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As rising sea levels alter coastal ecosystems, there is a pressing need to examine the effects of saltwater intrusion on coastal communities. Using 16S Illumina profiling, we characterized the communities of baldcypress tree (Taxodium distichum) root endosphere and rhizosphere soil bacteria. Our study utilized established sites along salinity and flooding gradients in the United States of Georgia, Louisiana, and South Carolina. We hypothesized that environmental variables, namely salinity and water level, as well as distance between sites would be correlated with baldcypress-associated rhizosphere and root endosphere bacterial communities. We found that geographic distance correlated with rhizosphere but not root endosphere bacterial communities, suggesting that the trees may have stabilized their endosphere communities via recruitment of a more specific suite of taxa from the surrounding soil. Mean water level, mean salinity, and the volume of woody debris were associated with both endosphere and rhizosphere bacterial communities in baldcypress trees. The density of host trees was also associated with endosphere community composition. Our study is the first to use 16S Illumina sequencing to characterize bacterial communities in baldcypress trees— a key restoration species in coastal swamp ecosystems under threat from rising sea levels.

Decomposing foliar litter organic matter (LOM) in forested watersheds is an essential terrestrial source of dissolved organic matter (DOM) to aquatic ecosystems. To evaluate the relationship of chemical composition between LOM and DOM, we collected freshly fallen leaves of white oak (Quercus alba) and conducted an 80-week field decomposition experiment along a small elevation gradient in a floodplain within the Congaree National Park, South Carolina. Foliar litters were collected for water extraction and instrumental analyses using pyrolysis GC/MS, UV/VIS and fluorescence spectrophotometry. Factor analyses of pyrolysates showed that fresh LOM was mainly composed of lignin compounds (LgC), phenolic compounds (PhC), and carbohydrate (Carb), and the change in composition was relatively small throughout the decomposition process. In contrast, there were two distinct chemical compositions of DOM in early (between 0–8 weeks) and late phases (between 16–80 weeks). The early phase had a higher percentage of PhC, but the late phase contained higher percentages of Carb, unsaturated hydrocarbon (UnSaH), aromatic hydrocarbon (ArH), and nitrogen-containing compounds (Ntg). The fluorescence emission-excitation matrix showed there was an increasing trend in humic and fulvic-like fractions in DOM over time, matching well with increases of UnSaH and ArH fractions from the results of pyrolysis GC/MS. Our study illustrated that the changes of chemical components in LOM and water extractable DOM were not parallel during the decomposition process and the degradation of lignin and phenolic compounds was one of the controlling factors on the production of DOM.

Occupancy patterns of herpetofauna in most tidal freshwater swamps are unknown. Tidal freshwater swamps currently face multiple threats, including salinization, which can influence their associated plant and animal communities. The impacts of salinization to herpetofauna communities in tidal freshwater swamps have not been assessed. To improve predictions regarding these herpetofauna, we conducted surveys in tidal freshwater swamps of the Savannah National Wildlife Refuge located in South Carolina, USA, from March to June, 2016 and 2017, using a variety of methods. Goals included inventorying species, determining communities, examining microhabitat associations, and modeling occupancy to predict the impacts of salinity changes. We detected 8 species of amphibians and 12 species of reptiles in our surveys. Community analyses failed to detect patterns related to measured environmental variables. Species richness and diversity declined along the salinity gradient, but the observed patterns did not match our predictions and may instead be related to site-level heterogeneity. Microhabitat associations were detected for two amphibian species via occupancy analyses. Occupancy and regression analyses indicated soil salinity may be a factor affecting nine species’ occurrences. Amphibian detections may be affected by water depth, pH values, and weather conditions. These results expand our understanding of herpetofauna within an understudied, and threatened, wetland type.

Riverine forests support high rates of plant productivity, yet total wood carbon (C) stocks in these systems remain understudied. We measured C concentrations and dry mass of live and dead detrital wood to understand their importance relative to total ecosystem C storage across an elevation gradient on the floodplain of the Congaree River. Our study sites included a low elevation bald cypress swamp and three higher elevation mature mixed bottomland hardwood communities. Average wood C concentration was 45.9 ± 0.07% and estimated cumulative C pools (sum of downed and standing dead wood plus live tree, sapling, and shrub wood) ranged from 6850 to 17,200 g C/m2 for bottomland hardwood forests and 17,700 g C/m2 for the bald cypress swamp. Most of the aboveground C was stored in living wood (83.1–90.5% total aboveground pools). Our results indicate that the mass and C stored in live and dead wood are at the high end of both measured and modeled ranges reported in the literature for temperate zone forests. As such, the mature bottomland forests of the Congaree River represent a substantial and important store of sequestered C.

Nowadays forest fires are so rare in the Mississippi Alluvial Valley and other floodplains of the southeastern USA that these floodplains appear fireproof. Fire was once much more common across the Southeastern Coastal Plain, including in these forested floodplains. Even so, fire was not the fundamental ecological disturbance in floodplain forests that it was in adjacent uplands; flooding served that role here. Other disturbances, like hurricanes, tornados, and beavers opened floodplain forests and may have been antecedents to fires. Since the arrival of Europeans, the disturbance regime in perhaps no other ecoregion in the continental USA has been so fundamentally altered. Levee- and dam-building have radically changed the hydrology of most of these river floodplains. Logging, agricultural conversion, urbanization, and road-building have further altered ecosystem structure and function. Fire is exceedingly rare in modern, closed-canopy floodplain forests, where fuels remain moist year-round, well-drained sites are converted from canebrakes to crop fields, and fires that once burned across adjacent uplands are either halted by habitat fragmentation or actively suppressed. This chapter will discuss the role of fire in floodplains of the southeastern USA in the context of other ecological and human-caused disturbances historically, presently, and looking to the future.

Plant roots assemble in two distinct microbial compartments: the rhizosphere (microbes in soil surrounding roots) and the endosphere (microbes within roots). Our knowledge of fungal community assembly in these compartments is limited, especially in wetlands. We tested the hypothesis that biotic factors would have direct effects on rhizosphere and endosphere assembly, while abiotic factors would have direct and indirect effects. Using a field study, we examined the influences of salinity, water level and biotic factors on baldcypress (Taxodium distichum) fungal communities. We found that endosphere fungi, unlike rhizosphere fungi, were correlated with host density and canopy cover, suggesting that hosts can impose selective filters on fungi colonizing their roots. Meanwhile, local abiotic conditions strongly influenced both rhizosphere and endosphere diversity in opposite patterns, e.g. highest endosphere diversity (hump-shaped) and lowest rhizosphere diversity (U-shaped) at intermediate salinity levels. These results indicate that the assembly and structure of the root endosphere and rhizosphere within a host can be shaped by different processes. Our results also highlight the importance of assessing how environmental changes affect plant and plant-associated fungal communities in wetland ecosystems where saltwater intrusion and sea level rise are major threats to both plant and fungal communities.

