1. Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals
- Author
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Liliana Scorzoni, Maria José Soares Mendes-Giannini, Cassamo Ussemane Mussagy, Rondinelli Donizetti Herculano, Bruna Cambraia Garms, Monica Yonashiro Marcelino, Júlia Carina Niemeyer, Junya de Lacorte Singulani, Felipe Azevedo Borges, Nayrim Brizuela Guerra, Ana Marisa Fusco-Almeida, Giovana Sant’Ana Pegorin Brasil, Mateus Scontri, Francisco A.O. Carvalho, Universidade Estadual Paulista (UNESP), The University of Queensland, Universidade Federal de Santa Catarina (UFSC), University of Caxias do Sul (UCS), and Federal University of Southern and Southeastern Pará (UNIFESSPA)
- Subjects
Process Chemistry and Technology ,Borohydride ,Pollution ,Absorbance ,chemistry.chemical_compound ,Sodium borohydride ,chemistry ,Colloidal gold ,Toxicity ,Sodium citrate ,Chemical Engineering (miscellaneous) ,Gold nanoparticles ,Nanotoxicity ,Viability assay ,Avoidance response ,Cytotoxicity ,Waste Management and Disposal ,Alternative animal model ,Nuclear chemistry - Abstract
Made available in DSpace on 2022-04-29T08:36:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) In this work we synthesize and characterize AuNPs using two chemical routes: via sodium citrate and via sodium citrate/borohydride. The cytotoxicity of AuNPs was tested by traditional in vitro assays and the in vivo toxicity was evaluated using alternative animals (Galleria mellonella and Caenorhabditis elegans). Avoidance tests with Folsomia candida were carried out to evaluate possible toxic effects in the soil ecosystem. Through Scanning Electron Microscopy and Transmission Electron Microscopy we verified the spherical shape of the AuNPs, with diameters of 34.8 ± 5.5 nm (via sodium citrate) and 7.9 ± 2.2 nm (via sodium citrate/borohydride). The hydrodynamic diameters values were compatible with those obtained microscopically, 30.1 ± 10.1 nm (method A) and 8.3 ± 2.8 nm (method B). Stability of up to 6 months was verified for AuNPs synthesized via sodium citrate, while synthesis via citrate/sodium borohydride was stable for only 1 month. The AuNPs from method A showed better efficacy in the NPs formation with higher absorbance in the plasmonic band at 523 nm, higher concentration and size, spherical shape, homogeneity and stability. The cytotoxicity of the two AuNPs exhibited a slight dose- and size-dependent behavior, with cell viability greater than 80%. The in vivo toxicity in the G. mellonella model showed a survival rate of 100% for 7 days, however, with C. elegans, the percent of survival increased as the concentration of AuNPs decreased. All concentrations of AuNPs, except for the AuNPs synthesized by the citrate pathway (1 mg/kg), showed non-avoidance responses when exposed to contaminated soil. School of Pharmaceutical Sciences Department of Clinical Analysis São Paulo State University (UNESP) Institute of Chemistry Department of Biochemistry & Chemical Technology São Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Bioprocesses and Biotechnology Engineering São Paulo State University (UNESP) School of Chemistry and Molecular Biosciences The University of Queensland Postgraduate Program in Agricultural and Natural Ecosystems (PPGEAN) Federal University of Santa Catarina (UFSC) Area of Exact Sciences and Engineering University of Caxias do Sul (UCS) Institute of Exact Sciences Federal University of Southern and Southeastern Pará (UNIFESSPA) School of Pharmaceutical Sciences Department of Clinical Analysis São Paulo State University (UNESP) Institute of Chemistry Department of Biochemistry & Chemical Technology São Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Bioprocesses and Biotechnology Engineering São Paulo State University (UNESP) FAPESP: 2016/11836-0 FAPESP: 2017/19603-8 CNPq: 470261/2012-9
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- 2021