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The fast reactor is gradually being paid attention to in nuclear power due to a better breeding effect, and its economy is also directly followed. Due to the restriction of the core power density, the core power level is directly related to the volume of the core active area. When the power level and the volume of the core gradually increase, the mass of uranium and 235U in the core at the beginning of life will gradually increase on the basis of a critical uranium inventory. At the same time, due to the gradual increase in the breeding effect, the utilization rate of uranium resources at the end of life will gradually increase. Due to the characteristics of the large critical uranium mass and high breeding effect, the mass of uranium and 235U in the low power level core at the beginning of life cannot be further reduced because of the critical uranium inventory. The lifetime of the high power level core should be extended to use the wealthy reactivity of the core because of the improvement in the breeding effect. However, due to the requirements of the nuclear power refuel time and the limitation to fuel consumption, the breeding effect brought by the improvement in the core power level and the size of the active zone may be less effective. In this article, the power level and economy of lead-based modular nuclear power are analyzed, and the economy of different power level fast reactors with 2,000 EFPD lifetime such as 100 MWt, 300 MWt, 500 MWt, 700 MWt, and 1000 MWt thermal power is designed and analyzed. By analyzing the invention of uranium and 235U and the fuel utilization of different core schemes, the best economic analysis of the single reactor power of the current lead-based modular reactor is proposed and the foundation of the lead-based modular nuclear power is provided.

Previous studies have shown that ferroptosis of vascular smooth muscle cells (VSMCs) is involved in the development of aortic dissection (AD) and that histone methylation regulates this process. SP2509 acts as a specific inhibitor of lysine-specific demethylase 1 (LSD1), which governs a variety of biological processes. However, the effect of SP2509 on VSMC ferroptosis and AD remains to be elucidated. This aim of this study was to investigate the role and underlying mechanism of SP2509-mediated histone methylation on VSMC ferroptosis. Here, a mouse model of AD was established, and significantly reduced levels of H3K4me1 and H3K4me2 (target of SP2509) were found in the aortas of AD mice. In VSMCs, SP2509 treatment led to a dose-dependent increase in H3K4me2 levels. Furthermore, we found that SP2509 provided equivalent protection to ferrostatin-1 against VSMC ferroptosis, as evidenced by increased cell viability, decreased cell death and lipid peroxidation. RNA-sequencing analysis and subsequent experiments revealed that SP2509 counteracted cystine deficiency-induced response to inflammation and oxidative stress. More importantly, we demonstrated that SP2509 inhibited the expression of TFR and ferritin to reduce intracellular iron levels, thereby effectively blocking the process of ferroptosis. Therefore, our findings indicate that SP2509 protects VSMCs from multiple stimulus-induced ferroptosis by reducing intracellular iron levels, thereby preventing lipid peroxidation and cell death. These findings suggest that SP2509 may be a promising drug to alleviate AD by reducing iron deposition and VSMC ferroptosis. [Display omitted] • H3K4me1 and H3K4me2 levels were significantly reduced in mouse aortic dissection tissues. • SP2509 could inhibit CD- and IKE-induced ferroptosis by reducing inflammation and oxidative stress. • SP2509 could alleviate intracellular Fe2+ accumulation, thus preventing oxidative stress and ferroptosis. [ABSTRACT FROM AUTHOR]

AbstractThe combined cycle engine based on turbine is typical in the field of cycle engines. However, the problem of high temperature heat load of turbine blades is serious. In order to solve this kind of problem, the method of using hydrocarbon fuel as cooling medium to improve the cooling efficiency of turbine blades are proposed. In this paper, the flow and heat transfer characteristics of RP-3 aviation kerosene in a rotating U-shaped channel under supercritical pressure were studied by numerical simulation. The flow and heat transfer characteristics of RP-3 aviation kerosene under supercritical pressure at different rotational speeds were numerically simulated by SST k-ω model. The results show that the centrifugal section and the horizontal section show obvious fluid stratification, and the mainstream distribution in the centripetal section is relatively uniform due to the bending effect and rotational buoyancy. Compared with the heat transfer capacity in the static state of the centrifugal section, the heat transfer of the fluid in the rotating state deteriorates, recovers and strengthens with the increase in the rotational speed. Moreover, compared with the centripetal section, the rotational speed has a particularly significant effect on the heat transfer performance in the centrifugal section and the horizontal section. When the rotational speed exceeds 300 rpm, the deterioration trend of heat transfer is weakened and the overall heat transfer performance is greatly improved. [ABSTRACT FROM AUTHOR]

