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Five experiments were conducted to evaluate the lysine (Lys) requirements of lactating sows. All diets were formulated to be isocaloric 3.46 Mcal ME/kg and met or exceeded National Research Council recommendations. In all studies, sow feed intake, body weight loss/gain, subsequent reproduction, and litter growth rate (LGR) were evaluated. The data were analyzed as randomized complete block design using generalized linear model in SAS with parity as a block. Two hundred and sixty-four primiparous sows (PIC Camborough 22) were randomly allotted to one of five lactation treatments (total Lys of 0.95%, 1.05%, 1.15%, 1.25%, and 1.35%) in Exp. 1 from August 2005 through October 2005. As daily total dietary Lys intake increased from 52.10 to 77.53 g, piglet ADG and daily litter gain linearly improved (P < 0.01). From February 2007 through April 2007, 336 multiparous sows (parity 4 and older, PIC Camborough 29) were randomly allotted to one of five lactation treatments (total Lys 0.85%, 0.95%, 1.05%, 1.15%, or 1.25%) in Exp. 2. As dietary total Lys increased from 0.85% to 1.25% of the diet, there were no significant differences in litter performance, such as ADG, daily litter gain, and the number of pigs weaned. Experiment 3 was conducted from October 2008 through January 2009. Two hundred and seventy-nine primiparous gilts (PIC Camborough 29) were randomly allotted to one of five lactation treatments (total Lys 1.14%, 1.25%, 1.35%, 1.46%, and 1.57%). Actual total Lys intakes increased from 56.74 to 77.12 g/d. Feeding total dietary Lys quadratically decreased (P < 0.01) weaning-to-estrus interval and increased percentage bred by 10 d (P = 0.02). In Exp. 4, 200 sows (parity 4 and older, PIC Camborough 29) were randomly allotted to one of five treatments (0.85%, 0.95%, 1.05%, 1.15%, or 1.25% total Lys) from January 2008 through March 2008. As dietary total Lys increased from 42.40 to 66.15 g/d, sow body weight and LGRs were not influenced by dietary total Lys intakes. In Exp. 5, 324 parity 3 sows (PIC Camborough 29) were randomly allotted to one of five treatments (0.77%, 0.92%, 1.08%, 1.23%, and 1.38% total Lys) from August 2009 through October 2009. As daily dietary total Lys intake increased from 39.44 to 67.32 g, the percentage of sows bred by 10 d increased (P = 0.02), as well as the LGR. A broken-line quadratic regression analysis demonstrated that the total Lys requirement for LGR for parity 1 females is calculated as 72.68 − [6.04 × (3.55 − LGR)] and for parity 3+ females as 92.03 − [11.9 × (4.24 − LGR)].

Background The use of feed grade amino acids can reduce the cost of lactation feed. With changing genetics, increasing feed costs, and higher number of pigs weaned with heavier wean weights further evaluation of higher inclusion levels of feed-grade amino acid in lactation diets than previously published is warranted. Two experiments (Exp.) were conducted to determine the optimal inclusion level of L-lysine HCl to be included in swine lactation diets while digestible lysine levels remain constant across dietary treatments and allowing feed grade amino acids to be added to the diet to maintain dietary ratios relative to lysine to maximize litter growth rate and sow reproductive performance. Furthermore, the studies were to evaluate minimal amino acid ratios relative to lysine that allows for optimal litter growth rate and sow reproductive performance. Results Exp. 1: Increasing L-lysine HCl resulted in similar gilt feed intake, litter, and reproductive performance. Average litter gain from birth to weaning was 2.51, 2.49, 2.59, 2.43, and 2.65 kg/d when gilts were fed 0.00, 0.075, 0.150, 0.225, and 0.30% L-lysine HCl, respectively. Exp. 2: The average litter gain from birth to weaning was 2.68, 2.73, 2.67, 2.70, and 2.64 kg/d (P

