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The structure and composition of Mediterranean biodiversity has changed significantly. So far, in Montenegrin coastal waters, nine new non-native species have been recorded: Tylosurus acus imperialis, Caranx crysos, Siganus luridus, Fistularia commersonii, Stephanolepis diaspros, Sphoeroides pachygaster, Lagocephalus sceleratus, Callinectes sapidus and Farfantepanaeus aztecus. Allochthonous species are starting to compete for food and space and leading to habitat degradation, socio-economic impacts and can hybridise with the native species. Natural factors and human activities have enabled the arrival of non-native species into the Adriatic Sea. Four of the species are Lessepsian immigrants, which arrived via the Suez Canal, but five others were introduced from the Atlantic Ocean, through the Strait of Gibraltar. Analysis using the Marine Fish Invasiveness Screening Kit (MFISK), Aquatic Invasiveness Screening Kit (AS-ISK) and Receiver operating characteristic (ROC) showed a calibration threshold of 22.5 for MFISK, a BRA (Basic Risk Assessment) score of 34 and a CCA (Climate Change Assessment) score of 46. A measure of the accuracy of the calibration analysis is the area under the ROC curve (AUC). Two species were characterised as non-invasive: Tylosurus acus imperialis and Caranx crysos, but five others were characterised as invasive and covered by the categories from moderate to high-risk., Struktura i sastav bioraznolikosti Sredozemlja značajno su se promijenili. Do sada je u crnogor- skom primorju zabilježeno devet novih ne-nativnih vrsta: Tylosurus acus imperialis, Caranx crysos, Siganus luridus, Fistularia commersonii, Stephanolepis diaspros, Sphoeroides pachygaster, Lagocephalus sceleratus, Callinectes sapidus i Farfantepanaeus aztecus. Alohtone vrste počinju se natjecati za hranu i prostor i dovode do degradacije staništa, socio-ekonomskih učinaka i mogu hibridizirati s autohtonim vrstama. Prirodni čimbenici i ljudske aktivnosti omogućili su dolazak ne- nativnih vrsta u Jadransko more. Četiri vrste su lesepsijski imigranti, koji su stigli preko Sueskog kanala, ali pet drugih uneseno je iz Atlantskog oceana, kroz Gibraltarski tjesnac. Analiza korištenjem programa MFISK (Marine Fish Invasiveness Screening Kit), AS-ISK (Aquatic Invasiveness Screen- ing Kit) i ROC (Receiver operating characteristic) pokazala je prag kalibracije od 22,5 za MFISK, BRA (Basic Risk Assessment)) rezultat od 34 i CCA (Climate Change Assessment) ocjenu od 46. Mjera točnosti kalibracijske analize je površina ispod ROC krivulje (AUC). Dvije vrste okarakter- izirane su kao neinvazivne: Tylosurus acus imperialis i Caranx crysos, no pet drugih okarakter- izirano je kao invazivno i obuhvaćeno kategorijama od umjerenog do visokog rizika.

The sandbar shark (Carcharhinus plumbeus) is considered rare in the Adriatic Sea and the majority of records originate from the northern Adriatic, where a nursery area for this species close to the Po delta system has been repeatedly proposed. This study provides 5 new records and analyses the previously published records of sandbar sharks recorded around the delta of the River Bojana (in Montenegro, in the south-eastern Adriatic). The River Bojana located on the border between Montenegro and Albania, is the second largest river flowing into the Adriatic Sea, where it forms a highly productive ecosystem already known as a local hotspot for smooth-hound sharks (Mustelus spp.). New records of sandbar sharks have emerged as a result of citizen science (a social media survey) and direct reports from fishermen. The total length of C. plumbeus juveniles ranged from approximately 800 mm to 1100 mm, and most (n=5) were caught by set gillnets. The data presented here show that juveniles are consistently present around the estuary and indicate the importance of this fragile estuarine ecosystem for sandbar sharks. Additionally, this study also provides morphometric data collected from a single individual.

