRACT FROM AUTHOR]
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- 2024
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25. Don't fear the reaper: The role of regulated cell death in tumorigenesis and BH3-mimetics for cancer therapy.
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La Marca, John E., Kelly, Gemma L., Strasser, Andreas, and Diepstraten, Sarah T.
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APOPTOSIS , *CELL death , *CANCER cells , *CARCINOGENESIS , *CELLULAR therapy - Abstract
From its earliest characterization, it has been recognized that there is a role for regulated (programmed) cell death in cancer. As our understanding of the different types of programmed cell death processes and their molecular control has advanced, so have the technologies that allow us to manipulate these processes to, for example, fight against cancer. In this review, we describe the roles of the different forms of regulated cell death in the development of cancer as well as their potential therapeutic exploitation. In that vein, we explore the development and use of BH3-mimetics, a unique class of drugs that can directly activate the apoptotic cell death machinery to treat cancer. Finally, we address key challenges that face the field to improve the use of these therapeutics and the efforts that are being undertaken to do so. In this review, La Marca et al. delve into the role of cell death in tumorigenesis; particularly apoptosis, but also touching on emerging roles for other cell death variants. The review also discusses the development, limitations, and future of BH3-mimetics, the most clinically advanced class of compounds for direct apoptosis induction. [ABSTRACT FROM AUTHOR]
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- 2024
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26. Unraveling TRPV1's Role in Cancer: Expression, Modulation, and Therapeutic Opportunities with Capsaicin.
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Chinreddy, Subramanyam R., Mashozhera, Nicole Tendayi, Rashrash, Badraldeen, Flores-Iga, Gerardo, Nimmakayala, Padma, Hankins, Gerald R., Harris, Robert T., and Reddy, Umesh K.
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TRPV cation channels , *CELL communication , *CANCER pain , *CARCINOGENESIS , *PEPPERS , *HOT peppers - Abstract
Cancer is a global health challenge with rising incidence and mortality rates, posing significant concerns. The World Health Organization reports cancer as a leading cause of death worldwide, contributing to nearly one in six deaths. Cancer pathogenesis involves disruptions in cellular signaling pathways, resulting in uncontrolled cell growth and metastasis. Among emerging players in cancer biology, Transient Receptor Potential (TRP) channels, notably TRPV1, have garnered attention due to their altered expression in cancer cells and roles in tumorigenesis and progression. TRPV1, also known as the capsaicin receptor, is pivotal in cancer cell death and pain mediation, offering promise as a therapeutic target. Activation of TRPV1 triggers calcium influx and affects cell signaling linked to growth and death. Additionally, TRPV1 is implicated in cancer-induced pain and chemo-sensitivity, with upregulation observed in sensory neurons innervating oral cancers. Also, when capsaicin, a compound from chili peppers, interacts with TRPV1, it elicits a "hot" sensation and influences cancer processes through calcium influx. Understanding TRPV1's multifaceted roles in cancer may lead to novel therapeutic strategies for managing cancer-related symptoms and improving patient outcomes. The current review elucidates the comprehensive role of capsaicin in cancer therapy, particularly through the TRPV1 channel, highlighting its effects in various cells via different signaling pathways and discussing its limitations. [ABSTRACT FROM AUTHOR]
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- 2024
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27. Circulating RKIP and pRKIP in Early-Stage Lung Cancer: Results from a Pilot Study.
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Gasparri, Roberto, Papale, Massimo, Sabalic, Angela, Catalano, Valeria, Deleonardis, Annamaria, De Luca, Federica, Ranieri, Elena, and Spaggiari, Lorenzo
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PROTEIN kinase inhibitors , *COMPUTED tomography , *TUMOR markers , *LUNG cancer , *CARCINOGENESIS - Abstract
Background: Lung cancer (LC) is the leading cause of cancer-related deaths. Although low-dose computed tomography (LD-CT) reduces mortality, its clinical use is limited by cost, radiation, and false positives. Therefore, there is an urgent need for non-invasive and cost-effective biomarkers. The Raf Kinase Inhibitor Protein (RKIP) plays a crucial role in cancer development and progression and may also contribute to regulating the tumor–immune system axis. This protein has recently been described in biological fluids. Therefore, we conducted a pilot case–control study to assess RKIP and phosphorylated RKIP (pRKIP) levels in the urine and blood of LC patients. Methods: A novel enzyme linked immunosorbent assay (ELISA) assay was used to measure RKIP and pRKIP levels in urine and blood samples of two cohorts of LC patients and healthy controls (HSs). Furthermore, the biomarkers levels were correlated with tumor characteristics. Results: Serum, but not urine, levels of RKIP were significantly elevated in LC patients, distinguishing them from low- and high-risk healthy subjects with 93% and 74% accuracy, respectively. The RKIP/pRKIP ratio (RpR score) showed an accuracy of 90% and 79% in distinguishing LC patients from HS and HR-HS, respectively. Additionally, the RpR score correlated better with dimension, stage, and lymph node involvement in the tumor group. Conclusions: The serum RKIP and pRKIP profile may be a promising novel biomarker for early-stage LC. [ABSTRACT FROM AUTHOR]
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- 2024
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28. The Clinical Significance of Pancreatic Steatosis in Pancreatic Cancer: A Hospital-Based Study.
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Chan, Chia-Hao, Chang, Chia-Chen, and Peng, Yen-Chun
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MAGNETIC resonance imaging , *PANCREATIC cancer , *FATTY liver , *DISEASE risk factors , *CARCINOGENESIS - Abstract
Background/Objectives: Pancreatic cancer remains one of the deadliest malignancies worldwide with a pressing need for early detection and intervention strategies. Emerging evidence has suggested a potential link between pancreas steatosis, characterized by excessive pancreatic fat accumulation, and an increased risk of pancreatic cancer development. This retrospective imaging study aims to elucidate the association between pancreatic steatosis and the subsequent development of pancreatic cancer. In the study, we aimed to determine the characteristics of pancreatic cancer with pancreatic steatosis. Methods: During the period of January 2022 to December 2022, we conducted a retrospective study, collecting 101 newly diagnosed pancreas cancer cases from the available image datasets. A comprehensive database of retrospective abdominal imaging studies, comprising computed tomography (CT) and magnetic resonance imaging (MRI), was established from a diverse patient population and subsequently analyzed. Inclusion criteria encompassed patients having available baseline imaging data, allowing for the assessment of pancreatic fat content. Pancreatic fat content was quantified using validated radiological techniques, while demographic, clinical, and histopathological data were all collected. The clinical data and patient characteristics were collected from medical records and analyzed. Results: Preliminary analysis revealed a significant correlation between elevated pancreatic fat content and an increased incidence of subsequent pancreatic cancer. Moreover, subgroup analysis based on age, gender, and comorbidities provided valuable insight into potential risk factors associated with this progression. Additionally, the study identified novel radiological markers that may serve as early indicators of pancreatic cancer development in individuals with pancreatic steatosis. Conclusions: In the imaging study, approximately 30% (30/101) of pancreatic cancer patients presented with pancreatic steatosis. Chronic pancreatitis emerged as the primary factor contributing to pancreatic steatosis in these patients. Importantly, pancreatic steatosis did not significantly impact the prognosis of pancreatic cancer. Follow-up data revealed no significant differences in survival duration between patients with or without pancreatic steatosis. Additionally, no association was found between pancreatic steatosis and hepatic steatosis. [ABSTRACT FROM AUTHOR]
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- 2024
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29. Exploring DIX-DIX Homo- and Hetero-Oligomers in Wnt Signaling with AlphaFold2.