Background and purpose: Despite the growing awareness of academic fraud, its prevalence in the field of neurology has not been fully assessed. This review aims to analyze the characteristics of the retracted papers in the field of neurology and the reasons for the retraction to better understand the trends in this area and to assist to avoid retraction incidents. Methods: A total of 79 papers were included, which pertained to 22 countries and 64 journals. The marking methods for retracting original papers included watermarks (89.04%), retracted signs in the text (5.48%) and no prompt (5.48%). The median M (interquartile range [IQR]) of citations in retractions in neurology was 7 (41). Studies continued to be cited after retraction with an M (IQR) of 3 (16). The journal impact factor was between 0 and 157.335, with an M (IQR) of 5.127 (3.668). 45.21% and 31.51% papers were mainly published in the first and second quartile journals, respectively. The M (IQR) time elapsed between publication and retraction was 32 (44) months. The reasons for retraction included two major categories, academic misconduct (79.75%) and academic unintentional mistakes (20.25%). Results and conclusions: The number of retractions in neurology has been on the rise over the past decade, with fabricated academic misconduct being the main cause of the retractions. Due to the long time lag between publication and retraction, a number of unreliable findings continue to be cited following retraction. In addition to the requisite standards of academic ethics, augmenting research training and fostering interdisciplinary collaboration are crucial in enhancing research integrity. [ABSTRACT FROM AUTHOR]

Purpose: Bone loss has been found to occur frequently in patients with particular metabolic disorders that are likely associated with certain kidney stone composition. Thus, we compared the bone mineral density (BMD) of patients with different kidney stone compositions. Patients and methods: A total of 204 consecutive patients who exhibited stone formation with calcium oxalate (CaOx), calcium phosphate (CaP), uric acid (UA), and magnesium ammonium phosphate (MAP) underwent 24 h urine test and BMD measurement. BMD was measured by dual X-ray absorptiometry at the lumbar spine (LS) and femoral neck (FN). The Z-score was used to express BMD. A BMD Z-score ≤ − 2 was defined as a diagnostic threshold for bone loss. Results: Amongst the patients, 38 had an LS BMD Z-score of ≤ − 2, but only 2 had FN BMD Z-score of ≤ − 2. The group with an LS BMD Z-score of ≤ − 2 exhibited significantly larger male − female ratio, higher frequency of hypercalciuria and CaP, and lower frequency of MAP than the group with an LS BMD Z-score of > − 2. Reduced LS BMD was most remarkable in the CaP group, followed by the CaOx, UA, and MAP groups. The LS BMD Z-score of hypercalciuric patients was significantly lower than that of normocalciuric patients only in the CaP group. Conclusion: Patients with different kidney stone compositions presented different BMD status. Using this information may facilitate medical decision-making in patients with kidney stone who should undergone BMD earlier. [ABSTRACT FROM AUTHOR]

Tripartite motif-containing protein 21 (TRIM21), an E3 ubiquitin ligase, plays a critical role in the host antiviral response. However, the mechanism and antiviral spectrum of TRIM21 in influenza A virus (IAV) remain unclear. Here, we report that TRIM21 inhibits the replication of various IAV subtypes by targeting matrix protein 1 (M1) from H3/H5/H9, but not H1 and H7 M1. Mechanistically, TRIM21 binds to the residue R95 of M1 and facilitates K48 ubiquitination of M1 K242 for proteasome-dependent degradation, leading to the inhibition of H3, H5, and H9 IAV replication. Interestingly, the recombinant viruses with M1 R95K or K242R mutations were resistance to TRIM21 and exhibited more robust replication and severe pathogenicity. Moreover, the amino acid sequence M1 proteins, mainly from avian influenza such as H5N1, H7N9, H9N2, ranging from 1918 to 2022, reveals a gradual dominant accumulation of the TRIM21-driven R95K mutation when the virus jumps into mammals. Thus, TRIM21 in mammals' functions as a host restriction factor and drives a host adaptive mutation of influenza A virus. Author summary: IAVs use various adaptive mutation strategies to evade host restriction. In the present study, we found that TRIM21 in mammals directly bound to M1 R95 site resulted in proteasome-degradation of M1 via K48-linked ubiquitination at K242 and thereby inhibited replication of IAV, which caused inhibitory pressure for host adaptive mutation of IAVs at R95 of M1. This study highlights the association between host restriction factor and virus evolution. [ABSTRACT FROM AUTHOR]

Lithium–sulfur (Li–S) batteries are hindered by the undesired shuttle effect and sluggish electrochemical conversion kinetics. Herein, a well‐designed CoFe2O4@reduced graphene oxide (CFO@rGO) composite is used to modify the separator to develop a multifunctional polysulfide barrier. Density functional theory (DFT) calculations confirm that highly electronegative oxygen ions in CFO tend to bond with transition metal (TM) ions at octahedral (Oh) sites, which induces the formation of FeS and CoS bonds between CFO and polysulfides. This indicates that CFO can effectively anchor polysulfides. Furthermore, the low Li2S decomposition energy barrier and Li+ diffusion energy barrier reveal that CFO can accelerate the redox reaction kinetics of sulfur species. Electronic structure calculations speculate that the low‐energy barrier can be attributed to the electron‐hopping phenomenon between TM ions of different valence states at Oh sites. Benefiting from these advantages, a CFO@rGO/PP separator demonstrates satisfactory cycling performance (0.087% capacity decay rate at 2C with 500 cycles) and superb rate performance (686 mAh g−1 at 5C). This work provides a valuable reference for future research on spinel‐type materials as electrocatalysts for Li–S batteries. [ABSTRACT FROM AUTHOR]