SUMMARY The objective of this study was to investigate the impact of long-term inclusion of the trace elements zinc, copper, and manganese chelated with the hydroxy analogue of methionine on layer performance, eggshell quality, tibia breaking strength, and immune response. A total of 216 Hy-Line W-36 laying hens were allocated to 6 treatments with 36 pens/treatment and 1 hen/cageusingarandomizedcompleteblockdesign.Therewere6treatmentswithsupplemental Zn-Cu-Mn, as follows: T1 0-0-0 ppm, T2 20-5-20 ppm as sulfates, T3 20-5-20 ppm as chelates, T4 40-10-40 ppm as sulfates, T5 40-10-40 ppm as chelates, and T6 80-10-80 as sulfates. The feeding of dietary treatments started at wk 24 pullet age and measurements of the key parameters were made from wk 44 to 80. Overall results (one-way ANOVA) indicated a significant treatment effect (P 0.05) response to T1 and T6 but different (P < 0.05) from T2. Factorial analysis of the data showed an improvement in shell breaking strength (P < 0.05 at wk 68), shell thickness (P = 0.08 at wk 68; P = 0.03 at wk 74), tibia breaking strength (P = 0.07) and antibody (Ab) titers to SRBCs (P < 0.05 at wk 63; 1 wk after secondary antigen challenge) in hens fed chelated trace mineral when compared to inorganic trace minerals. In summary, feeding laying hens’ diets containing supplemental chelated trace minerals showed improvements in eggshell thickness and immune response when compared to inorganic salts at specific time points measured during the latter part of the laying cycle.

An experiment was conducted to characterize a gut health challenge model consisting of a diet containing rye, wheat, and feather meal and a mild mixed-species Eimeria challenge, and to evaluate the effect of carbohydrase and protease on growth performance and gut health of young broilers. The study included 4 treatments: negative control, carbohydrase alone, protease alone, and combination of carbohydrase and protease. Each test diet was fed to 18 battery pens of broilers with 8 male birds per pen from 0 to 22 d of age. Carbohydrase improved body weight, feed intake, and feed conversion ratio (FCR) on d 7, 14, and 21(P < 0.01). Protease increased body weight on d 7 and 21 and improved 0 to 7 d FCR (P < 0.05). More lymphocyte infiltration was observed in small intestine mucosa of negative control birds on d 8, carbohydrase supplementation lessened this. Both carbohydrase and protease reduced digesta viscosity on d 22 with the carbohydrase effect being the greater of the two, and the combination effect was not different from the carbohydrase effect alone (P < 0.01). Ileal Clostridium perfringens of 15-day-old broilers was decreased by carbohydrase, a further reduction was achieved by combining carbohydrase with protease (P = 0.01). Liver vitamin E concentration on d 15 (P < 0.01) and 22 (P = 0.02) was increased by carbohydrase, and the carbohydrase effect was greater in the presence of protease on d 22 (P = 0.04). Plasma α-1-acid glycoprotein level and liver Zn and Cu concentrations of broilers were reduced by carbohydrase on d 15 (P < 0.01). Broilers fed carbohydrase had higher levels of plasma zeaxanthin on d 22 and higher levels of plasma lutein on d 15 and 22 (P < 0.01). In summary, a rye wheat based diet containing feather meal when fed to broilers in addition to a mild Eimeria challenge induced subclinical enteritis characterized by digestion inefficiency, dysbacteriosis, inflammation, and gut barrier failure; carbohydrase and protease could be effective tools to improve growth performance and gut health of broilers suffering from this type of subclinical enteritis.

Multidisciplinary Design Optimization (MDO) studies show the Strut/Truss Braced Wing (SBW/TBW) concept has the potential to save a significant amount of fuel over conventional designs. For the SBW/TBW concept to achieve these reductions, the interference drag at the wing strut juncture must be small compared to other drag sources. Computational Fluid Dynamics (CFD) studies have indicated that the interference drag is small enough to be manageable. However, the RANS formulation and turbulence models used in these studies have not been validated for high Reynolds number transonic junction flows. This study assesses turbulence models by comparing flow separation characteristics obtained from experiment and CFD. The test model used is a NACA 0012 wing of aspect ratio 2 at Mach number of 0.76 and a Reynolds number of 6 million with varying angle of attack. The CFD study involved an 18.8 million cell structured grid of the wind tunnel test section using the ANSYS Fluent 12.0 solver. The k-ω SST turbulence model was the main turbulence model employed. Experiments were conducted in a high Reynolds number transonic Ludwieg tunnel. The wing was tested at different Mach numbers and inlet conditions to account for some of the experimental variations. Porous walls eliminate shock reflection across the tunnel. Surface oil flow visualization is used to indicate the interference flow patterns. The assessment shows CFD overpredicts separation and therefore interference drag, likely due to deficiencies in the turbulence model. Nomenclature