With rising sea levels, mortality of glycophytes can be caused by water and nutrient stress under increasing salinity. However, the relative effects of these two stressors may vary by species-specific functional traits. For example, deciduous species, with leaves typically emerging during low salinity periods of the year, may suffer less from water stress than evergreen species. We sampled two woody species with contrasting functional traits: the evergreen and N2-fixing waxmyrtle (Morella cerifera), and the deciduous and non-N2 fixing baldcypress (Taxodium distichum) along a coastal river (South Carolina, USA) showing an increasing pattern of plant mortality along a salinity gradient. We first analyzed oxygen and hydrogen isotope ratios of plant stem water and river water to determine changes in plant source water at different sites. Then we analyzed foliar carbon and nitrogen isotope ratios (δ13C and δ15N) along with nitrogen and phosphorus content (%N and %P) as proxies for the water and nutrient stress. Results showed that: (1) the two species had different water sources at the higher salinity sites; (2) foliar δ15N values of baldcypress decreased with higher salinity while retaining a constant δ13C value, and both of these isotope values were positively related with foliar %P, suggesting greater nutrient stress but minor water stress under high salinity; and (3) foliar δ13C values of waxmyrtle increased with higher salinity while retaining a constant foliar δ15N value, and neither of the values was significantly related to foliar nutrients, suggesting greater water stress but minor nutrient stress under high salinity. The different responses of the two species to high salinity may be related to their differences in leaf phenology and N2-fixation. Our results suggest that nutrient stress, particularly of P, can contribute to stress and eventual high mortality of baldcypress exposed to salt water intrusion.

Pyrolysis gas chromatography/mass spectrometry is a potent tool for studying the molecular fingerprints of natural organic matter (NOM). With advances in analytical techniques, a pyrogram generally consists over a hundred pyrolysates, which increases the difficulty of interpreting the associated data. Here, we propose a systematic approach that includes an automatic peak identification and quantification pipeline and statistical techniques for the analysis of NOM. White oak (Quercus alba) and forest floor litter samples from a 48-week field decomposition study including sites along a soil moisture gradient were used to evaluate the applicability. An analysis of variance of the chemical classes indicated that the composition differed among sites, although a trend following the moisture gradient was not observed. Factor analysis of the pyrolysates clearly identified two decomposition stages in both types of decomposition. For the oak litter, 2,6-dimethoxy-phenol originating from syringyl lignin was dominant in the early stage, whereas for the forest floor litter, 2-methoxy-4-vinylphenol was enriched in the early stage, while 4-ethyl-2-methoxyphenol and 3-allyl-6-methoxyphenol were dominant in the later stage. These compounds originated from guaiacyl lignin, which suggested that guaiacyl lignin was relatively constant. The proposed approach provides a convenient and effective way to study the chemical composition of NOM.

Forested wetlands dominated by baldcypress (Taxodium distichum) and water tupelo (Nyssa aquatica) are commonly found in coastal regions of the southeastern United States. Global climate change and in particular sea level rise will alter the frequency and magnitude of wet/dry periods and salinity levels in these ecosystems. Soil microcosm experiments were set up to identify the effects of water level variations (0.4–3.0 g-water g-soil−1) and salinity changes (0, 1 and 5 ppt of NaCl) on greenhouse gas emissions (CH4, CO2, and N2O) and dissolved organic carbon (DOC) characteristics from forested wetland soils. Our results indicate that, the effect of water level was much greater than salt intrusion on C and N cycling. Wet–dry treatments significantly decreased DOC production and total CH4-C loss, aromatic and humic-like substance compounds in DOC were increased in both flooding and wet–dry treatments after 60-d incubation. The molecular weight (MW) of DOC, as indicated by E2/E3 ratio and spectral slope, after flooding treatments was higher than that in wet–dry treatments. A first order kinetic model showed there was a positive linear correlation (r2 = 0.73) between CO2 emission rate and DOC concentration which indicated that CO2 was mainly generated from DOC. An exponential kinetic model was applied to describe the correlation between CH4 emission rate and DOC concentration (r2 = 0.41). This study demonstrates that an increase in salinity, and in particular variations in wet–dry cycles, will lead to changes in the formation of climate-relevant greenhouse gases, such as CH4, CO2, and N2O.

Tidal freshwater forested wetlands (TFFW) of the southeastern United States are experiencing increased saltwater intrusion mainly due to sea-level rise. Inter-annual and intra-annual variability in forest productivity along a salinity gradient was studied on established sites. Aboveground net primary productivity (ANPP) of trees was monitored from 2013 to 2015 on three sites within a baldcypress ( Taxodium distichum ) swamp forest ecosystem in Strawberry Swamp on Hobcaw Barony, Georgetown County, South Carolina. Paired plots (20 × 25-m) were established along a water salinity gradient (0.8, 2.6, 4.6 PSU). Salinity was continuously monitored, litterfall was measured monthly, and growth of overstory trees ⩾10 cm diameter at breast height (DBH) was monitored on an annual basis. Annual litterfall and stem wood growth were summed to estimate ANPP. The DBH of live and dead individuals of understory shrubs were measured to calculate density, basal area (BA), and important values (IV). Freshwater forest communities clearly differed in composition, structure, tree size, BA, and productivity across the salinity gradient. The higher salinity plots had decreased numbers of tree species, density, and BA. Higher salinity reduced average ANPP. The dominant tree species and their relative densities did not change along the salinity gradient, but the dominance of the primary tree species differed with increasing salinity. Baldcypress was the predominant tree species with highest density, DBH, BA, IV, and contribution to total ANPP on all sites. Mean growth rate of baldcypress trees decreased with increasing salinity, but exhibited the greatest growth among all tree species. While the overall number of shrub species decreased with increasing salinity, wax myrtle ( Morella cerifera ) density, DBH, BA, and IV increased with salinity. With rising sea level and increasing salinity levels, low regeneration of baldcypress, and the invasion of wax myrtle, typical successional patterns in TFFW and forest health are likely to change in the future.

