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2. Identification of a non-canonical chemokine-receptor pathway suppressing regulatory T cells to drive atherosclerosis

Author

Döring, Yvonne, van der Vorst, Emiel P. C., Yan, Yi, Neideck, Carlos, Blanchet, Xavier, Jansen, Yvonne, Kemmerich, Manuela, Bayasgalan, Soyolmaa, Peters, Linsey J. F., Hristov, Michael, Bidzhekov, Kiril, Yin, Changjun, Zhang, Xi, Leberzammer, Julian, Li, Ya, Park, Inhye, Kral, Maria, Nitz, Katrin, Parma, Laura, Gencer, Selin, Habenicht, Andreas J. R., Faussner, Alexander, Teupser, Daniel, Monaco, Claudia, Holdt, Lesca, Megens, Remco T. A., Atzler, Dorothee, Santovito, Donato, von Hundelshausen, Philipp, and Weber, Christian

Published
2024
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5. Single cell spatial analysis reveals inflammatory foci of immature neutrophil and CD8 T cells in COVID-19 lungs

Author

Weeratunga, Praveen, Denney, Laura, Bull, Joshua A., Repapi, Emmanouela, Sergeant, Martin, Etherington, Rachel, Vuppussetty, Chaitanya, Turner, Gareth D. H., Clelland, Colin, Woo, Jeongmin, Cross, Amy, Issa, Fadi, de Andrea, Carlos Eduardo, Melero Bermejo, Ignacio, Sims, David, McGowan, Simon, Zurke, Yasemin-Xiomara, Ahern, David J., Gamez, Eddie C., Whalley, Justin, Richards, Duncan, Klenerman, Paul, Monaco, Claudia, Udalova, Irina A., Dong, Tao, Antanaviciute, Agne, Ogg, Graham, Knight, Julian C., Byrne, Helen M., Taylor, Stephen, and Ho, Ling-Pei

Published
2023
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6. Lipid-associated macrophages transition to an inflammatory state in human atherosclerosis, increasing the risk of cerebrovascular complications

Author

Dib, Lea, Koneva, Lada A., Edsfeldt, Andreas, Zurke, Yasemin-Xiomara, Sun, Jiangming, Nitulescu, Mihaela, Attar, Moustafa, Lutgens, Esther, Schmidt, Steffen, Lindholm, Marie W., Choudhury, Robin P., Cassimjee, Ismail, Lee, Regent, Handa, Ashok, Goncalves, Isabel, Sansom, Stephen N., and Monaco, Claudia

Published
2023
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8. Transcriptomic-based clustering of human atherosclerotic plaques identifies subgroups with different underlying biology and clinical presentation

Author

Mokry, Michal, Boltjes, Arjan, Slenders, Lotte, Bel-Bordes, Gemma, Cui, Kai, Brouwer, Eli, Mekke, Joost M., Depuydt, Marie A. C., Timmerman, Nathalie, Waissi, Farahnaz, Verwer, Maarten C., Turner, Adam W., Khan, Mohammad Daud, Hodonsky, Chani J., Diez Benavente, Ernest, Hartman, Robin J. G., van den Dungen, Noortje A. M., Lansu, Nico, Nagyova, Emilia, Prange, Koen H. M., Kovacic, Jason C., Björkegren, Johan L. M., Pavlos, Eleftherios, Andreakos, Evangelos, Schunkert, Heribert, Owens, Gary K., Monaco, Claudia, Finn, Aloke V., Virmani, Renu, Leeper, Nicholas J., de Winther, Menno P. J., Kuiper, Johan, de Borst, Gert J., Stroes, Erik S. G., Civelek, Mete, de Kleijn, Dominique P. V., den Ruijter, Hester M., Asselbergs, Folkert W., van der Laan, Sander W., Miller, Clint L., and Pasterkamp, Gerard

Published
2022
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11. Author Correction: Lipid-associated macrophages transition to an inflammatory state in human atherosclerosis, increasing the risk of cerebrovascular complications

Author

Dib, Lea, Koneva, Lada A., Edsfeldt, Andreas, Zurke, Yasemin-Xiomara, Sun, Jiangming, Nitulescu, Mihaela, Attar, Moustafa, Lutgens, Esther, Schmidt, Steffen, Lindholm, Marie W., Choudhury, Robin P., Cassimjee, Ismail, Lee, Regent, Handa, Ashok, Goncalves, Isabel, Sansom, Stephen N., and Monaco, Claudia

Published
2023
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12. Publisher Correction: Lipid-associated macrophages transition to an inflammatory state in human atherosclerosis, increasing the risk of cerebrovascular complications

Author

Dib, Lea, Koneva, Lada A., Edsfeldt, Andreas, Zurke, Yasemin-Xiomara, Sun, Jiangming, Nitulescu, Mihaela, Attar, Moustafa, Lutgens, Esther, Schmidt, Steffen, Lindholm, Marie W., Choudhury, Robin P., Cassimjee, Ismail, Lee, Regent, Handa, Ashok, Goncalves, Isabel, Sansom, Stephen N., and Monaco, Claudia

Published
2023
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14. Toll-like Receptor 3 Is a Therapeutic Target for Pulmonary Hypertension

Author

Farkas, Daniela, Thompson, AA Roger, Bhagwani, Aneel R, Hultman, Schuyler, Ji, Hyun, Kotha, Naveen, Farr, Grant, Arnold, Nadine D, Braithwaite, Adam, Casbolt, Helen, Cole, Jennifer E, Sabroe, Ian, Monaco, Claudia, Cool, Carlyne D, Goncharova, Elena A, Lawrie, Allan, and Farkas, Laszlo

Subjects
Rare Diseases, Lung, Cardiovascular, Animals, Disease Models, Animal, Humans, Hypertension, Pulmonary, Mice, Rats, Signal Transduction, Toll-Like Receptor 3, pulmonary hypertension, toll-like receptor 3, endothelial cell, double-stranded RNA, apoptosis, Medical and Health Sciences, Respiratory System
Abstract

RationalePulmonary arterial hypertension (PAH) is characterized by vascular cell proliferation and endothelial cell apoptosis. TLR3 (Toll-like receptor 3) is a receptor for double-stranded RNA and has been recently implicated in vascular protection.ObjectivesTo study the expression and role of TLR3 in PAH and to determine whether a TLR3 agonist reduces pulmonary hypertension in preclinical models.MethodsLung tissue and endothelial cells from patients with PAH were investigated by polymerase chain reaction, immunofluorescence, and apoptosis assays. TLR3-/- and TLR3+/+ mice were exposed to chronic hypoxia and SU5416. Chronic hypoxia or chronic hypoxia/SU5416 rats were treated with the TLR3 agonist polyinosinic/polycytidylic acid (Poly[I:C]).Measurements and main resultsTLR3 expression was reduced in PAH patient lung tissue and endothelial cells, and TLR3-/- mice exhibited more severe pulmonary hypertension following exposure to chronic hypoxia/SU5416. TLR3 knockdown promoted double-stranded RNA signaling via other intracellular RNA receptors in endothelial cells. This was associated with greater susceptibility to apoptosis, a known driver of pulmonary vascular remodeling. Poly(I:C) increased TLR3 expression via IL-10 in rat endothelial cells. In vivo, high-dose Poly(I:C) reduced pulmonary hypertension in both rat models in proof-of-principle experiments. In addition, Poly(I:C) also reduced right ventricular failure in established pulmonary hypertension.ConclusionsOur work identifies a novel role for TLR3 in PAH based on the findings that reduced expression of TLR3 contributes to endothelial apoptosis and pulmonary vascular remodeling.

