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"Objetivo: evaluar el impacto de la disminución del valor de normalidad de la glucemia en ayuno pro- puesta por la Asociación Americana de Diabetes en 2003 (ADA-2003) sobre la prevalencia de glu- cosa en ayuno alterada (GAA), y la concordancia entre GAA según el criterio ADA-2003 e intoleran- cia a la glucosa, en una población con sospecha diagnóstica de diabetes. Métodos: análisis retrospectivo de los resultados de glucosa en ayuno y dos horas poscarga de 75 g de glucosa en 2062 pacientes con riesgo para diabe- tes. Comparación de la prevalencia de GAA con base en los criterios ADA-1997 y ADA-2003. Resultados: la disminución del punto de corte in- crementa la prevalencia de GAA de 17.7 a 41.3 %. Con ADA-2003 se identificó a 55 % de todos los sujetos con intolerancia a la glucosa; con ADA- 1997, a 22.6 %. Kappa pesada mostró concordan- cia diagonal entre GAA (ADA-2003) y la prueba de intolerancia a la glucosa (criterios actuales de la Organización Mundial de la Salud). Conclusiones: la utilización del criterio ADA-2003 para la glucosa en ayuno alterada permite identifi- car mayor proporción de pacientes con intolerancia a la glucosa o diabetes tipo 2."

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Background Although sophisticated methodologies are available, the use of endpoint polymerase chain reaction (PCR) to detect 16S rDNA genes remains a good approach for estimating the incidence and prevalence of specific infections and for monitoring infections. Considering the importance of the early diagnosis of sexually transmitted infections (STIs), the development of a sensitive and affordable method for identifying pathogens in clinical samples is needed. Highly specific and efficient primers for a multiplex polymerase chain reaction (m-PCR) system were designed in silico to detect the 16S rDNA genes of four bacteria that cause genital infections, and the PCR method was developed. Methods The Genosensor Probe Designer (GPD) (version 1.0a) software was initially used to design highly specific and efficient primers for in-house m-PCR. Single-locus PCR reactions were performed and standardised, and then primers for each locus in turn were added individually in subsequent amplifications until m-PCR was achieved. Amplicons of the expected size were obtained from each of the four bacterial gene fragments. Finally, the analytical specificity and limits of detection were tested. Results Because they did not amplify any product from non-STI tested species, the primers were specific. The detection limits for the Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis and Ureaplasma urealyticum primer sets were 5.12 × 105, 3.9 × 103, 61.19 × 106 and 6.37 × 105 copies of a DNA template, respectively. Conclusions The methodology designed and standardised here could be applied satisfactorily for the simultaneous or individual detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis and Ureaplasma urealyticum. This method is at least as efficient as other previously described methods; however, this method is more affordable for low-income countries.

OBJECTIVE—To evaluate whether educational messages regarding oral glucose tolerance test (OGTT) indications in laboratory reports increase the number of OGTTs appropriately requested. RESEARCH DESIGN AND METHODS—The following message was printed on the lab reports of individuals with a fasting plasma glucose (FPG) concentration between 5.5 and 6.9 mmol/l: “A FPG between 5.5 and 6.9 mmol/l is considered abnormal by the American Diabetes Association (impaired fasting glucose). An OGTT is recommended if the patient does not have a diagnosis of diabetes and suffers from conditions associated with an increased risk for having type 2 diabetes (i.e., overweight, high blood pressure, abnormal plasma lipids or family history of diabetes).” The number of educational messages printed was 81,099. RESULTS—The intervention resulted in a significant increase in the number of OGTTs requested, from 78 ± 19 to 268 ± 48 tests per month. It also resulted in a greater proportion of case subjects that had an abnormal OGTT result. CONCLUSIONS—Educational messages in laboratory reports aid in the diagnostic workup of hyperglycemia.