33 results on '"Adorno, G."'
Search Results
2. THE SOCIAL CONSTRUCTION OF EMERGING ELDERS: IMPLICATIONS FOR AGE-FRIENDLY COMMUNITY ASSESSMENTS
- Author
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Fields, N., primary, Adorno, G., additional, Felderhoff, B., additional, Parekh, R., additional, and Magruder, K., additional
- Published
- 2017
- Full Text
- View/download PDF
3. AGING IN LOW-DENSITY URBAN ENVIRONMENTS: TRANSIT MOBILITY AS A SOCIAL JUSTICE ISSUE
- Author
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Adorno, G., primary, Fields, N., additional, Parekh, R., additional, and Magruder, K., additional
- Published
- 2017
- Full Text
- View/download PDF
4. A new system for quality control in hematopoietic progenitor cell units before reinfusion in autologous transplant
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Scerpa, M, Rossi, C, Daniele, N, Lanti, A, Adorno, G, Picardi, A, Arcese, W, Amadori, S, Isacchi, G, and Zinno, F
- Subjects
Cryopreservation ,Quality Control ,Transplantation ,Cell Proliferation ,Cell Survival ,Flow Cytometry ,Hematopoietic Stem Cell Transplantation ,Hematopoietic Stem Cells ,Humans ,Transplantation, Autologous ,Hematology ,Immunology ,Immunology and Allergy ,Settore MED/15 ,Settore MED/05 - Patologia Clinica ,Settore MED/15 - Malattie del Sangue ,Autologous - Abstract
In our Center, the cell viability, the integrity of the bag, and the clonogenic assay were evaluated before the reinfusion of hematopoietic progenitor cells-apheresis (HPC-A). This quality control (QC) should be made 14 days before the reinfusion to the patient to have the result of the functional test on the proliferative capacity of hematopoietic progenitors.This study was designed to assess the potential of an automatic cell counting system (NucleoCounter NC-3000, ChemoMetec) in our clinical routine as a support of the clonogenic assay and the cytofluorimetric analysis for the QC of the cryopreserved HPC-A. The cell viability was evaluated by flow cytometry using the modified International Society of Hematotherapy and Graft Engineering protocol. The proliferative potential was assessed by specific clonogenic tests using a commercial medium. Furthermore, we evaluated the cellular functionality with NucleoCounter NC-3000, by using two protocols: "vitality assay" and "mitochondrial potential assay."The evaluation of the total nucleated cells in preapoptosis measured by 5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimidazol-carbocyanine iodide (JC-1) assay showed a negative correlation (r=-0.43) with the total number of colonies (colony-forming unit [CFU]-granulocyte-macrophage progenitors plus burst-forming unit-erythroid progenitors plus CFU-granulocyte, erythroid, macrophage, megakaryocyte progenitors) obtained after seeding of 50 × 10(6) /L viable total nucleated cells. We observed a significant difference (p0.0001) comparing the median number of colonies (166.70; SD, ± 136.36) obtained with a value of JC-1 less than 30% to the number of colonies (61.75; SD, ± 59.76) obtained with a value of JC-1 more than 30%.The evaluation of cell functionality by the use of the NucleoCounter NC-3000 is in agreement with results from clonogenic assay and can be considered an effective alternative in the routine laboratory.
- Published
- 2014
5. Serum thymosin α 1 levels in patients with chronic inflammatory autoimmune diseases
- Author
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Pica, F, primary, Chimenti, M S, additional, Gaziano, R, additional, Buè, C, additional, Casalinuovo, I A, additional, Triggianese, P, additional, Conigliaro, P, additional, Di Carlo, D, additional, Cordero, V, additional, Adorno, G, additional, Volpi, A, additional, Perricone, R, additional, and Garaci, E, additional
- Published
- 2016
- Full Text
- View/download PDF
6. The processing of stem cell concentrates from the bone marrow in ABO-incompatible transplants: how and when
- Author
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Daniele, N, Scerpa, M, Rossi, C, Lanti, A, Adorno, G, Isacchi, G, and Zinno, F
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Stem Cells ,Preservation, Biological ,Settore MED/05 - Patologia Clinica ,Humans ,Bone Marrow Cells ,Cell Separation ,Review ,Biological ,Allografts ,Preservation ,ABO Blood-Group System ,Stem Cell Transplantation - Published
- 2013
7. Apheresis in the treatment of recalcitrant atopic dermatitis: Case series and review of the literature
- Author
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Chiricozzi, Andrea, Faleri, S., Lanti, A., Adorno, G., Lore, B., Chimenti, S., Saraceno, R., Chiricozzi A. (ORCID:0000-0002-6739-0387), Chiricozzi, Andrea, Faleri, S., Lanti, A., Adorno, G., Lore, B., Chimenti, S., Saraceno, R., and Chiricozzi A. (ORCID:0000-0002-6739-0387)
- Abstract
Background: Atopic dermatitis is a chronic disabling inflammatory skin disorder, typically characterized by intensely itching, oozing, crusted, eroded vesicles or papules developing on erythematous plaques. Conventional treatments, both topical and systemic, may produce unsuccessful and unsatisfactory results. Objectives: we aimed to assess the efficacy of apheretic treatments in patients with severe, recalcitrant AD, in particular, the pruritic component. Patients and methods: four patients affected by recalcitrant and debilitating atopic dermatitis, who had previously received conventional topical and systemic therapies with poor clinical improvement, were treated with extracorporeal photopheresis or therapeutic plasma exchange. Results: a satisfactory response to apheresis was observed with a reduction of pruritus and skin lesions. Conclusion: In our experience, apheretic therapies might be used as monotherapy but, more effectively, in combination with topical and/or systemic treatments. Indeed, they proved to be a safe “enhancer” for increasing the efficacy of conventional therapeutics.