Published
2019

15. Constructing custom-made radiotranscriptomic signatures of vascular inflammation from routine CT angiograms: a prospective outcomes validation study in COVID-19

Author

Ahern, David J, Ai, Zhichao, Ainsworth, Mark, Allan, Chris, Allcock, Alice, Angus, Brian, Ansari, M Azim, Arancibia-Cárcamo, Carolina, Aschenbrenner, Dominik, Attar, Moustafa, Baillie, J Kenneth, Barnes, Eleanor, Bashford-Rogers, Rachael, Bashyal, Archana, Beer, Sally, Berridge, Georgina, Beveridge, Amy, Bibi, Sagida, Bicanic, Tihana, Blackwell, Luke, Bowness, Paul, Brent, Andrew, Brown, Andrew, Broxholme, John, Buck, David, Burnham, Katie, Byrne, Helen, Camara, Susana, Ferreira, Ivan Candido, Charles, Philip, Chen, Wentao, Chen, Yi-Ling, Chong, Amanda, Clutterbuck, Elizabeth, Coles, Mark, Conlon, Christopher, Cornall, Richard, Cribbs, Adam, Curion, Fabiola, Davenport, Emma, Davidson, Neil, Davis, Simon, Dendrou, Calliope, Dequaire, Julie, Dib, Lea, Docker, James, Dold, Christina, Dong, Tao, Downes, Damien, Drakesmith, Hal, Dunachie, Susanna, Duncan, David, Eijsbouts, Chris, Esnouf, Robert, Espinosa, Alexis, Etherington, Rachel, Fairfax, Benjamin, Fairhead, Rory, Fang, Hai, Fassih, Shayan, Felle, Sally, Fernandez Mendoza, Maria, Ferreira, Ricardo, Fischer, Roman, Foord, Thomas, Forrow, Aden, Frater, John, Fries, Anastasia, Gallardo Sanchez, Veronica, Garner, Lucy, Geeves, Clementine, Georgiou, Dominique, Godfrey, Leila, Golubchik, Tanya, Gomez Vazquez, Maria, Green, Angie, Harper, Hong, Harrington, Heather, Heilig, Raphael, Hester, Svenja, Hill, Jennifer, Hinds, Charles, Hird, Clare, Ho, Ling-Pei, Hoekzema, Renee, Hollis, Benjamin, Hughes, Jim, Hutton, Paula, Jackson-Wood, Matthew, Jainarayanan, Ashwin, James-Bott, Anna, Jansen, Kathrin, Jeffery, Katie, Jones, Elizabeth, Jostins, Luke, Kerr, Georgina, Kim, David, Klenerman, Paul, Knight, Julian, Kumar, Vinod, Kumar Sharma, Piyush, Kurupati, Prathiba, Kwok, Andrew, Lee, Angela, Linder, Aline, Lockett, Teresa, Lonie, Lorne, Lopopolo, Maria, Lukoseviciute, Martyna, Luo, Jian, Marinou, Spyridoula, Marsden, Brian, Martinez, Jose, Matthews, Philippa, Mazurczyk, Michalina, McGowan, Simon, McKechnie, Stuart, Mead, Adam, Mentzer, Alexander, Mi, Yuxin, Monaco, Claudia, Montadon, Ruddy, Napolitani, Giorgio, Nassiri, Isar, Novak, Alex, O'Brien, Darragh, O'Connor, Daniel, O'Donnell, Denise, Ogg, Graham, Overend, Lauren, Park, Inhye, Pavord, Ian, Peng, Yanchun, Penkava, Frank, Pereira Pinho, Mariana, Perez, Elena, Pollard, Andrew, Powrie, Fiona, Psaila, Bethan, Quan, T Phuong, Repapi, Emmanouela, Revale, Santiago, Silva-Reyes, Laura, Richard, Jean-Baptiste, Rich-Griffin, Charlotte, Ritter, Thomas, Rollier, Christine, Rowland, Matthew, Ruehle, Fabian, Salio, Mariolina, Sansom, Stephen Nicholas, Sanches Peres, Raphael, Santos Delgado, Alberto, Sauka-Spengler, Tatjana, Schwessinger, Ron, Scozzafava, Giuseppe, Screaton, Gavin, Seigal, Anna, Semple, Malcolm, Sergeant, Martin, Simoglou Karali, Christina, Sims, David, Skelly, Donal, Slawinski, Hubert, Sobrinodiaz, Alberto, Sousos, Nikolaos, Stafford, Lizzie, Stockdale, Lisa, Strickland, Marie, Sumray, Otto, Sun, Bo, Taylor, Chelsea, Taylor, Stephen, Taylor, Adan, Thongjuea, Supat, Thraves, Hannah, Todd, John, Tomic, Adriana, Tong, Orion, Trebes, Amy, Trzupek, Dominik, Tucci, Felicia Anna, Turtle, Lance, Udalova, Irina, Uhlig, Holm, van Grinsven, Erinke, Vendrell, Iolanda, Verheul, Marije, Voda, Alexandru, Wang, Guanlin, Wang, Lihui, Wang, Dapeng, Watkinson, Peter, Watson, Robert, Weinberger, Michael, Whalley, Justin, Witty, Lorna, Wray, Katherine, Xue, Luzheng, Yuen Yeung, Hing, Yin, Zixi, Young, Rebecca, Youngs, Jonathan, Zhang, Ping, Zurke, Yasemin-Xiomara, Banning, Adrian, Antonopoulos, Alexios, Bajaj, Amrita, Kelion, Andrew, Deshpande, Aparna, Kardos, Attila, Hudson, Benjamin, Koo, Bon-Kwon, Shirodaria, Cheerag, Xie, Cheng, Kotanidis, Christos, Mahon, Ciara, Berry, Colin, Adlam, David, Newby, David, Connolly, Derek, Scaletta, Diane, Alexander, Donna, Nicol, Ed, McAlindon, Elisa, Oikonomou, Evangelos, Pugliese, Francesca, Pontone, Gianluca, Benedetti, Giulia, He, Guo-Wei, West, Henry, Kondo, Hidekazu, Benedek, Imre, Das, Intrajeet, Deanfield, John, Graby, John, Greenwood, John, Rodrigues, Jonathan, Ge, Junbo, Channon, Keith, Fabritz, Larissa, Fan, Li-Juan, Kingham, Lucy, Guglielmo, Marco, Lyasheva, Maria, Schmitt, Matthias, Beer, Meinrad, Anderson, Michelle, Desai, Milind, Marwan, Mohamed, Takahashi, Naohiko, Mehta, Nehal, Dai, Neng, Screaton, Nicholas, Sabharwal, Nikant, Maurovich-Horvat, Pál, Rao, Praveen, Kotronias, Rafail, Kharbanda, Rajesh, Preston, Rebecca, Wood, Richard, Blankstein, Ron, Rajani, Ronak, Mirsadraee, Saeed, Munir, Shahzad, Thomas, Sheena, Neubauer, Stefan, Klömpken, Steffen, Petersen, Steffen, Achenbach, Stephan, Anthony, Susan, Mak, Sze, Mittal, Tarun, Benedek, Theodora, Sharma, Vinoda, Lin, Wen-Hua, Kotanidis, Christos P, Rodrigues, Jonathan C L, O’Connor, Daniel, Siddique, Muhammad, Lockstone, Helen, Oikonomou, Evangelos K, Badi, Ileana, Lumley, Sheila F, Constantinides, Bede, Sanderson, Nicholas, Rodger, Gillian, Chau, Kevin K, Lodge, Archie, Tsakok, Maria, Gleeson, Fergus, Indrajeet, Das, Hudson, Benjamin J, Srivastava, Vivek, Farid, Shakil, Krasopoulos, George, Sayeed, Rana, Newby, David E, Channon, Keith M, and Antoniades, Charalambos

