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The biodegradable composite material was prepared by injection molding with PHBV as matrix and microcrystalline cellulose and waste paper fiber as reinforcements. The iffects of MCC with different mass fraction and waste paper fiber with different mass fraction on the mechanica properties, water absorption and interfacial properties of the composites were investigated. The results show that the mechanical properties of the composites after adding MCC have been improved and the mechanical properties of the composites are the best when the mass fraction of MCC is 3%. And the composite material after blending and filling has the best combination mechanical properties when the mass fraction of waste paper is 10%. The water absorbency of composites increases with the increase of fiber content in waste paper. Scanning electron microscopy analysis shows that the compatibility of MCC with P-HBV interface is better and the interfacial properties of blended waste paper fibers decrease. [ABSTRACT FROM AUTHOR]

Objective: CX3CL1 played an important role in atherosclerosis recently. We investigated the association between the chemokine CX3CL1 with coronary artery disease in type 2 diabetes mellitus. Methods: CX3CL1 expression in carotid artery specimens was analysed by immunohistochemistry. The study was conducted in 400 coronary artery disease in type 2 diabetes mellitus and 400 control subjects in a case-control study. CX3CL1 gene rs170364 variant was detected by the directing sequencing technology. Results: CX3CL1 expressions were increased markedly in carotid artery plaque than in normal carotid artery. CX3CL1 gene rs170364 genotype (GG, GT and TT) frequency in coronary artery disease in type 2 diabetes mellitus is 42.7%, 40.0% and 17.2%, and 50.2%, 39.6% and 10.2% in the control group. CX3CL1 gene rs170364 variant is a genetic risk factor for coronary artery disease in type 2 diabetes mellitus (P <0.05). Logistic regression analysis with adjustments for other risk factors showed that the CX3CL1 gene rs170364 T allele significantly increases the risk of coronary artery disease in type 2 diabetes mellitus compared with the control subjects (P <0.05). Conclusion: Our study shows that the CX3CL1 expression increased in the carotid plaques and serum of the coronary artery disease in type 2 diabetes mellitus. CX3CL1 gene rs170364 variant may be considered a genetic risk factor for coronary artery disease in type 2diabetes mellitus. [ABSTRACT FROM AUTHOR]

Objective: To explore the clinical significance of serum CX3CL1 level for coronary artery disease. Methods: The study was conducted in 250 cases of coronary artery disease patients(75 patients with stable angina pectoria,75 patients with unstable angina pectoris,50 patients with ST-elevation acute myocardial infarction,50 non ST-elevation acute myocardial infarction patients) and 200 cases of control subjects(normal coronay artery) in a case-control study. The CX3CL1 level in different groups were analyzed by ELISA and CX3CR1 expression on CD4~+CD28~-T cells was analyzed by flow cytometry. Results: The serum CX3CL1 level increased markedly in coronary artery disease patients compared with the control subjects(P<0.05). The serum CX3CL1 level of patients with stable unstable angina was increased in the patients with ST-elevation acute myocardial infarction than those with the non ST-elevation acute myocardial infarction patients(P<0.05). An increased number of CD4~+CD28~-CX3CR1+T cells was found in the patients with coronary artery disease patients than the control group especially in the ST-elevation acute myocardial infarction patient(P<0.05). Conclusion: CX3CL1 might be involved in the formation of coronary atherosclerotic plaque and played an important role in the atherosclerotic procession,which might be useful to predict the stability of plaque in the early stage of coronary artery disease. [ABSTRACT FROM AUTHOR]

Objective: To explore the clinical significance of serum CX3CL1 level for coronary artery disease. Methods: The study was conducted in 250 cases of coronary artery disease patients (75 patients with stable angina pectoria, 75 patients with unstable angina pectoris, 50 patients with ST-elevation acute myocardial infarction, 50 non ST-elevation acute myocardial infarction patients) and 200 cases of control subjects (normal coronay artery) in a case-control study. The CX3CL1 level in different groups were analyzed by ELISA and CX3CR1 expression on CD4+CD28-T cells was analyzed by flow cytometry. Results: The serum CX3CL1 level increased markedly in coronary artery disease patients compared with the control subjects (P<0.05). The serum CX3CL1 level of patients with stable unstable angina was increased in the patients with ST-elevation acute myocardial infarction than those with the non ST-elevation acute myocardial infarction patients (P<0.05). An increased number of CD4+CD28-CX3CR1+T cells was found in the patients with coronary artery disease patients than the control group especially in the ST-elevation acute myocardial infarction patient (P<0.05). Conclusion: CX3CL1 might be involved in the formation of coronary atherosclerotic plaque and played an important role in the atherosclerotic procession, which might be useful to predict the stability of plaque in the early stage of coronary artery disease. [ABSTRACT FROM AUTHOR]