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Wen, Zehua, Wang, Lei, Liu, Shi-Wei, Fan, Hua-Jun Shawn, Song, Jong-Won, and Lee, Ho-Jin
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WNT signal transduction , *CELLULAR signal transduction , *HOMODIMERS , *CARCINOGENESIS , *SIGNALS & signaling - Abstract
Wnt signaling is involved in embryo development and cancer. The binding between the DIX domains of Axin1/2, Dishevelled1/2/3, and Coiled-coil-DIX1 is essential for Wnt/β-catenin signaling. Structural and biological studies have revealed that DIX domains are polymerized through head-to-tail interface interactions, which are indispensable for activating β-catenin Wnt signaling. Although different isoforms of Dvl and Axin proteins display both redundant and specific functions in Wnt signaling, the specificity of DIX-mediated interactions remains unclear due to technical challenges. Using AlphaFold2(AF2), we predict the structures of 6 homodimers and 22 heterodimers of DIX domains without templates and compare them with the reported X-ray complex structures. PRODIGY is used to calculate the binding affinities of these DIX complexes. Our results show that the Axin2 DIX homodimer has a stronger binding affinity than the Axin1 DIX homodimer. Among Dishevelled (Dvl) proteins, the binding affinity of the Dvl1 DIX homodimer is stronger than that of Dvl2 and Dvl3. The Coiled-coil-DIX1(Ccd1) DIX homodimer shows weaker binding than the Axin1 DIX homodimer. Generally, heterodimer interactions tend to be stronger than those of homodimers. Our findings provide insights into the mechanism of the Wnt signaling pathway and highlight the potential of AF2 and PRODIGY for studying protein–protein interactions in signaling pathways. [ABSTRACT FROM AUTHOR]
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- 2024
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30. From Cancer to Immune Organoids: Innovative Preclinical Models to Dissect the Crosstalk between Cancer Cells and the Tumor Microenvironment.
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Picca, Francesca, Giannotta, Claudia, Tao, Jiahao, Giordanengo, Lucia, Munir, H. M. Waqas, Botta, Virginia, Merlini, Alessandra, Mogavero, Andrea, Garbo, Edoardo, Poletto, Stefano, Bironzo, Paolo, Doronzo, Gabriella, Novello, Silvia, Taulli, Riccardo, and Bersani, Francesca
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MICROPHYSIOLOGICAL systems , *MEDICAL research , *TUMOR classification , *TUMOR microenvironment , *CARCINOGENESIS - Abstract
Genomic-oriented oncology has improved tumor classification, treatment options, and patient outcomes. However, genetic heterogeneity, tumor cell plasticity, and the ability of cancer cells to hijack the tumor microenvironment (TME) represent a major roadblock for cancer eradication. Recent biotechnological advances in organotypic cell cultures have revolutionized biomedical research, opening new avenues to explore the use of cancer organoids in functional precision oncology, especially when genomics alone is not a determinant. Here, we outline the potential and the limitations of tumor organoids in preclinical and translational studies with a particular focus on lung cancer pathogenesis, highlighting their relevance in predicting therapy response, evaluating treatment toxicity, and designing novel anticancer strategies. Furthermore, we describe innovative organotypic coculture systems to dissect the crosstalk with the TME and to test the efficacy of different immunotherapy approaches, including adoptive cell therapy. Finally, we discuss the potential clinical relevance of microfluidic mini-organ technology, capable of reproducing tumor vasculature and the dynamics of tumor initiation and progression, as well as immunomodulatory interactions among tumor organoids, cancer-associated fibroblasts (CAFs) and immune cells, paving the way for next-generation immune precision oncology. [ABSTRACT FROM AUTHOR]
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- 2024
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31. SSCI: Self-Supervised Deep Learning Improves Network Structure for Cancer Driver Gene Identification.
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Xu, Jialuo, Hao, Jun, Liao, Xingyu, Shang, Xuequn, and Li, Xingyi
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CANCER genes , *RECEIVER operating characteristic curves , *EARLY detection of cancer , *DEEP learning , *CARCINOGENESIS , *BIOLOGICAL networks - Abstract
The pathogenesis of cancer is complex, involving abnormalities in some genes in organisms. Accurately identifying cancer genes is crucial for the early detection of cancer and personalized treatment, among other applications. Recent studies have used graph deep learning methods to identify cancer driver genes based on biological networks. However, incompleteness and the noise of the networks will weaken the performance of models. To address this, we propose a cancer driver gene identification method based on self-supervision for graph convolutional networks, which can efficiently enhance the structure of the network and further improve predictive accuracy. The reliability of SSCI is verified by the area under the receiver operating characteristic curves (AUROC), the area under the precision-recall curves (AUPRC), and the F1 score, with respective values of 0.966, 0.964, and 0.913. The results show that our method can identify cancer driver genes with strong discriminative power and biological interpretability. [ABSTRACT FROM AUTHOR]
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- 2024
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32. Interplay of Cardiometabolic Syndrome and Biliary Tract Cancer: A Comprehensive Analysis with Gender-Specific Insights.