Influenza A virus (IAV) expresses several accessory proteins to limit host anti-viral restriction factors to facilitate viral replication. The Ten-Eleven Translocation 2 (TET2) is a methylcytosine dioxygenase that promotes DNA demethylation by catalyzing the oxidation of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC), which plays a vital role in hematopoiesis and immunity. Here we report that TET2 is a host restriction factor that limits IAV replication. But IAV endoribonuclease PA-X is able to remove the replication restriction by binding to TET2 mRNA and driving TET2 mRNA degradation to reduce TET2 expression during infection. Genetic inactivation of TET2 markedly enhances IAV replication in vitro and in vivo. Mechanistically, we found that TET2 regulates demethylation and transcription of STAT1 and some interferon-stimulated genes (ISGs), including ISG15, ISG20, and IFIT5, so the loss of TET2 greatly impairs type I Interferon signaling. Furthermore, we confirmed that TET2-mediated demethylation of the STAT1 gene is critical for interferon anti-viral activity. Our study demonstrates that the host TET2 is essential to the innate immune response against IAV infection. Author summary: Influenza A virus (IAV) severely risks public health and economic stability. The endoribonuclease PA-X of IAV can limit host gene expression to facilitate viral replication. The Ten-Eleven Translocation 2 (TET2)-mediated DNA demethylation, which promotes gene transcription, is essential for host hematopoiesis and immunity. Here, we report that host TET2 mRNA can be targeted by PA-X protein for degradation, resulting in reduced TET2 expression during IAV infection. Notably, loss of TET2 markedly enhances IAV replication. Our study further confirmed that TET2 is responsible for the demethylation and expression of STAT1, ISG15, ISG20, and IFIT5 genes, which belong to type I interferon signaling and contribute to anti-viral immune response. Additionally, we identified that STAT1 is a critical factor in response to TET2 in the anti-IAV signaling. Taken together, we demonstrated that IAV inhibits TET2 expression by PA-X to reduce STAT1 and ISG expression, leading to immune escape and enhanced viral replication. Our research can provide a novel target for therapeutics to inhibit IAV infection and reduce pathology. [ABSTRACT FROM AUTHOR]

Ethylene (C2H4) is a major chemical for the modern society, and new technologies for its production are of strategic importance globally. Recently, oxidative dehydrogenation of ethane (C2H6) using CO2 as a milder oxidant (CO2‐ODH) is proposed as a potential way for C2H4 production, and development of effective catalysts for the process is drawing wide attention. Here, we report on a facilely prepared ZSM‐5 supported Zn system, i. e., ZnZSM‐5, which showed great promise in CO2‐ODH. Samples with different Zn loadings were prepared and evaluated, and the highest performance was obtained over 0.05ZnZSM‐5 at 700 °C and a CO2:C2H6 feeding ratio of 5 : 1. During 340 min TOS, the C2H6 conversion decreased moderately from 36.2 % to 23.1 %, and the C2H4 yield stabilized at 21.9 % to 27.9 %. Based on systematic characterization and investigation of reaction conditions, the structure‐performance relationship and reaction network were discussed in detail. [ABSTRACT FROM AUTHOR]

Pig meat off-flavor is attributed to trimethylamine (TMA) concentration, and it is considered as the precursor of the fishy off-flavor problems. In this study, TMA concentrations in pig meat were determined, and the interactions with breed and gender effects were discussed. In addition, the TMA threshold for meat off-flavor and pig meat natural quality was measured in relation to meat storage and movement, and the influential factors including the pig breed and storage time were discussed. The results indicated positive effects on the precursor of the fishy off-flavor and the TMA threshold. Native breeds were found to have lower TMA concentrations than European breeds (P-1, and this occurred after 35–38 h of storage. The natural qualities, such as appearance, flavor, color and overall acceptable scores declined significantly after 4 days in storage (P

Atmospheric particulate pollution poses a great danger to the environment and human health, and there is a strong need to develop equipment for collecting and separating particulate matter of different particle sizes to study the effects of particulate matter on human health. A virtual impactor is a particle separation device based on the principle of inertial separation which provides scientific guidance for identifying the composition characteristics of particles. Much existing virtual impactor research focuses on the design of structural dimensions with little exploration of the effect of fluid properties on performance. In this paper, a microfluidic chip with a cutoff diameter of 1.85 µm was designed based on computational fluid dynamics and numerically simulated via finite element analysis to analyze important parameters such as inlet flow rate, splitting ratio and fluid properties. By numerical simulation of the split ratio, we found that the obtained collection efficiency curves could not be combined into one characteristic curve by the Stk 0.5 scaling method. We therefore propose a modified Stokes number equation for predicting the cutoff diameter at different splitting ratios. The collection efficiency curves of different fluids as microfluidic chip media were plotted, and the results show that the cut particle size was reduced from 2.5 µm to 1.85 µm after replacing conventional fluid air with CO2 formed by dry ice sublimation. This is a decrease of approximately 26%, which is superior to other existing methods for reducing the cutoff diameter. [ABSTRACT FROM AUTHOR]

