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A zenodo library associated to the publication : Improving crosstalk assessment in multicolor Single Molecule Localization Microscopy&nbsp

Melanophryniscus macrogranulosus The advertisement call of Melanophryniscus macrogranulosus from Garapiá is composed of two segments of total duration of 21.3 seconds (SD = 5.8) (Figure 3C). Part A is a series of 5–14.3 notes of one pulse (Mean = 6.5; SD = 4.23), each lasting on average 0.03 sec (SD = 0.04) and separated by long time intervals ranging from 0.31–0.53 seconds (Mean = 0.34; SD = 0.08). It is followed by part B, a long train of unmodulated pulses lasting from 10.3–23.5 sec (Mean= 18.6; SD = 4.9), emitted at a rate of 35.3 pulses per second (SD = 1.05), with short time intervals. The call presented a similar peak frequency in both segments being in average 2.4 kHz (SD = 0.3). The population from Morro da Gruta, which is the type locality, also shows a call composed of two segments A and B, with a total duration of 17.4 sec on average (SD = 1.9) (Figure 3D). Part A is also a series of notes (Mean = 4; SD = 1.5) composed of one pulse, each lasting on average 0.023 sec (SD = 0.09) and separated by long time intervals ranging from 0.28–0.53 seconds (Mean = 0.35; SD = 0.09). It is followed by part B, a long train of unmodulated pulses lasting from 13.3–16.4sec (Mean= 14.8; SD = 1.13), emitted at a rate of 34.9 pulses per second (SD = 1.63), with short time intervals. The call presented a similar peak frequency in both segments being on average 2.3 kHz (SD = 0.16). Values separated by population are shown in Table 1 and by species in Table 4., Published as part of Caorsi, Valentina, Bordignon, Debora Wolff, Márquez, Rafael & Martins, Márcio Borges-, 2020, Advertisement call of two threatened red-bellied-toads Melanophryniscus cambaraensis and M. macrogranulosus (Anura: Bufonidae), from the Atlantic Rainforest, southern Brazil, pp. 206-220 in Zootaxa 4894 (2) on page 211, DOI: 10.11646/zootaxa.4894.2.2, http://zenodo.org/record/4315653

Melanophryniscus cambaraensis The advertisement call of Melanophryniscus cambaraensis from FLONA SFP has an average duration of 18.8 seconds (SD = 6.51) and it is composed of segments A and B (Figure 3 A–B). Part A is composed of 3–11 single modulated notes, each one composed of one pulse (of 0.02 seconds each), separated by long time intervals of 0.38 seconds (SD = 0.052). Call part B is an extremely long train of unmodulated pulses emitted at a rate of 35.8 pulses per second (SD = 3.1), with short time intervals (Mean = 0.02; SD = 0.002), and lasting from 9–32.2 seconds (Mean = 16.6; SD = 6.8) (Table 1). Peak frequency of both parts is quite similar being on average 2.2 kHz in part A (SD = 0.16) and 2.2 kHz in part B (SD = 0.17) (Table 1)., Published as part of Caorsi, Valentina, Bordignon, Debora Wolff, Márquez, Rafael & Martins, Márcio Borges-, 2020, Advertisement call of two threatened red-bellied-toads Melanophryniscus cambaraensis and M. macrogranulosus (Anura: Bufonidae), from the Atlantic Rainforest, southern Brazil, pp. 206-220 in Zootaxa 4894 (2) on page 210, DOI: 10.11646/zootaxa.4894.2.2, http://zenodo.org/record/4315653

International audience; The configurations associated with reversible and irreversible adsorption of hydrogen on MgO escape consensus. Here, we report the dissociation of H2 on MgO nanocubes, which was examined by combining Fourier transform infrared spectroscopy and density functional theory (DFT)-based simulations. We found that the use of ultrahigh vacuum is essential for identifying the very first adsorption stages. Hydrogen pressure was varied from 10–8 mbar to near ambient, resulting in IR spectra of richer complexity than in current state of the art. Models with oxygen at regular corners (O3C) and Mg at inverse corners (MgIC) were identified to be the most reactive and to split H2 irreversibly already in the lowest pressure regime (PH2 < 10–3 mbar). The continuous increase in intensity of the corresponding IR bands (3712/1140 cm–1) in the intermediate range of pressures (10–3–1 mbar), along with the appearance of bands at 3605/1225 cm–1, was demonstrated to stem from cooperative adsorption mechanisms, which could be therefore considered as the main origin of irreversible hydrogen adsorption. At PH2 > 1 mbar, fully reversible adsorption was shown to occur at O4C (either on mono- or diatomic steps) and Mg3C sites. Another OH/MgH couple (3697/1030 cm–1) that became reinforced at high PH2 but remained stable upon pumping was correlated to O3C and MgIC in multiatomic steps. The difference in adsorption and desorption sequences confirmed the proposed cooperative adsorption of H2 molecules. Our study provides new insights into the mechanisms that can be beneficial for understanding the chemistry of H2 and other hydrogen-containing molecules, such as CH4, on oxide surfaces, but also for the advancement of hydrogen-storage technologies.

