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34 results on '"Bauer, J.W."'

2. Cytokeratin 8 interacts with clumping factor B: a new possible virulence factor target

Author

Haim, M., Trost, A., Maier, C.J., Achatz, G., Feichtner, S., Hintner, H., Bauer, J.W., and Onder, K.

Subjects
Staphylococcus aureus -- Physiological aspects, Keratin -- Properties, Virulence (Microbiology) -- Research, Biological sciences
Abstract

Staphylococcus aureus is a human pathogen of growing clinical significance, owing to its increasing levels of resistance to most antibiotics. Infections range from mild wound infections to severe infections such as endocarditis, osteomyelitis and septic shock. Adherence of S. aureus to human host cells is an important step, leading to colonization and infection. Adherence is mediated by a multiplicity of proteins expressed on the bacterial surface, including clumping factor B. In this study, we aimed to identify new targets of clumping factor B in human keratinocytes by undertaking a genome-wide yeast two-hybrid screen of a human keratinocyte cDNA library. We show that clumping factor B is capable of binding cytokeratin 8 (CK8), a type II cytokeratin. Using a domain-mapping strategy we identified amino acids 437-464 as necessary for this interaction. Recombinantly expressed fragments of both proteins were used in pull-down experiments and confirmed the yeast two-hybrid studies. Analysis with S. aureus strain Newman deficient in clumping factor B showed the clumping factor B-dependence of the interaction with CK8. We postulate that the clumping factor B-CK8 interaction is a novel factor in S. aureus infections. DOI 10.1099/mic.0.034413-0

Published
2010

5. 185 CRISPR/Cas9 mediated gene correction of COL7A1

Author

Hainzl, S., primary, Peking, P., additional, Kocher, T., additional, Murauer, E.M., additional, Larcher, F., additional, Río, M del, additional, Duarte, B.G., additional, Steiner, M., additional, Klausegger, A., additional, Bauer, J.W., additional, Reichelt, J., additional, and Koller, U., additional

Published
2017
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23. miR-17, miR-19b, miR-20a and miR-106a are down-regulated in human aging

Author

Strasser, A., Trost, A., Grubeck-Loebenstein, B., Mück, C., Papak, C., Schreiner, C., Herndler-Brandstetter, D., Tschachler, E., Laschober, G., Lepperdinger, G., Kühnel, H., Grillari, J., Bauer, J.W., Fortschegger, K., Eckhard, L., Micutkova, L., Breitenbach, M., Hackl, M., Mildner, M., Rinnerthaler, M., Scheideler, M., Wieser, M., Sampson, N., Berger, P., Jansen-Dürr, P., Grillari-Voglauer, R., Brunner, S., and Trajanoski, Z.

Subjects
Biological Sciences
Abstract

Aging is a multifactorial process where deterioration of body functions is driven by stochastic damage while counteracted by distinct genetically encoded repair systems. In order to better understand the genetic component of aging, many studies have addressed the gene and protein expression profiles of various aging model systems engaging different organisms from yeast to human. The recently identified small non-coding miRNAs are potent post-transcriptional regulators that can modify the expression of up to several hundred target genes per single miRNA, similar to transcription factors. Increasing evidence shows that miRNAs contribute to the regulation of most if not all important physiological processes, including aging. However, so far the contribution of miRNAs to age-related and senescence-related changes in gene expression remains elusive. To address this question, we have selected 4 replicative cell aging models including endothelial cells, replicated CD8+ 13 T cells, renal proximal tubular epithelial cells, and skin fibroblasts. Further included were three organismal aging models including foreskin, mesenchymal stem cells and CD8+ 16 T cell populations from old and young donors. Using LNA-based miRNA microarrays we identified four commonly regulated miRNAs, miR-17 down-regulated in all 7, miR-19b and miR-20a, down-regulated in 6 models and miR-106a down19 regulated in 5 models. Decrease in these miRNAs correlated with increased transcript levels of some established target genes, especially the cdk inhibitor p21/CDKN1A. These results establish miRNAs as novel markers of cell aging in humans.

Published
2009

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