Deepwater swamps, primarily baldcypress-water tupelo, pondcypress-swamp tupelo, or Atlantic white-cedar, are freshwater systems with standing water for most or all of the year. Probably the first classification of deepwater swamp forests was Shaler, who used both physical and vegetational characteristics to identify two types of forested wetlands: freshwater swamps and estuarine swamps. Other classification systems developed in the 1950s were based on vegetation, habitat, and the quantity, depth, and duration of water as diagnostic criteria. Deepwater swamps occur in a wide variety of geomorphic situations ranging from broad, flat floodplains to isolated basins. Soils of deepwater swamps generally have ample nutrients. Major flows of nutrients most frequently measured in these swamps include decomposition, wood accumulation, sedimentation, and return of nutrients from the forest canopy as litterfall. Fire is generally infrequent in natural deepwater forests of the southern United States because of the continuously moist conditions.

Tidally influenced coastal forested wetlands can be divided into two broad categories, mangroves and freshwater forested wetlands. These forested wetlands perform valuable ecosystem services, and both are endangered by threats of sea level rise and land use. Understanding the mechanisms that control the distribution of tidal forests has been greatly enhanced by innovation in measurement and modeling of tidal forcing brought about by satellite observation of sea level. Oceanographic hydrodynamic models can now be merged with riverine hydraulic models to address forcing functions in the upper estuary and tidal river. There are new opportunities to study these unique forested ecosystems in a context of (a) the physical driving mechanisms that control their distribution and (b) the anthropogenic and natural disturbances that impact these ecosystems. Remote sensing and geographic information system technology and hydrodynamic, hydraulic, and hydrologic modeling can and must be combined to understand the functioning of these dynamic systems and their interactions with the environment. This chapter summarizes the tidal process and ecosystem characteristics of tidal forested wetlands, with examples from eastern China and the Southeastern United States. The first example demonstrates the need for hydrodynamic modeling to correctly interpret a time series of satellite images in order to evaluate the impact of human management on tidal wetlands. The second examines both empirical data on tidal dynamics and geospatial modeling to examine effects of sea level rise on freshwater forested wetlands. A short review of two widely used large-scale hydrologic models is also provided for describing the flow transport in intertidal rivers, a transition between tidal estuaries and freshwater nontidal wetlands.

Here we review an extensive series of studies of Barataria Basin, an economically and ecologically important coastal basin of the Mississippi Delta. Human activity has greatly altered the hydrology of the basin by decreasing riverine inflows from leveeing of the river and its distributaries, increasing runoff with high nutrient concentrations from agricultural fields, and channelization of wetlands of the basin interior that has altered flow paths to often bypass wetlands. This has resulted in degraded water quality in the upper basin and wetland loss in the lower basin. Trophic state analysis found the upper basin to be eutrophic and the lower basin to be mesotrophic. Gross aquatic primary production (GAPP) was highest in the upper basin, lowest in the mid basin, and intermediate in the lower basin. Forested wetlands in the upper basin have degraded over the past several decades due to increased periods of flooding, while there has been massive loss of emergent wetlands in the lower basin due to increasing water levels and pervasive alteration of hydrology. Restoration will entail reconnection of waterways with surrounding wetlands in the upper basin, and implementation of river sediment diversions, marsh creation using dredged sediments and barrier island restoration. Findings from this review are discussed in terms of the functioning of deltas globally.

The objectives of this study were to identify processes that contribute to resilience of coastal wetlands subject to rising sea levels and to determine whether the relative contribution of these processes varies across different wetland community types. We assessed the resilience of wetlands to sea-level rise along a transitional gradient from tidal freshwater forested wetland (TFFW) to marsh by measuring processes controlling wetland elevation. We found that, over 5 years of measurement, TFFWs were resilient, although some marginally, and oligohaline marshes exhibited robust resilience to sea-level rise. We identified fundamental differences in how resilience is maintained across wetland community types, which have important implications for management activities that aim to restore or conserve resilient systems. We showed that the relative importance of surface and subsurface processes in controlling wetland surface elevation change differed between TFFWs and oligohaline marshes. The marshes had significantly higher rates of surface accretion than the TFFWs, and in the marshes, surface accretion was the primary contributor to elevation change. In contrast, elevation change in TFFWs was more heavily influenced by subsurface processes, such as root zone expansion or compaction, which played an important role in determining resilience of TFFWs to rising sea level. When root zone contributions were removed statistically from comparisons between relative sea-level rise and surface elevation change, sites that previously had elevation rate deficits showed a surplus. Therefore, assessments of wetland resilience that do not include subsurface processes will likely misjudge vulnerability to sea-level rise.

Downed woody debris is an important component of the forest floor, responsible for long-term storage of nutrients in many ecosystems. In large floodplain forests of the southeastern United States, wood loads are relatively low, suggesting these landscapes may promote rapid decomposition and nutrient turnover rates. The goals of this study were to identify the major factors responsible for wood decomposition and associated nutrient dynamics in an old-growth floodplain forest at Congaree National Park, South Carolina (USA). In situ decomposition and nutrient dynamics were quantified for 700 days (April 2011–March 2013) using wood from a common overstory species, red maple (Acer rubrum L.). During the study period, regional drought conditions allowed red imported fire ant (RIFA, Solenopsis invicta Buren) colonization and infestation of the study wood after 183 days in the field. Carbon (C) content decreased throughout the study, indicating net mineralization was occurring year-round. Invertebrate activity contributed to extremely fast decomposition rates (mean k 0.650 ± 0.02 year−1) and wood turnover times (mean 4.79 ± 0.15 years). In contrast to C, wood nitrogen (N) and phosphorus (P) content increased following RIFA infestation, displaying net nutrient immobilization. The rapid turnover rates measured at CONG indicate that the C storage function of woody debris may be diminished, but availability of mineralized N and P may also have increased the importance of wood stocks to support forest net primary productivity.