Published
2022
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18. The extracellular heparan sulfatase SULF2 limits myeloid IFNβ signaling and Th17 responses in inflammatory arthritis

Author

Swart, Maarten, primary, Redpath, Andia, additional, Ogbechi, Joy, additional, Cardenas, Ryan, additional, Topping, Louise, additional, Compeer, Ewoud B., additional, Goddard, Michael, additional, Chanalaris, Anastasios, additional, Williams, Richard, additional, Brewer, Daniel S., additional, Smart, Nicola, additional, Monaco, Claudia, additional, and Troeberg, Linda, additional

Published
2024
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21. Stimulation of the α7 Nicotinic Acetylcholine Receptor Protects against Neuroinflammation after Tibia Fracture and Endotoxemia in Mice

Author

Terrando, Niccolò, Yang, Ting, Ryu, Jae Kyu, Newton, Phillip T, Monaco, Claudia, Feldmann, Marc, Ma, Daqing, Akassoglou, Katerina, and Maze, Mervyn

Subjects
Medicinal and Biomolecular Chemistry, Chemical Sciences, Neurosciences, Infectious Diseases, Patient Safety, Brain Disorders, 2.1 Biological and endogenous factors, Aetiology, Animals, Antigens, CD, Antigens, Differentiation, Myelomonocytic, Aza Compounds, CD11b Antigen, Cell Line, Cells, Cultured, Cholinergic Agonists, Cognition Disorders, Cytokines, Dioxins, Endotoxemia, Fractures, Bone, Hippocampus, Lipopolysaccharides, Macrophages, Male, Memory, Mice, Inbred C57BL, NF-kappa B, Postoperative Complications, Rats, Tibia, alpha7 Nicotinic Acetylcholine Receptor, Biochemistry and Cell Biology, Immunology, Medicinal and biomolecular chemistry
Abstract

Surgery and critical illness often associate with cognitive decline. Surgical trauma or infection can lead independently to learning and memory impairments via similar, but not identical, cellular signaling of the innate immune system that promotes neuroinflammation. In this study we explored the putative synergism between aseptic orthopedic surgery and infection, the latter reproduced by postoperative lipopolysaccharide (LPS) administration. We observed that surgery and LPS augmented systemic inflammation up to postoperative d 3 and this was associated with further neuroinflammation (CD11b and CD68 immunoreactivity) in the hippocampus in mice compared with those receiving surgery or LPS alone. Administration of a selective α7 subtype nicotinic acetylcholine receptor (α7 nAChR) agonist 2 h after LPS significantly improved neuroinflammation and hippocampal-dependent memory dysfunction. Modulation of nuclear factor-kappa B (NF-κB) activation in monocytes and regulation of the oxidative stress response through nicotinamide adenine dinucleotide phosphate (NADPH) signaling appear to be key targets in modulating this response. Overall, these results suggest that it may be conceivable to limit and possibly prevent postoperative complications, including cognitive decline and/or infections, through stimulation of the cholinergic antiinflammatory pathway.

Published
2014

22. Posicionamento do Departamento de Imagem Cardiovascular da Sociedade Brasileira de Cardiologia sobre o Uso do Strain Miocárdico na Rotina do Cardiologista – 2023

Author

Almeida, André Luiz Cerqueira, primary, Melo, Marcelo Dantas Tavares de, additional, Bihan, David Costa de Souza Le, additional, Vieira, Marcelo Luiz Campos, additional, Pena, José Luiz Barros, additional, Del Castillo, José Maria, additional, Abensur, Henry, additional, Hortegal, Renato de Aguiar, additional, Otto, Maria Estefania Bosco, additional, Piveta, Rafael Bonafim, additional, Dantas, Maria Rosa, additional, Assef, Jorge Eduardo, additional, Beck, Adenalva Lima de Souza, additional, Santo, Thais Harada Campos Espirito, additional, Silva, Tonnison de Oliveira, additional, Salemi, Vera Maria Cury, additional, Rocon, Camila, additional, Lima, Márcio Silva Miguel, additional, Barberato, Silvio Henrique, additional, Rodrigues, Ana Clara, additional, Rabschkowisky, Arnaldo, additional, Frota, Daniela do Carmo Rassi, additional, Gripp, Eliza de Almeida, additional, Barretto, Rodrigo Bellio de Mattos, additional, Silva, Sandra Marques e, additional, Cauduro, Sanderson Antonio, additional, Pinheiro, Aurélio Carvalho, additional, Araujo, Salustiano Pereira de, additional, Tressino, Cintia Galhardo, additional, Silva, Carlos Eduardo Suaide, additional, Monaco, Claudia Gianini, additional, Paiva, Marcelo Goulart, additional, Fisher, Cláudio Henrique, additional, Alves, Marco Stephan Lofrano, additional, Grau, Cláudia R. Pinheiro de Castro, additional, Santos, Maria Veronica Camara dos, additional, Guimarães, Isabel Cristina Britto, additional, Morhy, Samira Saady, additional, Leal, Gabriela Nunes, additional, Soares, Andressa Mussi, additional, Cruz, Cecilia Beatriz Bittencourt Viana, additional, Guimarães, Fabio Villaça, additional, Assunção, Bruna Morhy Borges Leal, additional, Fernandes, Rafael Modesto, additional, Saraiva, Roberto Magalhães, additional, Tsutsui, Jeane Mike, additional, Soares, Fábio Luis de Jesus, additional, Falcão, Sandra Nívea dos Reis Saraiva, additional, Hotta, Viviane Tiemi, additional, Armstrong, Anderson da Costa, additional, Hygidio, Daniel de Andrade, additional, Miglioranza, Marcelo Haertel, additional, Camarozano, Ana Cristina, additional, Lopes, Marly Maria Uellendahl, additional, Cerci, Rodrigo Julio, additional, Siqueira, Maria Eduarda Menezes de, additional, Torreão, Jorge Andion, additional, Rochitte, Carlos Eduardo, additional, and Felix, Alex, additional