SUMMARY The microbial community of the gastrointestinal system has an enormous impact on the vertebrate host. The relationship begins at birth or hatch and evolves to a stable ecosystem in which diverse, and unique, niches are created and inhabited by microorganisms. These microbial populations tend to be similar within a host species (and even across host species), but each system is a unique construct resulting from its individual history of mutual influence. The development of the system, both microbial and host, begins in the perinatal animal. The timing of this developmental process is suggestive of imprinting, the process of epigenetic evolution of somatic stem cells. (Imprinted changes are thought not to involve the germ line, i.e., are not inherited by the next generation of the host animal, but are genetic changes that can be passed on to the daughter cells of the imprinted proliferative stem cell.) This review briefly discusses the development of the gastrointestinal system, including both the microbiota and its perinatal host. The effects of the microflora on enteric and immune cells are described. Effects of attempts to restrict contact between the mucosal immune system and the microbiota are addressed, along with further data that would be required to demonstrate that the effects of the gut microbiota on mucosal immune development are restricted to an ontogenetic window. Finally, the consequences of a failure to achieve a relationship of mutual tolerance between the microbiota and the host and some mechanisms to facilitate this process are discussed.

A total of 1,200 LinNan Chinese color-feathered chicks were used to study the effects of methionine source [DL-2-hydroxy-4-methylthio-butanoic acid (HMTBa) or DL-methionine (DLM)] and dietary crude protein (CP) level on growth performance, carcass traits, and whole-body nitrogen and fat retention. The trial was designed as a 2x2 factorial arrangement, including two CP levels (adequate and low) and two methionine sources (HMTBa and DL-methionine). Diets were formulated for three phases, starter (0-21 d), grower (21-42 d), and finisher (42-63 d). Chicks fed HMTBa had higher daily gain and improved feed efficiency than DLM during the grower phase (p 0.05). Chicks fed low-CP diets grew slower, used feed less efficiently during the grower, finisher phase and overall. On d 42, regardless of dietary CP levels, birds fed HMTBa had higher carcass weights, breast and thigh weights than DLM-fed birds (p

Mintrex Zn, Mintrex Cu, and Mintrex Mn organic trace minerals contain 16% Zn, 15% Cu, and 13% Mn with 80, 78, and 76% 2-hydroxy-4-(methylthio)butanoic acid (HMTBA) by weight as the organic ligand, respectively. Our objective was to determine if HMTBA from Mintrex was fully available as a Met source. In experiment 1, thirty-six broilers (7 to 10 d old) were orally gavaged with methyl-(14)C-labeled HMTBA, either as free HMTBA (Alimet feed supplement) or Zn bis(-2-hydroxy-4-methylthiobutyrate) (Mintrex Zn). Radiolabel incorporation from either source into protein was measured as a marker of bioavailable Met activity. Results demonstrated that the HMTBA from Mintrex Zn was equally available as free HMTBA to support protein synthesis. In experiment 2, five hundred seventy-six 1-d-old broilers were allotted to 12 dietary treatments (TRT) for a 21-d growth assay. A TSAA-deficient diet containing 0.70% total TSAA (TRT 1) was supplemented with 0.05, 0.10, 0.15, and 0.20% free HMTBA (TRT 2 to 5) to establish the standard Met response curve. Treatment 6 was analogous to TRT 2 but had an additional 160 ppm Zn, 80 ppm Cu, and 160 ppm Mn as sulfates. Treatments 7 to 12 were identical to TRT 2 but supplemented with 40 or 160 ppm Zn from Mintrex Zn, 20 or 80 ppm Cu from Mintrex Cu, or 40 or 160 ppm Mn from Mintrex Mn, respectively. For TRT 1 through 6, growth performance increased due to increasing Met addition (P < 0.01) but not to increasing inorganic trace minerals. For Mintrex Zn, Cu, and Mn (TRT 7 to 12), there was a linear increase in cumulative gain:feed ratio (P < 0.04), and for Mintrex Zn and Mn, there was a linear increase in cumulative gain (P < 0.03) to increasing Mintrex addition. A 1-slope broken-line model was used to calculate bioavailable Met activity from Mintrex for comparison with actual intake values. Results indicated that HMTBA from Mintrex was fully available as a Met source.