- Published
- 2014
8. Fuzzy Logic multiobjective optimisation for stand-alone photovoltaic plants
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Tina, G, Adorno, G, and Ragusa, CARLO STEFANO
- Published
- 1998
9. Fuzzy logic multi-objective optimisation for stand alone photovoltaic plants
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Adorno, G, Ragusa, C, and Tina, Giuseppe Marco
- Published
- 1998
10. FIRST REPORT OF T(8-21)(Q22-Q22) IN A CASE OF DE-NOVO ACUTE MONOBLASTIC LEUKEMIA
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Coxfroncillo, M, Genuardi, Maurizio, Bajer, J, Livdi, E, Adorno, G, Venditti, A, Masi, Maria Claudia, Giudiceandrea, P, Neri, G, Papa, G, GENUARDI, M (ORCID:0000-0002-7410-8351), MASI, M, Coxfroncillo, M, Genuardi, Maurizio, Bajer, J, Livdi, E, Adorno, G, Venditti, A, Masi, Maria Claudia, Giudiceandrea, P, Neri, G, Papa, G, GENUARDI, M (ORCID:0000-0002-7410-8351), and MASI, M
- Abstract
Here we describe the case of a 30-year-old man with a diagnosis of de novo acute monoblastic leukemia (FAB M5a), whose karyotype analysis revealed the presence of the translocation (8;21)(q22;q22) as the sole chromosome anomaly. In spite of the rather good prognosis patients suffering from acute leukemia and carrying this translocation are supposed to have, our patient had a very poor outcome, including an early relapse resistant to any treatment and meningeal localization. Death occurred within 5 months from diagnosis. To our knowledge this is the first report of t(8;21)(q22;q22) in de novo acute monoblastic leukemia.
- Published
- 1995
11. Multiparametric analysis for the enumeration of CD34+ cells from bone marrow and stimulated peripheral blood.
- Author
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Venditti, A, primary, Buccisano, F, additional, Del Poeta, G, additional, Battaglia, A, additional, Tamburini, A, additional, Bruno, A, additional, Adorno, G, additional, Caravita, T, additional, Santinelli, S, additional, Del Moro, B, additional, Epiceno, A M, additional, Ballatore, G, additional, and Amadori, S, additional
- Published
- 1998
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12. KI and WU polyomaviruses and CD4+ cell counts in HIV-1-infected patients, Italy.
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Babakir-Mina M, Ciccozzi M, Farchi F, Bergallo M, Cavallo R, Adorno G, Perno CF, Ciotti M, Babakir-Mina, Muhammed, Ciccozzi, Massimo, Farchi, Francesca, Bergallo, Massimiliano, Cavallo, Rossana, Adorno, Gaspare, Perno, Carlo Federico, and Ciotti, Marco
- Abstract
To investigate an association between KI and WU polyomavirus (KIPyV and WUPyV) infections and CD4+ cell counts, we tested HIV-1-positive patients and blood donors. No association was found between cell counts and virus infections in HIV-1-positive patients. Frequency of KIPyV infection was similar for both groups. WUPyV was more frequent in HIV-1-positive patients. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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13. Efficacy and safety of human leucocyte interferon-alpha treatment in patients younger than 60 years of age with polycythaemia vera.
- Author
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Stasi, R, Brunetti, M, Bussa, S, Venditti, A, Del Poeta, G, Conforti, M, Scimò, M T, Cudillo, L, Adorno, G, Cecconi, M, Amadori, S, and Pagano, A
- Published
- 1997
14. La trasfusione di sangue, gruppi sanguigni e principali disordini della coagulazione di interesse chirurgico
- Author
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Isacchi, G., Adorno, G, Cafolla, Arturo, Ciuffreda, M, Francesconi, M, and Malagnino, F.
- Published
- 1983
15. Kidney carcinoma revealed by autoimmune hemolytic anemia
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Gabriella GIRELLI, Adorno, G., Perrone, M. P., Arista, M. C., Cardillo, A., Vegna, M. L., Petti, M. C., and Mandelli, F.