Published
2023
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25. The impact of IL-1 modulation on the development of lipopolysaccharide-induced cognitive dysfunction

Author

Terrando, Niccolò, Rei Fidalgo, António, Vizcaychipi, Marcela, Cibelli, Mario, Ma, Daqing, Monaco, Claudia, Feldmann, Marc, and Maze, Mervyn

Abstract

Abstract Introduction The impact of pro-inflammatory cytokines on neuroinflammation and cognitive function after lipopolysaccharide (LPS) challenge remains elusive. Herein we provide evidence that there is a temporal correlation between high-mobility group box 1 (HMGB-1), microglial activation, and cognitive dysfunction. Disabling the interleukin (IL)-1 signaling pathway is sufficient to reduce inflammation and ameliorate the disability. Methods Endotoxemia was induced in wild-type and IL-1R-/- mice by intra peritoneal injection of E. Coli LPS (1 mg/kg). Markers of inflammation were assessed both peripherally and centrally, and correlated to behavioral outcome using trace fear conditioning. Results Increase in plasma tumor necrosis factor-α (TNFα) peaked at 30 minutes after LPS challenge. Up-regulation of IL-1β, IL-6 and HMGB-1 was more persistent, with detectable levels up to day three. A 15-fold increase in IL-6 and a 6.5-fold increase in IL-1β mRNA at 6 hours post intervention (P < 0.001 respectively) was found in the hippocampus. Reactive microgliosis was observed both at days one and three, and was associated with elevated HMGB-1 and impaired memory retention (P < 0.005). Preemptive administration of IL-1 receptor antagonist (IL-1Ra) significantly reduced plasma cytokines and hippocampal microgliosis and ameliorated cognitive dysfunction without affecting HMGB-1 levels. Similar results were observed in LPS-challenged mice lacking the IL-1 receptor to those seen in LPS-challenged wild type mice treated with IL-1Ra. Conclusions These data suggest that by blocking IL-1 signaling, the inflammatory cascade to LPS is attenuated, thereby reducing microglial activation and preventing the behavioral abnormality.

Published
2010

28. Lipoproteins act as vehicles for lipid antigen delivery and activation of invariant natural killer T cells

Author

Engelen, Suzanne E., primary, Ververs, Francesca A., additional, Markovska, Angela, additional, Lagerholm, B. Christoffer, additional, Kraaijenhof, Jordan M., additional, Yousif, Laura I.E., additional, Zurke, Yasemin-Xiomara, additional, Gulersonmez, Can M.C., additional, Kooijman, Sander, additional, Goddard, Michael, additional, van Eijkeren, Robert J., additional, Jervis, Peter J., additional, Besra, Gurdyal S., additional, Haitjema, Saskia, additional, Asselbergs, Folkert W., additional, Kalkhoven, Eric, additional, Ploegh, Hidde L., additional, Boes, Marianne, additional, Cerundolo, Vincenzo, additional, Hovingh, G.K., additional, Salio, Mariolina, additional, Stigter, Edwin C.A., additional, Rensen, Patrick C.N., additional, Monaco, Claudia, additional, and Schipper, Henk S., additional

Published
2023
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30. Pyruvate dehydrogenase kinase regulates vascular inflammation in atherosclerosis and increases cardiovascular risk

Author

Forteza, Maria J, primary, Berg, Martin, additional, Edsfeldt, Andreas, additional, Sun, Jangming, additional, Baumgartner, Roland, additional, Kareinen, Ilona, additional, Casagrande, Felipe Beccaria, additional, Hedin, Ulf, additional, Zhang, Song, additional, Vuckovic, Ivan, additional, Dzeja, Petras P, additional, Polyzos, Konstantinos A, additional, Gisterå, Anton, additional, Trauelsen, Mette, additional, Schwartz, Thue W, additional, Dib, Lea, additional, Herrmann, Joerg, additional, Monaco, Claudia, additional, Matic, Ljubica, additional, Gonçalves, Isabel, additional, and Ketelhuth, Daniel F J, additional

Published
2023
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32. Translational opportunities of single-cell biology in atherosclerosis

Author

de Winther, Menno P. J., Back, Magnus, Evans, Paul, Gomez, Delphine, Goncalves, Isabel, Jorgensen, Helle F., Koenen, Rory R., Lutgens, Esther, Norata, Giuseppe Danilo, Osto, Elena, Dib, Lea, Simons, Michael, Stellos, Konstantinos, Yla-Herttuala, Seppo, Winkels, Holger, Bochaton-Piallat, Marie-Luce, Monaco, Claudia, de Winther, Menno P. J., Back, Magnus, Evans, Paul, Gomez, Delphine, Goncalves, Isabel, Jorgensen, Helle F., Koenen, Rory R., Lutgens, Esther, Norata, Giuseppe Danilo, Osto, Elena, Dib, Lea, Simons, Michael, Stellos, Konstantinos, Yla-Herttuala, Seppo, Winkels, Holger, Bochaton-Piallat, Marie-Luce, and Monaco, Claudia

Abstract

The advent of single-cell biology opens a new chapter for understanding human biological processes and for diagnosing, monitoring, and treating disease. This revolution now reaches the field of cardiovascular disease (CVD). New technologies to interrogate CVD samples at single-cell resolution are allowing the identification of novel cell communities that are important in shaping disease development and direct towards new therapeutic strategies. These approaches have begun to revolutionize atherosclerosis pathology and redraw our understanding of disease development. This review discusses the state-of-the-art of single-cell analysis of atherosclerotic plaques, with a particular focus on human lesions, and presents the current resolution of cellular subpopulations and their heterogeneity and plasticity in relation to clinically relevant features. Opportunities and pitfalls of current technologies as well as the clinical impact of single-cell technologies in CVD patient care are highlighted, advocating for multidisciplinary and international collaborative efforts to join the cellular dots of CVD.