The objective of this study was to compare the gain-response curve to dietary levels of 2-hydroxy-4(methylthio) butanoic acid (HMTBA) and DL-Met (DLM) across 4 floor pen trials in which different diets were used. Six replicates of 38 or 41 birds per pen (trials 1 to 2 and 3 to 4, respectively) were used in a 2 x 3 factorial arrangement. A control with 12 replicates was also included. The 2 Met sources were fed at 3 equimolar levels equally spaced, with the highest level added at requirements from 1 to 48, 49, 43, or 49 d for trials 1, 2, 3, and 4, respectively. Commercial-type TSAA-deficient control diets contained sorghum, wheat, corn, or corn plus meat and bone meal for trials 1, 2, 3, and 4, respectively. Performance improved at all times for most parameters after supplementing with HMTBA or DLM (P0.05). No differences were found in the birds fed HMTBA or DLM at any age and trial (P0.05), except for trial 1, in which 17-d-old birds performed better when fed HMTBA than DLM (P0.05). In each trial, linear, quadratic, and exponential regressions were conducted upon the gain response of birds fed HMTBA and DLM separately. Equations with better goodness of fit were used to compare the estimated gain responses to feeding HMTBA vs. DLM. In 3 trials, the shape of the gain-response curve differed when feeding HMTBA vs. DLM. In trials 3 and 4, feeding HMTBA at commercial levels resulted in greater gain responses than DLM (P0.05), whereas, in trials 2 and 4, at very deficient levels, DLM-fed birds outperformed those fed HMTBA (P0.05). When the 4 trials were combined, the dose-response curve with the best goodness of fit was linear for HMTBA and quadratic for DLM. It can be concluded that the 2 Met sources have a different dose-response form, HMTBA could outperform DLM at commercial levels, and DLM could outperform HMTBA at deficient levels.

This experiment was conducted to compare the effects of feeding DL-2-hydroxy-4-(methylthio)butanoic acid (HMTBA) and DL-methionine (DLM) supplemented corn-soybean-cottonseed meal diets on growth performance, carcass composition, and muscle color of broilers. The trial was designed as a 2×3×2 factorial experiment, including two methionine (Met) sources (HMTBA and DLM), three equimolar graded levels of Met supplementation (i.e., 0.08, 0.16, and 0.24% in the starter diet and 0.07, 0.14, and 0.21% in the grower and finisher diets, respectively), and two sexes (male and female). Additionally, one basal diet for each sex was formulated to be limiting in Met to test the dosage response of increasing supplemental Met levels. Four hundred and twenty 10-d-old broilers were randomly allotted to 14 treatments (seven each for males and females), with five replicate pens per treatment and six chicks per pen. There was no difference (p>0.05) between the two Met sources in growth performance and muscle deposition of broilers throughout the whole experimental period (d 10 to 49). With the increasing Met supplementation levels, average daily gain was increased (quadratic; p

Karen J Wedekind,1 Josie A Coverdale,2 Thomas R Hampton,1 Cindy A Atwell,1 Roy H Sorbet,3 Jenea Lunnemann,1 Robert J Harrell,1 Laura Greiner,4 Nancy K Keith,5 Joseph L Evans,1 Junmei Zhao,1 Chris D Knight1 1Novus International, Inc., St Charles, MO, USA; 2Department of Animal Science, Texas A&M University, College Station, TX, USA; 3Certus International, Inc., Chesterfield, MO, USA; 4Innovative Swine Solution, Carthage, IL, USA; 5Keith Associates, Springfield, MO, USA Purpose: To determine the efficacy of an equine joint supplement STEADFAST® and/or its active components (Natural Eggshell Membrane [NEM®] and chelated trace minerals [CTM]) in horses with naturally occurring osteoarthritis or in a chemically induced osteoarthritis rat model. In addition, the efficacy of CTM vs inorganic trace minerals (ITM) (Zn, Mn, and Cu) in reducing culling rates in swine was evaluated. Methods: Horse trial: 16 mature horses with existing lameness were fed test joint supplement or placebo for 42 d. Lameness (American Association Equine Practitioner scoring system), serum biomarkers (C-terminal cross-linked telopeptide type II collagen [CTXII] and N-propeptide type IIA collagen [PIIANP]), and synovial fluid WBC were assessed biweekly. Rat trial: A chemically induced (monoiodoacetate [MIA]) osteoarthritis rat model was utilized. Rats were fed either negative control or joint supplement at 1% or 2% inclusion in Exp 1 (n=54) for 56 d. In Exp 2 (n=48), rats were fed control, NEM, CTM, or NEM + CTM for 42 d (28 d prefeed + Exp period). Rats were injected with MIA d29. Pain, knee swelling, and CTXII were measured post-MIA injection. Sow trial: Two farms with 6,400 sows each were fed ITM or 50:50 blend of ITM:CTM at equal TM levels for ~3 yr. Treatments were initiated at weaning through entry into the breeding herd. Sows remained on treatment until culled. Sow retention rate and reasons for removal were measured. Results: In horse and rat trials, chondromodulating effects of the joint supplement were observed: increased cartilage synthesis (PIIANP) or decreased cartilage degradation (CTXII). CTM + NEM decreased pain, swelling, and CTXII, compared to control and/or CTM or NEM alone (P30% with CTM supplementation (P