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Adult ,Male ,Humans ,Anemia, Hemolytic, Autoimmune ,Adenocarcinoma ,Kidney Neoplasms
16. L'Immacolatella nel Porto di Napoli: dall’abbandono alla riaffermazione di un’identità 'migrante'
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Stefania Pollone, S. Adorno, G. Cristina, A. Rotondo, and Pollone, Stefania
- Subjects
Domenico Antonio Vaccaro, architettura del XVIII secolo, Porto di Napoli, emigrazione, conservazione - Abstract
Ultimo brano di quel complesso insieme di trasformazioni urbane che, intrapreso a partire dal quarto decennio del XVIII secolo per volere del re Carlo III di Borbone, modificò profondamente il rapporto della città di Napoli con il suo mare, l’edificio dell’Immacolatella conserva la memoria di una delle pagine più dolorose della storia del Meridione d’Italia. Quale sede della Capitaneria e dell’Ufficio di Sanità Marittima, infatti, il manufatto è, tra la fine dell’Ottocento e i primi decenni del Novecento, luogo di passaggio per le migliaia di emigranti che, giunti in città, si sottoponevano ai controlli sanitari prima di imbarcarsi verso nuove patrie. Piccola Ellis Island partenopea, l’Immacolatella manifesta il suo ruolo di testimonianza vivente del passaggio di generazioni di uomini che hanno varcato le sue soglie sperando in un futuro migliore: essa incarna i valori negativi dell’indigente povertà e del dolore del distacco e, al contempo, quelli positivi riposti nei sogni e nelle speranze. Nonostante l’indiscussa qualità formale e materica dell'architettura settecentesca e la carica emotiva che essa veicola, l’edificio appare caduto nell’oblio più profondo, reclamando urgenti opere di salvaguardia. Vittima di trasformazioni e di usi inappropriati avvicendatisi nel corso degli ultimi decenni, la fabbrica è soggetta agli attacchi marini e meteorici nonché alle conseguenze dell’incuria e dell’abbandono. Tenuto conto di tali problematiche - tangibili e intangibili - il contributo si pone l'obiettivo di esplorare le complesse valenze della fabbrica attraverso fonti iconografiche e inedite fonti d'archivio nonché mettere in risalto quelle tracce, spesso poco discernibili, che la storia ha lasciato sul costruito per riflettere, infine, sulle possibilità di contemperamento tra esigenze restaurative e necessità di riaffermazione di un frammento di un'identità dimenticata nel porto di Napoli.
- Published
- 2014
17. Ferrara nelle cronache rinascimentali: spazi urbani e paradigmi del potere estense
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Saletti, B, non c'è un autore, solo curatori, S. Adorno, G. Cristina, A. Rotondo, and Saletti, Beatrice
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Urban History, Medieval History, Cultural History, Este family ,Spazi urbani e letteratura ,Socio-culturale ,Ferrara nel Rinascimento ,Urban History ,Cultural History ,Este family ,Medieval History ,Cronache medievali - Abstract
Nell’affrontare lo studio comparato di alcune dozzine di cronache ferraresi quattro-cinquecentesche si staglia con evidenza la percezione, da parte di più autori, di un vissuto urbano dai precisi significati sociali: aree dedicate alla mercatura, altre “residenziali”, altre assolutamente artificiali (in occasione, ad esempio, delle entrate trionfali). Se tale processo è comune a un elevato numero di cittadine italiane del periodo, è tuttavia innegabile il ruolo degli Este nel modificare radicalmente il volto della città capitale del loro marchesato e ducato, tramite ardite e massicce imprese architettoniche che le cronache, di qualsivoglia autore, non possono tacere. È sulla città in evoluzione, sui significati percepiti dei suoi cambiamenti, che si articola l’intervento.
- Published
- 2014
18. La Pianta dell’Isola di Ponza di Agostino Grasso. Indagini e prodotti cartografici come strumento di conoscenza del territorio e di lettura delle dinamiche insediative (XVIII secolo)
- Author
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GALLIA, ARTURO, AA.VV, S. Adorno, G. Cristina, A. Rotondo, and Gallia, Arturo
- Published
- 2014
19. Characterization and Clinical Assessment of a Peculiar Case of Hemolytic Anemia.
- Author
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Castelgrande F, Viola G, Calabrese C, Iozzo M, Massoud R, Pieri M, Minieri M, Adorno G, Bernardini S, and Terrinoni A
- Abstract
Thalassemic diseases are characterized by a reduced (β
+ ) or absent (β0 ) synthesis of the globin chains of hemoglobin (Hb) due to genetic mutations. β-thalassemia was more frequent in the Mediterranean area, but now it is diffused worldwide. Three possible genetic forms can be distinguished: β0 /β0 , the most severe (Cooley's disease); β0 /β+ of intermediate severity; β+ /β+ associated with β-thalassemia intermedia or minor. Recently, a clinical non-genetic classification has been proposed: transfusion-dependent thalassemia (TDT), requiring regular lifetime blood transfusions, and non-transfusion-dependent thalassemia (NTDT), requiring occasional transfusions to manage acute cases. In this report, we studied a patient whose blood count indicated a severe anemia but also showed thrombocytosis, leukocytosis, and an elevated number of nucleated red blood cells (NRBC). These altered blood parameters suggested initially a possible diagnosis of hemoglobinopathy or myeloproliferative syndrome. The molecular and genetic analyses demonstrated the presence of HbF (5.3%) and HbA2 (7.7%) and the presence of the homozygote mutation (IVS1.6T>C) in the β-globin gene. According to these data, a diagnosis of β-thalassemia intermedia form has been proposed. Nevertheless, the clinical condition, the presence of thrombocytosis, leukocytosis, an elevated number of NRBC, and the frequent blood transfusions lead to reclassification of the patient as TDT subject. Consequently, this result suggests that a unique genotype-phenotype correlation is not possible in the presence of β+ mutations since other concomitant pathologies can exacerbate the disease., Competing Interests: The authors declare that they have no conflict of interest., (Copyright 2024, Castelgrande et al.)- Published
- 2024
- Full Text
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20. Urine Parameters in Patients with COVID-19 Infection.
