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1. Follicular Fluid Growth Factors and Interleukin Profiling as Potential Predictors of IVF Outcomes

Author

Bouricha Molka, Bourdenet Gwladys, Bosquet Dorian, Moussot Lucie, Benkhalifa Mustapha, Cabry Rosalie, Gubler Brigitte, Khorsi-Cauet Hafida, and Benkhalifa Moncef

Subjects
growth factors, interleukins, oocyte quality, repeated implantation failure, cumulative pregnancy rate, Physiology, QP1-981
Abstract

Growth hormone (GH) has gained attention as an anti-aging compound enhancing oocyte quality. In fact, GH is known to activate intrafollicular metabolic events for oocyte maturation. Insulin growth factor I (IGF1) is another ovarian growth factor that mediates the FSH and GH actions. Cytokines could also increase IVF outcomes. Indeed, IL-6 is a pleiotropic cytokine with multiple cellular effects that can vary based on the physiological environment. IL-6 may also play an important role in follicular development (Yang et al., J Assist Reprod Genet, 2020, 37 (5), 1171–1176). Clinical studies have been performed to explore the potential role of IL-6 in human oocyte maturation and subsequent embryonic development. To date, the answers are not conclusive. During peri-implantation, many cytokines balances are regulated like pro-inflammatory and anti-inflammatory interleukins. The pro-inflammatory properties of IL-17 and its impact on the tumor microenvironment or autoimmune diseases are characterized, but new dimensions of IL-17 activity that promotes embryo implantation are not well explored. In the search for answers, our study compared concentrations of growth factors IGF1, GH, and interleukins IL-6 and IL-17 in the follicular fluid (FF) from 140 women divided into two groups depending on bad (G1) or good prognosis (G2) and investigated the relationships between these FF components’ levels and the main parameters of IVF. GH, IGF1, and IL-6 were significantly higher for G2. For GH, it was negatively correlated to patient age and positively correlated to maturity rate and IGF1. Moreover, GH and IGF1 were correlated to the top embryo rate and cumulative pregnancy rate. Regarding IL-6, it was correlated to IGF1 level, endometrium thickness, and implantation rate. As for IL-17, it was only correlated to IL-6. Consequently, all these FF components were predictive of oocyte quality except IL-17. GH seemed to be the best biomarker of this quality.

Published
2022
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2. Endocrine Disruptors and Cancers in Women

Author

Scharschmidt, Lea, Scheffler, Florence, Vandecandelaere, Albane, Bosquet, Dorian, Lefranc, Elodie, Bouquet De La Jolinière, Jean, Benkhalifa, Moncef, Feki, Anis, Cabry-Goubet, Rosalie, and Marci, Roberto, editor

Published
2023
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3. De novo complex intra chromosomal rearrangement after ICSI: characterisation by BACs micro array-CGH

Author

Quimsiyeh Mazin, Aboura Azeddine, Lohmann Laurence, Kasakyan Serdar, Menezo Yves, Tachdjian Gerard, and Benkhalifa Moncef

Subjects
Genetics, QH426-470
Abstract

Abstract Background In routine Assisted Reproductive Technology (ART) men with severe oligozoospermia or azoospermia should be informed about the risk of de novo congenital or chromosomal abnormalities in ICSI program. Also the benefits of preimplantation or prenatal genetic diagnosis practice need to be explained to the couple. Methods From a routine ICSI attempt, using ejaculated sperm from male with severe oligozoospermia and having normal karyotype, a 30 years old pregnant woman was referred to prenatal diagnosis in the 17th week for bichorionic biamniotic twin gestation. Amniocentesis was performed because of the detection of an increased foetal nuchal translucency for one of the fetus by the sonographic examination during the 12th week of gestation (WG). Chromosome and DNA studies of the fetus were realized on cultured amniocytes Results Conventional, molecular cytogenetic and microarray CGH experiments allowed us to conclude that the fetus had a de novo pericentromeric inversion associated with a duplication of the 9p22.1-p24 chromosomal region, 46,XY,invdup(9)(p22.1p24) [arrCGH 9p22.1p24 (RP11-130C19 → RP11-87O1)x3]. As containing the critical 9p22 region, our case is in coincidence with the general phenotype features of the partial trisomy 9p syndrome with major growth retardation, microcephaly and microretrognathia. Conclusion This de novo complex chromosome rearrangement illustrates the possible risk of chromosome or gene defects in ICSI program and the contribution of array-CGH for mapping rapidly de novo chromosomal imbalance.

