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Hog insurance and rural environmental protection are complementary to each other. Studying the environmental effects of hog insurance is imperative for safeguarding food safety and promoting the long-term development of the agricultural insurance industry. Informed by the risk management theory and sustainable development theory, this paper constructs a theoretical framework for the impact of policy-supported hog insurance on the green total factor productivity (GTFP) of hog farming. Utilizing panel data from China's hog-dominant production areas spanning from 2005 to 2021, the slacks-based measures of directional distance functions (SBM-DDF) model and multiple-time-point difference-in-differences (DID) approach were used to measure GTFP and explore the effects of hog insurance on GTFP and the underlying mechanisms. The findings indicate a substantial enhancement in GTFP due to hog insurance. The conclusion drawn was robust to various tests. The mechanism is that hog insurance fosters GTFP by expanding the breeding scale, adjusting the planting–breeding structure, and promoting technological progress. Furthermore, the environmental effects of hog insurance policy are more pronounced in economically developed regions, with significant effects observed on the GTFP of free-range, small-scale, and medium-scale hog-farming households. This study contributes new evidence to the field of assessing the environmental impact of agricultural insurance policies and provides valuable insights for furthering green transformation and development in the hog insurance-supported breeding industry. [ABSTRACT FROM AUTHOR]

Weather radar plays an important role in monitoring aerial animal migration, providing a stable data source for biological studies with large-scale coverage and consecutive-time samples. The accurate estimation of bird density from weather radar echoes is fundamental for quantitative biological studies. We analyzed the bird observation model in weather radar, and proposed a method to build the bird quantification model by jointly utilizing dual-polarization Doppler weather radar and scanning bird radar. We designed a detailed process to remove tracks or echoes from non-bird targets, ensuring the effectiveness of bird observations. The field experiments validated the quantification method, showing that the average radar cross section of birds in Jinan was 19.09 dBscm (i.e., 81.19 cm2; 95% confidence interval, CI: 18.92–19.27 dBscm) for the S-band weather radar, with an R2 of 0.79. In addition, through the correlation analysis, we found that the ground terrain may affect the distribution pattern of aerial bird density. [ABSTRACT FROM AUTHOR]

The problem of high grain production costs, which is not conducive to sustainable agricultural development and food security, is highlighted in the context of China's "large country and small household farmers". Reducing the grain production costs through factor allocation and organizational drive has become particularly important. Based on 768-grain peasant households in China, this paper uses OLS regression and robust regression to examine the effects of agricultural factor inputs and cooperatives on grain production costs. It analyzes the synergistic and substitution effects between farmers' factor inputs and cooperatives in grain production. It was found that: (1) in farmers' grain production, reductions in the grain production costs can be realized by expanding the area under cultivation, improving the use of agricultural machinery, and increasing technological inputs; (2) a reduction in the grain production costs can also be realized through cooperatives driving farmers into grain production; (3) cooperatives can provide farmers with various types of agricultural production services in grain production and cooperative-driven substitution effects between the agricultural factor inputs of farm households. The findings of this paper contribute to the enrichment of research in the field of agricultural production and are important for enhancing agricultural sustainability and reducing grain production costs. [ABSTRACT FROM AUTHOR]

Promoting farmers' participation in rural industrial integration and driving farmers' agricultural production with cooperatives and agribusinesses are conducive to realizing cost saving, efficiency, and green production and guaranteeing food security and sustainable agricultural development. Based on the microsurvey data of 1039 grain farmers in Henan Province, China in 2022, this paper examined the impact of contractual choices of farmers' participation in rural industrial integration on agricultural green productivity while analyzing the mechanism of action by using OLS regression, a causal mediation analysis of instrumental variables, propensity score matching, and two-stage least squares (2SLS). The study found that: (1) farmers' participation in a contract, driven by cooperatives or agribusinesses to carry out agricultural production, is conducive to improving their agricultural green productivity, but the effect of each main body to drive farmers varies; (2) farmers' participation in a contract, through cooperatives or agribusinesses to obtain all kinds of agricultural production services—such as agricultural machinery services, agricultural supply services, and technical guidance services—improves the use of agricultural machinery, the standardization of chemical fertilizers, pesticides, and other agricultural materials' use, increases technical guidance, and improves agricultural green productivity. The findings of this paper suggest policy and practical implications for safeguarding food security and promoting sustainable agriculture, as well as enriching research on agricultural productivity. [ABSTRACT FROM AUTHOR]