Salinity intrusion is responsible for changes to freshwater wetland watersheds globally, but little is known about how wetland water budgets might be influenced by small increments in salinity. We studied a forested wetland in South Carolina, USA, and installed sap flow probes on 72 trees/shrubs along a salinity gradient. Species investigated included the trees baldcypress (Taxodium distichum [L.] Rich.), water tupelo (Nyssa aquatica L.), swamp tupelo (Nyssa biflora Walt.), and the shrub waxmyrtle (Morella cerifera (L.) Small). This study improves upon past reliance on greenhouse seedling studies by adding measurements of trees/shrubs along a salinity gradient, and better describes the role of low salinity on water use in freshwater wetland forests. We measured patterns of water use related to salinity, atmospheric conditions and season, and hypothesized that salinity would influence wetland forest water use through two mechanisms: salinity disturbances would yield stands with species and size classes that transpire less and individual trees with less conductive xylem tissue (i.e., sapwood). Both hypotheses held. At salinity concentrations ranging from fresh to 3 psu, forest structural changes alone resulted in stand water use reductions from 494 mm year-1 in freshwater stands to 316 mm year-1 in stands of slightly higher salinity. Tree sapwood function (inferred from radial sap flux profiles) also changed along this gradient and reduced sap flow rates by an additional 13.3% per unit increase in salinity (psu). Thus, stand water use was further reduced to 190 mm year-1 on saline sites. We found that forest structure is not the only change that affects water use in salinized watersheds; individual tree eco-physiological responses to salinity, manifesting in different radial sap flow profiles, are important as well.

Foliar litter is an important terrestrial source of dissolved organic matter (DOM) and disinfection byproducts (DBPs) in the source water supply. Climate changes could alter precipitation patterns and hydroperiods in woody wetlands, resulting in a hydrologic shift along the low elevation gradient and change the productions of DOC and DBP precursors and their exports to source water. Here, we conducted an 80-week field decomposition study using fresh-fallen leaves along an elevation gradient, representing well-drained, relatively moist, and inundated environments, in Congaree National Park, South Carolina. The dissolved organic carbon (DOC) yield and formation potential (FP) of trihalomethanes (THMs; a dominant category of studied DBPs) were 48.9–79.7 mg-DOC/g-litter and 2.23–6.57 mg/g-litter in the freshly fallen leaf litter, respectively. The level of leachable DOM and its DBP FP decreased with time, and during the first 16 weeks of decomposition, the decomposing litter served as an important source of leachable DOM and DBP precursors. Week 28 was a turning point for DOM optical properties, with fewer tyrosine/tryptophan/soluble microbial byproduct-like compounds and more aromatic, humified, and fulvic/humic acid-like compounds. Litterfall primarily occurred from September to January, while less precipitation occurred from October to January, indicating that large amounts of DOC and DBP precursors could be leached from litterfall in February. In the first 16 weeks of field exposure study, we observed higher residual mass and lower water-extractable DOC and DTN in more inundated environments, demonstrating that the shifts of DOM composition and DBP precursors if climate reduces rainfall in the southeastern US.

Bottomland hardwood ecosystems (BLH) occupy the floodplains of low-gradient streams and rivers in the Atlantic and Gulf Coastal Plain Provinces of the southeastern United States (figure 2-1; King et al. 2012). Although they make up a relatively small portion of the landscape compared to their upland counterparts, healthy BLH forests, particularly ones with seasonal flooding and flow, have high productivity (Conner and Day 1976; Megonigal et al. 1997). They provide necessary habitat for wildlife and numerous other species and are thus critical for supporting biodiversity in the region (Sharitz and Mitsch 1993; Conner and Sharitz 2005). These forests are also responsible for the provisioning of many ecosystem goods and services, including flood control, maintenance of water quality, recreation, and nursery habitat for commercial fisheries, to name a few (King et al. 2009).

An assimilation wetland is a natural (non-constructed) wetland into which secondarily-treated, disinfected, non-toxic municipal effluent is discharged. In the Mississippi River Delta, the wetland is typically either a freshwater forested wetland (e.g., baldcypress-water tupelo) or a freshwater emergent wetland. These wetlands have been hydrology altered, some extensively, with freshwater input reduced from historical norms. Discharge of freshwater effluent with nutrients and suspended sediments into an assimilation wetland increases vegetation productivity and accretion and combats subsidence. Effluent discharge rate into an assimilation wetland depends on wetland size and effluent nutrient concentrations. Design and construction of an assimilation wetland requires a Louisiana Department of Natural Resources (LDNR) Coastal Use Permit (CUP), a Louisiana Department of Environmental Quality (LDEQ) Louisiana Pollutant Discharge Elimination System (LPDES) permit, a US Army Corps of Engineers (USACE) 404 permit, and an LDEQ Water Quality Certification, along with potential levee board permit applications. Both a feasibility study and an ecological baseline study are conducted before discharge of treated effluent begins. Assimilation wetlands are designed with a minimum of four monitoring sites; three located along a transect from the discharge to the area where surface water leaves the wetland, and the fourth, a reference area, located in an ecologically similar wetland nearby. As part of the LDEQ LPDES permit, study sites within an assimilation wetland are monitored continually for the life of the project, including vegetation productivity and species composition, sediment accretion, hydrology, and surface water nutrient and metals concentrations. There are ten active assimilation wetlands in coastal Louisiana and another four with permit applications pending. Results of annual monitoring show nutrient concentrations of surface waters decrease with distance, reaching background levels before water leaves the wetland. While nutrient concentrations decrease, vegetative productivity is enhanced. In degraded forested wetlands being used as assimilation wetlands, baldcypress and water tupelo seedlings are often planted, which thrive in the nutrient rich environment. However, nutria are attracted to vegetation with increased nutrient concentrations, and herbivory severely damaged one emergent wetland receiving municipal effluent, killing both herbaceous vegetation and unprotected tree seedlings. After culling of nutria, the wetland recovered. This introduced species must be monitored and controlled in any assimilation wetland. Here we review the history of assimilation wetlands in the Mississippi River Delta to show how advances in scientific understanding, growing regulatory sophistication, and controversy have shaped this program.

Tidal freshwater wetlands are one of the most vulnerable ecosystems to climate change and rising sea levels. However salinification within these systems is poorly understood, therefore, productivity (litterfall, woody biomass, and fine roots) were investigated on three forested tidal wetlands [(1) freshwater, (2) moderately saline, and (3) heavily salt-impacted] and a marsh along the Waccamaw and Turkey Creek in South Carolina. Mean aboveground (litterfall and woody biomass) production on the freshwater, moderately saline, heavily salt-impacted, and marsh, respectively, was 1,061, 492, 79, and 0 g m−2 year−1 versus belowground (fine roots) 860, 490, 620, and 2,128 g m−2 year−1. Litterfall and woody biomass displayed an inverse relationship with salinity. Shifts in productivity across saline sites is of concern because sea level is predicted to continue rising. Results from the research reported in this paper provide baseline data upon which coupled hydrologic/wetland models can be created to qua...