Ubiquitination is an important reversible post-translational modification. Many viruses hijack the host ubiquitin system to enhance self-replication. In the present study, we found that Avibirnavirus VP3 protein was ubiquitinated during infection and supported virus replication by ubiquitination. Mass spectrometry and mutation analysis showed that VP3 was ubiquitinated at residues K73, K135, K158, K193, and K219. Virus rescue showed that ubiquitination at sites K73, K193, and K219 on VP3 could enhance the replication abilities of infectious bursal disease virus (IBDV), and that K135 was essential for virus survival. Binding of the zinc finger domain of TRAF6 (TNF receptor associated factor 6) to VP3 mediated K11- and K33-linked ubiquitination of VP3, which promoted its nuclear accumulation to facilitate virus replication. Additionally, VP3 could inhibit TRAF6-mediated NFKB/NF-κB (nuclear factor kappa B) activation and IFNB/IFN-β (interferon beta) production to evade host innate immunity by inducing TRAF6 autophagic degradation in an SQSTM1/p62 (sequestosome 1)-dependent manner. Our findings demonstrated a macroautophagic/autophagic mechanism by which Avibirnavirus protein VP3 blocked NFKB-mediated IFNB production by targeting TRAF6 during virus infection, and provided a potential drug target for virus infection control. Abbreviations: ATG: autophagy related; BafA1: bafilomycin A1; CALCOCO2/NDP52: calcium binding and coiled-coil domain 2; Cas9: CRISPR-associated protein 9; CHX: cycloheximide; Co-IP: co-immunoprecipitation; CRISPR: clustered regularly interspaced short palindromic repeats; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GST: glutathione S-transferase; IBDV: infectious bursal disease virus; IF: indirect immunofluorescence; IFNB/IFN-β: interferon beta; mAb: monoclonal antibody; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MOI: multiplicity of infection; MS: mass spectrometry; NFKB/NF-κB: nuclear factor kappa B; NBR1: NBR1 autophagy cargo receptor; OPTN: optineurin; pAb: polyclonal antibody; PRRs: pattern recognition receptors; RNF125: ring finger protein 125; RNF135/Riplet: ring finger protein 135; SQSTM1/p62: sequestosome 1; TAX1BP1: tax1 binding protein1; TCID50: 50% tissue culture infective dose; TRAF3: TNF receptor associated factor 3; TRAF6: TNF receptor associated factor 6; TRIM25: tripartite motif containing 25; Ub: ubiquitin; Wort: wortmannin; WT: wild type. [ABSTRACT FROM AUTHOR]

Despite being a critical communication skill, grasping humor is challenging—a successful use of humor requires a mixture of both engaging content build-up and an appropriate vocal delivery (e.g., pause). Prior studies on computational humor emphasize the textual and audio features immediately next to the punchline, yet overlooking longer-term context setup. Moreover, the theories are usually too abstract for understanding each concrete humor snippet. To fill in the gap, we develop DeHumor, a visual analytical system for analyzing humorous behaviors in public speaking. To intuitively reveal the building blocks of each concrete example, DeHumor decomposes each humorous video into multimodal features and provides inline annotations of them on the video script. In particular, to better capture the build-ups, we introduce content repetition as a complement to features introduced in theories of computational humor and visualize them in a context linking graph. To help users locate the punchlines that have the desired features to learn, we summarize the content (with keywords) and humor feature statistics on an augmented time matrix. With case studies on stand-up comedy shows and TED talks, we show that DeHumor is able to highlight various building blocks of humor examples. In addition, expert interviews with communication coaches and humor researchers demonstrate the effectiveness of DeHumor for multimodal humor analysis of speech content and vocal delivery. [ABSTRACT FROM AUTHOR]

Lithium–sulfur batteries have received extensive interest owing to their exceptionally high energy density. Nonetheless, their practical implementation is still impeded by the shuttle effect of polysulfides and sluggish conversion kinetics. Considering that, a porous 2D defective zeolitic imidazolate framework‐7 (ZIF‐7) with abundant active edges is rationally designed as multifunctional sulfur carriers for Li–S batteries. The 2D ZIF‐7 enables uniform distribution of sulfur and rapid Li‐ion diffusion, while rich edges facilitate sufficient exposure to active sites capturing and catalyzing polysulfides. In addition, the nitrogen defects on edge sites can further accelerate the transformation of polysulfides and decrease the energy barrier of Li2S decomposition. Consequently, the Li–S batteries demonstrate surprisingly practical prospects with a stable capacity of 676.9 mAh g−1 over 500 cycles at 1 C (capacity retention rate = 72.3%). When assembled into a pouch cell at 2.3 mg cm−2, it still exhibits a high capacity of 901.1 mAh g−1 after 100 cycles at 0.1 C. This work offers a rational structural design strategy to tackle the challenges of the sulfur cathode. [ABSTRACT FROM AUTHOR]