The blue shark belongs to the group of the most widespread pelagic sharks in the world. It inhabits the pelagic zone of almost all warm and temperate seas and oceans. As it is one of the most abundant shark species, it is frequently caught in different types of fishing gear operated in the pelagic zone. This study provides the first data on the reproduction of this species in Montenegrin waters (in the Southeastern Adriatic Sea). Six newborn blue sharks were found during our field excursions. For three of them, detailed morphometric measurements are presented. Alongside the newborns, a gravid female was also recorded. The data was collected in the period from April to October 2017. According to the published literature and the data presented in this paper, species reproduction is likely to be seasonal. A review of the areas where the specimens were recorded was carried out in order to infer if the adult females choose them specifically as their parturition grounds., Pas modrulj pripada grupi najviše rasprostranjenih pelagičnih morskih pasa na svijetu. Obitava u pelagičnoj zoni gotovo svih umjerenih i toplih mora i oceana. Premda je jedan od najčešćih morskih pasa, često se lovi različitim ribolovnim alatima koji se koriste u pelagijalu. Ovim istraživanjem sakupljeni su prvi podaci o reprodukciji ove vrste u Crnoj Gori (Jugoistočni Jadran). Tijekom terenskih istraživanja, evidentirano je šest tek rođenih jedinki. Za tri jedinke, prikazane su detaljne morfo-metrijske mjere. Pored spomenutih jedinki, zabilježena je i gravidna ženka ove vrste. Svi podaci su sakupljeni u razdoblju od travnja do listopada 2017. Prema do sada objavljenoj literaturi i ovdje prikazanim podacima, moguće je da je reprodukcija ove vrste sezonska. Izvršen je i pregled lokaliteta na kojima su jedinke pronađene, kako bi se utvrdilo biraju li ih ženke kao mjesta za rađanje mladih.

Molluscan shells are an example of a mineral-based biocomposite material, and most studies to date have focused on understanding their biomineralization mechanisms. Meanwhile, large amounts of these shells are produced as waste globally by seafood which is used by other industries as a source of biogenic calcium carbonates. In this study, we propose a simple methodological approach for isolation ofConchixes, the organic scaffolds that resemble the size and shapes of mollusks shells, using gentle EDTA-based demineralization of the shells. Such mineral-free biological materials have been extracted from selected representatives of marine and fresh water bivalves, as well as from marine and terrestrial gastropods under study. Key pathways to practical applications of molluscanconchixeswith regards to pharmacy, cosmetics, feed and feed additives, biomedicine and bioinspired materials science are also discussed.

The annual alteration of gonad morphology in anchovy, Engraulis encrasicolus (Linnaeus,1758), caught in the period from July 2006 to June 2007 using beach seines in the region of Boka Kotorska Bay (Montenegro, southern Adriatic) was studied. Samples were taken each month. Total length of individuals ranged from 6.6 to 12.2 cm, while weight ranged from 1.58 to 11.27 g, Their ovaries were extracted and weighed, and a piece of female gonad tissue was sampled for histological analysis. The length–weight relationship of all anchovy specimens was described by the equation: \(W = 0.0032\) \(L_T^3.2537; (r^2 = 0.9528)\). The lowest GSI values were found in November and remained at low levels until April, which corresponded to the gonad rest stage. The GSI increased gradually during April and May, which coincides with the begining of spawning period. Although primary oocytes (stage I) were present during all months, their percentage increased from October and was the highest during the winter months (November-March 100%). Mature cells, stage IV, were present during April – October period, with highest value in May when they comprised 12.3% of total oocyte number. An increase in the percentage of oocytes in the yolk vesicle (II) and yolk (III) stages occurred in April, remaining almost unchanged until October. Smallest average oocyte size was noticed in period November – February with an average of 59.69 μm, while greatest size was recorded in April 2007, 185.84 μm.

This study examined the relationship between the sagittal otolith morphometric variables (length, height and weight) and body growth of the European eel. Eels that were studied ranged in total length from 11.2 to 79.5 cm. The relationships between the sagittal otolith variables and fish somatic growth were described with a non-linear function. The resulting coefficients of determination ((r^2)) ranged from 0.782 to 0.914. The variable most strongly related to fish size was found to be the sagittal otolith length (OL) with 91.4 % of the variability. The results of this study provide the first comprehensive data regarding the relationship between the sagittal otolith morphometric variables with the body length of Anguilla anguilla., U ovom radu se opisuje odnos između morfometrijskih varijabli sagitalnog otolita (duljina, visina i težina) i rasta tijela europske jegulje. Proučavane jegulje imale su raspon ukupne dužine od 11,2 do 79,5 cm. Odnos između sagitalnih otolitskih varijabli i somatskog rasta ribe opisani su nelinearnom funkcijom. Dobiveni koeficijenti određivanja ((r^2)) kretali su se od 0,782 do 0,914. Varijabla koja je najsnažnije povezana s veličinom riba utvrđena je na temelju sagitalne duljine otolita (OL) s 91,4% varijabilnosti. Rezultati ove studije pružaju prve sveobuhvatne podatke o odnosu između morfometrijskih varijabli sagitalnog otolita sa dužinom tijela Anguilla anguilla.