Data from the Galileo Probe in Jupiter descent indicated descent velocity oscillations as large as ±5 m/s on a height scale of a few km, which suggested gravity waves in the atmosphere between 4 and 20 bars (Seiff et al., 1998), an important observation for atmospheric stability and dynamics. But we now find these velocity fluctuations to be inconsistent with simultaneous measurements of mean accelerations, which were relatively steady. This conflict is resolved in favor of the accelerometers. The velocity fluctuations can be explained from digital uncertainties in the slow rate of pressure rise. However, the accelerometers did record higher frequency perturbations of up to 0.1g. Attributed to turbulence, these imply turbulent velocities from 0.3 to 5 m/s at scales of 10 to 40 m. However, they were at least partly a result of unsteady parachute aerodynamics.

The objective of this research was to evaluate the effect of early feeding on the development of the immune system in broiler chicks. A hydrated nutritional supplement formulated specifically for the first 2 to 3 days of post-hatch life was provided to the chicks on the day of hatch and the 2 subsequent days. The nutritional content of this supplement was optimized to achieve maximum performance at 6 wk following ad libitum consumption of starter feed and water. Control hatchlings received neither feed nor water. After the initial treatment, all birds were fed the same corn-soy diet ad libitum. Immune development was assessed by measuring bursa weight, biliary immunoglobulin A (IgA) levels, appearance of germinal centers in the cecal tonsils, and resistance to an oral challenge with coccidial oocysts. Data indicate that provision of the optimum balance of nutrients immediately after hatch result in heavier bursa weights, earlier appearance of biliary IgA and germinal centers, and an improved resistance to disease challenge.

The results of the nephelometer experiment conducted aboard the probe of the Galileo mission to Jupiter are presented. The tenuous clouds and sparse particulate matter in the relatively particle-free 5-μm “hot spot” region of the probe's descent were documented from about 0.46 bar to about 12 bars. Three regions of apparent coherent structure were noted, in addition to many indications of extremely small particle concentrations along the descent path. From the first valid measurement at about 0.46 bar down to about 0.55 bar, a feeble decaying lower portion of a cloud, corresponding with the predicted ammonia particle cloud, was encountered. A denser, but still very modest, particle structure was present in the pressure regime extending from about 0.76 bar to a distinctive base at 1.34 bars and is compatible with the expected ammonium hydrosulfide cloud. No massive water cloud was encountered, although below the second structure, a small, vertically thin layer at about 1.65 bars may be detached from the cloud above, but may also be water condensation, compatible with reported measurements of water abundance from other Galileo Mission experiments. A third small signal region, extending from about 1.9 to 4.5 bars, exhibited quite weak but still distinctive structure and, although the identification of the light scatterers in this region is uncertain, may also be a water cloud, perhaps associated with lateral atmospheric motion and/or reduced to a small mass density by atmospheric subsidence or other causes. Rough descriptions of the particle size distributions and cloud properties in these regions have been derived, although they may be imprecise because of the small signals and experimental difficulties. These descriptions document the small number densities of particles, the moderate particle sizes, generally in the slightly submicron to few micron range, and the resulting small optical depths, mass densities due to particles, column particle number loading, and column mass loading in the atmosphere encountered by the Galileo probe during its descent.

The objective of this research was to determine the efficacy of 2 types of adsorbents [hydrated sodium calcium aluminosilicates (HSCAS) vs. a combination of clay and yeast cell wall] in preventing aflatoxicosis in broilers. A total of 275 one-day-old birds were randomly divided into 11 treatments, with 5 replicate pens per treatment and 5 chicks per pen. The 11 treatments included 3 diets without any adsorbent containing either 0, 1, or 2 mg/kg of aflatoxin B1 (AFB1) plus 8 additional treatments employing 2 dietary levels of AFB1 (1 or 2 mg/kg), 2 different adsorbents [Solis (SO) and MTB-100 (MTB)], and 2 different levels of each absorbent (0.1 and 0.2%) in a 2×2×2 factorial arrangement. Solis is a mixture of different HSCAS and MTB is a combination of clay and yeast cell wall. Feed and water were provided ad libitum throughout the 21-d study period. Body weight gain and feed intake were depressed and relative liver weight was increased in chicks fed AFB1 compared with the positive control (P