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Morello M, Amoroso D, Losacco F, Viscovo M, Pieri M, Bernardini S, and Adorno G
- Abstract
A urine test permits the measure of several urinary markers. This is a non-invasive method for early monitoring of potential kidney damage. In COVID-19 patients, alterations of urinary markers were observed. This review aims to evaluate the utility of urinalysis in predicting the severity of COVID-19. A total of 68 articles obtained from PubMed studies reported that (i) the severity of disease was related to haematuria and proteinuria and that (ii) typical alterations of the urinary sediment were noticed in COVID-19-associated AKI patients. This review emphasizes that urinalysis and microscopic examination support clinicians in diagnosing and predicting COVID-19 severity.
- Published
- 2023
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21. Platelet-Derived miR-126-3p Directly Targets AKT2 and Exerts Anti-Tumor Effects in Breast Cancer Cells: Further Insights in Platelet-Cancer Interplay.
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Sibilano M, Tullio V, Adorno G, Savini I, Gasperi V, and Catani MV
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- Blood Platelets metabolism, Cell Line, Tumor, Female, Humans, Breast Neoplasms blood, Breast Neoplasms metabolism, MicroRNAs metabolism, Proto-Oncogene Proteins c-akt metabolism
- Abstract
Among the surrounding cells influencing tumor biology, platelets are recognized as novel players as they release microvesicles (MVs) that, once delivered to cancer cells, modulate signaling pathways related to cell growth and dissemination. We have previously shown that physiological delivery of platelet MVs enriched in miR-126 exerted anti-tumor effects in different breast cancer (BC) cell lines. Here, we seek further insight by identifying AKT2 kinase as a novel miR-126-3p direct target, as assessed by bioinformatic analysis and validated by luciferase assay. Both ectopic expression and platelet MV-mediated delivery of miR-126-3p downregulated AKT2 expression, thus suppressing proliferating and invading properties, in either triple negative (BT549 cells) or less aggressive Luminal A (MCF-7 cells) BC subtypes. Accordingly, as shown by bioinformatic analysis, both high miR-126 and low AKT2 levels were associated with favorable long-term prognosis in BC patients. Our results, together with the literature data, indicate that miR-126-3p exerts suppressor activity by specifically targeting components of the PIK3/AKT signaling cascade. Therefore, management of platelet-derived MV production and selective delivery of miR-126-3p to tumor cells may represent a useful tool in multimodal therapeutic approaches in BC patients.
- Published
- 2022
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22. Matched-Pair Analysis of Transplant from Haploidentical, Unmanipulated Bone Marrow Donor versus HLA Identical Sibling for Patients with Hematologic Malignancies.
- Author
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Arcese W, Cerretti R, Sarmati L, Cudillo L, De Angelis G, Mariotti B, Bruno A, Mangione I, Rapanotti C, Andreani M, De Fabritiis P, Dentamaro T, Cupelli L, Mengarelli A, Marchesi F, Tirindelli MC, Annibali O, Tafuri A, Ferrari A, Cedrone M, Anaclerico B, Adorno G, Miccichè S, Andreoni M, and Picardi A
- Subjects
- Bone Marrow, Humans, Matched-Pair Analysis, Neoplasm Recurrence, Local, Siblings, Graft vs Host Disease prevention & control, Hematologic Neoplasms therapy
- Abstract
A matched-pair analysis of transplant-related outcomes was carried out in 116 of 255 consecutive patients who received transplants from an HLA identical sibling (n = 58) or haploidentical related donor (n = 58). The 2 patient series were matched with 9 variables: period of transplant, patient and donor age, sex, diagnosis, disease phase, conditioning regimen, donor-recipient sex, and cytomegalovirus (CMV) status combinations. As graft-versus-host disease (GVHD) prophylaxis, all patients received the standard cyclosporine and methotrexate association with the addition of anti-thymocyte globulins, mycophenolate mofetil, and basiliximab in haploidentical, unmanipulated bone marrow recipients. Anti-infectious management, transfusion policy, and supportive care were identical for all patients. By comparing the 2 patient series, no statistically significant difference was observed for the cumulative incidence of advanced acute and extensive chronic GVHD, transplant-related mortality, and relapse. With a median follow-up of 3.5 years, the 5-year disease-free survival was 37% ± 6% and 36% ± 6% for HLA identical sibling and haploidentical recipients, respectively. The results of transplant from HLA identical siblings and haploidentical donors are comparable. Regardless of the HLA matching, other factors known to affect the transplant outcomes, such as donor-recipient age, sex, and CMV status combinations, might drive the search for the best donor., (Copyright © 2020 American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. All rights reserved.)
- Published
- 2020
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23. The Social Construction of "Emerging Elders": Implications for Age-Friendly Community Assessments.
- Author
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Fields NL, Adorno G, Felderhoff B, Parekh Msw R, Miller Msw V, Magruder K, and Rogers K
- Abstract
The term "emerging elders" has surfaced in age-friendly community assessment tools to denote a subset of older adults; however, limited guidance is provided on its application to aging populations. The goal of this study was to develop a data-driven conceptualization of "emerging elders" as part of an age-friendly community assessment. Adults, aged 55 years and above, were asked about their subjective meaning of "emerging elder" within the context of a larger study of aging well in a large U.S. metropolitan city. Using inductive and deductive methods, the researchers analyzed qualitative data ( N = 38) collected from individual interviews with homebound older adults ( n = 15) and participants of three focus groups ( n = 23). Four themes suggest that emerging elderhood is related to chronological age, functional ability, transitions, and self-identity. Findings suggest that the term emerging elderhood may foster negative images of older adults consistent with Western cultural discourse, despite the positive connotations associated with "emerging elder" in indigenous and spiritual communities. Findings underscore the need to further refine age-friendly community assessments that take into account the social constructions ascribed to older adults and need for strategies to engage emerging elders in future research of age-friendly communities., Competing Interests: Declaration of Conflicting Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2018
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24. In Vitro Exposure to Prostratin but Not Bryostatin-1 Improves Natural Killer Cell Functions Including Killing of CD4 + T Cells Harboring Reactivated Human Immunodeficiency Virus.
