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12 results on '"Alfarano, Alda"'

9. Recurrence of Bcl-2/IgH polymerase chain reaction positivity following a prolonged molecular remission can be unrelated to the original follicular lymphoma clone

Author

Ladetto, Marco, primary, Mantoan, Barbara, additional, Ricca, Irene, additional, Astolfi, Monica, additional, Drandi, Daniela, additional, Compagno, Mara, additional, Vallet, Sonia, additional, dell'Aquila, Maria, additional, Alfarano, Alda, additional, Rossatto, Paola, additional, Rocci, Alberto, additional, Vitolo, Umberto, additional, Corradini, Paolo, additional, Boccadoro, Mario, additional, and Tarella, Corrado, additional

Published
2003
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10. Prenatal Diagnosis of Fetal Hemoglobin Lepore-Boston Disease on Maternal Peripheral Blood

Author

Camaschella, Clara, Alfarano, Alda, Gottardi, Enrico, Travi, Maurizio, Primignani, Paola, Cappio, Federico Caligaris, and Saglio, Giuseppe

Abstract

Molecular diagnosis of hemoglobin (Hb) Lepore-Boston in the fetus was successfully accomplished using maternal blood as a source for fetal cells in three pregnancies at risk for β-thalassemia/Hb Lepore disease. Taking advantage of the possibility of amplifying Lepore-specific DNA fragments by polymerase chain reaction and of families in which Hb Lepore was inherited by the paternal side, we demonstrated in two cases and excluded in one case the presence of this hemoglobinopathy in the fetus directly on maternal DNA. The diagnosis was concordant with that obtained by traditional approaches in all three cases. Our results unequivocally show that nucleated fetal cells are present in maternal blood during pregnancy, and demonstrate for the first time that prenatal diagnosis of a genetic disease may be feasible without invasive procedures.

Published
1990
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11. AspergillusGalactomannan Enzyme-Linked Immunosorbent Assay Cross-Reactivity Caused by Invasive Geotrichum capitatum

Author

Giacchino, Mareva, Chiapello, Nadia, Bezzio, Stefania, Fagioli, Franca, Saracco, Paola, Alfarano, Alda, Martini, Vincenza, Cimino, Giuseppe, Martino, Pietro, and Girmenia, Corrado

Abstract

ABSTRACTWe report three cases of invasive Geotrichum capitatuminfection in patients with acute leukemia for which an enzyme-linked immunosorbent assay (ELISA) for Aspergillusgalactomannan was positive, with no evidence of aspergillosis. Supernatants obtained from suspensions of 17 G. capitatumstrains gave positive reactions with the Aspergillusgalactomannan ELISA. These clinical and laboratory data seem to suggest that G. capitatumproduces a soluble antigen that is cross-reactive with Aspergillusgalactomannan.

Published
2006
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12. Inter-laboratory comparison of HCV-RNA assay results: implications for multi-centre research

Author

Pembrey, L, Newell, Ml, Tovo, Pier Angelo, van Drimmelen, H, Quinti, I, Furlini, G, Galli, S, Meliconi, Mg, Burns, S, Hallam, N, Sönnerborg, A, Cilla, G, Serrano, E, Laccetti, P, Portella, G, Polywka, S, Icardi, G, Bruzzone, B, Balbo, L, Alfarano, A, Virus Network, European Paediatric Hepatitis C., Fabris, Claudio, Pembrey, Lucy, Newell, Marie-Louise, Tovo, Pier-Angelo, van Drimmelen, Harry, Quinti, Isabella, Furlini, Giuliano, Galli, Silvia, Meliconi, Maria Grazia, Burns, Sheila, Hallam, Nick, Sönnerborg, Ander, Cilla, Gustavo, Serrano, Esther, Laccetti, Paolo, Portella, Giuseppe, Polywka, Susanne, Icardi, Giancarlo, Bruzzone, Bianca, Balbo, Luciano, Alfarano, Alda, European Paediat Hepatitis C Virus [,Lanari Marcello,], Pembrey, EUROPEAN PAEDIATRIC HEPATITIS C. VIRUS N. E. T. W. O. R. K. L., M. L., Newell, P. A., Tovo, H., VAN DRIMMELEN, I., Quinti, G., Furlini, S., Galli, M. G., Meliconi, S., Burn, N., Hallam, A., Snnerborg, G., Cilla, E., Serrano, P., Laccetti, S., Polywka, G., Icardi, B., Bruzzone, L., Balbo, and A., Alfarano

Subjects
hepatitis C virus, Quality Control, Reproducibility of Result, Hepacivirus, Polymerase Chain Reaction, Sensitivity and Specificity, quantitative, international proficiency study, Humans, hepatitis C viru, Multicenter Studies as Topic, False Positive Reactions, Hepaciviru, Reproducibility of Results, Infant, polymerase chain reaction (PCR), qualitative, False Positive Reaction, Hepatitis C, Europe, Child, Preschool, RNA, Viral, Female, Reagent Kits, Diagnostic, Laboratories, Human
Abstract

To investigate whether it is appropriate to assume comparability of hepatitis virus C (HCV)-RNA results across laboratories in multi-centre studies, nine laboratories of the European Paediatric HCV Network participated in an international proficiency study of HCV-RNA assays. A panel of 12 samples of different dilutions and genotypes was sent to each laboratory and tested with qualitative and/or quantitative HCV-RNA assays according to local procedures. Commercial assays were used in seven laboratories and in-house assays in two. All six laboratories in which a commercial qualitative assay was used were proficient, as were four of six runs (in five laboratories) in which a commercial quantitative assay was used. The proficiency of the laboratories where in-house assays were used could not be assessed according to the VQC definition because of differences in the methods used. Overall, there were several false-negative results, but only one false-positive result with a quantitative assay and none with a qualitative assay. The false-negative results may have implications for the diagnosis of infection, and highlight the need for an antibody test to be performed at 18 months to confirm the absence of infection. The results of qualitative assays were generally consistent across laboratories but it was difficult to evaluate and compare the results of quantitative assays. Multivariate analysis of data collected in multi-centre studies should therefore allow for centre and/or assay used.

Published
2004

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