The virtual impactor, as an atmospheric particle classification chip, provides scientific guidance for identifying the characteristics of particle composition. Most of the studies related to virtual impactors focus on their size structure design, and the effect of temperature in relation to the dynamic viscosity on the cut−off diameter is rarely considered. In this paper, a new method that can reduce the cut−off particle size without increasing the pressure drop is proposed. Based on COMSOL numerical simulations, a new ultra−low temperature virtual impactor with a cut−off diameter of 2.5 μm was designed. A theoretical analysis and numerical simulation of the relationship between temperature and the performance of the virtual impactor were carried out based on the relationship between temperature and dynamic viscosity. The effects of inlet flow rate (Q), major flow channel width (S), minor flow channel width (L) and split ratio (r) on the performance of the virtual impactor were analyzed. The collection efficiency curves were plotted based on the separation effect of the new virtual impactor on different particle sizes. It was found that the new ultra−low temperature approach reduced the PM2.5 cut−off diameter by 19% compared to the conventional virtual impactor, slightly better than the effect of passing in sheath gas. Meanwhile, the low temperature weakens Brownian motion of the particles, thus reducing the wall loss. In the future, this approach can be applied to nanoparticle virtual impactors to solve the problem of their large pressure drop. [ABSTRACT FROM AUTHOR]

[Display omitted] • mCMLG was designed for tumor immunostimulation combined with chemodynamic therapy. • Depletion of lactate was utilized to indirectly reprogram the TAMs from M2 to M1 phenotype. • Generation of ·OH from MnO 2 in response to H 2 O 2 could directly reprogram TAMs. • Autophagy inhibitor was released to amplify the therapeutic effect of ·OH. Reprogramming of tumor associated macrophages (TAMs) from M2 to M1 phenotype is becoming a feasible strategy for tumor immunotherapy, however, its efficacy is potentially constrained by the acellular components of immunosuppressive tumor microenvironment (TME). Herein, hollow MnO 2 (HMnO 2) nanoparticles were loaded with autophagy inhibitor chloroquine (CQ), carried both lactate oxidase (LOX) and glucose oxidase (GOX) on their surface, and further were camouflaged with tumor cell membrane, forming the final mCMLG nanoparticles for TME immunostimulation. After intravenous injection, mCMLG nanoparticles effectively accumulated in the tumor area and were taken up by tumor cells. Under the acidic conditions, MnO 2 reacted with endogenous hydrogen peroxide (H 2 O 2) to generate O 2 and Mn2+, resulting in the exposure of LOX and GOX as well as the release of CQ. The exposed enzymes reduced the level of lactate (a predominant acellular component), alleviating the inhibition on TAM reprogramming; hydroxyl radical (·OH) generated by Fenton-like reaction of Mn2+ could directly promote this reprogramming, thus effectively facilitating the reprogramming of TME from immunosuppressive to immunostimulatory. Moreover, ·OH also induced chemodynamic therapy (CDT) effect against tumor cells, which was further amplified by CQ. This study corroborates that mCMLG nanoparticles can effectively inhibit tumor growth through the enhanced immunotherapy and CDT. [ABSTRACT FROM AUTHOR]

Parameter quantization with lower bit width is the common approach to reduce the computation loads in CNN inference. With the parameters being replaced by fixed-width binaries, multiplication operations can be replaced by the look-up-table (LUT), where the multiplier-multiplicand operands serve as the table index, and the precalculated products serve as table elements. Because the histogram profiles of the parameters in different layers/channels differ significantly in CNN, previous LUT-based computation methods have to use different LUTs for each layer/channel, and consequently demand larger memory space along with extra access time and power consumption. In this work, we first normalize the parameters Gaussian profiles of different layers/channels to have similar means and variances, and further quantize the normalized parameters into fixed width through nonlinear quantization. Because of the normalized parameters profile, we can use one single compact LUT ($16\times 16$ entries) to replace all multiplication operations in the whole network. Furthermore, the normalization procedure also reduces the errors induced from quantization. Experiments demonstrate that with a compact 256-entry LUT, we can achieve the accuracy comparable to the results from 32-bit floating-point calculation; while significantly reducing the computation loads and memory spaces, along with power consumption and hardware resources. [ABSTRACT FROM AUTHOR]

The article demonstrates that the energy method utilized in computational mechanics is a special form of the variational method through mathematical deduction and comparison to the two methods. The result discloses a basic fact that mechanical computations performed by different methods are intrinsically related in some way. The article also points out that a deep study on this intrinsic relation is necessary and helpful to guarantee loyalty of the computational results to the computed objectives.

