Search

Your search keyword '"Boussouar, Fayçal"' showing total 36 results
Start Over Author "Boussouar, Fayçal" Search Limiters Available in Library Collection
36 results on '"Boussouar, Fayçal"'

1. Nucleoside diphosphate kinases 1 and 2 regulate a protective liver response to a high-fat diet

Author

Iuso, Domenico, primary, Garcia-Saez, Isabel, additional, Couté, Yohann, additional, Yamaryo-Botté, Yoshiki, additional, Boeri Erba, Elisabetta, additional, Adrait, Annie, additional, Zeaiter, Nour, additional, Tokarska-Schlattner, Malgorzata, additional, Jilkova, Zuzana Macek, additional, Boussouar, Fayçal, additional, Barral, Sophie, additional, Signor, Luca, additional, Couturier, Karine, additional, Hajmirza, Azadeh, additional, Chuffart, Florent, additional, Bourova-Flin, Ekaterina, additional, Vitte, Anne-Laure, additional, Bargier, Lisa, additional, Puthier, Denis, additional, Decaens, Thomas, additional, Rousseaux, Sophie, additional, Botté, Cyrille, additional, Schlattner, Uwe, additional, Petosa, Carlo, additional, and Khochbin, Saadi, additional

Published
2023
Full Text
View/download PDF

2. ATAD2 controls chromatin-bound HIRA turnover

Author

Wang, Tao, Perazza, Daniel, Boussouar, Fayçal, Cattaneo, Matteo, Bougdour, Alexandre, Chuffart, Florent, Barral, Sophie, Vargas, Alexandra, Liakopoulou, Ariadni, Puthier, Denis, Bargier, Lisa, Morozumi, Yuichi, Jamshidikia, Mahya, Garcia-Saez, Isabel, Petosa, Carlo, Rousseaux, Sophie, Verdel, André, Khochbin, Saadi, Wang, Tao, Perazza, Daniel, Boussouar, Fayçal, Cattaneo, Matteo, Bougdour, Alexandre, Chuffart, Florent, Barral, Sophie, Vargas, Alexandra, Liakopoulou, Ariadni, Puthier, Denis, Bargier, Lisa, Morozumi, Yuichi, Jamshidikia, Mahya, Garcia-Saez, Isabel, Petosa, Carlo, Rousseaux, Sophie, Verdel, André, and Khochbin, Saadi

Abstract

Taking advantage of the evolutionary conserved nature of ATAD2, we report here a series of parallel functional studies in human, mouse, and Schizosaccharomyces pombe to investigate ATAD2’s conserved functions. In S. pombe, the deletion of ATAD2 ortholog, abo1, leads to a dramatic decrease in cell growth, with the appearance of suppressor clones recovering normal growth. The identification of the corresponding suppressor mutations revealed a strong genetic interaction between Abo1 and the histone chaperone HIRA. In human cancer cell lines and in mouse embryonic stem cells, we observed that the KO of ATAD2 leads to an accumulation of HIRA. A ChIP-seq mapping of nucleosome-bound HIRA and FACT in Atad2 KO mouse ES cells demonstrated that both chaperones are trapped on nucleosomes at the transcription start sites of active genes, resulting in the abnormal presence of a chaperone-bound nucleosome on the TSS-associated nucleosome-free regions. Overall, these data highlight an important layer of regulation of chromatin dynamics ensuring the turnover of histone-bound chaperones.

Published
2023

3. Genome-wide nucleosome specificity and function of chromatin remodellers in ES cells

Author

de Dieuleveult, Maud, Yen, Kuangyu, Hmitou, Isabelle, Depaux, Arnaud, Boussouar, Fayçal, Dargham, Daria Bou, Jounier, Sylvie, Humbertclaude, Hélène, Ribierre, Florence, Baulard, Céline, Farrell, Nina P., Park, Bongsoo, Keime, Céline, Carrière, Lucie, Berlivet, Soizick, Gut, Marta, Gut, Ivo, Werner, Michel, Deleuze, Jean-François, Olaso, Robert, Aude, Jean-Christophe, Chantalat, Sophie, Pugh, B. Franklin, and Gérard, Matthieu

