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Author(s): Mutaz E. Salih [sup.1] [sup.2], Ahmad Aqel [sup.3], Babiker Y. Abdulkhair [sup.1] [sup.4], Munir S. Obbed [sup.3], Zeid A. ALOthman [sup.3], Yacine Badjah-Hadj-Ahmed [sup.3], Mohamad A. Abdulaziz [sup.1] Author [...], A home-made column (3.2 mm i.d., 100 mm length) was prepared in which glycidyl polymethacrylate was used. The optimum value of the initiator corresponded to 5 mg/mL. The column morphology was characterized by scanning electron microscopy. The permeability was evaluated using acetonitrile and water as a mobile phase and uracil as an unretained substrate. A simple and economical reversed-phase HPLC method has been developed for the simultaneous estimation of paracetamol and chlorzoxazone in their pharmaceutical formulations. Components were determined using a UV detector at 270 nm. The mobile phase was composed of 1% formic acid solution and acetonitrile (65/35, v/v); 0.7 mL/min flow rate and 5.0 [micro]L injection volume were used. Peak resolution was 1.96. All findings allow concluding that the prepared stainless steel conventional HPLC column and the novel validated method are applicable for quality control and routine analysis.

Arson is a dangerous crime; even a small amount of an ignitable liquid can cause considerable losses in property and even human life. According to the National Fire Protection Association, [...], The identification of ignitable liquids is very important and challenging aspect in arson crime investigations. The detection of gasoline and diesel fuel components using solid phase micro-extraction prior to gas chromatography-mass spectrometry for the forensic analysis of fire debris has been carried out. Previous works show that the absorption characteristics of the substrate are one of the most important factors in determining the evaporation rate of the accelerants. In order to determine the presence of the fuel residues, four of the most common substrate materials were tested in this work; wool, cotton, silk and polyester. The obtained results indicate that both gasoline and diesel fuel accelerants persisted longer on wool and silk than on the other selected substrates. Such information illustrates the influence of fuel persistence times after extinguishing and the best materials to be scanned for ignitable liquids at the fire scene. Keywords: arson, gasoline, diesel, gas chromatography-mass spectrometry, solid phase micro-extraction, SPME