This study addresses the problem of restricted ability for large-scale monitoring due to the limited cruising time of unmanned aerial vehicles (UAV) by identifying an optimal leaf ChlorophyII content (LCC) inversion machine learning model at different scales and under different parameterization schemes based on simultaneous observations of ground sampling, UAV flight, and satellite imagery. The following results emerged: (1) The correlation coefficient between most remote sensing features (RSFs) and LCC increased as the remote scale expanded; thus, the scale error caused by the random position difference between GPS and measuring equipment should be considered in field sampling observations. (2) The LCC simulation accuracy of the UAV multi-spectral camera using four machine learning algorithms was ExtraTree > GradientBoost > AdaBoost > RandomForest, and the 20- and 30-pixel scales had better accuracy than the 10-pixel scale, while the accuracy for three feature combination schemes ranked combination of extremely significantly correlated RSFs > combination of significantly correlated and above RSFs > combination of all features. ExtraTree was confirmed as the optimal model with the feature combination of scheme 2 at the 20-pixel scale. (3) Of the Sentinel-2 RSFs, 27 of 28 were extremely significantly correlated with LCC, while original band reflectance was negatively correlated, and VIs were positively correlated. (4) The LCC simulation accuracy of the four machine learning algorithms ranked as ExtraTree > GradientBoost > RandomForest > AdaBoost. In a comparison of two parameterization schemes, scheme 1 had better accuracy, while ExtraTree was the best algorithm, with 11 band reflectance as input RSFs; the RMSE values for the training and testing data sets of 0.7213 and 1.7198, respectively. [ABSTRACT FROM AUTHOR]

Each year, billions of birds migrate across the globe, and interpretation of weather radar signals is increasingly being used to document the spatial and temporal migration patterns in Europe and America. Such approaches are yet to be applied in the East Asian‐Australasian Flyway (EAAF), one of the most species‐rich and threatened flyways in the world. Logistical challenges limit direct on‐ground monitoring of migratory birds in many parts of the EAAF, resulting in knowledge gaps on population status and site use that limit evidence‐based conservation planning. Weather radar data have great potential for achieving comprehensive migratory bird monitoring along the EAAF. In this study, we discuss the feasibility and challenges of using weather radar to complement on‐ground bird migration surveys in the flyway. We summarize the location, capacity and data availability of weather radars across EAAF countries, as well as the spatial coverage of the radars with respect to migrants' geographic distribution and migration hotspots along the flyway, with an exemplar analysis of biological movement patterns extracted from Chinese weather radars. There are more than 430 weather radars in EAAF countries, covering on average half of bird species' passage and non‐breeding distributions, as well as 70% of internationally important sites for migratory shorebirds. We conclude that the weather radar network could be a powerful resource for monitoring bird movements over the full annual cycle throughout much of the EAAF, providing estimates of migration traffic rates, site use, and long‐term population trends, especially in remote and less‐surveyed regions. Analyses of weather radar data would complement existing ornithological surveys and help understand the past and present status of the avian community in a highly threatened flyway. [ABSTRACT FROM AUTHOR]