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Di Stasi, Vincenza, Contaldo, Antonella, Birtolo, Lucia Ilaria, and Shahini, Endrit
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LIVER disease diagnosis , *TREATMENT of diabetes , *LIVER disease prevention , *RISK assessment , *NON-alcoholic fatty liver disease , *WEIGHT loss , *CARDIOVASCULAR diseases , *CHOLANGIOCARCINOMA , *SEX distribution , *CARDIOVASCULAR diseases risk factors , *CHRONIC diseases , *METABOLIC syndrome , *TYPE 2 diabetes , *EARLY diagnosis , *VIRUS diseases , *DISEASE progression , *COMORBIDITY , *OBESITY , *BIOMARKERS , *SEQUENCE analysis , *DISEASE risk factors ,BILE duct tumors - Abstract
Simple Summary: Metabolic syndrome (MetS), metabolic dysfunction-associated steatotic liver disease (MASLD), and diabetes are all linked to Cholangiocarcinoma (CCA) in various ways. MASLD may have an increased risk of intrahepatic-CCA, whereas untreated patients with shorter diabetes durations were more likely to develop biliary tract cancer (BTC). More research is needed to understand how reproductive hormones cause BTC. BTC patients may be at increased intrinsic cardiovascular risk of neoplastic/non-neoplastic cardiac complications. Therefore, early detection/prevention of chronic liver disease, as well as intervention studies, will almost certainly be required to determine whether improvements in MetS, weight loss, and diabetes therapy can reduce CCA risk and progression. BTC overall incidence is globally increasing. CCA, including its subtypes, is a form of BTC. MetS, obesity, MASLD, and diabetes are all linked to CCA in interconnected ways. The link between obesity and CCA is less well-defined in Eastern countries as compared to Western. Although more research is needed to determine the relationship between MASLD and extrahepatic CCA (eCCA), MASLD may be a concurrent risk factor for intrahepatic CCA, particularly in populations with established or unidentified underlying liver disease. Interestingly, the risk of biliary tract cancer (BTC) seemed to be higher in patients with shorter diabetes durations who were not treated with insulin. Therefore, early detection and prevention of chronic liver disease, as well as additional intervention studies, will undoubtedly be required to determine whether improvements to MetS, weight loss, and diabetes therapy can reduce the risk and progression of BTC. However, further studies are needed to understand how reproductive hormones are involved in causing BTC and to develop consistent treatment for patients. Finally, it is critical to carefully assess the cardiological risk in BTC patients due to their increased intrinsic cardiovascular risk, putting them at risk for thrombotic complications, cardiovascular death, cardiac metastasis, and nonbacterial thrombotic endocarditis. This review aimed to provide an updated summary of the relation between the abovementioned cardio-metabolic conditions and BTC. [ABSTRACT FROM AUTHOR]
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- 2024
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33. Altered Microbiome Promotes Pro-Inflammatory Pathways in Oesophago-Gastric Tumourigenesis.
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Patel, Nikhil Manish, Patel, Pranav Harshad, Bhogal, Ricky Harminder, Harrington, Kevin Joseph, Singanayagam, Aran, and Kumar, Sacheen
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STOMACH tumors , *NEOPLASTIC cell transformation , *GENOMICS , *EARLY detection of cancer , *ESOPHAGEAL tumors , *HUMAN microbiota , *CELLULAR signal transduction , *AGE distribution , *INFLAMMATION , *CARCINOGENESIS , *NATURAL immunity , *GASTROESOPHAGEAL reflux , *DRUG utilization , *DISEASE risk factors - Abstract
Simple Summary: Cancer of the upper digestive system is associated with poor survival due to difficulties in diagnosing the disease early, before it has spread around the body. Previous research has shown that the healthy bacteria within the body changes in response to medications, diet and infection. Some of these changes are associated with a greater risk of developing cancers of the oesophagus (gullet) and stomach. This occurs, at least in part, through inflammation. This process is the body's natural response to infection, injury and exposure to potentially harmful substances, which, if uncontrolled can lead to diseases including cancer. This review article aims to explain the ways in which this happens. By developing a deeper understanding of these processes, advances in early diagnosis of this disease can be made, which may lead to better survival. Introduction: The upper gastrointestinal microbiome is a dynamic entity that is involved in numerous processes including digestion, production of vitamins and protection against pathogens. Many external and intrinsic factors may cause changes in the proportions of bacteria within the microbial community, termed 'dysbiosis'. A number of these have been identified as risk factors for a range of diseases, including oesophago-gastric carcinoma. Materials and Methods: A narrative review was conducted to elucidate the current evidence on the role of the microbiome in promoting oesophago-gastric tumourigenesis. Significant causes of dysbiosis including age, medications and GORD were examined and key pro-inflammatory pathways implicated in tumourigenesis and their interaction with the microbiome were described. Results and Discussion: An association between microbial dysbiosis and development of oesophago-gastric cancer may be mediated via activation of pro-inflammatory pathways, the inflammasome and the innate immune system. Advances in sequencing technology allow microbial communities to be fingerprinted by sequencing the 16S rRNA gene, enabling a deeper understanding of the genera that may be implicated in driving tumourigenesis. Conclusions: Developing a greater understanding of the influence of the microbiota on oesophago-gastric tumourigenesis may enable advances to be made in the early detection of malignancy and in the development of novel systemic therapies, leading to improved rates of survival. [ABSTRACT FROM AUTHOR]
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- 2024
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34. Role of Exosomes in Salivary Gland Tumors and Technological Advances in Their Assessment.
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Nieszporek, Artur, Wierzbicka, Małgorzata, Labedz, Natalia, Zajac, Weronika, Cybinska, Joanna, and Gazinska, Patrycja
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BIOPSY , *MEDICAL technology , *CELL physiology , *SALIVARY gland tumors , *CELLULAR signal transduction , *METASTASIS , *CARCINOGENESIS , *EXOSOMES , *DISEASE progression - Abstract
Simple Summary: Salivary gland tumors (SGTs) are rare and complex, making them difficult to diagnose and treat. This work focuses on the role of exosomes, extracellular vesicles secreted by almost all cell types, in the development and progression of SGT. Exosomes are crucial in cell-to-cell communication and play significant roles in tumor biology, including modulating the tumor environment, aiding metastasis, and affecting immune responses. A better understanding of exosome biology can lead to their use as biomarkers for diagnosis and prognosis, as well as targets for treatment, potentially transforming SGT management and improving patient outcomes. Exosome-based liquid biopsies could offer non-invasive, real-time diagnostics and enhance patient care through precision medicine. This review explores the advancements in salivary exosome analysis, highlighting its potential for non-invasive cancer detection and the development of innovative diagnostic techniques. Backgroud: Salivary gland tumors (SGTs) are rare and diverse neoplasms, presenting significant challenges in diagnosis and management due to their rarity and complexity. Exosomes, lipid bilayer vesicles secreted by almost all cell types and present in all body fluids, have emerged as crucial intercellular communication agents. They play multifaceted roles in tumor biology, including modulating the tumor microenvironment, promoting metastasis, and influencing immune responses. Results: This review focuses on the role of exosomes in SGT, hypothesizing that novel diagnostic and therapeutic approaches can be developed by exploring the mechanisms through which exosomes influence tumor occurrence and progression. By understanding these mechanisms, we can leverage exosomes as diagnostic and prognostic biomarkers, and target them for therapeutic interventions. The exploration of exosome-mediated pathways contributing to tumor progression and metastasis could lead to more effective treatments, transforming the management of SGT and improving patient outcomes. Ongoing research aims to elucidate the specific cargo and signaling pathways involved in exosome-mediated tumorigenesis and to develop standardized techniques for exosome-based liquid biopsies in clinical settings. Conclusions: Exosome-based liquid biopsies have shown promise as non-invasive, real-time systemic profiling tools for tumor diagnostics and prognosis, offering significant potential for enhancing patient care through precision and personalized medicine. Methods like fluorescence, electrochemical, colorimetric, and surface plasmon resonance (SPR) biosensors, combined with artificial intelligence, improve exosome analysis, providing rapid, precise, and clinically valid cancer diagnostics for difficult-to-diagnose cancers. [ABSTRACT FROM AUTHOR]
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- 2024
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35. Clinical Aspects and Significance of β-Chemokines, γ-Chemokines, and δ-Chemokines in Molecular Cancer Processes in Acute Myeloid Leukemia (AML) and Myelodysplastic Neoplasms (MDS).