Spatiotemporal targeting of tumor-associated macrophages (TAMs) and tumor cells is emerging as a promising strategy for tumor therapy. Tumor microcalcifications that specifically bind to bisphosphonates are potentially used to design efficient relay drug delivery nanosystems to achieve spatiotemporal drug modulation. Here, we developed manganese dioxide (MnO 2)-embedded and LyP-1 peptide-labeled liposomal nanoparticles (NPs) for photodynamic immunotherapy of breast cancer; zoledronic acid (Zol) was encapsulated in the hydrophilic cavity of liposomes, and a hydrophobic photosensitizer (IR780) was embedded in the phospholipid bilayer of liposomes. These Lipo Zol/IR NPs generated O 2 bubbles through MnO 2 in response to H 2 O 2 in the tumor microenvironment, leading to the degradation of the liposomal membrane, which triggered the release of Zol and provided O 2 for photodynamic therapy. The released Zol attached to microcalcifications and was selectively phagocytosed by TAMs, leading to the induction of death or repolarization of TAMs from the immunosuppressive M2 phenotype to the immunostimulatory M1 phenotype. The remaining liposomal fragments embedded with IR780 then preferentially targeted tumor cells through LyP-1 peptide and produced abundant reactive oxygen species (ROS) under near infrared (NIR) laser irradiation, resulting in the death of tumor cells and mild immune activation. All in vitro and in vivo studies demonstrated the effective photodynamic and immunoregulatory performance of Lipo Zol/IR NPs. Spatiotemporal targeting of tumor-associated macrophages (TAMs) and tumor cells remains a challenge in tumor photodynamic immunotherapy for promoting synergy and reducing side effects. Here, we developed tumor microcalcification-mediated relay drug delivery nanoliposomes for breast cancer therapy. H 2 O 2 in the tumor microenvironment (TME) triggers the breakage of nanoliposomes, thereby causing the separation of zoledronic acid (Zol) and the photosensitizer IR780 and allowing them to perform their respective functions. Microcalcifications enable Zol to target TAMs, resulting in immunomodulation. LyP-1 guides IR780 to target tumor cells for PDT with adequate O 2 supply. These nanoliposomes enable precise spatiotemporal targeting of different types of cells in the TME and promote the synergy between immunotherapy and PDT while ensuring the effectiveness of both methods. [Display omitted] [ABSTRACT FROM AUTHOR]

Multimodal sentiment analysis aims to recognize people's attitudes from multiple communication channels such as verbal content (i.e., text), voice, and facial expressions. It has become a vibrant and important research topic in natural language processing. Much research focuses on modeling the complex intra- and inter-modal interactions between different communication channels. However, current multimodal models with strong performance are often deep-learning-based techniques and work like black boxes. It is not clear how models utilize multimodal information for sentiment predictions. Despite recent advances in techniques for enhancing the explainability of machine learning models, they often target unimodal scenarios (e.g., images, sentences), and little research has been done on explaining multimodal models. In this paper, we present an interactive visual analytics system, M2 Lens, to visualize and explain multimodal models for sentiment analysis. M2 Lens provides explanations on intra- and inter-modal interactions at the global, subset, and local levels. Specifically, it summarizes the influence of three typical interaction types (i.e., dominance, complement, and conflict) on the model predictions. Moreover, M2 Lens identifies frequent and influential multimodal features and supports the multi-faceted exploration of model behaviors from language, acoustic, and visual modalities. Through two case studies and expert interviews, we demonstrate our system can help users gain deep insights into the multimodal models for sentiment analysis. [ABSTRACT FROM AUTHOR]

Iron metabolism is involved in numerous physiological processes such as erythropoiesis, oxidative metabolism. However, the in vivo physiological functions of the iron metabolism-related gene Hfe in immune response during viral infection remain poorly understood. Here, we identified 5 iron metabolism-associated genes specifically affected during RNA virus infection by a high-throughput assay and further found that HFE was a key negative regulator of RIG-I-like receptors (RLR)-mediated type I interferons (IFNs) signaling. RNA virus infection inhibited the binding of HFE to MAVS (mitochondrial antiviral signaling protein) and blocked MAVS degradation via selective autophagy. HFE mediated MAVS autophagic degradation by binding to SQSTM1/p62. Depletion of Hfe abrogated the autophagic degradation of MAVS, leading to the stronger antiviral immune response. These findings established a novel regulatory role of selective autophagy in innate antiviral immune response by the iron metabolism-related gene Hfe. These data further provided insights into the crosstalk among iron metabolism, autophagy, and innate immune response. Abbreviations: ATG: autophagy-related; BAL: bronchoalveolar lavage fluid; BMDMs: bone marrow-derived macrophages; CGAS: cyclic GMP-AMP synthase; CQ: chloroquine; Dpi: days post-infection; ELISA: enzyme-linked immunosorbent assay; GFP: green fluorescent protein; HAMP: hepcidin antimicrobial peptide; Hpi: hours post-infection; HJV: hemojuvelin BMP co-receptor; IFNs: interferons; IL6: interleukin 6; IRF3: interferon regulatory factor 3; ISRE: interferon-stimulated response element; Lipo: clodronate liposomes; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MAVS: mitochondrial antiviral signaling protein; MEFs: mouse embryonic fibroblasts; SLC40A1/FPN1: solute carrier family 40 (iron-regulated transporter), member 1; flatiron; SQSTM1/p62: sequestosome 1; STAT1: signal transducer and activator of transcription 1; STING1/STING: stimulator of interferon response cGAMP interactor 1; TBK1: TANK-binding kinase 1; TFRC/TfR1: transferrin receptor; TNF/TNFα: tumor necrosis factor; WT: wild type. [ABSTRACT FROM AUTHOR]

