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Germination of seeds is a complex process linked to various molecular and biochemical changes that increase the nutritional value. This research has studied the impact of germination on the enzymatic and non-enzymatic antioxidant activities of five domestic staple legumes from Pakistan: chickpea, mung bean, soybean, lentil, and peanut. The maximum moisture content was recorded for chickpea, i.e., 11.7 ± 0.05 g 100 g−1 followed by lentil, soybean, peanut, and mung bean as 9.65 ± 0.02, 8.07 ± 0.01, 7.6 ± 0.01, and 7.31 ± 0.01 g 100 g−1, respectively. The overall augmentation in catalase activity during germination (days 1 to 4) was maximum for lentils, i.e., 76.22%, and ascorbate peroxidase activity for peanuts, i.e., 62.09%. The soybean showed a clear difference from the 1st to 4th days of germination for peroxide activity among all selected legumes with a 37.29% increase. A maximum change in superoxide dismutase was noticed in mung bean. The mung bean also had the highest variation in total flavonoid contents, while total phenolic contents were higher in the chickpea. The radical scavenging activity varied higher in peanuts, while the ascorbic acid content was maximum for soybeans. Among all selected legumes, non-germinated chickpeas showed a lower DPPH value, i.e., 1.15 ± 0.03%, whereas the highest DPPH value was observed in lentils (69.84 ± 0.05%). Thus, the incorporation of ready-to-eat (RTE) germinated legumes with high antioxidant activity would be a contemporary addition to the everyday meal. [ABSTRACT FROM AUTHOR]

Soft drinks are widely consumed worldwide; therefore, it is necessary to study their qualitative and quantitative analysis. For this purpose, we studied 18 brands of soft drinks purchased from the local market of Bahawalpur, Pakistan. Carbon dioxide, glucose, sucrose, ascorbic acid, phosphates, caffeine, and alcohol were analyzed qualitatively, while pH, conductivity, density, potassium, sodium, and total soluble solids were estimated quantitatively. This physico-chemical analysis was determined by the Association of Official Analytical Chemists (AOAC) methods. The obtained results demonstrated that these soft drinks were highly acidic, ranging from 2.3±0.01 to 3.68±0.01 due to carbon dioxide and phosphates. Most soft drinks contain traces of caffeine and alcohol, while very few contain ascorbic acid. The conductivity of all the soft drinks was high due to soluble ions. Similarly, density was higher than water due to a large amount of sugar. The concentration of total soluble solids, mainly known as sucrose, was also high, ranges 64±0.01 g/L (2.56±0.01 Brix°) to 126.5±0.02 g/L (21.57±0.02 Brix°). Most cold drinks contain some amount of sodium (0.3±0.01 to 3.0±0.01 mg/L), potassium (0±0.00 to 12.8±0.01 mg/L), and a small amount of calcium (0.1±0.01 to 0.53±0.01 mg/L), the potassium concentration was higher than the sodium. In contrast, calcium concentration is very small. The strength of acid was estimated quantitatively using a titrimetric method, the maximum concertation of acid is 20.92±0.01. Similarly, using redox titration, alcohol concentration was measured, ranges 0.46±0.01 to 0±0.00, but this concentration was less than WHO's concentration, therefore considered safe to use. [ABSTRACT FROM AUTHOR]

The current study was conducted at Post Harvest Research Centre, Ayub Agricultural Research Institute, Faisalabad, Pakistan in 2018-2019 to investigate the postharvest application of two antioxidants i.e. ascorbic acid (AA) and benzyl adenine (BA) on physicochemical properties and shelf life extension of guava fruit (Psidium guajava L.). The application of AA was done @ of 500, 750 and 1000 ppm, and BA was applied @ of 25, 50 and 75 ppm along with control for ten minutes and stored at 25 ± 2°C with a relative humidity of (70-75%). During storage, various physicochemical parameters were assessed at a three-day interval to the maximum acceptable period. Among various treatments, the BA @ 75 ppm was found effective to retain the lowest physiological weight loss (5.19%), greater firmness (1.95 kg/cm2 ) and TSS (6.95 Brix), ascorbic acid (82.66 mg/100g) and reducing sugar (3.83%). In addition, the total phenolic contents (0.15%), chlorophyll contents (0.21%) and pectin (0.32%) were found to be higher in BA treated fruit (75 ppm) as compared to control. The outcomes noticeably validate that 75 ppm application of BA showed a greater potential to enhance the shelf life and retain the quality characteristics of guava fruit. [ABSTRACT FROM AUTHOR]

