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Dietary intake of selenium has been implicated in a wide range of health issues, including aging, heart disease and cancer. Selenium deficiency, which can reduce selenoprotein levels, has been associated with several striated muscle pathologies. To investigate the role of selenoproteins in skeletal muscle biology, we used a transgenic mouse (referred to as [i.sup.6][A.sup.-]) that has reduced levels of selenoproteins due to the introduction and expression of a dominantly acting mutant form of selenocysteine transfer RNA (tRN[A.sup.[Ser]sec]). As a consequence, each organ contains reduced levels of most selenoproteins, yet these mice are normal with regard to fertility, overall health, behavior and blood chemistries. In the present study, although skeletal muscles from [i.sup.6][A.sup.-] mice were phenotypically indistinguishable from those of wild-type mice, plantaris muscles were ~50% heavier after synergist ablation, a model of exercise overload. Like muscle in wild-type mice, the enhanced growth in the [i.sup.6][A.sup.-] mice was completely blocked by inhibition of the mammalian target of rapamycin (mTOR) pathway. Muscles of transgenic mice exhibited increased site-specific phosphorylation on both Akt and p70 ribosomal S6 kinase ([p70.sup.S6k]) (P- KEY WORDS: * selenium * Akt/PKB * transgenic * signaling * p70 ribosomal S6 kinase

Consumption of lycopene, the predominant carotenoid in tomatoes and tomato products, is associated with reduced prostate cancer risk. The purpose of this study was to measure the pharmacokinetics and tissue distribution of lycopene after oral administration to male dogs. After single doses of 10, 30 and 50 mg/kg body weight (BW) lycopene to 2 dogs/dose, the mean half-life was 36 h and the plasma systemic exposure levels (AU[C.sub.0-[infinity]], area under the curve) after the 30 and 50 mg/kg BW doses were similar. In a repeat dose study, 30 mg/(kg BW * d) administered orally to six dogs for 28 d resulted in steady-state plasma concentrations between 785 and 997 nmol/L lycopene. Apparent clearance, volume of distribution and apparent elimination half-life were 2.29 L/(h * kg), 96 L/kg and 30.5 h, respectively. Dogs were killed 1 or 5 d after the last dose and 23 tissues were collected for lycopene analysis. Lycopene concentrations were highest in liver, adrenals, spleen, lymph nodes and intestinal tissues. Liver lycopene concentrations were 66 and 91 nmol/g 1 and 5 d after cessation of treatment, respectively. Prostate lycopene concentrations were < 0.2 nmol/g both 1 and 5 d after dosing ceased ( KEY WORDS: * carotenoid * prostate * cancer * chemoprevention * canine

Serum concentrations of several nutrients were measured in 12 captive wild felid species including caracal (Felis caracal), cheetah (Acinonyx jubatus), cougar (Fells concolor), fishing cat (Fells viverrinus), leopard (Panthera pardus), lion (Panthera leo), ocelot (Fells pardalis), pallas cat (Fells manul), sand cat (Fells margarita), serval (Felis serval), snow leopard (Panthera uncia) and tiger (Panthera tigris). Diet information was collected for these animals from each participating zoo (Brookfield Zoo, Fort Worth Zoo, Lincoln Park Zoological Gardens and North Carolina Zoological Park). The nutritional composition of the diets at each institution met the probable dietary requirements for each species except for the pallas cat. Blood samples were collected from each animal (n = 69) and analyzed for lipids (total cholesterol, triacylglycerides, HDL cholesterol and LDL cholesterol), vitamin D metabolites [25-hydroxycholecalciferol (25(OH)D) and 1,25-dihydroxycholecalciferol (1,25(OH)2D)], vitamin A (retinol, retinyl stearate and retinyl palmitate), vitamin E ([alpha]- and [gamma]-tocopherol) and selected carotenoids. Species differences were found for all except triacylglycerides and 1,25(OH)2D. Genus differences were found for retinol, retinyl palmitate, retinyl stearate, [gamma]-tocopherol and [beta]-carotene. Circulating nutrient concentrations for many of the species in this study have not been reported previously and most have not been compared with the animals' dietary intakes. The large number of animals analyzed provides a substantial base for comparing the serum nutrient concentrations of healthy animals, for both wild and captive exotic species. KEY WORDS: * Fells * lipids * vitamin A * vitamin D * vitamin E

Age-related macular degeneration (ARMD) is inversely associated with the accumulation of lutein + zeaxanthin in the macula, but higher lutein intakes are inconsistently related to reduced risk of ARMD in epidemiologic studies. Resolution of efficacy awaits clinical trials designed with knowledge of lutein supplement pharmacokinetics. Lutein bioavailability was determined for lutein diester and unesterified lutein formulations as they might be incorporated into dietary supplements. Healthy subjects (n = 18) consumed a single dose of each formulation (either 0.5 or 0.67 [micro]mol lutein/kg body, 10 and 8 subjects, respectively) in random order, and the appearance of free lutein + zeaxanthin was measured in serum from 0 to 408 h. Areas under the serum concentration x time curves (AUC), as a measure of bioavailability, were independent of gender, body mass index and lutein dose. The lutein diester formulation was 61.6% more bioavailable than the unesterified lutein formulation with higher mean AUC, maximum serum concentration and ascending slope (P < 0.05). The AUC was greater in 14 of 18 subjects when they consumed the lutein diester formulation. Comparison with data from previous studies suggested that dissolution was a greater limitation to bioavailability than lutein ester hydrolysis because an oil-solubilized unesterified lutein preparation, given at 0.5 [micro]mol/kg body, resulted in greater mean peak concentrations and AUC compared with either the unesterified or lutein diester formulations used in our study. In conclusion, the lutein diester formulation poses no impediment to lutein bioavailability at the doses tested, but formulation dissolution is an important factor in lutein bioavailability and should be evaluated before a supplement and dose are selected for use in clinical trials. J. Nutr. 132: 3668-3673, 2002. KEY WORDS: * lutein * lutein ester * bioavailability * humans

