Search

Your search keyword '"Adèle Arlat"' showing total 4 results
Start Over Author "Adèle Arlat" Search Limiters Available in Library Collection
4 results on '"Adèle Arlat"'

1. Generation of functionally active resident macrophages from adipose tissue by 3D cultures

Author

Adèle Arlat, Marie-Laure Renoud, Jean Nakhle, Miguel Thomas, Jessica Fontaine, Emmanuelle Arnaud, Cédric Dray, Hélène Authier, Paul Monsarrat, Agnès Coste, Louis Casteilla, Marielle Ousset, and Béatrice Cousin

Subjects
resident macrophage, macrophage subpopulation, adipose tissue, bone marrow, 3D culture, metabolism, Immunologic diseases. Allergy, RC581-607
Abstract

IntroductionWithin adipose tissue (AT), different macrophage subsets have been described, which played pivotal and specific roles in upholding tissue homeostasis under both physiological and pathological conditions. Nonetheless, studying resident macrophages in-vitro poses challenges, as the isolation process and the culture for extended periods can alter their inherent properties.MethodsStroma-vascular cells isolated from murine subcutaneous AT were seeded on ultra-low adherent plates in the presence of macrophage colony-stimulating factor. After 4 days of culture, the cells spontaneously aggregate to form spheroids. A week later, macrophages begin to spread out of the spheroid and adhere to the culture plate.ResultsThis innovative three-dimensional (3D) culture method enables the generation of functional mature macrophages that present distinct genic and phenotypic characteristics compared to bone marrow–derived macrophages. They also show specific metabolic activity and polarization in response to stimulation, but similar phagocytic capacity. Additionally, based on single-cell analysis, AT-macrophages generated in 3D culture mirror the phenotypic and functional traits of in-vivo AT resident macrophages.DiscussionOur study describes a 3D in-vitro system for generating and culturing functional AT-resident macrophages, without the need for cell sorting. This system thus stands as a valuable resource for exploring the differentiation and function of AT-macrophages in vitro in diverse physiological and pathological contexts.

Published
2024
Full Text
View/download PDF

2. Driving regeneration, instead of healing, in adult mammals: the decisive role of resident macrophages through efferocytosis

Author

Lise Rabiller, Virginie Robert, Adèle Arlat, Elodie Labit, Marielle Ousset, Marie Salon, Agnès Coste, Léa Da Costa-Fernandes, Paul Monsarrat, Bruno Ségui, Mireille André, Christophe Guissard, Marie-Laure Renoud, Marine Silva, Gilles Mithieux, Isabelle Raymond-Letron, Luc Pénicaud, Anne Lorsignol, Louis Casteilla, Cécile Dromard Berthézène, and Béatrice Cousin

Subjects
Medicine
Abstract

Abstract Tissue repair after lesion usually leads to scar healing and thus loss of function in adult mammals. In contrast, other adult vertebrates such as amphibians have the ability to regenerate and restore tissue homeostasis after lesion. Understanding the control of the repair outcome is thus a concerning challenge for regenerative medicine. We recently developed a model of induced tissue regeneration in adult mice allowing the comparison of the early steps of regenerative and scar healing processes. By using studies of gain and loss of function, specific cell depletion approaches, and hematopoietic chimeras we demonstrate here that tissue regeneration in adult mammals depends on an early and transient peak of granulocyte producing reactive oxygen species and an efficient efferocytosis specifically by tissue-resident macrophages. These findings highlight key and early cellular pathways able to drive tissue repair towards regeneration in adult mammals.