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Korbecki, Jan, Bosiacki, Mateusz, Stasiak, Piotr, Snarski, Emilian, Brodowska, Agnieszka, Chlubek, Dariusz, and Baranowska-Bosiacka, Irena
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MYELODYSPLASTIC syndromes , *CHEMOKINES , *CELL communication , *DRUG resistance in cancer cells , *MACROPHAGES , *T cells , *CELL proliferation , *ANTINEOPLASTIC agents , *MESENCHYMAL stem cells , *CARCINOGENESIS , *CHEMOKINE receptors - Abstract
Simple Summary: This article examines the significance of β-chemokines, γ-chemokines, and δ-chemokines in acute myeloid leukemia (AML). It focuses on the effects of these chemotactic cytokines on both leukemic cells and non-leukemic cells within the tumor niche in the bone marrow. This article emphasizes the substantial impact of certain chemokines on tumorigenic processes in AML, highlighting the correlation between chemokine expression and patient prognosis. However, the mechanisms underlying this relationship remain poorly understood. The lack of comprehensive understanding of the role of chemokines in AML impedes the development of new anti-leukemic drugs targeting these chemokines and their receptors. Background/Objectives: Acute myeloid leukemia (AML) is a type of leukemia with a very poor prognosis. Consequently, this neoplasm is extensively researched to discover new therapeutic strategies. One area of investigation is the study of intracellular communication and the impact of the bone marrow microenvironment on AML cells, with chemokines being a key focus. The roles of β-chemokines, γ-chemokines, and δ-chemokines in AML processes have not yet been sufficiently characterized. Methods: This publication summarizes all available knowledge about these chemotactic cytokines in AML and myelodysplastic neoplasm (MDS) processes and presents potential therapeutic strategies to combat the disease. The significance of β-chemokines, γ-chemokines, and δ-chemokines is detailed, including CCL2 (MCP-1), CCL3 (MIP-1α), CCL5 (RANTES), CCL23, CCL28, and CX3CL1 (fractalkine). Additionally, the importance of atypical chemokine receptors in AML is discussed, specifically ACKR1, ACKR2, ACKR4, and CCRL2. Results/Conclusions: The focus is on the effects of these chemokines on AML cells, particularly their influence on proliferation and resistance to anti-leukemic drugs. Intercellular interactions with non-AML cells, such as mesenchymal stem cells (MSC), macrophages, and regulatory T cells (Treg), are also characterized. The clinical aspects of chemokines are thoroughly explained, including their effect on overall survival and the relationship between their blood levels and AML characteristics. [ABSTRACT FROM AUTHOR]
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- 2024
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36. In Vivo Chemosuppressive Effects of Kolaviron on 7,12-Dimethylbenzanthracene-Induced Mammary Lesions are Associated with Changes in Levels of Estrogen Receptor-α, CYP 1A1, Proinflammatory Cytokines, and Alterations to Metabolic Pathways Implicated in Mammary Carcinogenesis
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Attah, Catherine Ojebbah, Alhaji, Umar Ismail, Ameh, Danladi Amodu, Forcados, Gilead Ebiegberi, Muhammad, Aliyu, Bashir, Musa, and Ibrahim, Sani
- Subjects
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TYROSINE metabolism , *RESEARCH funding , *ANTINEOPLASTIC agents , *BREAST tumors , *CELLULAR signal transduction , *IN vivo studies , *PLANT extracts , *ESTROGEN receptors , *RATS , *DOSE-effect relationship in pharmacology , *SULFONAMIDES , *CYTOCHROME P-450 , *ANIMAL experimentation , *CYTOKINES , *PHARMACODYNAMICS , *BLOOD ,BREAST tumor prevention - Abstract
Garcinia kola is a medicinal food commonly consumed in Sub-Sahara Africa, for which Kolaviron (KV) is the active portion. As a follow-up to our earlier chemopreventive studies, we investigated the chemotherapeutic effects of KV on experimentally induced mammary carcinogenesis in female Wistar rats. Mammary carcinogenesis was induced using 80 mg/kg of 7,12-dimethylbenzanthracene (DMBA) administered by oral gavage. One hundred-fifty days post-DMBA induction, estrogen receptor-α (ER-α) levels were determined in the experimental rats before treatment with KV commenced. Treatment was done using 50, 100, and 200 mg/kg KV thrice a week for 4 weeks, after which the experiment was terminated. Significantly higher levels of estrogen receptor-α, CYP 1A1, malondialdehyde, formation of lobular neoplastic cells, epithelial hyperplasia, lymphocyte infiltration, and increased cytokine (interleukin-6 and tumor necrosis factor-α) activity were observed in DMBA-induced rats, which were attenuated in KV-treated rats. Tyrosine metabolism was exclusively enriched in DMBA-induced rats in contrast to KV-treated rats. Collectively, the results point to the chemotherapeutic potential of KV. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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37. Regular exercise suppresses steatosis‐associated liver cancer development by degrading E2F1 and c‐Myc via circadian gene upregulation.
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Huyen, Vu Thuong, Echizen, Kanae, Yamagishi, Ryota, Kumagai, Miho, Nonaka, Yoshiki, Kodama, Takahiro, Ando, Tatsuya, Yano, Megumu, Takada, Naoki, Takasugi, Masaki, Kamachi, Fumitaka, and Ohtani, Naoko
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LIVER cancer , *GENE expression profiling , *CARCINOGENESIS , *CELL proliferation , *CANCER prevention , *CIRCADIAN rhythms - Abstract
Regular exercise is believed to suppress cancer progression. However, the precise molecular mechanisms by which exercise prevents cancer development remain unclear. In this study, using a steatosis‐associated liver cancer mouse model, we found that regular exercise at a speed of 18 m/min for 20 min daily suppressed liver cancer development. To explore the underlying mechanisms, we examined the gene expression profiles in the livers of the exercise and non‐exercise groups. The expressions of circadian genes, such as Per1 and Cry2, were upregulated in the exercise group. As circadian rhythm disruption is known to cause various diseases, including cancer, improving circadian rhythm through exercise could contribute to cancer prevention. We further found that the expression of a series of E2F1 and c‐Myc target genes that directly affect the proliferation of cancer cells was downregulated in the exercise group. However, the expression of E2F1 and c‐Myc was transcriptionally unchanged but degraded at the post‐translational level by exercise. Cry2, which is regulated by the Skp1‐Cul1‐FBXL3 (SCFFBXL3) ubiquitin ligase complex by binding to FBXL3, can form a complex with E2F1 and c‐Myc, which we think is the mechanism to degrade them. Our study revealed a previously unknown mechanism by which exercise prevents cancer development. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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38. Vitamin D Significantly Inhibits Carcinogenesis in the Mogp-TAg Mouse Model of Fallopian Tube Ovarian Cancer.
- Author
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Nelson, Omar L., Rosales, Rebecca, Turbov, Jane, Thaete, Larry G, Balamayooran, Gayathriy, Cline, J Mark, Pike, J. Wesley, and Rodriguez, Gustavo C.