Present experiment was performed at fruit orchard of Horticultural Research Station, Bahawalpur during 2016-18 to explore optimum concentration of urea causing deblossoming and defoliation in summer crop, subsequently improving fruit quality and production in winter crop of guava cv. ‘Surahi’. Five treatment combinations containing different concentrations of urea applications viz 0% (T0 =control), 09% (T1 ), 12% (T2 ), 15% (T3 ) and 18% (T4 ) which were applied twice fortnightly when summer crop attained 50% blooming. Percentages of flower drop, fruit drop, fruit set and defoliation as well as fruit weight, fruit length & diameter, total soluble solids, acidity, total sugars, ascorbic acid and yield per plant were recorded. Significant differences among treatment combinations were recorded in effect on each parameter during both years of study. Results from combined analysis of two-years data indicated that 15% urea spray on summer crop produced the highest yield (64.83 kg/plant), heavier fruit (203 g), bigger fruit size (length 9.3 cm & diameter 7.5cm), higher TSS (15o Brix), total sugars (7.1%) with minimum acidity (0.65%) in winter crop because of higher flower (75.5%) and fruit drop (41.5%), defoliation (25.8%) and less fruit set (17.7%) compared to non-target summer crop. High concentration of urea (18%) ranked 2nd with respect to fruit and yield parameters of winter crop but it led in flower dropping (80%), fruit dropping (46.8%), defoliation of summer crop (33%) and ascorbic acid (164.4 mg/100g pulp). Urea spray @ 15% proved the best for deblossomig and defoliation of summer crop. [ABSTRACT FROM AUTHOR]

The experiment was conducted at Biochemistry Section, Ayub Agricultural Research Institute, Faisalabad Pakistan, during the year 2017-18 to assess the total phenols and antioxidants potential of mango, grapes, banana, guava, mulberry, strawberry, loquat, melon, apricot and phalsa. Fruits samples were obtained from the market of Faisalabad Pakistan, washed, dry at room temperature, extracted their juice/pulp. Thejuice was stored at 4 °C for further analysis. Juice samples were processed for Vitamin-C, pH, TSS, total phenolics and total antioxidant capacity. The results showed that among all the ten fruits vitamin C was higher in mango (24.33 mg/100 mL) followed by phalsa (19.74 mg/100 mL) and Grapes (11.25 mg/100 mL), whereas lower value was observed in melon (3.3 mg/100 mL) and loquat (3.4 mg/100 mL). Vitamin C for other fruits lies between these. Highly acidic pH of fresh juices of all the fruits was observed except in melon (5.82) and guava (5.38) which is less acidic. Percentage of TSS was higher in phalsa, mulberry and mangoes (13.8, 13.5 and 13.9% respectively) and lesser in melon (5.6%). Antioxidant capacity (% DPPH reduction) was found higher in grapes (91.9%) and mango (91.2%) which was statistically at par with melon (90.9%), while lesser value was observed in mulberry (51.6%). Total phenolics were significantly higher in grapes (1266.4 pg GAE/mL), whereas minimum phenolic contents were found in melon (4.1 pg GAE/mL). Therefore, it was concluded that grapes are more nutritious and healthy fruit than other. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the clinical efficacy of Ascorbic acid in relieving pain and inflammation following surgical removal of the third molar. Study Design: Quasi-experimental study. Place and Duration of Study: Armed Forces Institute of Dentistry, Rawalpindi Pakistan, from Jan to Mar 2023. Methodology: Sixty participants who required surgical extraction of the impacted third molar were included and segregated equally into two groups. Group-A received Amoxicillin with Clavulanic acid (625 mg) thrice a day and Metronidazole (400 mg) twice daily, while Group-B received Amoxicillin with Clavulanic acid (625 mg) thrice daily, Ascorbic acid (500 mg) twice daily and Metronidazole (400 mg) twice daily. Both the groups received Naproxen Sodium as per requirement (550 mg). Pain, trismus, facial swelling, and requirement of analgesic consumption were evaluated on the seventh post-operative day. Results: There was a statistically significant difference in reducing the intensity of pain (p<0.01), severity of facial swelling and trismus (p<0.01) and requirement of analgesic consumption between Group A and B (p<0.001). Conclusion: Our study concluded that Ascorbic acid after surgery effectively reduces pain intensity, the severity of facial swelling and trismus, and the requirement for analgesic consumption so that ascorbic acid may be used as an adjuvant therapy with other conventional drugs. [ABSTRACT FROM AUTHOR]

Poor seed germination is a crucial yield-limiting factor when winter wheat is sown under low temperature. The objective of this study was to evaluate the role of ascorbic acid (AsA) in the extenuation of the harmful effects of low temperature at early and reproductive stages of wheat during 2016–2017 (15 November to 15 December). A two-year experiment was conducted using a randomized complete block design with split plot arrangement and with three replicates. Sowing dates (15 November and 15 December) were allotted to the main plot while seed priming (control, hydro-priming, and AsA priming) were allotted to the sub-plot. Results demonstrated that AsA priming significantly boosted different yield characteristics including chlorophyll content, tillers per unit area, number of grains per spike, and 1000-grain weight, contributing higher productivity and biomass during 2016–2017. The results further revealed that AsA could induce the up-regulation of diverse antioxidants (super oxide dismutase (SOD), peroxidase (POD), and catalase (CAT)), thus offsetting the adverse effects of sub-supra optimum temperatures of late sowing wheat. It is therefore concluded in this work that AsA priming enhances stand establishment, yield and yield-related traits, antioxidant enzyme activities, and chlorophyll contents when wheat is sown under low temperature. [ABSTRACT FROM AUTHOR]