Due to strengthening of environmental constraints and current industrial competitiveness, the airplane manufacturing industry is urged to turn towards an increase use of sustainable energy sources. A prominent concept is airplane electrification, either of the engine or various non-propulsive systems. In this paper, electrification of the Environmental Control System (ECS), which is used for cabin pressurization and electronic devices cooling, is analyzed. The objective is to develop a calculation method which allows to study the impact of ECS electrification on the aircraft mission performance, by taking into account the ambient air extraction impact on the aircraft drag. The method can be used at early design, for a complete aircraft mission, and is based on penalty analysis methods to convert the system performance impacts into fuel weight delta. In this paper a conventional and a fully electrified architecture are compared for a short-medium range aircraft. While the electrical ECS architecture is shown to be more advantageous with respect to the engine performance alone, preliminary studies using the presented method indicate that a conventional ECS architecture is more adapted regarding the overall aircraft mission fuel performance.

The objective of this paper is development and application of a methodology for preliminary analysis of variable pitch fan (VPF), both as a separate component and as a module integrated into a short-medium range geared turbofan engine developed within European FP7 project ENOVAL. For this purpose, a high bypass ratio two spool geared turbofan engine model was constructed in software PROOSIS. A VPF performance modeling methodology was then developed using 3D steady RANS CFD produced fan maps as baseline; the CFD maps characterized five discrete fan pitch angle settings. In order to represent those maps in PROOSIS and add the pitch angle as a degree of freedom, they were transformed into the Map Fitting Tool (MFT) reference frame. Once the complete VPF turbofan model was in place, engine mission optimization experiments were carried out. The resulting performance is characterized by a good capability to control the fan surge margin, without degrading the engine fuel consumption. This paper represents a new contribution on the topic firstly by coupling a 0D engine performance code with a 3D RANS calculation, and then by introducing the concept of MFT maps with an additional degree of freedom as the interface between the two.

The growth of Mullus barbatus has been widely studied using different methods, but no previous study has focused on age validation. The uncertainty in estimating the age of the red mullet by otolith reading is linked to the number of false-growth increments laid down before the annulus. The capture of red mullets in the early life stage allowed us to estimate their size at the metamorphosis from the pelagic to the demersal phase. The comparison between the metamorphosis size and the back-calculated length of the first growth increment clarified the position of the false growth increment on the otolith. Moreover, the analyses of the otolith marginal increments in adult and juvenile specimens allowed us to define the deposition patterns of their annuli. The modal components of the length–frequency distribution analysis (LFDA) were identified in the winter survey (ELEFAN and Bhattacharya methods), and they did not show significant differences from the length back-calculation of the annuli. Moreover, no significant differences were found between the growth curves calculated by otolith reading (back-calculation and direct otolith reading) and the LFDA. The agreement between the length–frequency results and the otolith age estimation either corroborated or indirectly validated the growth pattern estimated in the otoliths of the red mullet, mainly when the direct validation methods (e.g. mark-recapture, captivity, radiochemical) were difficult to implement, like the case of this species. The comparison of the results of the present work to previous Mediterranean studies showed agreement with the slow growth pattern.

The main aim of the present study is to increase the knowledge on the seasonal dynamics of the set gears used by Adriatic small-scale fisheries (SSFs) through the collation of data (landings, fishing effort, composition of catches, length-frequency distributions of target species and economic value of landings) on target species,. The study focused on the three set gears mainly used in the basin: gillnets, trammel nets and traps. The results confirmed that SSFs are diverse, complex and dynamic, representing an economic and social engine and providing seafood and employment to local economies. In the Adriatic Sea, SSFs exploit coastal fishing grounds where the seasonal fluctuations of the physical and chemical parameters of the water column strongly influence the occurrence of the different species. Because of this, many different types of set gears are currently used to catch a pool of target species over the year. Fishermen use more than one set gear during the year and tend to increase the fishing effort when the target species concentrate inside the coastal areas during the recruitment or the spawning season. Accordingly, landings reached the highest values during such periods and often include a large portion of juveniles and/or spawners. The study highlighted that the lack of appropriate data and complete statistics is still one of the main constraints for most of the Adriatic coastal countries, as few countries have in place routinely monitoring programs while others are dealing mostly with estimates. These gaps often have the effect of marginalizing and undervaluing the real role of SSFs in a Blue Growth process.