Alterations to Louisiana’s coastal landscape during the last 200 years have resulted in major changes to the natural hydrologic regime. Hurricane and tornado winds further impact coastal forests by exacerbating conditions that affect community structure, growth, and regeneration. Twenty-three years of study of forested wetlands in the Lake Verret watershed provide an opportunity to relate changes in forest communities to precipitation, drought, storms, and rising water levels. The area had two communities in 1986, but four communities were present by 2009. A major change in community composition occurred in drier sites after Hurricane Andrew (1992), when Chinese tallow (Triadica sebifera) became established. Swamp plots were generally not affected by hurricanes, but composition continued to change as a result of flooding. After Hurricane Andrew, net primary productivity (NPP) declined dramatically until 2001. Subsequent hurricanes in 2004 and 2005 also caused reductions in NPP, but recovery was rapid. Low-lying and transition areas are trending from bottomland communities toward more of a deepwater swamp as baldcypress (Taxodium distichum) and water tupelo (Nyssa aquatica) increase their stature, likely due to the system-wide flooding. Natural ridges are flooded less often and have remained primarily bottomland, though Chinese tallow (Triadica sebiferum) numbers increased following Hurricane Andrew.

Microbial fuel cell (MFC) technology has shown great potential for harvesting energy from waste organic materials. Here, we explored the potential of MFC-based electricity generation from forest detritus, a large untapped biomass pool. Electricity generation from in situ MFCs and relevant environmental parameters (i.e., carbon sources and concentrations, temperature, water depth) in a seasonally flooded freshwater cypress-tupelo wetland were monitored intensively for two flooding periods. Current outputs ranged from 0 to 1.27 mA (mean of 0.40 mA for flooding period) and were highly sensitive to environmental changes, showing seasonal and diel dependences. Excluding the influence of heavy storms, drought, or wetland icing, current output was highly temperature-dependent dielly. Seasonally, current output gradually increased in the first 3–4 months (limited by temperature) and decreased slightly during the last 1–2 months (probably limited by carbon and nutrients) of both flooding periods. Litter extract of baldcypress (Taxodium distictum) with lower C/N ratio and aromatic content showed greater stable current outputs (0.57 mA) based on 50 mg l � 1 biological oxygen demand compared to extracts of water tupelo (Nyssa aquatica) and longleaf pine (Pinus palustris), suggesting that the current output of in situ MFCs could depend on the vegetation within a wetland. Our study highlights the potential application of MFC in generating green and sustainable electricity from forest biomass for powering remote sensors in wetland ecosystems.

Forests comprise approximately 37% of the terrestrial land surface and influence global water cycling. However, very little attention has been directed towards understanding environmental impacts on stand water use (S) or in identifying rates of S from specific forested wetlands. Here, we use sapflow techniques to address two separate but linked objectives: (1) determine S in four, hydrologically distinctive South Carolina (USA) wetland forests from 2009–2010 and (2) describe potential error, uncertainty and stand-level variation associated with these assessments. Sapflow measurements were made from a number of tree species for approximately 2–8 months over 2 years to initiate the model, which was applied to canopy trees (DBH > 10–20 cm). We determined that S in three healthy forested wetlands varied from 1.97–3.97 mm day−1 or 355–687 mm year−1 when scaled. In contrast, saltwater intrusion impacted individual tree physiology and size class distributions on a fourth site, which decreased S to 0.61–1.13 mm day−1 or 110–196 mm year−1. The primary sources of error in estimations using sapflow probes would relate to calibration of probes and standardization relative to no flow periods and accounting for accurate sapflow attenuation with radial depth into the sapwood by species and site. Such inherent variation in water use among wetland forest stands makes small differences in S (

Little is known of the ability of baldcypress (Taxodium distichum (L.) L.C. Rich.) in a suppressed sub-canopy position to respond to increases in light. We measured 3 years of diameter growth response of long-suppressed baldcypress saplings to canopy gap creation in a hydrologically altered, water tupelo-dominated backswamp of the lower Roanoke River floodplain in eastern North Carolina. Baldcypress saplings responded strongly to gap creation. Saplings showed a significant positive growth trend, with mean basal area growth in gaps expanding from 2.5 cm2 in year one, 4 times that of control saplings, to 8.5 cm2 in year three, 20 times that of control saplings. Within canopy gaps, a positive trend between growth and initial sapling diameter was evident, while such a trend among controls was non-existent. Mortality was similar between gap and control saplings after 1 year, but by year three gap sapling mortality had declined to 2 %, while control mortality increased to 16 %. It is clear that long-suppressed baldcypress, sometimes at the brink of death, are able to respond vigorously to an increase in light from canopy gaps with decreased mortality and increased growth. This has important implications for wetlands requiring restoration or baldcypress management within the framework of two-age or uneven-aged silvicultural systems.

Questions What are the general tree communities found in tidal freshwater swamps along four large coastal rivers in the southeastern United States (US)? How do these communities compare to other tidal freshwater swamps in the US and South America? Locations Tidal floodplains of major rivers along the Atlantic and Gulf coasts of the southeastern US: Savannah, Altamaha, Suwannee and Apalachicola Rivers. Methods An extensive survey of trees and shrubs was conducted to describe the communities from a range of tidal freshwater swamps. River basins studied include micro-tidal (Gulf coast) and meso-tidal (Atlantic coast) regimes, and study areas were located both near and distant to primary channels. A total of 128 plots (100 m2 each) were inventoried, distributed evenly over the Savannah and Altamaha Rivers along the Atlantic coast, and the Suwannee and Apalachicola Rivers along the Gulf coast. Multivariate statistics helped discern communities and the significant indicator species in each. Results Four general communities were characterized and named according to the strongest individual indicator species in each: Water Tupelo (Nyssa aquatica) Community, Swamp Tupelo (Nyssa biflora) Community, Dwarf Palmetto (Sabal minor) Community and Cabbage Palm (Sabal palmetto) Community. Conclusions Descriptions of most tidal freshwater swamps in the southeastern US fit within the communities described in this study. Because studies that make inferences between environmental drivers (e.g. salinity, hydroperiod, hurricanes) and specific community types are best applied to the same communities (but perhaps different river systems), this work provides a framework by which tidal freshwater forested wetlands can be accurately compared based on their tree communities. We suggest that, within the broad range of our inventories, the four communities described identify the primary associations that should be tracked within most tidal freshwater swamps of the US. However, we identify some river basins in the US that do not fit this construct. Diversity of major tree communities in tidal freshwater swamps outside the US is generally much lower (with the notable exception of Amazonian hardwood tidal varzea), as are basal area values.