Objective: To study the expressions of chemokine CCL2 in the plasma and the platelets of patients with STEMI, and investigate the effect of CCL2 on P65 and IκBα of the platelets. Methods: 50 patients with STEMI who were admitted in the CCU of General Hospital of Shenyang Military Region from October 2015 to January 2016 and 50 healthy controls were enrolled. The plasma CCL2 concentration of patients with STEMI and healthy controls were detected by ELISA. The expressions of CCL2 and CCR2 in the platelets were detected by western blot; the phosphorylation of P65 and IκBα in the platelets were tested by western blot after CCL2 stimulation, or while CCR2 antagonist RS201895 and NF-κB signaling pathway inhibitor Bayl 1-7082 were pretreated on the platelets. Results: The plasma CCL2 concentration of STEMI patients and healthy controls were 222± 98 pg/mL and 162± 24 pg/mL, there were significant statistical differences between the two groups (P＜0.01). Compared with control group, the expression of CCL2 and CCR2 in the platelets were significantly increased in STEMI patients (P＜0.01). After the platelets were stimulated by CCL2, pP65 increased and IκBα decreased (P＜0.01); when RS201895 or Bay 11-7082 treated before CCL2 stimulation, the phosphorylation of P65 decreased and IκBα increased (P＜0.01). Conclusions: The expression of CCL2 in plasma and platelets of patients with STEMI increased. CCL2/CCR2 might affect the functions of platelets through NF-κB signaling pathway and participate in the thrombosis. [ABSTRACT FROM AUTHOR]

Objective: To investigate the association between the ACE gene ID variant with coronary artery disease (CAD) in Northern Han population of China. Methods: In this study we used the gene directing sequencing analysis to detect the ACE gene I/D variant among the 56S CAD patients and 529 control subjects by a case-control study. Results: hi CAD patients and controls subjects, we found that ACE gene ID variant conformed to the Hardy-Weinberg equilibrium well. ACE gene ID variant (II. ID and DD(genotype frequency was 50.3 %. 30.2 % and 19.5 % in CAD patients and 41.7 %. 47.2 % and 11.1 % in controls subjects. ACE gene ID variant maybe a genetic risk factor for CAD in Chinese Han population (P< 0.05). Logistic regression analysis with adjustments for the other risk factors of CAD showed that ACE gene I/D variant significantly increased the risk of CAD compared with the non-carriers (P<0.05). Conclusions: hi Northern Han population of China. ACE gene ID variant maybe considered a genetic risk factor for CAD. [ABSTRACT FROM AUTHOR]

Objective: To investigate the association between the interleukin 23 Receptor (IL23R) rs6682925 variant with coronary artery disease (CAD) in Northern Han population of China. Methods: The study was conducted in 568 coronary artery disease patients and 529 control subjects in a case-control study. We used the gene directing sequencing technology to detect the IL23R rs6682925 and rs1884444 variant. Results: We found that the IL23R rs6682925 and rs1884444 variant frequency conformed well to the Hardy-Weinberg equilibrium in both case and control subject. IL23R rs6682925 (CC, TC and TT) genotype frequency was 22.9 %, 39.6 % and 37.5 % in CAD group and 41.7 %, 47.2 % and 11.1 % in control group. IL23 R rs6682925 C allele was a genetic risk factor for coronary artery disease in Northern Han population of China (P<0.05). There were no significant differences in the genotype and allele distribution of IL23R rs1884444 variant between CAD and control subjects (P>0.05). Logistic regression analysis with adjustments for the other risk factors showed that the C allele carriers of IL23R rs6682925 variant significantly increased the risk of coronary artery disease compared with the non-carriers (P<0.05). Conclusions: IL23R rs6682925 variant may be considered a genetic risk factor for CAD in Northern Han population of China. [ABSTRACT FROM AUTHOR]

Materials with different infiltration conditions on the solid surface are called wettability material. According to different wettability, the material plays a great role in the daily life and industrial production. The preparation idea is to construct the surface roughness and reduce surface energy. At present, the trend of material development is to preserve the inherent properties of structural materials, obtain low surface wettability, and also give some special material function to broaden the scope of their application. In this paper, the research progress of the preparation, properties and application based on inorganic and organic materials at home and abroad was reviewed, and research situation and application prospect of wetting surface material aim at stimulus response, such as temperature, light, magnetic and dielectric, multiple response were further summed up. The limitation and problems of the development of low wetting surface materials were discussed. The development prospect of functional responsive wetting materials was prospected. [ABSTRACT FROM AUTHOR]