In this study, we aimed to identify the myosin motor proteins that control trafficking at the Golgi complex. In addition to the known Golgi-associated myosins MYO6, MYO18A and MYH9 (myosin IIA), we identified MYO1C as a novel player at the Golgi in a human cell line. We demonstrate that depletion of MYO1C induces Golgi complex fragmentation and decompaction. MYO1C accumulates at dynamic structures around the Golgi complex that colocalize with Golgi-associated actin dots. MYO1C depletion leads to loss of cellular F-actin, and Golgi complex decompaction is also observed after inhibition or loss of the actin-related protein 2/3 complex, Arp2/3 (also known as ARPC). We show that the functional consequence of MYO1C depletion is a delay in the arrival of incoming transport carriers, both from the anterograde and retrograde routes. We propose that MYO1C stabilizes actin at the Golgi complex, facilitating the arrival of incoming transport carriers at the Golgi.This article has an associated First Person interview with the first author of the paper. Fil: Capmany, Anahi. Institute Curie; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Inmunologia; Argentina Fil: Yoshimura, Azumi. Institute Curie; Francia Fil: Kerdous, Rachid. Institute Curie; Francia Fil: Caorsi, Valentina. Abbelight; Francia Fil: Lescure, Aurianne. Institute Curie; Francia Fil: Nery, Elaine Del. Institute Curie; Francia Fil: Coudrier, Evelyne. Institute Curie; Francia Fil: Goud, Bruno. Institute Curie; Francia Fil: Schauer, Kristine. Institute Curie; Francia

Distúrbios antropogênicos têm sido apontados como a principal causa da perda da biodiversidade mundial. Dentre eles, a poluição sonora é uma potencial, porém subestimada, ameaça, prevista para aumentar nos próximos anos, juntamente com a expansão urbana. Ruídos antropogênicos podem ter efeitos negativos, especialmente em espécies que dependem da comunicação acústica. Entretanto, a poluição sonora pode afetar não só a comunicação, mas as funções auditivas de maneira geral. Desta forma, ruído pode agir como um estressor geral, influenciando processos vitais, desde a integridade do DNA, a processos fisiológicos ou comportamentais ou até se estender a populações e comunidades. O objetivo desta tese é contribuir com o conhecimento sobre o efeito do ruído antropogênico em animais, estudando sistemas não auditivos e auditivos afetados por ele. O primeiro objetivo teve foco no distúrbio do ruído gerado em cidades no comportamento de sono em aves (i). Os outros três objetivos da tese estão relacionados com a comunicação e o sistema auditivo de anfíbios anuros. Mais especificamente, testamos (ii) o efeito do ruído antropogênico audível (tráfego) e (iii) sísmico (tráfego e eólicos) no canto de algumas espécies; e por último, (iv) apresentamos uma revisão da literatura existente sobre a extensão dos feitos do ruído antropogênico em anuros. Os resultados deste trabalho mostraram que tanto o ruído audível, como o sísmico proveniente de diferentes fontes de atividade humana tem um efeito sobre o comportamento dos animais e que diferentes espécies respondem de distintas maneiras frente a este estressor. Fica claro que a poluição sonora é uma fonte importante de distúrbio em animais, com efeitos adversos e que deve, então, ser levada em conta como possível fator de impacto para as espécies e incluída em futuros estudos e legislações, a fim de controlar seus efeitos na biodiversidade. Anthropogenic disturbance has been pointed as the major cause of the world ́s biodiversity crisis. Among them, noise pollution is a potential underestimated threat, projected to increase in the next decades accompanying urban expansion. Rising levels of noise pollution may result in negative impacts on species, specially the ones depending on acoustic communication. However, compromise hearing affects more than acoustical communication. It has been shown to influence from DNA integrity and genes, to physiological systems, behavioral ecology and community ecology. The aim of this thesis is to contribute with knowledge about the effect of anthropogenic noise in animals, studying non-auditory and auditory systems affected by it. The first goal focused on the effect of urban noise in sleep behavior in birds (i). The other three goals were related to the communication and auditory system of anuran amphibians. More specifically, we tested (ii) the effect of audible (traffic) and (iii) seismic (traffic and wind) anthropogenic noise in the calling behavior of some species; finally, (iv) we reviewed the existing literature on the extent of anthropogenic noise in anurans. The results of this work showed that both audible and seismic anthropogenic noise have an effect on the behavior of the animals, but with species responding in different ways to this stressor. It is clear that noise pollution is an important source of disturbance in animals, with adverse effects and, then, it must be taken into account as a possible impact factor for species and be included in future studies and legislation in order to control it effects on biodiversity.

