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Bivalves are regulators of coastal lagoons and provide a wide range of ecosystem services. However, coastal lagoons are sensitive to climate change. Our objective was to describe the drivers of the cascade of ecological events that occurred during a summer heatwave and which resulted in recruitment failure of the Pacific oyster Crassostrea gigas. Results show that elevated temperatures and salinity caused a shift in planktonic food availability toward smaller taxa. These trophic changes did not affect food accumulation by oyster larvae or their fatty acid composition but did affect post-metamorphosis success, with up to 24% fewer young metamorphosed postlarvae at some sites and no development of juveniles at all sites. This resulted in the failure of oyster recruitment and in the development of tubeworms, a trophic and spatial competitor that can better ingest small particles. This knowledge suggests that, in the context of marine heatwaves, the ecological limits of oyster larvae are narrower than their physiological limits.

Background Although native to North America, the invasion of the aphid-like grape phylloxera Daktulosphaira vitifoliae across the globe altered the course of grape cultivation. For the past 150 years, viticulture relied on grafting-resistant North American Vitis species as rootstocks, thereby limiting genetic stocks tolerant to other stressors such as pathogens and climate change. Limited understanding of the insect genetics resulted in successive outbreaks across the globe when rootstocks failed. Here we report the 294-Mb genome of D. vitifoliae as a basic tool to understand host plant manipulation, nutritional endosymbiosis, and enhance global viticulture. Results Using a combination of genome, RNA, and population resequencing, we found grape phylloxera showed high duplication rates since its common ancestor with aphids, but similarity in most metabolic genes, despite lacking obligate nutritional symbioses and feeding from parenchyma. Similarly, no enrichment occurred in development genes in relation to viviparity. However, phylloxera evolved > 2700 unique genes that resemble putative effectors and are active during feeding. Population sequencing revealed the global invasion began from the upper Mississippi River in North America, spread to Europe and from there to the rest of the world. Conclusions The grape phylloxera genome reveals genetic architecture relative to the evolution of nutritional endosymbiosis, viviparity, and herbivory. The extraordinary expansion in effector genes also suggests novel adaptations to plant feeding and how insects induce complex plant phenotypes, for instance galls. Finally, our understanding of the origin of this invasive species and its genome provide genetics resources to alleviate rootstock bottlenecks restricting the advancement of viticulture.

An amendment to this paper has been published and can be accessed via the original article.

New in situ measurements to constrain the range, distribution, and spatial (meter-scale) variations of permeability in shallow crustal fault zones are reported based on systematic downhole tests at 0.5-km depth in crystalline rock. Single and cross-hole hydraulic packer tests were performed at a new dedicated test facility hosted in the Grimsel Test Site, in the Swiss Alps, following the technical instrumentation and isolation of discrete fault zones accessed by an array of boreholes. Single-hole test results are presented in this paper, while cross-hole experiments are reported in the companion paper. Our results reveal a sharp spatial falloff in permeability, from 10(-13) to 10(-21) m(2), with off-fault distances of 1-5 m and characterized by a power-law relation with fracture density. Fractures linking subparallel faults were detected as high-permeability discrete spots several meters away from off-fault damage. Due to the narrow (centimeter-wide) thickness of fault cores, the hydraulic tests presented in this study do not characterize the permeability of fault core materials. The transmissivity of single fractures spans six orders of magnitude (10(-12) to 10(-6) m(2)/s) and is systematically higher in damage zones. In situ stresses appear to have a minor effect on natural, present-day fracture transmissivity at the borehole scale. We suggest that the geometrical and topological properties of fracture systems instead tend to control the permeability of the shallow crustal faults studied.