Published
2016
Full Text
View/download PDF

4. ATAD2 controls chromatin-bound HIRA turnover

Author

Wang, Tao, Perazza, Daniel, Boussouar, Fayçal, Cattaneo, Matteo, Bougdour, Alexandre, Chuffart, Florent, Barral, Sophie, Vargas, Alexandra, Liakopoulou, Ariadni, Puthier, Denis, Bargier, Lisa, Morozumi, Yuichi, Jamshidikia, Mahya, Garcia-Saez, Isabel, Petosa, Carlo, Rousseaux, Sophie, Verdel, A, Khochbin, Saadi, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Theories and Approaches of Genomic Complexity (TAGC), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Transscriptomics and Genomics of Marseille-Luminy (TGML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Nara Institute of Science and Technology, Groupe Épigénétique et voies moléculaires / Epigenetics and Molecular Pathways Group (IBS-EPIGEN), Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), and Khochbin, Saadi

Subjects
Genotype, Cell Cycle Proteins, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Transfection, Gene Knockout Techniques, Mice, Schizosaccharomyces, Animals, Humans, Histone Chaperones, Research Articles, Cell Proliferation, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Mouse Embryonic Stem Cells, Hep G2 Cells, Nucleosomes, DNA-Binding Proteins, ATPases Associated with Diverse Cellular Activities, Schizosaccharomyces pombe Proteins, Microorganisms, Genetically-Modified, Gene Deletion, Research Article, HeLa Cells, Signal Transduction, Transcription Factors
Abstract

Parallel study of multiple model systems points to the evolutionary conserved protein ATAD2, as a critical histone chaperone auxiliary factor., Taking advantage of the evolutionary conserved nature of ATAD2, we report here a series of parallel functional studies in human, mouse, and Schizosaccharomyces pombe to investigate ATAD2’s conserved functions. In S. pombe, the deletion of ATAD2 ortholog, abo1, leads to a dramatic decrease in cell growth, with the appearance of suppressor clones recovering normal growth. The identification of the corresponding suppressor mutations revealed a strong genetic interaction between Abo1 and the histone chaperone HIRA. In human cancer cell lines and in mouse embryonic stem cells, we observed that the KO of ATAD2 leads to an accumulation of HIRA. A ChIP-seq mapping of nucleosome-bound HIRA and FACT in Atad2 KO mouse ES cells demonstrated that both chaperones are trapped on nucleosomes at the transcription start sites of active genes, resulting in the abnormal presence of a chaperone-bound nucleosome on the TSS-associated nucleosome-free regions. Overall, these data highlight an important layer of regulation of chromatin dynamics ensuring the turnover of histone-bound chaperones.

Published
2021

7. ATAD2 controls chromatin-bound HIRA turnover.

Author

Tao Wang, Perazza, Daniel, Boussouar, Fayçal, Cattaneo, Matteo, Bougdour, Alexandre, Chuffart, Florent, Barral, Sophie, Vargas, Alexandra, Liakopoulou, Ariadni, Puthier, Denis, Bargier, Lisa, Yuichi Morozumi, Jamshidikia, Mahya, Garcia-Saez, Isabel, Petosa, Carlo, Rousseaux, Sophie, Verdel, André, and Khochbin, Saadi

Published
2021
Full Text
View/download PDF

12. Nut Directs p300-Dependent, Genome-Wide H4 Hyperacetylation in Male Germ Cells

Author

Shiota, Hitoshi, primary, Barral, Sophie, additional, Buchou, Thierry, additional, Tan, Minjia, additional, Couté, Yohann, additional, Charbonnier, Guillaume, additional, Reynoird, Nicolas, additional, Boussouar, Fayçal, additional, Gérard, Matthieu, additional, Zhu, Mingrui, additional, Bargier, Lisa, additional, Puthier, Denis, additional, Chuffart, Florent, additional, Bourova-Flin, Ekaterina, additional, Picaud, Sarah, additional, Filippakopoulos, Panagis, additional, Goudarzi, Afsaneh, additional, Ibrahim, Ziad, additional, Panne, Daniel, additional, Rousseaux, Sophie, additional, Zhao, Yingming, additional, and Khochbin, Saadi, additional