El objetivo fue determinar el perfil bioquimico de los equipos juveniles de futbol nacional y comparar los valores de nueve parametros bioquimicos entre tres equipos nacionales serbios (menores de 14, 15 y 16 anos de edad), asi como entre los jugadores de futbol y no atletas. Ochenta jovenes jugadores de futbol y treinta no atletas participaron en el estudio. Se midieron nueve parametros bioquimicos (glucosa, colesterol, trigliceridos, urea, creatinina, bilirrubina total, AST (SGOT), ALT (SGPT), hierro). Con el fin de determinar la significacion de las diferencias entre los grupos en un nivel multivariado se administro un analisis multivariado de varianza (MANOVA), y para poner a prueba las diferencias entre los grupos en un nivel univariado se aplico un analisis univariado de la varianza (ANOVA). Se encontraron diferencias estadisticamente significativas entre los grupos (jugadores de futbol hasta 14, 15 y 16 anos de edad) en un nivel multivariado de las variables bioquimicas (MANOVA, p= 0,00). ANOVA tambien revelo diferencias significativas en creatina (p= 0,00), la bilirrubina total (p= 0,00) y ALT (SGPT) (p= 0,02). Se encontraron diferencias estadisticamente significativas en las variables aplicadas entre los jugadores de futbol y no atletas a nivel multivariado (MANOVA p= 0,00). ANOVA tambien revelo diferencias significativas en glucosa, colesterol, trigliceridos, creatinina, bilirrubina total, ALT (SGPT) y hierro (p= 0,00) entre los jugadores de futbol y no atletas. Sin embargo no hubo diferencias estadisticamente significativas en otras variables (AST (SGOT) y urea). Se concluyo que a excepcion de la AST (SGOT) y urea no existe diferencia significativa en casi todas las variables (glucosa, colesterol, trigliceridos, etc.) entre los jugadores de futbol y no atletas. Desde un punto de vista practico, el medico debe tener en cuenta no solamente la edad, sino tambien la formacion de estado de los individuos en la evaluacion de sus analisis de sangre.

Small-scale fisheries (SSF) in Croatia and Montenegro have a long tradition, similar to those of SSFs in other parts of the coastal Mediterranean. In order to improve fisheries management and save the cultural heritage of fishing traditions on the eastern Adriatic coast, scientists and fisheries managers, from these two countries, made an effort, to gain a better understanding of fishing activities and targeted stocks. This is a complex research topic, due to the high and increasing fishing pressure on marine resources as well as the fact that fisheries management throughout the Mediterranean region remains conventional in its nature. Basic characteristics of SSFs in each country are presented. Specific conclusions related to improvement of coastal resources state in term of sustainable use are suggested together with proposal for additional conservation measures and actively involving fishermen in the management process. Finally, guidelines for future management in terms of monitoring and data collection framework (DCF) are proposed for both countries.

The objective of this paper is development and application of a methodology for preliminary analysis of variable pitch fan (VPF), both as a separate component and as a module integrated into a short-medium range geared turbofan engine developed within European FP7 project ENOVAL. For this purpose, a high bypass ratio two spool geared turbofan engine model was constructed in software PROOSIS. A VPF performance modelling methodology was then developed using 3D steady RANS CFD produced fan maps as baseline; the CFD maps characterised five discrete fan pitch angle settings. In order to represent those maps in PROOSIS and add the pitch angle as a degree of freedom, they were transformed into the Map Fitting Tool (MFT) reference frame. Once the complete VPF turbofan model was in place, engine mission optimisation experiments were carried out. The resulting performance is characterised by a good capability to control the fan surge margin, without degrading the engine fuel consumption.

In this Collective Article on “New Mediterranean Biodiversity Records”, we present additional records of species found in the Mediterranean Sea. These records refer to eight different countries mainly throughout the northern part of the basin, and include 28 species, belonging to five Phyla. The findings per country include the following species: Spain: Callinectes sapidus and Chelidonura fulvipunctata; Monaco: Aplysia dactylomela; Italy: Charybdis (Charybdis) feriata, Carcharodon carcharias, Seriola fasciata, and Siganus rivulatus; Malta: Pomacanthus asfur; Croatia: Lagocephalus sceleratus and Pomadasys incisus; Montenegro: Lagocephalus sceleratus; Greece: Amathia (Zoobotryon) verticillata, Atys macandrewii, Cerithium scabridum, Chama pacifica, Dendostrea cf. folium, Ergalatax junionae, Septifer cumingii, Syphonota geographica, Syrnola fasciata, Oxyu- richthys petersi, Scarus ghobban, Scorpaena maderensis, Solea aegyptiaca and Upeneus pori; Turkey: Lobotes surinamensis, Ruvettus pretiosus and Ophiocten abyssicolum. In the current article, the presence of Taractes rubescens (Jordan & Evermann, 1887) is recorded for the first time in the Mediterranean from Italy. The great contribution of citizen scientists in monitoring biodiversity records is reflected herein, as 10% of the authors are citizen scientists, and contributed 37.5% of the new findings., peer-reviewed

