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Your search keyword '"Rochaix, Jean-David"' showing total 280 results
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280 results on '"Rochaix, Jean-David"'

5. The translocon protein FtsHi1 is an ATP‐dependent DNA/RNA helicase that prevents R‐loop accumulation in chloroplasts.

Author

Chai, Xin, Wang, Xiushun, Rong, Liwei, Luo, Manfei, Yuan, Li, Li, Qiuxin, He, Baoye, Jiang, Jingjing, Ji, Daili, Ouyang, Min, Lu, Qingtao, Zhang, Lixin, Rochaix, Jean‐David, and Chi, Wei

Subjects
RNA helicase, CHLOROPLASTS, SINGLE-stranded DNA, DNA helicases, NUCLEIC acids, GENE expression
Abstract

Summary: R‐loops, three‐stranded nucleic acid structures consisting of a DNA: RNA hybrid and displaced single‐stranded DNA, play critical roles in gene expression and genome stability. How R‐loop homeostasis is integrated into chloroplast gene expression remains largely unknown.We found an unexpected function of FtsHi1, an inner envelope membrane‐bound AAA‐ATPase in chloroplast R‐loop homeostasis of Arabidopsis thaliana.Previously, this protein was shown to function as a component of the import motor complex for nuclear‐encoded chloroplast proteins. However, this study provides evidence that FtsHi1 is an ATP‐dependent helicase that efficiently unwinds both DNA–DNA and DNA–RNA duplexes, thereby preventing R‐loop accumulation. Over‐accumulation of R‐loops could impair chloroplast transcription but not necessarily genome integrity. The dual function of FtsHi1 in both protein import and chloroplast gene expression may be important to coordinate the biogenesis of nuclear‐ and chloroplast‐encoded subunits of multi‐protein photosynthetic complexes.This study suggests a mechanical link between protein import and R‐loop homeostasis in chloroplasts of higher plants. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Identification of bZIP Transcription Factors That Regulate the Development of Leaf Epidermal Cells in Arabidopsis thaliana by Single-Cell RNA Sequencing.

Author

Wu, Rui, Liu, Zhixin, Sun, Susu, Qin, Aizhi, Liu, Hao, Zhou, Yaping, Li, Weiqiang, Liu, Yumeng, Hu, Mengke, Yang, Jincheng, Rochaix, Jean-David, An, Guoyong, Herrera-Estrella, Luis, Tran, Lam-Son Phan, and Sun, Xuwu

Subjects
TRANSCRIPTION factors, RNA sequencing, ARABIDOPSIS thaliana, JASMONIC acid, PAVEMENTS, PLANT hormones
Abstract

Epidermal cells are the main avenue for signal and material exchange between plants and the environment. Leaf epidermal cells primarily include pavement cells, guard cells, and trichome cells. The development and distribution of different epidermal cells are tightly regulated by a complex transcriptional regulatory network mediated by phytohormones, including jasmonic acid, and transcription factors. How the fate of leaf epidermal cells is determined, however, is still largely unknown due to the diversity of cell types and the complexity of their regulation. Here, we characterized the transcriptional profiles of epidermal cells in 3-day-old true leaves of Arabidopsis thaliana using single-cell RNA sequencing. We identified two genes encoding BASIC LEUCINE-ZIPPER (bZIP) transcription factors, namely bZIP25 and bZIP53, which are highly expressed in pavement cells and early-stage meristemoid cells. Densities of pavement cells and trichome cells were found to increase and decrease, respectively, in bzip25 and bzip53 mutants, compared with wild-type plants. This trend was more pronounced in the presence of jasmonic acid, suggesting that these transcription factors regulate the development of trichome cells and pavement cells in response to jasmonic acid. [ABSTRACT FROM AUTHOR]

Published
2024
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21. Chloroplast retrograde signal regulates flowering

Author

Feng, Peiqiang, Guo, Hailong, Chi, Wei, Chai, Xin, Sun, Xuwu, Xu, Xiumei, Ma, Jinfang, Rochaix, Jean-David, Leister, Dario, Wang, Haiyang, Lu, Congming, and Zhang, Lixin

Published
2016

41. Chloroplast protein import machinery and quality control.

Author

Rochaix, Jean‐David

Subjects
*CHLOROPLASTS, *PROTEIN precursors, *PROTEOLYSIS, *QUALITY control, *PROTEIN folding, *CHLOROPLAST membranes, *PROTEINS, *PROTEASOMES
Abstract

Most chloroplast proteins are nucleus‐encoded, translated on cytoplasmic ribosomes as precursor proteins, and imported into chloroplasts through TOC and TIC, the translocons of the outer and inner chloroplast envelope membranes. While the composition of the TOC complex is well established, there is still some controversy about the importance of a recently identified TIC complex consisting of Tic20, Tic214, Tic100, and Tic56. TOC and TIC form a supercomplex with a protein channel at the junction of the outer and inner envelope membranes through which preproteins are pulled into the stroma by the ATP‐powered Ycf2 complex consisting of several FtsH‐like ATPases and/or by chloroplast Hsp proteins. Several components of the TOC/TIC system are moonlighting proteins with additional roles in chloroplast gene expression and metabolism. Chaperones and co‐chaperones, associated with TOC and TIC on the cytoplasmic and stromal side of the chloroplast envelope, participate in the unfolding and folding of the precursor proteins and act together with the ubiquitin–proteasome system in protein quality control. Chloroplast protein import is also intimately linked with retrograde signaling, revealing altogether an unsuspected complexity in the regulation of this process. [ABSTRACT FROM AUTHOR]

Published
2022
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