In order to make the rapid and large-scale propagation of fast-growing varieties of Pinus elliottii, the induction and proliferation of embryogenic callus were studied. The immature zygotic embryos (with endosperm) of ten P. elliottii families were used to systematically study the effects of different factors on the induction efficiency of embryogenic callus, including genotype, the developmental stage of zygotic embryos, basic medium, types and concentrations of plant growth regulator ( PGR) . At the same time, the proliferation conditions of embryogenic callus were discussed. The esults were as follows: The genotype, the developmental stage of zygotic embryos, basic medium and types and concentrations of plant growth regulator (PGR) all had different degrees of influence on the induction of embryogenic callus. Embryogenic callus were induced in all ten genotypes, of which the induction rate of Genotype 2 was the highest, reaching 25.37%; In the stage of zygotic embryo development, the induction rate of cones at the multiple embryo stage was the highest; Among the four basic media, the induction effect on DCR basic medium was the best; The induction rate of 2,4-D 2.0 mg L+KT 2.5 mg L-1 was the highest, reached 27.78%. The proliferation of embryogenie callus for P. elliottii was achieved on DCR medium, supplemented with 2,4-D (0.5 mg L-1), KT (1.0 mg - L-1), CH (500 mg L) and Gin (300 mg L-1). This study laid a foundation for further developing mature embryo induction and plant regeneration of fast-growing varieties of P. elliottii. [ABSTRACT FROM AUTHOR]