Hummock and hollow microtopography is pervasive in tidal freshwater swamps. Many tree species grow atop hummocks significantly more than in hollows, leading to the hypothesis that hummocks provide preferred locations for maximizing physiological proficiency of inhabiting trees that experience repeated flooding. We used thermal dissipation probes to measure the ecophysiological proficiency of a very flood-tolerant tree, Taxodium distichum, as manifested through in-situ changes in sapflow (a proxy for transpiration) in 11 trees on hummocks and 11 trees in hollows. Overall, sapflow increased significantly by 3.3 g H2O m−2 s−1 (11 %) in trees on both hummocks and hollows during flooding, contrary to our expectations. We found no significant differences in sapflow rates between T. distichum trees positioned on hummocks versus hollows in relation to discrete flood events. Coincidentally, hummock elevations were equivalent to the flood depths that promoted greatest physiological proficiency in T. distichum, suggesting a physiological role for the maintenance of hummock height in tidal swamps. While we reject our original hypotheses that flooding and positioning in hollows will reduce sapflow in T. distichum, this research reveals a potentially important feedback between hummock height, flood depth, and maximum tree physiological response.

Coastal wetlands significantly contribute to global carbon storage potential. Sea-level rise and other climate-change-induced disturbances threaten coastal wetland sustainability and carbon storage capacity. It is critical that we understand the mechanisms controlling wetland carbon loss so that we can predict and manage these resources in anticipation of climate change. However, our current understanding of the mechanisms that control soil organic matter decomposition, in particular the impacts of elevated salinity, are limited, and literature reports are contradictory. In an attempt to improve our understanding of these complex processes, we measured root and rhizome decomposition and developed a causal model to identify and quantify the mechanisms that influence soil organic matter decomposition in coastal wetlands that are impacted by sea-level rise. We identified three causal pathways: (1) a direct pathway representing the effects of flooding on soil moisture, (2) a direct pathway representing the effects of salinity on decomposer microbial communities and soil biogeochemistry, and (3) an indirect pathway representing the effects of salinity on litter quality through changes in plant community composition over time. We used this model to test the effects of alternate scenarios on the response of tidal freshwater forested wetlands and oligohaline marshes to short- and long-term climate-induced disturbances of flooding and salinity. In tidal freshwater forested wetlands, the model predicted less decomposition in response to drought, hurricane salinity pulsing, and long-term sea-level rise. In contrast, in the oligohaline marsh, the model predicted no change in response to drought and sea-level rise, and increased decomposition following a hurricane salinity pulse. Our results show that it is critical to consider the temporal scale of disturbance and the magnitude of exposure when assessing the effects of salinity intrusion on carbon mineralization in coastal wetlands. Here, we identify three causal mechanisms that can reconcile disparities between long-term and short-term salinity impacts on organic matter decomposition.

Tidal freshwater wetlands are sensitive to sea level rise and increased salinity, although little information is known about the impact of salinification on nutrient biogeochemistry in tidal freshwater forested wetlands. We quantified soil nitrogen (N) and phosphorus (P) mineralization using seasonal in situ incubations of modified resin cores along spatial gradients of chronic salinification (from continuously freshwater tidal forest to salt impacted tidal forest to oligohaline marsh) and in hummocks and hollows of the continuously freshwater tidal forest along the blackwater Waccamaw River and alluvial Savannah River. Salinification increased rates of net N and P mineralization fluxes and turnover in tidal freshwater forested wetland soils, most likely through tree stress and senescence (for N) and conversion to oligohaline marsh (for P). Stimulation of N and P mineralization by chronic salinification was apparently unrelated to inputs of sulfate (for N and P) or direct effects of increased soil conductivity (for N). In addition, the tidal wetland soils of the alluvial river mineralized more P relative to N than the blackwater river. Finally, hummocks had much greater nitrification fluxes than hollows at the continuously freshwater tidal forested wetland sites. These findings add to knowledge of the responses of tidal freshwater ecosystems to sea level rise and salinification that is necessary to predict the consequences of state changes in coastal ecosystem structure and function due to global change, including potential impacts on estuarine eutrophication.

Zoonotic diseases can be transmitted via an arthropod vector, and disease risk maps are often created based on underlying associative factors within the surrounding landscape of known occurrences. A limitation however is the ability to map disease risk at a meaningful geographic scale, and traditional regression modeling approaches may not always be appropriate. Our objective was to determine if nonlinear modeling could improve explanatory power in describing the occurrence of 2 tick-borne diseases (Lyme disease (LD) and Rocky Mountain spotted fever (RMSF)) known to occur in Tennessee. Medically diagnosed cases of LD (ICD-9: 088.81) and RMSF (ICD-9: 082.0) were extracted from a managed care organization data warehouse for the 2000–2009 time period. Four separate modeling techniques were constructed (logistic regression, classification and regression tree (CART), gradient boosted tree (GBT), and neural network (NNET)) and compared for accuracy. Results suggest that areas higher in disease prevalence were not necessarily the same areas having high predicted disease risk. GBT best explained LD occurrence (misclassification rate: 0.232; ROC: 0.789). RMSF prevalence was best explained with an NNET algorithm (misclassification rate: 0.288; ROC: 0.696). Covariates explaining disease risk included forested wetlands, urbanization, and median income. Nonlinear modeling may provide better results than traditional regression-based approaches.