- Author
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Desimio MG, Giuliani E, Ferraro AS, Adorno G, and Doria M
- Abstract
In the attempt of purging the HIV-1 reservoir through the "shock-and-kill" strategy, it is important to select latency-reversing agents (LRAs) devoid of deleterious effects on the antiviral function of immune effector cells. Here, we investigated two LRAs with PKC agonist activity, prostratin (PRO) and bryostatin-1 (BRY), for their impact on the function of natural killer (NK) cells, the major effectors of innate immunity whose potential in HIV-1 eradication has emerged in recent clinical trials. Using NK cells of healthy donors, we found that exposure to either PRO or BRY potently activated NK cells, resulting in upmodulation of NKG2D and NKp44 activating receptors and matrix metalloprotease-mediated shedding of CD16 receptor. Despite PRO and BRY affected NK cell phenotype in the same manner, their impact on NK cell function was diverse and showed considerable donor-to-donor variation. Altogether, in most tested donors, the natural cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC) of NK cells were either improved or maintained by PRO, while both activities were impaired by BRY. Moreover, we analyzed the effect of these drugs on the capacity of treated NK cells to kill autologous latently infected CD4
+ T cells reactivated via the same treatment. First, we found that PRO but not BRY increased upmodulation of the ULBP2 ligand for NKG2D on reactivated p24+ cells. Importantly, we showed that clearance of reactivated p24+ cells by NK cells was enhanced when both targets and effectors were exposed to PRO but not to BRY. Overall, PRO had a superior potential compared with BRY as to the impact on key NK cell functions and on NK-cell-mediated clearance of the HIV-1 reservoir. Our results emphasize the importance of considering the effects on NK cells of candidate "shock-and-kill" interventions. With respect to combinative approaches, the impact on NK cells of each LRA should be re-evaluated upon combination with a second LRA, which may have analogous or opposite effects, or with immunotherapy targeting NK cells. In addition, avoiding co-administration of LRAs that negatively impact ADCC activity by NK cells might be essential for successful application of antibodies or vaccination to "shock-and-kill" strategies.- Published
- 2018
- Full Text
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25. Haploidentical HSCT for hemoglobinopathies: improved outcomes with TCRαβ + /CD19 + -depleted grafts.
- Author
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Gaziev J, Isgrò A, Sodani P, Paciaroni K, De Angelis G, Marziali M, Ribersani M, Alfieri C, Lanti A, Galluccio T, Adorno G, and Andreani M
- Subjects
- Adolescent, Antigens, CD19, Antigens, CD34, Child, Child, Preschool, Graft Rejection, Graft vs Host Disease etiology, Hematopoietic Stem Cell Transplantation adverse effects, Hemoglobinopathies complications, Hemoglobinopathies mortality, Humans, Male, Receptors, Antigen, T-Cell, alpha-beta, Retrospective Studies, Survival Analysis, Transplantation, Haploidentical adverse effects, Treatment Outcome, Graft Survival immunology, Hematopoietic Stem Cell Transplantation methods, Hemoglobinopathies therapy, Lymphocyte Depletion methods, Transplantation, Haploidentical methods
- Abstract
We examined outcomes of haploidentical hematopoietic cell transplantation (haplo-HCT) using T-cell receptor αβ
+ (TCRαβ+ )/CD19+ -depleted grafts (TCR group, 14 patients) in children with hemoglobinopathies. Patients received a preparative regimen consisting of busulfan, thiotepa, cyclophosphamide, and antithymocyte globulin preceded by fludarabine, hydroxyurea, and azathioprine. The median follow-up among surviving patients was 3.9 years. The 5-year probabilities of overall survival (OS) and disease-free survival (DFS) were 84% and 69%, respectively. The incidence of graft failure was 14%. We compared outcomes to a historical group of 40 patients with hemoglobinopathies who received CD34+ -selected grafts (CD34 group). The median follow-up of surviving patients for the CD34 group was 7.5 years. The 5-year probabilities of OS and DFS were 78% and 39%, respectively. The CD34 group had a significantly higher incidence of graft failure (45%) than the TCR group (14%) ( P = .048). The incidences of grades 2 to 4 acute graft-versus-host disease (GVHD) in the TCR and CD34 groups were 28% and 29%, respectively, and 21% and 10% ( P = .1), respectively, for extensive chronic GVHD. Viral reactivation was common in both groups. The overall incidence of posttransplant lymphoproliferative disorders for the entire group was 16%. Among all patients, 5 developed autoimmune hemolytic anemia or thrombocytopenia, with the overall cumulative incidence of 11%. The 2 groups showed suboptimal CD4+ recovery within the first 6 months of transplantation with no significant difference between groups. These data demonstrate that TCRαβ+ /CD19+ -depleted grafts are associated with a reduced incidence of graft failure, but delayed immune reconstitution and associated morbidity and mortality remain a significant challenge., (© 2018 by The American Society of Hematology.)- Published
- 2018
- Full Text
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26. The processing of stem cell concentrates from the bone marrow in ABO-incompatible transplants: how and when.