Published
2008
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4. Genome profiling of ovarian adenocarcinomas using pangenomic BACs microarray comparative genomic hybridization

Author

Qumsiyeh Mazin, Fiorentino Francesco, Baldi Marina, Benkhalifa Moncef, Caserta Donatella, and Moscarini Massimo

Subjects
Genetics, QH426-470
Abstract

Abstract Background Routine cytogenetic investigations for ovarian cancers are limited by culture failure and poor growth of cancer cells compared to normal cells. Fluorescence in situ Hybridization (FISH) application or classical comparative genome hybridization techniques are also have their own limitations in detecting genome imbalance especially for small changes that are not known ahead of time and for which FISH probes could not be thus designed. Methods We applied microarray comparative genomic hybridization (A-CGH) using one mega base BAC arrays to investigate chromosomal disorders in ovarian adenocarcinoma in patients with familial history. Results Our data on 10 cases of ovarian cancer revealed losses of 6q (4 cases mainly mosaic loss), 9p (4 cases), 10q (3 cases), 21q (3 cases), 22q (4 cases) with association to a monosomy X and gains of 8q and 9q (occurring together in 8 cases) and gain of 12p. There were other abnormalities such as loss of 17p that were noted in two profiles of the studied cases. Total or mosaic segmental gain of 2p, 3q, 4q, 7q and 13q were also observed. Seven of 10 patients were investigated by FISH to control array CGH results. The FISH data showed a concordance between the 2 methods. Conclusion The data suggest that A-CGH detects unique and common abnormalities with certain exceptions such as tetraploidy and balanced translocation, which may lead to understanding progression of genetic changes as well as aid in early diagnosis and have an impact on therapy and prognosis.

Published
2008
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9. Effect of Micronutrients and L-Carnitine as Antioxidant on Sperm Parameters, Genome Integrity, and ICSI Outcomes: Randomized, Double-Blind, and Placebo-Controlled Clinical Trial

Author

Lahimer, Marwa, primary, Gherissi, Oumaima, additional, Ben Salem, Nesrine, additional, Ben Mustapha, Henda, additional, Bach, Véronique, additional, Khorsi-Cauet, Hafida, additional, Khairi, Hedi, additional, Ben Ali, Habib, additional, BenKhalifa, Moncef, additional, and Ajina, Mounir, additional

Published
2023
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14. Intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: An RCT

Author

Farifteh, Fattaneh, primary, Fazeli, Elham, additional, Hosseini, Seyedeh Zeinab, additional, Aref, Seyedeh Soheila, additional, Moini, Ashraf, additional, Taheripanah, Robabeh, additional, Rouhollah, Fatemeh, additional, Salehi, Mohammad, additional, Hosseini, Ahmad, additional, and Benkhalifa, Moncef, additional

Published
2023
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16. A no-stop mutation in MAGEB4 is a possible cause of rare X-linked azoospermia and oligozoospermia in a consanguineous Turkish family

Author

Okutman, Ozlem, Muller, Jean, Skory, Valerie, Garnier, Jean Marie, Gaucherot, Angeline, Baert, Yoni, Lamour, Valérie, Serdarogullari, Munevver, Gultomruk, Meral, Röpke, Albrecht, Kliesch, Sabine, Herbepin, Viviana, Aknin, Isabelle, Benkhalifa, Moncef, Teletin, Marius, Bakircioglu, Emre, Goossens, Ellen, Charlet-Berguerand, Nicolas, Bahceci, Mustafa, Tüttelmann, Frank, and Viville, STéphane

Published
2017
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21. Chronic Perigestational Exposure to Chlorpyrifos Induces Perturbations in Gut Bacteria and Glucose and Lipid Markers in Female Rats and Their Offspring

Author

Djekkoun, Narimane, primary, Depeint, Flore, additional, Guibourdenche, Marion, additional, El Khayat El Sabbouri, Hiba, additional, Corona, Aurélie, additional, Rhazi, Larbi, additional, Gay-Queheillard, Jerome, additional, Rouabah, Leila, additional, Hamdad, Farida, additional, Bach, Véronique, additional, Benkhalifa, Moncef, additional, and Khorsi-Cauet, Hafida, additional

Published
2022
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37. Follicular fluid and supernatant from cultured cumulus-granulosa cells improve in vitro maturation in patients with polycystic ovarian syndrome

Author

Madkour, Aicha, primary, Bouamoud, Nouzha, additional, Kaarouch, Ismail, additional, Louanjli, Noureddine, additional, Saadani, Brahim, additional, Assou, Said, additional, Aboulmaouahib, Smahane, additional, Sefrioui, Omar, additional, Amzazi, Saaid, additional, Copin, Henri, additional, and Benkhalifa, Moncef, additional

Published
2018
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38. Embryotoxicity and fetotoxicity following intraperitoneal administrations of hexavalent chromium to pregnant rats

Author

Marouani, Neila, Tebourbi, Olfa, Mokni, Moncef, Yacoubi, Mohamed Tahar, Sakly, Mohsen, Benkhalifa, Moncef, Ben Rhouma, Khémais, Marouani, Neila, Tebourbi, Olfa, Mokni, Moncef, Yacoubi, Mohamed Tahar, Sakly, Mohsen, Benkhalifa, Moncef, and Ben Rhouma, Khémais

