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Objectives: To evaluate whether the glycosylation of chrysin (CHR) enhances its protective effects against aluminum-induced neurotoxicity. Methods: To compare the antioxidant, anticholinesterase, and behavioral effects of CHR with its glycosylated form (CHR bonded to β-d-glucose tetraacetate, denoted as LQFM280), we employed an integrated approach using both in vitro (SH-SY5Y cells) and in vivo (aluminum-induced neurotoxicity in Swiss mice) models. Key findings: LQFM280 demonstrated higher antioxidant activity than CHR in both models. Specifically, LQFM280 exhibited the ability to exert antioxidant effects in the cytoplasm of SH-SY5Y cells, indicating its competence in traversing neuronal membranes. Remarkably, LQFM280 proved more effective than CHR in recovering memory loss and counteracting neuronal death in the aluminum chloride mice model, suggesting its increased bioavailability at the brain level. Conclusions: The glycosylation of CHR with β-d-glucose tetraacetate amplifies its neuroprotective effects, positioning LQFM280 as a promising lead compound for safeguarding against neurodegenerative processes involving oxidative stress. Graphical Abstract [ABSTRACT FROM AUTHOR]

Conductive polymers can stimulate the adhesion and proliferation of several cell types being an interesting material to be applied in the field of tissue engineering. In this application, the addition of a thermosensitive polymer has been pointed out as an attractive strategy to improve mechanical strength and to provide conformation changes at temperature close to human body temperature. The main goal of this work was to evaluate the physical–chemical properties and promotion of cell proliferation and possible application as scaffolds for tissue engineering of blends of poly(N‐vinylcaprolactam) (PNVCL) and poly(N‐vinylcaprolactam‐co‐butylacrylate) (PNVCL‐co‐ABu) with poly(3‐hexylthiophene) (P3HT). Both polymers were synthesized and their chemical structures were confirmed by Fourier Transform Infrared (FT‐IR) and nuclear magnetic resonance (NMR) analyses. The materials were processed in films form for structural characterization, impedance measurements, and thermogravimetric analysis. The in vitro biological assays were performed using the materials as electrospun mats. The FT‐IR was also used to characterize the blends. The addition of P3HT did not change thermal stability and showed low conductivity values of the blends. Comparing the in vitro behavior of the blends and the polymers it was possible to conclude that the presence of P3HT improved cytocompatibility and promoted cell proliferation. [ABSTRACT FROM AUTHOR]

This work describes the isolation and structural elucidation of compounds from the leaves of Myrcia tomentosa (Aubl.) DC. (goiaba-brava) and evaluates the antimicrobial activity of the crude extract, fractions and isolated compounds against bacteria and fungi. Column chromatography was used to fractionate and purify the extract of the M. tomentosa leaves and the chemical structures of the compounds were determined using spectroscopic techniques. The antibacterial and antifungal activities were assessed using the broth microdilution method. The phytochemical investigation isolated 11 compounds: α-bisabolol, α-bisabolol oxide B, α-cadinol, β-sitosterol, n-pentacosane, n-tetracosane, quercetin, kaempferol, avicularin, juglanin and guaijaverin. The crude ethanolic extract and its fractions were tested against 15 bacteria and 9 yeasts. The crude extract inhibited the in vitro growth of yeasts at concentration of 4 to 32 μg/mL. The hexane, dichloromethane, ethyl acetate and aqueous fractions inhibited Candida sp. at concentrations of 4 to 256 μg/mL, whereas the Cryptococcus sp. isolates were inhibited only by the hexane and dichloromethane fractions in minimal inhibitory concentrations (MICs) at 16 to 64 μg/mL. The flavonoid quercetin-3-O-α-arabinofuranose (avicularin) was the most active compound, inhibiting Candida species in concentrations of 2 to 32 μg/mL. The MIC values suggest potential activity of this plant species against yeast. [ABSTRACT FROM AUTHOR]