Some aspects of the reproductive biology of females of the deep-water pink shrimp, Parapenaeus longirostris (Lucas, 1846) from the Montenegrin shelf (southern Adriatic Sea) were investigated. Sampling was performed on a monthly basis from November 2006 to October 2007. A total of 13 trawl hauls covered a depth range of 40 to 250 m. An estimate of the gonadosomatic index (GSI) showed permanent reproductive activity all year round with two maxima, one in late spring and the other in autumn. The minimum reproductive activity occurred in June. Females outnumbered males in shallow waters, while at greater depths (> 100 m) the proportion of the sexes was almost equal; the overall sex ratio was 0.7 females : males. The size at which 50% of the female population reached maturity was at a carapace length (CL) of 21.9 mm, and the smallest mature female in the samples had a carapace length of 16 mm.

Red mullet, Mullus barbatus Linnaeus, 1758, is an economically very important species in trawl fisheries in the Adriatic Sea. Material for analysis was collected from October 2002 until May 2004 with a bottom trawl net from commercial trawlers on the Montenegrin shelf. Mortality of this species was analyzed for the first time in this area, sepa?rately for males and females and for both sexes together. Until now, only the spatial distribution and catch per unit of effort (CPUE) of Mullus barbatus have been studied in Montenegrin waters. Estimated total mortality rates were Zmales = 0.653 and Zfemales = 0.712. The average mortality rate for both sexes was Z m+f = 0.749, while the natural mortality rate was Mm+f = 0.342. Values of Z in the Montenegrin shelf area are considerably lower than Z values for the Croatian and Italian parts of the Adriatic Sea, indicating that on the Montenegrin shelf fishing of this species is significantly less intensive than in other trawl-fishing areas of the Adriatic Sea.

SUMMARY This paper presents the results of physical and chemical changes that occur during the process of hot smoking of rainbow trout (Oncorhynchusmykiss), including differences occurring when using fresh or frozen/thawed fish as raw material for the same process. Fresh fish were gutted and subjected to temperature 'shocking' (-35°C for 3.5 h) before salting in a 2% NaCl solution for 12 h. The fish were subsequently smoked at 75-80 °C for 2 h. All material for analysis, 92 fishes, was provided by the private company "AHILEAS", Podgorica (Montenegro), and analysis were made following processing steps used in this company in the process of hot smoking. Water content, water holding capacity, pH and yield were determined before processing, after salting, and after smoking. The possible effect of using frozen/thawed fish as raw material was studied by freezing (-17°C, 30 days) a group of the fresh gutted fish. After slow thawing, the fish were processed similar to the fresh raw material. Results of the performed analysis indicate that freezing of fish has no influence on the quality and yield of final, smoked product. The research was performed as a part of the SEAMED project, financed by the Norwegian ministry of foreign affairs through HERD (High Education and Research for Development) programme for West Balkans.

Increasing human pressures and global environmental change may severely affect the diversity of species assemblages and associated ecosystem services. Despite the recent interest in phylogenetic and functional diversity, our knowledge on large spatio-temporal patterns of demersal fish diversity sampled by trawling remains still incomplete, notably in the Mediterranean Sea, one of the most threatened marine regions of the world. We investigated large spatio-temporal diversity patterns by analysing a dataset of 19,886 hauls from 10 to 800 m depth performed annually during the last two decades by standardised scientific bottom trawl field surveys across the Mediterranean Sea, within the MEDITS program. A multicomponent (eight diversity indices) and multi-scale (local assemblages, biogeographic regions to basins) approach indicates that only the two most traditional components (species richness and evenness) were sufficient to reflect patterns in taxonomic, phylogenetic or functional richness and divergence. We also put into question the use of widely computed indices that allow comparing directly taxonomic, phylogenetic and functional diversity within a unique mathematical framework. In addition, demersal fish assemblages sampled by trawl do not follow a continuous decreasing longitudinal/latitudinal diversity gradients (spatial effects explained up to 70.6% of deviance in regression tree and generalised linear models), for any of the indices and spatial scales analysed. Indeed, at both local and regional scales species richness was relatively high in the Iberian region, Malta, the Eastern Ionian and Aegean seas, meanwhile the Adriatic Sea and Cyprus showed a relatively low level. In contrast, evenness as well as taxonomic, phylogenetic and functional divergences did not show regional hotspots. All studied diversity components remained stable over the last two decades. Overall, our results highlight the need to use complementary diversity indices through different spatial scales when developing conservation strategies and defining delimitations for protected areas., SI