The purpose of this study is to understand the relationship between social capital and the performance of Farmers' Cooperatives (Cooperatives) and explore the internal mechanism of social capital affecting the performance of Cooperatives. This work selects two dimensions: cognitive social capital (CSC) and structural social capital (SSC), as indexes to measure the social capital of Cooperatives. An analytical framework is proposed: "Social capital-Dynamic capabilities-Organizational performance." First, according to the characteristics of Cooperatives, it determines the most appropriate index values and preprocesses the original data. Statistical Product and Service Solutions (SPSS) and Analysis of Moment Structure (AMOS) 25.0 software are used for factor analysis. A financial performance evaluation model of Cooperatives based on backpropagation neural network (BPNN) is constructed. Then, based on the survey data of 212 Cooperatives in Liaoning Province, the structural equation model (SEM) is used to test the interaction path between "Social capital-Dynamic capacity-Organizational performance." The results show that SSC's standardized regression coefficients (SRCs) on Cooperatives' economic benefits and member satisfaction are 0.208 and 0.095, respectively, significant at 1%. The actual case analysis concludes that the larger the scale of the structural network embedded in Cooperatives is, the more conducive it is to obtaining extensive resources. As such, Cooperatives can absorb the advanced experience and compensate for the weakness of lack of internal resources and experience. The SRC of CSC on Cooperatives' economic benefits is 0.336, and the P value is 0.204, indicating an insignificant impact of CSC on Cooperatives' economic benefits. This work considers environmental variability, uses dynamic capacity as an independent variable, opens the "black box" between social capital and the performance of Cooperatives, and reveals the intermediate path between the two. [ABSTRACT FROM AUTHOR]

• Combining ICESat-2 and GEE can monitor water volume change in large areas precisely. • Precision of lake volume change is mainly affected by water level measurement. • ICESat-2 laser altimetry data has high accuracy in estimating water level change. • Seasonal variations in water level of natural lake and reservoir vary significantly. • Reservoirs generally show larger variations than lakes in water volume change. Monitoring the water level and volume changes of lakes and reservoirs is essential for deepening our understanding of the temporal and spatial dynamics of water resources in the Yellow River Basin, with a view to better utilizing and managing water resources. In recent years, there have been many studies on monitoring water level and volume changes in inland waters, but they were mainly focused on radar altimetry and the full waveform LiDAR ICESat, which was retired in 2010. Few studies based on the latest photon-counting LiDAR ICESat-2 have been reported. Compared with previous sensors, ICESat-2 has great advantages in footprint size, transmitting frequency, pulse number, etc, but its performance in monitoring water level and volume changes in inland waters has not been fully explored. Here we investigated the spatial distribution of water level and volume changes of 11 lakes and 8 reservoirs in the Yellow River Basin based on ICESat-2 and Google Earth Engine, and analyzed the factors affecting the measurement uncertainties. In-situ validation of lake level in Lake Qinghai indicates that the Root Mean Square Error (RMSE) of our result is only 7 cm after the reference coordinate system conversion. We found that the water level trend of the natural lake shows significant seasonal variations, while the water level trend of the reservoir shows a sharp rise and fall. In addition, precipitation plays a decisive role in the changes in natural lake levels and indirectly affects the artificial control of reservoirs' water discharges. The uncertainty of water volume change monitoring is mainly affected by water level measurement uncertainty for lakes, while for reservoirs, that is affected by the combination of water level and area measurement uncertainties. The stability of lake level measurement increases with the increase in photon counts. The introduction of ICESat-2 ATL13 Significant Wave Height might lead larger standard deviation in water level measurement. According to the law of propagation of uncertainty, the uncertainty of the water volume change estimation by the combination of ICESat-2 and GEE is less than 9 %. [ABSTRACT FROM AUTHOR]