Published
2018
Full Text
View/download PDF

13. Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells

Author

Morozumi, Yuichi, Boussouar, Fayçal, Tan, Minjia, Chaikuad, Apirat, Jamshidikia, Mahya, Colak, Gozde, He, Huang, Nie, Litong, Petosa, Carlo, De Dieuleveult, Maud, Curtet, Sandrine, Vitte, Anne-Laure, Rabatel, Clothilde, Debernardi, Alexandra, François-Loïc, Cosset, Verhoeyen, Els, Emadali, Anouk, Schweifer, Norbert, Gianni, Davide, Gut, Marta, Guardiola, Philippe, Rousseaux, Sophie, Gérard, Matthieu, Knapp, Stefan, Zhao, Yingming, Khochbin, Saadi, Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), The Chemical Proteomics Center and State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Structural Genomics Consortium, University of Oxford, Nuffield Department of Clinical Medicine [Oxford], Ben May Department of Cancer Research, The University of Chicago Medicine, Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Virus enveloppés, vecteurs et immunothérapie – Enveloped viruses, Vectors and Immuno-therapy (EVIR), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Equipe 'Contrôle Métabolique des Morts Cellulaires' (INSERM U1065 - C3M), Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Boehringer Ingelheim RCV, Centre for Genomic Regulation [Barcelona] (CRG), Universitat Pompeu Fabra [Barcelona] (UPF)-Centro Nacional de Analisis Genomico [Barcelona] (CNAG), Centre de Recherche en Cancérologie Nantes-Angers (CRCNA), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM)-Hôtel-Dieu de Nantes-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Laennec-Centre National de la Recherche Scientifique (CNRS)-Faculté de Médecine d'Angers-Centre hospitalier universitaire de Nantes (CHU Nantes), Institut d'oncologie/développement Albert Bonniot de Grenoble ( INSERM U823 ), Université Joseph Fourier - Grenoble 1 ( UJF ) -CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale ( INSERM ), University of Oxford [Oxford], Target Discovery Institute (TDI), Nuffield Department of Clinical Medicine, Institut de biologie structurale ( IBS - UMR 5075 ), Université Joseph Fourier - Grenoble 1 ( UJF ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Grenoble Alpes ( UGA ), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ), Centre International de Recherche en Infectiologie ( CIRI ), Centre National de la Recherche Scientifique ( CNRS ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-École normale supérieure - Lyon ( ENS Lyon ), Centre for Genomic Regulation [Barcelona] ( CRG ), Universitat Pompeu Fabra [Barcelona]-Centro Nacional de Analisis Genomico [Barcelona] ( CNAG ), Inserm u892, Centre de Recherches en Cancérologie, Nantes, Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Adenosine Triphosphatases, Male, Proteomics, histone turnover, Chromatin Immunoprecipitation, germ cells, Genome, Pax3, [ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], FACT, epidrug, Acetylation, Cell Differentiation, Article, Chromatin, Nucleosomes, DNA-Binding Proteins, histone chaperone, ATPases Associated with Diverse Cellular Activities, Humans, cancer drug target, Embryonic Stem Cells, Cell Proliferation, Protein Binding
Abstract

International audience; Although the conserved AAA ATPase and bromodomain factor, ATAD2, has been described as a transcriptional co-activator upregulated in many cancers, its function remains poorly understood. Here, using a combination of ChIP-seq, ChIP-proteomics, and RNA-seq experiments in embryonic stem cells where Atad2 is normally highly expressed, we found that Atad2 is an abundant nucleosome-bound protein present on active genes, associated with chromatin remodelling, DNA replication, and DNA repair factors. A structural analysis of its bromodomain and subsequent investigations demonstrate that histone acetylation guides ATAD2 to chromatin, resulting in an overall increase of chromatin accessibility and histone dynamics, which is required for the proper activity of the highly expressed gene fraction of the genome. While in exponentially growing cells Atad2 appears dispensable for cell growth, in differentiating ES cells Atad2 becomes critical in sustaining specific gene expression programmes, controlling proliferation and differentiation. Altogether, this work defines Atad2 as a facilitator of general chromatin-templated activities such as transcription.

Published
2016

14. Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells

Author

Barral, Sophie, primary, Morozumi, Yuichi, additional, Tanaka, Hiroki, additional, Montellier, Emilie, additional, Govin, Jérôme, additional, de Dieuleveult, Maud, additional, Charbonnier, Guillaume, additional, Couté, Yohann, additional, Puthier, Denis, additional, Buchou, Thierry, additional, Boussouar, Fayçal, additional, Urahama, Takashi, additional, Fenaille, François, additional, Curtet, Sandrine, additional, Héry, Patrick, additional, Fernandez-Nunez, Nicolas, additional, Shiota, Hitoshi, additional, Gérard, Matthieu, additional, Rousseaux, Sophie, additional, Kurumizaka, Hitoshi, additional, and Khochbin, Saadi, additional