- Abstract
Epidemiological and observational studies suggest that vitamin D has potential for the chemoprevention of ovarian cancer. The anticancer effect of vitamin D in the fallopian tube epithelium (FTE), which is now thought to harbor the precursor cells for high grade ovarian cancer, is not known. The purpose of this study was to investigate whether vitamin D can inhibit carcinogenesis in the mogp-TAg fallopian tube (FT) ovarian cancer mouse model and examine underlying mechanisms. To test this hypothesis, 3 groups of 40 5-week-old female mogp-TAg mice were divided equally into two cohorts of 20 mice, treated with either vehicle (vitamin D solvent) or the active 1,25(OH)2D3 analogue EB1089, delivered via mini-pump or IP injection or cholecalciferol delivered in the feed. The FTs were characterized histologically and pathologically after 3 and 7 weeks of treatment. The effect of vitamin D on cultured human FTE cells was also examined. After 3 weeks, vitamin D, delivered as either cholecalciferol or EB1089 significantly inhibited FT carcinogenesis. After 7 weeks, cholecalciferol significantly reduced p53 signatures, serous tubal epithelial carcinoma, FT cancer, and plasma CA125 while increasing apoptosis in the FTE. EB1089 had no significant effect on FT carcinogenesis at 7 weeks. Cholecalciferol significantly reduced proliferation and increased apoptosis in vitro in p53-altered FTE cells. In conclusion, vitamin D inhibited FT carcinogenesis by clearing cells with p53 alterations. These data suggest that vitamin D has merit for the chemoprevention of fallopian tube/ovarian cancer. The optimal chemopreventive effect may be dependent on the route of vitamin D administration [ABSTRACT FROM AUTHOR]
- Published
- 2024
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39. The Influence of Removable Complete Denture on Pro‐Oxidant Antioxidant Balance and Redox‐Sensitive Inflammation Biomarker NF‐ĸB in the Oral Cavity: An Interventional Follow‐Up Study.
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Bošković, Mirjana, Sokolović, Dušan, Stanković, Saša, Ristić, Ivan, Popović, Jordan, and Kocić, Gordana
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COMPLETE dentures ,TRANSCRIPTION factors ,CLINICAL trials ,INFLAMMATORY mediators ,REACTIVE oxygen species ,ORAL lichen planus - Abstract
Objectives: Oxidative stress, an imbalance between the body's natural antioxidant defenses and the production of reactive oxygen species (ROS), can result in serious oral diseases, including oral cancer, periodontal diseases, and oral lichen planus, through the activation of the redox‐sensitive transcription factors and inflammation. The purpose of this study was to assess the potential effects of a removable complete denture on the levels of oxidative stress markers, such as lipid peroxidation (MDA), advanced oxidation protein products (AOPP), and catalase, and the quantitative expression of the redox‐sensitive transcription factor NF‐κB p65 subunit. Materials and Methods: This interventional follow‐up study enrolled 40 participants of both sexes aged 28–78 years, with a median age of 56 years, where unstimulated saliva was collected before denture placement, immediately after the denture placement, and 24 h, 7 days, and 30 days after the denture placement. The most prominent ROS overproduction was reported on the seventh day (p < 0.05), followed by a significant fall in antioxidative defense. Results: The NF‐κB p65 subunit, whose expression pattern was highest in the same time period on the seventh day, serves as a signaling molecule for redox imbalance due to ROS production. Over the next 30 days, its levels remained moderately increased compared to the basal value, which may influence pro‐inflammatory pathways and the integrity of oral tissue components. These alterations may be induced by the dentures, which can produce high pressures on the supporting tissues or by the synthetic materials used for producing the dentures. Conclusion: Our research may help to clarify the potential pathways by which oxidative stress and redox‐sensitive inflammatory mediators, as well as mechanical and chemical irritants, may serve as risk factors for premalignant lesions in the mouth. Further research on this topic is required to understand the molecular mechanisms behind the relationship between inflammation and oral premalignant lesions caused by mechanical and chemical irritation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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40. Reading the m6A-encoded epitranscriptomic information in development and diseases.
- Author
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Chen, Yunbing, Zhou, Ziyu, Chen, Yanxi, and Chen, Di
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RNA modification & restriction , *CARCINOGENESIS , *EPIGENETICS , *RNA , *PROTEINS - Abstract
N6-methyladenosine (m6A) represents the most prevalent internal and reversible modification on RNAs. Different cell types display their unique m6A profiles, which are determined by the functions of m6A writers and erasers. M6A modifications lead to different outcomes such as decay, stabilization, or transport of the RNAs. The m6A-encoded epigenetic information is interpreted by m6A readers and their interacting proteins. M6A readers are essential for different biological processes, and the defects in m6A readers have been discovered in diverse diseases. Here, we review the latest advances in the roles of m6A readers in development and diseases. These recent studies not only highlight the importance of m6A readers in regulating cell fate transitions, but also point to the potential application of drugs targeting m6A readers in diseases. [ABSTRACT FROM AUTHOR]
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- 2024
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41. The role of the ceRNA network mediated by lncRNA SNHG3 in the progression of cancer.
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Peng, Ying and Long, Xi-Dai
- Subjects
COMPETITIVE endogenous RNA ,LINCRNA ,BASE pairs ,NON-coding RNA ,CARCINOGENESIS - Abstract
Background: Long non-coding RNAs (lncRNAs) are a distinct class of RNAs with longer than 200 base pairs that are not translated into proteins. Small Nucleolar RNA Host Gene 3 (SNHG3) is a lncRNA and frequently dysregulated in various human cancers. Objective: This review provides a comprehensive analysis of current research on lncRNA SNHG3, focusing on its role within the competitive endogenous RNA (ceRNA) network and its implications in cancer. Methods: A systematic literature review was conducted using PubMed up to October 2023. The search strategy included keywords such as "lncRNA SNHG3", "competitive endogenous RNA", "cancer", and related terms. Studies were selected based on relevance to SNHG3's involvement in cancer pathogenesis and progression. Results: Disruptions in the ceRNA network involving lncRNA SNHG3 can impair normal cell growth and differentiation, significantly contributing to disease pathogenesis, particularly cancer. This review highlights SNHG3's substantial impact on various cancer processes and its potential as a diagnostic and therapeutic tool for aggressive cancers. Conclusion: The findings underscore SNHG3's pivotal role in cancer prevention, diagnosis, and treatment, laying a foundation for future research in cancer management. Insights from this review emphasize the necessity for further exploration and development of SNHG3-based diagnostic and therapeutic strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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42. Endocan, a novel glycoprotein with multiple biological activities, may play important roles in neurological diseases.