Objective: To observe the effect of C-C motif ligand2 (CCL2) on the expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs cells were isolated and cultured in vitro. HUVECs were plated into 6-well plate, when cells were grown to 80-90 %, CCL2 overexpression plasmid [pcDNA3.1 (+)-CCL2] or CCL2 small interfering RNA (si-CCL2) was transfected into HUVECs. At different time points after transfection (12 h, 24 h and 48 h), HUVECs were collected for RNA and protein extraction. The mRNA expressions of CCL2 and ICAM-1 were examined using quantitative RT-PCR. The protein expressions of CCL2 and ICAM-1 were examined using western blotting. Results: (1) Compared with pcDNA3.1 (+) group, the expressions of CCL2 mRNA and protein were significantly higher in pcDNA3.1 (+)-CCL2 group; Compared with si-Control group, the expressions of CCL2 mRNA and protein were significantly decreased in si-CCL2 group. (2) Compared with pcDNA3.1 (+) group, pcDNA3.1 (+)-CCL2 significantly increased the mRNA and protein expressions of ICAM-1 in HUVECs, and si-CCL2 significantly inhibited the mRNA and protein expressions of ICAM-1 in HUVECs compared with si-Control group. Conclusions: CCL2 could affect ICAM-1 expression in HUVECs, which may provide a theoretical basis for clarifying the mechanisms of atherosclerosis. [ABSTRACT FROM AUTHOR]

In order to reduce the use of toxic organic solvents？ environmentally friendly water-soluble superhydrophobic materials were prepared. Waterborne epoxy resiri poly(glycidyl methacry 1 ate-random butyl acrylater andom-hydroxy et h y 1 meth aery late (「(GMA-r-ftA-r-HEM八))was prepared by radical polymerization heptafluorobutyric acid (HFBA) and riano-silica modified hybrid assembly, using water as solvent to prepare superhydrophobic materials, and superhydrophobic surface on cotton fabric surface. The hydrophobic properties and solvent resistance of cotton fabrics were investigated by changing the content of amino silica. The results show that the superhydrophobic effect of superhydrophobic treated cotton fabrics was better with the increase of amino silica content when the amount of grafted fluorine was constant. The cotton fabric impregnated with the super hyd rophobic material had a good lyophobic effect ？ the water contact angle was (1 50zh2)°, and the durability time was 83 niiri. It had good solvent resistance and could withstand water washing, ultrasonic and NaCO solution washing. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship between serum CC chemokine ligand 5 (CCL5) level and the severity of coronary atherosclerosis. Methods: The expression of CCL5 protein in carotid atherosclerotic plaques obtained from carotid eversion endarterectomy (CEE) of 5 cases of patients with highgrade stenosis (>70 %) (AS group) and 3 cases of normal postmortem arteries (control group) were detected by Immunohistochemistry and Western blotting. The CCL5 expression was compared between the two groups. 110 cases of consecutive patients who underwent coronary angiography were selected and divided into the control group (Gensini's score = 0, n=27), mild lesions group (020, n=51). The blood pressure, blood lipid, blood glucose and serum CCL5 of all groups were investigated. Their serum CCL5 levels were measured by enzyme linked immunosorbent assay (ELISA). The relationship between risk factors, serum CCL5 level and Gensini score were analyzed. Results: The expression of CCL5 protein in the carotid artery atherosclerosis was evidently higher than that in the normal carotid artery, and the serum CCL5 level of CAD patients was significantly higher than that of the control group. Sex, smoking history, systolic pressure, diastolic pressure, glucose and serumCCL5 were significantly different among three groups. Correlation analysis indicated that diastolic pressure, glucose and serum CCL5 were positively correlated with Gensini's score (r=0.276, 0.418, 0.519; P<0.05). In multi-variant linear regression model, serum CCL5 (B=8.775; P=0.000) were established as independent predictors of Gensini's score. Conclusion: Serum CCL5 was positively correlated with the severity of coronary artery atherosclerosis and was proved to be an independent predictor of Gensini's score. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship between serum CC chemokine ligand 5 (CCL5) level and the severity of coronary atherosclerosis. Methods: The expression of CCL5 protein in carotid atherosclerotic plaques obtained from carotid eversion endarterectomy (CEE) of 5 cases of patients with highgrade stenosis (>70 %) (AS group) and 3 cases of normal postmortem arteries (control group) were detected by Immunohistochemistry and Western blotting. The CCL5 expression was compared between the two groups. 110 cases of consecutive patients who underwent coronary angiography were selected and divided into the control group (Gensini's score = 0, n=27), mild lesions group (020, n=51). The blood pressure, blood lipid, blood glucose and serum CCL5 of all groups were investigated. Their serum CCL5 levels were measured by enzyme linked immunosorbent assay (ELISA). The relationship between risk factors, serum CCL5 level and Gensini score were analyzed. Results: The expression of CCL5 protein in the carotid artery atherosclerosis was evidently higher than that in the normal carotid artery, and the serum CCL5 level of CAD patients was significantly higher than that of the control group. Sex, smoking history, systolic pressure, diastolic pressure, glucose and serum CCL5 were significantly different among three groups. Correlation analysis indicated that diastolic pressure, glucose and serum CCL5 were positively correlated with Gensini's score (r=0.276, 0.418, 0.519; P<0.05). In multi-variant linear regression model, serum CCL5 (B=8.775; P=0.000) were established as independent predictors of Gensini's score. Conclusions: Serum CCL5 was positively correlated with the severity of coronary artery atherosclerosis and was proved to be an independent predictor of Gensini's score. [ABSTRACT FROM AUTHOR]