The recent increase of the local population of gilthead seabream, Sparus aurata, in three areas along the southeastern Adriatic Sea: Malostonski Bay (Croatia and Bosnia and Herzegovina), Neretva Estuary (Croatia) and Boka Kotorska Bay (Montenegro) and its adverse effects on shellfish culture by preying on Mediterranean mussels, Mytilus galloprovincialis, and the European flat oyster, Ostrea edulis, are studied. The results from the analysis of the existing information show that the main reason for the recent increase is the escapes from local fish farm which enrich the local population constantly with new gilthead sea bream. The existence of practically endless food in the area of the shellfish farms allows the concentration of the population in the region instead of its dispersion along the Adriatic coast. Moreover, ecological analysis indicates that the gilthead seabream is facing a very low competition from other local species which enhances its capacity to further populate the region. While the impact on the ecosystem is not yet known, the socio-economic impact of the increase of the gilthead seabream population is evident today. Many shellfish farms are closing today in the region since the damages may reach over 90 % of the production.

Daily growth rate of juvenile anchovy, Engraulis encrasicolus (Linnaeus, 1758), was estimated in the south-eastern Adriatic Sea. Samples were taken by a commercial beach seine net and the size range of 100 sampled individuals was 5.0-6.8 cm total length. Age was estimated by counting growth increments of sagittal otoliths, which are formed with daily periodicity. Estimated ages were 41- 90 days and hatch date for each age class was back-calculated from the date of capture. The calculated instantaneous growth rate was 0.41 mm day-1. Fish size at metamorphosis from late larva to juvenile was estimated to 3.2 cm total length. Parameters of the von Bertalanffy’s growth function were L∞ = 10.41 and K = 3.36, Dnevna stopa rasta juvenilnog inćuna, Engraulis encrasicolus (linnaeus, 1758), procijenjena je u jugoistočnom Jadranu. Uzorci su sakupljani komercijalnom obalnom mrežom potegačom, a raspon veličine kod 100 uzorkovanih jedinki iznosio je 5.0-6.8 cm ukupne dužine. dob je procijenjena brojenjem dnevnih naraštajnih prstenova na sagitalnim otolitima. Procijenjena starost kolebala je između 41- 90 dana, a nadnevak izvaljivanja ličinke za svaki starosni razred bio je naknadno izračunat prema datumu ulova. Izračunata trenutna stopa rasta iznosila je 0.41 mm dan -1. Veličina ribe za vrijeme metarmofoze iz kasnog stadija ličinke u juvenilni stadij procijenjena je na 3.2 cm ukupne dužine. Parametri u von Bertalanffy-jevoj jednadžbi rasta bili su L∞ = 10.41 i K = 3.36.

Five anchovy (Engraulis encrasicolus, Linnaeus 1758) eggs were caught during an ichthyoplankton survey in Boka Kotorska Bay (south Adriatic Sea) with a PairOVET (modified CalVet) plankton net on December 5th, 2006. Eggs were found at two sampling stations (42°28’30.24” N, 18°44’41.81” E and 42°29’30.23” N, 18°40’41.84” E). It is the third record of anchovy eggs in the winter months in the Adriatic Sea., Pet jaja brgljuna (Engraulis encrasicolus, Linnaeus 1758) su uhvaćena tijekom istraživanja ihtioplanktona u Bokokotorskom zaljevu (južni Jadran) s PairOVET (izmijenjeni CalVet) i planktonskom mrežom 5. prosinca 2006. godine. Jaja su pronađena na dva mjesta uzorkovanja (42 ° 28’30 .24 “N, 18 ° 44’41 0,81” E i 42 ° 29’30 0,23 “N, 18 ° 40’41 0,84” E). Ovo je treći nalaz jaja brgljuna u zimskim mjesecima u Jadranu.