Published
2023

33. Cell selectivity in succinate receptor SUCNR1/GPR91 signaling in skeletal muscle

Author

Abdelmoez, Ahmed M., Dmytriyeva, Oksana, Zurke, Yasemin Xiomara, Trauelsen, Mette, Marica, Alesandra A., Savikj, Mladen, Smith, Jonathon A.B., Monaco, Claudia, Schwartz, Thue W., Krook, Anna, Pillon, Nicolas J., Abdelmoez, Ahmed M., Dmytriyeva, Oksana, Zurke, Yasemin Xiomara, Trauelsen, Mette, Marica, Alesandra A., Savikj, Mladen, Smith, Jonathon A.B., Monaco, Claudia, Schwartz, Thue W., Krook, Anna, and Pillon, Nicolas J.

Abstract

Succinate is released by skeletal muscle during exercise and activates SUCNR1/GPR91. Signaling of SUCNR1 is involved in metabolite-sensing paracrine communication in skeletal muscle during exercise. However, the specific cell types responding to succinate and the directionality of communication are unclear. We aim to characterize the expression of SUCNR1 in human skeletal muscle. De novo analysis of transcriptomic datasets demonstrated that SUCNR1 mRNA is expressed in immune, adipose, and liver tissues, but scarce in skeletal muscle. In human tissues, SUCNR1 mRNA was associated with macrophage markers. Single-cell RNA sequencing and fluorescent RNAscope demonstrated that in human skeletal muscle, SUCNR1 mRNA is not expressed in muscle fibers but coincided with macrophage populations. Human M2-polarized macrophages exhibit high levels of SUCNR1 mRNA and stimulation with selective agonists of SUCNR1 triggered Gq- and Gi-coupled signaling. Primary human skeletal muscle cells were unresponsive to SUCNR1 agonists. In conclusion, SUCNR1 is not expressed in muscle cells and its role in the adaptive response of skeletal muscle to exercise is most likely mediated via paracrine mechanisms involving M2-like macrophages within the muscle.NEW & NOTEWORTHY Macrophages but not skeletal muscle cells respond to extracellular succinate via SUCNR1/GPR91.

Published
2023

34. Lipoproteins act as vehicles for lipid antigen delivery and activation of invariant natural killer T-cells

Author

Infection & Immunity, CTI Boes, CDL Cluster Speciële Diagnostiek, Circulatory Health, Team Medisch, Zorg en O&O, Child Health, CMM Groep Burgering, Cancer, Cardiologie patientenzorg, Engelen, Suzanne E, Ververs, Francesca A, Markovska, Angela, Lagerholm, B Christoffer, Kraaijenhof, Jordan M, Yousif, Laura Ie, Zurke, Yasemin-Xiomara, Gulersonmez, Can Mc, Kooijman, Sander, Goddard, Michael, van Eijkeren, Robert J, Jervis, Peter J, Besra, Gurdyal S, Haitjema, Saskia, Asselbergs, Folkert W, Kalkhoven, Eric, Ploegh, Hidde L, Boes, Marianne, Cerundolo, Vincenzo, Hovingh, G Kees, Salio, Mariolina, Stigter, Edwin Ca, Rensen, Patrick Cn, Monaco, Claudia, Schipper, Henk S, Infection & Immunity, CTI Boes, CDL Cluster Speciële Diagnostiek, Circulatory Health, Team Medisch, Zorg en O&O, Child Health, CMM Groep Burgering, Cancer, Cardiologie patientenzorg, Engelen, Suzanne E, Ververs, Francesca A, Markovska, Angela, Lagerholm, B Christoffer, Kraaijenhof, Jordan M, Yousif, Laura Ie, Zurke, Yasemin-Xiomara, Gulersonmez, Can Mc, Kooijman, Sander, Goddard, Michael, van Eijkeren, Robert J, Jervis, Peter J, Besra, Gurdyal S, Haitjema, Saskia, Asselbergs, Folkert W, Kalkhoven, Eric, Ploegh, Hidde L, Boes, Marianne, Cerundolo, Vincenzo, Hovingh, G Kees, Salio, Mariolina, Stigter, Edwin Ca, Rensen, Patrick Cn, Monaco, Claudia, and Schipper, Henk S

Published
2023

35. Translational opportunities of single-cell biology in atherosclerosis

Author

de Winther, Menno P J; https://orcid.org/0000-0002-4038-6636, Bäck, Magnus; https://orcid.org/0000-0003-0853-5141, Evans, Paul C; https://orcid.org/0000-0001-7975-681X, Gomez, Delphine; https://orcid.org/0000-0002-3880-6961, Goncalves, Isabel; https://orcid.org/0000-0002-2935-0181, Jørgensen, Helle F; https://orcid.org/0000-0002-7909-2977, Koenen, Rory R; https://orcid.org/0000-0002-9955-9730, Lutgens, Esther; https://orcid.org/0000-0002-2609-5744, Norata, Giuseppe Danilo; https://orcid.org/0000-0002-6081-1257, Osto, Elena; https://orcid.org/0000-0001-8196-5696, Dib, Lea; https://orcid.org/0000-0001-5889-4738, Simons, Michael; https://orcid.org/0000-0003-0348-7734, Stellos, Konstantinos; https://orcid.org/0000-0002-0194-0825, Ylä-Herttuala, Seppo; https://orcid.org/0000-0001-7593-2708, Winkels, Holger; https://orcid.org/0000-0003-1333-2031, Bochaton-Piallat, Marie-Luce; https://orcid.org/0000-0001-8408-8932, Monaco, Claudia; https://orcid.org/0000-0003-1985-4914, de Winther, Menno P J; https://orcid.org/0000-0002-4038-6636, Bäck, Magnus; https://orcid.org/0000-0003-0853-5141, Evans, Paul C; https://orcid.org/0000-0001-7975-681X, Gomez, Delphine; https://orcid.org/0000-0002-3880-6961, Goncalves, Isabel; https://orcid.org/0000-0002-2935-0181, Jørgensen, Helle F; https://orcid.org/0000-0002-7909-2977, Koenen, Rory R; https://orcid.org/0000-0002-9955-9730, Lutgens, Esther; https://orcid.org/0000-0002-2609-5744, Norata, Giuseppe Danilo; https://orcid.org/0000-0002-6081-1257, Osto, Elena; https://orcid.org/0000-0001-8196-5696, Dib, Lea; https://orcid.org/0000-0001-5889-4738, Simons, Michael; https://orcid.org/0000-0003-0348-7734, Stellos, Konstantinos; https://orcid.org/0000-0002-0194-0825, Ylä-Herttuala, Seppo; https://orcid.org/0000-0001-7593-2708, Winkels, Holger; https://orcid.org/0000-0003-1333-2031, Bochaton-Piallat, Marie-Luce; https://orcid.org/0000-0001-8408-8932, and Monaco, Claudia; https://orcid.org/0000-0003-1985-4914