- Author
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Shuo Liu, Tao Bai, and Juan Feng
- Subjects
CARDIOVASCULAR diseases ,ISCHEMIA ,REPERFUSION injury ,CELL proliferation ,EPIGENOMICS ,HYPERTENSION ,GLYCOPROTEINS ,CELLULAR signal transduction ,ATHEROSCLEROSIS ,NEURODEGENERATION ,NEUROINFLAMMATION ,NEUROLOGICAL disorders ,ENDOTHELIAL cells ,AUTOIMMUNE diseases ,SEPSIS ,MOLECULAR structure ,GROWTH factors ,LIPOPOLYSACCHARIDES ,MOLECULAR biology ,CARCINOGENESIS ,INFLAMMATION ,CYTOKINES ,BIOMARKERS ,DISEASE progression ,DIABETES - Abstract
Endothelial cell specific-1 (ESM-1), also known as endocan, is a soluble dermatan sulfate proteoglycan that is mainly secreted by endothelial cells. Endocan is associated with tumorigenesis and cancer progression and is also related to cardiovascular disorders, autoimmune diseases, and sepsis. The phenylalaninerich region and linear polysaccharide of endocan are necessary for the protein to exert its biological functions. Elevated plasma endocan levels reflect endothelial activation and dysfunction. In addition, endocan participates in complex inflammatory responses and proliferative processes. Here, we reviewed current research on endocan, elaborated the protein's structure and biological functions, and speculated on its possible clinical value in nervous system diseases. We conclude that endocan may be a glycoprotein that plays an important role in neurological disorders. [ABSTRACT FROM AUTHOR]
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- 2024
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43. Identification of BBC3 as a novel indicator for predicting prostate cancer development and olaparib resistance.
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Ma, Junjie, Qin, Xin, Le, Wei, Chen, Xi, Wang, Xiao, and Xu, Chengdang
- Subjects
POLY(ADP-ribose) polymerase ,GENE expression ,OLDER men ,PROSTATE cancer ,CARCINOGENESIS ,GENE ontology - Abstract
Prostate cancer (PCa) is a commonly occurring malignancy in elderly men. Olaparib, a poly ADP-ribose polymerase inhibitor, is utilized in PCa treatment. However, patients often develop resistance to olaparib after a period of treatment. Genetic alterations may play a significant role in this resistance, but the specific genes involved remain unclear. This study collected RNA-sequence data from the Gene Expression Omnibus database on both olaparib-sensitive and -resistant PCa cells to identify genes crucial for resistance. Subsequently, the enriched pathways of these genes were analyzed, and a protein–protein interaction (PPI) network was constructed to identify hub genes. The effect of these hub genes on PCa occurrence, progression, and prognosis was assessed using data from The Cancer Genome Atlas and Chinese Prostate Cancer Genome and Epigenome Atlas databases. Finally, this study validated our findings in clinical PCa samples and cells. From the GSE189186 dataset, 50 upregulated genes and 2 downregulated genes were identified in olaparib-resistant C4-2B and LNCaP cells. Utilizing the PPI network, eight upregulated genes (BBC3, TP53I3, FDXR, DDB2, CDKN1A, GADD45A, ZMAT3, and SESN1) were identified as hub genes for olaparib-resistant PCa cells. Furthermore, some of these genes were central to PCa occurrence, with BBC3 also influencing progression and prognosis. Importantly, BBC3 expression was upregulated in clinical PCa samples and affected PCa cells sensitive to olaparib, suggesting its potential as a predictive marker for PCa development and olaparib resistance. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
44. Overcoming drug resistance of cancer cells by targeting the FGF1/FGFR1 axis with honokiol or FGF ligand trap.
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Szymczyk, Jakub, Sochacka, Martyna, Biadun, Martyna, Sluzalska, Katarzyna Dominika, Witkowska, Danuta, and Zakrzewska, Malgorzata
- Subjects
DRUG resistance in cancer cells ,DRUG resistance ,CARCINOGENESIS ,CANCER cells ,ANTINEOPLASTIC agents ,FIBROBLAST growth factors - Abstract
Background: Chemoresistance of cancer cells, resulting from various mechanisms, is a significant obstacle to the effectiveness of modern cancer therapies. Targeting fibroblast growth factors (FGFs) and their receptors (FGFRs) is becoming crucial, as their high activity significantly contributes to cancer development and progression by driving cell proliferation and activating signaling pathways that enhance drug resistance. Methods: We investigated the potential of honokiol and FGF ligand trap in blocking the FGF1/FGFR1 axis to counteract drug resistance. Using PEAQ-ITC, we verified direct interaction of honokiol with the FGFR1 kinase domain. We then demonstrated the effect of FGF1/FGFR1 inhibition on taltobulin resistance in cells expressing FGFR1. Finally, we generated drug-resistant clones by prolonged exposure of cells with negligible FGFR levels to taltobulin alone, taltobulin and honokiol, or taltobulin and FGF ligand trap. Results: We demonstrated for the first time a direct interaction of honokiol with the FGFR1 kinase domain, resulting in inhibition of downstream signaling pathways. We revealed that both honokiol and FGF ligand trap prevent FGF1- dependent protection against taltobulin in cancer cells expressing FGFR1. In addition, we showed that cells obtained by long-term exposure to taltobulin are resistant to both taltobulin and other microtubule-targeting drugs, and exhibit elevated levels of FGFR1 and cyclin D. We also found that the presence of FGFligand trap prevents the development of long-term resistance to taltobulin. Conclusion: Our results shed light on how blocking the FGF1/FGFR1 axis by honokiol and FGF ligand trap could help develop more effective cancer therapies, potentially preventing the emergence of drug-resistant relapses. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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45. Mapping and visualization of global research progress on deubiquitinases in ovarian cancer: a bibliometric analysis.
- Author
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Fang Qiu, Yuntong Li, Lile Zhou, Yingli Wu, Yunzhao Wu, Zhilei Fan, Yingying Wang, Dongjun Qin, and Chaoqun Li
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DEUBIQUITINATING enzymes ,BIBLIOMETRICS ,OVARIAN cancer ,BIOCHEMISTRY ,CARCINOGENESIS - Abstract
Background: Ovarian cancer is a highly aggressive malignancy with limited therapeutic options and a poor prognosis. Deubiquitinating enzymes (DUBs) have emerged as critical regulators of protein ubiquitination and proteasomal degradation, influencing various cellular processes relevant to cancer pathogenesis. In this study, the research progress between ovarian cancer and DUBs was mapped and visualized using bibliometrics, and the expression patterns and biological roles of DUBs in ovarian cancer were summarized. Methods: Studies related to DUBs in ovarian cancer were extracted from the Web of Science Core Collection (WoSCC) database. VOSviewer 1.6.20, CiteSpace 6.3.R1, and R4.3.3 were used for bibliometric analysis and visualization. Results: For analysis 243 articles were included in this study. The number of publications on DUBs in ovarian cancer has gradually increased each year. China, the United States, and the United Kingdom are at the center of this field of research. The Johns Hopkins University, Genentech, and Roche Holding are the main research institutions. David Komander, Zhihua Liu, and Richard Roden are the top authors in this field. The top five journals with the largest publication volumes in this field are Biochemical and Biophysical Research Communications, Journal of Biological Chemistry, PLOS One, Nature Communications, and Oncotarget. Keyword burst analysis identified five research areas: "deubiquitinating enzyme," "expression," "activation," "degradation," and "ubiquitin." In addition, we summarized the expression profiles and biological roles of DUBs in ovarian cancer, highlighting their roles in tumor initiation, growth, chemoresistance, and metastasis. Conclusion: An overview of the research progress is provided in this study on DUBs in ovarian cancer over the last three decades. It offers insight into the most cited papers and authors, core journals, and identified new trends. [ABSTRACT FROM AUTHOR]
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- 2024
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46. Analytical validation of the LungLB test: a 4-color fluorescence in-situ hybridization assay for the evaluation of indeterminate pulmonary nodules.
