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Objectives: Exposure to high-molecular-weight polycyclic aromatic hydrocarbons (PAHs) may cause cancer in chimney sweeps and creosote-exposed workers, however, knowledge about exposure to low-molecular-weight PAHs in relation to cancer risk is limited. In this study, we aimed to investigate occupational exposure to the low-molecular-weight PAHs phenanthrene and fluorene in relation to different cancer biomarkers. Methods: We recruited 151 chimney sweeps, 19 creosote-exposed workers and 152 unexposed workers (controls), all men. We measured monohydroxylated metabolites of phenanthrene and fluorene in urine using liquid chromatography coupled to tandem mass spectrometry. We measured, in peripheral blood, the cancer biomarkers telomere length and mitochondrial DNA copy number using quantitative PCR; and DNA methylation ofF2RL3andAHRRusing pyrosequencing. Results: Median PAH metabolite concentrations were higher among chimney sweeps (up to 3 times) and creosote-exposed workers (up to 353 times), compared with controls (p<0.001; adjusted for age and smoking). n-ary sumation OH-fluorene (sum of 2-hydroxyfluorene and 3-hydroxyfluorene) showed inverse associations with percentage DNA methylation ofF2RL3andAHRRin chimney sweeps (B (95% CI)=-2.7 (-3.9 to -1.5) forF2RL3_cg03636183, and -7.1 (-9.6 to -4.7) forAHRR_cg05575921: adjusted for age and smoking), but not in creosote-exposed workers. In addition, n-ary sumation OH-fluorene showed a 42% mediation effect on the inverse association between being a chimney sweep and DNA methylation ofAHRRCpG2. Conclusions: Chimney sweeps and creosote-exposed workers were occupationally exposed to low-molecular-weight PAHs. Increasing fluorene exposure, among chimney sweeps, was associated with lower DNA methylation ofF2RL3andAHRR, markers for increased lung cancer risk. These findings warrant further investigation of fluorene exposure and toxicity., Funding Agencies:Medical Training and Research Agreement (ALF grants; Region Örebro län) OLL550721German Research Foundation (DFG)

Polycyclic aromatic hydrocarbons (PAH) are omnipresent environmental pollutants composed of fused benzene rings and mainly produced by incomplete combustion of organic material. PAH exposure has been associated with increased risk of cancer and probably cardiovascular disease (CVD). In one way or another, everyone is exposed to PAH, but the dose and the period of exposure vary between individuals. Workers who remove soot from chimneys (chimney sweeps) are likely exposed to higher levels of PAH compared with the general population. However, whether the current PAH exposure among chimney sweeps leads to disease is not known. The overall aim of this thesis was to evaluate PAH exposure among currently working chimney sweeps as well as explore early biomarkers related to cardiovascular disease (CVD) and cancer. For this purpose, we recruited 151 chimney sweeps and 152 unexposed control individuals, all males from southern Sweden, from whom we collected questionnaires and biological samples. In one of the studies, we additionally used data and biological samples from 19 creosote-exposed workers, i.e. workers who impregnate wood panels with black oily material rich in PAH known as creosote. We found that PAH exposure (measured as PAH metabolites in urine) was up to 7 times higher among chimney sweeps compared with unexposed control workers, and the levels of PAH metabolites were positively associated with diastolic blood pressure. Moreover, we found higher serum concentrations of the classical risk markers for CVD (homocysteine and cholesterol) in chimney sweeps, compared with controls. Further, we found 25 putative CVD-related serum proteins differentially expressed between nonsmoking chimney sweeps and controls, among which follistatin (FS), heat shock protein beta-1 (HSP 27), and pro-interleukin-16 (IL-16) showed positive dose-response relationships with PAH metabolites. Pathway analysis demonstrated that these 25 proteins were mainly involved in inflammatory response a

Some polycyclic aromatic hydrocarbons (PAH) are known carcinogens and workplace PAH exposure may increase the risk of cancer. Monitoring early cancer-related changes can indicate whether the exposure is carcinogenic. Here, we enrolled 151 chimney sweeps, 152 controls, and 19 creosote-exposed male workers from Sweden. We measured urinary PAH metabolites using LC/MS/MS, the cancer-related markers telomere length (TL) and mitochondrial DNA copy number (mtDNAcn) using qPCR, and DNA methylation of lung cancer-related genes F2RL3 and AHRR using pyrosequencing. The median 1-hydroxypyrene (PAH metabolite) concentrations were highest in creosote-exposed workers (8.0 μg/g creatinine) followed by chimney sweeps (0.34 μg/g creatinine) and controls (0.05 μg/g creatinine). TL and mtDNAcn did not differ between study groups. Chimney sweeps and creosote-exposed workers had significantly lower methylation of AHRR CpG site cg05575921 (88.1% and 84.9%, respectively) than controls (90%). Creosote-exposed workers (73.3%), but not chimney sweeps (76.6%) had lower methylation of F2RL3 cg03636183 than controls (76.7%). Linear regression analyses showed that chimney sweeps had lower AHRR cg05575921 methylation (B=-2.04; P<0.057, adjusted for smoking and age) and lower average AHRR methylation (B=-2.05; P<0.035), and non-smoking chimney sweeps had lower average F2RL3 methylation (B=-0.81; P<0.042, adjusted for age) compared with controls. These cancer-related markers were not associated with urinary concentrations of PAH metabolites. In conclusion, although we found no associations with PAH metabolites in urine (short-term exposure), our results suggest dose-response relationship between PAH exposure and DNA hypomethylation of lung cancer-related loci. These findings indicate that further protective measures should be taken to reduce PAH exposure., Funding Agency:Medical Training and Research Agreement (ALF grants; Region Örebro län) OLL-550721

The genetic background may influence methylmercury (MeHg) metabolism and neurotoxicity. ATP binding cassette (ABC) transporters actively transport various xenobiotics across biological membranes.