Abstract

The advent of single-cell biology opens a new chapter for understanding human biological processes and for diagnosing, monitoring, and treating disease. This revolution now reaches the field of cardiovascular disease (CVD). New technologies to interrogate CVD samples at single-cell resolution are allowing the identification of novel cell communities that are important in shaping disease development and direct towards new therapeutic strategies. These approaches have begun to revolutionize atherosclerosis pathology and redraw our understanding of disease development. This review discusses the state-of-the-art of single-cell analysis of atherosclerotic plaques, with a particular focus on human lesions, and presents the current resolution of cellular subpopulations and their heterogeneity and plasticity in relation to clinically relevant features. Opportunities and pitfalls of current technologies as well as the clinical impact of single-cell technologies in CVD patient care are highlighted, advocating for multidisciplinary and international collaborative efforts to join the cellular dots of CVD.

Published
2023

37. Translational opportunities of single-cell biology in atherosclerosis

Author

de Winther, Menno P J, primary, Bäck, Magnus, additional, Evans, Paul, additional, Gomez, Delphine, additional, Goncalves, Isabel, additional, Jørgensen, Helle F, additional, Koenen, Rory R, additional, Lutgens, Esther, additional, Norata, Giuseppe Danilo, additional, Osto, Elena, additional, Dib, Lea, additional, Simons, Michael, additional, Stellos, Konstantinos, additional, Ylä-Herttuala, Seppo, additional, Winkels, Holger, additional, Bochaton-Piallat, Marie-Luce, additional, and Monaco, Claudia, additional

Published
2022
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38. Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques

Author

Langley, Sarah R., Willeit, Karin, Didangelos, Athanasios, Matic, Ljubica Perisic, Skroblin, Philipp, Barallobre-Barreiro, Javier, Lengquist, Mariette, Rungger, Gregor, Kapustin, Alexander, Kedenko, Ludmilla, Molenaar, Chris, Lu, Ruifang, Barwari, Temo, Suna, Gonca, Yin, Xiaoke, Iglseder, Bernhard, Paulweber, Bernhard, Willeit, Peter, Shalhoub, Joseph, Pasterkamp, Gerard, Davies, Alun H., Monaco, Claudia, Hedin, Ulf, Shanahan, Catherine M., Willeit, Johann, Kiechl, Stefan, and Mayr, Manuel

Subjects
Atherosclerosis -- Genetic aspects -- Risk factors, Extracellular matrix -- Health aspects, Proteomics, Biological markers -- Identification and classification, Health care industry
Abstract

BACKGROUND. The identification of patients with high-risk atherosclerotic plaques prior to the manifestation of clinical events remains challenging. Recent findings question histology- and imaging-based definitions of the 'vulnerable plaque,' necessitating an improved approach for predicting onset of symptoms. METHODS. We performed a proteomics comparison of the vascular extracellular matrix and associated molecules in human carotid endarterectomy specimens from 6 symptomatic versus 6 asymptomatic patients to identify a protein signature for high-risk atherosclerotic plaques. Proteomics data were integrated with gene expression profiling of 121 carotid endarterectomies and an analysis of protein secretion by lipid-loaded human vascular smooth muscle cells. Finally, epidemiological validation of candidate biomarkers was performed in two community-based studies. RESULTS. Proteomics and at least one of the other two approaches identified a molecular signature of plaques from symptomatic patients that comprised matrix metalloproteinase 9, chitinase 3-like-1, S100 calcium binding protein A8 (S100A8), S100A9, cathepsin B, fibronectin, and galectin-3-binding protein. Biomarker candidates measured in 685 subjects in the Bruneck study were associated with progression to advanced atherosclerosis and incidence of cardiovascular disease over a 10-year follow-up period. A 4-biomarker signature (matrix metalloproteinase 9, S100A8/S100A9, cathepsin D, and galectin-3binding protein) improved risk prediction and was successfully replicated in an independent cohort, the SAPHIR study. CONCLUSION. The identified 4-biomarker signature may improve risk prediction and diagnostics for the management of cardiovascular disease. Further, our study highlights the strength of tissue-based proteomics for biomarker discovery. FUNDING. UK: British Heart Foundation (BHF); King's BHF Center; and the National Institute for Health Research Biomedical Research Center based at Guy's and St Thomas' NHS Foundation Trust and King's College London in partnership with King's College Hospital. Austria: Federal Ministry for Transport, Innovation and Technology (BMVIT); Federal Ministry of Science, Research and Economy (BMWFW); Wirtschaftsagentur Wien; and Standortagentur Tirol., Introduction Atherosclerosis is a chronic and progressive disease of the arterial wall and the main underlying cause of stroke, myocardial infarction (MI), and cardiac death (1, 2). The evolution of [...]

Published
2017
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42. Constructing custom-made radiotranscriptomic signatures of vascular inflammation from routine CT angiograms: a prospective outcomes validation study in COVID-19