Considered separately, notifiable disease registries and medical claims data have certain advantages (e.g., consistent case definitions and electronic records, respectively) and limitations (e.g., incomplete reporting and coding errors, respectively) within disease outbreak research. Combined however, these data could provide a more complete source of information. Using a retrospective space-time permutation scan statistic, zoonotic case information from a state registry system (TDH) was compared with administrative medical claims information from a managed care organization (MCO) to examine how data sources differ. Study observations included case information for four tick-borne (Lyme disease, ehrlichiosis, Rocky Mountain spotted fever, tularemia) and two mosquito-borne diseases (West Nile virus, La Crosse viral encephalitis) occurring in Tennessee. One hundred and three clusters were detected, of which nine were significant (P

Seasonally flooded, freshwater cypress-tupelo wetlands, dominated by baldcypress (Taxodium distictum), water tupelo (Nyssa aquatica), and swamp tupelo (Nyssa sylvatica var. biflora) are commonly found in coastal regions of the southeastern United States. These wetlands are threatened due to climate change, sea level rise, and coastal urban development. Understanding the natural biogeochemical cycles of nutrients in these forested wetlands as ecosystems services such as carbon sequestration and nitrogen processing can provide important benchmarks to guide conservation plans and restoration goals. In this study, surface water and soil pore water samples were collected weekly from a cypress-tupelo wetland near Winyah Bay, South Carolina and analyzed for dissolved organic carbon (DOC), dissolved organic nitrogen (DON), inorganic nitrogen, and phosphate during its flooding period between October 2010 and May 2011. DOC was further characterized by specific ultra-violet absorbance at 254 nm, spectral slope ratio (SR) (ratio of two spectral slopes between 275–295 nm and 350–400 nm), E2/E3 ratio (ratio between A254 and A365), and fluorescence excitation-emission matrix. In addition, litterfall was collected on a monthly basis for a year while the biomass of the detritus layer (i.e., decomposed duff lying on the wetland floor) was determined before and after the flooding period. Results of the field study showed that concentrations of DOC, DON, NH4 +–N, and (NO2 − + NO3 −)–N in the surface water were generally higher during the fall, or peak litterfall season (October to December), than in the spring season (March to May). Highest concentrations of 54.8, 1.48, 0.270, and 0.0205 mg L−1, for DOC, DON, NH4 +–N, and (NO2 − + NO3 −)–N respectively, in surface waters were recorded during October. Lower SUVA, but higher SR and E2/E3 ratios of DOC, were observed at the end of the flooding season comparing to the initial flooding, suggesting the wetland system converts high aromatic and large DOC molecules into smaller and hydrophilic fractions possibly through photochemical oxidation. A similar trend was observed in soil pore water, but the pore water generally had greater and relatively stable concentrations of dissolved nutrients than surface water. No obvious temporal trend in phosphate concentration and total nitrogen to total phosphorus ratio (N:P) were found. Results of the laboratory extraction and mass balance calculation suggested fresh litter was a major source of DOC whereas decomposed duff was the source of dissolved nitrogen in surface water. In summary, the biogeochemistry of this isolated cypress-tupelo wetland is not only driven by the vegetation within the wetland system but also by hydrology and weather conditions such as groundwater table position, precipitation, and temperature.

Many tidally influenced freshwater forested wetlands (tidal swamps) along the south Atlantic coast of the USA are currently undergoing dieback and decline. Salinity often drives conversion of tidal swamps to marsh, especially under conditions of regional drought. During this change, alterations in nitrogen (N) uptake from dominant vegetation or timing of N recycling from the canopy during annual litter senescence may help to facilitate marsh encroachment by providing for greater bioavailable N with small increases in salinity. To monitor these changes along with shifts in stand productivity, we established sites along two tidal swamp landscape transects on the lower reaches of the Waccamaw River (South Carolina) and Savannah River (Georgia) representing freshwater (≤0.1 psu), low oligohaline (1.1–1.6 psu), and high oligohaline (2.6–4.1 psu) stands; the latter stands have active marsh encroachment. Aboveground tree productivity was monitored on all sites through monthly litterfall collection and dendrometer band measurements from 2005 to 2009. Litterfall samples were pooled by season and analyzed for total N and carbon (C). On average between the two rivers, freshwater, low oligohaline, and high oligohaline tidal swamps returned 8,126, 3,831, and 1,471 mg N m−2 year−1, respectively, to the forest floor through litterfall, with differences related to total litterfall volume rather than foliar N concentrations. High oligohaline sites were most inconsistent in patterns of foliar N concentrations and N loading from the canopy. Leaf N content generally decreased and foliar C/N generally increased with salinization (excepting one site), with all sites being fairly inefficient in resorbing N from leaves prior to senescence. Stands with higher salinity also had greater flood frequency and duration, lower basal area increments, lower tree densities, higher numbers of dead or dying trees, and much reduced leaf litter fall (103 vs. 624 g m−2 year−1) over the five study years. Our data suggest that alternative processes, such as the rate of decomposition and potential for N mineralization, on tidal swamp sites undergoing salinity-induced state change may be more important for controlling N biogeochemical cycling in soils than differences among sites in N loading via litterfall.

Aims In recent years, there has been an increased interest in examining changes in forest systems in response to drought, flooding, hurricanes and climate change. In the southern United States, forested wetlands are of special interest because of the extent of these forests. Coastal plain forested wetlands are among the most vulnerable to these climatic impacts. One of the problems in developing management practices for these coastal areas is the difficulty in adequately describing productivity relations and predicting how the structure and function of these communities might be affected by natural or anthropogenic disturbances. Community response to environmental change often occurs over a period of years, and the majority of reported studies are for 1–3 years in duration. This study documents long-term changes (10 years) in structure, composition and growth along a catena of high water table forested sites of an ancient beach ridge landscape in coastal South Carolina. Methods Aboveground net primary production (ANPP) of trees was monitored from 2000 to 2009 on three sites within a longleaf pine-swamp blackgum forest system on the southern end of the Waccamaw Neck area of Georgetown County, SC. Permanent study plots (20 3 25 m) were established across a moisture gradient (Dry, Intermediate, and Wet). Water levels were continuously monitored, litterfall was measured monthly and growth of trees >10 cm diameter at breast height was monitored on an annual basis. Annual litterfall and tree production values were summed to provide estimates of ANPP. Important Findings The study site was under severe drought conditions July 2001 through late summer 2002 and again in 2007. Diameter growth was affected in all three sites, but with different patterns. It seems that diameter growth in the Wet site was more sensitive to drought conditions in 2001–02 and 2007 than either Dry or Intermediate sites. While droughts did not seem to have a significant impact on litterfall in the Wet site, litterfall in the Intermediate site was more sensitive to the drought than either Dry or Wet sites. ANPP was significantly lower in both Intermediate and Wet sites in 2001 at 1 000 g/m) occurred in the Intermediate and Wet sites in 2003 following a return to more normal water levels at the end of the drought. Maximum tree production occurred on the Wet site in 2003 (657 g/m), which exceeded total ANPP of any site in 2001. In the Dry site, ANPP remained relatively consistent throughout the study when compared to Wet and Intermediate sites. While litterfall estimates are well defined with 3–5 years of data, data collection is continuing to assess impact of drought on stem growth across the gradient, which is still not clear with 10 years of data.