We examined the concentrations of five carotenoids in the serum and diet of a population-based sample of 400 individuals to determine what physiologic and lifestyle factors were related to serum carotenoid concentrations, how these relationships differed among the carotenoids, and if these relationships reflected differences in carotenoid intake. Lower serum concentrations of [Alpha]-carotene, [Beta]-carotene, [Beta]-cryptoxanthin, and lutein + zeaxanthin generally were associated with male gender, smoking, younger age, lower non-HDL cholesterol, greater ethanol consumption and higher body mass index. Serum lycopene generally was not related to these factors, but lower lycopene levels were associated with older age and lower non-HDL cholesterol. Only the hydrocarbon carotenoids ([Alpha]- and [Beta]carotene and lycopene) were directly associated with HDL cholesterol. The associations of some factors (gender, age, smoking, and ethanol intake) with serum carotenoids were similar to the associations of these factors with levels in the diet, indicating that serum carotenoids may reflect the influence of these factors on carotenoid intake. Consistent with this notion, correlations between serum and dietary carotenoids did not differ between smokers and nonsmokers. Other factors (HDL and non-HDL cholesterol and body mass index) associated with carotenoids in the serum were not associated with carotenoid intake, indicating that physiologic conditions that affect the absorption, storage, and utilization of carotenoids may influence these associations. These physiologic and behavioral correlates of carotenoids could explain or modify associations of carotenoids with chronic diseases. INDEXING KEY WORDS: carotenoids; humans

Assesses the oxidative DNA damage in prostate cancer patients consuming tomato sauce-based foods. Accumulation of lycopene in the prostate tissue; Analysis of lycopene concentration in tomato sauce; Method and design of the study.

The article focuses on the selection of surrogate endpoint biomarkers to evaluate the efficacy of lycopene/tomatoes for the prevention/progression of prostate cancer. The use of surrogate endpoint biomarkers (SEBs) is essential to the crafting of cost-effective clinical trials to evaluate not only the efficacy of lycopene/tomatoes but also combinations of bioactive compounds. The closer the SEB is in the pathway to prostate cancer expression or progression, the more likely its predictive value. High-grade prostate intraepithelial neoplasia, microvessel density, and prostate-specific antigen have been exploited for these purposes, and their sensitivity and specificity in predicting clinically relevant prostate cancer can be determined. The pathways modulated by tomato bioactive ingredients can be determined through tissue culture and animal studies. A number of possible bioactive compounds are contained in tomato. consumption. Lycopene and quercetin are the likeliest candidates for prostate cancer mediation. They are predominant compounds in tomato and have been shown in animal studies to reduce cancer.

We examined the effects of aerobic exercise on postprandial metabolism in obese glucose tolerant and intolerant adults. Subjects included African-American (n=24; 9f; 5m), Caucasian (n=12; 8f; 4m) and Hispanic (n=8; 5f; 3m) adults classified into 3 groups (type 2 diabetes [DM] [n = 13]; impaired glucose tolerance [IGT] [n = 7]; normal glucose tolerance [NGT] [n = 24]) based on oral glucose tolerance tests. Subjects were similar in age (44.5 ± 8.5 years [mean ± SD]); BMI (35.2 … 15.1 (kg/m²); total body fat mass (36.7 ± 6.7 kg); total body lean tissue mass (62.8 ± 11.5 kg); and insulin sensitivity (SI) [3.0 ± 1.6 x 10[sup -4] min[sup -1] ⋅ (µU/ml)]; type 2 DM subjects had lower acute insulin responses to glucose (AIRg) and disposition indexes (DI) than those with NGT (p <0.05). Subjects participated in an exercise (E) and non-exercise (NE) protocol. During the E protocol subjects walked on the treadmill for 30 minutes at 65% V0[sub 2] Max; during the NE protocol subjects rested for 30 minutes. Three standard meals were consumed following exercise/rest; glucose and insulin responses were measured at ½ hour prior and ½ 1, 2 and 3 hours following each meal. Percent changes in insulin and glucose responses from baseline were calculated at each timepoint. The data were analyzed using repeated-measures ANOVA. There were between-group differences in plasma glucose responses (type 2 DM vs. NGT) following breakfast (1, 2 hours); following lunch (type 2 DM vs. IGT) (1 hour); following dinner (1 hour) (p <0.05). In addition, them were within-group interactions (group by exercise) following breakfast (2, 3 hours) and dinner (2, 3 hours) (p < 0.05). There were between-group differences in plasma insulin responses (type 2 DM vs. NGT) immediately following exercise (p < 0.05); following breakfast (type 2 DM vs. NGT; type 2 DM vs. IGT) (½, 1 hours) (p < 0.05). In addition, there was an interaction effect (group by exercise) immediately following exercise (p < 0.04); and an interaction effect (exercise) 30 minutes following exercise. These data suggest that moderate intensity aerobic exercise impacts insulin responses during the early post exercise recovery period; such responses are associated with changes in postprandial glycemic responses throughout the entire day. Further studies will examine the impact of higher exercise intensity and/or greater caloric expenditure on postprandial responses. [ABSTRACT FROM AUTHOR]