Author

Kotanidis, Christos P, primary, Xie, Cheng, additional, Alexander, Donna, additional, Rodrigues, Jonathan C L, additional, Burnham, Katie, additional, Mentzer, Alexander, additional, O’Connor, Daniel, additional, Knight, Julian, additional, Siddique, Muhammad, additional, Lockstone, Helen, additional, Thomas, Sheena, additional, Kotronias, Rafail, additional, Oikonomou, Evangelos K, additional, Badi, Ileana, additional, Lyasheva, Maria, additional, Shirodaria, Cheerag, additional, Lumley, Sheila F, additional, Constantinides, Bede, additional, Sanderson, Nicholas, additional, Rodger, Gillian, additional, Chau, Kevin K, additional, Lodge, Archie, additional, Tsakok, Maria, additional, Gleeson, Fergus, additional, Adlam, David, additional, Rao, Praveen, additional, Indrajeet, Das, additional, Deshpande, Aparna, additional, Bajaj, Amrita, additional, Hudson, Benjamin J, additional, Srivastava, Vivek, additional, Farid, Shakil, additional, Krasopoulos, George, additional, Sayeed, Rana, additional, Ho, Ling-Pei, additional, Neubauer, Stefan, additional, Newby, David E, additional, Channon, Keith M, additional, Deanfield, John, additional, Antoniades, Charalambos, additional, Ahern, David J, additional, Ai, Zhichao, additional, Ainsworth, Mark, additional, Allan, Chris, additional, Allcock, Alice, additional, Angus, Brian, additional, Ansari, M Azim, additional, Arancibia-Cárcamo, Carolina, additional, Aschenbrenner, Dominik, additional, Attar, Moustafa, additional, Baillie, J Kenneth, additional, Barnes, Eleanor, additional, Bashford-Rogers, Rachael, additional, Bashyal, Archana, additional, Beer, Sally, additional, Berridge, Georgina, additional, Beveridge, Amy, additional, Bibi, Sagida, additional, Bicanic, Tihana, additional, Blackwell, Luke, additional, Bowness, Paul, additional, Brent, Andrew, additional, Brown, Andrew, additional, Broxholme, John, additional, Buck, David, additional, Byrne, Helen, additional, Camara, Susana, additional, Ferreira, Ivan Candido, additional, Charles, Philip, additional, Chen, Wentao, additional, Chen, Yi-Ling, additional, Chong, Amanda, additional, Clutterbuck, Elizabeth, additional, Coles, Mark, additional, Conlon, Christopher, additional, Cornall, Richard, additional, Cribbs, Adam, additional, Curion, Fabiola, additional, Davenport, Emma, additional, Davidson, Neil, additional, Davis, Simon, additional, Dendrou, Calliope, additional, Dequaire, Julie, additional, Dib, Lea, additional, Docker, James, additional, Dold, Christina, additional, Dong, Tao, additional, Downes, Damien, additional, Drakesmith, Hal, additional, Dunachie, Susanna, additional, Duncan, David, additional, Eijsbouts, Chris, additional, Esnouf, Robert, additional, Espinosa, Alexis, additional, Etherington, Rachel, additional, Fairfax, Benjamin, additional, Fairhead, Rory, additional, Fang, Hai, additional, Fassih, Shayan, additional, Felle, Sally, additional, Fernandez Mendoza, Maria, additional, Ferreira, Ricardo, additional, Fischer, Roman, additional, Foord, Thomas, additional, Forrow, Aden, additional, Frater, John, additional, Fries, Anastasia, additional, Gallardo Sanchez, Veronica, additional, Garner, Lucy, additional, Geeves, Clementine, additional, Georgiou, Dominique, additional, Godfrey, Leila, additional, Golubchik, Tanya, additional, Gomez Vazquez, Maria, additional, Green, Angie, additional, Harper, Hong, additional, Harrington, Heather, additional, Heilig, Raphael, additional, Hester, Svenja, additional, Hill, Jennifer, additional, Hinds, Charles, additional, Hird, Clare, additional, Hoekzema, Renee, additional, Hollis, Benjamin, additional, Hughes, Jim, additional, Hutton, Paula, additional, Jackson-Wood, Matthew, additional, Jainarayanan, Ashwin, additional, James-Bott, Anna, additional, Jansen, Kathrin, additional, Jeffery, Katie, additional, Jones, Elizabeth, additional, Jostins, Luke, additional, Kerr, Georgina, additional, Kim, David, additional, Klenerman, Paul, additional, Kumar, Vinod, additional, Kumar Sharma, Piyush, additional, Kurupati, Prathiba, additional, Kwok, Andrew, additional, Lee, Angela, additional, Linder, Aline, additional, Lockett, Teresa, additional, Lonie, Lorne, additional, Lopopolo, Maria, additional, Lukoseviciute, Martyna, additional, Luo, Jian, additional, Marinou, Spyridoula, additional, Marsden, Brian, additional, Martinez, Jose, additional, Matthews, Philippa, additional, Mazurczyk, Michalina, additional, McGowan, Simon, additional, McKechnie, Stuart, additional, Mead, Adam, additional, Mi, Yuxin, additional, Monaco, Claudia, additional, Montadon, Ruddy, additional, Napolitani, Giorgio, additional, Nassiri, Isar, additional, Novak, Alex, additional, O'Brien, Darragh, additional, O'Connor, Daniel, additional, O'Donnell, Denise, additional, Ogg, Graham, additional, Overend, Lauren, additional, Park, Inhye, additional, Pavord, Ian, additional, Peng, Yanchun, additional, Penkava, Frank, additional, Pereira Pinho, Mariana, additional, Perez, Elena, additional, Pollard, Andrew, additional, Powrie, Fiona, additional, Psaila, Bethan, additional, Quan, T Phuong, additional, Repapi, Emmanouela, additional, Revale, Santiago, additional, Silva-Reyes, Laura, additional, Richard, Jean-Baptiste, additional, Rich-Griffin, Charlotte, additional, Ritter, Thomas, additional, Rollier, Christine, additional, Rowland, Matthew, additional, Ruehle, Fabian, additional, Salio, Mariolina, additional, Sansom, Stephen Nicholas, additional, Sanches Peres, Raphael, additional, Santos Delgado, Alberto, additional, Sauka-Spengler, Tatjana, additional, Schwessinger, Ron, additional, Scozzafava, Giuseppe, additional, Screaton, Gavin, additional, Seigal, Anna, additional, Semple, Malcolm, additional, Sergeant, Martin, additional, Simoglou Karali, Christina, additional, Sims, David, additional, Skelly, Donal, additional, Slawinski, Hubert, additional, Sobrinodiaz, Alberto, additional, Sousos, Nikolaos, additional, Stafford, Lizzie, additional, Stockdale, Lisa, additional, Strickland, Marie, additional, Sumray, Otto, additional, Sun, Bo, additional, Taylor, Chelsea, additional, Taylor, Stephen, additional, Taylor, Adan, additional, Thongjuea, Supat, additional, Thraves, Hannah, additional, Todd, John, additional, Tomic, Adriana, additional, Tong, Orion, additional, Trebes, Amy, additional, Trzupek, Dominik, additional, Tucci, Felicia Anna, additional, Turtle, Lance, additional, Udalova, Irina, additional, Uhlig, Holm, additional, van Grinsven, Erinke, additional, Vendrell, Iolanda, additional, Verheul, Marije, additional, Voda, Alexandru, additional, Wang, Guanlin, additional, Wang, Lihui, additional, Wang, Dapeng, additional, Watkinson, Peter, additional, Watson, Robert, additional, Weinberger, Michael, additional, Whalley, Justin, additional, Witty, Lorna, additional, Wray, Katherine, additional, Xue, Luzheng, additional, Yuen Yeung, Hing, additional, Yin, Zixi, additional, Young, Rebecca, additional, Youngs, Jonathan, additional, Zhang, Ping, additional, Zurke, Yasemin-Xiomara, additional, Banning, Adrian, additional, Antonopoulos, Alexios, additional, Kelion, Andrew, additional, Kardos, Attila, additional, Hudson, Benjamin, additional, Koo, Bon-Kwon, additional, Kotanidis, Christos, additional, Mahon, Ciara, additional, Berry, Colin, additional, Newby, David, additional, Connolly, Derek, additional, Scaletta, Diane, additional, Nicol, Ed, additional, McAlindon, Elisa, additional, Oikonomou, Evangelos, additional, Pugliese, Francesca, additional, Pontone, Gianluca, additional, Benedetti, Giulia, additional, He, Guo-Wei, additional, West, Henry, additional, Kondo, Hidekazu, additional, Benedek, Imre, additional, Das, Intrajeet, additional, Graby, John, additional, Greenwood, John, additional, Rodrigues, Jonathan, additional, Ge, Junbo, additional, Channon, Keith, additional, Fabritz, Larissa, additional, Fan, Li-Juan, additional, Kingham, Lucy, additional, Guglielmo, Marco, additional, Schmitt, Matthias, additional, Beer, Meinrad, additional, Anderson, Michelle, additional, Desai, Milind, additional, Marwan, Mohamed, additional, Takahashi, Naohiko, additional, Mehta, Nehal, additional, Dai, Neng, additional, Screaton, Nicholas, additional, Sabharwal, Nikant, additional, Maurovich-Horvat, Pál, additional, Kharbanda, Rajesh, additional, Preston, Rebecca, additional, Wood, Richard, additional, Blankstein, Ron, additional, Rajani, Ronak, additional, Mirsadraee, Saeed, additional, Munir, Shahzad, additional, Klömpken, Steffen, additional, Petersen, Steffen, additional, Achenbach, Stephan, additional, Anthony, Susan, additional, Mak, Sze, additional, Mittal, Tarun, additional, Benedek, Theodora, additional, Sharma, Vinoda, additional, and Lin, Wen-Hua, additional

