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This paper presents the results of one of the hydrological models, the InVEST "Annual Water Yield" (InVEST–AWY), applied to the Meta River basin in Colombia, which covers an area of 113,981 km². The study evaluates the performance of the model in different subbasins of the Meta River basin. The model's accuracy was assessed using different statistical measures, including Nash–Sutcliffe Efficiency (NSE) coefficient, Root Mean Square Error (RMSE), correlation coefficients for the calibration (rcal) and validation (rval) periods. The overall performance of the model in the Meta River basin is relatively poor as indicated by the low NSE value of 0.07 and high RMSE value of 1071.61. In addition, the model explains only a 7% of the variance in the observed data. The sensitivity analysis revealed that a 30% reduction in crop coefficient (Kc) values would result in a 10.7% decrease in water yield. The model estimated, for example, the annual average water yield of the river in 2018 as 1.98 × 1011 m3/year or 6273.4 m3/s, which is 1.3% lower than the reported value. The upper Meta River subbasin shows the highest NSE value (0.49), indicating a good result between observed and simulated water discharge. In contrast, the South Cravo River subbasin shows a negative NSE value of −1.29, indicating poor model performance. The Yucao River subbasin and the upper Casanare River subbasin also show lower NSE values compared to the upper Meta River subbasin, indicating less accurate model performance in these subbasins. The correlation coefficients in calibration (rcal) and validation (rval) for the upper Meta River, Yucao River, South Cravo River, and upper Casanare River subbasins were 0.79 and 0.83, 0.4 and 0.22, 0.5 and −0.25, and 0 and 0.18, respectively. These results provide useful insights into the limitations for the proper use of the InVEST–AWY model in Colombia. This study is the first to use the InVEST–AWY model on a large scale in the territory of Colombia, allowing to evaluate its effectiveness in hydrological modeling for water management. [ABSTRACT FROM AUTHOR]