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Lutman, Michelle L., Gramajo-Leventon, Daniel, Tahvilian, Shahram, Baden, Lara, Gilbert, Courtney L., Trejo, Michael, Vail, Eric, Donovan, Michael J., Katchman, Benjamin A., and Pagano, Paul C.
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FLUORESCENCE in situ hybridization ,LUNG cancer ,EARLY detection of cancer ,CARCINOGENESIS ,PROGNOSTIC tests - Abstract
Background: Evaluation of indeterminate pulmonary nodules (IPNs) often creates a diagnostic conundrum which may delay the early detection of lung cancer. Rare circulating genetically abnormal cells (CGAC) have previously demonstrated utility as a biomarker for discriminating benign from malignant small IPNs in the LungLB assay. CGAC are identified using a unique 4-color fluorescence in-situ hybridization (FISH) assay and are thought to reflect early cell-based events in lung cancer pathogenesis and the anti-tumor immune response. LungLB is a prognostic tool that combines the CGAC biomarker and clinical features to aid in IPN evaluation by improving the stratification of patient risk of malignancy. Methods: Herein we describe the analytical performance of the LungLB blood test. Analytical validation was performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines with adaptations for rare cell-based assays. Multiple operators, reagent lots, and assay runs were tested to examine accuracy, precision, reproducibility, and interfering factors. Results: The FISH probes used in the LungLB assay demonstrate 100% sensitivity and specificity for their intended chromosomal loci (3q29, 3p22.1, 10q22.3 and 10cen). LungLB demonstrates analytical sensitivity of 10 CGAC per 10,000 lymphocytes analyzed, 100% analytical specificity, and high linearity (R
2 = 0.9971). Within run measurements across 100 samples demonstrated 96% reproducibility. Interfering factors normally found in blood (lipemia, biotin) and exposure to adverse temperatures (-20ºC or 37ºC) did not interfere with results. Sample stability was validated to 96 hours. Conclusion: The analytical performance of LungLB in this validation study successfully demonstrates it is robust and suitable for everyday clinical use. [ABSTRACT FROM AUTHOR]- Published
- 2024
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47. Cancer cell stiffening via CoQ10 and UBIAD1 regulates ECM signaling and ferroptosis in breast cancer.
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Tosi, Giovanni, Paoli, Alessandro, Zuccolotto, Gaia, Turco, Emilia, Simonato, Manuela, Tosoni, Daniela, Tucci, Francesco, Lugato, Pietro, Giomo, Monica, Elvassore, Nicola, Rosato, Antonio, Cogo, Paola, Pece, Salvatore, and Santoro, Massimo M.
- Subjects
BREAST cancer ,CELL membranes ,EXTRACELLULAR matrix ,CANCER invasiveness ,CARCINOGENESIS - Abstract
CoQ
10 (Coenzyme Q10 ) is an essential fat-soluble metabolite that plays a key role in cellular metabolism. A less-known function of CoQ10 is whether it may act as a plasma membrane-stabilizing agent and whether this property can affect cancer development and progression. Here, we show that CoQ10 and its biosynthetic enzyme UBIAD1 play a critical role in plasmamembrane mechanical properties that are of interest for breast cancer (BC) progression and treatment. CoQ10 and UBIAD1 increase membrane fluidity leading to increased cell stiffness in BC. Furthermore, CoQ10 and UBIAD1 states impair ECM (extracellular matrix)-mediated oncogenic signaling and reduce ferroptosis resistance in BC settings. Analyses on human patients and mouse models reveal that UBIAD1 loss is associated with BC development and progression and UBIAD1 expression in BC limits CTCs (circulating tumor cells) survival and lung metastasis formation. Overall, this study reveals that CoQ10 and UBIAD1 can be further investigated to develop therapeutic interventions to treat BC patients with poor prognosis. The roles of CoQ10 and producing enzymes in cancer have not been well explored. Here, the authors identify that the CoQ10-biosynthetic enzyme UBIAD1 is a tumor suppressor in breast cancer. CoQ10 induced alterations in plasma membrane mechanical properties lead to increased cellular stiffness and impaired tumorigenic signaling, as well as enhanced sensitivity to ferroptosis. [ABSTRACT FROM AUTHOR]- Published
- 2024
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48. Privileged Scaffold Hybridization in the Design of Carbonic Anhydrase Inhibitors.
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Secci, Daniela, Sanna, Erica, Distinto, Simona, Onali, Alessia, Lupia, Antonio, Demuru, Laura, Atzeni, Giulia, Meleddu, Rita, Cottiglia, Filippo, Angeli, Andrea, Supuran, Claudiu T., and Maccioni, Elias
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CARBONIC anhydrase inhibitors , *ISATIN , *MOLECULAR docking , *CARCINOGENESIS , *5G networks - Abstract
Human Carbonic Anhydrases (hCA) are enzymes that contribute to cancer's development and progression. Isoforms IX and XII have been identified as potential anticancer targets, and, more specifically, hCA IX is overexpressed in hypoxic tumor cells, where it plays an important role in reprogramming the metabolism. With the aim to find new inhibitors towards IX and XII isoforms, the hybridization of the privileged scaffolds isatin, dihydrothiazole, and benzenesulfonamide was investigated in order to explore how it may affect the activity and selectivity of the hCA isoforms. In this respect, a series of isatin thiazolidinone hybrids have been designed and synthesized and their biological activity and selectivity on hCA I, hCA II, hCA IX, and hCA XII explored. The new compounds exhibited promising inhibitory activity results on isoforms IX and XII in the nanomolar range, which has highlighted the importance of substituents in the isatin ring and in position 3 and 5 of thiazolidinone. In particular, compound 5g was the most active toward hCA IX, while 5f was the most potent inhibitor of hCA XII within the series. When both potency and selectivity were considered, compound 5f appeared as one of the most promising. Additionally, our investigations were supported by molecular docking experiments, which have highlighted the putative binding poses of the most promising compound. [ABSTRACT FROM AUTHOR]
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- 2024
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49. Unique Gene Expression Profiles within South Africa Are Associated with Varied Chemotherapeutic Responses in Conventional Osteosarcoma.