The purpose of this paper was to compile all available literature comparing the relative performance of 2-hydroxy-4-methylthio butanoic acid (HMTBA) with DL-methionine (DLM) in broiler chickens and using multiple regression techniques, to estimate the predicted dose responses and relative performance of the 2 Met compounds for gain and feed conversion (FC). A database was developed that contained all available broiler studies in which HMTBA and DLM were both present in the same study; weight gain was recorded; Met addition, age of birds, and duration of study were defined; and an unsupplemented control treatment was present. Sixty-two references complied with these criteria and included 100 experiments with 427 observations for HMTBA and 411 for DLM. Multiple regression analysis of the database was used to identify the experimental and dietary conditions that contributed to the gain and FC responses of each source of Met activity. All identified variables contributed similarly to each Met source prediction model and both gain and FC models described a quadratic dose response. Under the average conditions of the database, the predicted responses for gain and FC models did not significantly differ between HMTBA and DLM. However, a trend was observed (P less than or equal to 0.1) for the peak gain response for HMTBA to be numerically greater than DLM, suggesting benefits of HMTBA over DLM in the region of supplementation that is commercially relevant. The experimental and nutritional conditions that contribute to the response to HTMBA and DLM were identified and are discussed in the paper. This statistical approach provided a means to summarize the results obtained from a multitude of studies conducted over the last 5 decades and has provided a meaningful estimate of the relative performance of the 2 sources of Met activity. The lack of differences between the 2 predicted models under experimental and commercial conditions supports an overall conclusion of equal performance of DLM and HTMBA when compared on an equal molar basis.

The atmosphere of Jupiter has a complex circulation which, until recently, has been observable only at the cloud tops1,2; the mechanisms driving the winds, and the nature of the interior circulation, remained unknown3. Recent analyses4,5,6 of the radio signal from the Galileo probe, obtained during its descent into the jovian atmosphere, have suggested a vigorous interior circulation below the 4-bar level. Here we report an independent measurement of the winds below the cloud tops, making use of the data obtained by the two accelerometers on the descending probe. We find evidence for two distinct wind regimes, in general agreement with the Doppler radio measurements: a region of wind shear between 1 and 4 bar, where the wind speed increases dramatically with depth; and then a region of constant high-velocity winds down to at least the 17-bar level.

Seven hundred and twenty hatchling broilers were allotted to 12 treatment groups. Groups 1 and 2 were fasted for 48 h posthatch; groups 3 and 4 were fasted for 48 h followed by ad libitum access to a 1% glutamine (Gln) diet; groups 5 and 6 had ad libitum access to a common diet; groups 7 and 8 had access to a 1% Gln diet posthatch; groups 9 and 10 were fed regular Oasis hatchling supplement; and groups 11 and 12 were fed Oasis sprayed with 1% Gln for the first 48 h posthatch. The birds in treatment groups 2, 4, 6, 8, 10, 11, and 12 were vaccinated with Eimeria maxima posthatch, and all birds were orally challenged with high dose E. maxima on d 22. During the first 2 wk, birds in group 7 had the highest gain and feed efficiency among treatments (P0.01). Compared with birds in the nonGln groups, birds in the Gln group had higher gain, feed efficiency, and livability (P0.05). Among the Fast (groups 1 to 4), Feed (groups 5 to 8), and Oasis (groups 9 to 12) groups, birds in the Feed groups had the highest gain during d 0 to 21 (P0.01). During d 22 to 28, birds in the Fast groups had the lowest BW and livability (P0.01), and the nonvaccinated birds had lower gain and feed efficiency relative to vaccinated birds (P0.01). Birds in the Feed and Oasis groups had higher villus height (VH) of mid small intestine than Fast groups at d 2 and 7 (P0.05), and nonvaccinated birds had higher VH than vaccinated birds (P0.01) at d 7 after hatch. On d 14, there were differences in serum interferon-gamma (P0.05) levels among treatments. During d 22 to 28, vaccinated birds had lower lesion scores in the mid small intestine than nonvaccinated birds (P0.01), and birds in the Feed or Oasis groups had lower lesion scores compared with the Fast groups (P0.02). These results indicated the importance of immediate access to feed posthatch, the beneficial effects of feeding Oasis hatching supplement and Gln after hatch, as well as the necessity of the vaccination program against coccidiosis challenge.

Temperatures and pressures measured by the Galileo probe during parachute descent into Jupiter's atmosphere essentially followed the dry adiabat between 0.41 and 24 bars, consistent with the absence of a deep water cloud and with the low water content found by the mass spectrometer. From 5 to 15 bars, lapse rates were slightly stable relative to the adiabat calculated for the observed H2/He ratio, which suggests that upward heat transport in that range is not attributable to simple radial convection. In the upper atmosphere, temperatures of >1000 kelvin at the 0.01-microbar level confirmed the hot exosphere that had been inferred from Voyager occultations. The thermal gradient increased sharply to 5 kelvin per kilometer at a reconstructed altitude of 350 kilometers, as was recently predicted. Densities at 1000 kilometers were 100 times those in the pre-encounter engineering model.