Reuse of reclaimed water in constructed wetlands is a promising way to conserve water resources and improve water quality, and it is playing a very important role in wetland restoration and reconstruction. This study utilized reflectance spectra of wetland vegetation to estimate nitrogen content in water in the Beijing Bai River constructed wetland, a typically constructed wetland that uses reclaimed water. Canopy reflectance spectra of two dominant plants in the wetland, including reed and cattail, were acquired using a spectrometer (350–2500 nm). Simultaneously, water samples were collected to measure water quality. To establish the appreciate relationship between total nitrogen content (TN) and reflectance spectra, both simple and multiple regression models, including simple ration spectral index (SR), normalized difference spectral index (ND), stepwise multiple linear regression (SMLR) model, and partial least squares regression (PLSR), were adopted in this study. The results showed that (1) compared with simple regression models (SR and ND), multiple regressions models (SMLR and PLSR) could provide a more accurate estimation of TN concentration in the wetland environment. Among these models, the PLSR model had the highest accuracy and was proven to be the most useful tool to reveal the relationship between the spectral reflectance of wetland plants and the total nitrogen consistency of wetland at the canopy scale. (2) The inversion effect of TN concentration in water is slightly better than that of wetland vegetation, and the reflection spectrum of the reed can predict TN concentration more accurately than that of cattail. The finding not only provides solid evidence for the potential application of remote sensing to detect water eutrophication but also enhances our understanding of the monitoring and management of water quality in urban wetlands using recycled water. [ABSTRACT FROM AUTHOR]

Industry convergence is the future trend of industrial development in rural areas and is conducive to high-quality agriculture development. To explore the development dynamics of industry convergence. This paper selects data from 31 provincial administrative regions in China from 2009 to 2019. It uses the entropy power method to measure the development quality of rural industrial integration in China and empirically studies the impact of transportation infrastructure on rural industrial integration using a spatial panel autoregressive model. The study found that: (1) from 2009–2019, the development quality of rural industrial integration is on the rise, but the development is uneven between regions; (2) transport infrastructure strongly promotes the development of rural industrial integration; (3) with the help of transport infrastructure, rural industrial integration in this region will improve the quality of rural industrial integration in the surrounding areas; and (4) the impact of transportation facilities varies in different regions and at different stages of development of rural industrial integration. The results of this paper are beneficial to improving transportation infrastructure planning and exploring the driving force of high-quality agriculture development, enriching the research of spatial land use, and providing valuable insights for developing industry convergence in other countries and regions. [ABSTRACT FROM AUTHOR]

The trajectory planning of UAV with nonholonomic constraints is usually taken as differential algebraic equation to solve the optimal control problem of functional extremum under the condition of inequality constraints. However, it can be challenging to meet the requirements of real-time for the high complexity. A differential flat theory based on B-spline trajectory planning can replace the optimal control problem with nonlinear programming and be a good means to achieve the efficient trajectory planning of an UAV under multiple dynamic constraints. This research verifies the feasibility of this theory with actual flight experiments. [ABSTRACT FROM AUTHOR]

Three Microwave Radiation Imagers (MWRI) were carried onboard the FengYun-3B/C/D satellites and have collected more than 10 years of data since 2010. To create a robust climate quality of data, MWRI level one data were reprocessed with new calibration. This study evaluates the performance of retrieving global soil moisture from recalibrated MWRI data (RCD) and quantifies the difference of retrieved soil moisture between operational calibration data (OCD) and RCD. Soil Moisture Operational Products System (SMOPS) products from NOAA on four days of different seasons were collocated with MWRI brightness temperatures, and then the collocated data were used for training an algorithm through machine learning. The retrieved soil moisture products using OCD and RCD were evaluated against the independent SMOPS products, in situ networks and SMAP soil moisture product. It is shown that the algorithm from the random forest is suitable for FY-3D recalibrated MWRI data, with a coefficient of determination (R2) of 0.7223, a mean bias of −0.0062 and an unbiased root mean square difference (ubRMSD) of 0.0476 m3 m−3 compared with SMOPS products over the period from 12 July 2018 to 31 December 2019. The difference of retrieved soil moisture using OCD and RCD is spatially heterogeneous. Both temporal and spatial coverage and accuracy of the existing FY-3D operational soil moisture products are significantly improved. [ABSTRACT FROM AUTHOR]