Published
2017
Full Text
View/download PDF

15. Bromodomain-dependent stage-specific male genome programming by Brdt

Author

Gaucher, Jonathan, Boussouar, Fayçal, Montellier, Emilie, Curtet, Sandrine, Buchou, Thierry, Bertrand, Sarah, Hery, Patrick, Jounier, Sylvie, Depaux, Arnaud, Vitte, Anne-Laure, Guardiola, Philippe, Pernet, Karin, Debernardi, Alexandra, Lopez, Fabrice, Holota, Hélène, Imbert, Jean, Wolgemuth, Debra J, Gérard, Matthieu, Rousseaux, Sophie, and Khochbin, Saadi

Subjects
Male, Have You Seen...?, Genome, Gene Expression Profiling, Nuclear Proteins, chemical and pharmacologic phenomena, hemic and immune systems, Spermatozoa, Article, Histones, Meiosis, Mice, Animals, Spermatogenesis, Histone Acetyltransferases
Abstract

Male germ cell differentiation is a highly regulated multistep process initiated by the commitment of progenitor cells into meiosis and characterized by major chromatin reorganizations in haploid spermatids. We report here that a single member of the double bromodomain BET factors, Brdt, is a master regulator of both meiotic divisions and post-meiotic genome repackaging. Upon its activation at the onset of meiosis, Brdt drives and determines the developmental timing of a testis-specific gene expression program. In meiotic and post-meiotic cells, Brdt initiates a genuine histone acetylation-guided programming of the genome by activating essential genes and repressing a 'progenitor cells' gene expression program. At post-meiotic stages, a global chromatin hyperacetylation gives the signal for Brdt's first bromodomain to direct the genome-wide replacement of histones by transition proteins. Brdt is therefore a unique and essential regulator of male germ cell differentiation, which, by using various domains in a developmentally controlled manner, first drives a specific spermatogenic gene expression program, and later controls the tight packaging of the male genome.

Published
2012

16. Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells

Author

Morozumi, Yuichi, primary, Boussouar, Fayçal, additional, Tan, Minjia, additional, Chaikuad, Apirat, additional, Jamshidikia, Mahya, additional, Colak, Gozde, additional, He, Huang, additional, Nie, Litong, additional, Petosa, Carlo, additional, de Dieuleveult, Maud, additional, Curtet, Sandrine, additional, Vitte, Anne-Laure, additional, Rabatel, Clothilde, additional, Debernardi, Alexandra, additional, Cosset, François-Loïc, additional, Verhoeyen, Els, additional, Emadali, Anouk, additional, Schweifer, Norbert, additional, Gianni, Davide, additional, Gut, Marta, additional, Guardiola, Philippe, additional, Rousseaux, Sophie, additional, Gérard, Matthieu, additional, Knapp, Stefan, additional, Zhao, Yingming, additional, and Khochbin, Saadi, additional

Published
2015
Full Text
View/download PDF

19. Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B

Author

Montellier, Emilie, primary, Boussouar, Fayçal, additional, Rousseaux, Sophie, additional, Zhang, Kai, additional, Buchou, Thierry, additional, Fenaille, François, additional, Shiota, Hitoshi, additional, Debernardi, Alexandra, additional, Héry, Patrick, additional, Curtet, Sandrine, additional, Jamshidikia, Mahya, additional, Barral, Sophie, additional, Holota, Hélène, additional, Bergon, Aurélie, additional, Lopez, Fabrice, additional, Guardiola, Philippe, additional, Pernet, Karin, additional, Imbert, Jean, additional, Petosa, Carlo, additional, Tan, Minjia, additional, Zhao, Yingming, additional, Gérard, Matthieu, additional, and Khochbin, Saadi, additional

Published
2013
Full Text
View/download PDF

20. Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells.