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Mthethwa, Phakamani G., Arumugam, Thilona, Ramsuran, Veron, Gokul, Anmol, Rodseth, Reitze, and Marais, Leonard
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OSTEOSARCOMA , *RESEARCH funding , *OSTEOBLASTS , *MULTIPLE regression analysis , *POLYMERASE chain reaction , *GLYCOPROTEINS , *QUANTITATIVE research , *DESCRIPTIVE statistics , *CANCER chemotherapy , *MESSENGER RNA , *ODDS ratio , *LONGITUDINAL method , *GENE expression , *DNA methylation , *GENE expression profiling , *CARTILAGE cells , *DNA repair , *HISTOLOGICAL techniques , *CARCINOGENESIS , *CONFIDENCE intervals , *DATA analysis software , *COMPARATIVE studies , *IMMUNITY - Abstract
Simple Summary: This study aimed to determine the gene expression profiles associated with chemotherapeutic responses in conventional osteosarcomas (COS) within South Africa. We observed a significant downregulation in the ATP binding cassette subfamily C members (ABCC3 and ABCB1-p-glycoprotein), excision repair cross-complimenting group 1 (ERCC 1), replication factor C subunit 1 (RFC1), and tumour protein 53 (p53) genes in the COS tumours compared to the healthy donors. Furthermore, an upregulated ERCC1 gene expression level predicted a poor chemotherapeutic response. Additionally, the predictors of COS chemotherapeutic response comprised age, chondroblastic and osteoblastic histological subtypes, and ABCC3, ERCC1, and RFC1 gene expression. Background: We determined the predictive gene expression profiles associated with chemo-response in conventional osteosarcomas (COS) within South Africa. Materials and methods: In 28 patients, we performed an RNA extraction, cDNA synthesis, and quantitative analysis using the RT-PCR 2−∆∆CT method to determine the fold change in gene expression alongside GAPDH (housekeeping gene). Results: We observed a significant downregulation in the mRNA expression profiles of ABCB1-p-glycoprotein (p = 0.0007), ABCC3 (p = 0.002), ERCC1 (p = 0.007), p-53 (p = 0.007), and RFC1 (p = 0.003) in the COS patients compared to the healthy donors. Furthermore, ABCB1-p-glycoprotein (p = 0.008) and ABCC3 (p = 0.020) exhibited a significant downregulation in the COS tumour tissues when compared to the healthy donors. In our univariate logistic regression, the predictors of chemotherapeutic response comprised ERCC1 [restricted cubic spline (RCS) knot: OR −0.27; CI −0.504 to −0.032; p = 0.036]; osteoblastic subtype [OR −0.36; CI −0.652 to −0.092; p = 0.026); fibroblastic subtype [OR 0.91; CI 0.569 to 1.248; p < 0.001]; and mixed subtype [OR 0.53; CI 0.232 to 0.032; p = 0.032]. In our multivariable logistic regression, the significant predictors of chemotherapeutic response comprised age [RCS knot: OR −2.5; CI −3.616 to −1.378; p = 0.022]; ABCC3 [RCS knot: OR 0.67; CI 0.407 to 0.936, p = 0.016]; ERCC1 [RCS knot: OR 0.57; CI 0.235 to 0.901; p = 0.044]; RFC1 [RCS knot: OR −1.04; CI −1.592 to −0.487; p = 0.035]; chondroblastic subtype [OR −0.83; CI −1.106 to −0.520; p = 0.012]; and osteoblastic subtype [OR −1.28; CI −1.664 to −0.901; p = 0.007]. Conclusions: In this South African cohort, we observed the unique gene expression profiles of osteosarcoma tumourigenesis and chemotherapeutic responses. These may serve as prognostication and therapeutic targets. Larger-scale research is needed on the African continent. [ABSTRACT FROM AUTHOR]
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- 2024
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50. The Potential of Human Pulmonary Mesenchymal Stem Cells as Vectors for Radiosensitizing Metallic Nanoparticles: An In Vitro Study †.
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Arcambal, Angélique, Septembre-Malaterre, Axelle, Pesnel, Sabrina, Morel, Anne-Laure, Gasque, Philippe, Begue, Mickael, and Slama, Youssef
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TREATMENT of lung tumors , *IRON oxide nanoparticles , *OXIDATION-reduction reaction , *IN vitro studies , *VASCULAR endothelial growth factors , *CHEMOKINES , *RESEARCH funding , *PHENOMENOLOGICAL biology , *MESENCHYMAL stem cells , *ENZYME-linked immunosorbent assay , *PLATELET-derived growth factor , *GENETIC engineering , *TUMOR markers , *REVERSE transcriptase polymerase chain reaction , *BIOCHEMISTRY , *OXIDATIVE stress , *RADIATION-sensitizing agents , *GENE expression , *CELL lines , *CELL death , *LUNG tumors , *TUMORS , *CARCINOGENESIS , *STAINS & staining (Microscopy) , *CELL survival , *CYTOKINES - Abstract
Simple Summary: Currently, delivering nanoparticles to hard-to-reach tumor sites remains a challenge in nanomedicine. Mesenchymal stem cells are an innovative strategy for targeting tumors, and genetic engineering could enable them to release antitumor agents and/or nanomaterials at tumor sites. Therefore, combining radiosensitizing agents with mesenchymal stem cells represents a promising strategy in the fight against cancer. Still, evaluating whether nanoparticles can modulate mesenchymal stem cells' behavior is essential. This study assessed the impact of new Fe3O4@Au nanoparticles on human pulmonary mesenchymal stem cells to determine whether they can be used as carriers for radiosensitizer agents to cancer sites. This study focused on the markers related to cell death, redox and proinflammatory status, and tumorigenesis. Background/Objectives: Metallic nanoparticles (NPs) exhibit interesting radiosensitizing effects, and finding a way to accurately deliver them appears to be crucial. Due to their tumor tropism, mesenchymal stem cells (MSCs) represent a strategic approach. Therefore, we aimed to evaluate the impact of core–shell Fe3O4@Au NPs on the functionality of human pulmonary MSCs (HPMSCs). Methods/Results: The results showed that 100 µg/mL Fe3O4@Au NPs, accumulated in HPMSCs (revealed by Prussian blue staining), did not alter cell viability as assessed by cell counting, MTT, and LDH assays. However, caspase 9 and Bcl2 gene expression, evaluated by RT-qPCR, was regulated 72 h after exposure to the NPs. Moreover, the NPs also decreased proinflammatory cytokine/chemokine secretions, except for CXCL8 (ELISA). These modulations were associated with the downregulation of AMPK gene expression at 24 h. In contrast, the NPs did not modulate VEGF, PI3K, or PDGF gene expression. Nevertheless, a decrease in VEGF secretion was observed after 24 h of exposure to the NPs. Interestingly, the Fe3O4@Au NPs did not modulate Nrf2 gene expression, but they did regulate the expression of the genes encoding Nox4 and HMOX-1. Additionally, the NPs increased ROS production, suggesting a redox imbalance. Conclusions: Finally, the Fe3O4@Au NPs did not affect the HPMSCs' viability or proangiogenic/tumorigenic markers. These findings are encouraging for investigating the effects of Fe3O4@Au NPs delivered by HPMSCs to tumor sites in combination with radiation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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- View/download PDF
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