The annual alteration of gonad morphology in sardine (Sardina pilchardus, Walbaum 1792) caught in the period from November 2006 to October 2007 using beach seines in the region of Boka Kotorska Bay (Montenegro, southern Adriatic) was studied. Samples were taken in the middle of each month. Total length of individuals ranged from 8.7 to 14.7 cm and weight ranged from 4.67 to 22.61 g. Their gonads were extracted and weighed, and a piece of gonad tissue was sampled for histological analysis. The length–weight relationship of all sardine specimens was described by the equation: W=0.0059 LT3.0891; (r2 = 0.963). The lowest gonadosomatic index (GSI) values, below 1, were found in June, July and August, corresponding to the state of gonad rest. The GSI increased gradually from September and October and reached its highest value in February, at 5.22 for females and 6.58 for males. After February the GSI started to decrease throughout March (4.5), April (2.4) and May (2.4). In June all gonads had a GSI bellow 1. Although primary oocytes (stage I) were present during all months, their percentage increased from May and was the highest during the summer months (June-September 100%). Mature stage IV oocytes were recorded from November to April, with the highest percentage recorded during January (26.7%). An increase in the percentage of oocytes in the yolk vesicle (II) and yolk (III) stages occurred in October, remaining almost unchanged until May. During the summer months (May – September), only stage I spermatogonia were present in the testicles. In October, spermatocytes (stage II) started to appear, while from November the spermatids (stage III) appeared as well. Spermatozoa (stage IV) appeared in December and reached their maximum level in January and February., Istraživana je godišnja promjena morfologije gonada kod srdele (Sardina pilchardus Walbaum 1792) ulovljene obalnom mrežom potegačom u razdoblju od studenog 2006. do listopada 2007. na području Bokokotorskog zaljeva (Crna Gora, južni Jadran). Ukupna dužina primjeraka kretala se od 8.7 do 14.7 cm, a težina od 4.67 do 22.61 g. Obavljena je histološka analiza gonada. Dužinsko– težinski odnos za sve jedinke može se opisati jednadžbom: W=0.0059 LT 3.0891 (r2 = 0.963). Najniže vrijednosti gonadosomatskog indeksa (GSI) utvrđene su u lipnju, srpnju i kolovozu, što odgovara stadiju mirovanja gonada. GSI vrijednosti su se postupno ovećavale u rujnu i listopadu te dosegle najviše vrijednosti u veljači 5.22 za ženke, odnosno 6.58 za mužjake. Nakon veljače, GSI vrijednosti su opadale, kroz ožujak (4.5), travanj (2.4) i svibanj (2.4). U lipnju su GSI vrijednosti bile manje od 1. Iako su primarne oocite bile prisutne u svim mjesecima, njihova zastupljenost je počela rasti u svibnju i dosegla najvišu vrijednost u ljetnim mjesecima (100% u razdoblju od srpnja do rujna). Zrele oocite u stadiju IV zabilježene su od studenog do travnja, sa najvišom zastupljenošću u siječnju (26.7%). Povećanje postotka oocita u stadijima II i III uočeno je u listopadu i ostaje gotovo nepromijenjeno do svibnja. Za ljetnjih mjeseci (svibanj–rujan), u testisima su bili prisutni samo spermatogoniji stadija I. U listopadu se započinju pojavljivati spermatociti (stadij II), a od studenog i spermatidi (stadij III). Spermatozoi (stadij IV) se javljaju u prosincu, a najvišu razinu dostižu u tijekom siječnja i veljače.

An additional record of one of the most successful lessepsian migrants in the Mediterranean, Fistularia commersonii (Osteichthyes: Fistularidae), indicates the spreading of this species in the Adriatic Sea. This is by far the only lessepsian migrant with multiple confirmed records, separated in time and space, for the Adriatic. Its fast spreading is followed by rapid population explosion at invaded areas and potential effects on the local fish fauna are emphasized.

The recruitment of leaping grey mullet, Liza saliens juveniles in the Neretva River estuary (middle eastern Adriatic Sea, Croatia) started in July, was highest in August, decreased in September, and was unobserved later. The juveniles firstly occupied mouths of rivers and channels and then started migrating to more freshwater habitats. By feeding investigation, the harpacticoid copepods were the dominant prey in the stomachs of L. saliens juveniles during the period from August to December and nematodes replaced those as the dominant prey from January to April. Plant material in the stomachs started to appear at 24 mm of standard length, and was common prey in fish larger than 26 mm. In comparison with other Adriatic mullet’s species, the leaping grey mullet juveniles first started to recruit during July and August, while other grey mullet species appeared later (in September) in the Neretva River estuary. Such intraspecies spawning and recruitment segregation together with the optimal quantity and quality of available and preferable prey could provide an explanation for the recent success and propagation of this species in this area., Novačenje mlađi cipla dugaša, Liza saliens na području estuarija rijeke Neretve počinje u srpnju, najveće je u kolovozu, smanjuje se u rujnu, nakon čega nije zabilježeno. Mlađ se najprije pojavljuje na ušćima rijeka i kanala, a zatim počinje migrirati prema vodama niže slanosti. U prehrani mlađi cipla dugaša od kolovoza do prosinca su dominirali harpatikoidni kopepodi, a od siječnja do travnja oblići. Biljni materijal se počinje pojavljivati u probavilima jedinki najmanje ukupne dužine 24 mm, a uobičajen je u probavilima riba većih od 26 mm. Uspoređujući s ostalim vrstama cipala u području estuarija rijeke Neretve koje se mrijeste u ljetno-jesenskom razdoblju, dugaš se počinje novačiti prvi, tijekom srpnja i kolovoza, dok se ostale vrste pojavljuju u rujnu. Ovo ranije mriještenje i novačenje mlađi bez nazočnosti drugih vrsta cipala, tijekom razdoblja optimalnog sastava i brojnosti hrane, može biti jedan od razloga recentne propagacije cipla dugaša na području estuarija Neretve.