- Author
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Daniele N, Scerpa MC, Rossi C, Lanti A, Adorno G, Isacchi G, and Zinno F
- Subjects
- Allografts, Cell Separation instrumentation, Humans, Preservation, Biological instrumentation, ABO Blood-Group System, Bone Marrow Cells cytology, Cell Separation methods, Preservation, Biological methods, Stem Cell Transplantation, Stem Cells cytology
- Published
- 2014
- Full Text
- View/download PDF
27. Haploidentical, unmanipulated, G-CSF-primed bone marrow transplantation for patients with high-risk hematologic malignancies.
- Author
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Di Bartolomeo P, Santarone S, De Angelis G, Picardi A, Cudillo L, Cerretti R, Adorno G, Angelini S, Andreani M, De Felice L, Rapanotti MC, Sarmati L, Bavaro P, Papalinetti G, Di Nicola M, Papola F, Montanari M, Nagler A, and Arcese W
- Subjects
- Adolescent, Adult, Aged, Child, Child, Preschool, Disease-Free Survival, Female, Follow-Up Studies, Graft Survival, Graft vs Host Disease blood, Graft vs Host Disease mortality, Graft vs Host Disease therapy, Hematologic Neoplasms blood, Humans, Male, Middle Aged, Neutrophils metabolism, Risk Factors, Survival Rate, Time Factors, Transplantation, Homologous, Bone Marrow Transplantation, Granulocyte Colony-Stimulating Factor administration & dosage, Hematologic Neoplasms mortality, Hematologic Neoplasms therapy, Living Donors
- Abstract
Unlabelled: Eighty patients with high-risk hematologic malignancies underwent unmanipulated, G-CSF–primed BM transplantation from an haploidentical family donor. Patients were transplanted in first or second complete remission (CR, standard-risk: n =45) or in > second CR or active disease (high-risk: n =35). The same regimen for GVHD prophylaxis was used in all cases. The cumulative incidence (CI) of neutrophil engraftment was 93% 0.1%. The 100-day CIs for II-IV and III-IV grade of acute GVHD were 24% 0.2% and 5% 0.6%, respectively. The 2-year CI of extensive chronic GVHD was 6% 0.1%. The 1-year CI of treatment-related mortality was 36% 0.3%. After a median follow-up of 18 months, 36 of 80 (45%) patients are alive in CR. The 3-year probability of overall and disease-free survival for standard-risk and high-risk patients was 54% 8% and 33% 9% and 44% 8% and 30% 9%, respectively. In multivariate analysis, disease-free survival was significantly better for patients who had standard-risk disease and received transplantations after 2007. We conclude that unmanipulated, G-CSF–primed BM transplantation from haploidentical family donor provides very encouraging results in terms of engraftment rate, incidence of GVHD and survival and represents a feasible, valid alternative for patients with high-risk malignant hematologic diseases, lacking an HLA identical sibling and in need to be urgently transplanted., Key Points: Haploidentical, unmanipulated, G-CSF-primed bone marrow transplantation. Haploidentical hematopoietic stem cell transplantation for hematologic malignancies.
- Published
- 2013
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28. "It's Like Being a Well-Loved Child": Reflections From a Collaborative Care Team.
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Sanchez K and Adorno G
- Abstract
Objective: The present case study examines how a collaborative care model for the treatment of depression works with a low-income, uninsured adult population in a primary care setting., Method: The qualitative interviews were conducted in 2010 at a primary care clinic as part of an evaluation of the Integrated Behavioral Health program, a collaborative care model of identifying and treating mild-to-moderate mental disorders in adults in a primary care setting. A single-case study design of an interdisciplinary team was used: the care manager, the primary care physician, the consulting psychiatrist, and the director of social services. Other units of analysis included clinical outcomes and reports that describe the patient demographics, services offered, staff, and other operational descriptions., Results: Multiple themes were identified that shed light on how one primary care practice successfully operationalized a collaborative care model, including the tools they used in novel ways, the role of team members, and perceived barriers to sustainability., Conclusions: The insights captured by this case study allow physicians, mental health practitioners, and administrators a view into key elements of the model as they consider implementation of a collaborative care model in their own settings. It is important to understand how the model operates on a day-to-day basis, with careful consideration of the more subtle aspects of the program such as team functioning and adapting tools to new processes of care to meet the needs of patients in unique contexts. Attention to barriers that still exist, especially regarding workforce and workload, will continue to be critical to organizations attempting integration.
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- 2013
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29. T cell-depleted hla-haploidentical stem cell transplantation in thalassemia young patients.
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Sodani P, Isgrò A, Gaziev J, Paciaroni K, Marziali M, Simone MD, Roveda A, De Angelis G, Gallucci C, Torelli F, Isacchi G, Zinno F, Landi F, Adorno G, Lanti A, Testi M, Andreani M, and Lucarelli G
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- 2011
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30. Immunohematologic reconstitution in pediatric patients after T cell-depleted HLA-haploidentical stem cell transplantation for thalassemia.