Teaching reflection based on videos is the main method in teacher education and professional development. However, it takes a long time to analyse videos, and teachers are easy to fall into the state of information overload. With the development of "AI + education," automatic recognition of teacher behavior to support teaching reflection has become an important research topic. In this paper, taking online open classroom teaching video as the data source, we collected and constructed a teacher behavior dataset. Using this dataset, we explored the behavior recognition methods based on RGB video and skeleton information, and the information fusion between them is carried out to improve the recognition accuracy. The experimental results show that the fusion of RGB information and skeleton information can improve the recognition accuracy, and the early-fusion effect is better than the late-fusion effect. This study helps to solve the problems of time-consumption and information overload in teaching reflection and then helps teachers to optimize the teaching strategies and improve the teaching efficiency. [ABSTRACT FROM AUTHOR]

Large-scale insect migration may cause severe plant diseases and pests which can exert serious impact on agriculture. Predicting the trajectory and destination of insect migration accurately is an effective way to prevent pests. Here, an insect migration trajectory simulation method was proposed with two stages of insect migration, taking off and cruising, considered. First, four criteria based on actual weather condition were proposed to determine the take-off area of the insect migration. Then, a method based on Lagrange diffusion model was proposed to simulate the cruising trajectory. This step needs to combine with the insect orientation strategy and insect trajectory speed which can be, respectively, provided by entomological radar and weather radar. Finally, by comparing the determined take-off area and the simulated insect cruising trajectory with the monitoring results of the weather radar, the effectiveness of the method was verified. [ABSTRACT FROM AUTHOR]

The weather radar can operate in all weathers and all time, and has a large coverage area. Besides monitoring the weather, the weather radar can receive other echoes including biological echoes. In order to utilise weather radar biological monitoring capability, recognising and classifying local insect and bird echoes is one of the biggest obstacles for analysing their migration, foraging, and reproduction activities. Here, a pixel-wise classification method based on the fully convolutional network (FCN) is proposed which is trained by the radar reflectivity and the spectral width images. Moreover, to increase the biometric detection accuracy, the region growing method is combined for achieving the region edge alignment. Finally, the proposed method is validated based on the real weather radar datasets in Yantai. The FCN training results have a high pixel accuracy of 92.96%, and the region growing method performs well in the edge alignment. [ABSTRACT FROM AUTHOR]

Weather radars provide continuous recording over extensive spatial coverage, which is valuable for biologists who observe and study biological activities over a wide range of temporal and spatial scales. Through the interpretation of weather radar observations, powerful biological inferences can be obtained. However, when it comes to certain biological problems, such as the determination of biological parameters related to airborne biological densities, weather radar data needs to be processed based on certain assumptions before. This article analyses and calculates the impact of the phenomenon of migratory insects gathering into layers on the interpretation of weather radar data and develops a biomass estimation method with known bio-spatial distribution characteristics. Quantitative research was conducted on the situation of migrating insects across the Bohai Bay in the autumn of 2012. [ABSTRACT FROM AUTHOR]

Microbial cell surface display has attracted greater attention than ever and has numerous potential applications in biotechnology. With the safety and probiotic properties, lactic acid bacteria (LAB) have been used widely in food and industrial applications. In order to circumvent using genetically modified microorganisms which face low public acceptance and severe regulatory scrutiny, surface-engineered LAB without genetical modification are more preferred. According to the way used to obtain the fusion protein containing the passenger molecule and anchoring domain, the genetic or chemical approaches can be used to construct these surface-engineered LAB. In addition to the viable wide-type LAB, non-living bacterial-like particles (BLP) can be attached by these fusion proteins added from outside. Compared to the living LAB, BLP have a higher binding capacity and less anticarrier response. Mucosal vaccines are the predominant application of these surface-engineered LAB with no genetical modification. [ABSTRACT FROM AUTHOR]