Animal feeding studies were conducted with rats, broiler chickens, catfish and dairy cows as part of a safety assessment program for a soybean variety genetically modified to tolerate in-season application of glyphosate. These studies were designed to compare the feeding value (wholesomeness) of two lines of glyphosate-tolerant soybeans (GTS) to the feeding value of the parental cultivar from which they were derived. Processed GTS meal was incorporated into the diets at the same concentrations as used commercially; diary cows were fed 10 g/100 g cracked soybeans in the diet, a level that is on the high end of what is normally fed commercially. In a separate study, laboratory rats were fed 5 and 10 g unprocessed soybean meal 100 g diet. The study durations were 4 wk (rats and dairy cows), 6 wk (broilers) and 10 wk (catfish). Growth, feed conversion (rats, catfish, broilers), fillet composition (catfish), and breast muscle and fat pad weights (broilers) were compared for animals fed the parental and GTS lines. Milk production, milk composition, rumen fermentation and nitrogen digestibility were also compared for dairy cows. In all studies, measured variables were similar for animals fed both GTS lines and the parental line, indicating that the feeding value of the two GTS lines is comparable to that of the parental line. These studies support detailed compositional analysis of the GTS seeds, which showed no meaningful differences between the parental and GTS lines in the concentrations of important nutrients and antinutrients. They also confirmed the results of other studies that demonstrated the safety of the introduced protein, a bacterial 5-enolpyruvyl-shikimate-3-phosphate synthase from Agrobacterium sp. strain CP4.

The objective of these experiments was to study the effect of intermittent high temperature (IHT) on performance of broilers fed different sources of methionine activity. Two 20-d experiments were conducted in which individually caged 28-d-old cockerels were exposed to a 5-d period of constant high temperature (HT, 30 to 32 C) followed by an equal period of thermoneutral (TN) temperatures (22 C) with the 10-d temperature cycle repeated twice. Birds held at 22 C were TN controls. Grower diets contained 3,275 kcal/kg ME and 20% CP and were supplemented with either 2-hydroxy-4-(methylthio)butanoic acid (HMB, Alimet Feed Supplement) or DL-Met up to a maximum of .88 to .90% total sulfur amino acids (TSAA). In Experiment 1, gain:feed but not average daily gain was greater (P < .05) for HMB than DL-Met birds subjected to IHT (.451 vs .413, respectively), but no treatment differences were observed for TN birds. Results of Experiment 2 demonstrated a linear response to HMB and DL-Met dose in TN (P < .01); however, only HMB-supplemented birds responded similarly in IHT (P < .01). These results are consistent with lower availability of DL-Met as a result of IHT. In vitro experiments indicated that capacity for uptake of DL-Met into intestinal epithelial cells was reduced in heat-stressed birds. Uptake of D-Met was more severely affected than was L-Met. Consequently, a third performance experiment compared the ability of D-Met and L-Met to support growth under conditions of HT. The results indicated that the effect of HT on broiler performance was mediated through reduced utilization of D-Met.

A three-axis short-period seismometer is now operating on Mars in the Utopia Planitia region. The noise background correlates well with wind gusts. Although no quakes have been detected in the first 60 days of observation, it is premature to draw any conclusions about the seismicity of Mars. The instrument is expected to return data for at least 2 years.

A three-axis short-period seismometer has been operating on the surface of Mars in the Utopia Planitia region since September 4, 1976. During the first 5 months of operation, approximately 640 hours of high quality data, uncontaminated by lander or wind noise, have been obtained. The detection threshold is estimated to be magnitude 3 to about 200 km and about 6.5 for the planet as a whole. No large events have been seen during this period, a result indicating that Mars is less seismically active than earth. Wind is the major source of noise during the day, although the noise level was at or below the sensitivity threshold of the seismometer for most of the night during the early part of the mission. Winds and therefore the seismic background started to intrude into the nighttime hours starting on sol 119 (a sol is a Martian day). The seismic background correlates well with wind velocity and is proportional to the square of the wind velocity, as is appropriate for turbulent flow. The seismic envelope power spectral density is proportional to frequency to the -0.66 to -0.90 power during windy periods. A possible local seismic event was detected on sol 80. No wind data were obtained at the time, so a wind disturbance cannot be ruled out. However, this event has some unusual characteristics and is similar to local events recorded on earth through a Viking seismometer system. If it is interpreted as a natural seismic event, it has a magnitude of 3 and a distance of 110 km. Preliminary interpretation of later arrivals in the signal suggest a crustal thickness of 15 km at the Utopia Planitia site which is within the range of crustal models derived from the gravity field. More events must be recorded before a firm interpretation can be made of seismicity or crustal structure. One firm conclusion is that the natural background noise on Mars is low and that the wind is the prime noise source. It will be possible to reduce this noise by a factor of 10^3 on future missions by removing the seismometer from the lander, operation of an extremely sensitive seismometer thus being possible on the surface.