A study was conducted to characterize the structure of pancreatic cholesterol esterase (CEase) at 1.6 Angstrom. The enzyme was assayed by observing the hydrolysis of cholesteryl oleate based on pH, enzyme concentration and taurocholate while Purified CEase was dialyzed against sodium phosphate for crystallization. Results indicated that increases in isoelectric forms and changes in mass-to-charge ratio in CEase were correlated with the presence of N-linked sugars.

Human pancreatic cholesterol esterase (CEase) has two structural regions that may be responsible for the regulation of uptake of cholesterol in humans. Heparin inhibits hydrolysis of cholesterol oleate catalyzed by human CEase to a larger extent than it inhibits the bovine enzyme. The C-terminal portion of the human CEase has 16 repeats of 11 amino acids each, rich in proline but the enzyme from other species has lesser repeats. Activities of the membrane-bound human and bovine enzymes are higher than the enzymes in solution.

[Display omitted] • A flue gas treatment efficiency of 280 L·h−1·kg−1 was achieved. • 97 % CO 2 can be captured and 95 % converted to CH 4 (∼100 % selectivity) • Synergy of adsorption and catalytic functionalities is pivotal. Massive emission of CO 2 is one of the main contributors to global warming, and CO 2 capture, utilization and storage (CCUS) was proposed as an indispensable option for carbon reduction. Recently, integrated carbon capture and conversion (ICCC) was regarded as a potential approach to lower the cost of CCUS, and development of efficient dual-functional materials (DFMs) is among the top priority in the area. Herein, Ni-MgO-Al 2 O 3 DFMs were prepared by calcination of Ni-impregnated Mg-Al layered double hydroxide (MgAl-LDH), as such, the pre-loaded Ni species was actively involved in the decomposition of MgAl-LDH, therefore the synergy and balance of the adsorption and catalytic functionalities for ICCC can be promoted in the resulted DFMs. The optimized sample (20NiMgAl(5 0 0)) showed excellent ICCC performance. At 300 °C, continuous abatement of CO 2 from simulated flue gas (15 vol.%CO 2 /N 2) was achieved with an excellent efficiency of 280 L·h−1·kg−1. Composition analysis of the effluent gas demonstrated that over 97 % CO 2 in the feed could be captured, and over 95 % of the captured CO 2 was converted to CH 4 with ∼ 100 % selectivity. Full retention of the adsorption capacity was observed for 10 adsorption-methanation cycles, and the post-reaction characterization of the DFMs indicated little structure change. This work may pave a way to the practical application of the ICCC technology, and also inspire other integration approaches between CO 2 capture and the following mitigation technologies. [ABSTRACT FROM AUTHOR]

Autophagy can be utilized by the influenza A virus (IAV) to facilitate its replication. However, whether autophagy is induced at the stage of IAV entry is still unclear. Here, we report that IAV induces autophagy by hemagglutinin (HA) binding to heat shock protein 90AA1 (HSP90AA1) distributed on the cell surface. Virus overlay protein binding assay and pull-down assay indicated that IAV HA bound directly to cell surface HSP90AA1. Knockdown of HSP90AA1 weakened H1N1 infection. Incubation of IAV viral particles with recombinant HSP90AA1 or prior blockade of A549 cells with an anti-HSP90AA1 antibody could inhibit attachment of IAV. Moreover, we found that recombinant HA1 protein binding to cell surface HSP90AA1 was sufficient to induce autophagy through the AKT-MTOR pathway. Our study reveals that the HSP90AA1 on cell surface participates in IAV entry by directing binding to the HA1 subunit of IAV and subsequently induces autophagy. [ABSTRACT FROM AUTHOR]

• Li-rich layered material Li 1.233 Mo 0.467 Fe 0.3 O 2 was synthesized by solid-state reaction method. • As-prepared Li 1.233 Mo 0.467 Fe 0.3 O 2 has high electrochemical performance. • The lithium storage mechanism of Li 1.233 Mo 0.467 Fe 0.3 O 2 is investigated by ex-situ XRD. Li-rich layered transition metal oxides show high reversible capacity in rechargeable lithium-ion batteries, as represented by the Li 1.2 Ni 0.13 Co 0.13 Mn 0.54 O 2. However, it suffers from severe problems in practical applications such as voltage fading, poor cycle life and poor rate performance. Here, we reported a Li-rich layered oxide Li 1.233 Mo 0.467 Fe 0.3 O 2 showing high reversible specific capacity of 262.9 mA h/g at the initial cycle and then up to 305.4 mA h/g after several cycles. The material shows decent capacity retention that after 25 cycles it still sustained a value of 257.8 mAh/g at the charge/discharge voltage range of 1.0–4.3 V. It is noticed that the capacity increase at 2.5 V platform is attributed to structural rearrangement at lower potential. The present work suggests choosing appropriate constituent stabilizing structures in Li-rich layered oxides should be an efficient strategy for designing advanced cathode materials. [ABSTRACT FROM AUTHOR]