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Isgrò A, Marziali M, Sodani P, Gaziev J, Erer B, Polchi P, Paciaroni K, Roveda A, De Angelis G, Gallucci C, Alfieri C, Simone MD, Zinno F, Isacchi G, Adorno G, Lanti A, Leti W, Aiuti F, Fraboni D, Andreani M, and Lucarelli G
- Subjects
- B-Lymphocytes cytology, Blood Cells cytology, Bone Marrow Cells cytology, Bone Marrow Cells metabolism, CD4-Positive T-Lymphocytes cytology, CD8-Positive T-Lymphocytes cytology, Cell Count, Child, Child, Preschool, Chimera blood, Colony-Forming Units Assay, Graft Rejection immunology, Graft Survival immunology, HLA Antigens genetics, HLA Antigens immunology, Humans, Interleukin-2 metabolism, Interleukin-7 metabolism, Killer Cells, Natural cytology, Living Donors, Lymphocyte Count, Mothers, Stromal Cells cytology, Stromal Cells metabolism, T-Lymphocyte Subsets cytology, Transplants, Treatment Outcome, Tumor Necrosis Factor-alpha metabolism, Hematopoietic Stem Cell Transplantation methods, Histocompatibility, Maternal-Fetal, Lymphocyte Depletion, Lymphocytes cytology, T-Lymphocytes cytology, beta-Thalassemia therapy
- Abstract
To analyze immunohematologic reconstitution, particularly of natural killer (NK) cells, we evaluated 13 β-thalassemia patients after 20 and 60 days and 1 year posttransplantation with T cell-depleted HLA-haploidentical stem cells. We assessed lymphocyte and bone marrow (BM) progenitor cell phenotype and differentiation capacity, spontaneous BM cytokine production, stromal cells, and stromal cell interleukin (IL)-7 production. A reduced clonogenic capability manifested at day +20. Patients had significantly lower CD4(+) T cells versus controls, mainly in the CD45RA(+)CD62L(+) subset. NKs were among the first lymphocytes to repopulate the peripheral blood. At day +60, an increase in primitive BM progenitor cells paralleled small increases in CD4(+), naïve CD4(+), and thymic naïve Th cells. A significant increase in CD4(+) and CD8(+) markers paralleled an increase in CD3⁻CD16(+) NKs, especially with full engraftment. In patients with stable mixed chimerism we observed very low levels of CD3(+) donor chimerism early after transplant that increased over time, but a stable population of high donor NK cells, suggesting a role of these cells on donor engraftment. Stromal cells secreted less IL-7 and displayed "macrophage-like" morphology. Patients initially manifested impaired stem/progenitor cell growth and differentiation capacity in parallel with altered T cell homeostasis and a reduced T cell naïve compartment. We hypothesize that T cell compartment damage partly arises from altered new T cell production from the hematopoietic stem/progenitor cells under stromal cytokine influence. NNK subset analysis might be useful for determining transplant outcome., (Copyright © 2010 American Society for Blood and Marrow Transplantation. Published by Elsevier Inc. All rights reserved.)
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- 2010
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31. Purified T-depleted, CD34+ peripheral blood and bone marrow cell transplantation from haploidentical mother to child with thalassemia.
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Sodani P, Isgrò A, Gaziev J, Polchi P, Paciaroni K, Marziali M, Simone MD, Roveda A, Montuoro A, Alfieri C, De Angelis G, Gallucci C, Erer B, Isacchi G, Zinno F, Adorno G, Lanti A, Faulkner L, Testi M, Andreani M, and Lucarelli G
- Subjects
- Adolescent, Adult, Child, Child, Preschool, Feasibility Studies, Flow Cytometry, Graft Survival immunology, HLA Antigens metabolism, Humans, In Situ Hybridization, Fluorescence, Middle Aged, Mothers, Pilot Projects, Polymerase Chain Reaction, Prospective Studies, Transplantation Conditioning, Transplantation, Homologous, Young Adult, Antigens, CD34 metabolism, Bone Marrow Transplantation, Graft vs Host Disease prevention & control, Lymphocyte Depletion, Peripheral Blood Stem Cell Transplantation, T-Lymphocytes, Thalassemia therapy
- Abstract
Fetomaternal microchimerism suggests immunological tolerance between mother and fetus. Thus, we performed primary hematopoietic stem cell transplantation from a mismatched mother to thalassemic patient without an human leukocyte antigen-identical donor. Twenty-two patients with thalassemia major were conditioned with 60 mg/kg hydroxyurea and 3 mg/kg azathioprine from day -59 to -11; 30 mg/m(2) fludarabine from day -17 to -11; 14 mg/kg busulfan starting on day -10; and 200 mg/kg cyclophosphamide, 10 mg/kg thiotepa, and 12.5 mg/kg antithymocyte globulin daily from day -5 to -2. Fourteen patients received CD34(+)-mobilized peripheral blood and bone marrow progenitor cells; 8 patients received marrow graft-selected peripheral blood stem cells CD34(+) and bone marrow CD3/CD19-depleted cells. T-cell dose was adjusted to 2 x 10(5)/kg by fresh marrow cell addback at the time of transplantation. Both groups received cyclosporine for graft-versus-host disease prophylaxis for 2 months after transplantation. Two patients died (cerebral Epstein-Barr virus lymphoma or cytomegalovirus pneumonia), 6 patients reject their grafts, and 14 showed full chimerism with functioning grafts at a median follow-up of 40 months. None of the 14 patients who showed full chimerism developed acute or chronic graft-versus-host disease. These results suggest that maternal haploidentical hematopoietic stem cell transplantation is feasible in patients with thalassemia who lack a matched related donor.