Tidal freshwater forests in coastal regions of the southeastern United States are undergoing dieback and retreat from increasing tidal inundation and saltwater intrusion attributed to climate variability and sea-level rise. In many areas, tidal saltwater forests (mangroves) contrastingly are expanding landward in subtropical coastal reaches succeeding freshwater marsh and forest zones. Hydrological characteristics of these low-relief coastal forests in intertidal settings are dictated by the influence of tidal and freshwater forcing. In this paper, we describe the application of the Sea Level Over Proportional Elevation (SLOPE) model to predict coastal forest retreat and migration from projected sea-level rise based on a proxy relationship of saltmarsh/mangrove area and tidal range. The SLOPE model assumes that the sum area of saltmarsh/mangrove habitat along any given coastal reach is determined by the slope of the landform and vertical tide forcing. Model results indicated that saltmarsh and mangrove migration from sea-level rise will vary by county and watershed but greater in western Gulf States than in the eastern Gulf States where millions of hectares of coastal forest will be displaced over the next century with a near meter rise in relative sea level alone. Substantial losses of coastal forests will also occur in the eastern Gulf but mangrove forests in subtropical zones of Florida are expected to replace retreating freshwater forest and affect regional biodiversity. Accelerated global eustacy from climate change will compound the degree of predicted retreat and migration of coastal forests with expected implications for ecosystem management of State and Federal lands in the absence of adaptive coastal management.

Tidal freshwater forested wetlands occupy a relatively narrow range, occurring where wind and lunar tides interact with coastal river systems, causing freshwater flooding onto the floodplain. A prominent component of this wetland type is hummock and hollow microtopography. Tidal freshwater forested wetlands along the Savannah River were differentiated into backswamp and streamside areas, and the degree to which trees occupied hummocks and hollows were compared at three scales: landscape (backswamp vs. streamside), tree community, and species (within community). The community- and species-level analyses were extended to determine whether trees were using either hummocks or hollows in a nonrandom manner. Trees in the backswamp setting were found to use hummocks more than trees in the streamside setting. At the community scale, three of the five treatment groups investigated differed based on the degree to which trees on hummocks outnumber trees in hollows. Further examination of microtopography usage confirmed that hummocks are used significantly more than hollows in two communities, both of which are located in the backswamp setting. Though no tree community used hollows significantly more than hummocks, species-level analyses confirmed that, within a specific tree community in the streamside setting, alder (Alnus serrulata [Ait.] Willd.) is found more in hollows than on hummocks. Fourteen different species between the two communities in the backswamp setting are found on hummocks more than in hollows, and only one species, sweetgum (Liquidambar styraciflua L.), is found more on hummocks than in hollows in the tree community in the streamside setting.

This report documents changes in forest structure and growth potential of dominant trees in salt-impacted tidal and non-tidal baldcypress wetlands of the southeastern United States. We inventoried basal area and tree height, and monitored incremental growth (in basal area) of codominant baldcypress (Taxodium distichum) trees monthly, for over four years, to examine the inter-relationships among growth, site fertility, and soil physico-chemical characteristics. We found that salinity, soil total nitrogen (TN), flood duration, and flood frequency affected forest structure and growth the greatest. While mean annual site salinity ranged from 0.1 to 3.4 ppt, sites with salinity concentrations of 1.3 ppt or greater supported a basal area of less than 40 m2/ha. Where salinity was < 0.7 ppt, basal area was as high as 87 m2/ha. Stand height was also negatively affected by higher salinity. However, salinity related only to soil TN concentrations or to the relative balance between soil TN and total phosphorus (TP), which reached a maximum concentration between 1.2 and 2.0 ppt salinity. As estuarine influence shifts inland with sea-level rise, forest growth may become more strongly linked to salinity, not only due to salt effects but also as a consequence of site nitrogen imbalance.

Little is known about the effectiveness of mangroves in suppressing water level heights during landfall of tropical storms and hurricanes. Recent hurricane strikes along the Gulf Coast of the United States have impacted wetland integrity in some areas and hastened the need to understand how and to what degree coastal forested wetlands confer protection by reducing the height of peak water level. In recent years, U.S. Geological Survey Gulf Coast research projects in Florida have instrumented mangrove sites with continuous water level recorders. Our ad hoc network of water level recorders documented the rise, peak, and fall of water levels (± 0.5 hr) from two hurricane events in 2004 and 2005. Reduction of peak water level heights from relatively in-line gages associated with one storm surge event indicated that mangrove wetlands can reduce water level height by as much as 9.4 cm/km inland over intact, relatively unchannelized expanses. During the other event, reductions were slightly less for mangroves along a river corridor. Estimates of water level attenuation were within the range reported in the literature but erred on the conservative side. These synoptic data from single storm events indicate that intact mangroves may support a protective role in reducing maximum water level height associated with surge.

Above-ground net primary productivity (NPP), decomposition of water tupelo leaves, and nutrient dynamics were investigated from 2000 to 2002 within a coastal, tidally influenced forested wetland on Bull Island, SC. Flooding of the island is influenced by flooding events of the Pee Dee River (PD) on the western side of the island, Big and Little Bull Creeks (BC) on the eastern side, and daily tidal fluctuations. Above-ground NPP ranged between 477 g/m2 and 1117 g/m2 while mean above-ground NPP was 743 g/m2 when all data were combined regardless of growing seasons or sites. Mean annual litterfall and stemwood production were 463 g/(m2 year) and 279 g/(m2 year), respectively, for all three growing seasons. Analyses also showed that the 3-year average above-ground NPP was significantly higher (p

The frequency of hurricane landfall in a given coastal stretch may play a more important role in the ecology of coastal forests than previously thought because of direct and indirect impacts of fallen trees and the introduction of salt water that lingers long after the storm passes. Findings show that surge events can inundate interior freshwater forests many miles from the coast and elevate soil salinities twofold to threefold. These elevated salinities may contribute to delayed mortality of certain tree species and set the stage for eventual forest decline and dieback.