During analysis of early events in the infection and nodulation of Vicia hirsuta roots inoculated with normal and mutant strains of Rhizobium leguminosarum and strains containing cloned nodulation (nod) genes, a number of novel observations were made. (i) Alternating zones of curled and straight root hairs were seen on roots of V. hirsuta inoculated with the wild-type strain of R. leguminosarum. This phasing of root hair curling was not seen if plants were grown under continuous light or continuous dark conditions. (ii) Reduced nodulation and delayed nodule initiation was observed with a strain carrying a Tn5 mutation in the nodE gene. In addition the phased root hair curling was absent, and root hair curling was observed along the length of the root. (iii) The nodABC genes cloned on a multicopy plasmid in a wild-type strain inhibited nodulation but induced a continuous root hair curling response. Those few nodules that eventually formed were found to contain bacteria which had lost the plasmid carrying the nodABC genes. (iv) With a strain of Rhizobium cured of its indigenous symbiotic plasmid, but containing the cloned nodABCDEF genes, continuous root hair curling on V. hirsuta was observed. However, no infection threads were observed, and surprisingly, it did appear that initial stages of nodule development occurred. Observations of thin sections of these early developing nodules indicated that early nodule meristematic divisions may have occurred but that no bacteria were found within the nodules and no infection threads were observed either within the nodule bumps or within any of the root hairs. It was concluded that for normal infections to occur, precise regulation of the nod genes is required and that overexpression of the root hair curling genes inhibits the normal infection process.

SUMMARY This review will examine the early stages of infection of legume roots by strains of Rhizobium that induce nitrogen-fixing nodules. The object is to show that, at least in terms of ultrastructure, the interactions between the plant and the rhizobia occur at the cell surface interface between these organisms. This situation exists at all stages, from the time the bacteria attach to the surface of the root hairs to the time they occur as the nitrogen-fixing form in the cytoplasm of the nodule cells, enclosed by peribacteroid membranes.

Results from the aeroshell-mounted neutral mass spectrometer on Viking I indicate that the upper atmosphere of Mars is composed mainly of CO(2) with trace quantities of N(2), Ar, O, O(2), and CO. The mixing ratios by volume relative to CO(2) for N(2), Ar, and O(2) are about 0.06, 0.015, and 0.003, respectively, at an altitude near 135 kilometers. Molecular oxygen (O(2)(+)) is a major component of the ionosphere according to results from the retarding potential analyzer. The atmosphere between 140 and 200 kilometers has an average temperature of about 180 degrees +/- 20 degrees K. Atmospheric pressure at the landing site for Viking 1 was 7.3 millibars at an air temperature of 241 degrees K. The descent data are consistent with the view that CO(2) should be the major constituent of the lower martian atmosphere.

Considerable morbidity and mortality are associated with infections of vascular prosthetic grafts. Most vascular surgeons believe that once vascular prosthetic graft infections occur, any form of therapy short of removal of the prosthesis will fail. Although recent reports have noted the successful conservative treatment of vascular prosthetic graft infections in the groin, most surgeons have been hesitant to use such therapy for aortic prosthetic graft infections. We describe an 88-year-old woman with an aortic graft infection 2 months after abdominal aortic aneurysmectomy who was treated successfully with local debridement and continuous irrigation of the graft bed with providone-iodine solution. Such local therapy is an alternative and sometimes effective method of managing patients with infected aortic grafts in whom the risk of resection of the prosthesis and revascularization would be prohibitive.

The region of the Rhizobium leguminosarum plasmid pRL1JI involved in nodulation and nitrogen fixation has been cloned on a series of four overlapping cosmid clones. These clones represent ˜60 kb of pRL1JI DNA on which a series of Tn5-induced fix and nod alleles have been identified, with the two most distant alleles being separated by ˜45 kb of DNA. The mutant alleles fell into three groups, two clusters of fix alleles separated by one cluster of nod alleles. Within one group of fix alleles, DNA homologous to the nifA gene of Klebsiella pneumoniae has been identified, whereas the pRL1JI DNA homologous to the K. pneumoniae nitrogenase genes is present within the other group of fix alleles.