The H7 subtype avian influenza virus (AIV) has been reported to infect not only poultry but also humans. The haemagglutinin (HA) protein is the major surface antigen of AIV and plays an important role in viral infection. In this study, five monoclonal antibodies (mAbs, 2F8, 3F6, 5C11, 5E2 and 5C12) against the HA protein of H7 virus were produced and characterized. Epitope mapping indicated that 103RESGSS107 was the minimal linear epitope recognized by the mAbs 2F8/3F6/5C11, and mAbs 5E2/5C12 recognized the epitope 103-145aa. The protein sequence alignment of HA indicated that the two epitopes were not found in other subtypes of AIV, and none of the five mAbs cross-reacted with other subtypes, suggesting these mAbs are specific to H7 virus. The epitope 103RESGSS107 was highly conserved among Eurasian lineage strains of H7 AIV, whereas three amino acid substitutions (E104R, E104K and E104G) in the epitope occurred in 98.44% of North-American lineage strains. Any of these single mutations prevented the mutated epitope from being recognized by mAbs 2F8/3F6/5C11; thus, these mAbs can distinguish between Eurasian and North-American lineages of H7 strains. Furthermore, the mAbs 2F8, 3F6 and 5C11 could be highly blocked with H7-positive serum in blocking assays, revealing that 103RESGSS107 may be a dominant epitope stimulating the production of antibodies during viral infection. These results may facilitate future investigations into the structure and function of HA protein, as well as surveillance and detection of H7 virus. RESEARCH HIGHLIGHTS Five mAbs against HA protein of H7 AIV were generated and characterized. Two novel epitopes 103RESGSS107 and 103-145aa were identified. The epitope 103RESGSS107 differs between Eurasian and North-American lineages. The mAbs 2F8, 3F6 and 5C11 could distinguish two lineages of H7 strains. [ABSTRACT FROM AUTHOR]

Emotions play a key role in human communication and public presentations. Human emotions are usually expressed through multiple modalities. Therefore, exploring multimodal emotions and their coherence is of great value for understanding emotional expressions in presentations and improving presentation skills. However, manually watching and studying presentation videos is often tedious and time-consuming. There is a lack of tool support to help conduct an efficient and in-depth multi-level analysis. Thus, in this paper, we introduce EmoCo, an interactive visual analytics system to facilitate efficient analysis of emotion coherence across facial, text, and audio modalities in presentation videos. Our visualization system features a channel coherence view and a sentence clustering view that together enable users to obtain a quick overview of emotion coherence and its temporal evolution. In addition, a detail view and word view enable detailed exploration and comparison from the sentence level and word level, respectively. We thoroughly evaluate the proposed system and visualization techniques through two usage scenarios based on TED Talk videos and interviews with two domain experts. The results demonstrate the effectiveness of our system in gaining insights into emotion coherence in presentations. [ABSTRACT FROM AUTHOR]

RIG-I-like receptors (RLRs), retinoic acid inducible gene I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5), are pattern recognition receptors through which cells initially sense pathogenic RNA and trigger interferon (IFN) signaling. Herein, we report that interferon induced protein 35 (IFI35) activates the ring finger protein 125 (RNF125)-UbcH5c-dependent degradation of RLRs and represses the recognition by RIG-I and MDA5 of viral RNA to inhibit innate immunity. Furthermore, IFI35 binds selectively to different subtypes of influenza A virus (IAV) nonstructural protein 1 (NS1) with asparagine residue207 (N207). Functionally, the NS1(N207)-IFI35 interaction restores the activity of RLRs, and IAV with NS1(non-N207) showed high pathogenicity in mice. Big data analysis showed that the 21st century pandemic IAV are almost all characterized by NS1 protein with non-N207. Collectively, our data uncovered the mechanism of IFI35 restricting the activation of RLRs and provides a new drug target comprising the NS1 protein of different IAV subtypes. • IFI35 inhibits RIG-I and MDA5 recognizing viral RNA. • IFI35 increases RNF125's E3 ligase activity by promoting its interaction with UbcH5c. • NS1 interferes with IFI35-mediated inhibition of RIG-I-like receptors signaling. • Amino acid 207 of NS1 is critical for its interaction with IFI35. • Mutations at amino acid 207 of NS1 are closely related to influenza A virus outbreaks. [ABSTRACT FROM AUTHOR]