Anthropogenic disturbance has been pointed to as one of the major causes of the world´s biodiversity crisis. Among them, noise pollution is a potential underestimated threat, projected to increase in the next decades accompanying urban expansion. Rising levels of noise pollution may result in negative impacts on species highly dependent on acoustic communication. Amphibians have long served as model organisms for investigating animal acoustic communication because their reproduction depends on transmitting and receiving acoustic signals. A few studies have investigated the effects of anthropogenic noise on anurans, but there is still limited knowledge on how it affects them. In this study, we test the effect of two intensities of traffic noise on calling males of two Neotropical treefrogs species. We expect to record more changes in call parameters, to avoid masking effect, at higher intensity noise treatments, and in the species with higher call/noise frequency overlap. We performed a set of field playback experiments exposing male frogs to road noise at two different intensities (65dB and 75dB). Focal species are Boana bischoffi (high call/noise frequency overlap) and B. leptolineata (low call/noise frequency overlap). Both species changed acoustic parameters during or after the exposure to traffic noise. Advertisement call rate of B. bischoffi decreased during road noise, and dominant frequency decreased over time. Call length of B. leptolineata increased or decreased, depending on the order of noise intensity. We also observed spatial displacement in both species, which moved away from the noise source. Our results provide evidence that traffic noise affects anuran calling behavior, and noise intensity is an important factor affecting how species respond.

Melanophryniscus macrogranulosus Braun, 1973 foi descrita com base em nove exemplares procedentes do Morro da Gruta, Dom Pedro de Alcântara, Rio Grande do Sul. Sua distribuição foi ampliada com um registro para Barra do Ouro, Maquiné, RS. Melanophryniscus cambaraensis Braun & Braun, 1979 foi descrita com base em exemplares provenientes da Fortaleza dos Aparados, Cambará do Sul, RS, sendo a primeira espécie do gênero descrita com coloração dorsal verde. Posteriormente, foi registrada na Floresta Nacional de São Francisco de Paula (FLONA), RS. Ambas espécies são consideradas ameaçadas de extinção, devido a destruição e fragmentação de seu habitat. As duas espécies foram originalmente diagnosticadas pelo “diferente tipo de ambiente” que habitam e por sua coloração. Contudo, não é possível diferenciar as populações atualmente conhecidas por estes parâmetros, existindo um problema taxonômico. O objetivo do nosso trabalho foi esclarecer a identidade taxonômica delas, através de um estudo morfológico, incluindo a variação individual de padrão de coloração, genético e bioacústico. As atividades de campo foram desenvolvidas no Rio Grande do Sul no período entre 2012 e 2013. A fotoidentificação visual e automatizada (software) foram testadas na população da FLONA de São Francisco de Paula usando a amputação de falanges como método de controle. A maior acurácia foi registrada com a fotoidentificação visual (99.4%), seguido da amputação de falanges (95.3%) e, por último, com a utilização do software (90.9%). Quanto ao estudo taxonômico, as análises de morfologia e genética não evidenciaram a existência de caracteres diagnósticos para as duas espécies referidas. O estudo da morfologia desta espécie mostrou que os calos nupciais em machos de M. macrogranulosus estão presentes nos dedos I (polegar), II e III. As observações feitas também identificaram o primeiro registro destas estruturas dérmicas em fêmeas, dentro do gênero Melanophryniscus. O estudo bioacústico da espécie, mostrou que o canto de anúncio dela se inicia com o canto do tipo A, composto por pulsos únicos e é sempre seguida do canto do tipo B, um trinado extremamente longo. Foi encontrada uma alta variação em todos os níveis estudados: intra e inter-individual e populacional, não sendo possível a separação clara de grupos. Os aspectos morfológicos, genéticos e bioacústicos estudados não corroboraram o status taxonômico atual das duas espécies, reconhecidas atualmente como válidas. Portanto, M. cambaraensis Braun & Braun, 1979 foi considerado um sinônimo Junior de M. macrogranulosus. Utilizando todos os dados adquiridos ao longo do trabalho e seguindo os critérios da lista vermelha da IUCN, a espécie ficou enquadrada na categoria “Em Perigo”.

Anthropogenic disturbance is a major cause of the biodiversity crisis. Nevertheless, the role of anthropogenic substrate vibrations in disrupting animal behavior is poorly understood. Amphibians comprise the terrestrial vertebrates most sensitive to vibrations, and since communication is crucial to their survival and reproduction, they are a suitable model for investigating this timely subject. Playback tests were used to assess the effects of substrate vibrations produced by two sources of anthropogenic activity– road traffic and wind turbines– on the calling activity of a naïve population of terrestrial toads. In their natural habitat, a buried tactile sound transducer was used to emit simulated traffic and wind turbine vibrations, and changes in the toads’ acoustic responses were analyzed by measuring parameters important for reproductive success: call rate, call duration and dominant frequency. Our results showed a significant call rate reduction by males of Alytes obstetricans in response to both seismic sources, whereas other parameters remained stable. Since females of several species prefer males with higher call rates, our results suggest that anthropogenically derived substrate-borne vibrations could reduce individual reproductive success. Our study demonstrates a clear negative effect of anthropogenic vibrations on anuran communication, and the urgent need for further investigation in this area., This study was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (VG: #380320/2018-5; CB. 401076/2014-8; 312968/2013-2) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (www.capes.gov.br/CAPES VC.3471301; 88881.135522/2016-01). Partial funding for this project was generously provided by PPG-BAN UFRGS in VZC and MBM lab; by Daniel Hurley for support of student research in the PMN lab and by Ministerio de Economía, Industria y Competitividad (CGL2017-88764-R, MINECO/AEI/FEDER, Spain) in the DL lab.