Published
2022
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45. Anti-TNF Therapy

Author

Udalova, Irina, primary, Monaco, Claudia, additional, Nanchahal, Jagdeep, additional, and Feldmann, Marc, additional

Published
2017
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46. Myeloid CD40 deficiency reduces atherosclerosis by impairing macrophages' transition into a pro-inflammatory state.

Author

Bosmans, Laura A, Tiel, Claudia M van, Aarts, Suzanne A B M, Willemsen, Lisa, Baardman, Jeroen, Os, Bram W van, Toom, Myrthe den, Beckers, Linda, Ahern, David J, Levels, Johannes H M, Jongejan, Aldo, Moerland, Perry D, Verberk, Sanne G S, van den Bossche, Jan, Winther, Menno M P J de, Weber, Christian, Atzler, Dorothee, Monaco, Claudia, Gerdes, Norbert, and Shami, Annelie

Subjects
INFLAMMATION, ATHEROSCLEROTIC plaque, MYELOID cells, ATHEROSCLEROSIS, MACROPHAGES, CYTOMETRY
Abstract

Aims CD40 and its ligand, CD40L, play a critical role in driving atherosclerotic plaque development. Disrupted CD40-signalling reduces experimental atherosclerosis and induces a favourable stable plaque phenotype. We recently showed that small molecule-based inhibition of CD40-tumour necrosis factor receptor associated factor-6 interactions attenuates atherosclerosis in hyperlipidaemic mice via macrophage-driven mechanisms. The present study aims to detail the function of myeloid CD40 in atherosclerosis using myeloid-specific CD40-deficient mice. Method and Results Cd40flox/flox and LysM-cre Cd40flox/flox mice on an Apoe−/− background were generated (CD40wt and CD40mac−/− , respectively). Atherosclerotic lesion size, as well as plaque macrophage content, was reduced in CD40mac−/− compared to CD40wt mice, and their plaques displayed a reduction in necrotic core size. Transcriptomics analysis of the CD40mac−/− atherosclerotic aorta revealed downregulated pathways of immune pathways and inflammatory responses. Loss of CD40 in macrophages changed the representation of aortic macrophage subsets. Mass cytometry analysis revealed a higher content of a subset of alternative or resident-like CD206+CD209b− macrophages in the atherosclerotic aorta of CD40mac−/− compared to CD40wt mice. RNA-sequencing of bone marrow-derived macrophages of CD40mac−/− mice demonstrated upregulation of genes associated with alternatively activated macrophages (including Folr2 , Thbs1 , Sdc1 , and Tns1). Conclusions We here show that absence of CD40 signalling in myeloid cells reduces atherosclerosis and limits systemic inflammation by preventing a shift in macrophage polarization towards pro-inflammatory states. Our study confirms the merit of macrophage-targeted inhibition of CD40 as a valuable therapeutic strategy to combat atherosclerosis. [ABSTRACT FROM AUTHOR]

Published
2023
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48. Preclinical Aortic Atherosclerosis in Adolescents With Chronic Disease

Author

Ververs, Francesca A., primary, Eikendal, Anouk L. M., additional, Kofink, Daniel, additional, Nuboer, Roos, additional, Westenberg, Jos J. M., additional, Hovenkamp, Gijs T., additional, Kemps, Jitske J.A., additional, Coenen, Iris C. J., additional, Daems, Joëlle J. N., additional, Claus, Laura R., additional, Ju, Yillie, additional, Wulffraat, Nico M., additional, van der Ent, Cornelis K., additional, Monaco, Claudia, additional, Boes, Marianne, additional, Leiner, Tim, additional, Grotenhuis, Heynric B., additional, and Schipper, Henk S., additional

Published
2022
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49. Myeloid CD40 deficiency reduces atherosclerosis by impairing macrophages’ transition into a pro-inflammatory state

Author

Bosmans, Laura A, primary, van Tiel, Claudia M, additional, Aarts, Suzanne A B M, additional, Willemsen, Lisa, additional, Baardman, Jeroen, additional, van Os, Bram W, additional, Toom, Myrthe den, additional, Beckers, Linda, additional, Ahern, David J, additional, Levels, Johannes H M, additional, Jongejan, Aldo, additional, Moerland, Perry D, additional, Verberk, Sanne G S, additional, den Bossche, Jan van, additional, de Winther, Menno M P J, additional, Weber, Christian, additional, Atzler, Dorothee, additional, Monaco, Claudia, additional, Gerdes, Norbert, additional, Shami, Annelie, additional, and Lutgens, Esther, additional

Published
2022
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50. Genetic Deficiency of Indoleamine 2,3-dioxygenase Aggravates Vascular but Not Liver Disease in a Nonalcoholic Steatohepatitis and Atherosclerosis Comorbidity Model

Author

Arora, Aastha, primary, Tripodi, Gustavo Luis, additional, Kareinen, Ilona, additional, Berg, Martin, additional, Forteza, Maria Josefa, additional, Gisterå, Anton, additional, Griepke, Silke, additional, Casagrande, Felipe Beccaria, additional, Martins, Joilson O., additional, Abdalla, Dulcineia Saes Parra, additional, Cole, Jennifer, additional, Monaco, Claudia, additional, and Ketelhuth, Daniel F. J., additional

Published
2022
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