Weather radar data can capture large-scale bird migration information, helping solve a series of migratory ecological problems. However, extracting and identifying bird information from weather radar data remains one of the challenges of radar aeroecology. In recent years, deep learning was applied to the field of radar data processing and proved to be an effective strategy. This paper describes a deep learning method for extracting biological target echoes from weather radar images. This model uses a two-stream CNN (Atrous-Gated CNN) architecture to generate fine-scale predictions by combining the key modules such as squeeze-and-excitation (SE), and atrous spatial pyramid pooling (ASPP). The SE block can enhance the attention on the feature map, while ASPP block can expand the receptive field, helping the network understand the global shape information. The experiments show that in the typical historical data of China next generation weather radar (CINRAD), the precision of the network in identifying biological targets reaches up to 99.6%. Our network can cope with complex weather conditions, realizing long-term and automated monitoring of weather radar data to extract biological target information and provide feasible technical support for bird migration research. [ABSTRACT FROM AUTHOR]

Soil moisture (SM) products derived from passive satellite missions are playing an increasingly important role in agricultural applications, especially crop monitoring and disaster warning. Evaluating the dependability of satellite-derived soil moisture products on a large scale is crucial. In this study, we assessed the level 2 (L2) SM product from the Chinese Fengyun-3C (FY-3C) radiometer against in-situ measurements collected from the Chinese Automatic Soil Moisture Observation Stations (CASMOS) during a one-year period from 1 January 2016 to 31 December 2016 across Henan in China. In contrast, we also investigated the skill of the Advanced Microwave Scanning Radiometer 2 (AMSR2) and Soil Moisture Active/Passive (SMAP) SM products simultaneously. Four statistical parameters were used to evaluate these products' reliability: mean difference, root-mean-square error (RMSE), unbiased RMSE (ubRMSE), and the correlation coefficient. Our assessment results revealed that the FY-3C L2 SM product generally showed a poor correlation with the in-situ SM data from CASMOS on both temporal and spatial scales. The AMSR2 L3 SM product of JAXA (Japan Aerospace Exploration Agency) algorithm had a similar level of skill as FY-3C in the study area. The SMAP L3 SM product outperformed the FY-3C temporally but showed lower performance in capturing the SM spatial variation. A time-series analysis indicated that the correlations and estimated error varied systematically through the growing periods of the key crops in our study area. FY-3C L2 SM data tended to overestimate soil moisture during May, August, and September when the crops reached maximum vegetation density and tended to underestimate the soil moisture content during the rest of the year. The comparison between the statistical parameters and the ground vegetation water content (VWC) further showed that the FY-3C SM product performed much better under a low VWC condition (<0.3 kg/m2) than a high VWC condition (>0.3 kg/m2), and the performance generally decreased with increased VWC. To improve the accuracy of the FY-3C SM product, an improved algorithm that can better characterize the variations of the ground VWC should be applied in the future. [ABSTRACT FROM AUTHOR]

Abstract The low-low temperature electrostatic precipitator (LLT-ESP) is one of the most used devices for pollutant control in ultra-low emission coal-fired power plants. This study investigated the influence of the LLT-ESP on polycyclic aromatic hydrocarbons (PAHs) distributions in flue gas from an ultra-low emission coal-fired power plant. The total gas-phase PAH concentration was reduced from 27.52 μg/m3 to 3.38 μg/m3. The total particulate-phase PAH concentration decreased from 14.36 μg/m3 to 0.34 μg/m3. The removal efficiency of the LLT-ESP for gas-phase and particulate phase carcinogenic higher molecular weight (HMW) PAHs was 85% and 99%, respectively. The total concentration of 16 selected PAHs in feed coal was 98.16 μg/g. The fly ash particle size successively decreased from Electric Field 1 (F1) to Electric Field 4 (F4). The total PAH concentration decreased from F1 to F2 but increased again from F3 to F4. The flue gas cooling process significantly contributed to the elimination of both gas- and particulate-phase PAHs in the flue gas. Presumably, most of the condensed PAHs were adhered to or absorbed in the fly ash and were scavenged in Field 1. Both gas- and particulate-phase 5- and 6-ring PAHs in the flue gas were completely removed in Field 1. The discharge process in the electric fields may promote the formation of several 4- or 5-ring PAHs. In this study, benzo[k]fluoranthene (BKF) and benzo[a]pyrene (BaP) were regenerated in the particles rather than in the flue gas during the discharge process in the electric fields. Graphical abstract Image 1 Highlights • The low-low temperature electrostatic precipitator has effective removal effect on polycyclic aromatic hydrocarbons (PAHs). • The flue gas cooling process significantly contributed to the elimination of both gas- and particulate-phase PAHs in flue gas. • A few 4-ring or 5-ring PAHs may have regenerated in the particles due to the discharge process in the electric fields. [ABSTRACT FROM AUTHOR]