Author

Yuichi Morozumi, Boussouar, Fayçal, Minjia Tan, Chaikuad, Apirat, Jamshidikia, Mahya, Colak, Gozde, Huang He, Litong Nie, Petosa, Carlo, de Dieuleveult, Maud, Curtet, Sandrine, Vitte, Anne-Laure, Rabatel, Clothilde, Debernardi, Alexandra, Cosset, François-Loïc, Verhoeyen, Els, Emadali, Anouk, Schweifer, Norbert, Gianni, Davide, and Gut, Marta

Abstract

Although the conserved AAA ATPase and bromodomain factor, ATAD2, has been described as a transcriptional co-activator upregulated in many cancers, its function remains poorly understood. Here, usinga combination of ChIP-seq, ChIP-proteomics, and RNA-seq experiments in embryonic stem cells whereAtad2 is normally highly expressed, we found that Atad2 is an abundant nucleosome-bound protein present on active genes, associated with chromatin remodelling, DNA replication, and DNA repair factors. A structural analysis of its bromodomain and subsequent investigations demonstrate that histone acetylation guides ATAD2 to chromatin, resulting in an overall increase of chromatin accessibility and histone dynamics, which is required for the proper activity of the highly expressed gene fraction of the genome. While in exponentially growing cells Atad2 appears dispensable for cell growth, in differentiating ES cells At ad 2 becomes critical in sustaining specific gene expression programmes, controlling proliferation and differentiation. Altogether, this work defines Atad2 as a facilitator of general chromatin-templated activities such as transcription. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

21. Genomic binding of Pol III transcription machinery and relationship with TFIIS transcription factor distribution in mouse embryonic stem cells

Author

Carrière, Lucie, primary, Graziani, Sébastien, additional, Alibert, Olivier, additional, Ghavi-Helm, Yad, additional, Boussouar, Fayçal, additional, Humbertclaude, Hélène, additional, Jounier, Sylvie, additional, Aude, Jean-Christophe, additional, Keime, Céline, additional, Murvai, Janos, additional, Foglio, Mario, additional, Gut, Marta, additional, Gut, Ivo, additional, Lathrop, Mark, additional, Soutourina, Julie, additional, Gérard, Matthieu, additional, and Werner, Michel, additional

Published
2011
Full Text
View/download PDF

33. The CREB coactivator TORC2 is a key regulator of fasting glucose metabolism.

Author

Seung-Hoi Koo, Flechner, Lawrence, Ling Qi, Xinmin Zhang, Screaton, Robert A., Jeffries, Shawn, Hedrick, Susan, Wu Xu, Boussouar, Fayçal, Brindle, Paul, Takemori, Hiroshi, and Montminy, Marc

Subjects
INSULIN, GLUCAGON, HOMEOSTASIS, GENETIC transcription, ADENOSINE monophosphate, CARRIER proteins, PHOSPHORYLATION
Abstract

Glucose homeostasis is regulated systemically by hormones such as insulin and glucagon, and at the cellular level by energy status. Glucagon enhances glucose output from the liver during fasting by stimulating the transcription of gluconeogenic genes via the cyclic AMP-inducible factor CREB (CRE binding protein). When cellular ATP levels are low, however, the energy-sensing kinase AMPK inhibits hepatic gluconeogenesis through an unknown mechanism. Here we show that hormonal and energy-sensing pathways converge on the coactivator TORC2 (transducer of regulated CREB activity 2) to modulate glucose output. Sequestered in the cytoplasm under feeding conditions, TORC2 is dephosphorylated and transported to the nucleus where it enhances CREB-dependent transcription in response to fasting stimuli. Conversely, signals that activate AMPK attenuate the gluconeogenic programme by promoting TORC2 phosphorylation and blocking its nuclear accumulation. Individuals with type 2 diabetes often exhibit fasting hyperglycaemia due to elevated gluconeogenesis; compounds that enhance TORC2 phosphorylation may offer therapeutic benefits in this setting. [ABSTRACT FROM AUTHOR]

Published
2005
Full Text
View/download PDF

34. Histone Acetyltransferase CBP Is Vital To Demarcate Conventional and Innate CD8+ T-Cell Development.

Author

Fukuyama, Tomofusa, Kasper, Lawryn H., Boussouar, Fayçal, Jeevan, Trushar, van Deursen, Jan, and Brindle, Paul K.