Abstract

Heavy metals are omnipresent in the environment, and industrial use has greatly increased their presence in soil, water and air. Their inevitable transfer to the human food chain remains an important environmental issue as many heavy metals cause a range of toxic effects, including developmental toxicity. Administration of chromium VI (1 and 2 mg/kg as potassium dichromate) through intraperitoneal (i.p.) injection during organogenesis (days 6 to 15 of gestation) in rats revealed embryo- and fetotoxic effects. Reduced fetal weight, retarded fetal development, number of fetuses per mother and high incidences of dead fetuses and resorptions in treated mothers were also observed. Gross morphological abnormalities, such as displayed form of edema, facial defect, lack of tail, hypotrophy, severs subdermal haemorrhage patches and hypotrophy of placenta were observed in fetuses after chromium VI-treated mothers. A skeletal development of fetuses presented an incomplete ossification in nasal, cranium, abdominal or caudal bones in rats treated with 1 mg/kg of chromium, whereas rats treated with 2 mg/kg showed ossification and absence of the sacral vertebrae compared with the control. At a higher dose of chromium, histological changes were found in fetuses with atrophy of theirs vital organs. Placental histological observations revealed a pronounced morphological alteration, with atrophy of decidual cells, a degenerated of chorionic villi and hypertrophy of blood lacuna. The present study suggests a risk to the developing embryo when the mother is exposed to a high concentration of chromium VI during organogenesis

Published
2017

42. Effect of Gonadotropin Types and Indications on Homologous Intrauterine Insemination Success: A Study from 1251 Cycles and a Review of the Literature

Author

Cabry-Goubet, Rosalie, primary, Scheffler, Florence, additional, Belhadri-Mansouri, Naima, additional, Belloc, Stephanie, additional, Lourdel, Emmanuelle, additional, Devaux, Aviva, additional, Chahine, Hickmat, additional, De Mouzon, Jacques, additional, Copin, Henri, additional, and Benkhalifa, Moncef, additional

Published
2017
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44. Exome sequencing reveals a nonsense mutation inTEX15causing spermatogenic failure in a Turkish family

Author

Okutman, Ozlem, primary, Muller, Jean, additional, Baert, Yoni, additional, Serdarogullari, Munevver, additional, Gultomruk, Meral, additional, Piton, Amélie, additional, Rombaut, Charlotte, additional, Benkhalifa, Moncef, additional, Teletin, Marius, additional, Skory, Valerie, additional, Bakircioglu, Emre, additional, Goossens, Ellen, additional, Bahceci, Mustafa, additional, and Viville, Stéphane, additional

Published
2015
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47. p,p'-DDT induces testicular oxidative stressinduced apoptosis in adult rats.

Author

Marouani, Neila, Hallegue, Dorsaf, Sakly, Mohsen, Benkhalifa, Moncef, Rhouma, Khémais Ben, and Tebourbi, Olfa

Subjects
RATS, INTRAPERITONEAL injections, LIPID peroxidation (Biology), ANTIOXIDANTS, SUPEROXIDE dismutase
Abstract

Background: The 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p'-DDT) is a known persistent organic pollutant and male reproductive toxicant. The present study is designed to test the hypothesis that oxidative stress mediates p,p'-DDT-induced apoptosis in testis. Methods: Male Wistar rats received an intraperitoneal (ip) injection of the pesticide at doses of 50 and 100mg/kg for 10 consecutive days. The oxidative stress was evaluated by biomarkers such lipid peroxidation (LPO) and metallothioneins (MTs) levels. Antioxidant enzymes activities was assessed by determination of superoxide dismutase (SOD), catalase (CAT) and hydrogen peroxide (H2O2) production. In addition, glutathione-dependent enzymes and reducing power in testis was evaluated by glutathione peroxidase (Gpx), glutathione reductase (GR), glutathione S-transferase (GST) activities and reduced and oxidized glutathione (GSH - GSSG) levels. Apoptosis was evaluated by DNA fragmentation detected by agarose gel electrophoresis. Germinal cells apoptosis and the apoptotic index was assessed through the TUNEL assay. Results: After 10 days of treatment, an increase in LPO level and H2O2 production occurred, while MTs level, SOD and CAT activities were decreased. Also, the Gpx, GR, GST, and GSH activities were decreased, whereas GSSG activity was increased. Testicular tissues of treated rats showed pronounced degradation of the DNA into oligonucleotides as seen in the typical electrophoretic DNA ladder pattern. Intense apoptosis was observed in germinal cells of DDT-exposed rats. In addition, the apoptotic index was significantly increased in testis of DDT-treated rats. Conclusions: These results clearly suggest that DDT sub-acute treatment causes oxidative stress in rat testis leading to apoptosis. [ABSTRACT FROM AUTHOR]

Published
2017
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