Oxidative stress in aging has attracted much attention; however, the role of reductive stress in aging remains largely unknown. Here, we report that the endoplasmic reticulum (ER) undergoes reductive stress during replicative senescence, as shown by specific glutathione and H 2 O 2 fluorescent probes. We constructed an ER-specific reductive stress cell model by ER-specific catalase overexpression and observed accelerated senescent phenotypes accompanied by disrupted proteostasis and a compromised ER unfolded protein response (UPR). Mechanistically, S -nitrosation of the pivotal ER sulfhydryl oxidase Ero1α led to decreased activity, therefore resulting in reductive stress in the ER. Inhibition of inducible nitric oxide synthase decreased the level of Ero1α S -nitrosation and decreased cellular senescence. Moreover, the expression of constitutively active Ero1α restored an oxidizing state in the ER and successfully rescued the senescent phenotypes. Our results uncover a new mechanism of senescence promoted by ER reductive stress and provide proof-of-concept that maintaining the oxidizing power of the ER and organelle-specific precision redox regulation could be valuable future geroprotective strategies. [Display omitted] • ER reductive stress promotes cellular senescence. • ER proteostasis and ER UPR capacity are compromised under ER reductive stress. • Inactivation of Ero1α by S -nitrosation during senescence leads to ER reductive stress. • Specific up-regulation of ER oxidizing power is a new geroprotective strategy. [ABSTRACT FROM AUTHOR]

• Single chain insulin (SCI) is delivered by bacteria bacterium like particles (BLPs). • Oral vaccination with BLPs-SCI-59 efficiently induces. antigen-specific tolerance. • Oral vaccination with BLPs-SCI-59 efficiently prevents diabetes in NOD mice. • BLPs may be used as an antigen delivery vehicle to induce immune tolerance. Oral tolerance, induced by oral administration of autoantigens, is a promising therapeutic approach to treat type 1 diabetes mellitus (T1DM). However, the degradation of antigens passing through the gastrointestinal tract (GIT) leads to low induction efficiency. Based on our previous study, a single-chain insulin (SCI-59) analog, bound to the surface of lactic acid bacteria (LAB) bacterium-like particles (BLPs), was more stable in the simulated gastric fluid, compared to free SCI-59 and insulin. Based on the analysis of diabetes progression, a significant decrease in the incidence of diabetes was observed in mice fed BLPs-SCI-59. Oral administration of BLPs-SCI-59 can enhance glucose tolerance in NOD mice and this effect may result from the protection of pancreatic islet beta cells, as compared to the free SCI-59 group and BLPs group. Oral administration of BLPs-SCI-59 can significantly reduce insulitis and preserve the ability of insulin secretion in treated mice. Oral vaccination with BLPs-SCI-59 induced SCI-59 specific T cell tolerance in treated mice, which may due to the repair of Th1/Th2 imbalance and increased CD4+CD25+FoxP3+ regulatory T cells (Tregs). These results show that oral vaccination with BLPs-SCI-59 is a promising way to prevent T1DM in NOD mice. [ABSTRACT FROM AUTHOR]