Subjects
HISTONES, ACETYLTRANSFERASES, CHROMATIN, T cells, LYMPHOCYTES, INTERLEUKIN-2, GENETIC transcription
Abstract

Defining the chromatin modifications and transcriptional mechanisms that direct the development of different T-cell lineages is a major challenge in immunology. The transcriptional coactivators CREB binding protein (CBP) and the closely related p300, which comprise the KAT3 family of histone/protein lysine acetyltransferases, interact with over 50 T-lymphocyte-essential transcriptional regulators. We show here that CBP, but not p300, modulates the thymic development of conventional adaptive T cells versus those having unconventional innate functions. Conditional inactivation of CBP in the thymus yielded CD8 single-positive (SP) thymocytes with an effector-, memory-, or innate-like T-cell phenotype. In this regard, CD8 SP thymocytes in CBP mutant mice were phenotypically similar to those reported for Itk and Rlk protein tyrosine kinase mutants, including the increased expression of the T-cell master regulatory transcription factor eomesodermin (Eomes) and the interleukin-2 and -15 receptor beta chain (CD122) and an enhanced ability to rapidly produce gamma interferon. CBP was required for the expression of the Itk-dependent genes Egr2, Egr3, and Il2, suggesting that CBP helps mediate Itk-responsive transcription. CBP therefore defines a nuclear component of the signaling pathways that demarcate the development of innate and adaptive naïve CD8+ T cells in the thymus. [ABSTRACT FROM AUTHOR]

Published
2009
Full Text
View/download PDF

35. Loss of CBP causes T cell lymphomagenesis in synergy with p27Kip1 insufficiency

Author

Kang-Decker, Ningling, Tong, Caili, Boussouar, Fayçal, Baker, Darren J., Xu, Wu, Leontovich, Alexey A., Taylor, William R., Brindle, Paul K., and van Deursen, Jan M.A.

Subjects
*TUMOR suppressor genes, *CARCINOGENESIS, *CYCLINS, *PROTEIN metabolism, *PROTEOLYTIC enzymes, *MICE
Abstract

CBP can function as a tumor suppressor, but the mechanisms that govern oncogenesis in its absence are unknown. Here we show that CBP inactivation in mouse thymocytes leads to lymphoma. Although CBP has been implicated in the transactivation functions of p53, development of these tumors does not seem to involve loss of p53 activity. CBP-null tumors show reduced levels of p27Kip1 and increased levels of cyclin E and Skp2, two oncoproteins that can promote p27Kip1 proteolysis. Reduction of p27Kip1 by introduction of a p27Kip1-null allele into CBP knockout mice accelerates lymphomagenesis and seems to obviate the requirement for Skp2 and cyclin E upregulation. These data suggest that CBP loss mediates lymphomagenesis in cooperation with a mechanism that reduces p27Kip1 abundance. [Copyright &y& Elsevier]

Published
2004
Full Text
View/download PDF

36. Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells.

Author

Morozumi Y, Boussouar F, Tan M, Chaikuad A, Jamshidikia M, Colak G, He H, Nie L, Petosa C, de Dieuleveult M, Curtet S, Vitte AL, Rabatel C, Debernardi A, Cosset FL, Verhoeyen E, Emadali A, Schweifer N, Gianni D, Gut M, Guardiola P, Rousseaux S, Gérard M, Knapp S, Zhao Y, and Khochbin S

Subjects
ATPases Associated with Diverse Cellular Activities, Acetylation, Cell Differentiation, Cell Proliferation, Chromatin Immunoprecipitation, Embryonic Stem Cells cytology, Genome, Germ Cells metabolism, Humans, Male, Nucleosomes metabolism, Protein Binding, Proteomics, Adenosine Triphosphatases metabolism, Chromatin metabolism, DNA-Binding Proteins metabolism, Embryonic Stem Cells metabolism
Abstract

Although the conserved AAA ATPase and bromodomain factor, ATAD2, has been described as a transcriptional co-activator upregulated in many cancers, its function remains poorly understood. Here, using a combination of ChIP-seq, ChIP-proteomics, and RNA-seq experiments in embryonic stem cells where Atad2 is normally highly expressed, we found that Atad2 is an abundant nucleosome-bound protein present on active genes, associated with chromatin remodelling, DNA replication, and DNA repair factors. A structural analysis of its bromodomain and subsequent investigations demonstrate that histone acetylation guides ATAD2 to chromatin, resulting in an overall increase of chromatin accessibility and histone dynamics, which is required for the proper activity of the highly expressed gene fraction of the genome. While in exponentially growing cells Atad2 appears dispensable for cell growth, in differentiating ES cells Atad2 becomes critical in sustaining specific gene expression programmes, controlling proliferation and differentiation. Altogether, this work defines Atad2 as a facilitator of general chromatin-templated activities such as transcription., (© The Author (2015). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS.)

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results