Published
2021
Full Text
View/download PDF

3. Driving regeneration, instead of healing, in adult mammals: the decisive role of resident macrophages through efferocytosis

Author

Anne Lorsignol, Isabelle Raymond-Letron, Léa Da Costa-Fernandes, Lise Rabiller, Béatrice Cousin, Marine Silva, Adèle Arlat, Elodie Labit, Paul Monsarrat, Agnès Coste, Bruno Ségui, Gilles Mithieux, Marie-Laure Renoud, Mireille André, Cécile Dromard Berthézène, Marie Salon, Luc Pénicaud, Virginie Robert, Marielle Ousset, Christophe Guissard, Louis Casteilla, STROMALab, Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Etablissement Français du Sang-Institut National de la Santé et de la Recherche Médicale (INSERM), McGill University = Université McGill [Montréal, Canada], Geroscience and rejuvenation research center (RESTORE), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Calgary, Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Institut de Recherche pour le Développement (IRD), Centre de Recherches en Cancérologie de Toulouse (CRCT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Nutrition, diabète et cerveau, Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Cell biology, Physiology, [SDV]Life Sciences [q-bio], Immunology, Biomedical Engineering, Medicine (miscellaneous), Granulocyte, Biology, Regenerative medicine, Article, Lesion, 03 medical and health sciences, 0302 clinical medicine, medicine, Efferocytosis, Tissue homeostasis, Loss function, 030304 developmental biology, 0303 health sciences, Regeneration (biology), 3. Good health, Haematopoiesis, medicine.anatomical_structure, Medicine, medicine.symptom, 030217 neurology & neurosurgery, Developmental Biology
Abstract

Tissue repair after lesion usually leads to scar healing and thus loss of function in adult mammals. In contrast, other adult vertebrates such as amphibians have the ability to regenerate and restore tissue homeostasis after lesion. Understanding the control of the repair outcome is thus a concerning challenge for regenerative medicine. We recently developed a model of induced tissue regeneration in adult mice allowing the comparison of the early steps of regenerative and scar healing processes. By using studies of gain and loss of function, specific cell depletion approaches, and hematopoietic chimeras we demonstrate here that tissue regeneration in adult mammals depends on an early and transient peak of granulocyte producing reactive oxygen species and an efficient efferocytosis specifically by tissue-resident macrophages. These findings highlight key and early cellular pathways able to drive tissue repair towards regeneration in adult mammals.

Published
2021
Full Text
View/download PDF

4. Identification of Adipose Tissue as a Reservoir of Macrophages after Acute Myocardial Infarction

Author

Ingrid Gomez, Virginie Robert, Paul Alayrac, Adèle Arlat, Vincent Duval, Marie-Laure Renoud, José Vilar, Mathilde Lemitre, Jean-Sébastien Silvestre, Béatrice Cousin, Cousin, Béatrice, Paris-Centre de Recherche Cardiovasculaire (PARCC (UMR_S 970/ U970)), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Geroscience and rejuvenation research center (RESTORE), Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Ventricular Remodeling, diabetes, macrophages, myocardial infarction, adipose tissue, Organic Chemistry, General Medicine, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Catalysis, Computer Science Applications, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Inorganic Chemistry, Mice, Inbred C57BL, Disease Models, Animal, Mice, Diabetes Mellitus, Type 2, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Animals, Physical and Theoretical Chemistry, Molecular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Spectroscopy
Abstract

International audience; Medullary and extra-medullary hematopoiesis has been shown to govern inflammatory cell infiltration and subsequently cardiac remodeling and function after acute myocardial infarction (MI). Emerging evidence positions adipose tissue (AT) as an alternative source of immune cell production. We, therefore, hypothesized that AT could act as a reservoir of inflammatory cells that participate in cardiac homeostasis after MI. To reveal the distinct role of inflammatory cells derived from AT or bone marrow (BM), chimeric mice were generated using standard repopulation assays. We showed that AMI increased the number of AT-derived macrophages in the cardiac tissue. These macrophages exhibit pro-inflammatory characteristics and their specific depletion improved cardiac function as well as decreased infarct size and interstitial fibrosis. We then reasoned that the alteration of AT-immune compartment in type 2 diabetes could, thus, contribute to defects in cardiac remodeling. However, in these conditions, myeloid cells recruited in the infarcted heart mainly originate from the BM, and AT was no longer used as a myeloid cell reservoir. Altogether, we showed here that a subpopulation of cardiac inflammatory macrophages emerges from myeloid cells of AT origin and plays a detrimental role in cardiac remodeling and function after MI. Diabetes abrogates the ability of AT-derived myeloid cells to populate the infarcted heart.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results