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- 2010
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32. Positive immunomagnetic CD34(+) cell selection in haplo-identical transplants in beta-thalassemia patients: removal of platelets using an automated system.
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Zinno F, Landi F, Aureli V, Balduino G, Lanti A, Sodani P, Adorno G, Lucarelli G, and Isacchi G
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- Adolescent, Adult, Automation methods, B-Lymphocytes cytology, B-Lymphocytes immunology, Blood Platelets cytology, Cell Count, Child, Child, Preschool, Equipment Contamination prevention & control, Female, Graft Rejection immunology, Graft Rejection physiopathology, Graft Rejection prevention & control, Graft vs Host Disease immunology, Graft vs Host Disease physiopathology, Graft vs Host Disease prevention & control, Humans, Leukapheresis, Male, Middle Aged, T-Lymphocytes cytology, T-Lymphocytes immunology, Transplantation Conditioning, Young Adult, beta-Thalassemia immunology, beta-Thalassemia physiopathology, Antigens, CD34 immunology, Blood Platelets physiology, Immunomagnetic Separation methods, Stem Cell Transplantation methods, beta-Thalassemia surgery
- Abstract
Background Aims: Immunomagnetic CD34(+) cell selection (ICS) is utilized in autologous and allogeneic transplants. In the first case it is used to reduce the neoplastic contamination of concentrates, while in the second case it is needed to carry out a T-depletion of cell concentrates in order to reduce the incidence of graft-versus-host disease (GvHD) in patients who have undergone haplo-identical transplants., Methods: The efficacy of CliniMACS technology, after reduction of platelet contamination, incubation of monoclonal antibodies (MAb) and successive washings of concentrates, performed in 16 ICS using the standard method without reducing platelet content, was compared with the use of the automated system CytoMate, which was carried out in 46 ICS., Results: In the group of ICS carried out after automatic manipulation, a significant statistical difference in purity was noted (91.39% versus 83.57, P = 0.017) compared with the group of ICS carried out with the standard procedure. The same significant difference was noted in relation to the remaining percentages of CD3(+) and CD19(+) cells (2.31% versus 5.68%, P = 0.012, and 1.58% versus 2.71%, P = 0.014, respectively). Recovery of CD34+ cells overlapped in the two groups (70.49% versus 68.39%, P = 0.774)., Conclusions: Immunomagnetic selection carried out using the automated procedure was more efficient, producing a purer sample, more efficient T-depletion and optimal reduction of B cells, without influencing cell recovery. Furthermore, conforming to good manufacturing practice (GMP) guidelines, the entire procedure with CytoMate took place in a contamination-controlled environment.
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- 2010
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33. Diagnosis of acute myeloid leukemia and system Coulter VCS.
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Bruno A, Del Poeta G, Venditti A, Stasi R, Adorno G, Aronica G, Suppo G, Di Rienzo AM, Iazzoni R, and Tribalto M
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- Acute Disease, Humans, Predictive Value of Tests, Flow Cytometry, Leukemia, Myeloid diagnosis
- Abstract
Background: The aim of this study was to evaluate the possible contribution VCS could make in a hematological laboratory for the diagnosis of acute myeloid leukemia (AML)., Materials and Methods: Peripheral blood samples from 42 AML patients and 58 normal donors were analyzed by flow cytometry with the VCS. Normal and leukemic peripheral blood samples were tested to establish a correlation between VCS data and the reference manual method. We evaluated the sensitivity threshold of the VCS for blast cell detection in progressively diluted samples. We looked at a correlation between different scatterplots and flags and the FAB classification of acute myeloid leukemia in order to identify a characteristic VCS image for each subtype. Thirty-four bone marrow samples (18 normal donors and 16 leukemic patients) were analyzed by the VCS system to demonstrate a characteristic scattergram distribution. Further, we tried to compare scatterplots to the flags of leukemic bone marrow samples and, finally, we compared VCS scatterplots with aberrant antigen expression in AML cases., Results and Conclusions: Overall VCS specificity was 93.1% (54/58) in peripheral blood samples; sensitivity was 100% (42/42) and VCS efficiency was 96%. In AML the characteristic X6 flag was observed in 95.23% of the cases (40/42). In peripheral samples discrimination was made between AML M1 with agranular blasts > 50% of the non erythroid cells (NEC), M4, M5 on the one hand, and AML M1 with granular blasts > 50% of NEC, M2, M3 on the other: the X5 flag was often present in the second group because of the different localization of the cells (p = 0.001). In all normal bone marrow samples we observed granuloblasts in different maturation stages in the neutrophil region of the DF1 VCS scatterplot corresponding to the X5X6 flags or, rarely, to the X5X6X1, because of the presence of immature erythroid cells. This association X5X6 was never observed alone in patients affected by AML. In our study, it was difficult to identify peculiar scatterplots and alarms for each FAB class of AML. Nevertheless, we observed that in all M4 and M5 FAB cases the blastic cells both in the peripheral blood and in the bone marrow samples were located in the monocyte region, with the frequent presence of the X3 flag often associated with the X6 flag. Eight out of the 16 AML bone marrow samples (1 FAB M0, 1 M2, 1 M3, 2 M4, 3 M5) showed the X2 flag and partial localization of blasts in the lymphoid region. In all these cases the presence of some small blastic cells with agranular cytoplasm was confirmed by morphological observation and cytochemical stainings.
- Published
- 1994
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