Selectivity curves of pandora (Pagellus erythrinus, L. 1758) have been estimated in the areaof the Montenegrin shelf (South Adriatic), for trawls with bar mesh sizes of 13.5, 16.1, 17.5, 22and 35 mm (according to national legislation in Montenegro bar mesh size is measured from knotto knot which is, according to Commission Regulation (EC) No. 129/2003, stretched mesh sizedivided by 2). Although the existing national legislation prescribes that the minimum mesh size ofthe trawl be not smaller than 20 mm, trawls with forbidden mesh sizes are often used in this area.Results obtained showed that meshes smaller than 20 mm (13.5 mm, 16.1 mm, 17.5 mm) catch largenumbers of immature individuals, since the length of 50% retention (L50%) for all these meshes issmaller than the length of fish at first spawning and which is, for Pagellus erythrinus in Montenegrinwaters, ≈ 12 cm. L50% is longer than the average length of the first spawning only for nets withopenings of 22 mm and 35 mm bar mesh size., Krivulje selektivnosti arbuna (Pagellus erythrinus, L. 1758) procjenjivane su na kontinentalnoj podini crnogorskog primorja korištenjem dubinskih povlačnih mreža (koća) čija je veličina oka na saki mreže iznosila 13.5 mm, 16.1 mm, 17.5 mm, 22 mm i 35 mm, (mjereno od čvora do čvora). Rezultati istraživanja pokazuju kako su se mreže, čija minimalna veličina oka na saki ne smije biti manja od 20 mm, na ovom području upotrebljavale i sa manjim veličinama oka od propisane. Rezultati dobiveni istraživanjima pokazuju da su mreže čija je veličina oka bila ispod 20 mm (zakonom propisana minimalna veličina oka) lovile izrazito veliki broj spolno nezrelih jedinki, a 50% dužina zadržavanja u mreži (L50%) za navedene mreže bila je ispod dužine kod koje nastupa prva spolna zrelostost kod ove vrste (12 cm). Za mreže čija je veličina oka bila veća od 20 mm, dužina L50% bila je veća od dužine pri kojoj nastupa prva spolna zrelost. Provedena istraživanja potvrđuju opravdanost reguliranja veličine oka na pridnenim povlačnim mrežama kao jednog od ključnih čimbenika zaštite nedoraslih primjeraka, te samim time i osiguravanja dugoročno održivog iskorištavanja obnovljivih bogatstava mora.

Hematological Profile of Serbian Youth National Soccer Teams Soccer is one of the most widely played and complex sports in the world, where players need technical, tactical, and physical skills to succeed. Technical and tactical skills in soccer are highly dependent on the player's physical capacity. The selection, development and professional guidance of young players is a priority for many top soccer clubs in order to maintain their sporting and financial status. The aim of the present study was to determine hematological profile of youth national soccer teams and to compare the values of fifteen hematological parameters between 3 Serbian youth national teams (under 14, 15 and 16 years old), as well as between soccer players and nonathletes. 80 young soccer players and 30 non-athletes participated in the study. 15 hematologic parameters (WBC, RBC, HGB, HCT, PLT, MCV, MCH, MCHC, PDW, LYM%, MON%, GRAN%, LYM, MON, GRAN) were measured. In order to determine the significance of differences between the groups on a multivariate level a multivariate analysis of variance (MANOVA) was administered, and to test the differences between the groups on an univariate level a univariate analysis of variance (ANOVA) was applied. It was concluded that there is no significant difference in all the variables (WBC, Ly, Mo, Gr, PLT, HGB, HCT, etc), except RBC, probably due to age, androgen affection on erythropoesis, field positoning and diet. From a practical point of view, the clinician has to take into account not only age, but also training status of individuals when evaluating their blood tests.